updated on 31.01.2018 NETWORK OF OFFICIAL …...updated on 31.01.2018 a.1 (54-104) a.2 (107-149) a.3...
Transcript of updated on 31.01.2018 NETWORK OF OFFICIAL …...updated on 31.01.2018 a.1 (54-104) a.2 (107-149) a.3...
updated on 31.01.2018
A.1 (54-104)
A.2 (107-149)
A.3 (152-249)
A.4 (252-302)
A.5 DVCA/DEPARTMENT OF FOOD HYGIENE (305-356)
A.6 (359-403)
A.7 (406-450)
A.8 (453-505)
A.9 (508-544)
A.10 (547-599)
A.11 (602-641)
A.12 (644-685)
DIRECTORATE OF VETERINARY CENTER OF THESSALONIKI (DVCTh)
B.1 DVCTh/DEPARTMENT OF VIRAL, RICKETTSIAL DISEASES AND TRANSMISSIBLE SPONGIFORM ENCEPHALOPATHIES (691-719)
B.2 DVCTh/DEPARTMENT OF FOOD MICROBIOLOGY, BIOCHEMICAL & PHYSICOCHEMICAL FOOD CONTROL, RESIDUES AND MARINE BIOTOXINS & TOXINS IN OTHER WATERS (722-755)
B.3 DVCTh/DEPARTMENT OF AVIAN & BEE DISEASES AND AQUATIC ORGANISMS' PATHOLOGY (758-815)
B.4 DVCTh/DEPARTMENT OF MICROBIOLOGY, INFECTIOUS DISEASES & BRUCELLOSIS (818-855)
B.5 DVCTh/DEPARTMENT: VETERINARY LABORATORY OF IOANNINA (858-902)
B.6 DVCTh/DEPARTMENT: VETERINARY LABORATORY OF KAVALA (905-955)
B.7 DVCTh/DEPARTMENT: VETERINARY LABORATORY OF SERRES (958-1023)
B.8 DVCTh/DEPARTMENT: VETERINARY LABORATORY OF LARISSA (1026-1079)
B.9 DVCTh/BIOLOGICAL PRODUCTS, DIAGNOSTIC PATHOLOGY & PARASITOLOGY OFFICE (1082-1120)
DIRECTORATE OF VETERINARY CENTER OF ATHENS (DVCA)
DVCA/DEPARTMENT OF DIAGNOSTIC PATHOLOGY, ANATOMY, HISTOLOGY & MICROBIOLOGY
DVCA/DEPARTMENT OF PARASITOLOGY-PARASITIC DISEASES, ENTOMOLOGY & BEE HEALTH
DVCA/DEPARTMENT OF MOLECULAR DIAGNOSTICS, FOOT & MOUTH DISEASE, VIRAL, RICKETTSIAL AND EXOTIC DISEASES
DVCA/DEPARTMENT OF PATHOLOGY OF AQUATIC ORGANISMS
DVCA/DEPARTMENT OF TOXICOLOGY, RESIDUES & ENVIRONMENTAL CONTAMINANTS
DVCA/DEPARTMENT: VETERINARY LABORATORY OF CHALKIS
DVCA/DEPARTMENT: VETERINARY LABORATORY OF TRIPOLIS
DVCA/DEPARTMENT: VETERINARY LABORATORY OF CHANIA
DVCA/DEPARTMENT: VETERINARY LABORATORY OF HERACLION
DVCA/DEPARTMENT: VETERINARY LABORATORY OF PATRAS
DVCA/DEPARTMENT: VETERINARY LABORATORY OF RHODES
NETWORK OF OFFICIAL LABORATORIES OF THE GENERAL DIRECTORATE OF VETERINARY SERVICES, MINISTRY OF RURAL DEVELOPMENT & FOOD
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54 A.1 DVCA/DEPARTMENT OF DIAGNOSTIC PATHOLOGY, ANATOMY, HISTOLOGY & MICROBIOLOGY55
56 25, Neapoleos Street, 153 10 Agia Paraskevi57 tel:+30 210 6399521, fax:+30 210 6399521
58 [email protected], [email protected]
60 NATIONAL REFERENCE LABORATORY
61 #62
63 OFFICIAL LABORATORY/LABORATORY DESIGNATED TO CARRY OUT ANALYSES WITHIN THE FIELD OF:
64 #
65 #
66
67 ACCREDITED TESTS PERFORMED68
69 Tested materials/ products Types of test/ Properties to be measured Applied Standards/ Techniques to be used Documentation Certificate number Valid until
70 1Blood serum originating from
ruminants
Detection of mycobacterium avium spp.
paratuberculosis antibodies
ELISA ΚΙΤ POURQUIER / IDEXX
(D07130-5)Directive64/432 ESYD 695-2 15/7/2018
71 2Blood serum samples or
plasma from ruminants
Detection of antibodies against Coxiella
burnetii (Q -fever)Indirect ELISA
ELISA Test according to the
O.I.E. Manual of Diagnostic
Tests and Vaccines for
Terrestrial Animals
(Chapter 2.1.16. -2015)
ESYD 695-2 15/7/2018
72 3
Blood serum samples of
domestic or wild ruminants,
equine, swine, camelids,
carnivores and human origin
Detection of antibodies against Βrucella
abortus/ melitensis/ suis
Rose Bengal Test (R.B.) according to the
Commission Regulation 535/2002/EC
amending Annex C of the Directive
64/432/EEC and the Decision 2000/330/EC
Directive64/432 ESYD 695-2 15/7/2018
73 4
Blood serum samples of
domestic or wild ruminants,
equine, swine, camelids,
carnivores and human origin
Detection of antibodies against Βrucella
abortus/ melitensis/ suis
Complement Fixation Test (C.F.T.) according
to the Commission Regulation 535/2002/EC
amending Annex C of the Directive
64/432/EEC and the Decision 2000/330/EC
Directive64/432 ESYD 695-2 15/7/2018
74 5Milk or blood serum samples
of domestic or wild ruminants
Detection of antibodies against Βrucella
abortus/ melitensis/ suisIndirect ELISA
ELISA Test according to the
Commission Regulation
535/2002/EC amending
Annex C of the Directive
64/432/EEC and the
ESYD 695-2 15/7/2018
75
DIRECTORATE OF VETERINARY CENTER OF ATHENS (DVCA)
Legal basis: Directive 64/432/EEC, Presidential Decree 41/2006(Α'44), Ministerial Decision 30/3430/14.01.2015(B'171)
Legal basis: Directives 64/432/EEC & 91/68/EEC, Ministerial Decisions 4224/120089/04.11.2015(B'2464) & 3339/117339/20.10.2016(B'3589)
Zoonoses & zoonotic agents (Bovine Tuberculosis)
Zoonoses & zoonotic agents (Brucelloses)
Zoonoses & zoonotic agents (Samonella)Legal basis: Regulation (EC) 2160/2003, Ministerial Decisions 349699/31.12.2009(B'2599), 133362/23.12.2011 (B' 3172), 1209/30007/13.03.2012(B'930) &
1751/51822/24.04.2013(B'1141)
Σελίδα 2 από 50
76 NON-ACCREDITED TESTS PERFORMED77
78 Tested materials/ products Types of test/ Properties to be measured Applied Standards/ Techniques to be used Documentation Comments
79 6Lymph nodes - Internal
organs (Lungs, spleen, liver ) Bovine tuberculosis
Interferon assay, tuberculin test, culture,
direct microscopy, PCR. PendingDirective 64/432
80 7Sections of small/large
intestine & mesenteric lymph
nodes - Faeces
Detection of Mycobacterium paratuberculosis Staining, microscopy OIE Terrestrial
Manual,2017 Chapter
2.1.15
81 8 Bovine Blood serum, lungs
Detection of Contagious bovine
pleuropneumonia mycoplasma (Mycoplasma
mycoides subsp. mycoides SC )
ΕLISA, Culture, PCR (upon availability of
reagents)
O.I.E. Manual of Diagnostic
Tests and Vaccines for
Terrestrial Animals
(Chapter 2.4.8. -2014).
82 9Sheep and Goat Blood serum,
milk
Detection of antibodies against Mycoplasma
agalactiaeELISA, Culture, PCR
O.I.E. Manual of Diagnostic
Tests and Vaccines for
Terrestrial Animals
(Chapter 2.7.4. -2013).
83 10 Lung tissue Detection of Mycoplasma spp Culture, stereoscopy, isolation, identification
84 11Lungs, synovial fluid , ocular
secretion
Detection of Contagious caprine
pleuropneumonia mycoplasma ( Mycoplasma
capricolum subsp.Capripneumoniae)
ΕLISA, Culture, PCR (upon availability of
reagents)
O.I.E. Manual of Diagnostic
Tests and Vaccines for
Terrestrial Animals
(Chapter 2.7.5. -2014).
85 12 Internal organsDetection of Escherichia coli, Clostridium
perfringensCulture, isolation, identification
86 13 Lung tissueDetection of Pasteurella
multocida/Mannheimia haemolyticaCulture, isolation, identification
87 14 Internal organsDetection of Staphylococcus aureus &
coagulase negative staphylococciCulture, isolation, identification
88 15 Lung tissueDetection of Pasteurella multocida /
Mannheimia haemolyticaCulture, isolation, identification
Σελίδα 3 από 50
89 16 Internal organs Detection of Streptococcus spp Culture, isolation, identification
90 17Internal organs, joints, lungs
from pigs, rabbits and
ruminants
Detection of Haemophilus spp, Βordetella spp,
Erysipelothrix spp, Culture, microscopy, isolation, identification
91 18 Internal organs Detection of Pseudomonas spp Culture, microscopy, isolation, identification
92 19 Cultures AntibiogramsAntimicrobial Susceptibility–Agar Disk
Diffusion Test
Test according to the valid
editions of CLSI:Μ2,
CLSI:M100, Eucast93 20 Internal organs Detection of moulds Culture, staining
94 21 Milk and udder secretionsDetection of Escherichia coli, Klebsiella spp,
Enterobacter spp and other
enterobacteriaceae
Culture, microscopy, biochemical testing
95 22 Milk and udder secretionsDetection of Staphylococcus aureus &
coagulase negative staphylococci Culture, microscopy, biochemical testing
96 23 Milk and udder secretionsDetection of Streptococcus agalactiae,
Streptococcus uberis, Streptococcus
dysgalactiae
Culture, microscopy, biochemical testing
97 24 Milk and udder secretions
Detection of Enterococcus spp, Proteus spp,
Pasteurella spp, Pseudomonas spp,
Mycoplasma spp, Corynebacterium spp,
Trueperella pyogenes
Culture, microscopy, biochemical testing
98 25 Milk and udder secretions Detection of yeasts Culture, microscopy, biochemical testing
99 26 Blood serum Detection of Salmonella Pullorum Culture, isolation, rapid agglutination
100 27 Feces , Blood serumDetection of antibodies against avian
mycoplasmosis (M. Gallisepticum )ELISA, rapid agglutination
101 28 Blood serumDetection of antibodies against avian
mycoplasmosis (M. synoviae ) ELISA, rapid agglutination
102 29 Yoghurt , egg shells Detection of Salmonella spp. in poultry ISO 6579:2002 ISO 6579:2002
103 30 Trachea - Lungs Detection of Mycoplasma spp in poultry Culture, microscopy
104 31 Internal organs and feces Detection of fungi in poultryCulture ( enriched substrates), microscopy,
staining105
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106
107 A.2 DVCA/DEPARTMENT OF PARASITOLOGY-PARASITIC DISEASES, ENTOMOLOGY & BEE HEALTH108
109 25, Neapoleos Street, 153 10 Agia Paraskevi110 tel:+30 210 6002217, fax:+30 210 6002217111 [email protected]
113 NATIONAL REFERENCE LABORATORY
114 #
115
116 OFFICIAL LABORATORY/LABORATORY DESIGNATED TO CARRY OUT ANALYSES WITHIN THE FIELD OF:117 #
118 #
119
120 ACCREDITED TESTS PERFORMED121
122 Tested materials/ products Types of test/ Properties to be measured Applied Standards/ Techniques to be used Documentation Certificate number Valid until
123 1 Pork meat Detection of larvae of parasite Trichinella spp Magnetic stirrer method for pooled-sample
digestion (EURL Protocol) Reg2015/1375 ESYD 695-2 15/7/2018
124 2 Dog blood serum Detection of anti-Leishmania antibodies
Indirect immunofluorescence antibody test
IFAT (IN HOUSE) according to the OIE
Terrestrial Manual (2014) Leishmaniosis
(Chapter 2.1.11)
In house method - O.I.E.
