U.S. Wheat & Barley Scab Initiative

U.S. Wheat & Barley Scab Initiative

FY07 Request for Research Project Pre-Proposals

Pre-Proposal Submission Deadline:

Tuesday, September 19, 2006, 5:00 PM EDT Submit to: USWBSI/NFO

ATTN: Susan Canty 380 Plant & Soil Sciences Building East Lansing, MI 48824-1325

For more information: www.scabusa.org [email protected] 517-355-0271 ext. 183

USWBSI FY07 Project Pre-Proposal

FY07 RFP Application Kit

TABLE OF CONTENTS

Introduction ....................................................................................................................................... ii Changes to FY07 RFP from pervious year ...................................................................................... iii Review Process ................................................................................................................................. iv Research Area Committee (RAC) Chairs/IIR Research Contact .......................................................v Instructions Cover Page .................................................................................................................................1 Project Summary Page...............................................................................................................2 Summary of Progress for Ongoing Related Projects .................................................................2 Project Description.....................................................................................................................2 References to Project Description..............................................................................................4 Research Objectives/RA Priorities Form...................................................................................4 Facilities and Equipment............................................................................................................4 Collaborative or Sub Contractual Arrangements.......................................................................4 Vita and Publication List ...........................................................................................................4 Current and Pending Support.....................................................................................................4 Budget and Budget Justification ................................................................................................4 Performance Reports..................................................................................................................5 On-line Submission Process (CBCC Pre-proposals only).........................................................6 Check List and Order of Required Items for Project Pre-Proposals..........................................7 FY07 Program Descriptions and Research Priorities (PD-RP)

Chemical, Biological & Cultural Control (CBCC)....................................................................8 Etiology, Epidemiology and Disease Forecasting (EEDF)......................................................10 Food Safety, Toxicology, & Utilization of Mycotoxin-contaminated Grain (FSTU) .............11 Genetic Engineering and Transformation (GET) ....................................................................12 Host Genetic Resources (HGR) ...............................................................................................14 Host Genetics & Genomics (HGG) .........................................................................................16 Pathogen Genetics & Genomics (PGG)...................................................................................18 Variety Development & Uniform Nurseries (VDUN).............................................................19

FORMS

(Last six pages of RFP Application)

Check List Cover Page Project Summary Page Project Objectives/RA Research Priorities Form Current and Pending Support Page Single FY07 Project Pre-Proposal Budget Page


ii FY07 RFP Application Kit

INTRODUCTION

The goal of the U.S. Wheat and Barley Scab Initiative is: “To develop as quickly as possible effective control measures that minimize the threat of Fusarium head blight (scab), including the reduction of mycotoxins, to the producers, processors, and consumers of wheat and barley”. The Initiative is guided by a Steering Committee that includes growers, farm organizations, food processors (e.g., millers, pasta manufacturers, and brewers), scientists (from Land Grant universities, USDA, and private companies), and consumer groups. Each year, the Steering Committee submits to the USDA-ARS a comprehensive and optimized research plan designed to achieve the Initiative’s goals. That plan is the Initiative’s recommendation for how the USDA-ARS can most effectively employ the funds appropriated by the U.S. Congress for collaborative scab research. The USWBSI is soliciting FY07 (approximately May 2007 – April 2008) pre-proposals for the eight research areas listed below. The USWBSI is encouraging PIs to consider the following in developing pre-proposals: 1) Integrated/Interdisciplinary Research (IIR) – Certain discipline areas and groups have established a track record of integration and collaboration in their pre-proposals (e.g. projects involving varieties, fungicides and rotations). Many PI’s, however, tend to submit pre-proposals that are narrowly focused within their area of research. The USWBSI would like to see pre-proposals that emphasize collaboration and integration, whether it involves crossing state lines or research area boundaries. Why? Because so far, the greatest progress in reducing the economic impact of scab has depended on combinations of management practices (e.g., appropriate rotations, best varieties, plus fungicides). 2) Greater focus on mycotoxins –It is recognized that while we have made progress in reducing the economic impact of head scab, and scab symptoms have been reduced, DON levels remain high under certain conditions. This continues to be a serious economic drain on the barley and wheat industries. 3) Getting the word out – The most efficacious solutions to date require stakeholder awareness and implementation: grow resistant varieties, use fungicides when the risk of scab is high, manage crop rotations effectively. Pre-proposals should contain an outreach component that increases stakeholder awareness of possible solutions to head scab. USWBSI Research Areas:

Chemical, Biological & Cultural Control (CBCC) – Electronic Submission only Etiology, Epidemiology and Disease Forecasting (EEDF) Food Safety, Toxicology, & Utilization of Mycotoxin-contaminated Grain (FSTU) Genetic Engineering and Transformation (GET) Host Genetic Resources (HGR) Host Genetics & Genomics (HGG) Pathogen Genetics & Genomics (PGG) Variety Development & Uniform Nurseries (VDUN) Integrated/Interdisciplinary Research (IRR) Projects


iii FY07 RFP Application Kit

Finalized program descriptions and research priorities for each of the eight research areas are included in this document, and can also be accessed through the USWBSI’s website at www.scabusa.org/research_rfp07.html. The USWBSI is in the process of developing a Web-based pre-proposals submission process. For FY07, a beta or test version will be utilized for pre-proposals submitted to the Chemical, Biological and Cultural Control research area. PI’s submitting a pre-proposal(s) to the CBCC research area must first complete a ‘Pre-Registration’ process. The web-based Pre-Registration form may be accessed at http://www.scabusa.org/cgi-bin/pre_req.cgi. The deadline for completing the pre-registration process (CBCC only) is August 30, 2006. See Page 5 for detailed instructions/information regarding the on-line submission of CBCC pre-proposals. Principal Investigators (PIs) should study this information prior to completing their pre-proposals, since it represents the guidelines that the reviewers will apply when considering pre-proposals. PIs are welcome and encouraged to interact with any of the Research Area Chairs (Table 1), or this office, to identify the appropriate area for their proposed work. A single PI may submit more than one project pre-proposal, however, each pre-proposal (i.e. project) will be handled as a distinct, autonomous and complete submission as per the instructions listed here. In other words, each pre-proposal must include all required materials (Application for Funding Cover Page, Project Description, CV, Publication List, Budget, etc.). Changes to FY07 RFP from previous year: This request for research project pre-proposals (RFP) contains substantial changes from the FY06 version the most significant being the acceptance of pre-proposals that focus on ‘Integrated/ Interdisciplinary Research (IIR). PIs preparing RFP’s in the IIR category should note changes on the ‘Cover Page’ and also the development of the ‘Budget’ and ‘Budget Justification’. Another important change is the inclusion of a form (follows ‘Project Summary’ page) which demonstrates a linkage between pre-proposal objectives and specific research priorities that have been identified. For example, a pre-proposal submitted to VDUN might have as one objective “to screen segregating populations for scab resistance”; this would correspond to the FY07 research objective “The breeding and release of FHB-resistant wheat and barley varieties and germplasm that are adapted to FHB-threatened states.” The form should be used for all proposals including those integrated/interdisciplinary research proposals, where the proposed research objectives should be linked to research priorities from the relevant research areas. It is important that you use the FY07 RFP Application forms, included in this booklet and posted on the Web site (http://www.scabusa.org/research_rfp07.html. The correct forms are readily identified by the inclusion of “FY07” in their titles and headers/footers. Significant changes within the application kit are marked by vertical lines on the left and right margins. Potential PIs should therefore carefully read the instructions that follow and ensure that their pre-proposals conform exactly to the described format.


