UTILIZATION OF YEAST METABOLITES FOR...
Transcript of UTILIZATION OF YEAST METABOLITES FOR...
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UTILIZATION OF YEAST METABOLITES FOR BIOATTRACTION OF
COCKROACH
OMOREGBEE AIGBOSIOMWAN
A dissertation submitted in partial fulfillment of the
requirements for the award of the degree of
Master of Science (Biotechnology)
Faculty of Biosciences and Bioengineering
Universiti Teknologi Malaysia
JANUARY 2013
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ACKNOWLEDGEMENTS
In preparing this thesis, I was in contact with many people of different fields.
Without their help, I believe this project would not have been the same as presented
here.
First and foremost, I wish to seize this opportunity to express my sincere
appreciation to my thesis supervisor, Dr. Chan Giek Far for her guidance,
encouragement, advice, time and a wealth of new knowledge that will be invaluable
to my future endeavors.
I wish to also thank my research colleagues (Fahmi and Hasima), the
Management and Staff of the Microbiology and Instrument Laboratories, Shelly
Restaurant (FAB Meranti), Kolej Tun Ghafar Baba and Kolej Dato’ Onn Jaafar
residential halls for their wonderful support.
Last but by no means the least, I wish to thank my beloved family for
standing by my side both in rain and shine.
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ABSTRACT
Cockroaches are among the most persistent pests that thrive in protected
locations all over the world. Cockroach control using insecticides and other
chemicals are not desirable because they are toxic to organisms and the environment.
Pests can develop resistance to the chemicals, and chemical raw materials are from
unsustainable source. This study was aimed to identify the novel potential of locally
isolated yeasts, namely Pichia kudriavzevii M12 and Candida ethanolica M2 in
cockroach attraction. The yeasts were fermented in potato dextrose broth (PDB) up
to a week and tested for bioattraction of cockroaches at six locations at the student
residential halls at Universiti Teknologi Malaysia (Johor, Malaysia). Either the 1-day
or 4-day fermented broth from the yeasts were placed as the baits on sticky trap
overnight in order to attract cockroaches. Freeze drying on the fermented broth was
carried out and the resultant powder was sprinkled on the cockroach trap and tested
for cockroach attraction. PDB was used as control. The 4-day fermented PDB of P.
kudriavzevii M12 was found to be the better cockroach attractant, which trapped the
highest number of both nymphs and adult cockroaches (an average of 48
cockroaches per catch). Ssuccessive attraction was done consecutively at the same
location after two weeks, which resulted in a decrease of almost 80% of the
cockroach population at the studied location. The metabolites in the 4-day fermented
PDB of P. kudriavzevii M12 were profiled by liquid chromatography-mass
spectrometry (LC-MS/MS), thus revealing the presence of secondary metabolites
from the yeast strain for cockroach attraction. A total of 44 exometabolites with
diverse properties and structures were identified and many were intermediates and
products of the central metabolic pathway such as lipids, carboxylic acids and esters.
In conclusion, P. kudriavzevii M12 showed a great potential as an eco-friendly
cockroach attractant and the attraction could be as a result of the metabolites
produced.
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ABSTRAK
Lipas adalah antara perosak yang paling sering ditemui di pelbagai lokasi
yang dilindungi di seluruh dunia. Kawalan terhadap lipas menggunakan racun
serangga dan bahan kimia lain tidaklah diterima disebabkan oleh ketoksikan kepada
organisma dan persekitaran. Perosak boleh membangunkan rintangan kepada bahan
kimia, dan bahan-bahan mentah kimia adalah dari sumber yang tidak mapan. Kajian
ini bertujuan untuk mengenal pasti potensi baru yis tempatan terpencil, iaitu Pichia
kudriavzevii M12 dan Candida ethanolica M2 sebagai tarikan lipas. Yis telah ditapai
dalam potato dextrose broth (PDB) sehingga seminggu dan diuji untuk tarikan lipas
di enam lokasi di kediaman pelajar di Universiti Teknologi Malaysia (Johor,
Malaysia). Sampel yang telah ditapai selama 1 hari dan 4 hari diletakkan sebagai
umpan perangkap yang lekit untuk menarik lipas selama semalaman. Kering beku
sampel yang ditapai telah dijalankan dan serbuk yang terhasil dipercikkan pada
perangkap lipas dan diuji untuk tarikan lipas. Eksperimen kawalan d ijalankan
menggunakan PDB sahaja. Sampel yang ditapai selama 4 hari oleh P. kudriavzevii
M12 didapati menjadi umpan lipas yang lebih baik, yang berjaya memerangkap
bilangan tertinggi anak dan lipas dewasa (purata 48 lipas setiap tangkapan). Tarikan
dilakukan berturutan di lokasi yang sama selepas dua minggu, yang mengakibatkan
pengurangan populasi lipas sebanyak hampir 80% di lokasi yang dikaji. Metabolit
dalam sampel PDB yang ditapai pada hari ke-4 oleh P. kudriavzevii M12 telah
dikajikan menggunakan kromatografi cecair-spektrometri jisim (LC-MS/MS)
mendedahkan bahawa terdapatnya metabolit sekunder dari yis. Sebanyak 44
metabolit dengan ciri-ciri dan struktur yang pelbagai telah dikenalpasti dan
sebahagiannya adalah perantara dan produk laluan metabolik pusat seperti lemak,
asid karbosilik dan ester. Kesimpulannya, P. kudriavzevii M12 menunjukkan potensi
yang besar sebagai umpan lipas yang mesra alam dan tarikan adalah disebabkan oleh
metabolit yang dihasilkan.
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TABLE OF CONTENTS
CHAPTER TITLE PAGE
TITLE i
SUPERVISOR’S DECLARATION ii
DECLARATION iii
DEDICATION iv
ACKNOWLEDGEMENTS v
ABSTRACT vi
ABSTRAK vii
TABLE OF CONTENTS viii
LIST OF TABLES xii
LIST OF FIGURES xiii
LIST OF ABBREVIATIONS xiv
LIST OF APPENDICES xv
1 INTRODUCTION
1.1 Introduction 1
1.2 Statement of the Problem 6
1.3 Objectives of Research 7
1.4 Scope of Research 7
1.5 Significance of Research 7
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2 LITERATURE REVIEW
2.1 Metabolites 8
2.2 Volatile Metabolites 9
2.3 Importance of Volatile Metabolites 10
2.3.1 Flavors and Fragrances 11
2.3.2 Identification, profiling and
chemotaxonomic studies
11
2.3.3 Communication 11
2.3.4 Inhibitory Functions 12
2.3.5 Detection of Contamination 12
2.3.6 Attraction 12
2.4 Sources of Volatile Metabolites 13
2.5 Volatile Metabolites from Microorganisms 14
2.6 Yeast Fermentation 17
2.7 Attraction and Traps 18
2.8 The Saccharomycetaceae Family 20
2.8.1 Candida ethanolica 21
2.8.2 Pichia kudriavzevii 21
2.9 Media in Volatile Metabolites Production by
Yeasts
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2.10 Cockroach as Pest 23
2.11 Cockroach Control 24
2.12 Yeast Volatile Metabolites as Attractants 25
2.13 Identification of Volatile Metabolites from Yeasts
Fermentation
25
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2.14 LC-MS/MS 26
2.15 Downstream Processing of Fermented Broth 28
2.16 Freeze Drying 29
3 MATERIALS AND METHODS
3.1 Experimental Design 31
3.2 Materials and Equipment 31
3.3 Yeasts Source 33
3.4 Media Preparation 33
3.5 Yeast Maintenance and Subculturing 33
3.6 Production of Volatile Metabolites 34
3.7 Identification of Volatile Metabolites by LC-
MS/MS
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3.8 Cockroach Attraction using Yeast Metabolites 35
3.9 Cockroach Attraction using Freeze-Dried
Metabolites
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4 RESULTS AND DISCUSSION
4.1 Bioattraction 37
4.2 Profiling of Exometabolites from P. kudriavzevii
M12
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5 CONCLUSIONS AND RECOMMENDATION
5.1 Conclusions 81
5.2 Recommendation 81
REFERENCES 83
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APPENDICES
APPENDIX A 108
APPENDIX B 109
APPENDIX C 110
APPENDIX D 111
APPENDIX E 112
APPENDIX F 113
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LIST OF TABLES
TABLE NO. TITLE PAGE
2.1 Some volatile metabolites showing variation in
physical, chemical characteristics as well as source
and biodiversity activity
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4.1 Mass spectral profiling of exometabolites found in
the 4-day fermented PDB of P. kudriavzevii M12
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4.2 Characteristics of exometabolites found in the 4-day
PDB fermented of P. kudriavzevii M12
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LIST OF FIGURES
FIGURE
NO.