Terrestrial Manual (2014)
Leishmaniosis (Chapter
2.1.11)
ESYD 695-2 15/7/2018
125 3 Fish tissue Detection of the parasite Anisakis spp Magnetic stirrer method for pooled-sample
digestion according to EURL Protocol
Protocol according to
European Union Reference
Laboratory for parasites
(SOPs)
ESYD 695-2 15/7/2018
126
127 NON-ACCREDITED TESTS PERFORMED128
129 Tested materials/ products Types of test/ Properties to be measured Applied Standards/ Techniques to be used Documentation Comments
130 4Blood serum samples of sheep,
goats, pigs, dogs, cats, wildlifeDetection of anti-Toxoplasma antibodies
Indirect immunofluorescence antibody test
IFAT (in house)
O.I.E. Terrestrial Manual
(2017) Toxoplasmosis
(Chapter 2.9.9)
131 5Blood serum samples of
horses
Detection of anti-Trypanosoma equiperdum
antibodies
Indirect immunofluorescence antibody test
IFAT (in house)
O.I.E. Terrestrial Manual
(2013) Dourine (Chapter
2.5.3.)
132 6Blood serum samples of
horsesDetection of anti-Theileria equi antibodies
Indirect immunofluorescence antibody test
IFAT (in house)
O.I.E. Terrestrial Manual
(2014) Equine
Piroplasmosis (Chapter
2.5.8.)
133 7Blood serum samples of
horsesDetection of anti-Babesia caballi antibodies
Indirect immunofluorescence antibody test
IFAT (in house)
O.I.E. Terrestrial Manual
(2014) Equine
Piroplasmosis (Chapter
2.5.8.)
134 8
Blood serum samples of
bovine, sheep, goats, horses,
dogs
Detection of anti-Anaplasma phagocytophilum
antibodies
Indirect immunofluorescence antibody test
IFAT (in house)
O.I.E. Terrestrial Manual
(2015) Bovine
Anaplasmosis (Chapter
2.4.1.)
Parasites (Trichinella, Echinococcus, Anisakis) Legal basis: Ministerial Decision 3865/128713/22.10.2013
Honeybee health Legal basis: Ministerial Decision 4424/137557/11.11.2013(Β'2923)
Bluetongue disease surveillance program (identification of insects vectors) Legal basis: Ministerial Decision 173/5333/17.01.2017(Β'235)
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135 9 Blood serum samples of pigs Detection of anti-Trichinella spp antibodies Indirect Enzyme-linked Immunosorbent
Assay (ELISA)
Protocol developed and
validated by the European
Union Reference
Laboratory for parasites
136 10Blood serum, plasma and meat
juice of horse, pig, dogs and
wild boar
Detection of anti-Trichinella spp antibodies IndirectEnzyme-linked Immunosorbent
Assay (ELISA)Commercial kit ELISA
137 11 Trichinella larvaeIdentification of Trichinella Muscle Stage
Larvae at species level Multiplex PCR
Protocol developed and
validated by the European
Union Reference
Laboratory for parasites
138 12Intestinal mucosa of the
definitive hostDetection of Echinococcus spp. adult worms Sedimentation and Counting technique (SCT)
Protocol developed and
validated by the European
Union Reference
Laboratory for parasites
139 13Faecal smears of all animals
and birds
Detection of parasites (eggs, larvae, protozoan
oocysts or cysts etc)Microscopy/Sendimentation/ Flotation
140 14 Blood smears of all animals Detection of all parasites Microscopic examination of blood stained
with Giemsa-May Grunwald stain
O.I.E. Terrestrial Manual
(Protocol and reagents
depending on the parasite)
141 15
Faecal smears of livestock,
poultry, game birds and
companion animals
Detection of Cryptosporidium spp oocysts Acid-fast Ziehl-Neelsen stain
O.I.E. Terrestrial Manual
(May 2016)
Cryptosporidiosis (Chapter
2.9.4.)
142 16
Tissues (tongue, sceletal
muscles, peritoneal cavity,
liver, brain) of living animal
and post-mortem examination
or meat inspection of farmed
and wild animals
Diagnosis of Cysticercosis (Detection of larval
stages of cestodes-metacestodes- of Taenia
solium,T. saginata, T.ovis, T. hydatigena and T.
multiceps)
Visual inspection and microscopic
examination of cysts
O.I.E. Terrestrial Manual
(May 2014) Cysticercosis
(Chapter 2.9.5.)
143 17Individual identification of
insects
Identification of insects vectors of infectious
diseases of animals (eg. Culicoides spp,
Phlebotomine sandflies , Mosquitoes )
1. Morphological identification by
stereoscopic examination 2. Morphological
Identification of insects by microscopic
examination after preparation of slides
144 18 Abdomens of beesDetection of microsporidia Nosema spp
sporesSpore counts in haemacytometer
O.I.E. Terrestrial Manual
(May 2013) Nosemosis of
honey bees (Chapter 2.2.4.)
145 19 Bees, brood and debris Infestation of honey bees with Varroa sppExamination (morphological identification)
with the naked eye
O.I.E. Terrestrial Manual
(May 2008) Varroosis of
honey bees (Chapter 2.2.7.)
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146 20Honey bee brood, bees, hive
debris
Infestation of honey bees with mite
Tropilaelaps spp
Morphological Identification under a
microscope
O.I.E. Terrestrial Manual
(May 2017) Infestation of
honey bees with
Tropilaelaps spp (Chapter
2.2.6.)
147 21Adult Aethina tumida in
coloniesInfestation of colonies with Aethina tumida
Morphological Identification under a
stereomicroscope
O.I.E. Terrestrial Manual
(May 2017) Infestation
with Aethina tumida ( small
hive beetle) (Chapter
2.2.5.)
148 22 Larval smearsInfection of honey bees with Melissococcus
plutonius (European foolbrood)
Microscopic examination of larvae smeared
on slides and stained with Carbol fuchsin
O.I.E. Terrestrial Manual
(May 2016) European
Foolbrood of honey bees
(Infection with
Melissococcus plutonius)
(Chapter 2.2.3.)
149 23 Larval smearsInfection of honey bees with Paenibacillus
larvae (American foolbrood)
Microscopic examination of larvae smeared
on slides and stained with Gram or Carbol
fuchsin stain
O.I.E. Terrestrial Manual
(May 2016) American
Foolbrood of honey bees
(Infection with
Paenibacillus larvae)
(Chapter 2.2.2.)
150
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151
152 A.3 DVCA/DEPARTMENT OF MOLECULAR DIAGNOSTICS, FOOT & MOUTH DISEASE, VIRAL, RICKETTSIAL AND EXOTIC DISEASES153
154 25, Neapoleos Street, 153 10 Agia Paraskevi155 tel:+30 210 6007016, fax:+30 210 6082085156 [email protected]
158 NATIONAL REFERENCE LABORATORY159 #160 #161 #162 #163 #164 #165 #166 #167 #168 #169 #170 #
171 #172 #173 #174
175 OFFICIAL LABORATORY/LABORATORY DESIGNATED TO CARRY OUT ANALYSES WITHIN THE FIELD OF:176 #
177 #
178 #
179 #
180 #181
182 ACCREDITED TESTS PERFORMED183
184 Tested materials/ products Types of test/ Properties to be measured Applied Standards/ Techniques to be used Documentation Certificate number Valid until
185 1Blood serum originating from
ruminants or swine
Antibody detection against the foot-and-mouth
virus
Blocking ELISA KIT PRIOCHECK FMDV NS BY
PRIONICS (7610440)
OIE Terrestrial Manual
2017 (Chapter 2.1.8)ESYD 695-2 15/7/2018
186 2Blood serum originating from
swine
Antibody detection against the classical swine
fever virus
Blocking ELISA KIT HERDCHEK CSFV Ab BY
IDEXX (9943220)
Dir2001/89
Dec2002/106ESYD 695-2 15/7/2018
187 3Blood serum or plasma
originating from ruminants
Antibody detection against the bluetongue
virus
Competitive ELISA for bluetongue (BLUE
TONGUE COMPETITION KIT BY IDVET) (BTC-
4P)
OIE Manual of Diagnostic
Tests and Vaccines for
Terrestrial Animals 2017
(Chapter 2.1.3)
ESYD 695-2 15/7/2018
188 4Blood serum ,milk from
bovinesDetection of bovine leucosis antibodies
Elisa technique method using kit SVANOVIR
BLV-gp 51 code no10-2351-50 /10235102
OIE Terrestrial Manual
2017 (Chapter 2.4.10)ESYD 695-2 15/7/2018
189 5
Central nervous system tissue
removed from the cranium of
almost every warm-blooded
animal
Antigen detection of rabies virus Fluorescent antibody test (FAT) for rabies
OIE Manual of Diagnostic
Tests and Vaccines for
Terrestrial Animals 2017
(Chapter 2.1.17.)
ESYD 695-2 15/7/2018
190 6
Blood serum samples or
plasma or clot or filter paper
blood samples of
domesticated and wild
animals including red foxes
Antibody detection against rabies virus Blocking ELISA (BioPro Rabies ELISA Ab)
Protocols recommended by
European Union Reference
Laboratory (EURL)
ESYD 695-2 15/7/2018
191
Bluetongue disease
Epizootic haemorrhagic disease of deer
Legal basis: Directive2002/60/EC, Presidential Decrees 37/2005(Α'56), Ministerial Decision 260918/14.01.2009(Β'75)
Avian influenza
Honeybee health
Legal basis: Ministerial Decision 4411/138539/22.12.2017 (Β'4669)
Legal basis: Ministerial Decision 4424/137557/11.11.2013(Β'2923)
Legal basis:Directive 92/66/EEC, Presidential Decree 141/1996(Α'108), Ministerial Decision 279719/19.11.2004(Β'1760)
Bovine Spongiform Encephalopathy & Small ruminants Transmissible
Spongiform EncephalopathiesLegal basis: Ministerial Decisions 729/23133/01.03.2017 (B'837) & 730/23143/01.03.2017 (B'952)
Legal basis: Directives 2001/89/EC, Presidential Decrees 39/2005 (Α'57), Ministerial Decision 259/10562/30.01.2017(Β'449)
Legal basis: Directive 92/119/EEC, Presidential Decree 138/1995 (Α'88), Ministerial Decision 259/10562/30.01.2017(Β'449)
Legal basis: Directive 92/119/EEC, Presidential Decree 138/1995 (Α'88), Ministerial Decision 259/10562/30.01.2017(Β'449)
Legal basis: Directive 2000/75/EC, Presidential Decree 33/2003(Α'34), Ministerial decision 173/5333/17.01.2017(Β'235)
Legal basis: Directive 92/119/EEC, Presidential Decree 138/1995 (Α'88), Ministerial Decision 258933/05.08.2008(Β'1662)
Legal basis: Directive 2003/99/EC, Presidential Decree 41/2006(A'44), Joint Ministerial Decision 331/10301/25.01.2013(Β'198)
Legal basis: Directive 92/119/EEC, Presidential Decree 138/1995 (Α'88), Ministerial Decision 258933/05.08.2008(Β'1662)
Legal basis: Directive 92/35/EEC, Presidential Decree 33/1994(A'25)
Legal basis: Directive 92/119/EEC, Presidential Decree 138/1995 (Α'88), Ministerial Decision 258933/05.08.2008(Β'1662)
Peste des petits ruminants (PPR)
Vesicular stomatitis
Classical swine fever
Swine vesicular disease
Lumpy Skin Disease
African swine fever
Rabies
Capripoxvirus induced diseases
African horse sickness
Rift valley disease
Foot and Mouth disease
Legal basis: Ministerial Decision 27/3259/14.01.2015(Β'142)West Nile fever
Legal basis: Directive 92/119/EEC, Presidential Decree 138/1995 (Α'88), Ministerial Decision 258933/05.08.2008(Β'1662)
Legal basis: Directive 2003/85/EC, Presidential Decree 32/2007(Α'30)
Legal basis: Directive 92/119/EEC, Presidential Decree 138/1995 (Α'88), Ministerial Decision 258933/05.08.2008(Β'1662)
Legal basis: Directive 64/432/ΕEC, Ministerial Desicion 286861/31.12.2003, Circular document no 218465/17.03.2004
Rinderpest Legal basis: Directive 92/119/EEC, Presidential Decree 138/1995 (Α'88), Ministerial Decision 258933/05.08.2008(Β'1662)
Bovine leucosis
Newcastle disease
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192 NON-ACCREDITED TESTS PERFORMED193
194 Tested materials/ products Types of test/ Properties to be measured Applied Standards/ Techniques to be used Documentation Comments
195 7
Whole blood with EDTA, skin
biopsy samples and cotton
swabs of nasal, ocular or saliva
bovine samples
Detection of Lumpy Skin Disease Virus (LSDV)
and differentiation from vaccine viral strainsREAL-TIME PCR In house method Validated
196 8 Serum, Plasma
ELISA immunosorbent assay for the detection
of antibodies against Peste de Petits Ruminants
(PPR)
COMPETITIVE ELISA FOR DETECTION OF
ANTI-PPR Ab ME TO KIT ID Screen PPR
Competition ΤΗΣ IDVET
OIE Manual of Diagnostic
Tests and Vaccines for
Terrestrial Animals, 2017
Chapter 2.7.10.
