USEFUL INFORMATION ON DISINFECTANTS AFTER CONTAMINATION WITH NOVEL SWINE ENTERIC CORONAVIRUS DISEASES (SECD)

NOVEMBRER 20, 2014

TABLE OF CONTENTS

PAGES

Recommendation on Disinfectants ..................................................................................... 1

Use of Disinfectants in winter .......................................................................................... 2

Propylene Glycol .............................................................................................................. 3

Summary Table of Useful information on Disinfectants.................................................. 4

Use Protocol when Combined with Propylene Glycol ..................................................... 6

Product Sheets ..................................................................................................................... 7

1 Stroke Environ ............................................................................................................... 8

Bioxy Enviro ...................................................................................................................... 9

Boost 1000 & Boost 1001 ............................................................................................... 11

Clorox Clean-Up Disinfectant Bleach Cleaner ................................................................ 13

Multi-Phenolique Disinfectant ....................................................................................... 15

PF 300 ............................................................................................................................. 16

Prevention Concentration .............................................................................................. 17

Synergize ........................................................................................................................ 18

Tek-Trol .......................................................................................................................... 19

Thymox ........................................................................................................................... 20

Virkon ............................................................................................................................. 22

Virocid ............................................................................................................................ 23

VirStop ............................................................................................................................ 25

Useful information on disinfectants after contamination with SECD | November 2014 1

Recommendation on Disinfectants For a disinfectant to be effective, surfaces must be thoroughly cleaned to remove all traces of organic matter.

Many virucidal disinfectants have been shown to be effective in inactivating porcine epidemic diarrhea virus (PEDV) and swine delta coronavirus (SDCV) which are novel swine enteric coronavirus diseases (SECD). One-Stroke Environ (Phenol), Virkon, Virocid, Synergize (quaternary ammonium and glutaraldehyde) and Clorox are examples of such disinfectants1. Other similar products may well be added to this list in the coming months. In this document, each proposed disinfectant has been shown to be effective against PEDV by the manufacturer. In some cases, this effectiveness has been estimated in function of that established against similar viruses (Transmittable Gastroenteritis or TGE, human coronavirus, etc.), while in other cases, it has been tested by an independent laboratory, and the report or the test results have been sent to us.

When used outdoors (e.g.: in a garden sprayer), disinfectant solutions must be regularly changed to remain effective. Ask your supplier what the manufacturer’s recommendations are in this regard. For example, a Virkon solution must be changed as soon as the pink colour indicator disappears and the solution becomes whitish, or after a maximum of 7 days, while a Virocid solution remains effective for up to 4 weeks. The solution must always be protected from sunlight and extreme temperatures.

A particularity of PEDV is its resistance to heat. It can resist heat for more than 3 hours at 50°C, but not at 60°C. Exposure for 10 minutes at 80°C is enough to inactivate the virus through heat. In comparison, Transmittable Gastroenteritis (TGE, a very similar virus) is inactive after exposure for 1 hour at 37°C.

This document is divided into three major sections. The first section, entitled “Summary Table of Useful Information on Disinfectants”, presents the contact information of distributors for each proposed disinfectant, the dilution rate recommended by the manufacturer, the contact time and, where available, information on its use below the freezing point when combined with propylene glycol. The second section, entitled “Use Protocol when Combined with Propylene Glycol” presents dilution tables for using disinfectants combined with propylene glycol. These tables are generic and may be used with any disinfectant shown to be compatible with propylene glycol, with the desired dilution rate. The third and final section, entitled “Product Sheets”, presents key information on each proposed disinfectant.

1 The following is only a proposal. The EQSP never recommends the use of any product. The EQSP recommends

that users contact their supplier to verify the recommended effectiveness, usage and contact time of products.

Useful information on disinfectants after contamination with SECD | November 2014 2

Use of Disinfectants in winter The “Use Protocol when Combined with Propylene Glycol” section presents useful dilution tables for cases when disinfectants are used in temperatures below the freezing point (0°C).

To use these tables, you must first consult the “Summary Table of Useful information on Disinfectants”, or the sheet of the product used to ensure that it is compatible when combined with propylene glycol. If the chosen disinfectant is compatible, you must choose the table corresponding to the dilution rate of the desired disinfectant and identify the percentage of the propylene glycol to obtain (based on room temperature and, as the case may be, the maximum percentage of propylene glycol recognized by the manufacturer). You will thus obtain three types of data, i.e. the volume or weight of the disinfectant, water and propylene glycol that must be used to produce 1 litre of disinfectant solution. This data can be multiplied to obtain the desired quantity of disinfectant solution.

Example:

1. Disinfectant liquid: For Synergize, at a temperature of -4°C, the manufacturer recommends a 0.8% dilution rate with a maximum of 10% of propylene glycol. The table available in the “Use Protocol when Combined with Propylene Glycol” section indicates the following three pieces of information for a 1% (1:100) dilution rate: 8 ml of Synergize, 892 ml of water and 100 ml of propylene glycol. To obtain 100 litres of solution, these amounts would thus be multiplied by 100, resulting in 800 ml of Synergize, 89.2 litres of water and 10 litres of propylene glycol.

