Vascular tissue engineering by computer-aided laser micromachining by Anand Doraiswamy, and Roger J....

Vascular tissue engineering by computer-aided laser micromachining

by Anand Doraiswamy, and Roger J. Narayan

Philosophical Transactions AVolume 368(1917):1891-1912

April 28, 2010

©2010 by The Royal Society

Schematic of the computer-aided laser micromachining process.

Anand Doraiswamy, and Roger J. Narayan Phil. Trans. R. Soc. A 2010;368:1891-1912


(a) Top-view optical micrograph of channels fabricated in 1% agarose hydrogel.



(a) Cross-sectional optical micrograph of channels that were prepared using several apertures (scale bar, 500 μm).



(a) Optical micrograph of a two-circle ring.



Computer-aided laser micromachining process from the CAD to the cell-seeded structure.



MTT cell viability of HA-VSMC human aortic smooth muscle cells and HAAE-1 human aortic endothelial cells for various amounts of vascular endothelial growth factor (1–1000 ng ml−1);

these data were normalized with cell viability on unmodified media.



MTT cell viability of HA-VSMC human aortic smooth muscle cells and HAAE-1 human aortic endothelial cells for various amounts of heparin (1–1000 units ml−1); these data were normalized

with cell viability on unmodified media.



MTT cell viability of HA-VSMC human aortic smooth muscle cells and HAAE-1 human aortic endothelial cells for Matrigel as well as Matrigel containing 10 ng ml−1 vascular endothelial

growth factor (VEGF) and 100 units ml−1 heparin; these data were normalized ...



(a) Optical micrograph of HA-VSMC human aortic smooth muscle cells grown in Matrigel containing 10 ng ml−1 vascular endothelial growth factor and 100 units ml−1 heparin.



Optical micrograph of a stained agarose channel that was filled with HA587 human elastin.



(a) Optical micrograph of a fully grown HA-VSMC human aortic smooth muscle cell–Matrigel network.



(a) Optical micrograph of vascular network containing HAAE-1 human aortic endothelial cells, HA-VSMC human aortic smooth muscle cells, human elastin as well as Matrigel containing 10 ng

ml−1 of vascular endothelial growth factor and 100 units ml−1 of heparin.



(a) Live/dead stained fluorescent micrograph of vascular network containing HA-VSMC human aortic smooth muscle cells and Matrigel.



Vascular tissue engineering by computer-aided laser micromachining by Anand Doraiswamy, and Roger J....

Documents

Transcript of Vascular tissue engineering by computer-aided laser micromachining by Anand Doraiswamy, and Roger J....