Varispenser plus - Eppendorf · The Varispenser plus has two dispensing settings and one adjustment...

nual/Varispenser® plusEN)g manual

Register your instrument! www.eppendorf.com/myeppendorf

Varispenser®/Varispenser® plus

Operating manual

Copyright © 2015 Eppendorf AG, Hamburg. All rights reserved, including graphics and images. No part of this publication may be reproduced without the prior permission of the copyright owner.

Eppendorf® and the Eppendorf logo are registered trademarks of Eppendorf AG, Germany.

Varispenser® is a registered trademark of Eppendorf AG, Hamburg, Germany.

Registered trademarks and protected trademarks are not marked in all cases with ® or ™ in this manual.

4960 900.028-10/052015

Table of contentsVarispenser®/Varispenser® plus

English (EN)3

Table of contents

1 Operating instructions. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51.1 Using this manual . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51.2 Danger symbols and danger levels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5

1.2.1 Danger symbols . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51.2.2 Danger levels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5

1.3 Symbols used . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5

2 Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62.1 Intended use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62.2 User profile . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62.3 Warnings for intended use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 72.4 Information on product liability . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8

3 Product description . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 93.1 Varispenser main illustration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 93.2 Varispenser plus main illustration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 103.3 Features. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 113.4 Delivery package . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 113.5 Materials . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12

4 Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 134.1 Connecting the thread adapter . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13

4.1.1 Selecting the thread adapter . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 134.1.2 Connecting the thread adapter . . . . . . . . . . . . . . . . . . . . . . . . . . . 13

4.2 Connecting the telescopic aspirating tube . . . . . . . . . . . . . . . . . . . . . . . . . . 144.2.1 Extending the telescopic aspirating tube . . . . . . . . . . . . . . . . . . . 144.2.2 Shortening the telescopic aspirating tube. . . . . . . . . . . . . . . . . . . 144.2.3 Mounting the telescopic aspirating tube. . . . . . . . . . . . . . . . . . . . 15

4.3 Connecting the device. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 164.4 Connecting the drying tube . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17

5 Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 185.1 Carrying the device . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 185.2 Checking the device before use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 185.3 Venting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19

5.3.1 Venting the Varispenser . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 195.3.2 Venting the Varispenser plus. . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20

5.4 Setting the volume . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 215.5 Dispensing. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 225.6 Recirculating liquid with the Varispenser plus . . . . . . . . . . . . . . . . . . . . . . . 23

4Table of contentsVarispenser®/Varispenser® plusEnglish (EN)

6 Gravimetric test . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 246.1 Calibrating the Varispenser/Varispenser plus. . . . . . . . . . . . . . . . . . . . . . . . 24

6.1.1 Measuring the testing volume. . . . . . . . . . . . . . . . . . . . . . . . . . . . 246.1.2 Calculating the error . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 256.1.3 Evaluating the calibration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25

6.2 Calibrating the Varispenser plus for other liquid densities . . . . . . . . . . . . . 266.3 Adjusting the Varispenser plus . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27

7 Troubleshooting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 287.1 Troubleshooting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28

8 Maintenance. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 308.1 Decontamination before shipment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 308.2 Cleaning the Varispenser/Varispenser plus . . . . . . . . . . . . . . . . . . . . . . . . . 30

8.2.1 Emptying the device . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 318.2.2 Standard cleaning . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 328.2.3 Intensive cleaning . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33

8.3 Autoclaving the device . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 338.4 Removing the filling valve. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 348.5 Installing the filling valve . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34

9 Technical data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 359.1 Ambient conditions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 359.2 Errors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35

10 Ordering information. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3710.1 Devices . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3710.2 Spare parts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3810.3 Threaded adapter . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39

11 Transport, storage and disposal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4011.1 Transport . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4011.2 Storage . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4011.3 Disposal. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 40

12 Measurement report . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41

Operating instructionsVarispenser®/Varispenser® plus

English (EN)5

1 Operating instructions1.1 Using this manual

Read this operating manual completely before using the device for the first time. Also observe the instructions for use of the accessories.

This operating manual is part of the product. Thus, it must always be easily accessible.Enclose this operating manual when transferring the device to third parties.You will find the current version of the operating manual for all available languages on

our webpage under www.eppendorf.com.

1.2 Danger symbols and danger levels

The safety instructions of this operating manual indicate the following danger symbols and danger levels:

1.2.1 Danger symbols

1.2.2 Danger levels

1.3 Symbols used

Biohazard Toxic substances

Hazard point Material damage

DANGER Will lead to severe injuries or death.

WARNING May lead to severe injuries or death.

CAUTION May lead to light to moderate injuries.

NOTICE May lead to material damage.