197 9Blood serum from farm and
wild birds
ELISA immunosorbent assay for the detection
of antibodies against Avian Influenza Type A
virus
Kit ELISA IDEXX (Avian Influenza MultiS-
Screen) farm and wild birds
OIE Manual of Diagnostic
Tests and Vaccines for
Terrestrial Animals, 2017
Chapter 2.3.4
198 10Whole blood, bowels from
ruminantsDetection of Bluetongue virus genome Real Time PCR, conventional PCR
OIE Manual of Diagnostic
Tests and Vaccines for
Terrestrial Animals, 2017
(Chapter 2.1.3.)
Protocols recommended by
European Union Reference
Laboratory (EURL)
199 11 Equine blood plasma, serumDetection of African Equine Sickness virus
antibodiesELISA
Protocols recommended by
European Union Reference
Laboratory (EURL)
200 12 Equine whole bloodDetection of African Equine Sickness virus
genomeReal Time PCR, conventional PCR
OIE Manual of Diagnostic
Tests and Vaccines for
Terrestrial Animals, 2017
(Chapter 2.5.1.)
Protocols recommended by
European Union Reference
Laboratory (EURL)
201 13Blood plasma, serum of
ruminants and deers
Detection of Epizootic haemorrhagic disease
virus antibodiesELISA
OIE Manual of Diagnostic
Tests and Vaccines for
Terrestrial Animals, 2017
(Chapter 2.1.7.)
Protocols recommended by
European Union Reference
Laboratory (EURL)
202 14Blood plasma of ruminants
and deers
Detection of Epizootic hemorrhagic disease
viral RNAReal Time PCR
Protocols recommended by
European Union Reference
Laboratory (EURL)
203 15 Equine blood plasma, serumDetection of Equine West Nile fever virus
antibodies
West Nile competition multi-species ELISA
and West Nile IgM capture ELISA
OIE Manual of Diagnostic
Tests and Vaccines for
Terrestrial Animals, 2017
(Chapter 2.1.24.)
Protocols recommended by
European Union Reference
Laboratory (EURL)
204 16 Equine whole bloodDetection of West Nile Fever viral RNA in
equidaeReal Time PCR, conventional PCR
OIE Manual of Diagnostic
Tests and Vaccines for
Terrestrial Animals, 2017
(Chapter 2.1.24.)
Protocols recommended by
European Union Reference
Laboratory (EURL)
205 17Mandible and teeth sections of
red foxes
Tetracycline detection and red fox age
determinationMicroscopy
Protocols recommended by
European Union Reference
Laboratory (EURL)
206 18
Central nervous system (CNS)
tissue removed from the
cranium
Detection of Rabies viral RNA REAL-TIME PCR, CONVENTIONAL PCR
Protocols recommended by
European Union Reference
Laboratory (EURL)
Σελίδα 9 από 50
207 19 Equine serumDetection of Equine infectious anaemia virus
antibodiesAgar gel immunodiffusion
OIE Manual of Diagnostic
Tests and Vaccines for
Terrestrial Animals, 2017
Chapter 2.5.6.
208 20 Equidae serumDetection of antibodies against Equine
Infectious Anaemia virusELISA
OIE Manual of Diagnostic
Tests and Vaccines for
Terrestrial Animals, 2017
Chapter 2.5.6.
209 21Skin lesions, blood samples,
swabsticks
Detection of Capripoxvirus (SPPV,GTPV, LSD)
genomeReal Time PCR, conventional PCR
OIE Manual of Diagnostic
Tests and Vaccines for
Terrestrial Animals, 2017
(Chapter 2.4.13.)
Protocols recommended by
European Union Reference
Laboratory (EURL)
210 22Serum of ovine and caprine
animalsDetection of Sheep Pox virus antibodies AGAR GEL IMMUNODIFFUSION
OIE Manual of Diagnostic
Tests and Vaccines for
Terrestrial Animals, 2017
(Chapter 2.7.13.)
211 23 Skin biopsy samples Cell culture isolation of Sheep Pox virus VIRUS ISOLATION IN CELL CULTURES
OIE Manual of Diagnostic
Tests and Vaccines for
Terrestrial Animals, 2017
(Chapter 2.7.13.)
212 24Blood serum, plasma from
companion animals
Titration of antibodies against Rabies virus in
companion animals
FAVN: Fluorescent Antibody Virus
Neutralization
OIE Manual of Diagnostic
Tests and Vaccines for
Terrestrial Animals 2017
(Chapter 2.1.17)
213 25 Serum or plasma of equidaeDetection of antibodies against West Nile
Fever virus in equidae
WEST NILE COMPETITION MULTI-SPECIES
ELISA and WEST NILE IgM CAPTURE ELISA
OIE Manual of Diagnostic
Tests and Vaccines for
Terrestrial Animals, 2017
(Chapter 2.1.24.)
Protocols recommended by
European Union Reference
Laboratory (EURL)
214 26Whole blood with EDTA of
equidae
Detection of West Nile Fever viral RNA in
equidaeREAL-TIME PCR, CONVENTIONAL PCR
Protocols recommended by
European Union Reference
Laboratory (EURL)
215 27 PCR product
Sequencing of E gene & NS5, of Flavivirus &
phylogenetic analysis GEL BASED PCRs, SEQUENCING,
BIOINFORMATICS
OIE Manual of Diagnostic
Tests and Vaccines for
Terrestrial Animals, 2017
(Chapter 2.1.24.)
Protocols recommended by
European Union Reference
Laboratory (EURL)
216 28 Serum or plasma of bovidaeDetection of antibodies against Infectious
Bovine Rhinotracheitis herpesvirus ELISA
OIE Manual of Diagnostic
Tests and Vaccines for
Terrestrial Animals 2017
(Chapter 2.4.12)
217 29 Serum or plasma of bovidaeDetection of antibodies against Bovine Viral
Diarrhoea virusesELISA
OIE Manual of Diagnostic
Tests and Vaccines for
Terrestrial Animals 2017
(Chapter 2.4.7)
218 30 Equidae serumDetection of antibodies against equine arteritis
virusELISA
OIE Manual of Diagnostic
Tests and Vaccines for
Terrestrial Animals 2017
(Chapter 2.5.10)
219 31 Equidae serum
Detection of antibodies against Equine
rhinopneumonitis viruses (equidae
herpesvirus-1 and -4)
ELISA
OIE Manual of Diagnostic
Tests and Vaccines for
Terrestrial Animals 2017
(Chapter 2.5.9)
220 32 Liver of rabbitsDetection of antibodies against Rabbit
haemorrhagic disease virusELISA
OIE Manual of Diagnostic
Tests and Vaccines for
Terrestrial Animals 2017
(Chapter 2.6.2)
221 33Liver, spleen, trachea of
rabbitsDetection of Myxomatosis viral DNA CONVENTIONAL PCR
OIE Manual of Diagnostic
Tests and Vaccines for
Terrestrial Animals 2017
(Chapter 2.6.1)
222 34Serum of ovine and caprine
animals
Detection of antibodies against Caprine
arthritis-encephalitis (CAE) and maedi-visna
(MV) viruses
ELISA
OIE Manual of Diagnostic
Tests and Vaccines for
Terrestrial Animals 2017
(Chapter 2.7.2/3)
223 35 Serum of ovine
Detection of antibodies against Chlamydophila
abortus (Ovine chlamydiosis - Enzootic
Abortion of Ewes)
ELISA
OIE Manual of Diagnostic
Tests and Vaccines for
Terrestrial Animals 2017
(Chapter 2.7.6)
224 36 Fetus or aborted placentae
Detection of antibodies against Chlamydophila
abortus (Ovine chlamydiosis - Enzootic
Abortion of Ewes)
REAL-TIME PCR
OIE Manual of Diagnostic
Tests and Vaccines for
Terrestrial Animals 2017
Σελίδα 10 από 50
225 37 Fetus or aborted placentae
Detection of antibodies against Chlamydophila
abortus (Ovine chlamydiosis - Enzootic
Abortion of Ewes)
STAINING IN SMEARS
OIE Manual of Diagnostic
Tests and Vaccines for
Terrestrial Animals 2017
(Chapter 2.7.6)
226 38Serum of ovine and caprine
animals
Contagious
EcthymaAGAR GEL IMMUNODIFFUSION
OIE Manual of Diagnostic
Tests and Vaccines for
Terrestrial Animals 2017
227 39 Skin lesionsContagious
EcthymaCONVENTIONAL PCR
OIE Manual of Diagnostic
Tests and Vaccines for
Terrestrial Animals 2017
228 40 PCR productSequencing of VIR gene of ORF virus &
phylogenetic analysis
GEL BASED PCRs, SEQUENCING,
BIOINFORMATICS
OIE Manual of Diagnostic
Tests and Vaccines for
Terrestrial Animals 2017
229 41 Serum or plasma of ruminantsDetection of antibodies against Rift Valley
Fever virusELISA
OIE Manual of Diagnostic
Tests and Vaccines for
Terrestrial Animals 2017
(Chapter 2.1.18)
230 42Blood serum, plasma from
bovinesDetection of bovine leucosis antibodies
Agar gel immunodiffusion (IDEXX ΚΙΤ
POURQIER AGID LEUKOSIS)
OIE Manual of Diagnostic
Tests and Vaccines for
Terrestrial Animals 2017
(Chapter 2.4.10)
231 43Blood plasma, serum from
pigs and wildboars
Detection of Swine vesicular disease virus
antibodies
Competitive double sanwitch ELISA
(PRIONICS KIT PRIOCHECK SVDV Abs) or
Competitive ELISA for anti-SVD Abs (IDVET
KIT ID Screen Swine Vesicular Disease
Competition)
OIE Manual of Diagnostic
Tests and Vaccines for
Terrestrial Animals 2017
(Chapter 2.8.8)
232 44Brain tissue of adult
ruminantsDitection of prion protein TeSeE SAP Kits ELISA BIORAD
OIE Manual of Diagnostic
Tests and Vaccines for
Terrestrial Animals 2017
(Chapter 2.4.5) par 1.3.1233 45 Allantoic fluid from chicken
embryonated eggs
Haemagglutination test for the detection of
haemaggluitinatined factor and Haemagglutination test OIE Manual of Diagnostic
Tests and Vaccines for 234 46 Chicks (live and dead), swabs,
farm and wild birds
Detection of matrix, H5, H7 and Ν1 genes of
Avian Influenza virus Real Time PCR OIE Manual of Diagnostic
Tests and Vaccines for 235 47 Chicken embryonated eggs Avian Influenza virus isolation -inoculation of
chicken embryonated eggsVirus isolation OIE Manual of Diagnostic
Tests and Vaccines for 236 48 Blood serum from farm and
wild birds
ELISA immunosorbent assay for the detection
of antibodies against Avian Influenza Type A Kit IDEXX (Avian Influenza) chicken, turkey OIE Manual of Diagnostic
Tests and Vaccines for 237 49 Blood serum from farm and
wild birds
Haemagglutination Inhibition test for the
detection of antibodies to different subtypes of Haemagglutination Inhibition test OIE Manual of Diagnostic
Tests and Vaccines for
Σελίδα 11 από 50
238 50 PCR product
Nucleotide sequence determination of specific
regions of the genome of H5 &H7 Avian
influenza virus, cleavage site identification and
phylogenetic analysis.