iv FY07 RFP Application Kit

REVIEW PROCESS The deadline for submitting pre-proposals is Tuesday, September 19, 2006, 5:00 PM (Eastern). Pre-proposals received on or before that date will be sent for review by one of the Research Committees listed in Table 1. The Executive Committee reviews the Research Committee’s recommendations and if necessary, modifies the awards so that they fit into the FY07 congressional budget and the overall objectives of the Initiative. The final step is approval of the EC’s recommended research plan and budget by the Steering Committee in early December. Principal investigators for those pre-proposals that are recommended by the Initiative will be asked to compile and formalize their pre-proposal(s) for inclusion in the Initiative’s recommended research plan. The comprehensive plan will be sent to ARS, which will conclude its review of the Initiative’s recommendations in the early months of 2007. If you have any questions regarding this application, or the proposal process, please contact the U.S. Wheat and Barley Scab Initiative’s Networking and Facilitation Office (NFO) by phone at (517) 355-0271 ext. 183 or by e-mail at [email protected]. Please note that project pre-proposals will be regarded as confidential documents. Distribution will be limited only to parties involved with the review process. Also, depending on your institution’s requirements, the authorized organizational representative’s signature may not be required at this stage (not required for PIs affiliated with USDA-ARS).

A single copy of each project pre-proposal, plus additional copies if the pre-proposal contains colored images1, must be received in the NFO at the following address by Tuesday, September 19, 2006, 5:00 PM (Eastern):

USWBSI/NFO ATTN: Susan Canty, Manager 380 Plant and Soil Sciences Building Michigan State University East Lansing, MI 48824-1325

Pre-Registration Deadline (CBCC RA only): August 30, 2006, 5:00 PM (EDT). Pre-Proposal Submission Deadline (All RAs): September 19, 2006,

5:00 PM (EDT).

1 For pre-proposals with color images, PIs should send 15 signed, unstapled, colored, copies plus the original (signed, unstapled). All others should send only the signed original pre-proposal.


v FY07 RFP Application Kit

Table 1. Research Area Committees (RAC) Chairs/Contact for IRR pre-proposals

Research Area2 RAC Chair Email Address

Program Description and Research Priorities (PD-RP) Web Address

CBCC Marty Draper, USDA-CREES [email protected] www.scabusa.org/research_cbcc.html

EEDF Erick De Wolf, Penn State University [email protected] www.scabusa.org/research_eedf.html

FSTU Jim Pestka, Michigan State University [email protected] www.scabusa.org/research_fstu.html

GET Ron Skadsen, USDA-ARS, WI [email protected] www.scabusa.org/research_get.html

HGR Stephen Baenziger, University of Nebraska [email protected] www.scabusa.org/research_hgr.html

HGG Herb Ohm, Purdue University [email protected] www.scabusa.org/research_hgg.html

PGG Bob Bowden, USDA-ARS, KS [email protected] www.scabusa.org/research_pgg.html

VDUN Mohamed Mergoum, North Dakota State University

[email protected] www.scabusa.org/research_vdun.html

IRR Dave Van Sanford, Director of the Networking & Facilitation Office

[email protected] or [email protected] www.scabusa.org/research_rfp07.html

2 CBCC – Chemical, Biological and Cultural Control

EEDF– Etiology, Epidemiology and Disease Forecasting FSTU – Food Safety, Toxicology, and Utilization of Mycotoxin-contaminated Grain GET – Genetic Engineering and Transformation HGR – Host Genetic Resources HGG – Host Genetics and Genomics PGG – Pathogen Genetics and Genomics VDUN – Variety Development and Uniform Nurseries IRR – Integrated/Interdisciplinary Research


1 FY07 RFP Application Kit

INSTRUCTIONS FOR FORMAT AND CONTENTS OF FY07 PROJECT PRE-

PROPOSALS To facilitate pre-proposal preparation, Frequently Asked Questions (FAQs) regarding pre-proposal preparation and submission are available electronically on the USWBSI’s Website at:

http://www.scabusa.org/pdfs/fy07_rfp_faq.pdf It is important that all pre-proposals conform to the instructions provided in the FY07 Request for Pre-Proposals (RFP) Application. The USWBSI’s Networking & Facilitation Office (NFO) may return without review proposals that are not consistent with these instructions. The NFO must authorize any deviations from these instructions in advance of pre-proposal submission. Deviations may be authorized by written approval from the NFO Director ([email protected]) or Manager ([email protected]). Application for Funding Cover Page The funding application paperwork enclosed herein is a modified version of the process used by USDA’s National Research Initiative Competitive Grants Program. The pre-proposal must contain a completed Pre-proposal Application for Funding Cover Page, which will be the first page of the application. In completing this form, please note the following: • Title of Proposal (Block 6). The title of the project pre-proposal must be brief (80-character

maximum), yet represent the major thrust of the project. Because this title will be used to provide information to those who may not be familiar with the proposed project, highly technical words or phraseology should be avoided where possible. In addition, phrases such as “investigation of” or “research on” should not be used.

• Principal Investigator/Co-Investigators (Block 10). List the name of the proposing principal

investigator in this block. All other participating scientist should be listed as co-investigators. The co-investigators should be limited to those required for major scientific collaboration.

• Phone Numbers (Block 11). Please list the phone and fax number, and the e-mail address (if

available) of the principal investigator in this block. • Currently Funded Projects (Block 13). List the titles of all individual research projects that are

currently being funded in FY06 (approx. May 2006 – April 2007) in association with the USWBSI/ARS collaboration.

• Research Area (Block 16). Your Project Pre-Proposal should be constructed to address one or

more of the priorities included in the FY07 Program Description and Research Priorities. In Block 16, designate with an 'X' one Research Area whose description and priorities your project is designed to address. Research Area (RA) Program Descriptions and Research Priorities (PD-RP) are included in this application. You can also view them on the Initiative’s Web site at www.scabusa.org/research.html. If ‘Integrated/Interdisciplinary Research’ (IIR) was designated, list all research areas (acronyms) that are relevant to the IIR proposed project.

• Signatures. Sign and date the Pre-proposal Application for Funding Cover Page in the places

indicated at the bottom of the page. The principal investigator, and if applicable, all co-



investigators, must sign all project pre-proposals. Signatures for co-investigators may initially be represented by faxed copies of the cover page, but PI’s should arrange for the Networking & Facilitation Office (NFO) to receive a cover page with ink signatures of all co-investigators as soon as possible.

Project Summary Page

Each project pre-proposal must contain a completed Project Summary Page, which will be the second page of the project pre-proposal. The names and institutions of all of the principal and co-investigators should be listed on this form (if space for this information is insufficient, please enter “see attached” and provide the information on a separate sheet immediately following the Project Summary Page). The project summary itself must fit within the space indicated. The summary is not intended for the general reader; consequently, it may contain technical language relating to Fusarium head blight research. The project summary should be self-contained, include a specific description of the activity to be undertaken, and focus on the following:

• Overall project goal(s) • Project Objectives (list in numbered format); • Plans to accomplish project goal(s) within period of proposed work (one-year duration); and • Relevance of the project to the goals of the U.S. Wheat and Barley Scab Initiative with specific

reference to the research priorities listed in the FY07 Program Descriptions and Research Priorities (PD-RP).

