Use of OPU-IVP and SO at CRV breeding program in the genomic era
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Transcript of Use of OPU-IVP and SO at CRV breeding program in the genomic era
Use of OPU-IVP and SO at CRV breeding program in the genomic era
Erik Mullaart and Sybrand Merton
Content
Introduction Genomic Selection Impact on breeding program
Selection procedures Delta Nucleus breeding program
Application of OPU-IVP and SO Insire embryo program
Holland Genetics
CRV Worldwide
Genetic products of CRV
7 million units of dairy and beef semen CRV exports semen to over 50 countries Test program:
310 Holstein bulls 60 Red Holstein bulls 35 Fleckvieh bulls 35 Jersey bulls
Genomic selection
Which calf received the best genes from the parents?
Marker selection
In early days: selection on individual genes (direct or via markers)
Now: selection based on many markers spread across the whole genome, i.e. selection on all genetic material
=GENOMIC SELECTION
(or Genomewide Selection)
Genomic Selection,What does it bring us?
Reliable breeding values at young age
higher success rate to get top bulls
lower costs for test proofs (60,000 markers for ~ € 200)
shorter generation-interval
Effects of Genomic Selection
Increased genetic improvement (30-40%)
Higher genetic level of test sires and embryo’s
Wider portfolio of commercial bulls & higher level
More outcross; less inbreeding
Availability of packs of elite young sires (InSire)
Impact on breeding program; selection procedures
Use of GS in selection procedures; method implemented by CRV
Step 1: Genotype proven bulls with reliable
EBVs (reference sires) Find markers with an effect on EBVs Calculate marker effect for each trait
Step 2: Genotype selection candidates Calculate GS Breeding Values (GBVs) with the marker
effects Select the animals with the highest GBVs
3 full sisters on Delta.Pedigree ( Oman x Jocko Besne)
Renate Raisa Rianne
Inet Tot.ext Leg. Inet Tot.ext Leg. Inet Tot.ext Leg.
EBV 165 109 106 165 109 106 165 109 106
EBV +merkers
213 109 107 178 107 103 145 107 104
BV aftertest
217 110 107 173 108 105 132 108 106
InSireBV
234 110 107 176 106 103 120 107 104
Impact on breeding program; Delta nucleus breeding program
16 years of ‘Delta nucleus’
Test heifers 4381 Selected donors 561 Before Genomic Selection
large number of test heifers per year emphasis on independent test
With Genomic Selection. limited number of test heifers optimal use of reproductive
possibilities
Delta nucleus
Selection of 800 calves GS selection
After GS-selection 110 donor heifers MOET en OPU 25 - 30 embryos per heifer
100 selected heifers tested
Selection of 10 -15 donor cows ET en OPU
=> 2500 embryos
Application of OPU-IVP and SO
Embryo production
IVP sessions: 2000 / year 1.6 embryo/ session
SO: 300 / year 4-5 embryos/ flush
Production Logistics
3 x SO at 13-17 months of age: 12 embryos Inseminate at 17 months 17 x OPU at 18-20 months: 20 embryos Calving at 26 months ----------------
32 embryos
Holland Genetics
Future Production Logistics
2 x SO at 13-15 months of age: 8 embryos 10 x OPU at 16-17 months: 12 embryos Inseminate at 18 months 17 x OPU at 19-21 months: 20 embryos Calving at 27 months ----------------
40 embryos
Holland Genetics
Working method: SO
D -12 --- Start protocol FSH 1x D -11 --- FSH 2x D -10 --- FSH 2x + Prostaglandin with 5th FSH injection D - 9 --- FSH 2x + Prostaglandin with 6th FSH injection D - 8 --- FSH 1x D - 7 --- Heat + AI (2x 8-12 hr / 16-24 hr) D - 0 --- Flush + Prostaglandin + Progesterone application D +12 --- Remove PRID D +14 --- Heat D + 23 - D + 28 --- start SO again (D - 12)
Working method: OPU-IVP
Day -1 Ovum Pick Up (OPU)In vitro maturation (IVM)
Day 0 In vitro fertilization (IVF)
Day 1 Start in vitro culture (IVC)
Day 4 Cleavage evaluation
Day 6 Prognoses embryo production
Day 7 Embryo evaluation (fresh, frozen)
Day 8 Final embryo evaluation (frozen)
Working method: OPU
OPU twice a week
(Mo, Thu) No superstimulation (para)veterinarian plus assistant Pregnant heifers and first parity donor cows
Holland Genetics
Working method: IVM
Maturation for 16 - 28 h, 38.5°C, 20% O2
Volume: transport and maturation in 0.5 ml in tubes
Holland Genetics
Working method: IVF
Semen:
Frozen-thawed
Separation semen and extender by gradient centrifugation (90% / 45%)
Volume: wells (0.5 ml),
Incubation for 24 h, 38.5 °C, 20% O2
Holland Genetics
Working method: IVC
Medium: SOFaaBSA
Volume: wells (0.5 ml)
Incubation for 6-8 days, 38.5°C, 5% O2
Holland Genetics
Working method: IVC
Embryos
Day 7 - 8, fresh / frozen
Stage 4 - 9
(Morula - Hatched Blastocyst)
Grade 1 and 2 IETS
Holland Genetics
Working method: Embryo transfer
Recipients
Healthy, fertile recipients Natural cycling Day 6, 7, 8 or 9 of estrus cycle <5% rejected recipients
150 contracted farms
Holland Genetics
Working method: Logistics OPU / IVP
OPU and SO location Transport COC’s Central IVP lab Transport of fresh
embryos Recipient herds Farmers
Holland Genetics
Results: pregnancy rates
Fresh IVP embryos: 45% - 55%
Frozen embryos: IVP 40% - 50% SO 50% - 60%
Holland Genetics
Results: Efficiency
OPU-IVP MOET
Oocytes: 8 9
Cleavage: 5,6 (70%)
Embryo: 1,6 (20%) 5 (55%)
Calf: 0,8 (10%) 3 (33%)
Insire embryo program
Number of InSire-embryos per year
InSire-embryo donors
Delta test heifers (IVP + SO) 2500 Delta donor cows (IVP + SO) 500
Total from Delta program = 3000
Marker selected Bullmothers in West-Europe. (e.g. France, Germany, Belgium, Denmark and the Netherlands
1000
Totaal 4000 InSire-embryos
Costs InSire-embryos
InSire-embryos with option € 325,-
InSire-embryos with segment option € 275,-
CRV claims /buy 15-20% of the calves born male € 1000 female € 3000
Segmentation InSire-embryos
Kg Milk
% Protein
Life span en health
Top exterior
InSire-embryos offered by Internet
1) Random embryo selection (most actual combination)
Choice of segment ( 4 ) Embryo price € 275 IVP fresh , IVP and SO frozen
2) Newest InSire embryos (most actual combination)
Choice of combination Embryo price € 325 IVP fresh , IVP and SO frozen
3) Available InSire embryos (in stock)
Embryo price € 325 IVP and SO frozen
2) Newest InSire embryos (most actual combination)
Benefits to the clients
Win-Win situation
Client InSire embryos:
- Top class calves
for a relative low price
CRV:
- increased genetics of bulls and bull dams against
an acceptable costs
Thank you for your attention!