2. Disinfectant powder: For Virkon at a temperature of -7°C, the manufacturer recommends a 1% dilution rate with a maximum of 20% of propylene glycol. The table available in the “Use Protocol when Combined with Propylene Glycol” section indicates the following three pieces of information for a 1% (1:100) dilution rate: 10 g of Virkon, 790 ml of water and 200 ml of propylene glycol. To obtain 100 litres of solution, these amounts would thus be multiplied by 100, resulting in 1 kg of Virkon, 79 litres of water and 20 litres of propylene glycol.

Useful information on disinfectants after contamination with SECD | November 2014 3

Important notes: 1. When mixing, use the following procedure, as the case may be:

a. Disinfectant liquid: always dilute the propylene glycol in water before adding the disinfectant at the required rate.

b. Disinfectant powder: always dilute the disinfectant in water before adding the propylene glycol to ensure the product is thoroughly diluted.

2. For some products, the manufacturer does not recommend using a concentration of propylene glycol above a certain level. In this case, the information is provided in the “Summary Table of Useful Information on Disinfectants” and in the product sheet. A higher concentration of propylene glycol could destabilize the solution prepared.

3. Propylene glycol increases the viscosity of solutions and some types of pumps may not be able to pump more viscous solutions as well.

4. Propylene glycol does not necessarily keep disinfectant solutions from freezing at very low temperatures (as the required concentration of propylene glycol then becomes very high) or if solutions are kept at below freezing temperatures for long periods. The following additional measures may be used to keep disinfectant solutions from freezing:

a. Store sprayers in heated buildings; b. Use sprayers that can heat the disinfectant solution; c. Use a portable heater to heat up the sprayer; d. Maintain a constant flow of the disinfectant solution in the pump.

Propylene Glycol For the above-described solutions, use pure USP or food grade propylene glycol. Propylene glycol improves fogging by fragmenting the solution drops into smaller particles, allowing for a better distribution and coverage of the sprayed surface. Propylene glycol is water-soluble and is safe for humans. It is also biodegradable. In the United States, propylene glycol is on the list of GRAS (Generally Recognized as Safe) products as an emulsifier and common food additive.

Note: Never use antifreeze for cars nor glycol ethylene, a product known to be toxic to humans and animals.

Useful information on disinfectants after contamination with SECD | November 2014 4

Summary Table of Useful information on Disinfectants Disinfectant

product Name of

distributor Address Resource-

person Telecommunications Email Active ingredient(s) Dilution rate

recommended by the manufacturer

Contact time

Protocol when

combined with

propylene glycol

1 Stroke Environ

Steris Canada Inc.

375, Britannia Road East, Unit 2 Mississauga, ON

Sylvain Simard

Tel.:1 800 444-9009, ext. 76011 Cell: 514 567-8127 Fax: (440) 350-7077

sylvain_simard@steris.com Chlorophene 8.5% 0.4% 10 min. N/A

O-phenylphenol 10%

P-tert-pentylphenol 2%

Bioxy Enviro

Atomes Bio 3485 Ashby Ville Saint-Laurent, QC

Alain Brunet Tel.: 514 830-9447

Alain.brunet@atomesbio.com Sodium Carbonate Peroxyhydrate 30-70% 1% (10g/L) 15 min. 20% of PG recommend-ded

Alkyl (68% C12, 32% C14) dimethyl ethylbenzyl ammonium chloride

2%

Alkyl dimethyl benzyl ammonium chloride (C12-18)

2%

Boost 1000 & Boost 1001

Ecolab Canada 2222 Dagenais Ouest, Suite #201 Laval, QC

Éric Chatigny Tel.: 514 781-2622 Fax: 450 963-5256

eric.chatigny@ecolab.ca BOOST 1000 10% (reconstitution of

the disinfectant by mixing 50% of

1000 with 50% of 1001)

20 min. N/A C14 60%, C16 30%, C12 5%, C18 5% C12 68%, C14 32% Hydrogen peroxide

BOOST 1001 Potassium carbonate Sodium carbonate Tetrasodium EDTA

Clorox Groupe Xtreme

3165 De Miniac Saint-Laurent, QC

Martin Lavigne

Cell: 613 859-9334 martin@lavigneconsulting.ca Sodium hypochlorite 1.84% 3% 10 min. Do not combine

Gluquat 2 West Penetone

10 900, rue Sécant Montréal, QC

Alexandre Marenger

Tel.: 514 355-4660, ext. 3025 Cell: 514 248-6061 Fax: 514 355-2319

amarenger@westpenetone.com Benzalkonium chloride 8% 0.4% 10 min. N/A Didecyldimethylammonium chloride 3% Dioctyl dimethyl ammonium chloride 3% Octyl decyl dimethyl ammonium chloride 6% Glutaral 7.5%