A concise, informative project summary is essential. Project Summaries are considered part of the pre-proposal and are therefore confidential. The complete Project Summary should fit within the space allocated in the form. Summary of Progress for Ongoing Related Project(s) – Limit to a single page per project. The purpose of this section is to enable PI's to demonstrate that they have made (or are moving towards) effective use of funds for ongoing USWBSI-recommended projects related to the current pre-proposal. This includes projects which were initially funded this year (FY06). Include the following information for each relevant ongoing project:

Project Title; brief summary of activities (including non-data generating items such as hiring of personnel,

equipment acquisition, etc.), any results and/or accomplishments made to date (include actual data documenting progress made since FY06 funding was awarded; and

any information that will help reviewers understand how the current pre-proposal builds upon the ongoing project.

FY07 USWBSI Project Pre-Proposal


Project Description The Project Description should provide a clear statement of the work to be undertaken and must include: research objectives for the period of the proposed work and expected significance; relation to longer-term goals of the PI's project; and relation to the present state of knowledge in the field, to work in progress by the PI under other support and to work in progress elsewhere. The Project Description should outline the general plan of work, including the broad design of activities to be undertaken, and, where appropriate, provide a clear description of experimental methods and procedures, and plans for preservation, documentation, and sharing of data, samples, physical collections, curriculum materials and other related research and education products.

i. Format: Brevity will assist reviewers and USWBSI/NFO staff in dealing effectively with pre-proposals. Therefore, the written text of the Project Description must be five pages or less (single spaced or equivalent); ten pages or less in total including figures and tables. The Project Description should be formatted using Times New Roman (Font not smaller than 12 point) with one-inch margins. Conformance to the page limitation will be strictly enforced and may not be exceeded unless a deviation has been specifically authorized by the NFO.

ii. Content:

• Title. Same as Funding Cover Page (80-Character Maximum).

• Introduction. A clear statement of the goal(s) and supporting objectives or research questions of

the proposed project should be included. These are one-year projects; include only those research objectives which can be accomplished within one year. The most significant published work in the field under consideration, including the work of key project personnel on the current application, should be reviewed. The current status of research in this field of science should also be described including, but not limited to, any ongoing USWBSI-recommended work currently underway. Preliminary data pertinent to the proposed research may be included in this section. Present and discuss published literature that is directly related to, and supports, the proposed research (research objectives, materials and methods)

• Rationale and Significance. It is understood that head scab is a devastating disease. Acknowledgment of this fact should require no more than one sentence. Concisely present the rationale behind the proposed research. Relevance of the project to the goals of the U.S. Wheat and Barley Scab Initiative with specific reference to the priorities listed in the FY07 Program Descriptions and Research Priorities (PD-RP). If applicable, please indicate if the research proposed here could lead to successful acquisition of grant funds from other agencies.

• Research Materials and Methods. Specifically, this section must include:

The hypotheses or questions being asked; A description of the investigations and/or experiments proposed in the sequence in which

the investigation or experiments are to be preformed; Techniques and methodologies to be used in carrying out the proposed project, including the

feasibility of the techniques. (Describe in step by step fashion exactly what techniques, materials and methods will be used.);

Results expected; Means by which experimental data will be analyzed or interpreted; Means of applying results or accomplishing technology transfer, where appropriate;



Pitfalls that may be encountered; Limitations to proposed procedures; and A tentative schedule for conducting major steps involved in these investigations and/or

experiments. Please Note: Failure to address each of the issues in the bullet list will diminish the likelihood of the success of your pre-proposal being recommended for funding. References to Project Description All references cited in the Project Description should be included in bibliographic format. Although there is no established page limitation for the references, this section must include bibliographic citations only, and must not be used to provide parenthetical information outside of the project description. Project Objectives/Research Priorities Form The PI will use this form (follows ‘Project Summary’ page in RFP Application) to demonstrate a linkage between proposal objectives and specific research priorities that have been identified. The form should be used for integrated/interdisciplinary proposals too, with the research priorities from the relevant research areas linked to proposal objectives. Specific instructions are included on the form. Facilities and Equipment All facilities and major items of equipment available for use or assignment to the proposed project during the requested period of support should be described. In addition, items of nonexpendable equipment necessary to conduct and successfully conclude the proposed project should be listed (including dollar amounts), and, if funds are requested for their acquisition, justification should be included in the Budget Justification. Collaborative or Sub Contractual Arrangements If the nature of the proposed project requires collaboration or contractual arrangements with other research scientists, corporations, organizations, agencies, etc, the applicant must identify the collaborator(s) and provide a detailed explanation of the nature of the collaboration. Evidence in the form of a letter of intent from all collaborators must be provided to assure reviewers that collaborators or sub-contractors involved have agreed to render the indicated services. The letter of intent must include specific details describing the services the collaborator will provide. Vita and Publication List (Maximum four pages per Scientist) To assist peer reviewers in assessing the competence and experience of the proposed project staff, all personnel who will be involved in the proposed project must be identified clearly. The following should be included for all personnel (PI, Co-Investigators, and secondary scientists (i.e. post-docs, graduate students)) who are expected to work on the project in a significant fashion (e.g. expectation of co-authorship on ensuing publications), whether or not funds are sought for this support: • Curriculum Vita (C.V.). The C.V. should be limited to a presentation of academic and research

credentials, e.g., educational, employment and professional history, and honors and awards. Unless pertinent to the project, do not include meetings attended, seminars given, or personal data such as birth date, marital status, or community activities.



• Publication List. A chronological list of all publications in refereed journals during the past five years, including those in press, should be provided for each professional project member for whom a curriculum vita is provided. Non-refereed technical publications that have relevance to the proposed project may be included.

The C.V. and Publication List combined must not exceed four pages per scientist. C.V.’s for collaborators or sub-contractors are not required. Current and Pending Support All project pre-proposals must contain a completed Current and Pending Support Page listing other current public or private support to which key personnel identified in the pre-proposal have committed portions of their time, whether or not salary support for person(s) involved is included in the budget. Analogous information must be provided for any pending proposals that are being considered by, or that will be submitted in the near future to, other possible sponsors, including other USDA Programs or agencies, and the USWBSI. Concurrent submission of identical or similar proposals to the other possible sponsors will not prejudice pre-proposal review or evaluation by the USWBSI for this purpose. However, a project pre-proposal that duplicates or overlaps substantially with a proposal already reviewed and funded (or to be funded) by another organization or agency will not be recommended under this program. The third column (‘Requested/Award Amount’) on the ‘Current and Pending Support Page’ has been divided into two sub-columns: Total $ Amount and PI $ Allocation. For each grant/proposal listed, include both the total (‘Total $ Amount’) and the amount you have or may receive (‘PI $ Allocation’). Budget and Budget Justification

Please complete the ‘Single Project Pre-Proposal Budget Page’ and provide a 1-2 page Budget Justification of the budget items. USDA-ARS does not set a limit on the amount of indirect costs that can be allowed. However, the USWBSI’s review process will focus on the competitiveness of total project costs (direct and indirect). Because Fusarium head blight is such a severe problem for the U.S. wheat and barley industries, the USWBSI encourages institutions to limit indirect costs to 5%. Excessive indirect cost rates may prejudice the Steering Committee’s final funding recommendations to USDA-ARS. For ‘Integrated/Interdisciplinary Research’ projects, the total requested budget amount must be divided between the various research area components. This may be done by assigning a percentage of the budget to each of the RA components, or crafting individual budgets for each research area totaling the requested amount. Tuition remission is permitted under USDA-ARS extramural agreements with any type of recipient organization (i.e. universities). If tuition remission is budgeted as a direct cost, it should be included in the "Other Direct Costs" category of the Budget Page (Section H). Performance Reports If you received funding in FY05 (approximately May 2005 through April 2006), attach your entire FY05 Final Performance Report. Required even if not relevant to proposed project pre-proposal.