Conventional PCR, Sequencing,
Bioinformatics.EURL protocols
239 51 Chicken embryonated eggsNewcastle disease virus isolation -inoculation
of chicken embryonated eggsVirus isolation
OIE Manual of Diagnostic
Tests and Vaccines for
Terrestrial Animals, 2017
Chapter 2.3.4 and EURL
protocols
240 52Allantoic fluid from chicken
embryonated eggs
Haemagglutination test for the detection of
haemaggluitinatined factor and
haemagglutination inhibition test for
characterization of Newcastle disease virus
Haemagglutination test
OIE Manual of Diagnostic
Tests and Vaccines for
Terrestrial Animals, 2017
Chapter 2.3.14 and EURL
protocols
241 53Blood serum from farm and
wild birds
Haemagglutination Inhibition test for the
detection of antibodies against Newcastle
disease virus
Haemagglutination inhibition test
OIE Manual of Diagnostic
Tests and Vaccines for
Terrestrial Animals, 2017
Chapter 2.3.14 and EURL
protocols
242 54Chicks (live and dead), swabs,
farm and wild birds
Detection of Newcastle Disease viral RNA using
Real Time PCR.Real Time PCR
OIE Manual of Diagnostic
Tests and Vaccines for
Terrestrial Animals, 2017
Chapter 2.3.14 and EURL
protocols
243 55 PCR product
Determination of the amino acid motif in 112-
117 of the fusion protein of the Newcastle
Disease virus.
Conventional PCR, Sequencing,
Bioinformatics.
OIE Manual of Diagnostic
Tests and Vaccines for
Terrestrial Animals, 2017
Chapter 2.3.14 and EURL
protocols
244 56Blood serum of chickens and
turkeys. Newcastle Disease virus antibodies detection
Kit ELISA (Newcastle Disease virus) for
chickens and turkeys.
OIE Manual of Diagnostic
Tests and Vaccines for
Terrestrial Animals, 2017
Chapter 2.3.14
245 57 Cell culture supernatant Detection of Clasical swine fever virus genome PCR
OIE Manual of Diagnostic
Tests and Vaccines for
Terrestrial Animals (ch:
2.8.3).EURL CSF SOPs.
EURL protocols
246 58EDTA BLOOD, SERUM, TISSUE
SWINE SAMPLESDetection of African swine fever virus genome REAL-TIME PCR, GEL BASED PCR
OIE Manual of Diagnostic
Tests and Vaccines for
Terrestrial Animals (ch:
2.8.1). EURL ASF SOPs.
247 59 PCR productsRabies virus nucleoprotein coding gene
sequencing and phylogenetic analysisSequencing, bioinformatic analysis EURL protocols
248 60 PCR productsSequencing of GPCR και RP030 genes of
Capripoxvirus and phylogenetic analysisSequencing, bioinformatic analysis
Published techniques
(Lamien et al 2011, Le Goff
et al 2009)
249 61 Pathologic material from
ruminants and swines
Detection of Foot and mouth disease virus
genomePCR EURL protocols
250
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251
252 A.4 DVCA/DEPARTMENT OF PATHOLOGY OF AQUATIC ORGANISMS253
254 25, Neapoleos Street, 153 10 Agia Paraskevi255 tel: +30 210 6080838, fax: +30 210 6080838256 [email protected]
258 NATIONAL REFERENCE LABORATORY259 #260 #261
262 OFFICIAL LABORATORY/LABORATORY DESIGNATED TO CARRY OUT ANALYSES WITHIN THE FIELD OF:263 #264
265 ACCREDITED TESTS PERFORMED266
267 Tested materials/ products Types of test/ Properties to be measured Applied Standards/ Techniques to be used Documentation Certificate number Valid until
268 1 Fish tissueIsolation and identification of viral
haemorragic septicaemia virus
ELISA KIT BY BIO-X DIAGNOSTICS (ΒΙΟ
Κ272/2)Dir88/2006 ESYD 695-2 15/7/2018
269 2 Fish tissueIsolation and identification of infectious
haemopoetic necrosis virus
ELISA KIT BY BIO-X DIAGNOSTICS
(BIOK273/2)Dir88/2006 ESYD 695-2 15/7/2018
270 3Fish tissue /supernatant
tissue culture
Isolation and identification of koi herpes virus
(khv)
PCR using extraction kit of QIAGEN, (69504
DN EASY)Dir88/2006 ESYD 695-2 15/7/2018
271
272 NON-ACCREDITED TESTS PERFORMED273
274 Tested materials/ products Types of test/ Properties to be measured Applied Standards/ Techniques to be used Documentation Comments
275 4Fish tissue /cell culture
supernatant
Isolation and identification of epizootic
necrosis virus
Identification with conventional PCR,
sequencing and bioinformatics analysisDir88/2006,Dec1554/2015
276 5Fish tissue /cell culture
supernatant
Isolation and identification of infectious
salmon anemia virus
Identification with conventional PCR,
sequencing and bioinformatics analysisDir88/2006,Dec1554/2016
277 6 Crustaceans tissue Identification of Taura syndrome virus Identification with conventional PCR Dir88/2006,Dec1554/2017
278 7 Crustaceans tissue Identification of Yellow head syndrome virus Identification with conventional PCR Dir88/2006,Dec1554/2018
279 8 Crustaceans tissue Identification of White spot syndrome virus Identification with conventional PCR Dir88/2006,Dec1554/2019
280 9 Crustaceans tissueIdentification of Aphanomyces astaci (Crayfish
plague)Identification with conventional PCR
OIE Aquatic animal health
diagnostic manual- OIE
EURL Protocol
281 10 Bivalve molluscs tissue Identification of Bonamia exitiosa parasite Identification with conventional PCR Dir88/2006,Dec1554/2020
282 11 Bivalve molluscs tissue Identification of Perkinsus marinus parasite Identification with conventional PCR Dir88/2006,Dec1554/2021
283 12 Bivalve molluscs tissue Identification of Microcytos mackini parasite Identification with conventional PCR Dir88/2006,Dec1554/2022
284 13 Bivalve molluscs tissue Identification of Marteilia refringens parasite Identification with conventional PCR Dir88/2006,Dec1554/2023
285 14 Bivalve molluscs tissue Identification of Bonamia ostreae parasite Identification with conventional PCR Dir88/2006,Dec1554/2024
286 15Fish tissue /cell culture
supernatant
Isolation and identification of sea bass nervous
necrosis virus
Identification with indirect
immunofluorescence, identification with
conventional PCR or real-time PCR (PCR ή
qPCR)
OIE Aquatic animal health
diagnostic manual- OIE
EURL Protocol
Fish diseases
Diseases of crustaceans
Legal basis: Directive 2006/88/ΕΚ, Presidential Decree 28/2009(Α'46), Ministerial Desicion 4619/124229/07.12.2012 (Β'3434)
Legal basis: Directive 2006/88/ΕΚ, Presidential Decree 28/2009(Α'46), Ministerial Desicion 4619/124229/07.12.2012 (Β'3434)
Diseases of bivalve molluscs Legal basis: Presidential Decree 97/2017 (Α'138), Ministerial Desicion 4619/124229/07.12.2012 (Β'3434)
Σελίδα 13 από 50
287 16Fish tissue /cell culture
supernatant
Isolation and identification of red bream
iridovirusIdentification with conventional PCR
OIE Aquatic animal health
diagnostic manual- OIE
Reference Laboratory
protocol
288 17Fish tissue /cell culture
supernatant
Isolation and identification of lymphocystis
iridovirus
Identification with conventional PCR or real-
time PCR (PCRor qPCR)
Published methods
(E.J.Valverde et al. 2016,
H.Kvitt et al.2008)
289 18 Fish tissueIsolation and identification of Photobacterium
damsella subsp. Piscicida microorganism
Identification with biochemical method
(API), Identification with serological method
(agglutination), Identification with
conventional PCR or real time PCR (qPCR)
Published methods
(C.R.Osorio et al.2000,
R.Carraro et al. 2017)
290 19 Fish tissueIsolation and identification of Listonella
anguillarum microorganism
Identification with biochemical method
(API), Identification with serological method
(agglutination on plate), Identification with
conventional PCR
Published methods
(F.Crisafi et al.,2011)
291 20 Fish tissueIsolation and identification of Aeromonas
veronii biovar sobria microorganism
Identification with biochemical method
(API), Identification with conventional PCR
Published methods
(M.Smyrli , A.Prapas et al.
2017)
292 21 Fish tissueIsolation and identification of Tenacibaculum
maritimus microorganismIdentification by conventional PCR
Published methods
( R.Avendano-Herrera et al.
2006)
293 22 Fish tissueIsolation and identification of trout Yersinia
ruckeri microorganism
Identification with biochemical method
(API), Identification with conventional PCR
Published methods (J.T.
Lejeune et al. 2000)
294 23 Fish tissueIsolation and identification of trout
Lactococcus garviae microorganism
Identification with biochemical method
(API), Identification with conventional PCR
Published
methods(A.Zlotkin et
al.1998)
295 24 Fish tissueIsolation and identification of salmonid
Aeromonas salmonicida microorganism
Identification with biochemical method
(API), Identification with conventional PCR
Published methods
(H.K.Byers et al. 2002, C.
Fernandez-Alvarez et al.