Multi-Phénolique

Bio Agri Mix LP 226, avenue Drouin Sainte-Marie, QC

Jean-Noël Côté

Tel.: 418 386-1745 Cell: 418 570-9373 Fax: 418 386-1746

cote@bioagrimix.com Chlorophene 8.5% 1-2% N/A O-phenylphenol 10% P-tert-pentylphenol 2%

Useful information on disinfectants after contamination with SECD | November 2014 5

Disinfectant product

Name of distributor

Address Resource-person

Telecommunications Email Active ingredient(s) Dilution rate recommended by the manufacturer

Contact time

Protocol when

combined with

propylene glycol

PF 300 Bio Agri Mix LP

11 Ellens Street Mitchell, ON

Jean-Noël Côté

Tel.: 418 386-1745 Cell: 418 570-9373 Fax: 418 386-1746

cote@bioagrimix.com

Didecyldimethylammonium chloride 6.72% 1%-2%1 See prot. (max. of 60% of PG)

Glutaral 12.5%

Prevention Concentration

Farm Guard Products

36881 Dungannon Road Dungannon, ON

Mark Beaven Tel.: 519 529-3586 Cell: 519 955-7725

beaven@farmguardproducts.com Hydrogen peroxide 7% 2.5% 5 min. N/A

Synergize S.E.C. Repro 86, rue Roy Ange Gardien, De Rouville, QC

Louis Bonneville

Tel.: 1 888 446-4647 Fax: 450 293-0157

louis@secrepro.com Benzalkonium chloride 26% 0.8%

5 à 12 min.

See prot. (max. 10% of PG) Glutaral 7%

Tek-Trol S.E.C. Repro 86, rue Roy Ange Gardien, De Rouville, QC

Louis Bonneville

Tel.: 1 888 446-4647 Fax: 450 293-0157

louis@secrepro.com

Chlorophene 10.8% 0.4% 10 min. N/A

O-phenylphenol 12.1%

P-tert-pentylphenol 4.1%

Thymox Syrvet Canada 207A, des Alouettes St-Alphonse-De-Granby, QC

Réal Sauvage Tel.:1 888 779-7838 Fax: 450 361-1505

info@syrvetcanada.ca

Thymol 23% 1% 1 min. (see sheet)

30% of PG recommend-ded

Virkon Vétoquinol Canada inc.

2000, chemin Georges Lavaltrie, QC

Marc Lalonde Tel.:1 800 363-1700 Cell: 514 714-8398 Fax: 1 888 714-8386

mlalonde@vetoquinol.ca Potassium peroxymonosulfate 21.4% 1%-5%2 10 min. See prot. (max. 20% of PG)

Virocid Merial Canada Inc.

20000, Clark Graham Baie d’Urfé, QC

Dominique Brault

Tel.: 514 457-1555 Cell: 819 817-8383 Fax: 819 394-3002

Brault.Dominique@Merial.ca Benzalkonium chloride 17% 0.25% - min. 1%3 10 min. See prot. (max. of 60% of PG)

Didecyldimethylammonium chloride 7.8% Glutaral 10.7%

VirStop Constant America

7585, rue Cordner LaSalle, QC

Denis Fortin Tel.: 514 761-3339, ext. 345 Cell: 514 609-6413

dfortin@constantamerica.com Alkyldimethylbenzylammonium chloride 3-7% 0.5%-1.0% 10 min. N/A Octyldecyldimethylammonium chloride 3-7% Dimethyldioctylammonium chloride 1-5% Didecyl dimethyl ammonium chloride 1-5% Glutaraldehyde 5-10% Ethanol 1-5%

1 Truck (1%) and unloading dock (2%). 2 Disinfection at 1%, spraying (0.25L/m2 (or 10pi2)) at 1% or fogging (1-4L/100m3 (or 3500pi3) at 1%-5%. 3 Cleaning, disinfection and sanitizing (1L/10m2) at 0.25-0.5%, disinfection of transport equipment at 0.5-1%, vehicle tire wash at 1% and foot wash at a minimum of 1%.

Useful information on disinfectants after contamination with SECD | November 2014 6

Use Protocol when Combined with Propylene Glycol Follow the dilution rate recommended by the manufacturer or your veterinarian and the maximum rate of propylene glycol, where applicable.

For a 0.25% (1:400) dilution rate For a 0.4% (1:256) dilution rate 1L of 0.25% disinfectant solution and propylene glycol 1L of 0.4% disinfectant solution and propylene glycol

2.5 ml (or g) of disinfectant

Water volume(ml) Propylene glycol volume(ml)

Freezing point

4 ml (or g) of disinfectant

Water volume(ml) Propylene glycol volume(ml)

Freezing point °C °F °C °F

972.5 25 (2.5%) 0 32 971 25 (2.5%) 0 32 947.5 50 (5%) -2 28 946 50 (5%) -2 28 897.5 100 (10%) -4 25 896 100 (10%) -4 25 797.5 200 (20%) -7 20 796 200 (20%) -7 20 697.5 300 (30%) -12 10 696 300 (30%) -12 10 597.5 400 (40%) -21 -5 596 400 (40%) -21 -5