ON-LINE SUBMISSION PROCESS (CBCC PRE-PROPOSALS ONLY) As stated previously, the USWBSI is in the process of developing a Web-based pre-proposal submission process. For FY07, a beta or test version will be utilized for pre-proposals submitted to the Chemical, Biological and Cultural Control research area. The instructions included in this application are also relevant to the on-line submission process. Pre-proposals will be submitted on-line using web-based forms and the uploading of PDF files. Although we are still in the early stages of development, the list below outlines sections of the FY07 RFP that will be submitted via forms or PDF files:

Web-based Forms Upload of PDF Files Cover Page Project Summary Project Objectives/Research Priorities Form Current and Pending Support Form (PI and Co-PIs each will complete separate form) Single Project Budget Page Budget Justification

Pre-Proposal Body – Contains the following: • Summary of Progress for Ongoing Related

Project(s) • Project Description • References to Project Description • Facilities and Equipment • Collaborative Arrangements (includes letters

of intent from collaborators.) Vita and Publications (PI and Co-PIs) FY05 Final Performance Report (uploaded to pre-proposal by USWBSI/NFO administrator)

The on-line submission process begins with the completion of a ‘Pre-Registration’ form. PIs must complete the ‘Pre-Registration’ no later than August 30, 2006, 5:00 PM (EDT). Information from this form will be used to setup PI-based accounts. From these accounts, the PI will be able to access the on-line submission process for his/her CBCC pre-proposal(s). Limited access will be given to Co-PIs so who must indicate their agreement to assist with the facilitation of the proposed research. Access will also be given to Authorized Organizational Representatives (i.e. institution’s office which handles grants) in order to approve the budget and budget justification. We estimate that the on-line pre-proposal submission process will be operational by September 6, 2006. Detailed instructions will be distributed via e-mail to all PIs who pre-registered by the deadline (August 30, 2006). URL for accessing the Pre-Registration: http://www.scabusa.org/cgi-bin/pre_req.cgi



Check-list and Order of Required Items for

Project Pre-Proposals

Check-list of Required Items for Pre-Proposal (completed and signed) Application for Funding Cover Page– PI and Co-Investigators signature(s) required (See page 1 for guidelines on faxed signatures.) Project Summary Page (must fit within text box) Summary of Progress for Previously Funded Project – FY06 (one page maximum) Project Description (five pages or less written text; ten pages or less in total including figures and tables) References to Project Description

Project Objectives/Research Area Priorities Form Facilities and Equipment Collaborative Arrangements (must include letters of intent from each collaborator) Vita and Publication List (maximum of four pages total per scientist) Current and Pending Support (CPS) Page (complete one CPS per scientist) Budget Justification (1-2 pages maximum) Single Project Pre-Proposal Budget Page – PI Signature required FY05 Final Performance Report (include entire report if received funding in FY05)

Pre-Registration Deadline (CBCC Pre-Proposals only): Wednesday, August 30, 2006, 5:00 PM (EDT). Submission Deadline: Tuesday, September 19, 2006, 5:00 PM (EDT).

RA PD-RP FY07 USWBSI Project Pre-Proposal


CHEMICAL, BIOLOGICAL AND CULTURAL CONTROL (CBCC)

FY07 Program Description: The Chemical, Biological and Cultural Control (CBCC) research area supports research to develop effective and economical disease management practices that reduce FHB severity and the level of DON in harvested grain to meet the immediate and long-term needs of wheat and barley growers for managing FHB. Practical outcomes of research in this area include: 1) The advancement of chemical and biological agents capable of suppressing or controlling Fusarium head blight on wheat (all classes) and barley; 2) Development of BCAs to prove efficacy against Fusarium head blight; 3) Formulated BCAs ready to market to producers; 4) Improved ground and aerial application technologies that enhance the efficacy of fungicides against FHB compared with standard commercial application practices; 5) Integrated disease management programs that enhance disease suppression over any one method of disease control. This research area involves tillage practices, crop sequences, and other cultural practices; fungicides, biological control agents, and application techniques for those chemical and biological agents. Cultural control studies might include studies examining the influence of residue on disease development or other studies integrating disease management strategies. Fungicide efforts are focused on uniform field tests of the most promising fungicides on wheat, barley, and durum across multiple environments. The biological control aspect includes greenhouse and field testing of biological control agents (BCAs) that have been shown to be effective against FHB in preliminary tests, uniform field tests of the most promising BCAs in uniform tests across multiple environments, and formulation of BCAs that have commercial potential. The application portion involves comparison and modification of ground and aerial application equipment to determine the most effective methods for applying fungicides and BCAs and research on timing of applications, adjuvants, etc., that may enhance the efficacy of fungicides and BCAs. FY07 Research Priorities: The Chemical, Biological and Cultural Control research area of the U.S. Wheat & Barley Scab Initiative (USWBSI) supports research efforts addressing one or more of the following priorities:

Uniform Tests: o Collaborative efforts to evaluate advanced fungicide treatments in solo and as tank mixes of

efficacious active ingredients for effective and consistent performance against FHB of wheat (all classes) and barley across multiple environments.

o Collaborative efforts to evaluate advanced biological control agent (BCA) treatments for effective and consistent performance against FHB of wheat (all classes) and barley across multiple environments and relative to chemical standards with the most efficacious agents advanced to the chemical trial.

Biocontrol: o Discovery, Development and Evaluation: Discovery, development, optimization and

greenhouse/field evaluations of BCAs and BCA formulations in preliminary tests to provide promising agents for advanced screening.

o Biocontrol Advancement: Formulation and evaluation of formulated BCAs that were proven to be comparable in efficacy to currently available commercial fungicides in collaborative, uniform field evaluations.



o Biocontrol Ecology: Population levels required for efficacy; environmental requirements for survival

and growth; mechanisms of action against the target pathogen and environmental requirements for survival, growth, and secondary metabolism.

Application Technology: Development and evaluation of ground or aerial application technologies

that enhance the efficacy of fungicides and biological control agents against FHB compared with standard commercial application practices. Priority will be given to collaborative, interdisciplinary proposals using field-scale equipment. Ascertain an understanding of the deposition of products relative to efficacy including the distribution of the product on the head, mobility of products in the plant and interaction of droplets with head morphology, and developed explanations for the mechanisms for the increased activity associated with specific application technologies.