TITLE PAGE
2.1 Pathway for volatile metabolites 15
2.2 Terms used for semiochemicals 19
3.1 Flowchart of the experimental procedure for the bioattraction of
cockroaches
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4.1 Bioattraction of domestic cockroaches to P. kudriavzevii M12
and C. ethanolica M2
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4.2 Successive tests of cockroach attraction to the 4-day fermented
broth of P. kudriavzevii M12 at the same location
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4.3 Bioattraction of domestic cockroaches to P. kudriavzevii M12 (a)
4-day fermented broth, and (b) freeze dried 4-day
fermentated broth
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4.4
4.5
Optical density at the end of fermentation
Ratio of the exometabolites (n = 44) identified from LC-MS/MS
profiling of the 4-day fermented PDB of P. kudriavzevii M12
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42
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LIST OF SYMBOLS/ABBREVIATIONS/NOTATIONS/TERMINOLOGY
Bla g 1 - Cockroach allergen
Bla g 2 - Cockroach allergen
bp - Boiling point
oC - Degrees Celsius
DNA - Deoxyribonucleic acid
ESI - Electron Spray Ionization
g - gram
GC - Gas chromatography
HPLC - High performance liquid chromatography
LC - Liquid chromatography
LC-MS/MS - Liquid chromatography tandem mass spectrometry
MALDI - Matrix assisted laser desorption/ionization
mb - Milli bar
mmHg - Millimeters of mercury
MS - Mass spectroscopy
nm - nanometer
OD - Optical density
PDA - Potato dextrose agar
PDB - Potato dextrose broth
rpm - Revolutions per minute
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LIST OF APPENDICES
APPENDIX TITLE PAGE
A Mass spectra for positive ion detection of one day
fermented PDB of P.kudriavzevii M12.
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B Mass spectra for positive ion detection of four days
fermented PDB of P.kudriavzevii M12.
109
C Mass spectra for positive ion detection of PDB. 110
D Mass spectra for negative ion detection of one day
fermented PDB of P.kudriavzevii M12.
111
E Mass spectra for negative ion detection of four days
fermented PDB of P.kudriavzevii M12.
112
F Mass spectra for negative ion detection of PDB 113
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CHAPTER 1
INTRODUCTION
1.1 Introduction
Cockroaches (order: Blatteria) are among the most persistent pests that thrive
in protected locations all over the world. American, brown-banded, German and
Oriental cockroaches are the four most common species in human habitats (Hahn and
Ascerno, 2005). They can be found in boiler rooms, heated steam tunnels, floor
drains, water heaters, bath tubs (Eggleston and Arruda, 2001), kitchen sink, wall
cracks, underneath or inside cupboards, behind drawers, around pipes or conduits,
behind window or door frames, in radio and TV cabinets (Koehler et al., 2007).
Cockroaches are objectionable because they compete with humans for food,
carry allergens in saliva, fecal material, secretions, cast skins, debris and dead bodies
that can lead to asthma in humans (Eggleston and Arruda, 2001), harbor pathogenic
bacteria, contaminate paper and fabrics, and impart repulsive stains and odours
(Koehler et al., 2007) .
The life cycle of cockroach consists of egg, nymph and adult and they can
live for one year. Nymphs are more than adults in natural populations (Hahn and
Ascerno, 2005). Cockroaches are nocturnal and omnivorous and will eat anything
though they can stay for about one month without food and for about two weeks
without water. Cockroach population in an enclosure is governed by presence of food
and water, height of location (for some species), humidity, temperature darkness
(Eggleston and Arruda, 2001) and history of control measures (Wang and Bennett,
2006).
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Trapping, mating distruptions, attracticides are used in cockroach control.
Organophosphates, carbamates, pyrethrins, chlorinated hydrocarbons and inorganics
are some of the pesticides used to control cockroaches (Eggleston and Arruda, 2001).
Traps are relatively more effective in controlling cockroach (Rust et al., 1999).
Attractiveness of methylcyclohexyl n-alkanoates to the German cockroach was
reported by Iida and co-workers (Iida et al., 1981). Many chemical attractants used in
commercial cockroach traps are injurious to humans and the environment (Quesada
et al., 2004). Pest resistance, toxicity and non-sustainable raw materials of pesticides
are some of the factors behind the trend to replace chemical feedstock, processes and
end products with bio-based alternatives.
Cockroaches emit pheromones for communication and other activities. Three
semiochemicals from stale beer and peanut butter are capable of being used as
cockroach bioattractants were identified by Karimifar and co-workers (Karimifar et
al., 2011). Foods such as bread, peanut butter, molasses and beer can act as
cockroach attractants. Food baits are not used in commercial pest control strategies
as they often turn out to be breeding grounds for pests (Cox and Collins, 2002).
Many insects respond to chemicals released by same species or chemicals released
by other organisms. Many plants release volatile chemicals that are able to affect
other organisms such as insects. Semiochemicals are pheromones and
allelochemicals, many of which are responsible for insect behaviors such as feeding
and reproduction. They are mostly volatile metabolites that are involved in signaling
and other functions (Maffei, 2010).
Metabolites are involved in metabolism either as intermediates or as the final
products. Metabolism can be divided into anabolism in which simple molecules are
used to build large, complex molecules and catabolism in which complex, large
molecules are broken into simple molecules. Most of the time, each metabolite can
serve more than one purpose (Kooijman and Segel, 2005). Most metabolites are
within cells where they are utilized by enzymes in various biochemical reactions.
Some of the metabolites have control functions. Metabolites include lysine,
riboflavin (Damain, 1980), ethanol, acetic acid (Styger et al., 2011). Two subgroups
of metabolites are primary and secondary metabolites. Primary metabolites have
physiological functions (Gika et al, 2012). Secondary metabolites serve ecological
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functions (Demain, 1998). Primary pathways normally yield few products but are
present in many organisms. Secondary metabolic pathways yield many products but
are present in few organisms. Antibiotics make up most of the 50 most important
secondary metabolites (Demain, 1999).
Volatile metabolites are intermediates and final products of metabolism. A
major feature of volatile metabolites is the ease of evaporation at normal temperature
and pressure. They act over a wide range of distances, concentration and
environment (Humphris et al., 2002). Yeasts produce volatile metabolites which can
be grouped into alcohols, acids, esters, ketones and phenols. Such volatiles include
ethanol, 3-methyl-1-butanol, 3- hydroxyl-2-butanone, ethyl propanoate, 2-phenyl-
ethanol (Nout and Bartelt, 1998). Volatile metabolites are useful to both the
organism producing them and other organisms. Volatiles are useful as flavors,
fragrances (Berger, 2009), inhibitors (Ting et al., 2010), and attractants (Rowan,
2011), as well as for communication (Maffei, 2010), identification and profiling
(Frisvad et al., 2007), and detection of contaminants (Berge et al., 2011). Volatile
metabolites are produced by plants, animals, microorganisms and through chemical
synthesis. Yields from plants and animals are low compared to microbial sources
(Vandamme, 2003). Plant cell cultures are not economically viable for producing
volatile metabolites (Verpoorte et al., 2002). Chemical (synthetic) volatile
metabolites are relatively cheap with large market share but disadvantaged by
perceived environmental toxicity and possible formation of recemic mixtures (Longo
and Sanroman, 2006). Major advantages of microbial based production of volatile
metabolites include low energy input, reduced emission of pollutants, easy
purification and renewable raw materials (Chelmer et al., 2006). Some volatile
metabolites are produced through microbial fermentation of various substrates.
Microbial volatile production depends on medium, species (Borjesson et al., 1992),
temperature, pH, initial aeration (Reddy and Reddy, 2011), culture age and volatiles
from other microorganisms (Tirranen and Gitelson, 2006).
Yeasts are the most suitable source for microbial volatile production as they
tolerate high sugar concentration, anaerobic growth, high salt concentration,
resistance to inhibitors in the biomass (Nevoigt, 2008). An alcoholic fermentation
takes place in the absence of oxygen and presence of yeasts, nutrient medium,
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suitable pH and temperature. Alcohols, vitamins, hormones, antibiotics, enzymes,
acids are made through fermentation. Yeast utilization of glucose can be through the
fermentation of glucose, oxidation of glucose or oxidation of ethanol.
Saccharomyces cerevisiae can completely ferment glucose, fructose, galactose,
sucrose and maltose (Yoon et al., 2003). Volatile metabolites are produced during
bacterial and yeast fermentations but in an experiment, only volatile metabolites
from yeast fermentation were able to attract insects (Nout and Bartelt, 1998).
The Saccharomycetaceae family has about 20 genera, which include
Saccharomyces, Candida and Pichia. Members of the family reproduce by budding
and live in environments rich in carbohydrates. Saccharomyces cerevisiae is the most
important yeast as a model eukaryotic organism. Saccharomyces cerevisiae is used in
baking, brewing and wine making. Saccharomyces cerevisiae and closely related
yeasts dominate alcoholic fermentations as they are tolerant of high alcohol content
and they also use the produced ethanol as feedstock (Piskur et al., 2006).
Candida ethanolica was first isolated from industrial fodder yeast and can
grow on ethanol as the only carbon source. C. ethanolica is one of the yeasts
involved in shubat (a fermented camel milk) production (Shori, 2012), Ghanaian
heaped cocoa bean fermentation (Daniel et al., 2009), mescal (Mexican distilled
beverage) (Valdez, 2011), sour cassava starch fermentation (Lacerda et al., 2005).
Pichia kudriavzevii is synonymous with Issatchenkia orientalis based on
molecular, biochemical and phenotypic characterization (Bhadra et al., 2007). Pichia
kudriavzevii was shown to degrade phytate in ‘togwa’, a cereal food in Tanzania
(Hellstrom et al., 2012), produce ethanol from alkali- treated rice straw (Oberoi et al.,
2012), ferment cotton stalks in a combined saccharification and fermentation process
for ethanol production (Kaur et al., 2012), part of fen-daqu, starter culture for
Chinese liquor production (Zheng et al., 2012), among the yeasts involved in
‘tchapalo’, a local beer in Cote d’ Ivoire (N’guessan et al., 2011) and among the
yeasts involved in Ghanaian heap cocoa bean fermentation (Daniel et al., 2009).