For a 0.8% (1:128) dilution rate For a 1.0% (1:100) dilution rate 1L of 0.8% disinfectant solution and propylene glycol 1L of 1.0% disinfectant solution and propylene glycol

8 ml (or g) of disinfectant

Water volume(ml) Propylene glycol volume(ml)

Freezing point

10 ml (or g) of disinfectant

Water volume(ml) Propylene glycol volume(ml)

Freezing point °C °F °C °F

967 25 (2.5%) 0 32 965 25 (2.5%) 0 32 942 50 (5%) -2 28 940 50 (5%) -2 28 892 100 (10%) -4 25 890 100 (10%) -4 25 792 200 (20%) -7 20 790 200 (20%) -7 20 692 300 (30%) -12 10 690 300 (30%) -12 10 592 400 (40%) -21 -5 590 400 (40%) -21 -5

For a 2.0% (1:50) dilution rate For a 2.5% (1:40) dilution rate 1L of 2.0% disinfectant solution and propylene glycol 1L of 2.5% disinfectant solution and propylene glycol

20 ml (or g) of disinfectant

Water volume(ml) Propylene glycol volume(ml)

Freezing point

25 ml (or g) of disinfectant

Water volume(ml) Propylene glycol volume(ml)

Freezing point °C °F °C °F

955 25 (2.5%) 0 32 950 25 (2.5%) 0 32 930 50 (5%) -2 28 925 50 (5%) -2 28 880 100 (10%) -4 25 875 100 (10%) -4 25 780 200 (20%) -7 20 775 200 (20%) -7 20 680 300 (30%) -12 10 675 300 (30%) -12 10 580 400 (40%) -21 -5 575 400 (40%) -21 -5

For a 3.0% (1:33) dilution rate For a 5.0% (1:20) dilution rate 1L of 3.0% disinfectant solution and propylene glycol 1L of 5.0% disinfectant solution and propylene glycol

30 ml (or g) of disinfectant

Water volume(ml) Propylene glycol volume(ml)

Freezing point

50 ml (or g) of disinfectant

Water volume(ml) Propylene glycol volume(ml)

Freezing point °C °F °C °F

945 25 (2.5%) 0 32 925 25 (2.5%) 0 32 920 50 (5%) -2 28 900 50 (5%) -2 28 870 100 (10%) -4 25 850 100 (10%) -4 25 770 200 (20%) -7 20 750 200 (20%) -7 20 670 300 (30%) -12 10 650 300 (30%) -12 10 570 400 (40%) -21 -5 550 400 (40%) -21 -5

Useful information on disinfectants after contamination with SECD | November 2014 7

Product Sheets

Useful information on disinfectants after contamination with SECD | November 2014 8

Name of product: 1 Stroke Environ DIN: 00611433

Manufacturer: Steris Corporation 5960 Heisley Road Mentor OHIO 44060

Website: www.sterislifesciences.com

Distributor: Steris Canada Inc. 375, Britannia Road East, Unit 2 Mississauga ON L4Z 3E2

Contact person: Sylvain Simard Tel.: 1 800 444-9009 Ext. 76011 Cell: 514 567-8127 Fax: 440 350-7077 Email: sylvain_simard@steris.com Website: www.sterislifesciences.com Class:: Disinfectant Type of product: Liquid Known to be effective against:

PEDV ☒ Deltacoronavirus ☐ TGE ☐ Swine coronavirus ☐ Human coronavirus ☐

Additional notes: References: HOFMANN M, and R. WYLER (1989). ”Quantitation, biological and

physicochemical properties of cell culture-adapted porcine epidemic diarrhea coronavirus (PEDV)”, Vet Microbiol, Jun; 20(2), p. 131-142.

POSPISCHIL A, STUEDLI A, KIUPEL M, et al (2002). ”Update on porcine epidemic diarrhea”, J Swine Health Prod. Vol. 10(2), p. 81-85.

SIMARD, Sylvain (June19, 2014). Info STERIS, [email to Sébastien Simard], [online], Sylvain_Simard@steris.com

Active ingredient(s) Concentration Chlorophene 8.5% O-phenylphenol 10% P-tert-pentylphenol 2% Dilution rate recommended by the manufacturer: 0.4% Contact time recommended by the manufacturer: 10 minutes Protocol when combined with propylene glycol: N/A

Useful information on disinfectants after contamination with SECD | November 2014 9

Name of product: Bioxy Enviro DIN: 02431734 Manufacturer: Bioxy AFD inc.

3485 Ashby Ville Saint-Laurent QC H4R 2K3

Website: www.bioxyafd.com (site under construction) Contact person (tech.): Fadi Dagher, Ph.D. Tel.: 514 745-2597 Cell: Fax: 514 745-5176 Email: info@bioxyafd.com Distributor: Atomes Bio

3485 Ashby Ville Saint-Laurent QC H4R 2K3

Website: www.atomesbio.com Contact person: Alain Brunet Tel.: 514 830-9447 Cell: Fax: Email: alain.brunet@atomesbio.com Class: Disinfectant Type of product: White powder Known to be effective against:

PEDV ☒ Deltacoronavirus ☐ TGE ☐ Swine coronavirus ☐ Human coronavirus ☐

Additional notes: Reference: GOYAL, Sagar (2014), Report – Virucidal efficacy of Bioxy H Powder

against PEDV (porcine epidemic diarrhea virus), Minneapolis, University of Minnesota.