Integrated Disease Management: Evaluation of methods and strategies for disease management

including determination of the influence of modern crop management practices on disease development and selection of the best disease control systems that can be used in concert. These methodologies may include, but not be limited to variety selection, tillage practices, crop rotations, and chemical/biological control inputs.

Deliverables: Developing multi-state deliverables that describe effects of control practices and

technologies are encouraged. These deliverables may include traditional print media and electronic media. Deliverables should target end-user audiences in the most effective known delivery styles to reach that audience and involve communications and publications specialists in their development.



ETIOLOGY, EPIDEMIOLOGY AND DISEASE FORECASTING (EEDF) FY07 Program Description: The Etiology, Epidemiology and Disease Forecasting research area supports studies on the relationship of causal organisms with the host at the cellular, tissue, whole organism (the plant), and population levels. Practical outcomes of research in this area are disease management decision tools, and advanced knowledge pertaining to disease development. Research at the cellular, tissue and whole organism level will address host pathogen interactions that elucidate the cause and progress of disease in the host, and factors that condition the resistance/susceptibility response. In addition, research at the whole plant level will focus on an understanding of the biology and development of the pathogens in relation to the host plants and may include studies of the physical, structural and chemical (including mycotoxin) components of disease development; and of host, pathogen and environment interactions. At the population level, research will promote an understanding of epidemic development through examination of pathogen biology and environmental influences. The RA also supports studies on the refinement and deployment of disease prediction and forecasting models. FY07 Research Priorities: The Etiology, Epidemiology and Disease Forecasting research area of the U.S. Wheat & Barley Scab Initiative (USWBSI) supports research efforts addressing one or more of the following priorities:

Pathogen Biology and Epidemiology: Determination of the environmental conditions that favor pathogen survival, inoculum production, dispersal, infection, colonization, and mycotoxin production. The investigation and quantification of factors that increase the risk of severe epidemics; factors affecting temporal and spatial development of disease epidemics; and the development and delivery of disease forecasting/risk assessment systems.

Host-Parasite Interactions: Investigations of the infection and colonization process including

interactions between the fungus and susceptible/resistant wheat or barley varieties; and mycotoxin production and impact on pathogen colonization. Research addressing factors associated with high levels of mycotoxin in asymptomatic grain. Research should address cellular, tissue, whole organism (plant or fungus) and/or population levels.



FOOD SAFETY, TOXICOLOGY AND UTILIZATION OF MYCOTOXIN-CONTAMINATED GRAIN (FSTU)

FY07 Program Description: The Food Safety, Toxicology and Utilization of Mycotoxin-contaminated Grain (FSTU) research area supports research on food safety and food processing issues related to the presence of Fusarium spp. mycotoxins in wheat and barley grain. Practical outcomes of research in this area include: 1) improved toxicological data to assure that current guidelines are providing the appropriate safety factors for the consumer; 2) analytical tools that can be used by small grain producers, elevators, millers, and processors, to rapidly and reliably identify mycotoxin-contaminated grain; 3) develop appropriate strategies to deal with contaminated grain; and 4) diagnostic data on Fusarium spp. mycotoxins required for development of FHB resistant/tolerant varieties of wheat and barley. This research area supports ongoing centralized Fusarium spp. toxins diagnostic services for all USWBSI research projects. FY07 Research Priorities: The Food Safety, Toxicology and Utilization of Mycotoxin-contaminated Grain (FSTU) research area of the U.S. Wheat & Barley Scab Initiative (USWBSI) supports research efforts addressing one or more of the following priorities: Toxicology

Characterization of dose response and 'No Adverse Effect' levels for acute and chronic trichothecene oral and inhalation exposure in experimental animal models.

Identification of biomarkers for trichothecene exposure in animal models that are predictive of

adverse effects.

Correlation of animal model trichothecene toxicity studies to human cell cultures. Analytical Methods

New instrumental methods that significantly improve efficiency, selectivity and sensitivity in detection of trichothecene mycotoxins in grain, foods, and processed grain byproducts.

Novel bioanalytical methods such as immunoassay or PCR that offer significant improvements in

the quantitation or detection of Fusarium graminearum or its trichothecene mycotoxins.



GENETIC ENGINEERING AND TRANSFORMATION (GET)

FY07 Program Description: The Genetic Engineering and Transformation (GET) research area (RA) will focus primarily on development of transgenic wheat and barley with resistance to FHB. Practical outcomes of research in this area are FHB-resistant transgenic plants and technologies for rapid deployment of engineered resistance genes by breeders. Genetic transformation will be for the purpose of rapidly producing FHB-resistant, commercially viable cultivars. Therefore, proposals should address success limiting issues; examples include the use of elite cultivars or breeding lines, testing of transgene expression in stable transformants, prior testing for effectiveness against F. graminearum prior to transformation (where appropriate), the rationale of defense strategy and why it is expected to work in the field, and field testing for FHB resistance. Science and affiliated technologies will focus on host plant resistance and will involve the introduction of non-host genes, altered/enhanced expression of host resistance genes, gene silencing and pyramiding of genes with demonstrated activity against F. graminearum in the field. Antifungal genes previously shown to be effective against F. graminearum in wheat, barley or other systems, including genes identified by other disciplines such as map-based cloning or epidemiology studies, will be utilized. Transformation of non-cereal systems will be supported for the purpose of rapidly screening potential anti-Fusarium genes. FY07 Research Priorities: The Genetic Engineering and Transformation research area of the U.S. Wheat & Barley Scab Initiative (USWBSI) supports research efforts addressing one or more of the following priorities:

Transform wheat, barley, and durum to demonstrate the effectiveness of anti-Fusarium transgenes to limit Fusarium infection and early stages of growth and spread. Early testing may be done in vitro, in growth chambers, and in greenhouses. Definitive field testing must be conducted as early as possible. Priority will be given to strong anti-Fusarium gene candidates identified in other projects (e.g., protein screening, QTLs, microarrays). o Where possible, use modern wheat, barley and durum commercial cultivars for more realistic

field evaluations and better germplasm for use by plant breeders. o Proposals should include realistic methods to detect and measure levels of antifungal protein

encoded by transgenes. (This may not be possible for low-abundance proteins, such as transcription factors.)

o Develop new transformation technologies or strategies with specific application to Fusarium resistance.

o Transform with antisense or RNAi constructs designed to eliminate expression of host genes that facilitate infection or Fusarium genes necessary for infection.

o Transform with genes that enhance signaling networks identified in the resistance response.

Develop methods/systems for rapid screening (e.g., transient expression) of potentially useful antifungal genes in wheat, barley or Durum.

Identify targeting sequences (promoters and signal sequences) to express transgenes in specific

spike tissues and/or subcellular compartments where hyphae grow during earlier stages of infection. o Develop Fusarium-inducible tissue-specific promoters.



Develop strategies to enhance acceptance of Fusarium resistant transformants. Where possible, develop "like into like" (e.g., barley genes re-expressed in barley) strategies, cereal into cereal, crop plant into crop plant, etc. Reduce integrated vector sequences. Eliminate possibilities for spreading transgene to non-target plants.

Wide species testing is still encouraged in order to rapidly discover strategies for developing

resistance.