Depiction Meaning

1.2.

Actions in the specified order

Actions without a specified order

• List

Text Display text or software text

Additional information

6SafetyVarispenser®/Varispenser® plusEnglish (EN)

2 Safety2.1 Intended use

The Varispenser/Varispenser plus is a laboratory device for dispensing aqueous solutions in various volume ranges directly from a storage bottle. In vivo applications (applications in or on the human body) are not permitted.

The device is not suitable for the following solutions, substances and liquids:• Liquids which attack FEP, ETFE, PFA, PTFE, PVDF and PP• Solutions which contain hydrofluoric acid as these solutions attack borosilicate glass• Suspensions as these contain solid particles• Solutions which decompose or crystallize out and form solid particles (e.g., biuret

reagent)• Substances which react catalytically with platinum iridium (e.g., H2O2)

• Carbon disulfide as it is easily flammable• Trifluoroacetic acid• Tetrahydrofuran• Use nitric acid with a concentration > 60 % only with PTFE thread adapters

For the following substances the device is suitable to a limited extent:• Liquids which form deposits can make the piston difficult to move or get stuck.• Avoid electrostatic charging of the device and vessels when dispensing flammable

substances.

Physical limitations for device and liquid:• Ambient temperature 15 °C to 40 °C

• Density up to 2.2 g/cm³

• Vapor pressure up to 500 mbar. Aspirate slowly above 300 mbar to avoid boiling of the liquid.

• Kinematic viscosity up to 500 mm²/s

2.2 User profile

The device and accessories may only be operated by trained and skilled personnel.

Before using the device, read the operating manual carefully and familiarize yourself with the device's mode of operation.

SafetyVarispenser®/Varispenser® plus

English (EN)7

2.3 Warnings for intended use

WARNING! Damage to health due to infectious liquids and pathogenic germs.

When handling infectious liquids and pathogenic germs, observe the national regulations, the biological security level of your laboratory, the material safety data sheets, and the manufacturer's application notes.

Wear personal protective equipment.For comprehensive regulations about handling germs or biological material

of risk group II or higher, please refer to the "Laboratory Biosafety Manual" (source: World Health Organization, Laboratory Biosafety Manual, in its respectively current valid version).

WARNING! Damage to health due to toxic, radioactive or aggressive chemicals.

Wear personal protective equipment.Observe the national regulations for handling these substances. Observe the material safety data sheets and manufacturer's application notes.

CAUTION! Contamination due to contact with biological and chemical reagents.Contact with reagents may be harmful to eyes or skin.

Wear personal protective equipment.Ensure that no reagents are escaping from the device. Ensure that the piston can move freely before starting work.Do not point the opening of the discharge tube at people.Remove the sealing cap from the discharge tube before pushing the piston

down.Only initiate the dispensing process if this does not pose a danger to people.Dispense liquid slowly and evenly to prevent splashing. Do not use force.Only dismantle the device if it has been cleaned.

CAUTION! Contamination with reagents when removing the sealing cap.The sealing cap may contain biological and chemical reagents. Contact with reagents may be harmful to eyes or skin.

Wear your personal protective equipment when removing the sealing cap.

8SafetyVarispenser®/Varispenser® plusEnglish (EN)

2.4 Information on product liability

In the following cases, the designated protection of the device may be compromised. Liability for any resulting property damage or personal injury is then transferred to the operator:

• The device is not used in accordance with the operating manual.• The device is used outside of its intended use.• The device is used with accessories or consumables which are not recommended by

Eppendorf.• The device is maintained or repaired by people not authorized by Eppendorf.• The user makes unauthorized changes to the device.

CAUTION! Personal injury due to incorrect transportation of the device.If the assembled device is not transported properly, reagents may be released. Contact with reagents may be harmful to eyes or skin.

To transport the assembled device, grasp the head gear of the device with one hand and support the bottom of the bottle with the other hand.

Do not grasp the device at the cylinder sleeve.

CAUTION! Poor safety due to incorrect accessories and spare parts.The use of accessories and spare parts other than those recommended by Eppendorf may impair the safety, functioning and precision of the device. Eppendorf cannot be held liable or accept any liability for damage resulting from the use of incorrect or non-recommended accessories and spare parts, or from the improper use of such equipment.

Only use accessories and original spare parts recommended by Eppendorf.

NOTICE! Material damage from incorrect use.

Only use the product for its intended purpose as described in the operating manual.

Ensure adequate material resistance when using chemical substances. In case of doubt, contact the manufacturer of this product.

NOTICE! Damage to device due to contamination in the deviceIf there is contamination in the device, the dispensing valve may become blocked. When the piston is pushed down, a high pressure develops in the device. This pressure may cause the ball to be released from the valve and destroy the sealing lip. Liquid can penetrate into the device.