Active ingredient(s) Concentration Sodium Carbonate Peroxyhydrate 30-70% Alkyl (68% C12, 32% C14) dimethyl ethylbenzyl ammonium chloride

2%

Alkyl dimethyl benzyl ammonium chloride (C12-18)

2%

Useful information on disinfectants after contamination with SECD | November 2014 10

Name of product: Bioxy Enviro Dilution rate recommended by the manufacturer: 1% (10g/L) Contact time recommended by the manufacturer: 15 minutes Protocol when combined with propylene glycol:

20% of propylene glycol recommended

Useful information on disinfectants after contamination with SECD | November 2014 11

Name of product: Boost 1000 & Boost 1001 DIN: Manufacturer: Website: Distributor: Ecolab Canada

2222 Dagenais Ouest, Suite #201 Laval QC H7L 5Y2

Website: www.ecolab.ca Contact person: Éric Chatigny Tel.: 514 781-2622 Cell: Fax: Email: eric.chatigny@ecolab.ca Class:: Disinfectant Type of product: Liquid Known to be effective against:

PEDV ☒ Deltacoronavirus ☐ TGE ☐ Swine coronavirus ☐ Human coronavirus ☐

Additional notes: Boost 1000 and Boost 1001 are equivalent to Sterilex Ultra Kleen

Solution, available in Canada. Sterilex Ultra Kleen Solution is the liquid version of Sterilex Ultra Powder. Once combined, solutions 1 & 2 create the same chemical reaction as Sterilex Ultra Powder. Sterilex Ultra Powder and Sterilex Ultra Kleen Solution are not available in Canada.

Reference: GOYAL, Sagar (2014), Report – Virucidal efficacy of Sterilex Ultra

Powder against PEDV (porcine epidemic diarrhea virus), Minneapolis, University of Minnesota.

Useful information on disinfectants after contamination with SECD | November 2014 12

Name of product: Boost 1000 & Boost 1001 Active ingredient(s) Concentration

Boost 1000

n-Alkyl (C14 60%, C16 30%, C12 5%, C18 5%) dimethyl benzyl ammonium chloride

n-Alkyl (C12 68%, C14 32%) dimethyl ethylbenzyl ammonium chloride

Hydrogen peroxide

Boost 1001

Potassium carbonate

Sodium carbonate

Tetrasodium EDTA

Dilution rate recommended by the manufacturer: 10% Contact time recommended by the manufacturer: 20 minutes Protocol when combined with propylene glycol: N/A

Useful information on disinfectants after contamination with SECD | November 2014 13

Name of product: Clorox Clean-Up Disinfectant Bleach Cleaner DIN: 02319470 Manufacturer: The Clorox Company of Canada Ltd.

150 Biscayne Crescent Brampton ON L6W 4V3

Website: http://clorox.ca/fr/ Distributor: Groupe Xtrême

3165 De Miniac Saint-Laurent QC H4S 1S9

Website: www.groupxtreme.com Contact person: Martin Lavigne Tel.: Cell: 613 859-9334 Fax: Email: martin@lavigneconsulting.ca Class:: Disinfectant Type of product: Liquid Known to be effective against:

PEDV ☒ Deltacoronavirus ☐ TGE ☐ Swine coronavirus ☐ Human coronavirus ☐

Additional notes:

References: HOFMANN M, and R. WYLER (1989). ”Quantitation, biological and physicochemical properties of cell culture-adapted porcine epidemic diarrhea coronavirus (PEDV)”, Vet Microbiol, Jun; 20(2), p. 131-142.

POSPISCHIL A, STUEDLI A, KIUPEL M, et al (2002). ”Update on porcine epidemic diarrhea”, J Swine Health Prod. Vol. 10(2), p. 81-85.

Active ingredient(s) Concentration Sodium hypochlorite 1.84% Dilution rate recommended by the manufacturer: 3% Contact time recommended by the manufacturer: 10 minutes Protocol when combined with propylene glycol: Do not combine.

Useful information on disinfectants after contamination with SECD | November 2014 14

Name of product: Gluquat 2 DIN: 02318393 Manufacturer: West Penetone Inc.