Media composition is important in yeast fermentation performance (Hahn-
Hagerdal et al., 2005). Agar plates and nutrient broth are used in most yeast
fermentations. Among the common media for yeast fermentation are wort (Lodolo et
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al., 2008), molasses (Alegre et al., 2003) and potato dextrose broth (Kalyani et al.,
2000). Yeast stock cultures are normally kept at 4oC on potato dextrose agar
(Lakshmanan and Radha, 2012). Most yeast utilize hexose sugars (monosaccharides
and disaccharides). The media used can improve yield, increase biomass formation
and decrease fermentation period (Chunkeng et al., 2011). The ecology and profile of
microorganisms during fermentation could be affected by the media used (Hsieh et
al., 2012). Potato dextrose broth is commonly used for the cultivation of yeasts and
molds. Its low pH discourages bacterial growth (Condolab, 2012).
Chromatography is used to separate complex mixtures but it cannot readily
identify the constituents of separated unknown mixtures. It is always desirable to
couple separation processes to identification steps. Liquid chromatography (LC) and
gas chromatography (GC) are two of the leading chromatographic methods. LC is
favoured over GC for samples susceptible to degradation as LC does not involve
derivation step. In order to identify, LC is normally coupled to mass spectrometry
(MS) that is in tandem. Liquid chromatography – tandem mass spectrometry (LC-
MS/MS) was found to have lower limit of quantification, less costly to run, reduced
preparation time, does not involve derivation of sample, and reduced run time (Coles
et al., 2007). LC-MS/MS is best suited for the analysis of chemically and thermally
unstable compounds (Johnson, 2005).
Downstream processing involves the recovery of the desired product from the
fermentation broth. Removal of insolubles, product isolation, product purification
and product polishing are the major groupings of the unit operations of fermentation
products recovery. Low product concentration makes product recovery expensive
(Schugerl, 2000).
Freeze drying (lyophilization) is a dehydration process in which solvent is
removed through sublimation. It is used for the recovery of heat sensitive products
such as volatile metabolites. Compared to air drying, the retention of volatile
materials was more in the freeze dried sample (Krokida and Philippoules, 2006).
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1.2 Statement of Problem
Cockroach control using insecticides and other chemicals are not desirable
because they are toxic to organisms and the environment (Quesada et al., 2004).
Pests can develop resistance to the chemicals. In addition, chemicals are
unsustainable as the raw materials of the chemicals are from petroleum. Hence, there
is consumer demand for natural product based compounds.
On the other hand, foods as attractants favour all organisms thereby providing
breeding grounds (Cox and Collins, 2002). Foods have limited range of effectiveness
and are non-specific.
Biological based attractants from plants and animals are very low in yields
(Vandamme, 2003). Extraction from plants is very difficult, depends on the season
and some of the exotic plants have lost the ability to produce desired volatile
metabolites (Dudareva and Pichersky, 2000).
Microorganisms produce volatile metabolites that can potentially act as insect
attractants. Volatile metabolites from yeasts have been found to be better at attracting
insects than volatile metabolites from bacteria (Nout and Bartelt, 1998).
Therefore, there is the need to test if volatile metabolites from yeast
fermentation can attract cockroaches and the leading volatile metabolites responsible
for the attraction. In this study, two locally isolated yeasts, namely Candida
ethanolica M2 and Pichia kudriavzevii M12 were tested for their potential in
bioattraction of domestic cockroaches and production of exometabolites.
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1.3 Objectives of the Research
The specific objectives of this study were:
1. To determine the potential of yeast strains C. ethanolica M2 and P.
kudriavzevii M12 in producing metabolites for bioattraction of domestic
cockroaches
2. To compare the fresh and freeze-dried yeast culture supernatants in its
efficiency in bioattraction of domestic cockroaches
3. To study the exometabolites of P. kudriavzevii M12
1.4 Scope of Research
This research work focused on the production of volatile metabolites from
yeast strains C. ethanolica M2 and P. kudriavzevii M12 which were cultured in
potato dextrose broth (PDB) up to a period of a week. The culture supernatant
containing volatile metabolites were tested as bait of commercial trap for the
attraction of domestic cockroaches at six different locations at the student residential
halls in Universiti Teknologi Malaysia. Freeze-drying of the culture supernatant was
attempted as a preservation technique and the efficiency of the freeze-dried
metabolites in bioattraction of cockroach was determined. As the fermentation of P.
kudriavzevii M12 was found to be significant, LC-MS/MS profiling was performed
to determine the leading metabolites in the supernatant from the four-days
fermentation broth.
1.5 Significance of Research
This research is significant as it has the potential to discover non-toxic,
natural bait from yeasts which may replace the harmful chemical b aits in domestic
cockroach traps. In the future, the identified metabolites could be optimized for the
production of cheap bait for domestic cockroach attraction.
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REFERENCES
Abbas, K. A. (2010). The significance of glass transition temperature in processing
of selected fried food products: A review. Modern Applied Science, 4(5), 3-
21.
Abraham, B. G., and Berger, R. G. (1994). Higher fungi for generating aroma
components through novel biotechnologies. Journal of Agricultural and
Food Chemistry 42, 2344-2348.
Alegre, R. M., Rigo, M., and Joekes, I. (2003). Ethanol fermentation of a diluted
molasses medium by Saccharomyces cerevisiae immobilized on chrysotile.
Brazilian Archives of Biology and Technology, 46(4), 751-757.
Appling, D. R. (1991). Compartmentation of folate-mediated one-carbon metabolism
in eukaryotes. The FASEB Journal, 5, 2645-2651.
Arranz, S., Chiva-Blanch, G., Valderas-Marinez, P., Medina-Remon, A., Lamuela-
Raventos, R. M., and Estruch, R. (2012). Wine, beer, alcohol and
polyphenols on cardiovascular disease and cancer. Nutrient, 4, 759-781.
Arruda, L. K., Vailes, L. D., Ferriani, V. P. L., Santos, A. B. R., Pomes, A., and
Chapman, M. D. (2001). Cockroach allergen and asthma. Journal of Allergy
and Clinical Immunology, 107, 419-428.
Bai, F. W., Anderson, W. A., and Moo-Young, M. (2008). Ethanol fermentation
technologies from sugar and starch feedstocks. Biotechnology Advances, 26,
89-105.
Bajad, S. U., Lu, W., Kimball, E. H., Yuan, J., Peterson, C., and Rabinowitz, J. D.
84
(2006). Separation of quantification of water soluble cellular metabolites by
hydrophilic interaction chromatography- tandem mass spectrometry. Journal
of Chromatography A, 1125, 76-88.
Batra, S. W. T. (1985). Polyester-making bees and other innovative insect chemists.
Journal of Chemical Education, 62(2), 121-124.
Baudot, A., and Marin, M. (1996). Dairy aroma compounds recovery by
pervaporation. Journal of Membrane Science, 120, 207-220.
Berge, P., Ratel, J., Fournier, A., Jondreville, C., Feidt, C., Roudaut, B., Le Bizec,
B., and Engel, E. (2011). Use of volatile compound metabolic signatures in
poultry liver to back-trace dietary exposure to rapidly metabolized
xenobiotics. Environmental Science and Technology, 45, 6584-6591.
Berger, R. G. (2009). Biotechnology of flavours – the next generation.
Biotechnology Letters, 31,1651-1659.
Bhadra, B., Rao, R. S., Kumar, N. N., Chaturvedi, P., and Sarkar, P. K. (2007).
Pichia cecembensis sp. nov. isolated from a papaya fruit (Carica papaya L.,
Caricaceae). FEMS Yeast Research, 7(4), 579-584.
Bhandari, B. R., and Howes, T. (1999). Implication of glass transition for the drying
and stability of dried foods. Journal of Food Eengineering, 40, 71-79.
Bi, P. Y., Dong, H. R., and Guo, Q. Z. (2009). Separation and purification of
Penicillin G from fermentation broth by solvent sublation. Separation and
Purification Technology, 65, 228-231.
Bianchi, F., Careri, M., Mangia, A., Mattarozzi, M., Musci, M., Concina, I., and
Gobbi, E. (2010). Characterisation of the volatile profile of orange juice
contaminated with Alicyclobacillus acidoterrestris. Food Chemistry, 123,
653-658.
Billheimer, J. T., Avart, S., and Milani, B. (1983). Separation of steryl esters by
reverse-phase liquid chromatography. Journal of Lipid Research, 24,1646-
1651.
85
Boch, R., Shearer, D. A., and Stone, B. C. (1962). Identification of isoamyl acetate
as an active component of the sting pheromone of the honey bee. Nature,
195, 1018-1020.
Bode, R., and Birnbaum, D. (1987). D-amino acid oxidase, aromatic L-amino amino-
transferase, and aromatic lactate dehydrogenase from several yeast
species: Comparison of enzyme activities and enzyme specificities.
Acta Biotechnology, 7(3), 221-225.
Boldyrev, A. A., Stvolinsky, S. L., Fedorova, T. N., and Suslina, Z. A. (2010).
Carnosine as a natural antioxidant and geroprotector: from molecular
mechanisms to clinical trials. Rejuvenation Research, 3(2-3), 156-158.
Borjesson, T., Stollman, U., and Schnurer, J. (1990). Volatile metabolites and other
indicators of Penicillium aurantiogriseum growth on different substrates.