Ensure that the piston can move freely before using the device.Stop dispensing if the piston is difficult to move.Clean the device.

Product descriptionVarispenser®/Varispenser® plus

English (EN)9

3 Product description3.1 Varispenser main illustrationAbb. 3-1:Varispenser

Fig. 3-1: Varispenser

1 Piston mount

2 Cylinder sleeve

3 Volume selection slider

4 Piston

5 Cylinder with protective shell

6 Canula arm

7 Discharge tube

8 Sealing cap

9 Telescopic aspirating tube

10 Filling valve

11 Head gear

12 Ventilation cover

1

2

3

4

5

7

8

12

9

11

10

6

10Product descriptionVarispenser®/Varispenser® plusEnglish (EN)

3.2 Varispenser plus main illustrationAbb. 3-2:Varispenser plus

Fig. 3-2: Varispenser plus

1 Adjustment device

2 Piston mount

3 Cylinder sleeve

4 Volume selection slider

5 Piston

6 Cylinder with protective shell

7 Valve switch

8 Canula arm

9 Discharge tube

10 Sealing cap

11 Telescopic aspirating tube

12 Filling valve

13 Head gear

14 Ventilation cover

3

4

5

6

7

1

2

9

10

14

8

12

11

13

Product descriptionVarispenser®/Varispenser® plus

English (EN)11

3.3 Features

The Varispenser is a bottle top dispenser for dispensing liquids with milliliter accuracy without wasting liquid.

When handling aggressive liquids, the Varispenser offers the best possible protection for users and the laboratory. The user has to judge for himself whether the Varispenser is suitable for the intended application. The user has to judge the chemical compatibility of the used reagents himself.

The Varispenser plus has two dispensing settings and one adjustment device:

• Dispensing Dispense liquid from a bottle into a destination vessel.

• Recirculation Recirculate liquid from the bottle back into the bottle, e.g., to remove air bubbles from the cylinder.

• Adjustment deviceSet the Varispenser plus to liquids with a density other than water.

The Varispenser is autoclavable.

3.4 Delivery package

Depending on your order you have purchased one of the following devices with accessories.

Order no. (International)

Order no. (North America)

Description

VarispenserBottle top dispenser with telescopic aspirating tube, universal wrench, and 3 adapters

4960 000.019 4960000019 0,5 mL - 2,5 mL; for bottle thread 32 mm; adapter 28 mm, 40 mm and 45 mm

4960 000.027 4960000027 1 mL - 5 mL; for bottle thread 32 mm; adapter 28 mm, 40 mm and 45 mm





12Product descriptionVarispenser®/Varispenser® plusEnglish (EN)

3.5 Materials

Varispenser plusBottle top dispenser with return valve, valve switch, and media-dependant fine adjustment. With telescopic aspirating tube, universal wrench, and 3 adapters

4961 000.012 4961000012 0.5 mL - 2.5 mL; for bottle thread 32 mm; adapter 28 mm, 40 mm and 45 mm






NOTICE! Aggressive substances may damage the Varispenser and accessories.

Check the resistance to chemicals before using organic solvents or aggressive chemicals.

Parts which come into contact with the liquid Parts which do not come into contact with the liquid

• Borosilicate glass 3.3 (boro 3.3)• Ethylene tetrafluoroethylene (ETFE)• Perfluoroalkoxy (PFA)• Platinum iridium (Pt-Ir)• Polytetrafluoroethylene (PTFE)• Polyvinylidene difluoride (PVDF)• Tetrafluoroethylene perfluoropropylene (FEP)

• Ethylene tetrafluoroethylene (ETFE)• Fluororubber (FKM)• Polypropylene (PP)



Description

InstallationVarispenser®/Varispenser® plus

English (EN)13

4 Installation4.1 Connecting the thread adapter

The following devices can be screwed directly onto a bottle:

Use a thread adapter for flask necks with other thread sizes.

4.1.1 Selecting the thread adapter

1. Measure the inner diameter of the bottle lid.2. Select a thread adapter which comes closest to the measured diameter.

If you need a thread adapter with a high resistance to chemicals, use a PTFE/ETFE adapter.

4.1.2 Connecting the thread adapter

1. Screw the thread adapter onto the flask neck.2. Ensure that the adapter is firmly connected to the flask neck.

Varispenser/Varispenser plus Matching bottle thread

2.50 mL, 5.00 mL, 10.0 mL 32 mm thread

25.0 mL, 50.0 mL, 100.0 mL 45 mm thread

14InstallationVarispenser®/Varispenser® plusEnglish (EN)

4.2 Connecting the telescopic aspirating tube4.2.1 Extending the telescopic aspirating tube

Prerequisites• The bottle is larger than the collapsed telescopic aspirating tube.