10 900 Secan Street Anjou QC H1J 1S5

Website: www.westpenetone.com Distributor: West Penetone

10 900, rue Sécant Montréal QC H1J 1S5

Website: www.westpenetone.com Contact person: Alexandre Marenger Tel.: 514 355-4660 Ext. 3025 Cell: 514 248-6061 Fax: 514 355-2319 Email: amarenger@westpenetone.com Class:: Disinfectant Type of product: Liquid Known to be effective against:

PEDV ☒ Deltacoronavirus ☐ TGE ☐ Swine coronavirus ☐ Human coronavirus ☐

Additional notes:

Reference: MARENGEER-DAVIES, Alexandre (June3, 2014), RE: WEST PENETONE

products, [email to Sébastien Simard], [online], amarenger@westpenetoneinc.com

Active ingredient(s) Concentration Benzalkonium chloride 8.0% Didecyl dimethyl ammonium chloride 3.0% Dioctyl dimethyl ammonium chloride 3.0% Octyl decyl dimethyl ammonium chloride 6.0% Glutaral 7.5% Dilution rate recommended by the manufacturer: 0.4% Contact time recommended by the manufacturer: 10 minutes Protocol when combined with propylene glycol: N/A

Useful information on disinfectants after contamination with SECD | November 2014 15

Name of product: Multi-Phenolique Disinfectant DIN: 00872962 Manufacturer: Bio Agri Mix LP

11 Ellens Street Mitchell ON N0K 1N0

Website: www.bioagrimix.com Distributor: Bio Agri Mix LP

11 Ellens Street Mitchell ON N0K 1N0

Website: www.bioagrimix.com Contact person: Jean-Noël Côté Tel.: 418 386-1745 Cell: 418 570-9373 Fax: 418 386-1746 Email: cote@bioagrimix.com Class:: Disinfectant Type of product: Liquid Known to be effective against:

PEDV ☒ Deltacoronavirus ☐ TGE ☐ Swine coronavirus ☐ Human coronavirus ☐

Additional notes: Same active ingredients as 1 Stroke Environ.

References: HOFMANN M, and R. WYLER (1989). ”Quantitation, biological and

physicochemical properties of cell culture-adapted porcine epidemic diarrhea coronavirus (PEDV)”, Vet Microbiol, Jun; 20(2), p. 131-142.

POSPISCHIL A, STUEDLI A, KIUPEL M, et al (2002). ”Update on porcine epidemic diarrhea”, J Swine Health Prod. Vol. 10(2), p. 81-85.

Active ingredient(s) Concentration Chlorophene 8.5% O-phenylphenol 10% P-tert-pentylphenol 2% Dilution rates recommended by the manufacturer: 1-2% Contact time recommended by the manufacturer: N/A Protocol when combined with propylene glycol: N/A

Useful information on disinfectants after contamination with SECD | November 2014 16

Name of product: PF 300 DIN: 02248634 Manufacturer: Dubois Chemicals Canada Inc.

1155 North Service Road West, Unit 6 Oakville ON L6M 3E3

Website: www.duboischemicals.com Distributor: Bio Agri Mix LP

11 Ellens Street Mitchell ON N0K 1N0

Website: www.bioagrimix.com Contact person: Jean-Noël Côté Tel.: 418 386-1745 Cell: 418 570-9373 Fax: 418 386-1746 Email: cote@bioagrimix.com Class:: Disinfectant Type of product: Liquid Known to be effective against:

PEDV ☒ Deltacoronavirus ☐ TGE ☐ Swine coronavirus ☐ Human coronavirus ☐

Additional notes: No documentation confirming its effectiveness, but the active

ingredients and their concentrations are similar to GluQuat 2 and Synergize.

Reference: N/A

Active ingredient(s) Concentration Didecyl dimethyl ammonium chloride 6.72% Glutaral 12.5% Dilution rates recommended by the manufacturer: 1% (truck) and 2% (unloading dock) Contact time recommended by the manufacturer: N/A Protocol when combined with propylene glycol: Max. of 60% of propylene glycol

Useful information on disinfectants after contamination with SECD | November 2014 17

Name of product: Prevention Concentration DIN: 02361396 Manufacturer: Virox Technologies Inc.

2770 Coventry Road Oakville ON L6H 6R1

Website: http://www.virox.com/ Distributor: Farm Guard Products

36881 Dungannon Road Dungannon ON N0M 1R0

Website: www.farmguardproducts.com Contact person: Tel.: 519-529-3586 Cell: 519-955-7725 Fax: Email: beaven@farmguardproducts.com Class:: Disinfectant Type of product: Liquid Known to be effective against:

PEDV ☐ Deltacoronavirus ☐ TGE ☐ Swine coronavirus ☐ Human coronavirus ☒

Additional notes: Effectiveness estimated in function of that against human

coronavirus only, without studies to confirm this.

Reference: GIVEHCHI, Babak (2014), Re: PREVention Concentrate effective against

Porcine Epidemic Diarrhea virus (PEDv), Oakville, Virox Technologies inc. Unpublished paper.