Applied and Environmental Microbiology, 56(12), 3705-3710.
Borjesson, T., Stollman, U., and Schnurer, J. (1992). Volatile metabolites produced
by six fungal species compared with other indicators of fungal growth on
cereal grains. Applied and Environmental Microbiology, 58(8), 2599-2605.
Botella, C., Diaz, A. B., Wang, R., Koutines, A., and Webb, C. (2009). Particulate
bioprocessing: A novel process strategy for biorefineries. Process
Biochemistry, 44, 546-555.
Breitling, R., Pitt, A. R., and Barrett, M. P. (2006). Precision mapping of the
metabolome. Trends in Biotechnology, 24(12), 543-548.
Brindley, D. N., English, D., Pilquil, C., Buri, K. and Ling, Z. (2002). Lipid
phosphate phosphatases regulate signal transduction through glycerolipids.
Biochimica et Biophysica Acta, 1582, 33-44.
Bunch, A. W., and Harris, R. E. (1986). The manipulation of micro-organisms for
the production of secondary metabolites. Biotechnology and Genetic
Engineering Review, 4, 117-144.
86
Cao, L., Zhang, P., and Grant, D. F. (2009). An insect farnesyl phosphatase
homologuous to the N-terminal domain of soluble epoxide hydrolase.
Biochemical and Biophysical Research Communications, 380, 188-192.
Casaregola, S., Weiss, S., and Morel, G. (2011). New perspectives in
hemiascomycetous yeast taxonomy. C. R. Biologies, 334, 590-598.
Cecchini, F., Manzano, M., Manddabi, Y., Perelman, E., and Marks, R. S. (2012).
Chemiluminescent DNA optical fibre sensor for Brettanomyces bruxellensis.
Journal of Biotechnology, 157, 25-30.
Chan, G. F., Gan, H. M., Ling, H. L., and Rashid, N. A. A. (2012). Genome
sequence of Pichia kudriavzevii M12, a potential producer of bioethanol and
phytase. Eukaryotic Cell, 11(10), 1300-1301.
Chemler, J. A., Yan, Y., and Koffas, M. A. G. (2006). Biosynthesis of isoprenoids,
polyunsaturated fatty acids and flavonoids in Saccharomyces cerevisiae.
Microbial Cell Factories, 5(20), 1-9.
Chen, H., Lee, Y., Wei, Y., and Juang, R. (2008). Purification of surfactin in
pretreated ermentation broth by adsorptive removal of impurities.
Biochemical Engineering Journal, 40, 452-459.
Christ (2012). Smart freeze drying. Retrieved December 11, 2012 from
http://www.martinchrist.de/fileadmin/my_uploads/christ/
en/Christ_Theorie_Katalog_en_web.pdf
Christie, W. W., (2012). Phosphatidic acid, lysophosphatidic acid and related lipids:
Structure occurence, biochemistry and analysis. Retrieved December 19,
2012 from http://www. lipidlibrary.aocs.org.
Chunkeng, H., Qing, Q., and Peipei, G. (2011). Medium optimization for improved
ethanol production in very high gravity fermentation. Chinese Journal of
Chemical Engineering, 19(6), 1017-1022.
Cocolin, L., Bisson, L. F., and Mills, D. A. (2000). Direct profiling of the yeast
dynamics in wine fermentations. FEMS Microbiology Letters, 189, 81-87.
87
Coetzee, C., and du Toit, W. J. (2012). A comprehensive review on Sauvignon blanc
aroma with a focus on certain positive volatile thiols. Food Research
International, 45, 287-298.
Coles, R., Kushnir, M. M., Nelson, G. J., McMullin, G. A., and Urry, F. M. (2007).
Simultaneous determination of codeine, morphine, hydrocodone,
hydromorphone, oxycodone, and 6-acetylmorphine in urine, serum, plasma,
whole blood, and meconium by LC-MS/MS. Journal of Analytical
Toxicology, 31,1-14.
Condalab (2012). Potato dextrose broth. Retrieved December 9, 2012 from
www.condalab.com/pdf/1261.pdf
Conner, W. E., Eisner, T., Vander Meer, R. K., Guerrero, A., and Meinwald, J.
(1981). Precopulatory sexual interaction in an arctiid moth (Utetheisa
ornatrix): Role of a pheromone derived from dietary alkaloids. Behavioural
Ecology and Sociobiology, 9, 227-235.
Cottier, F. and Muhlschlegel, F.A. (2012). Communication in fungi. International
Journal of Microbiology. doi:10.1155/2012/351832
Cox, P. D., and Collins, L. E. (2002). Factors affecting the behaviour of bettle pests
in stored grain, with particular reference to the development of lures. Journal
of Stored Products Research, 38, 95-115.
Cramer, S. M., and Holstein, M. A. (2011). Downstream bioprocessing: Recent
advances and future promise. Current Opinion in Chemical Engineering, 1,
27-37.
Damain, A. L. (1980). Microbial production of primary metabolites.
Naturwissenschaften, 67, 582-587.
Damain, A. L. (1998). Induction of microbial secondary metabolism. International
Microbiology, 1, 259-264.
Damain, A. L. (1999). Pharmaceutically active secondary metabolites of
microorganisms. Applied Microbiology and Biotechnology, 52, 455-463.
88
Daniel, H., Vrancken, G., Takrama, J. F., Camu, N., De Vos, P., and De Vuyst, L.
(2009).Yeast diversity of Ghanaian cocoa bean heap fermentation. FEMS
Yeast Research, 9, 774-783.
Dear, G. J., Fraser, I. J., Patel, D. K., Long, J., and Pleasance, S. (1998). Use of
liquid chromatography-tandem mass spectrometry for the quantitative and
qualitative analysis of antipsychotic agent and its metabolites in human
plasma and urine. Journal of Chromatography A, 794, 27-36.
De Lucca, S. D., Taylor, D. J. M., O’Meara, T. J., Jones, A. S., and Tovey, E. R.
(1999). Measurement and characterization of cockroach allergens detected
during normal domestic acivity. Journal of Allergy and Clinical Immunology,
104(3), 672-680.
De Torres, C., Diaz-Maroto, M. C., Hermosin-Gutierrez, I., and Perez-Coello, M. S.
(2010).Effect of freeze-drying and oven drying on volatiles and phenolics
composition of grapeskin. Analytica Chimica Acta, 660, 177-182.
Dickson, R. C., New insights into sphingolipid metabolism and function in budding
yeast.Journal of Lipid Research, 49, 909-921.
Dien, B. S., Cotta, M. A., and Jeffries, T. W. (2003). Bacteria engineered for fuel
ethanol production: Current status. Applied Microbiology and
Biotechnology, 63, 258-266.
Diez, J., Martinez, J. P., Mestres, J., Sasse, F., Frank, R., and Meyerhaus, A. (2012).
Myxobacteria: natural pharmaceutical factories. Microbial Cell Factories, 11
(52), 1-3.
Dinglasan, M. J. A., Ye, Y., Edwards, E. A., and Mabury, S. A. (2004).
Fluorotelomer alcohol biodegradation yields poly- and perflourinated acids.
Environmental Science and Technology, 38, 2857-2864.
Dudareva, N., and Pichersky, E. (2000). Biochemical and molecular genetic aspects
of floral scents. Plant Physiology, 122, 627-633.
89
Dudareva, N., and Pichersky, E. (2008). Metabolic engineering of plant volatiles.
Current Opinion in Biotechnology, 19, 181-189.
Edris, A. E., and Malone, C. R. (2011). Formulation of banana aroma impact ester in
water-based microemulsion nano-delivery system for flavoring applications
using sucrose laurate surfactant. Procedia Food Science, 1, 1821-1827.
Eggleston, P. A., and Arruda, L. K. (2001). Ecology and elimination of cockroaches
and allergens in the home. Journal of Allergy and Clinical Immunology,
107(3), S422-S429.
Ekesi, S., Maniania, N. K., and Lux, S. A. (2003). Effect of soil temperature and
moisture on survival and infectivity of Metarhizium anisopliae to four
tephritid fruit fly puparis. Journal of Invertebrate Pathology, 83, 157-167.
El-Sayed, A. M., Heppelthwaite, V. J., Manning, L. M., Gibb, A. R., and Suckling,
D. M.(2005). Volatile constituents of fermented sugar baits and their
attraction to Lepidopteran species. Journal of Agricultural and Food
Chemistry, 53, 953-958.
Emelyanova, E. V. (2000). Relationship between magnesium and iron uptake by the
yeast Candida ethanolica. Process Biochemistry, 36, 517-523.
Enomoto, H., Ishida, T., Hamagami, A., and Nishida, R. (2010). 3-Oxygenated α-
ionone derivatives as potent male attractants for the solanaceous fruit fly,
bactrocera latifrons (Diptera : Tephritidae), and sequestered metabolites in
the rectal gland. Applied Entomology and Zoology, 45(4), 551-556.
Erten, H., and Tanguler, H. (2010). Influence of Williopsis saturnus yeasts in
combination with Saccharomyces cerevisiae on wine fermentation. Letters in
Applied Microbiology, 50, 474-479.
Figueiredo, A. C., Barroso, J. G., Pedro, L. G., and Scheffer, J. J. C. (2008). Factors
affecting secondary metabolite production in plants: Volatile components
and essential oils. Flavour and Fragrance Journal, 23(4), 213-226.