4.2.2 Shortening the telescopic aspirating tube

Prerequisites• The bottle is smaller than the collapsed telescopic aspirating tube.

1. Pull the inner tube completely out of the outer tube.2. Shorten both tubes to the length of the bottle with a pair of scissors.3. Slide the inner tube into the outer tube.

Abb. 4-1:Telescopic aspirating tube in bottle

Fig. 4-1: Telescopic aspirating tube in bottle

Extend the inner and outer tube until the tube is slightly longer than the bottle.


English (EN)15

4.2.3 Mounting the telescopic aspirating tube

Take care not to bend the telescopic aspirating tube during mounting.

1. Slide the appropriate end of the tube onto the filling valve up to the stop.

2. Cut off the end of the telescopic aspirating tube at an acute angle.

16InstallationVarispenser®/Varispenser® plusEnglish (EN)

4.3 Connecting the device

Prerequisites• The telescopic aspirating tube has been mounted.• The length of the telescopic aspirating tube has been adjusted to the length of the

bottle.

1. Position the device on the bottle thread or thread adapter.

2. Screw the device onto the bottle thread or thread adapter.

3. Ensure that the device is firmly attached to the bottle.


English (EN)17

4.4 Connecting the drying tube

To dispense hygroscopic liquids you need a drying tube. The drying tube must be filled with suitable liquid absorbers.

Suitable liquid absorbers• Silica gel with a particle size of 1 mm – 3 mm• CaCO2

• NaOH pills (5 mm ø, for the absorption of CO2)

1. Remove the ventilation cover.2. Slide the drying tube into the vent.

18OperationVarispenser®/Varispenser® plusEnglish (EN)

5 Operation5.1 Carrying the device

5.2 Checking the device before use

Check the condition of the device as follows:

1. Ensure that the piston can be pulled up and pushed down easily. 2. Ensure that no liquid leaks from the device.3. Ensure that all parts of the device are firmly in position.

If any problems occur during the check, do not use the device. Refer to the Troubleshooting chapter (see p. 28).

CAUTION! Personal injury due to incorrect transportation of the device.If the assembled device is not transported properly, reagents may be released. Contact with reagents may be harmful to eyes or skin.

To transport the assembled device, grasp the head gear of the device with one hand and support the bottom of the bottle with the other hand.

Do not grasp the device at the cylinder sleeve.

Abb. 5-1:Carrying the assembled device

Fig. 5-1: Carrying the assembled device

1. Grasp the dispenser with one hand.2. Support the dispenser and the bottle

with the other hand. To do so, place your hand under the bottom of the bottle.

OperationVarispenser®/Varispenser® plus

English (EN)19

5.3 Venting

Air bubbles in the supply lines and in the cylinder falsify the amount of dispensed liquid.

Vent the device in the following situations:• Prior to initial setup• After intensive cleaning• After changing the bottle• After a long period of inactivity

5.3.1 Venting the Varispenser

Prerequisites• The Varispenser has been mounted.• The piston is in the lower position.

CAUTION! Personal injury due to contact with reagents.Contact with reagents may be harmful to eyes or skin.

Wear personal protective equipment (PPE).Do not point the discharge tube at people.

1. Remove the sealing cap.2. Pull the piston up 2 cm.3. Hold a collection vessel under the

discharge tube.4. Push the piston firmly down.5. Repeat steps 2 to 4 until no more air

bubbles are visible in the cylinder.


5.3.2 Venting the Varispenser plus

Prerequisites• The Varispenser plus has been mounted.• The piston is in the lower position.

1. Set the valve switch to recirculation .2. Pull the piston up 2 cm.3. Push the piston firmly down.4. Repeat steps 2 to 3 until no more air

bubbles are visible in the cylinder.

5. Remove the sealing cap.6. Hold a collection vessel under the

discharge tube.7. Set the valve switch to dispensing .8. To vent the discharge tube dispense

liquid once.


English (EN)21

5.4 Setting the volume

1. Push the piston all the way down.

2. To release the lock, move the volume selection slider to the right.

3. To set the desired volume, move the volume selection slider up or down.


5.5 Dispensing

Prerequisites• The device has been mounted.• The device has been checked (see p. 18).• The device has been vented (see p. 19).• The volume has been set (see p. 21).

4. Move the volume selection slider to the left as far as it will go.The volume selection slider locks into place.

NOTICE! Damage to device due to contamination in the deviceIf there is contamination in the device, the dispensing valve may become blocked. When the piston is pushed down, a high pressure develops in the device. This pressure may cause the ball to be released from the valve and destroy the sealing lip. Liquid can penetrate into the device.