2016)
296 25 Fish tissueIsolation and identification of Edwardsiella
tarda microorganism
Identification with biochemical method
(API), Identification with conventional PCR
Published methods
(P.Katharios et al. 2015)
297 26 Fish tissueIsolation and identification of Flavobacterium
psychrophilum (Rainbow trout fry syndrome)
Identification with biochemical method
(API), Identification with conventional PCR
Published methods
(T.Wiklund et al. 2000)
298 27 Fish tissueIdentification of monogenic trematoda
parasitesMicroscopy In house method
299 28 Fish tissue Identification of digenic trematoda parasites Microscopy In house method
300 29 Fish tissue Identification of crustacean parasites Microscopy In house method
301 30 Fish tissueIdentification of myxopsporea and parasite
myxosporeaMicroscopy, preparations staning In house method
302 31 Fish tissueIsolation and identification of nematode
parasites of Anisakidae familyArtificial digestion and multiplex PCR
Published methods
(B.R.Dixon,2006)303
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304
305 A.5 DVCA/DEPARTMENT OF FOOD HYGIENE306
307 25, Neapoleos Street, 153 10 Agia Paraskevi308 tel: +30 210 6399241, fax: +30 210 6399241309 [email protected]
311 NATIONAL REFERENCE LABORATORY312 #313 #314 #315
316 OFFICIAL LABORATORY/LABORATORY DESIGNATED TO CARRY OUT ANALYSES WITHIN THE FIELD OF:317 #318 #319 #320 #321 #322
323 ACCREDITED TESTS PERFORMED324
325 Tested materials/ products Types of test/ Properties to be measured Applied Standards/ Techniques to be used Documentation Certificate number Valid until
326 1 Honey Determination of diastase index AOAC 958.09:2005, revised January 2010 Dir2001/110 ESYD 05-4 5/9/2020
327 2 Honey Determination of free acidity AOAC 962.19:2005 Dir2001/110 ESYD 05-4 5/9/2020
328 3 HoneyDetermination of hydroxymethylfurfural
(HMF)AOAC 980.23:2005 Dir2001/110 ESYD 05-4 5/9/2020
329 4 Honey Pollen identification and counting MD-39 Pollen method according to
International Honey Committee
Joint Ministerial Decision
127/2004/18.02.2005
(Β΄239)
ESYD 05-4 5/9/2020
330 5 Honey Determination of electrical conductivityMD 56 Harmonised Methods of the
International Honey Commission (2009),
Method 2
Dir2001/110 ESYD 05-4 5/9/2020
331 6 Honey Determination of moistureMD 55 Harmonised Methods of the
International Honey Commission (2009),
Method 2
Dir2001/110 ESYD 05-4 5/9/2020
332 7Foods for human consumption
and animal feeding stuffsDetection of Listeria monocytogenes ISO 11290-1:2017 Reg2073/2005 ESYD 05-4 5/9/2020
333 8Foods for human consumption
and animal feeding stuffsDetection of Salmonella spp ISO 6579-1:2017 Reg2073/2005 ESYD 05-4 5/9/2020
334 9Foods for human consumption
and animal feeding stuffsDetection Vibrio parahaemolyticus ISO 21872-1: 2017 Reg2073/2005 ESYD 05-4 5/9/2020
Viral and bacteriological contamination of bivalve molluscs
Listeria monocytogenes
Coagulase positive staphylococci, including Staphylococcus aureus
Legal basis: Ministerial Decision 3865/128713/22.10.2013
Legal basis: Ministerial Decision 3865/128713/22.10.2013
Legal basis: Ministerial Decision 3865/128713/22.10.2013
Milk and milk products (criteria for raw milk)
Legal basis: Directive 2001/110/ΕC, Presidential Decree 97/2017 (Α'138), Joint Ministerial Decision 127/2004/18.02.2005 (Β΄239)
Legal basis: Regulation (EC) 1069/2011,Regulation (ΕC) 142/2011, Circular document no 226/10890/26.01.2016
Legal basis: Desicion 2013/652/ΕU, Joint Ministerial Desicion 1920/88493/22.08.2017 (Β'3225)
Legal basis: Ministerial Desicion 3908/139386/14.12.2015 (Β'2833)
Microbiological criteria for foodstuffs Legal basis: Reguation (EC)2073/2005, Presidential Decree 97/2017 (Α'138)
Pollen content and other composition criteria of honey
Animal by-products
Antimicrobial resistance
Σελίδα 15 από 50
335 10Foods for human consumption
and animal feeding stuffs
Detection of the thermotolerant
Campylobacter sppISO 10272-1:2006 Reg2073/2005 ESYD 05-4 5/9/2020
336 11Foods for human consumption
and animal feeding stuffs
Enumeration of coagulase-positive
staphylococciISO 6888-2:1999 Reg2073/2005 ESYD 05-4 5/9/2020
337 12Foods for human consumption
and animal feeding stuffsEnumeration of microorganisms at 30οC ISO 4833-1:2013 Reg2073/2005 ESYD 05-4 5/9/2020
338 13Foods for human consumption
and animal feeding stuffs
Enumeration of β-glucoronidase positive
Escherichia coliISO 16649-2:2001 Reg2073/2005 ESYD 05-4 5/9/2020
339 14Foods for human consumption
and animal feeding stuffsEnumeration of Enterobacteriaceae ISO 21528-2:2017 Reg2073/2005 ESYD 05-4 5/9/2020
340 15Foods for human consumption
and animal feeding stuffs
Enumeration of β-glucoronidase positive
Escherichia coli in live bivalve molluscs
ISO 16649-3:2015Reg2073/2005 ESYD 05-4 5/9/2020
341 16Foods for human consumption
and animal feeding stuffs
Enumeration of Listeria monocytogenesISO 11290-2:2017 Reg2073/2005 ESYD 05-4 5/9/2020
342 17Foods for human consumption
and animal feeding stuffs
Enumeration of coagulase-positive
staphylococci
ISO 6888-1:1999Reg2073/2005 ESYD 05-4 5/9/2020
343 18 Milk and milk products Count of yeast and moulds at 25 οC ISO 6611:2004 Reg2073/2005 ESYD 05-4 5/9/2020
344 19 Milk and milk productsDetection of staphylococcal enterotoxins type
SEA to SEEISO 19020:2017 Reg2073/2005 ESYD 05-4 5/9/2020
345 20 Meat and meat productsDetection of staphylococcal enterotoxins type
SEA to SEEISO 19020:2017 Reg2073/2005 ESYD 05-4 5/9/2020
346
347 NON-ACCREDITED TESTS PERFORMED348
349 Tested materials/ products Types of test/ Properties to be measured Applied Standards/ Techniques to be used Documentation Comments
350 21 Canned foods Incubation test at 30oC & 55oC APHA 1992, Compendium Methods 3rd edAPHA 1992, Compendium
Methods 3rd ed
351 22Foods for human consumption
and animal feeding stuffsEnumeration of Coliforms ISO 4832:2006 ISO 4832:2006
352 23Foods for human consumption
and animal feeding stuffs
Enumeration of Sulfite-reducing bacteria (or
clostridia)ISO 15213:2003 ISO 15213:2003
353 24Foods for human consumption
and animal feeding stuffsEnumeration of Enterococci
APHA, Compendium of Methods for the
Microbiological Examination of Foods
APHA, Compendium of
Methods for the
Microbiological
Examination of Foods
354 25Foods for human consumption
and animal feeding stuffs
Enumeration of yeasts and moulds (products
with aw>0,95)ISO 21527-1:2008 ISO 21527-1:2008
355 26Foods for human consumption
and animal feeding stuffsEnumeration of Lactic Acid Bacteria
ISO 15214:1998
ISO 7889:2003 (IDF 117: 2003)
ISO 15214:1998
ISO 7889:2003 (IDF 117:
2003)356 27 Shellfish bivalves Detection and enumeration of norovirus ISO 15216-2:2013 ISO 15216-2:2013 Validated
357
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358
359 A.6 DVCA/DEPARTMENT OF TOXICOLOGY, RESIDUES & ENVIRONMENTAL CONTAMINANTS360
361 25, Neapoleos Street, 153 10 Agia Paraskevi362 tel: +30 210 6013899, fax: +30 210 6012594363 [email protected] 364
365 NATIONAL REFERENCE LABORATORY
366
#
367
368 ACCREDITED TESTS PERFORMED369
370 Tested materials/ products Types of test/ Properties to be measured Applied Standards/ Techniques to be used Documentation Certificate number Valid until
371 1 Meat, liver and kidney Determination of cadmium (Cd) content ΜD 01 In-house method using Atomic
Absorbtion Spectrometry with Graphite
Furnace (ZET-AAS)
Reg.333/07 & Reg.1881/06 ESYD 05-4 5/9/2020
372 2 Fisheries and fishery products Determination of cadmium (Cd) content ΜD 02 In-house method using Atomic
Absorbtion Spectrometry with Graphite
Furnace (ZET-AAS)
Reg.333/07 & Reg.1881/06 ESYD 05-4 5/9/2020
373 3Fisheries and Fishery
productsDetermination of mercury (Hg) content
ΜD 06 In-house method using Atomic
Absorption Spectrometry with Flow Injection
– Mercury Hydride (FI-MH-AAS)
Reg.333/07 & Reg.1881/06 ESYD 05-4 5/9/2020
374 4 FisheriesDetermination of dyes: Malachite Green,
Leucomalachite Green, Crystal Violet,
Leucocrystal Violet, Brilliant Green
MD-21 In house LC-MS/MS method,
validated according to the requirements of
the Decision EU/657/2002.
Dec.657/2002 ESYD 05-4 5/9/2020
375 5 Animal tissues
Determination of tetracycline residues (4):
Tetracycline, Oxytetracycline,
Chlortetracycline, Doxycycline, 4-
Εpichlortetracycline
MD-04 In house method based on the
corresponding method of the European
Community Reference Laboratory ANSES
using High Performance Liquid
Chromatography -Mass Spectrometry (LC-
MS) (ESI-positive mode), validated according
to the requirements of the Decision
EU/657/2002.
Dec.657/2002
Reg.37/2010ESYD 05-4 5/9/2020
376 6 Muscular tissues
Determination of quinolone residues (7):
Ciprofloxacin, Danofloxacin, Difloxacin,
Enrofloxacin, Flumequine, Marbofloxacin,
Oxonilic acid
MD-26 In house method based on the
corresponding method of the European
Community Reference Laboratory ANSES
using High Performance Liquid
Chromatography with FLD (HPLC-FLD),
validated according to the requirements of
the Decision EU/657/2002.
Dec.657/2002
Reg.37/2010ESYD 05-4 5/9/2020
377 7 Animal tissues
Determination of sulfonamide residues (10):
Sulfadiazine, sulfathiazole, sulfamerazine,
sulfadimidine, sulfamethoxypyridazine,
sulfamonomethoxine, sulfachlorpyridazine,
sulfadoxine, sulfadimethoxine, Na-sulfaclozine
MD-18 In house method based on the
corresponding method of the European
Community Reference Laboratory ANSES
using High Performance Liquid
Chromatography with DAD (HPLC-DAD),
validated according to the requirements of
the Decision EU/657/2002.
Dec.657/2002
Reg.37/2010ESYD 05-4 5/9/2020
Residues of substance groups Α2, Α5, Α6 (chloropromazine, nitroimidazoles),
Β1 (apart from honey), Β2α, Β2b, Β2d, Β2e, Β3b, B3c, B3d, B3e of Annex I of
Directive 96/23/ECLegal basis: Desicion 98/536/ΕC
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378 8 Milk
Determination of sulfonamide residues (7):
Sulfadiazine, sulfamerazine, sulfadimidine,
sulfamethoxypyridazine, sulfachlorpyridazine,
sulfadoxine, sulfadimethoxine
MD-17 In house method based on the
corresponding method of the European
Community Reference Laboratory ANSES
using High Performance Liquid
Chromatography with Fluorescence Detector
(HPLC-FLD), validated according to the
requirements of the Decision EU/657/2002.
Dec.657/2002
Reg.37/2010ESYD 05-4 5/9/2020
379 9 Milk Determination of Cd and PbMD-05 In house method using AAS, graphite
furnace technique (ZETAAS)Reg.333/07 & Reg.1881/06 ESYD 05-4 5/9/2020
380 10 Eggs
Determination of sulfonamide residues (8):
Sulfadiazine, sulfathiazole, sulfamerazine,
sulfamethoxypyridazine, sulfamethazine,
sulfachlorpyridazine, sulfadoxine,
sulfadimethoxine
MD-31 In house method using High
Performance Liquid Chromatography –
Tandem Mass Spectrometry (LC-MS) (ESI-
positive mode), validated according to the
requirements of the Decision EU/657/2002
Dec.657/2002
Reg.37/2010ESYD 05-4 5/9/2020
381 11 Urine, water
Determination of β-antagonists residues (17):
zilpaterol, terbutalin, salbutamol, cimaterol,
cibuterol, fenoterol, ritodrin, clencyclohexerol,
clenproperol, clenbuterol, ractopamin,
isoxsuprin, tulobuterol, clenpenterol,
mabuterol, mapenterol, brombuterol
MD-29 In house method based on the
corresponding method of the European
Community Reference Laboratory BVL using
High Performance Liquid Chromatography –
Tandem Mass Spectrometry (LC-MS/MS) (ESI-
positive mode), validated according to the
requirements of the Decision EU/657/2002.
Απόφ.ΕΕ 657/2002
Οδ.96/22/ΕΚESYD 05-4 5/9/2020
382 12 Animal tissues
Determination of β-antagonists residues (17):
zilpaterol, terbutalin, salbutamol, cimaterol,
cibuterol, fenoterol, ritodrin, clencyclohexerol,
clenproperol, clenbuterol, ractopamin,
isoxsuprin, tulobuterol, clenpenterol,
mabuterol, mapenterol, brombuterol
MD-10 In house method based on the
corresponding method of the European
Community Reference Laboratory BVL using
High Performance Liquid Chromatography –
Tandem Mass Spectrometry (LC-MS/MS) (ESI-
positive mode), validated according to the
requirements of the Decision EU/657/2002.
Dec.657/2002
Dir.96/22/ΕCESYD 05-4 5/9/2020
383 13 Animal tissues
Determination of tranquilizers (antianxiety
drugs) residues (7): acetopromazine,
chloropromazine, xylazine, promazine,
propionylopromazine, carazolol, azaperone,
Azaperol
MD-03 In house method using High
Performance Liquid Chromatography –
Tandem Mass Spectrometry (LC-MS/MS),
validated according to the requirements of
the Decision EU/657/2002.
Dec.657/2002 ESYD 05-4 5/9/2020
384 14 Animal urine
Detection and determination of (6) antithyroid
drugs (thiouracil, methyl thiouracil, propyl
thiouracil, phenyl thiouracil, tapazol,
mercaptobenzimidazol)
MD-23 In house method using LC-MS/MS
after derivatization with 3-iodo-benzyl-
bromide.
Dec.657/2002 ESYD 05-4 5/9/2020
385 15Foods of animal origin and
their productsDetermination of lead (Pb) content
ΜD-08 In – house method using Atomic
Absorbtion Spectrometry with
electrothermal Graphite Furnace.
Reg.333/07 & Reg.1881/06 ESYD 05-4 5/9/2020
386 16 Thyroid and animal tissues
Detection and determination of (6) antithyroid
drugs (thiouracil, methyl thiouracil, propyl
thiouracil, phenyl thiouracil, tapazol,
mercaptobenzimidazol)
MD-32 In house method using LC-MS/MS
after derivatization with 3-iodo-benzyl-
bromide.