Active ingredient(s) Concentration Hydrogen peroxide 7% / p/p Dilution rates recommended by the manufacturer: Disinfection – 2.5%

Sanitizing – 0.8% Light duty cleaning– 0.4%

Contact time recommended by the manufacturer: Disinfection – 5 minutes

Sanitizing – 30 seconds Light duty cleaning– 30 seconds

Protocol when combined with propylene glycol: N/A

Useful information on disinfectants after contamination with SECD | November 2014 18

Name of product: Synergize DIN: 02260336 Manufacturer: PRESERVE INTERNATIONAL

Po Box 17003 Reno NEVADA UNITED STATES 89511

Website: http://www.preserveinternational.com/ Distributor: S.E.C. Repro

86, rue Roy Ange Gardien, De Rouville QC J0E 1E0

Website: www.secrepro.com Contact person: Louis Bonneville Tel.: 1 888 446-4647 Cell: Fax: 450 293-0157 Email: louis@secrepro.com Class:: Disinfectant Type of product: Liquid Known to be effective against:

PEDV ☒ Deltacoronavirus ☐ TGE ☐ Swine coronavirus ☐ Human coronavirus ☐

Additional notes:

Reference: AASV (Page consulted on May 21, 2014), Evaluation of a Protocol for

Sanitizing PEDV – Positive Transport Vehicles, [online], http://www.aasv.org/news/story.php?id=7225

Active ingredient(s) Concentration Benzalkonium chloride 26.0% Glutaral 7.0% Dilution rate recommended by the manufacturer: 0.8% Contact time recommended by the manufacturer: 5-12 minutes Protocol when combined with propylene glycol: Maximum of 10% of propylene glycol

Useful information on disinfectants after contamination with SECD | November 2014 19

Name of product: Tek-Trol DIN: 02239409 Manufacturer: BIO-TEK DIVISION OF A.B.C. COMPOUNDING CO. INC.

6970 Jonesboro Road Morrow GA UNITED STATES 30260

Website: http://abccompounding.com/ Distributor: S.E.C. Repro

86, rue Roy Ange Gardien, De Rouville QC J0E 1E0

Website: www.secrepro.com Contact person: Louis Bonneville Tel.: 1 888 446-4647 Cell: Fax: 450 293-0157 Email: louis@secrepro.com Class:: Disinfectant Type of product: Liquid Known to be effective against:

PEDV ☒ Deltacoronavirus ☐ TGE ☐ Swine coronavirus ☐ Human coronavirus ☐

Additional notes:

References: HOFMANN M, and R. WYLER (1989). ”Quantitation, biological and

physicochemical properties of cell culture-adapted porcine epidemic diarrhea coronavirus (PEDV)”, Vet Microbiol, Jun; 20(2), p. 131-142.

POSPISCHIL A, STUEDLI A, KIUPEL M, et al (2002). ”Update on porcine epidemic diarrhea”, J Swine Health Prod. Vol. 10(2), p. 81-85.

Active ingredient(s) Concentration Chlorophene 10.8% O-phenylphenol 12,1% P-tert-pentylphenol 4,1% Dilution rate recommended by the manufacturer: 0.4% Contact time recommended by the manufacturer: 10 minutes Protocol when combined with propylene glycol: N/A

Useful information on disinfectants after contamination with SECD | November 2014 20

Name of product: Thymox DIN: 02333783 Manufacturer: LABORATOIRE M2 INC

4005-A Rue De La Garlock Sherbrooke QC J1L 1W9

Website: http://www.thymox.com/en/ Distributor: Syrvet Canada

207A, des Alouettes St-Alphonse-de-Granby QC J0E 2A0

Website: www.syrvetcanada.ca Contact person: Réal Sauvage Tel.: 1 888 779-7838 Cell: Fax: 450 361-1505 Email: info@syrvetcanada.ca Class:: Disinfectant Type of product: Liquid Known to be effective against:

PEDV ☐ Deltacoronavirus ☐ TGE ☐ Swine coronavirus ☐ Human coronavirus ☒

Additional notes: No specific test against Porcine Coronavirus but proven efficacy

against humane coronavirus.

References: ATS labs, FINAL STUDY REPORT, Virucidal efficacy of a Disinfectant for Use on Inanimate Environmental Surfaces Virus: Porcine Epidemic Diarrhea Virus, August 29 2014 [Document non publié]

ATS labs, FINAL STUDY REPORT, ATS labs, FINAL STUDY REPORT, Virucidal efficacy of a Disinfectant for Use on Inanimate Environmental Surfaces Virus: Human Coronavirus, March 4 2011 [Document non publié]

REORBIT (Page consultée le 11 juin 2014), THYMOX fights back against Porcine Epidemic Diarrhea, [en ligne], http://www.redorbit.com/news/health/1113088308/thymox-fights-back-against-porcine-epidemic-diarrhea/

Useful information on disinfectants after contamination with SECD | November 2014 21

Name of product: Thymox

Active ingredient(s) Concentration Thymol 23% / p/v Dilution rate recommended by the manufacturer: 1% Contact time recommended by the manufacturer: 1 minute (for transport trailers

disinfected inside in winter, follow the 10-minute contact time before going outside and being exposed to frost).