Ensure that the piston can move freely before using the device.Stop dispensing if the piston is difficult to move.Clean the device.

CAUTION! Contamination with reagents when removing the sealing cap.The sealing cap may contain biological and chemical reagents. Contact with reagents may be harmful to eyes or skin.

Wear your personal protective equipment when removing the sealing cap.


English (EN)23

5.6 Recirculating liquid with the Varispenser plus

With the Varispenser plus you can recirculate liquid from the cylinder back into the bottle (see p. 20).

Prerequisites• The Varispenser plus has been mounted.

1. Only Varispenser plus: Set the valve switch to dispensing .

2. Remove the sealing cap.3. Slowly and evenly pull the piston up until

the stop.4. Hold a collection vessel under the

discharge tube.5. Slowly and evenly push the piston down

until the stop. Do not use force.6. Only Varispenser plus: Set the valve

switch to recirculation after dispensing.

7. Attach the sealing cap after dispensing.

1. Set the valve switch to recirculation .2. Pull the piston up.3. Push the piston down.

The liquid is dispensed back into the bottle.

24Gravimetric testVarispenser®/Varispenser® plusEnglish (EN)

6 Gravimetric test6.1 Calibrating the Varispenser/Varispenser plus

The device was calibrated with demineralized water. You can perform a gravimetric test of the device error. The procedure is described in detail in DIN EN ISO 8655-6.

You need the following auxiliary equipment:• Demineralized water• Calibrated analytical balance• Collection vessel

6.1.1 Measuring the testing volume

Prerequisites• Devices and auxiliary equipment have a temperature of 20 °C to 25 °C.• The temperature difference between the devices and the auxiliary equipment is max.

±0.5 °C.• The device has been vented.• The device has been cleaned according to the standard cleaning procedure.

Proceed as follows:

1. Set up the calibrated analytical balance in a vibration and draft-free place.2. Place a collection vessel on the balance.3. Set the analytical balance to 0.4. Set the device to the maximum volume.5. Dispense demineralized water into the collection vessel and note the displayed weight.6. Set the analytical balance to 0.7. Repeat steps 5 and 6 10 times.

Gravimetric testVarispenser®/Varispenser® plus

English (EN)25

6.1.2 Calculating the error

6.1.3 Evaluating the calibration

1. Compare the results for the systematic error and the random error with the table (see p. 35).

2. If the error is within the specified tolerance, no adjustment is necessary.3. If the error is outside the tolerance, adjust the device (only Varispenser plus) or contact

the authorized service.

1. Calculate the volume for each measurement.• Z = correction factor

Take the correction factor from DIN EN ISO 8655-6.• Vi = calculated volume of the individual measurement

• mi = liquid mass of the individual measurement

• i = 1 to 10

2. Calculate the average value of the volumes.

3. Calculate the systematic error (es) using the nominal volume (Vs).

• = actual volume (average value of the calculated volumes)• Vs = nominal volume

• es = systematic error

4. Calculate the standard deviation.• sr = standard deviation

• n = number of dispensing steps• Vi = individual volume (calculated volume of one measurement)

• = average value of the calculated measurement volumes

5. Calculate the random error.CV = random error

V = m × Z

V = 1/n Vi ∑n

i = 1

V

es = × 100 %V – Vs

Vs

V

(Vi – V)2∑n

i = 1n – 1

sr =

V

100 % × srCV =

26Gravimetric testVarispenser®/Varispenser® plusEnglish (EN)

6.2 Calibrating the Varispenser plus for other liquid densities

You need the following auxiliary equipment:• Demineralized water• Calibrated analytical balance• Collection vessel

1. Set up the calibrated analytical balance in a vibration and draft-free place.2. Place a collection vessel on the analytical balance.3. Set the analytical balance to 0.4. Set the Varispenser plus to the maximum volume.5. Dispense the test liquid into the collection vessel with the Varispenser plus and note

the weight.6. Set the analytical balance to 0.7. Repeat steps 5 and 6 10 times.

10.If the dispensed volume does not match the set volume, adjust the Varispenser plus.

8. Calculate the individual volume of the liquid.• Vi = calculated volume of the individual measurement

• mi = liquid mass of the individual measurement

• i = 1 to 10• ρ = density of the liquid

9. Calculate the average value of the volumes.

• = actual volume (average value of the calculated volumes)

• n = number of dispensing steps

V =ρm

V V = 1/n Vi ∑n

i = 1

Gravimetric testVarispenser®/Varispenser® plus

English (EN)27

6.3 Adjusting the Varispenser plus

The Varispenser plus has been calibrated and adjusted before delivery.