Dec.657/2002
Dir.96/22/ΕCESYD 05-4 5/9/2020
387 17 Meat and blood serum Determination of nitroimidazolesMD-36 In house LC/MS-MS method based on
Community Reference Laboratory BVL
method
Dec.657/2002
Dir.96/23/ΕCESYD 05-4 5/9/2020
388 18 Tissue, milk and honeyDetermination of organophosphorus pesticide
residues
MD 38 In house LC-MS/MS method according
to SANCO 12495/2011 document
Reg.396/2005,
Reg.37/2010ESYD 05-4 5/9/2020
389 19 Milk Quantitative determination of aflatoxin M1Code: MD-53 ISO 14501/IDF 171:2007
Immunoaffinity column HPLC / FLD methodDec.657/2002 ESYD 05-4 5/9/2020
390 20 Animal tissues Quantitative determination of ochratoxin AIn house validated method Code: MD-54
Immunoaffinity column HPLC / FLD methodDec.657/2002 ESYD 05-4 5/9/2020
Σελίδα 18 από 50
391 21 Milk and Muscle
Quantitative dete rmination of Avermectins
(Moxidectin, Abamectin, Emamectin,
Doramectin, Ivermectin)
Code: MD-46 In house method based on the
corresponding method of the European
Union Reference Laboratory BVL using High
Performance Liquid Chromatography with
Fluorescence Detector (HPLC-FLD), validated
according to the requirements of the
Decision EU/657/2002.
Dec.657/2002
Reg.37/2010ESYD 05-4 5/9/2020
392 22 Animal tissues and milk
Detection of antimicrobial agents (quinolones,
cephalosporins, penicillins, macrolides,
tetracyclines, aminoglycosides, sulfonamides)
Code: MD-24 In house method based on
LMV/RMV/BIO/P TC/15.AN Version 06/17-
10-2006 Guidance of Community Reference
Laboratory ANSES
Reg.37/2010 ESYD 05-4 5/9/2020
393 23 Honey 4. Determination of Cd and Pb MD-09 In house Z-ETAAS method Reg.333/07 & Reg.1881/06 ESYD 05-4 5/9/2020
394
395 NON-ACCREDITED TESTS PERFORMED396
397 Tested materials/ products Types of test/ Properties to be measured Applied Standards/ Techniques to be used Documentation Comments
398 24 Milk Determination of tetracycline residues HPLC-DADDec.657/2002
Reg.37/2010Validated
399 25 EggsDetermination of antimicrobial residues (b-
lactames, quinolones, macrolides,
sulfonamides, tetracyclines)
LC-MS/MSDec.657/2002
Reg.37/2010Validated
400 26 Tissue and MilkDetermination of antimicrobial residues (b-
lactames, quinolones, macrolides)LC-MS/MS
Dec.657/2002
Reg.37/2010Validated
401 27 Meat, Milk, Kidney Determination of aminoglycosides LC-MS/MSDec.657/2002
Reg.37/2010Validated
402 28Animal tissues (meat, kidney),
milk, fisheries, eggs
Determination of antimicrobial agent residues
(post- screening)Biosensors
Dec.657/2002
Reg.37/2010Validated
403 29Animal tissues, milk, drinking
water
Determination of β-antagonists residues
(screening)ELISA
Guidance of European
Laboratories based on Dec.
657/2002
Validated
404
Σελίδα 19 από 50
405
406 A.7 DVCA/DEPARTMENT: VETERINARY LABORATORY OF CHALKIS407
408 Pei Dokou, Chalkida. P.O. Box 19241, 34100 Chalkida409 tel: +30 2221042521, fax: +30 2221041421410 [email protected]
412 NATIONAL REFERENCE LABORATORY413 #414 #415
416 OFFICIAL LABORATORY/LABORATORY DESIGNATED TO CARRY OUT ANALYSES WITHIN THE FIELD OF:417 #418 #419 #420 #421 #422
423 ACCREDITED TESTS PERFORMED424
425 Tested materials/ products Types of test/ Properties to be measured Applied Standards/ Techniques to be used Documentation Certificate number Valid until
426 1Foodstuffs and animal feeding
stuffsDetection of Salmonella spp. ISO 6579:2002 Reg2073/2005 ESYD 477-3 17/11/2020
427 2Foodstuffs and animal feeding
stuffs
Enumeration of microorganisms by the colony
count method at 30 0 C, using the pour plate
technique
ISO 4833-1: 2013 Reg2073/2005 ESYD 477-3 17/11/2020
428 3Foodstuffs and animal feeding
stuffs
Enumeration of microorganisms by the colony
count method at 30 0 C, using the surface
plating technique
ISO 4833-2:2013 Reg2073/2005 ESYD 477-3 17/11/2020
429 4Foodstuffs and animal feeding
stuffs
Detection and enumeration of
Enterobacteriaceae, by the colony count
technique
ΙSO/TC 21528-2:2004 Reg2073/2005 ESYD 477-3 17/11/2020
430 5Live bivalve molluscs Enumeration of β-glucoronidase positive
Escherichia coli in
ISO 16649-3:2015Reg2073/2005 ESYD 477-3 17/11/2020
431 6
Animal faeces and
environmental samples from
the primary production stage
Detection of Salmonella spp. ISO 6579:2002/Amd1:2007 ANNEX D Reg2160/2003 ESYD 477-3 17/11/2020
432 7 Salmonella spp isolates Serotyping of Salmonella spp strains ISO 6579-3:2014 Reg2160/2003 ESYD 477-3 17/11/2020
433 8Salmonella spp & E.coli
isolates
Monitoring of Antimicrobial resistance of
Salmonella spp & E.coli strains with the Disk
Diffusion agar test to:Ampicillin, cefotaxime,
ceftazidime, gentamycin, nalidixic acid,
ciprofloxacin, chloramphenicol, tetracycline,
trimethoprim.
Antimicrobial Susceptibility–Agar Disk
Diffusion Test according to the valid editions
of CLSI:Μ2, CLSI:M100, Eucast Clinical
Breakpoint Table and article of Cavaco LM
and Aarestrup FM (J.Clin.
Microbiol.2009.Sep;47(9):2751-2758)
Dir2003/99/EC
Dec2007/407/ΕΚESYD 477-3 17/11/2020
434 9Salmonella spp & E.coli
isolates
Monitoring of Antimicrobial resistance of
Salmonella spp & E.coli strains with the
Minimum Inhibition Concetration (MIC) test in
microplate
Antimicrobial Susceptibility– Minimum
Inhibition Concetration (MIC) – Broth
Microdilution method using Sensititre
microplate manufactured by TREK
Diagnostic Systems, according to the valid
editions of guidelines CLSI:Μ7, CLSI:M100,
Eucast epidemiological cut off values and the
article of Cavaco LM and Aarestrup FM
(J.Clin. Microbiol. 2009.Sep;47(9):2751-
2758)
Dec2013/652 ESYD 477-3 17/11/2020
Legal basis: Reguation (EC)2073/2005, Presidential Decree 97/2017 (Α'138)
Legal basis: Regulation (EC) 1069/2011,Regulation (ΕC) 142/2011, Circular document no 226/10890/26.01.2016
Legal basis: Directive 96/23/EC, Presidential Decree 259/1998 (A'191), Approved National Residue Monitoring Plan (2017)
Legal basis: Ministerial Decision 4424/137557/11.11.2013(Β'2923)
Microbiological criteria for foodstuffs
Zoonoses & zoonotic agents (Samonella)
Antimicrobial resistance
Legal basis: Regulation (EC) 2160/2003, Presidential Decree 41/2006 (A'44), Ministerial Decisions 349699/31.12.2009(B'2599), 133362/23.12.2011 (B' 3172),
Legal basis: Directive 2003/99/EC, Ministerial Decision 3865/128713/22.10.2013, Joint Ministerial Desicion 1920/88493/22.08.2017 (Β'3225)
Viral and bacteriological contamination of bivalve molluscs
Animal by-products
Residues of substance group B1 of Annex I of Directive 96/23/EC
Honeybee health
Σελίδα 20 από 50
435 10
Animal and poultry muscular
tissues and milk of bovine,
sheep and goats
Detection of antimicrobial agents (quinolones,
cephalosporins, penicillins, macrolides,
tetracyclines, aminoglycosides, sulfonamides)
In house method (ΜΔ-07) based on
LMV/RMV/BIO/P TC/15.AN Version 06/17-
10-2006 Guidance of Community Reference
Laboratory ANSES
Dec657/2002, Reg37/2010 ESYD 477-3 17/11/2020
436 11 Meat and poultry meat Detection of Campylobacter spp. ISO 10272-1: 2006 Reg 2073/2005 ESYD 477-3 17/11/2020
437 12Campylobacter jejuni &
Campylobacter coli isolates
Monitoring of Antimicrobial resistance of
Campylobacter jejuni & Campylobacter coli
strains with the Minimum Inhibition
Concetration (MIC) test in microplate.
Antimicrobial Susceptibility– Minimum
Inhibition Concetration (MIC) – Broth
Microdilution method, using Sensititre
microplate manufactured by TREK
Diagnostic Systems, according to the valid
editions of guidelines CLSI: VET 01-A4,
VET01S, Μ7, M100, Eucast epidemiological
Dec2013/652 ESYD 477-3 17/11/2020
438
439 NON-ACCREDITED TESTS PERFORMED440
441 Tested materials/ products Types of test/ Properties to be measured Applied Standards/ Techniques to be used Documentation Comments
442 13 FoodstuffsEnumeration of β-glucoronidase positive
Escherichia coli in ISO 16649-2:2001 Reg2073/2005
443 14Apis mellifera honey bees and
brood
Detection of Varroa destructor (Varroosis)
mites and their counting
Macroscopic & microscopic examination and
identification / OIE Terrestrial Manual 2008
2.2.7
OIE Terrestrial Animal
Health Code , Article 9.6
444 15 Forager bees Apis mellifera
Detection of Nosema spp. (spores) (qualitative
determination) and their count
(quantification)
Microscopic examination, spore count on
hemocytometer and determination of mean
spore number per bee / OIE Terrestrial
Manual 2013 2.2.4
MD
4424/137557/11.11.2013
445 16 Bees of Apis melliferaDetection of Acarapis woodi mites
(Acarapisosis)
Mιcroscopic observation & identification OIE
Terrestrial Manual 2008 2.2.1.
OIE Terrestrial Animal
Health Code, Article 9.1.