HOST GENETIC RESOURCES (HGR) FY07 Program Description: The Host Genetic Resources (HGR) research area (RA) will focus on the assembly of effective and useful native host plant resistant genes in parent stocks of sufficient adaptation so that they can and will be successfully exploited by breeders directly without the need of extensive pre-breeding. Practical outcomes of research in this area include the identification and characterization of novel sources of host plant resistance, and breeding parents having requisite agronomic and quality characteristics with one novel to several known and/or unique FHB resistance gene/QTL combinations. The predecessor to this research area (Germplasm Introduction and Enhancement) focused on discovery of novel host plant resistance through screening of germplasm collections. Pro-active, systematic screening of world genetic resources for genes with diverse and complementary effects remains a critical objective, particularly in barley and durum wheat. On the other hand, the Initiative’s fundamental objective of practical solutions demands aggressive pursuit of FHB resistance validation and stability over environments, proof of novelty via allelism and/or haplotype characterization versus known sources. Accordingly, this research area seeks projects targeting 1) discovery and validation, 2) test of novelty (e.g., allelism or haplotype testing), or 3) deployment of novel native host (or related species) genes or pyramiding of two or more known and/or novel FHB resistance genes/QTL. Specific priorities depend on the state of progress in each of the targeted crop species. Employment and/or development of contemporary strategies and technologies (i.e., molecular markers) are considered critical to either tests of novelty or germplasm enhancement. Note that mapping or genomic oriented studies of new resistance sources are considered and conducted through the Host Genetics and Genomics Research Area. Similarly nurseries proposed for screening FHB resistance predominantly of breeding lines, as opposed to characterization of unique germplasm lines, belong in the Variety Development and Uniform Nursery Research Area. The Initiative, through the HGR research area, also wishes to foster development of cutting edge strategies for aggressive application of molecular markers to accelerate variety development in our target crops. Accordingly, the Initiative specifically seeks competitive proposals involving the USDA-ARS Regional Cereal Genotyping centers and one or more plant breeding programs. These projects should be truly collaborative and multi-disciplinary, with high resolution, innovative strategies inclusive of clear milestones with timelines and specifically defined outcomes. Target outcomes can range from finished varieties to high value breeding parents containing one or more FHB resistance genes. Incorporation of novel genes and pyramiding of novel and/or known FHB resistance genes/QTL is a priority for such collaborative research with the ultimate goal of accelerating the incorporation and pyramiding of diverse FHB resistance genes into potential varieties as well as improved parental lines.



FY07 Research Priorities: The Host Genetic Resources (HGR) research area of the U.S. Wheat & Barley Scab Initiative (USWBSI) supports research efforts addressing one or more of the following priorities:

Discovery and/or introgression of novel sources of FHB resistance effective against U.S. populations of Fusarium in barley and durum wheat through systematic search, assessment and transfer of FHB resistance from cultivars, breeding lines, land-races, and wild species into adapted genotypes. Emphasis in common wheat is on discovery of unique and complementary sources of FHB resistance to facilitate enhancement and pyramiding of FHB resistance genes. Complementary sources should have FHB resistance conferred via mechanisms differing from those of known Type I and II sources or be governed by loci that are non-allelic or conferring significantly higher levels of resistance either alone or when combined with other known FHB resistance genes. Discovery and characterization of resistance sources within the primary gene pool of all three crops is of the highest priority, particularly in adapted native sources of common wheat. For those working in secondary and tertiary gene pools, a clear indication as to how usable germplasm will be developed and its effectiveness validated in adapted genotypes, and when it will be made available to plant breeders is critical.

Determining the novelty of new resistance genes via phenotypic differentiation on the basis of effectiveness, mode of action and mechanism of resistance, allelism tests with known FHB resistance genes, and studies of molecular marker diversity in comparison to known and validated FHB QTLs. Uniqueness of all putative new FHB resistance loci should be validated and confirmed via marker haplotype analysis in comparison to all known and validated FHB QTLs. Studies should be directed to understanding the inheritance and mechanisms of resistance and allelic relationships to known FHB loci. Note that traditional molecular mapping research proposals focusing on marker identification and QTL characterization of newly identified resistance sources should be submitted to Host Genetics and Genomics RA.

Generation of unique and adapted parental germplasm (pre-breeding). As the number and diversity of FHB resistance genes increases within barley, durum wheat, and common wheat, resources will shift from the two previous priorities to introgression of unique and effective FHB resistance genes and pyramiding of unique and/or known genes into adapted lines suitable for direct use as parents in cultivar development programs. Studies should be directed towards introgression of validated unique FHB resistance genes not previously deployed in adapted parental lines using classical (phenotypic selection) and/or molecular breeding (where markers are available) methods, and pyramiding of complementary FHB resistance genes into adapted parental lines via marker assisted selection (MAS). Projects focusing on MAS introgression and selection of single known and previously deployed FHB QTL in breeding populations should be submitted or included in proposals submitted to Variety Development and Uniform Nurseries RA.

Implementation of regional or market class collaborative research with the USDA-ARS Regional Cereal Genotyping Centers to identify and verify unique sources and QTL conferring FHB resistance via haplotyping studies and to rapidly introgress novel FHB resistance genes and pyramid previously mapped complementary FHB resistance genes into elite cultivars or parental lines via MAS. Studies in this area should emphasize high throughput genotyping, rapid generation advance, and strong field validation of FHB resistance as critical components of an aggressive, cutting edge scientific project with accelerated delivery of practical outcomes.



HOST GENETICS AND GENOMICS (HGG) FY07 Program Description: The Host Genetics and Genomics (HGG) research area (RA) will focus on understanding the genetic/molecular basis of native host plant resistance. Practical outcomes of research in this area are science and technologies which inform and accelerate pre-breeding and breeding, and novel candidate genes for transformation leading to commercial, FHB resistant varieties. This research area includes all elements of the former Biotechnology research area except those related to genetic engineering and transformation. This area also includes genetic studies of host resistance (except allelism testing), and marker validation studies previously covered in other areas. Research in this area includes: 1) genetic (classical or molecular), genomic (structural, functional, or comparative), or proteomic studies leading to discovery and/or identification of diagnostic markers for, and/or the nucleotide sequence of genes conferring native host plant resistance; 2) studies of mechanisms of resistance/susceptibility with the aim of facilitating efforts to map and manipulate relevant genes, or identification of candidates for transgenic approaches to resistance, and 3) adaptive research which leads to novel ‘breeder-friendly’ (low cost, high throughput, etc.) diagnostic markers. Research activities employing transformation should be submitted to the Genetic Engineering and Transformation (GET) research area (RA) as stand alone proposals. FY07 Research Priorities: The Host Genetics and Genomics (HGG) research area of the U.S. Wheat & Barley Scab Initiative (USWBSI) supports research efforts addressing one or more of the following priorities: Mapping

Genetic analysis and mapping (using molecular markers and/or physically map) of new and/or novel FHB resistance in wheat and barley germplasms, including unique types of resistance, as well as new genes for type II resistance.

Develop user-friendly markers and other techniques which accelerate gene deployment via MAS.

Validate FHB resistance QTL/markers.

Determine the durability and level of enhanced resistance when different sources and mechanisms of

FHB resistance are combined into the host genotype.

Develop BAC contigs and saturation maps for important QTL regions to facilitate MAS and map-based cloning efforts.