90
Fitzgerald, R. L., Riveria, J. D., and Herold, D. A. (1999). Broad spectrum drug
identification directly from urine, using liquid chromatography – tandam
mass spectrometry. Clinical Chemistry, 45(8), 1224-1234.
Fleet, G. H. (1990). Yeast in dairy products. Journal of Applied Bacteriology, 68,
199-211.
Flink, J., and Karel, M. (1970). Retention of organic volatiles in freeze-dried
solutions of carbohydrates. Journal of Agricultural and Food Chemistry,
18(2), 295-297.
Florez, A. B., Belloch, C., Alvarez-Martin, P., Querol, A., and Mayo, B. (2010).
Candida cabralensis sp. nov., a yeast species isolated from traditional
Spanish blue-veined Cabrales cheese. International Journal of Systematic
and Evolutionary Microbiology,60, 2671-2674.
Forster, J., Famili, I., Fu, P., Palsson, B., and Nielsen, J. (2003). Genome-scale
reconstruction of the Saccharomyces cerevisiae metabolic network. Genome
Research, 13(2), 244-253.
Frisvad, J. C., Larson, T. O., de Vries, R., Meijer, M., Houbraken, J., Cabanes, F. J.,
Ehrlich, K., and Samson, R. A. (2007). Secondary metabolite profiling,
growth profiles and other tools for species recognition and important
Aspergillus mycotoxins. Studies in Mycology, 59, 31-37.
Fukuda, K., Yamamoto, N., Kiyokawa, Y., Yanagiuchi, T., Wakai, Y., Kikamoto,
K., Inoue,Y., and Kimura, A. (1998). Balance of activities of alcohol
acetyltransferase and esterase in Saccharomyces cerevisiae is important for
production of isoamyl acetate. Applied and Environmental Microbiology,
64(10), 4076-4078.
Fukuda, K., Yamamoto, N., Kiyokawa, Y., Yanagiuchi, T., Wakai, Y., Kikamoto,
K., Inoue, Y., and Kimura, A. (2000). Purification and characterization of
isoamyl acetate-hydrolyzing esterase encoded by the IAH1 gene of
Saccharomyces cerevisiae from a recombinant Escherichia coli. Applied
Microbiology and Biotechnology, 53, 596-600.
91
Gaid, M. M., Sircar, D., Muller, A., Beurle, T., Liu, B., Ernst, L., Hansch, R., and
Beerhues,L. (2012). Cinnamate: CoA ligase initiates biosynthesis of a
benzoate derived xanthone phytoalex in hypericum calycinum cell cultures.
Plant Physiology Preview, doi:10.1104/pp.112.204180.
Garcia, C. V., Stevenson, R. J., Atkinson, R. G., Winz, R. A., and Quek, S. (2013).
Changes in the bound aroma profiles of ‘Hayward’ and ‘Hort16’ kiwifruit
(Actinidia spp.) during ripening and GC-olfactometry analysis. Food
Chemistry, 137, 45-54.
Geiger, O., Lopez-Lara, I. M., and Sohlenkamp, C. (2012).
Phosphatidylcholine biosynthesis and function in bacteria. Biochemica et
Biophysica Acta, xxx-xxx
Gika, H. G., Theodoridis, G. A., Vrhovsek, U., and Mattivi, F. (2012). Quantitative
profiling of polar primary metabolites using hydrophilic interaction ultrahigh
performance liquid chromatography-tandem mass spectroscopy. Journal of
Chromatography A, 1259, 121-127.
Gillooly, D. J., Morrow, I. C., Lindsay, M., Gould, R., Bryant, N. J., Gaullier, J.,
Parton, R.G., and Stenmark, H. (2000). Localization of phosphatidylinositol
3-phosphate in yeast and mammalian cells. The EMBO Journal, 19(17),
4577-4588.
Goffeau, A., Barrell, B. G., Bussey, H., Davis, R. W., Dujon, B., Feldmann, H.,
Galibert, F., Hoheisel, J. D., Jacq, C., Johnston, M., Louis, E. J., Mewes,
H. W., Murakami, Y., Philippsen, P., Tettelin, H., and Oliver, S. G. (1996).
Life with 6000 genes. Science, 274(5287), 546-567.
Gounaris, Y. (2010). Biotechnology for the production of essential oils, flavours and
volatile ,isolates. A review. Flavor and Fragrance Journal, 25(5), 367-386.
Grebe, S. K. G., and Singh, R. J. (2011). LC-MS/MS in clinical laboratory – where
to from here? Clinical Biochemistry Review, 32, 5-31.
Hahn, J. D., and Ascerno, M. E. (2005). Cockroaches. University of Minnesota
92
Extension. Retrieved December 29, 2012 from
http://www1.extension.umn.edu/garden/insects/find/cockroaches/
Hahn-Hagerdal, B., Karhumaa, K., Larsson, C. U., Gorwa-Grauslund, M., Gorgens,
J., and van Zyl, W. H. (2005). Role of cultivation media in the development
of yeast strains for large scale industrial use. Microbial Cell Factories, 4(1),
31.
He, S., Jiang, L., Wu, B., Pan, Y., and Sun, C. (2009). Pallidol, a resveratrol dimer
from the wine, is a selective singlet oxygen quencher. Biochemical and
Biophysical Research Communications, 379, 283-287.
Healy, T.P., Copland, M.J.W., Cork, A., Przyborowska, A. and Halket, J.M. (2002).
Landing responses of Anopheles gambiae elicited by oxocarboxylic acid.
Medical and Veterinary Entomology. 16, 126-132.
Heimovitz-Friedman, A., Kan, C. C., Ehleiter, D., Persaud, R. S., McLoughlin, Z.,
Fuks, Z., and Kolenick, R. N. (1994). Ionizing radiation acts on cellular
membranes to generate ceramide and initiate apoptosis. Journal of
Experimental Medicine, 180, 525-535.
Hellstrom, A. M., Almgren, A., Carlsson, N., Svanberg, U., and Andlid, T. A.
(2012). Degradation of phytate by Pichia kudriavzevii TY13 and
Hanseniaspora guilliermondii TY14 in Tanzania togwa. International
Journal of Food Microbiology, 153(1-2), 73-77.
Heringdorf, D. M., and Jacobs, K. H. (2007). Lysophospholipid receptors:
Signalling, pharmacology and regulation by lysophospholipid metabolism.
Biochemica et Biophysica Acta, 1768, 923-940.
Heuskin, S., Verheggen, F. J., Haubruge, E., Wathelet, J., and Lognay, G. (2011).
The use of semiochemicals slow-release devices in integrated pest
management strategies. Biotechnology Agronomy Society and Environment,
15(3), 459-470.
Hsieh, H., Wang, S., Chen, T., Huang, Y., and Chen, M. (2012). Effect of cow’s and
93
goat’s milk as fermentation media on the microbial ecology of sugary kefir
grains. International Journal of Food Microbiology, 157, 73-81.
Humphris, S. N., Bruce, A., Buultjens, E., and Wheatley, R. E. (2002). The effects of
volatile microbial secondary metabolites on protein synthesis in Serpula
lacrymans. FEMS Microbiology Letters, 210, 215-219.
Iacumin, L., Manzano, M., Cecchini, F., Orlic, S., Zironi, R., and Comi, G. (2012).
Influence of specific fermentation conditions on natural microflora of
pomace in “grappa” production. World Journal of Microbiology and
Biotechnology, 28, 1747-1759.
Iida, Y., Tominaya, Y., and Sugawara, R. (1981). Attractiveness of
methylcyclohexyl n-alkanoates to the German cockroach. Agricultural
Biology and Chemistry, 45(2), 469-473.
Isono, N., Hayakawa, H., Usami, A., Mishima, T., and Hisamatsu, M. (2012). A
Comparative study of ethanol production by Issatchenkia orientalis strains
under stress conditions. Journal of Bioscience and Bioengineering, 113(1),
76-78.
Iwashina, T. (2003). Flavonoid function and activity to plants and other organisms.
Biological Sciences in Space, 17(1), 24-44.
Izhaki, I. (2002). Emodin – a secondary metabolite with multiple ecological
functions in higher plants. New Phytologist, 155, 205-217.
Jemal, M. (2000). High throughput quantitative bioanalysis by LC/MS/MS.
Biomedical Chromatography, 14, 422-429.
Jiang, H., Zhou, L., Zhang, J. M., Doug, H. F., Hu, Y. Y., and Jiang, M. S. (2008).
Potential of Periplaneta fuliginosa densovirus as a biocontrol agent for
smoky-brown cockroach, P. fuliginosa. Biological Control, 46, 94-100.
Jiang, J. (1995). Volatile metabolites produced by Kluyveromyces lactis and their
changes during fermentation. Process Biochemistry, 30(7), 635-640.
94
Johnson, D. W. (2005). Contemporary clinical usage of LC/MS: Analysis of
biologically important carboxylic acids. Clinical Biochemistry, 38, 351-361.
Jung, P. P., Friedrich, A., Souciet, J., Louis, V., Potier, S., de Montigny, J., and
Schacherer, J. (2010). Complete mitochondrial genome sequences of the
yeast Pichia farinose and comparative analysis of closely related species.
Current Genetics, 56, 507-515.
Kalyani, A., Prapulla, S. G., and Karanth, N. G. (2000). Study on the production of
6-pentyl-α-pyrone using two methods of fermentation. Applied Microbial
Biotechnology, 53, 610-612.