Adjust the device in the following situations:• The calibration error with demineralized water is outside the tolerance range• The density of the dispensed liquid differs from the density of demineralized water

Adjust the Varispenser plus with the adjustment device. One notch of the adjustment device is the smallest adjustment step.

1. To release the lock, push the marked area of the adjustment device in.2. Adjust the Varispenser plus.

3. Check the volume (see p. 26).

Devices Smallest adjustment step Maximum adjustment range

Up to 10 mL Approx. 0.1% of the nominal volume

2% of the nominal volume

25 mL or more Approx. 0.05% of the nominal volume

1% of the nominal volume

Enlarge volume Turn the adjustment device towards +.

Reduce volume Turn the adjustment device towards –.

28TroubleshootingVarispenser®/Varispenser® plusEnglish (EN)

7 Troubleshooting7.1 Troubleshooting

Problem Cause Solution

The piston is difficult to move.

• The sealing cap is on the discharge tube.

Remove the sealing cap.

• Crystals have formed. 1. Interrupt the dispensing procedure immediately.

2. Perform intensive cleaning procedure.

3. If the problem persists, send the device in for repair.

• The piston seal is damaged.

Send in the device for repair.

The aspirated liquid contains air bubbles.

• The device was not sufficiently vented.

Deair the device .

• The telescopic aspirating tube has not been mounted correctly.

Slide the telescopic aspirating tube firmly onto the filling valve .

• The telescopic aspirating tube is damaged.

Shorten the telescopic aspirating tube or replace it .

• The filling valve is damaged.


2. Tighten the filling valve with the universal wrench.

3. If the problem persists, replace the filling valve.

• The telescopic aspirating tube is not immersed in the liquid.

Lengthen the telescopic aspirating tube until it is immersed in the liquid .

• The bottle is empty. Fill the bottle.

The device does not aspirate any liquid.



The device does not aspirate any liquid.

• The filling valve is clogged.



TroubleshootingVarispenser®/Varispenser® plus

English (EN)29

The dispensed volume is too low.



• The telescopic aspirating tube is damaged.

Shorten the telescopic aspirating tube or replace it .

• The device is not adjusted correctly.

1. Only Varispenser plus: Adjust the device.

2. If the device cannot be adjusted or the problem persists, send the device in for repair.

• The filling valve is damaged.


2. Tighten the filling valve with the universal wrench.


Problem Cause Solution

30MaintenanceVarispenser®/Varispenser® plusEnglish (EN)

8 Maintenance8.1 Decontamination before shipment

If you are shipping the device to the authorized Technical Service for repairs or to your authorized dealer for disposal please note the following:

8.2 Cleaning the Varispenser/Varispenser plus

Clean the Varispenser in the following situations:

WARNING! Risk to health from contaminated device

1. Follow the instructions in the decontamination certificate. You find it as a PDF file on our website (www.eppendorf.com/decontamination).

2. Decontaminate all the parts you would like to dispatch.3. Include the fully completed decontamination certificate in the package.

CAUTION! Personal injury due to contact with reagents.The feeding mechanics, valves, telescopic aspirating tube and discharge tube are filled with reagents. Reagents enter the flask with the cleaning solution.Contact with reagents may be harmful to eyes or skin.

Wear personal protective equipment (PPE).Discard the cleaning solution after cleaning.

Standard cleaning Intensive cleaning

• Prior to changing the reagent.• Prior to storage.• Prior to service and repair work.

• If the piston is difficult to move.• Prior to autoclaving.• In case of severe crystallization.

MaintenanceVarispenser®/Varispenser® plus

English (EN)31

8.2.1 Emptying the device

Prerequisites• The piston is in the lower position.• Only Varispenser plus: The valve switch is set to dispensing .

1. Attach the sealing cap.2. Place the device and bottle into a

collecting vessel.3. Unscrew the device from the bottle.4. Pull the device out of the bottle until the

telescopic aspirating tube is no longer immersed in the liquid.

5. Gently tap the telescopic aspirating tube against the inside of the bottle.The residual liquid flows out of the telescopic aspirating tube.


8.2.2 Standard cleaning

Prerequisites• The device is empty.• Only Varispenser plus: The valve switch is set to dispensing .

1. Screw the device onto a bottle with a neutral cleaning solution.2. Place a collection vessel below the discharge tube.3. Remove the sealing cap.4. Flush the device by pumping several times.

5. Only Varispenser plus: Set the valve switch to recirculation . Flush the recirculation valve by pumping several times.

6. Empty the device (see p. 31).7. Screw the device onto a bottle with demineralized water.8. Repeat steps 2 to 5.

6. Screw the device onto an empty bottle.7. Set the maximum volume.8. Carefully pull off the sealing cap.9. Hold the discharge tube over the

opening of the used bottle.10.Empty the device by pumping several

times.11.Only Varispenser plus: Set the valve

switch to recirculation . Empty the recirculation valve by pumping several times.