Protocol when combined with propylene glycol: 30% propylene glycol recommended

Useful information on disinfectants after contamination with SECD | November 2014 22

Name of product: Virkon DIN: 02125021 Manufacturer: VETOQUINOL N A INC

2000 Chemin Georges Lavaltrie QC J5T 3S5

Website: www.vetoquinol.ca Distributor: VETOQUINOL N A INC

2000 Chemin Georges Lavaltrie QC J5T 3S5

Website: www.vetoquinol.ca Contact person: Marc Lalonde Tel.: 1 800 363-1700 Cell: 514 714-8398 Fax: 1 888 714-8386 Email: mlalonde@vetoquinol.ca Class:: Disinfectant Type of product: Powder Known to be effective against:

PEDV ☒ Deltacoronavirus ☐ TGE ☒ Swine coronavirus ☐ Human coronavirus ☐

Additional notes: References: ATS LABS (2014). Virucidal Efficacy of a Disinfectant for Use on Inanimate

Environmental Surfaces Virus: Porcine Epidemic Diarrhea Virus, Wilmington, DuPont Experimental Station, unpublished document.

NEOGEN Corporation (2014). Porcine Epidemic Diarrhea Virus (PEDV), [online], Lexington, www.neogen.com, (Page consulted on June 20, 2014)

The Pig Site (Page consulted on June 3, 2014). DuPont Virkon S Effective for Porcine Epidemic Diarrhea Virus (PEDV), [online], http://www.thepigsite.com/swinenews/36758/dupont-virkon-s-effective-for-porcine-epidemic-diarrhoea-virus-pedv

Active ingredient(s) Concentration Potassium peroxymonosulfate 21.4% / p/p Dilution rates recommended by the manufacturer: Disinfection 1%

Spraying 1% (0.25L/m2) Fogging 1%-5% (1-4L/100m3)

Contact time recommended by the manufacturer: 10 minutes Protocol when combined with propylene glycol: Maximum of 20% of propylene glycol

Useful information on disinfectants after contamination with SECD | November 2014 23

Name of product: Virocid DIN: 02239726 Manufacturer: CID LINES

Waterpoortstraat 2 Ieper BELGIQUE 8900

Website: http://www.cidlines.com/fr-be Distributor: Merial Canada Inc.

20000, Clark Graham Baie d’Urfé QC H9X 4B6

Website: www.merial.ca Contact person: Dominique Brault Tel.: 514 457-1555 Cell: 819 817-8383 Fax: 819 394-3002 Email: Brault.dominique@merial.ca Class:: Disinfectant Type of product: Liquid Known to be effective against:

PEDV ☒ Deltacoronavirus ☐ TGE ☐ Swine coronavirus ☐ Human coronavirus ☐

Additional notes:

References: TER BEEK, Vincentter. (2014). Virocid successfully tested against PED

virus, The Pig Progress http://www.pigprogress.net/Health-Diseases/Outbreaks/2014/6/Virocid-successfully-tested-against-PED-virus-1534161W/ (Page consulted on June 19, 2014)

Cid Lines (2014). Virocid® Tested Against PEDv at 1:400 (1/3 oz/gal) at an EPA Accredited Lab, [online], Cid Lines http://www.virocid.be/virocid-tested-pedv-1400-13-oz-gal-epa-accredited-lab/ (Page consulted on June 19, 2014)

Active ingredient(s) Concentration Benzalkonium chloride 17.06% Didecyl dimethyl ammonium chloride 7.8% Glutaral 10.725%

Useful information on disinfectants after contamination with SECD | November 2014 24

Name of product: Virocid

Dilution rates recommended by the manufacturer: Cleaning, disinfection and sanitizing 0.25-0.5% (1L/10m2) Disinfection of transport equipment 0.5-1.0% Vehicle wheel wash 1.0% Foot wash, min. 1.0%

Contact time recommended by the manufacturer: 10 minutes Protocol when combined with propylene glycol: See protocol (max. 60% of PG at

51°C)

Useful information on disinfectants after contamination with SECD | November 2014 25

Name of product: VirStop DIN: Manufacturer: Website: Distributor: Constant America

7585, rue Cordner LaSalle QC H8N 2R5

Website: www.constantamerica.com Contact person: Denis Fortin Tel.: 514 761-3339 Ext. 345 Cell: 514 609-6413 Fax: Email: dfortin@constantamerica.com Class:: Disinfectant Type of product: Liquid Known to be effective against:

PEDV ☒ Deltacoronavirus ☐ TGE ☐ Swine coronavirus ☐ Human coronavirus ☐

Additional notes: The composition of VirStop is more or less the same as PF 300,

Synergize and GluQuat 2, three products that have shown some level of effectiveness against PEDV.

Reference:

Active ingredient(s) Concentration Alkyl(benzyl)dimethylammonium chloride 3-7% Octyldecyldimethylammonium chloride 3-7% Dimethyldioctylammonium chloride 1-5% Didecyl dimethyl ammonium chloride 1-5% Glutaraldehyde 5-10% Ethanol 1-5% Dilution rates recommended by the manufacturer: 0.5-1.0% Contact time recommended by the manufacturer: 10 minutes Protocol when combined with propylene glycol: N/A