Gene Discovery, Mechanisms of Resistance

Identify genes encoding effective anti-Fusarium proteins.

Select mutants for enhanced resistance and enhanced susceptibility to help identify genes involved in FHB resistance.



Characterize molecular mechanisms of host–pathogen interactions as it relates to the host to identify potential resistance genes. Identify potential candidate genes involved in FHB resistance, and characterize the function of a specific candidate gene.

Determine signaling pathways that could be used to enhance host resistance or that are affected by

Fusarium during infection.



PATHOGEN GENETICS AND GENOMICS (PGG)

FY07 Program Description: The Pathogen Genetics and Genomics (PGG) research area (RA) supports studies on the genetics (classical, molecular, or population), genomics (structural, functional, or comparative), or proteomics of the causal organisms. Practical outcomes of research in this area include: 1) greater understanding of pathogen diversity and taxonomy which may inform grain crop breeding strategies and phytosanitary issues; and 2) increased understanding of pathogen biology that could lead to identification of innovative control strategies. These strategies could be implemented through novel types of resistant varieties, transgenic varieties, fungicides, biological controls, or cultural practices. Research in this area includes studies that address pathogen diversity, taxonomy, life cycle functions, mycotoxin biosynthesis, and host/parasite interactions at the molecular level. Research should improve our fundamental understanding of the evolution, ecology, reproductive biology, pathogenicity and aggressiveness, and mycotoxin production of the pathogens. Basic information on pathogen gene regulation, developmental or metabolic pathways, or virulence mechanisms may be useful for developing novel control strategies. This research area also supports studies of the effects of host resistance mechanisms on the pathogen at the molecular level. FY07 Research Priorities: The Pathogen Genetics and Genomics (PGG) research area of the U.S. Wheat & Barley Scab Initiative (USWBSI) supports research efforts addressing one or more of the following priorities:

Develop novel control methods based on pathogen genetic and genomic information. Obtain data to test feasibility or demonstrate the concept with the long-term goal of practical implementation for disease control.

Characterize FHB pathogen diversity, phylogenetics, or taxonomy. Identify aspects that may

affect disease severity, mycotoxin contamination, pathogen ecology, pathogen evolutionary potential, stability of host resistance, or phytosanitary issues.

Characterize the genomes of FHB pathogens. Identify unique or conserved adaptive features and

important genes or gene families that correlate with the pathogenic lifestyle.

Identify and characterize genes that control important pathogen traits, including pathogenicity, aggressiveness, mycotoxin production, sexual and asexual reproduction, environmental persistence, and dispersal.

Identify and characterize important pathogen gene and protein expression profiles, regulatory

networks, and developmental or metabolic pathways. Determine patterns of gene expression and protein accumulation vital to pathogenic adaptation as well as signaling, regulatory, and developmental programs essential for disease expression.

Characterize host-pathogen interactions at the molecular level. Identify infection and

colonization processes that are affected by host resistance. Identify pathogen molecular features that may serve as effectors of host defense responses, as targets of defense responses, or as countermeasures against host defense responses.

Investigators are strongly encouraged to describe how the proposed research could lead to practical solutions to problems caused by FHB.



VARIETY DEVELOPMENT AND UNIFORM NURSERIES (VDUN) FY07 Program Description: The Variety Development and Uniform Nurseries (VDUN) research area (RA) will focus on wheat and barley plant breeding programs in FHB-threatened states. Commercially viable varieties that minimize economic loss from FHB are recognized by the USWBSI as an essential and critical research objective. Practical outcomes of research in this area are new, FHB resistant varieties. Research in this area utilizes classical and emerging breeding technologies such as use of markers to confirm the presence of specific FHB resistance genes in advanced breeding lines on track for variety release. Projects should be multi-disciplinary, with high resolution, innovative strategies inclusive of clear milestones and timelines from which progress can subsequently be judged. Projects should also demonstrate awareness of developments in complementary research areas of the Initiative. Regional uniform testing nurseries will be conducted to verify putative resistance in multiple environments and promote access to diverse germplasm that is essential for efficient variety development. Projects are expected to participate in their regional uniform testing program. FY07 Research Priorities: The Variety Development and Uniform Nurseries (VDUN)) research area of the U.S. Wheat & Barley Scab Initiative (USWBSI) supports research efforts addressing one or more of the following priorities:

The breeding and release of FHB-resistant wheat and barley varieties and germplasm that are adapted to FHB-threatened states.

Multi-location validation of FHB resistance through participation in the appropriate uniform FHB screening

nurseries.

Improvement and evaluation of selection methods designed to minimize the time to delivery of FHB resistant barley and wheat varieties.

Enhancement of accuracy and consistency of FHB resistance phenotyping.

Provide growers with accurate FHB ratings of current varieties.

The delivery of new FHB-resistant wheat and barley varieties, and germplasm, is the overarching objective of this research area, and ancillary research should clearly support that objective. Priority will be given to those proposals that clearly delineate research methods, timetables, and expectations for deliverables. Successful pre-proposals should incorporate FHB breeding research into an overall breeding context that gives appropriate weight to yield, end use quality and resistance to other pests and stresses.

FY07 FORMS FY07 USWBSI Project Pre-Proposal


Check-list for Required Items This Check-list must be completed, signed and submitted with your FY07 Pre-proposal. By signing this form, you have indicated that all required items are included in your submitted pre-proposal. Pre-proposals that are submitted which are not accompanied by this form may be returned.

1. Cover Page of Application

2. Project Summary Page

3. Summary of Progress for Previously Funded Project (if applicable – i.e. received funding in FY06)

4. Project Description (limit 5 pages for text; 10 pages total)

5. References to Project Description

6. Project Objectives/RA Research Priorities Form

7. Facilities and Equipment

8. Collaborative Arrangements including letters (can be in e-mail format) from collaborators describing the services that he/she will provide.

9. Vita and Publication List (required for all investigators)

10. Current and Pending Support (CPS) Page (one per investigator)

11. Budget Justification

12. Single Project Pre-Proposal Budget Page

13. FY05 Final Performance Report (if applicable) If item is not applicable (e.g. items 3 or 12), enter N/A. _____________________________________________________________________________ PI’s Printed Name Signature Date



This form is a modified version of the CSREES-661 Form. 1IDC rate’ refers to Indirect Cost Rate or Overhead Rate (Not applicable for USDA-ARS PIs).

COVER PAGE U.S. WHEAT & BARLEY SCAB INITIATIVE FY07 PROJECT PRE-PROPOSAL APPLICATION FOR FUNDING

4. a. PHONE NUMBER

b. FAX NUMBER

c. E-MAIL ADDRESS

1. LEGAL NAME OF ORGANIZATION TO WHICH AWARD SHOULD BE

MADE

3. NAME OF AUTHORIZED

ORGANIZATIONAL REPRESENTATIVE

2. ADDRESS (Give complete mailing address and Zip Code-including County)

5. ADDRESS OF AUTHORIZED ORGANIZATIONAL REPRESENTATIVE (If different from Item 2.)

6. TITLE OF PROPOSED PROJECT (80-character Maximum, including spaces)

7. APPROXIMATE PERIOD OF PROPOSED PROJECT DATES

MAY 2007 – APRIL 2008

8. DURATION REQUESTED

12 MONTHS

9. TOTAL FUNDS REQUESTED $ IDC Rate %1

10. PRINCIPAL INVESTIGATOR AND CO-INVESTIGATOR (S)

11. a. PI’S PHONE UMBER:

a. Name of Principal Investigator (First, Middle, Last)

b. FAX NUMBER:

c. E-MAIL ADDRESS:

b. Name of Co-Investigator #1 (First, Middle, Last)

c. Name of Co-Investigator #2 (First, Middle, Last)

12. PI’s BUSINESS ADDRESS (Include Department/Zip Code) ALTERNATE SHIPPING ADDRESS (i.e. FEDEX)

15. WILL THIS PROJECT BE SENT OR HAS IT BEEN SENT TO OTHER FUNDING AGENCIES, INCLUDING OTHER USDA AGENCIES?

[ ] No [ ] Yes (If yes, list Agency acronym(s) & program(s) and fill in attached “Current and Pending” Form.)