Kanakis, C. D., Daferera, D. J., Tarantilis, P. A., and Polissiou, M. G. (2004).
Qualitative determination of volatile compounds and quantitative evaluation
of safranal and 4-Hydroxy-2,6,6-trimethyl-1-cyclohexane-1-carboxaldehyde
(HTCC) in Greet saffron. Journal of Agricultural and Food Chemistry, 52,
4515-4521.
Kasthuri, T., Gowdhaman, D., and Ponnusami, V. (2012). Production of ethanol
from water hyacinth (Eichhornia crassipes) by Zymomonas mobilis CP4:
Optimization studies. Asian Journal of Scientific Research, 5(4), 285-289.
Karimifar, N., Gries, R., Khaskin, G., and Gries, G. (2011). General food
Semiochemicals attract omnivorous German cockroaches, Blattella
germanica. Agricultural and Food Chemistry, 59, 1330-1337.
Katsuki, H., and Bloch, K. (1967). Studies on the biosynthesis of ergosterol in yeast.
The Journal of Biological Chemistry, 242(2), 222-227.
Kaur, U., Oberoi, H. S., Bhargav, V. K., Sharma-Shivappa, R., and Dhaliwal, S. S.
(2012). Ethanol production from alkali- and ozone-treated cotton stalks using
thermotolerant Pichia kudriavzevii HOP-1. Industrial Crops and Products,
37(1), 219-226.
KEGG: Kyoto Encyclopedia of Gene and Genomes. Retrieve December 29, 2012
from http://www.genome.jp/kegg/
95
Kells, S. A. (2005). Bait aversion by German cockroaches (Dictyoptera:
Blattellidee): The Influence and interference of nutrition. Proceedings of the
Fifth International Conference on Urban Pests. P & Y Design Network,
Malaysia: ICUP, 419-422.
Kim, J. H., and Mullin, C. A. (2007). An isorhamnetin rhamnoglycoside serves as a
costimulant for sugars and amino acids in feeding responses of adult western
corn rootworms.(Diabrotica virgifera virgifera) to corn (zea may) pollen.
Journal of Chemistry and Ecology, 33, 501-512.
Kite, G. O., and Hetterschieid, W. L. A. (1997). Inflorescence odours of
amorphophallus and Pseudodracontium (araceae). Phytochemistry, 46(1),
71-75.
Klose, C., Surma, M.A., Gerl, M.J., Meyenhofer, F., Shevchenko, A. and Simons, K.
(2012). Flexibility of a eukaryotic lipidome – insights from yeast lipidomics.
PLOS ONE. 7(4), e35063.
Koehler, P. G., Oi, F. M., and Branscome, D. (2007). Cockroaches and their
management. University of Florida IFAS extension. Retrieved September
26, 2012 from www.edis.ifas.ufl.edu/ig082
Kooijman, S. A. L. M., Segel, L. A. (2005). How growth affects the fate of cellular
Metabolites. Bulletin of Mathematical Biology, 67, 57-77.
Kotze, M. J., Jurgens, A., Johnson, S. D., and Hoffmann, J. H. (2010). Volatiles
associated with different flower stages and leaves of Acacia Cyclops and
their potential role as host attractants for Dasineura dielsi (Diptera:
Cecidomyiidae). South African Journal Botany, 76, 701-709.
Krings, U., and Berger, R. G. (1998). Biotechnological production of flavors and
fragrances. Applied Microbiology and Biotechnology, 49, 1-8.
Krokida, M. K., and Philippoulos, C. (2006). Volatility of apples during air and
freeze drying. Journal of Food Engineering, 73, 135-141.
96
Kugimiya, S., Nishida, R., Kuwahara, Y. and Sakuma, M. (2002). Phospholipid
composition and pheromonal activity of nuptial secretion of the male
German cockroach, Blattella germanica. Entomologia Experimentalis et
Applicata. 104, 337-344.
Kurtzman, C. P., Smiley, M. J., and Johnson, C. J. (1980). Emendation of the genus
Issatchenkia Kudriavzev and comparison of species by deoxyribonucleic acid
reassociation, mating reactions, and ascospore ultrastructure. International
Journal of Systematic Bacteriology, 30(2), 503-513.
Lacerda, I. C. A., Miranda, R. L., Borelli, B. M., Numes, A. C., Nardi, R. M. D.,
Lachance, M., and Rosa, C. A. (2005). Lactic acid bacteria and yeasts
associated with spontaneous fermentations during the production of sour
cassava starch in Brazil. International Journal of Food Microbiology, 105,
213-219.
Lakshmanan, D., and Radha, K. V. (2012). An effective quantitative estimation of
lovastatin from Pleurotus ostreatus using UV and HPLC. International
Journal of Pharmacy and Pharmaceutical Sciences, 4(4), 462-464.
Landrault, N., Larronde, F., Delaunay, J., Castagnino, C., Vercauteren, J., Merillon,
J., Gasc, F., Cros, G., and Teissedre, P. (2002). Levels of stilbene oligomers
and astilbin in French varietal wines and in grapes during noble rot
development. Journal of Agricultural and Food Chemistry, 50, 2046-2052.
Larsen, T. O., and Frisvad, J. C. (1995). Characterization of volatile metabolites
from 47 Penicillium taxa. Mycology Research, 99(10), 1153-1166.
Larue, F., Lafon-Lafourcade, S., and Ribereau-Gayon, P. (1980). Relationship
between the sterol content of yeast cells and their fermentation activity in
grape must. Applied and Environmental Microbiology, 39(4), 808-811.
LeBouf, R. F., Schuckers, S. A., and Rossner, A. (2010). Preliminary assessment of
a model to predict mold contamination based on microbial volatile organic
compound profiles.Science of the Total Environment, 408, 3648-3653.
97
Lee, P. R., Ong, Y. L., Yu, B., Curran, P., and Liu, S. Q. (2010). Profile of volatile
compounds during papaya juice fermentation by a mixed culture of
Saccharomyces cerevisiae and Williopsis saturnus. Food Microbiology, 27,
853-861.
Lodolo, E. J., Kock, J. L. F., Axcell, B. C., and Brooks, M. (2008). The yeast
Saccharomyces cerevisiae – the main character in beer brewing. FEMS Yeast
Research, 8, 1018-1036.
Longo, M. A., and Sanroman, M. A. (2006). Production of food aroma compounds.
Food Technology Biotechnology, 44(3), 335-353.
Lorenzo, M. G., Manrique, G., Pires, H. H. R., de Brito Sanchez, M. G., Diotaiuti,
L., and Lazzari, G. R. (1999). Yeast culture volatiles as attractants for
Rhodius prolixus: Electroantennogram responses and captures in yeast-baited
traps. Acta Tropica, 72,119-124.
Loscos, N., Hernandez-Orte, P., Cacho, J., and Ferreira, V. (2007). Release and
formation of varietal aroma compounds during alcohol fermentation from
nonfloral grape odorless flavor precursors fractions. Journal of Agricultural
and Food Chemistry, 55, 6674-6684.
Lucas, J. R., and Invest, J. F. (1993). Factors involved in the successful use of
hydramethylnon baits in household and industrial pest control. Proceedings
of the first International Conference in Urban Pests, 99-106.
Luo, W., D’Angelo, E. M., and Coyne, M. S. (2007). Plant secondary metabolites,
biphenyl and hydroxypropyl-β-cyclodextrin effects on aerobic
polychlorinated biphenyl removal and microbial community structure in
soils. Soil Biology and Biochemistry, 39, 735-743.
Luo, B., Groenk, K., Takors, R., Wandrey, C., and Oldiges, M. (2007). Simultaneous
determination of multiple intracellular metabolites in glycolysis, pentose
phosphate pathway and tricarboxylic acid cycle by liquid chromatography –
mass spectrometry. Journal of Chromatography A, 1147, 153-164.
98
Maffei, M. E. (2010). Sites of synthesis, biochemistry and functional role of plant
volatiles. South African Journal of Botany, 76, 612-631.
Magan, N., Pavlon, A., and Chrysanthakis, I. (2001). Milk-sence: a volatile sensing
system recognizes spoilage bacteria and yeasts in milk. Sensors and
Actuators, B 72, 28-34.
Magan, N., and Evans, P. (2000). Volatiles as an indicator of fungal activity and
differentiation between species, and the potential use of electronic nose
technology for early detection of grain spoilage. Journal of Stored Products
Research, 36, 319-340.
Maghsoodi, V., Razavi, J., and Yaghmaei, S. (2009). Production of chitosan by
submerged fermentation from Aspergillus niger. Transactions C: Chemistry
and Chemical Engineering, 16(2), 145-148.
Monobe, M., Arimoto-Kobayashi, S., and Ando, K. (2003). Β-Pseudouridine, a beer
component, reduces radiation- induced chromosome aberrations in human
lymphocytes. Mutation Research, 538, 93-99.
Mas, S., Villa-Boas, S. G., Hansen, M. E., Akesson, M., and Nielsen, J. (2006). A
comparison of direct infusion MS and GC-MS for metabolic footprinting of
yeast mutants. Biotechnology and Bioengineering, 96(5), 1014-1022.
Matuszewski, B. K., Constanzer, M. L., and Chavez-Eng, C. M. (1998). Matrix
effect in quantitative LC/MS/MS analysis of biological fluids: A method for
determination of finasteride in human plasma at pictogram per milliliter
concentrations. Analytical Chemistry, 70, 882-889.