12.Attach the sealing cap.13.Unscrew the device from the bottle.

The device is now empty.

MaintenanceVarispenser®/Varispenser® plus

English (EN)33

8.2.3 Intensive cleaning

Intensive cleaning is performed directly after standard cleaning. You have to dismantle the device for this.

Prerequisites• The device has been cleaned according to the standard cleaning procedure.

1. Pull off the telescopic aspirating tube and clean it with a soft bottle brush.2. Unscrew the piston mount and carefully pull out the piston.3. Clean the piston and cylinder with a soft bottle brush and water.4. Insert the piston into the cylinder.5. Screw the piston mount to the cylinder sleeve.6. Flush the device with demineralized water and then empty it (see p. 31).

8.3 Autoclaving the device

You can autoclave the device without dismantling it.

Prerequisites• The device has been cleaned according to the intensive cleaning procedure.

1. Remove the sealing cap.2. Remove the telescopic aspirating tube.3. Set the volume selection slider to 20 % of the nominal volume.4. Pull the piston out as far as it will go.5. Place the device and the telescopic aspirating tube into the autoclave on a cloth.

6. Autoclave at 121 °C, 20 minutes, 2 bar.7. Check whether sterilization was successful.8. Let the device cool down for 2 hours after autoclaving.9. Check all parts for deformations or leaks.

Avoid contact with hot metal surfaces.


8.4 Removing the filling valve

To replace the filling valve, remove it with the universal wrench.

Prerequisites• The device has been cleaned according to the standard cleaning procedure (see p. 32).• Universal wrench

8.5 Installing the filling valve

1. Turn the device over.2. Insert the valve ball.3. Manually screw in the filling valve.4. Tighten the filling valve with the universal wrench.

CAUTION! Personal injury due to contact with reagents.The feeding mechanics, valves, telescopic aspirating tube and discharge tube are filled with reagents.Contact with reagents may be harmful to eyes or skin.

Only dismantle cleaned and decontaminated devices.

The valve ball may fall out when removing the valve.

1. Turn the device over.2. Unscrew the filling valve with the

universal wrench.

Technical dataVarispenser®/Varispenser® plus

English (EN)35

9 Technical data9.1 Ambient conditions

9.2 Errors

Test conditions and test analysis in accordance with ISO 8655-6. Tested with an analytical balance tested by the Board of Weights and Measures.

Ambience Only for use indoors.

Ambient temperature 15 °C – 40 °C

Relative humidity 10 % – 90 %, non-condensing.

Atmospheric pressure 700 hPa – 1060 hPa

Model Increment Testing volume Error limits Eppendorf AG

Error

Systematic error Random error

mL mL mL ± % ± mL ± % ± mL

0,5 – 2,5 0.05 0.25 6.0 0.015 1.0 0.0025

0.05 1,25 1.2 0.015 0.2 0.0025

0.05 2,50 0.6 0.015 0.1 0.0025

1 – 5 0,10 1,00 2.5 0.025 0.5 0,0050

0,10 2,50 1.0 0.025 0.2 0,0050

0,10 5,00 0.5 0.025 0.1 0,0050

2 – 10 0.20 2.00 2.5 0,050 0.5 0,0100

0.20 5,00 1.0 0,050 0.2 0,0100

0.20 10,00 0.5 0,050 0.1 0,0100

5 – 25 0,50 5,00 2.5 0,125 0.5 0,0250

0,50 12,50 1.0 0,125 0.2 0,0250

0,50 25,00 0.5 0,125 0.1 0,0250

10 – 50 1,00 10,00 2.5 0,250 0.5 0,0500

1,00 25,00 1.0 0,250 0.2 0,0500

1,00 50,00 0.5 0,250 0.1 0,0500

20 – 100 2.00 20,00 2.5 0,500 0.5 0,1000

2.00 50,00 1.0 0,500 0.2 0,1000

2.00 100,00 0.5 0,500 0.1 0,1000

36Technical dataVarispenser®/Varispenser® plusEnglish (EN)

The errors were determined under the following conditions:

Liquid Demineralized water according to ISO 3696

Number of measurements 10

Ambient temperature 20 °C, constant

Ordering informationVarispenser®/Varispenser® plus

English (EN)37

10 Ordering information10.1 Devices



Description

VarispenserBottle top dispenser with telescopic aspirating tube, universal wrench, and 3 adapters

4960 000.019 4960000019 0,5 mL - 2,5 mL; for bottle thread 32 mm; adapter 28 mm, 40 mm and 45 mm








