UVStarUVStar®®

Unique Microplates forUnique Microplates for

the UV-Rangethe UV-Range

2

Pre-UVStar®-Period

At my time,

only quartz glass was

available, and it was

fragile

expensive Microplates

hard to clean for reuse

limited in throughput

3

Uniqueness of UVStar®

Microplates with outstanding optical properties

Dedicated for OD reading in the UV-range

Proprietary, unique resin

Optical window down to 200 nm

Available in 96 and 384 well format

4

Prerequisites for UVStar®

Availability of a unique proprietary plastic

Material free of any UV-blocking additives

Production of high quality films (140 µm)

Lamination process free of glue and solvents

Adaptation of µClear®-process to UVStar®

5

µClear®-Technology

Worldwide Patents

Germany: DE 19712484

Europe: Pat. pending

USA: Pat. pending

Direct lamination technology

Process free of glue or solvents

50 - 500 µm films

Enables multi-mode detection

6

Applications of UVStar®-Plates

Optical window down to 200 nm

Background: < 60 mE @ 260 nm

DNA-quantification @ 260 nm

Purity of DNA @ 260/280 nm

Protein concentration @ 280 nm

7

Absorbance of DNA vs. Protein

0

1

2

3

4

240 245 250 255 260 265 270 275 280 285 290

wavelength [nm]

OD

Protein

DNA

8

Clear Bottom Plates

140 µm bottom

1 mm bottom

2 component injection molding bonding of glass sheets

bonding of plastic films

9

Advantages of µClear®-Technology

0

20

40

60

80

100

120

200 250 300 350 400 450 500

wavelength [nm]

tra

ns

mit

tan

ce

[%

]

µClear®

PS 150 µm

PS 1 mm

10

From µClear® to UVStar®

0

20

40

60

80

100

120

200 250 300 350 400 450 500

wavelength [nm]

tra

ns

mit

tan

ce

[%

]

UVStar®

UV-resin #2

µClear®

UV-resin #1

11

Absorbance of UV-transparent ResinsGreiner vs. Competitors

0,0

0,4

0,8

1,2

1,6

2,0

200 220 240 260 280 300

wavelength [nm]

OD

Greiner: 140 µm

Competitor A: 100 µm

Competitor C: 190 µm

12

UV-Light Absorbance384 well µClear® vs. UVStar®

0,0

0,4

0,8

1,2

1,6

2,0

2,4

2,8

200 250 300 350 400 450 500

wavelength [nm]

ab

so

rba

nc

e [

E]

UVStar®

µClear®

13

Measurement of DNA-Conc.96 vs. 384 Well UVStar®-Plates

R2 = 0,9931

R2 = 0,9992

0,0

0,4

0,8

1,2

1,6

2,0

0 20 40 60 80 100 120

conc. DNA [µg/mL]

ab

so

rba

nc

e [

E]

96 well

384 well

14

Cell Growth (WST)in TC-treated 384 Well UVStar®-Plates

0

1

2

3

4

1 2 3 4

days post seeding

ab

so

rba

nc

e [

E]

C127

L929

Exponentiell(L929)Exponentiell(C127)

15

Growth of CHO-Cellsin TC-treated 96 Well Plates

0

2.000

4.000

6.000

8.000

10.000

1 2 3 4 7

days post seeding

flu

ore

sc

en

ce

[R

FU

]

UVStar®

CELLSTAR®

16

Homogeneity of 384 Well Plates

0,000

0,020

0,040

0,060

0,080

0,100

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

column

ab

so

rba

nc

e [

E]

232 nm 280 nm

260 nm 340 nm

17

Performance of Background384 Well UVStar® @ 260 nm

0,000

0,010

0,020

0,030

0,040

0,050

0,060

ab

so

rba

nc

e [

E]

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

A

D

G

J

M

P

column

18

Drift and BackgroundGreiner vs. Competitor

0,049

0,050

0,051

0,052

0,053

0,054

0,055

1 2 3 4 5 6 7 8 9 10 11 12Row

OD

@ 2

60

nm

Competitor C

UVStar®

19

Drift and BackgroundGreiner vs. Competitor

Mean 0,0524

Standard Deviation 0,002

Correlation of Variation 4 %

Competitor Greiner

Mean 0,0498

Standard Deviation 0,001

Correlation of Variation 2 %

1 2 3 4 5 6 7 8 9 10 11 12R1

R4

R70,046

0,050

0,054

0,058

0,0620,0580-0,0620

0,0540-0,0580

0,0500-0,0540

0,0460-0,0500

1 2 3 4 5 6 7 8 9 10 11 12R1

R4

R70,046

0,050

0,054

0,058

0,062 0,0580-0,0620

0,0540-0,0580

0,0500-0,0540

0,0460-0,0500

20

Pathlength & Lambert Beer’s Law I = Io ·e-k’ · c · x

Well pathlength [x]:

not defined

Cuvette pathlength [x]:

fixed

X

IoIo

I

X

I

21

Correlation Pathlength vs. Absorption @ 260 nm

R2 = 0,9231

0

0,02

0,04

0,06

0,08

0,1

0,12

1 2 3 4 5 6 7 8 9 10 11Pathlength [mm]

Pathlenght [mm] OD

0.8 mm 0,0059

2.0 mm 0,0200

4.0 mm 0,0516

6.0 mm 0,0578

8.0 mm 0,095

10.0 mm 0,0828

Quartz Cuvette

[10 mm] = 0,08 OD

22

Chemical and Thermal Resistance

Chemical / Temperature UVStar®

+ 40oC / - 80oC resistant

> 40oC non-resistant

DMSO resistant

Ethanol 96 % resistant

Methanol resistant

HCl (36 %) resistant

H2SO4 (40 %) resistant

Pentane non-resistant

Heptane non-resistant

Benzene non-resistant

23

Benefits of UVStar®-Plates

Available in a 96 well and 384 well format

Extended optical window from 200 to 1000 nm

Background less than 0.060 E @ 260 nm

Minimized variation from well to well

Ideal for quantifying DNA and total protein

No more need for expensive/fragile quartz plates

UVStarUVStar®®Made by