Meinwald, J. (1990). Alkaloids and isoprenoids as defensive and signaling agents
among insects. Pure & Applied Chemistry, 62(7), 1325-1328.
Morao, A., Brites Alves, A. M., and Cardoso, J. P. (2001). Ultrafiltration of
demethylchlrotetracycline industrial fermentation broths. Separation and
Purification Technology, 22-33, 459-466.
99
Mukherjee, S., Zha, X., Tabas, I., and Maxfield, F. R. (1998). Cholesterol
distribution in living cells: Fluorescence imaging using dehydroergosterol as
a fluorescent cholesterol analog. Biophysical Journal, 75, 1915-1925.
Nalyanya, G., and Schal, C. (2001). Evaluation of attractants for monitoring
populations of the German cockroach (Dictyoptera Blattellidae). Journal of
Economic Entomology, 94(1), 208-214.
Nevoigt, E. (2008). Progress in metabolic engineering of Saccharomyces cerevisiae.
Microbiology and Molecular Biology Review, 72(3), 379-412.
N’guessan, K. F., Brou, K., Jacques, N., Casaregola, S., and Dje, K. M. (2011).
Identification of yeasts during alcoholic fermentation of tchapalo, a
traditional sorghum beer from Cote d’Ivoire. Antonie van Leeuwenhoek , 99,
855-864.
Nguyen, M. T., and Ranamukhaarachchi, S. L. (2010). Soil-borne antagonists for
biological control of bacterial wilt disease caused by Ralstonia solanacearum
in tomato and pepper. Journal of Pathology, 92(2), 395-406.
Nguyen, M. T., Ranamukhaarachchi, S. L., and Hannaway, D. B. (2011). Efficacy of
antagonistic strains of Bacillus megaterium, Enterobacter cloacae, Pichia
guilliermondii and Candida ethanolica against bacterial wilt disease of
tomato. Journal of Phytology, 3(2), 01-10.
Nishida, R., Enomoto, H., Shelly, T. E., and Ishida, T. (2009). Sequestration of 3-
oxygenated α- ionone derivatives in the male rectal gland of the solanaceous
fruit fly, Bactrocera latifrons. Entomologi Experimentalis et Applicata, 131,
85-92.
Norin, T. (2007). Semiochemicals for insect pest management. Pure and Applied
Chemistry, 79, 2129-2136.
Nout, M. J. R., and Bartelt, R. J. (1998). Attraction of a flying mitidulid
(Carpohpilus humeralis) to volatiles produced by yeasts grown on sweet corn
and a corn-based medium. Journal of Chemical Ecology, 24(7), 1217-1239.
100
Nova, M. X. V., Schuler, A. R. P., Brasileiro, B. T. R. V., and Morais Jr., M. A.
(2009).Yeast species involved in artisanal cachaca fermentation in three
stills with different technological levels in Pernambuco, Brasil. Food
Microbiology, 26, 460-466.
Nuobariene, L., Hansen, A. S., and Arneborg, N. (2012). Isolation and identification
of phytase-active yeasts from sourdoughs. LWT-Food Science and
Technology, 48,190-196.
Oberoi, H. S., Babbar, N., Sandhu, S. K., Dhahival, S. S., Kaur, U., Chadha, B. S.,
and Bhargav, V. K. (2012). Ethanol production from alkali- treated rice straw
via simultaneous saccharification and fermentation using newly isolated
thermotolerant Pichiakudriavzevii HOP-1. Journal of Industrial
Microbiology and Biotechnology, 39, 557-566.
Ohvo-Rekila, H., Ramstedt, B., Leppimaki, P., and Slotte, J. P. (2002). Cholesterol
interactions with phospholipids in membranes. Progress in Lipid Research,
41,66-97.
Olczak, M., and Guillen, E. (2006). Characterization of a mutation and an alternative
Splicing of UDP-galactose transporter in MDCK-RCA cell line. Biochemica
et Biophysica Acta, 1763, 82-92.
Oppliger, F.Y., Guerin, P.M. and Vlimant, M. (2000). Neurophysiological and
Behavioural evidence for an olfactory function for the dorsal organ and a
gustatory one for the terminal organ in Drosophila melanogaster larvae.
Journal of Insect Physiology. 46, 135-144.
Pai, H. (2013). Multidrug resistant bacteria isolated from cockroaches in long-term
care facilities and nursing homes. Acta Tropica, 125(1), 18-22.
Pajot, H. F., Delgado, O. D., de Figueroa, L. I. C., and Farina, J. I. (2011).
Unraveling the decolourizing ability of yeast isolates from dye-polluted and
virgin environments: an ecological and taxonomical review. Antonie van
Leeuwenhoek, 99, 443-456.
101
Papalexandratou, Z., and De Vuyst, L. (2011). Assessment of the yeast species
composition of cocoa bean fermentations in different cocoa-producing
regions using denaturing gradient gel electrophoresis. FEMS Yeast Research,
11, 564-574.
Park, K. S., Lee, H. Y., Lee, S. Y., Kim, M., Kim, S. D., Kim, J. M., Yun, J., Im, D.,
and Bae, Y. (2007). Lysophosphatidylethanolamine stimulates chemotactic
migration and cellular invasion in SK-OV3 human ovarian cancer cells:
Involvement of pertussis toxin-sensitive G-protein coupled receptor. FEB
Letters, 581, 4411-4416.
Park, J. P., Kim, S. W., Hwang, H. J., and Yun, J. W. (2000). Optimization of
submerged culture conditions for the mycelia growth and exo-biopolymer
production by Cordyceps militaris. Letters in Applied Microbiology, 33, 76-
81.
Perrut, M. (2000). Supercritical fluid applications: Industrial development and
economic issues. Industrial and Engineering Chemistry Research, 39, 4531-
4535.
Pickersky, E., and Gershenzo, J. (2002). The formation and function of plant
volatile: Perfurme for pollinator attraction and defense. Current Opinion in
Plant Biology, 5, 237-243.
Piskur, J., Rozpedowska, E., Polakova, S., Merico, A., and Compagno, C. (2006).
How did Saccharomyces evolve to become a good brewer? Trends in
Genetics, 22(4), 183-186.
Pinson, B., Vaur, S., and Seagot, I (2009). Metabolic intermediates selectively
stimulate transcription factor interaction and modulate phosphate and purine
pathways.Genes and Development, 23, 1399-1407.
Pontes, M., Pereira, J., and Camara, J. S. (2012). Dynamic headspace solid-phase
microextraction combined with one-dimensional gas chromatography-mass
spectrometry as a powerful tool to differentiate banana cultivars based on
their volatile metabolic profile. Food Chemistry, 134, 2509-2520.
102
Quesada-Moraga, E., Santos-Quiros, R., Valverde-Garcia, P., and Santiago-Alvarez,
C. (2004). Virulence, horizontal transmission, and sublethal reproductive
effects of Metarhizium anisopliae (Anamorphic fungi) on the German
cockroach (Blattodea: Blattellidae). Journal of Invertebrate Pathology, 87,
51-58.
Ragaert, P., Devlieghere, F., Loos, S., Dewulf, J., Van Langenhove, H., and
Debevere, J. (2006). Metabolite production of yeasts on a strawberry-agar
during storage at 7oC in air and low oxygen atmosphere. Food
Microbiology, 23, 154-161.
Reddy, L. V. A., and Reddy, O. V. S. (2011). Effect of fermentation conditions on
yeast growth and volatile composition of wine produced from mango
(Mangifera indica L.) fruit juice. Food and Bioproducts Processing, 89, 487-
491.
Regnier, F. E., and Law, J. H. (1968). Insect pheromones. Journal of Lipid Research,
9, 541-551.
Rivault, C. (1989). Spatial distribution of the cockroach, Blattella germanica, in a
swimming-bath facility. Entomologia Experimentalis et Applicata, 53,247-
255.
Rodriguez-Naranjo, M. I., Gil-Izquierdo, A., Troncoso, A. M., Cantos-Villar, E., and
Garcia-Parrilla, M. C. (2011). Melatonin is synthesised by yeast during
alcoholic fermentation in wines. Food Chemistry, 126, 1608-1613.
Romano, P., Fiore, C., Paraggio, M., Caruso, M., and Capece, A. (2003). Function of
yeast species and strains in wine flavor. International Journal of Food
Microbiology, 86(1- 2), 169-180.
Rossouw, D., Du Toit, M., and Bauer, F. F. (2012). The impact of co- inoculation
with Oenococcus oeni on the transcriptome of Saccharomyces cerevisiae and
on the flavor-active metabolite profiles during fermentation in synthetic
must. Food Microbiology, 29(1), 121-131.
103
Rowan, D. D. (2011). Volatile metabolites. Metabolites, 1(1), 41-63.
Rust, M. K., and Reierson, D. A. (2007). Cockroaches. University of California
Agricultural and Natural Resources. Retrieved December 28, 2012 from
http://www.ipm.ucdavis.edu/PMG/PESTNOTES/pn7467.html
Sadoudi, M., Tourdot-Marechal, R., Rousseaux, S., Steyer, D., Gallardo-Chacon, J.,
Ballester, J., Vichi, S., Guerin-Schneider, R., Caixach, J., and Alexandre, H.
(2012).Yeast-yeast interactions revealed by aromatic profile analysis of
Sauvignon Blanc wine fermented by single or co-culture of non-
Saccharomyces cerevisiae and Saccharomyces yeasts. Food Microbiology,
32, 243-253.