Description

Varispenser plusBottle top dispenser with return valve, valve switch, and media-dependant fine adjustment. With telescopic aspirating tube, universal wrench, and 3 adapters

4961 000.012 4961000012 0.5 mL - 2.5 mL; for bottle thread 32 mm; adapter 28 mm, 40 mm and 45 mm






38Ordering informationVarispenser®/Varispenser® plusEnglish (EN)

10.2 Spare parts



Description

Discharge tube for Varispenserwith tube holder and sealing cap

4960 826.022 2.5 mL, 5 mL, 10 mL 4960 827.029 25 mL, 50 mL, 100 mL

Discharge tube for Varispenser pluswith tube holder, sealing cap and valve toggle

4961 826.018 2.5 mL, 5 mL, 10 mL 4961 827.014 25 mL, 50 mL, 100 mL

Filling valvewith valve ball

4960 803.006 4960803006 2,5 mL/5 mL/10 mL4960 804.002 4960804002 25 mL/50 mL/100 mL

Telescopic aspirating tube4960 805.009 4960805009 2.5 mL, 5 mL, 10 mL, 25 mL, 50 mL, 100 mL

Discharge tubespiral, length 80 cm

4960 824.003 4960824003 2,5 mL, 5 mL,10 mL4960 825.000 4960825000 25 mL, 50 mL, 100 mL

Drying tube4960 851.000 4960851000 without drying agent

Ventilation cover4960 810.274 4960810274

Universal wrench4960 800.031 4960800031

Universal adapter4960 800.171 100 mL

Ordering informationVarispenser®/Varispenser® plus

English (EN)39

10.3 Threaded adapter



Description

Bottle thread adapter4960 800.040 4960800040 From GL 32 to GL 25, PP4960 800.139 4960800139 From GL 32 to GL 27, PP4960 800.058 4960800058 From GL 32 to GL 28, PP4960 800.066 4960800066 From GL 32 to GL 38, PP4960 800.074 4960800074 From GL 32 to GL 40, PP4960 800.112 4960800112 From GL 32 to GL 45, PP4960 800.163 4960800163 From GL 38 to GL 32, PP4960 800.120 4960800120 From GL 45 to GL 32, PP4960 800.155 4960800155 From GL 45 to GL 38, PP4960 800.147 4960800147 From GL 45 to S 40 (Buttress thread), PP4960 835.005 4960835005 From GL 32 to GL 28, ETFE4960 836.001 4960836001 From GL 32 to GL 38, ETFE4960 833.002 4960833002 From GL 32 to GL 40, ETFE4960 837.008 4960837008 From GL 32 to GL 45, ETFE4960 839.000 4960839000 From GL 45 to GL 38, ETFE4960 838.004 4960838004 From GL 38 to GL 32, ETFE4960 834.009 4960834009 From GL 45 to GL 40, PTFE4960 800.082 4960800082 From GL 32 to NS 19/26, PP4960 800.090 4960800090 From GL 32 to NS 24/29, PP4960 800.104 4960800104 From GL 32 to NS 29/32, PP

Thread adapterfor 5 L-jerrycan

4960 832.006 4960832006 from 45 mm to 17/8" thread

40Transport, storage and disposalVarispenser®/Varispenser® plusEnglish (EN)

11 Transport, storage and disposal11.1 Transport

Tab. 11-1: Transport conditions

11.2 Storage

Tab. 11-2: Storage conditions

11.3 Disposal

Observe the relevant legal regulations for disposing of the product.

NOTICE! Damage as a result of incorrect packing.Eppendorf AG is not liable for damage caused by improper packing.

The device may only be stored and transported in its original packaging.

Air temperature Relative humidity Atmospheric pressure

General transport –20 °C – 50 °C 10 % – 90 %, non-condensing.

300 hPa – 1060 hPa

Air freight –20 °C – 50 °C 10 % – 90 %, non-condensing.

300 hPa – 1060 hPa

Air temperature Relative humidity Atmospheric pressure

Storage –20 °C – 50 °C 10 % – 90 %, non-condensing.

300 hPa – 1060 hPa

Measurement reportVarispenser®/Varispenser® plus

English (EN)41

12 Measurement report

42Measurement reportVarispenser®/Varispenser® plusEnglish (EN)

Evaluate Your Manual

Give us your feedback.www.eppendorf.com/manualfeedback

Your local distributor: www.eppendorf.com/contactEppendorf AG · 22331 Hamburg · [email protected] · www.eppendorf.com

Varispenser plus - Eppendorf · The Varispenser plus has two dispensing settings and one adjustment...

Documents

Transcript of Varispenser plus - Eppendorf · The Varispenser plus has two dispensing settings and one adjustment...