446 17 Fresh meatIsolation of ESBL enzymes, AmpC and
carbapenemases-producing E. Coli strains
Protocol of DTU Food Laboratory-European
Reference Laboratory for Antimicrobial
Resistance
Dec2013/652
447 18 CaecumIsolation of ESBL enzymes, AmpC and
carbapenemases-producing E. Coli strains
Protocol of DTU Food Laboratory-European
Reference Laboratory for Antimicrobial
Resistance
Dec2013/652
448 19 CaecumDetection of Campylobacter spp (C.jejuni /
C.coli) ISO 10272-1 Dec2013/652
449 20 Cambylobacter spp strains Identification of Campylobacter spp
PCR testing protocol for the differentiation of
Cambylobacter spp isolates (C. Jejuni, C. Coli),
suggested by the European Reference
Laboratory for Antimicrobial resistance (2nd
ed, November 2013)
Dec2013/652
450 21 Enterobacteriaceae strains
Molecular testing for the detection of plasmids
mediating the transfer of colistin to
Enterobacteriaceae isolates
PCR testing protocol for the detection of
plasmids mediating the transfer of colistin to
Enterobacteriaceae isolates, suggested by
the European Reference Laboratory for
Antimicrobial resistance (2nd ed, October
2016)
Dec2013/652
451
Σελίδα 21 από 50
452
453 A.8 DVCA/DEPARTMENT: VETERINARY LABORATORY OF TRIPOLIS454
455 Pelagos of Arcadia, 22100 Tripolis
456 tel: +30 2710 226849, fax: +30 2710 232909457 [email protected]
459 NATIONAL REFERENCE LABORATORY
460 #
461
462 OFFICIAL LABORATORY/LABORATORY DESIGNATED TO CARRY OUT ANALYSES WITHIN THE FIELD OF:463 #464 #465 #466
467 ACCREDITED TESTS PERFORMED468
469 Tested materials/ products Types of test/ Properties to be measured Applied Standards/ Techniques to be used Documentation Certificate number Valid until
470 1Milk, fish, eggs, meat and
honeyDetermination of chloramphenicol content
Modified method Afssa-Fougeres: “Methode
de confirmation des residues de
chloramphenicol dans les matrices d’origine
biologiqoue par lc/ms-ms”
Dec2002/657 ESYD 465-3 3/11/2020
471 2 Eggs, meat, fish and honey Determination of nitrofurane metabolites
Modified method Afssa-Fougeres:
“Determination de residues de nitrofuranes
(metabolites) dans les tissus par LC/MS-MS”
Dec2002/657 ESYD 465-3 3/11/2020
472 3 Honey
Detection and quantification of carbamates
Aldicarb,Carbaryl , Carbofuran ,Methomyl ,
Oxamyl ,Methiocarb ,Methiocarb Sulfoxide
,Pirimicarb,Pirimicarb
Desmethyl,Fenoxycarb,Propoxur
In house LC/MS-MS method validated
according to the Decision EC 657/2002
Dec2002/657,
SANTE/11945/2015ESYD 465-3 3/11/2020
473
474 NON-ACCREDITED TESTS PERFORMED475
476 Tested materials/ products Types of test/ Properties to be measured Applied Standards/ Techniques to be used Documentation Comments
477 4 Honey
Determination of pyrethroids Tau-
FLUVALINATE, DELTAMETHRIN, Lambda-
CYHALOTHRIN, B-CYFLUTHRIN, BIFENTHRIN,
CYPERMETHRIN (mixture of isomers),
PERMETHRIN (mixture of isomers),
FENPROPATHRIN
LC-MS/MSDec2002/657,
SANTE/11945/2015Validated
478 5 Drinking Water Determination of nitrofurane metabolitesModified method Afssa-Fougeres:
“Determination de residues de nitrofuranes
(metabolites) dans les tissus par LC/MS-MS”
Dec2002/657 Validated
479 6
Blood serum samples of
domestic or wild ruminants,
equine, swine, camelids,
carnivores and human origin
Detection of antibodies against Βrucella
abortus/ melitensis/ suis
Rose Bengal Test (R.B.) according to the
Commission Regulation 535/2002/EC
amending Annex C of the Directive
64/432/EEC and the Decision 2000/330/EC
Reg535/2002 Validated
480 7
Blood serum samples of
domestic or wild ruminants,
equine, swine, camelids,
carnivores and human origin
Detection of antibodies against Βrucella
abortus/ melitensis/ suis
Complement Fixation Test (C.F.T.) according
to the Commission Regulation 535/2002/EC
amending Annex C of the Directive
64/432/EEC and the Decision 2000/330/EC
Reg535/2002
481 8Milk samples of domestic or
wild ruminants
Detection of antibodies against Βrucella
abortus/ melitensis/ suis
ELISA Test according to the Commission
Regulation 535/2002/EC amending Annex C
of the Directive 64/432/EEC and the
Decision 2000/330/EC
Reg535/2002
482 9Blood serum samples of
domestic or wild ruminants
Detection of antibodies against Βrucella
abortus/ melitensis/ suis
ELISA Test according to the Commission
Regulation 535/2002/EC amending Annex C
of the Directive 64/432/EEC and the
Decision 2000/330/EC
Reg535/2002
483 10Blood serum samples of
bovine
Detection of antibodies against Bovine Leucosis
Virus
ELISA Test according to the Commission
Directive 64/432/EC, the Decision
2009/976/ΕΕ and the OIE Manual of
Diagnostic Tests and Vaccines for Terrestrial
Dir64/432/ΕEC,
Dec2009/976/ΕU
484 11Blood serum samples of Sheep
& Goat
Detection of antibodies against Maedi Visna –
CAE virusesELISA
OIE Terrestrial Manual
2017 Chapter 2.7.2/3
485 12Blood serum samples of Sheep
& Goat
Detection of antibodies against Chlamydophila
abortus (Chlamydial Abortions in Sheep &
Goat)
ELISAOIE Terrestrial Manual
2015 Chapter 2.1.16.
486 13Blood serum samples of Sheep
& Goat
Detection of antibodies against Coxiella
burnetii (Q Fever)ELISA
OIE Terrestrial Manual
2012 Chapter 2.7.7
487 14Blood serum samples of
ruminantsDetection of antibodies Toxoplasma gondii ELISA
OIE Terrestrial Manual
2017 Chapter 2.9.9.
488 15Blood serum samples of
ruminants
Detection of antibodies Mycobacterium Avium
subsp. Paratuberculosis ELISA
OIE Terrestrial Manual
2014 Chapter 2.1.15.
Residues of substance groups Α6 (chloramphenicol, nitrofurans) and Β2c of
Annex I of Directive 96/23/ECLegal basis: Desicion 98/563/ΕC
Honeybee health
Zoonoses & zoonotic agents (Brucelloses)
Bovine leucosis
Legal basis: Directives 64/432/EEC & 91/68/EEC, Ministerial Decisions 4224/120089/04.11.2015(B'2464) & 3339/117339/20.10.2016(B'3589)
Legal basis: Directive 64/432/ΕEC, Ministerial Desicion 286861/31.12.2003, Circular document no 218465/17.03.2004
Legal basis: Ministerial Decision 4424/137557/11.11.2013(Β'2923)
Σελίδα 22 από 50
489 16Blood smears of food-
producing animalsDetection of parasites
Microscopic examination of blood stained
with Giemsa
O.I.E. Terrestrial Manual
(Protocol and reagents
depending on the parasite)
490 17Faecal smears of food-
producing animalsDetection of Cryptosporidium spp oocysts Modified Ziehl-Neelsen stain
OIE Terrestrial Manual
2016 Chapter 2.9.4.
491 18
Sections of small/large
intestine & mesenteric lymph
nodes - Faeces
Detection of Mycobacterium Avium subsp.
Paratuberculosis
Microscopic examination, Ziehl-Neelsen
Stain.
OIE Terrestrial Manual
2014 Chapter 2.1.15.
492 19
Diagnostic Specimens and
Milk Samples of food-
producing animals
Detection of Pasteurella
multocida/Mannheimia haemolytica,
Staphylococcus spp., Streptococcus spp.,
Pseudomonas spp., Escherichia coli, Klebsiella
Culture, isolation, identification.
P.J.Quin, M.E. Carter,B.K.
Markey, G.R. Carter, Clinical
Veterinary Microbiology,
WOLFE, 1994.493 20 Brain Sections Detection of Listeria monocytogenes Culture, isolation, identification. ISO 11290-1:2017
494 21Lung tissue, synovial fluid,
milk samplesDetection of Mycoplasma spp. Culture, isolation, identification.
OIE Terrestrial Manual
2013 Chapter 2.7.4.
495 22 Bacterial Pathogens Isolates
Monitoring of Antimicrobial Resistance of
Bacterial Pathogens with the Disk Diffusion
agar test.
Antimicrobial Susceptibility–Agar Disk
Diffusion Test according to the valid editions
of CLSI:Μ2, CLSI:M100, Eucast.
CLSI M2 M100 EUCAST
496 23Apis mellifera honey bees and
brood.
Diagnosis of Varroosis / Infestation with
Varroa destructorMacroscopic & Microscopic Examination
OIE Terrestrial Manual
2008 Chapter 2.2.7.
497 24 Apis mellifera honey bees Diagnosis of Nosemosis Microscopic ExaminationOIE Terrestrial Manual
2013 Chapter 2.2.4.
498 25 Apis mellifera honey beesDiagnosis of Acarapisosis / Infestation with
Acarapis WoodiMicroscopic Examination
OIE Terrestrial Manual
2008 Chapter 2.2.1.
499 26 Apis mellifera BroodDiagnosis of European Foulbrood / Infestation
with Melissococcus Plutonius Microscopic Examination, identification.
OIE Terrestrial Manual
2016 Chapter 2.2.3.
500 27 Apis mellifera BroodDiagnosis of American Foulbrood / Infestation
with Paenibacillus larvae Culture, isolation, identification.
OIE Terrestrial Manual
2016 Chapter 2.2.2.
501 28Apis mellifera honey bees and
brood.
Infestation of Honey Bees with Tropilaelaps
spp.Macroscopic & Microscopic Examination
OIE Terrestrial Manual
2017 Chapter 2.2.6.
502 29Apis mellifera honey bees,
brood and beehives.
Infestation with Aethina tumida (Small Hive
Beetle)Macroscopic Examination & Stereoscopy
OIE Terrestrial Manual
2017 Chapter 2.2.5.
503 30 Apis mellifera honey bees Infestation with Malpighamoeba mellificae Microscopic Examination Bibliography
504 31 Apis mellifera brood. Infestation with Ascosphaera apis Microscopic Examination Bibliography
505 32 Apis mellifera brood. Infestation with Aspergillus flavus Microscopic Examination Bibliography
506
Σελίδα 23 από 50
507
508 A.9 DVCA/DEPARTMENT: VETERINARY LABORATORY OF CHANIA509
510 M. Mpotsari 66, Chania, Zip . 73136, Prefecture of Chania511 tel: +30 28210 96189, fax: +30 28210 88397512 [email protected] 513
514 NATIONAL REFERENCE LABORATORY
515 #
516
517 OFFICIAL LABORATORY/LABORATORY DESIGNATED TO CARRY OUT ANALYSES WITHIN THE FIELD OF:518 #519
520 ACCREDITED TESTS PERFORMED521
522 Tested materials/ products Types of test/ Properties to be measured Applied Standards/ Techniques to be used Documentation Certificate number Valid until
523 1 HoneyDetermination of tetracyclines (ΟΤC, ΤC, CTC,
DTC)
In house Method (ΜΔ01) using HPLC
validated according to the Decision EC
657/2002
Dec2002/657 ESYD 481-4 13/11/2020
524
525 NON-ACCREDITED TESTS PERFORMED526
527 Tested materials/ products Types of test/ Properties to be measured Applied Standards/ Techniques to be used Documentation Comments
528 2 Honey Determination of streptomycine HPLC-FLD Dec2002/657 Validated
529 3 Honey Determination of sulfonamides HPLC-FLD Dec2002/657 Validated
530 4 Apis mellifera honey bees Average Nosema sp. spore count per sample Spore counts in a haemacytometer
OIE Terrestrial Manual
2014 2.2.4 /
MD
4424/137557/11.11.2013
531 5Apis mellifera honey bees and
broodVarroa destructor infestation Macroscopic & microscopic examination
OIE Terrestrial Animal
Health Code-Article 9.6
OIE Terrestrial Manual
2014 2.2.7 /
MD
4424/137557/11.11.2013
532 6 Apis mellifera honey bees Tropilaelaps sp. infestation Macroscopic & microscopic examination
OIE Terrestrial Animal
Health Code-Article 9.5
OIE Terrestrial Manual
2014 2.2.6533 7 Apis mellifera honey bees Detection of Malpighamoeba mellificae Microscopy
534 8 Honey bees, brood Detection of Paenibacillus larvae (AFB) Culture-microscopy-catalase testing
OIE Terrestrial Animal
Health Code-Article 9.2
OIE Terrestrial Manual
2014 Chapter 2.2.2
Residues of substance group Β1 of Annex I of Directive 96/23/EC in honeyLegal basis: Desicion 98/536/ΕC
Honeybee health Legal basis: Ministerial Decision 4424/137557/11.11.2013(Β'2923)
Σελίδα 24 από 50
535 9 Honey bees, brood Detection of Melissococcus plutonius (EFB) Μicroscopy
OIE Terrestrial Animal
Health Code-Article 9.3
OIE Terrestrial Manual
2014 Chapter 2.2.3536 10 Brood Detection of Ascosphaera apis Macroscopic observation & microscopy Beebook, coloss
537 11 Brood Detection of Aspergillus flavus Macroscopic observation & microscopy Beebook, coloss
538 12 Honey bees, brood, beehives Small Hive Beetle infestation Macroscopic observation & stereoscopyOIE Terrestrial Manual
2014 Chapter 2.2.5539 13 Adults, larvae Detection of Galleria mellonella Macroscopic observation Beebook, coloss
540 14 Adults, larvae Detection of Achroia grisella Macroscopic observation Beebook, coloss
541 15Sheep & goat whole blood or
blood serum
Detection of antibodies against Maedi Visna –
CAE viruses ELISA, Agar gel immunodiffusion test
O.I.E. / 4th ed.,2000
OIE Terrestrial Manual
2017 Chapter 2.7.2/3
542 16Blood serum originating from
ruminants
Detection of mycobacterium avium spp.