Saghatelian, A., Trauger, S. A., Want, E. J., Hawkins, E. G., Siuzdak, G., and
Cravatt, B. F. (2004). Assignment of endogenous substrates to enzymes by
global metabolic profiling. Biochemistry, 43, 14332-14339.
Sampranpiboon, P., Jiraratananon, R., Uttapap, D., Feng, X., and Huang, R. Y. M.
(2000).Separation of aroma compounds from aqueous solutions by
pervaporation using polyctylmetyl siloxane (POMS) and polydimethyl
siloxane (PDMS) membranes. Journal of Membrane Science, 174, 55-65.
Sasaki, K., and Takahashi, T. (2002). A flavonoid from Brassica rapa flower as the
uv-absorbing nectar guide. Photochemistry, 61, 339-343.
Scalcinati, G., Otero, J. M., van Vleet, J. R. H., Jeffries, T. W., Olsson, L., and
Nielsen, J. (2012). Evolutionary engineering of Saccharomyces cerevisiae
for efficient aerobic xylose consumption. FEMS Yeast Research, 12, 582-
597.
Scherkenbeck, J., Nentwig, G., Justus, K., Lenz, J., Gondol, D., Wendler, G.,
Dambach, M.,Nischk, F. and Graef, C. (1999). Aggregation agents in
German cockroach (Blattella germanica): Examination of efficacy. Journal
of Chemical Ecology. 25(5), 1105-1119.
Schugerl, K. (2000). Integrated processing of biotechnology products. Biotechnology
104
Advances, 18, 581-599.
Schwalbe, C. P., and Mastro, V. C. (1988). Multispecific trapping techniques for
exotic-pest detection. Agriculture, Ecosystems and Environment, 21, 43-51.
Serandour, J., Reynaud, S., Willison, J., Patouraux, J., Gaude, T., Ravanel, P.,
Lemperiere, G., and Raveton, M. (2008). Ubiquitous water soluble
molecules in aquatic plant exudates determine specific insect attraction.
PLoS One, 3(10), e3350, doi: 10.371.
Seto, Y. (1994). Determination of volatile substances in biological samples by
headspace gas chromatography. Journal of Chromatography A, 674, 25-62.
Shori, A. B. (2012). Comparative study of chemical composition , isolation and
identification of micro-flora in traditional fermented camel milk products:
Gariss, suusac, and shubat. Journal of the Saudi Society of Agricultural
Sciences, 11, 79-88.
Singh, R., Vadlani, P. V., Harrison, M. L., Bennett, G. N., and San, K. Y. (2008).
Aerobic production of isoamyl acetate by overexpression of the yeast alcohol
acetyl-transferases AFT1 and AFT2 in Escherichia coli and using low cost
fermentation ingredients. Bioprocess Biosystem Engineering Journal, 31,
299-306.
Styger, G., Prior, B., and Baur, F. F. (2011). Wine flavor and aroma. Journal of
Industrial Microbiology and Biotechnology, 38, 1145-1159.
Suh, S., Blackwell, M., Kurtzman, C. P., and Lachance, M. (2006). Phylogenetics of
Saccharomycetales, the ascomycete yeasts. Mycologia, 98(6), 1006-1017.
Sunesson, A., Vaes, W. H. J., Nilsson, C., Blomquist, G., Anderson, B., and Carlson,
R. (1995). Identification of volatile metabolites from five fungal species
cultivated in two media. Applied and Environmental Microbiology, 61(8),
2911-2918.
Swiegers, J. H., Bartowsky, E. J., Henschke, P. A., and Pretorius, I. S. (2005).
105
Microbial modulation of wine aroma and flavour. Australian Journal of
Grape and Wine Research, 11, 139-173.
Tao, B. Y. (2007). Industrial Applications for Plant Oils and Lipids.
Bioprocessing for Value-Added Products from Renewable Resources. Shang-
Tian Yang (Editor). 611-627.
Teo, H. T. R., and Saha, B. (2004). Heterogeneous catalyzed esterification of acetic
acid with isoamyl alcohol: kinetic studies. Journal of Catalysis, 228, 174-
182.
Ting, A. S. Y., Mah, S. W., and Tee, C. S. (2010). Identification of volatile
metabolites from fungal endophytes with biocontrol potential towards
Fusarium oxysporum F. sp. cubense Race 4. American Journal of
Agricultural and Biological Sciences, 5(2), 177-182.
Tirranen, L. S., and Gitelson, I. I. (2006). The role of volatile metabolites in
microbial Communities of the LSS higher plant link. Advances in Space
Research, 38, 1227-1232.
Tsiftsoglou, A. S., Tsamadou, A. I., and Papadopoulou, L. C. (2006). Heme as key
regulatorof major mammalian cellular functions: Molecular, cellular, and
pharmacological aspects. Pharmacology and Therapeutics, 111, 327-345.
Trhlin, M. and Rajchard, J. (2011). Chemical communication in the honeybee (Apis
mellifera L.): a review. Veterinarni Medicina. 56(6), 265-273.
Tsakiris, A., Koutinas, A. A., Psarianos, C., Kourkoutas, Y., and Bekatorou, A.
(2010). A New process for wine production by penetration of yeast in
uncrushed frozen grapes.Applied Biochemstry and Biotechnology, 162, 1109-
1121.
Tsuji, H. and Ono, S. (1970). Glycerol and related compounds as feeding stimulants
for cockroaches. Japanese Journal of Sanitary Zoology. 21(3), 149-156.
University of Leeds, Brewing and Microbiology, Virtual Labs @ Leeds. Retrieved
January 17, 2013 from www.virtual- labs.leeds.ac.uk/brewing/fermentation
106
Vaishnav, P., and Demain, A. L. (2010). Unexpected applications of secondary
metabolites. Biotechnology Advances, 29, 223-229.
Valdez, A. V., Garcia, L. S., Kirchmayr, M., Rodriguez, P. R., Esquinca, A. G.,
Coria, A. and Mathis, A. G. (2011). Yeast communities associated with
Artisanal mescal fermentations from Agave salmiana. Antonie van
Leeuwenhoek, 100, 497-506.
Vandamme, E. J. (2003). Bioflavours and fragrances via fungi and their enzymes.
Fungal Diversity, 13, 153-166.
Verpoorte, R., Contin, A., and Memelink, J. (2002). Biotechnology for the
production of plant secondary metabolites. Phytochemistry Reviews, 1, 13-
25.
Vukovic, G., Shtereva, D., Bursic, V., Mladenova, R., and Lazic, S. (2012).
Application of GC-MSD and LC-MS/MS for the determination of priority
pesticides in baby foods in Serbia market. LWT-Food Science and
Technology, 49, 312-319.
Walse, S. S., Lu, F., and Teal, P. E. A. (2008). Glucosylated suspensionsides, water-
soluble pheromone conjugates from the oral secretions of male anastrepha
suspense. Journal of Natural Products, 71, 1726-1731.
Wang, X. (2004). Lipid signaling. Current Opinion in Plant Biology, 7, 329-336.
Wang, C., and Bennett, G. W. (2006). Comparison of cockroach traps and attractants
for monitoring German cockroaches (Dictyoptera Blattellidae).
Environmental Entomology, 35(3), 765-770.
Wu, H., Lee, H., Horng, S., and Berec, L. (2007). Modeling population dynamics of
two cockroach species: Effect of the circadian clock, interspecific
competition and pest control. Journal of Theoretical Biology, 249, 473-486.
Wu, S., Jin, Z., Kim, J. M., Tong, Q., and Chen, H. (2009). Downstream processing
of pullulan from fermented broth. Carbohydrate Polymers, 77, 750-753.
107
Xia, H. J., and Yang, G. (2005). Inositol 1,4,5-triphosphate 3-kinases: Functions and
regulations. Cells Research, 15(2), 83-91.
Xiu, Z., and Zeng, A. (2008). Present state and perspective of downstream
processing of Biologically produced 1,3-propanediol and 2,3-butanediol.
Applied Microbial Biotechnology, 78, 917-926.
Yoon, S., Mukerjea, R., and Robyt, J. F. (2003). Specificity of yeast (Saccharomyces
cerevisiae) in removing carbohydrates by fermentation. Carbohydrate
Research, 338,1127-1132.
Zabriskie, T. M., and Jackson, M. D. (2000). Lysine biosynthesis and metabolism of
fungi.Natural Product Reports, 17, 85-97.
Zhang, L., Hach, A. and Wang, C. (1998). Molecular mechanism governing heme
signallingin yeast: a higher-order complex mediates heme regulation of the
transcriptional activator HAP1. Molecular and Cellular Biology. 18(7), 3819-
3828.
Zheng, X., Yan, Z., Han, B., Zwietering, M. H., Samson, R. A., Boekhout, T., and
Nout, M. J. R. (2012). Complex microbiota of a Chinese “Fen” liquor
fermentation starter (Fen-Daqu), revealed by culture-dependent and culture-
independent methods. Food Microbiology, 31, 293-300.
Zhou, B., Yuan, J., Zhou, Y., Yang, J., James, A. W., Nair, U., Shu, X., Liu, W.,
Kanangat, S., and Yoo, T. J. (2012). The attenuation of cockroach allergy by
DNA vaccine encoding cockroach allergen Bla g 2. Cellular Immunology,
278, 120-124.