13. LIST ALL CURRENTLY FUNDED PROJECTS IN ASSOCIATION WITH THE USWBSI

(approx. May 2006 - April 2007)

1) _________________________________________________ _________________________________________________ 2) _________________________________________________

_________________________________________________

3) _________________________________________________ _________________________________________________ 4) _________________________________________________

_________________________________________________

16. DESIGNATE WITH AN 'X' ONE RESEARCH AREA (RA) WHOSE DESCRIPTION AND PRIORITIES YOUR PROJECT IS DESIGNED TO ADDRESS. For ‘Integrated/Interdisciplinary’ Projects, list all relevant research areas. Chemical, Biological and Cultural Control (CBCC) ...................____ Etiology, Epidemiology and Disease Forecasting (EEDF) ........____ Food Safety, Toxicology, and Utilization (FSTU) ......................____ Mycotoxin-contaminated Grain Genetic Engineering and Transformation (GET) ......................____ Host Genetic Resources (HGR) ...............................................____ Host Genetics and Genomics (HGG) .......................................____ Pathogen Genetics and Genomics (PGG) ...............................____ Variety Development and Uniform Nurseries (VDUN) ..............____ Integrated/Interdisciplinary Research........................................____

List RA s (acronyms): None of the Above ....................................................................____

FOR OFFICE USE ONLY [ ]N [ ]C (______) CBCC EEDF FSTU GET HGR HGG PGG VDUN FY07- SIGNATURE OF PRINCIPAL INVESTIGATOR (All individuals listed in block 10 must sign.)

DATE

SIGNATURE OF AUTHORIZED ORGANIZATIONAL REPRESENTATIVE (Not required by USWBSI)

TITLE

DATE



PROJECT SUMMARY PAGE

Project Title: (80-chararacter Maximum)

Key Words:

(To be used by text search engines)

PROJECT SUMMARY (use minimum 12 point font and not less than single spacing)

This form is a modified version of the CSREES-1232 Form. “Summary of Progress for Ongoing Related Funded Project(s)” should follow this page (See Instructions).

Principal and Co-Investigator(s): Principal Investigator: Institution: Co-Investigator #1: Institution: Co-Investigator #2: Institution:



PROJECT OBJECTIVES/RA RESEARCH PRIORITIES FORM Instructions: Complete the table below by first filling in the box titled ‘Project Title’. This should match the title inserted in box ‘6’ of the Cover Page. Next, circle the Research Area to which this project is being submitted (for Integrated/Interdisciplinary Research (IRR) projects, circle ‘IIR’. In the left column, list separately (one objective per row), all of the project objectives. Then, in the right column titled’ RA Research Priorities’, list the appropriate research priority in the box next to the project objective to which it relates. (For IRR projects, first list the Research Area, and then the appropriate research priority.) More than one project objective may relate to the same research priority. If a project objective does not relate to specific research priorities, it must be removed from the proposed research project. Adjust size of boxes and add or delete rows as needed.

Project Title: Research Area (circle only one): CCBC EEDF FSTU GET HGR HGG PGG VDUN IIR

Project Objective(s) RA Research Priority(s) How objective relates to priority:

How objective relates to priority:






CURRENT AND PENDING SUPPORT PAGE (Each investigator must submit separate form.)

Instructions: 1. Record information for active and pending projects, including pre-proposals submitted to the USWBSI. 2. All current research to which principal investigator(s) and other senior personnel have committed a portion of their time must be listed, whether or not

salary for the person involved is included in the budgets of the various projects. 3. Provide analogous information for all proposed research which is being considered by, or which will be submitted in the near future to, other possible

sponsors including other USDA programs ant the USWBSI.

REQUESTED/AWARD AMOUNT

PRINCIPAL INVESTIGATOR

(PI)

SUPPORTING

AGENCY Total $ Amount

PI $ Allocation

EFFECTIVE

AND EXPIRATION

DATES

TITLE AND OBJECTIVES OF PROJECT Title: Current:

Objectives:

Title:

Objectives:

Title:

Objectives:

Title:

Objectives:

Title:

Objectives:

Title: Pending:

Objectives:

Title:

Objectives:

Title:

Objectives:

Title:

Objectives:

Title:

Objectives:

This form is a modified version of the CSREES-663 Form.



SINGLE FY07 PROJECT PRE-PROPOSAL BUDGET PAGE

PROJECT TITLE (Same as ‘Cover Page’):

PRINCIPAL INVESTIGATOR: FY07 Funds

Requested A. Salaries and Wages

1. No. of Senior Personnel a. PI/Co-Investigators ...................................................................................................................→ Please Note: Make sure explanation is included in ‘Budget Justification’

2. No. of Other Personnel (Non-Faculty) a. Research Associates-Post-doctorate Fellows .......................................................................→ b. Other Professionals...................................................................................................................→

c. Graduate Students.....................................................................................................................→

d. Undergraduate Students ..........................................................................................................→

e. Secretarial-Clerical ....................................................................................................................→

f. Technical, Shop and Other ......................................................................................................→

Total Salaries and Wages............................................................................................ →

B. Fringe Benefits (If charged as Direct Costs) .................................................................................. →

C. Total Salaries, Wages, and Fringe Benefits (A plus B) ............................................................. →

D. Nonexpendable Equipment (A detailed explanation listing items and dollar amounts should be included in the Budget Justification.) ......................................................................................................................................→

E. Materials and Supplies ........................................................................................................................→

YES [ ] NO [ ]

F. Travel 1. Domestic (Including Canada) ..........................................................................................................→

Do you plan to attend the 2007 National FHB Forum?

2. Foreign (List destination and amount for each trip.) ....................................................................→

G. Publication Costs/Page Charges .................................................................................................... →

H. All Other Direct Costs (A detailed explanation listing items and dollar amounts should be included in the Budget Justification.) ................................................................................................................................................→

I. Total Direct Costs (C through I) .........................................................................................................→

J. Indirect Costs If Applicable (Not applicable for PIs affiliated with ARS.) ................ IDC Rate % →

K. Total Direct and Indirect Costs (I plus J) .........................................................................................→

L. Total Amount of This Request ..........................................................................................................→ $

NAME AND TITLE (Type or Print) SIGNATURE DATE

Principal Investigator

Authorized Organizational Representative (Optional)

This form is a modified version of the CSREES-55 Form.

U.S. Wheat & Barley Scab Initiative

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