paratuberculosis antibodies ELISA
Directive 64/432
OIE Terrestrial Manual
2014 Chapter 2.1.15
543 17 Sheep and goat milkDetection of Mycoplasma agalactiae
(Contagious Agalactia)Culture & stereoscopy
OIE Terrestrial Manual
2013, Chapter 2.7.4
544 18 Milk Mastitis testWhiteside test, culture, microscopy,
biochemical testing & antibiogram545
Σελίδα 25 από 50
546
547 A.10 DVCA/DEPARTMENT: VETERINARY LABORATORY OF HERACLION548
549 Port of Heraklio, Pier 03, Heraklion, Crete, Greece
550 tel: +30 2810280869, fax: +30 2810341743551 [email protected] 552
553 OFFICIAL LABORATORY/LABORATORY DESIGNATED TO CARRY OUT ANALYSES WITHIN THE FIELD OF:554 #555 #556 #557 #558
559 ACCREDITED TESTS PERFORMED560
561 Tested materials/ products Types of test/ Properties to be measured Applied Standards/ Techniques to be used Documentation Certificate number Valid until
562 1Foodstuffs and animal
feeding stuffsDetection of Salmonella spp. ISO 6579: 2002
Reg2073/2005
Reg1774/2002 ESYD 585-2 8/12/2017
563 2Foodstuffs and animal
feeding stuffsDetection of Listeria monocytogenes ISO 11290-1:1996/Amd1:2004
Reg2073/2005
Reg1441/2005
Reg365/2010
ESYD 585-2 8/12/2017
564 3
Blood serum samples of
domestic or wild ruminants,
equine, swine, camelids,
carnivores and human origin
Detection of antibodies against Βrucella
abortus, B. melitensis, B. suis
Rose Bengal Test (R.B.) according to the
Commission Regulation 535/2002/EC
amending Annex C of the Directive
64/432/EEC and the Decision 2000/330/EC
Dir64/432 ESYD 585-2 8/12/2017
565
566 NON-ACCREDITED TESTS PERFORMED567
568 Tested materials/ products Types of test/ Properties to be measured Applied Standards/ Techniques to be used Documentation Comments
569 4
Animal faeces and
environmental samples from
the primary production stage
Detection of Salmonella spp. ISO 6579:2002/Amd1:2007 ANNEX D
Reg2160/2003
Reg1003/2005
Reg1168/2006
Reg646/2007
Reg213/2009
570 5
Blood serum samples of
domestic or wild ruminants,
equine, swine, camelids,
carnivores and human origin
Detection of antibodies against Βrucella
abortus/ melitensis/ suis
Complement Fixation Test (C.F.T.) according
to the Commission Regulation 535/2002/EC
amending Annex C of the Directive
64/432/EEC and the Decision 2000/330/EC
Reg2073/2005
Reg1441/2005
Reg365/2010
571 6Food and Animal feeding
stuffs
Enumeration of coagulase positive
staphylococcusISO 6888-2:1999/ Amd1: 2003
Reg2073/2005
Reg1441/2005
Reg365/2010
572 7Food and Animal feeding
stuffs
Enumeration of the total aerobic
microorganisms at 30 oC by the pour plate
technique
ISO 4833-1:2013
Reg2073/2005
Reg1441/2005
Reg365/2010
Dec1545/70158
573 8Food and Animal feeding
stuffs
Enumeration of b- glucuronidase- positive
Escherichia coli in agarISO 16649-2:2001
Reg2073/2005
Reg1441/2005
Reg365/2010
574 9Food and Animal feeding
stuffs
Detection and enumeration of
EnterobacteriacaeISO 21528-2: 2004
Reg2073/2005
Reg1441/2005
Reg365/2010
Dec1545/70158
575 10Food and Animal feeding
stuffsEnumeration of Listeria monocytogenes ISO 11290-2: 1998 / Amd 1:2004
Reg2073/2005
Reg1441/2005
Reg365/2010
576 11Food and Animal feeding
stuffs
Enumeration of the total aerobic
microorganisms at 30 oC by the surface plating
technique
ISO 4833-2:2013
Reg2073/2005
Reg1441/2005
Reg365/2010
577 12 Foodstuffs Yeast and mould count ISO 21527-1:2008Reg2073/2005
Reg1441/2005578 13 Blood serum Enzootic leucosis antibodies detection Agar gel immunodiffusion
579 14 Milk Mastitis test Culture & antibiogram
Legal basis: Directives 64/432/EEC & 91/68/EEC, Ministerial Decisions 4224/120089/04.11.2015(B'2464) & 3339/117339/20.10.2016(B'3589)
Legal basis: Directive 64/432/ΕEC, Ministerial Desicion 286861/31.12.2003, Circular document no 218465/17.03.2004
Legal basis: Reguation (EC)2073/2005, Presidential Decree 97/2017 (Α'138)
Legal basis: Ministerial Decision 4424/137557/11.11.2013(Β'2923)
Zoonoses & zoonotic agents (Brucelloses)
Bovine leucosis
Microbiological criteria for foodstuffs
Honeybee health
Σελίδα 26 από 50
580 15Apis mellifera honey bees and
broodVarroa destructor infestation
Macroscopic & microscopic examination and
characterization / OIE Terrestrial Manual
2014 2.2.7
OIE Terrestrial Animal
Health Code , Article 9.6
581 16 Apis mellifera honey bees Tropilaelaps sp. infestationMacroscopic & microscopic examination and
characterization / OIE Terrestrial Manual
2014 2.2.6
OIE Terrestrial Animal
Health Code, Article 9.5.
582 17 Apis mellifera honey bees Average Nosema sp. spore count per sampleSpore counts in a haemacytometer / OIE
Terrestrial Manual 2014 2.2.4
MD
4424/137557/11.11.2013583 18 Apis mellifera honey bees Detection of Malpighamoeba mellificae Microscopy
584 19 Honey bees, brood Detection of Melissococcus plutonius (EFB)Culture-microscopy / OIE Terrestrial Manual
2014 Chapter 2.2.3
OIE Terrestrial Animal
Health Code , Article 9.3.
585 20 Honey bees, brood Detection of Paenibacillus larvae (AFB)Culture-microscopy / OIE Terrestrial Manual
2014 Chapter 2.2.2
OIE Terrestrial Animal
Health Code , Article 9.2.
586 21 Brood Detection of Ascosphaera apis Macroscopic observation & microscopy
(Beebook, coloss)
587 22 Brood Detection of Aspergillus flavusMacroscopic observation & microscopy
(Beebook, coloss)
588 23 Honey bees, brood, beehives Small Hive Beetle infestationMacroscopic observation & stereoscopy - OIE
Terrestrial Manual 2014 2.2.5
589 24 Adult waspsDetection of Vespa velutina and other Vespidae
sp.
Macroscopic observation & stereoscopy
(EURL, method)
590 25 Adults, larvae Detection of Galleria mellonella Macroscopic observation & stereoscopy
(BeeBook, Coloss)
591 26 Adults, larvae Detection of Achroia grisellaMacroscopic observation & stereoscopy
(BeeBook, Coloss)
592 27Faecal smears of all animals
and birds
Detection of parasites (eggs, larvae, protozoan
oocysts or cysts etc)Microscopy/Sendimentation/ Flotation
593 28 Internal organs Detection of pathogens Culture, isolation, identification
594 29 Milk and udder secretionsDetection of Escherichia coli, Klebsiella spp,
Enterobacter spp and other
enterobacteriaceae
Culture, microscopy, biochemical testing
595 30 Milk and udder secretionsDetection of Staphylococcus aureus &
coagulase negative staphylococci Culture, microscopy, biochemical testing
596 31 Milk and udder secretionsDetection of Streptococcus agalactiae,
Streptococcus uberis, Streptococcus
dysgalactiae
Culture, microscopy, biochemical testing
597 32 Milk and udder secretions
Detection of Enterococcus spp, Proteus spp,
Pasteurella spp, Pseudomonas spp,
Mycoplasma spp, Corynebacterium spp,
Trueperella pyogenes
Culture, microscopy, biochemical testing
598 33 Canned foods Incubation test at 30oC & 55oC APHA 1992, Compendium Methods 3rd ed
599 34 Animal tissues and milk
Detection of antimicrobial agents (quinolones,
cephalosporins, penicillins, macrolides,
tetracyclines, aminoglycosides, sulfonamides)
Method OE 504A-12 based on the method
‘Screening Test for Antibiotic Residues-STAR
Protocol’ of the NRL-Residues
Reg470/2009
Reg37/2010
Reg758/2010
Reg759/2010
Reg84/2012 Reg86/2012
Reg931/2011
Reg726/2004600
Σελίδα 27 από 50
601
602 A.11 DVCA/DEPARTMENT: VETERINARY LABORATORY OF PATRAS603
604 15 Notara str, Patras, 26442605 tel: +30 2610 427383, fax: +30 2610 454027606 [email protected] 607
608 NATIONAL REFERENCE LABORATORY609 #610
611 OFFICIAL LABORATORY/LABORATORY DESIGNATED TO CARRY OUT ANALYSES WITHIN THE FIELD OF:612 #
613#
614 #615
616 ACCREDITED TESTS PERFORMED617
618 Tested materials/ products Types of test/ Properties to be measured Applied Standards/ Techniques to be used Documentation Certificate number Valid until
619 1
Foods in form of liquid or
powder (Cat. 1) &
Homogeneous solid food (Cat.
2)
Enumeration of the total aerobic
microorganisms at 30 oC.ISO 4833:2003 Reg2073/2005 ESYD 769-2 22/9/2019
620 2
Foods in form of liquid or
powder (Cat. 1) &
Homogeneous solid food (Cat.
2)
Detection and enumeration of
EnterobacteriaceaeISO 21528-2:2004 Reg2073/2005 ESYD 769-2 22/9/2019
621 3
Food except foods in form of
liquid or powder,
homogeneous solid food and
small solid food (Cat. 4).
Detection of Listeria monocytogenes ISO 11290-1:1996/ Amd. 1:2004) Reg2073/2005 ESYD 769-2 22/9/2019
622 4
Food except foods in form of
liquid or powder,
homogeneous solid food and
small solid food (Cat. 4)
Enumeration of the total aerobic
microorganisms at 30 oCISO 4833-1:2013 Reg2073/2005 ESYD 769-2 22/9/2019
623 5
Food except foods in form of
liquid or powder,
homogeneous solid food and
small solid food (Cat. 4)
Detection and enumeration of
EnterobacteriaceaeISO 21528-2 Reg2073/2005 ESYD 769-2 22/9/2019
624 6Homogeneous solid food (Cat.
2)Detection of Listeria monocytogenes ISO 11290-1:1997 DAM 1/2005 Reg2073/2005 ESYD 769-2 22/9/2019
625 7 Milk Determination of alkaline phosphate (ALP)Fluorimetric method (ISO/DIS 11816-1, IDF
155-1 Part 1 & 2)Reg1664/2006 ESYD 769-2 22/9/2019
626
627 NON-ACCREDITED TESTS PERFORMED
628
629 Tested materials/ products Types of test/ Properties to be measured Applied Standards/ Techniques to be used Documentation Comments
630 8Milk, fisheries, meat, honey,
eggs
Determination of chloramphenicol content
(screening)ELISA Dec2002/657
631 9 Milk Somatic cell count ISO 13366-1:2008 Reg1664/2006
632 10 Water samplesEnumeration of fecal coliforms, enterococcus,
pseudomonasMembrane Filtration MD38295/2007
633 11 Milk products Yeast and mould count ISO 6611:2004 PD267/2002634 12 Cheese Determination of alkaline phosphate (ALP) ISO/DIS 11816-2 IDF 155-2 Καν1664/2006
Milk and milk products (alkaline phosphatase activity) Legal basis: Ministerial Desicion 3865/128713/22.10.2013, Ministerial Desicion 3908/139386/14.12.2015 (Β'2833)
Milk and milk products (criteria for raw milk)
Microbiological criteria for foodstuffs
Residues of substance group A6 (chloramphenicol) of Annex I of Directive
Legal basis: Ministerial Desicion 3908/139386/14.12.2015 (Β'2833)
Legal basis: Reguation (EC)2073/2005, Presidential Decree 97/2017 (Α'138)
Legal basis: Directive 96/23/EC, Presidential Decree 259/1998 (A'191), Approved National Residue Monitoring Plan (2017)
Σελίδα 28 από 50
635
13
Food except foods in form of
liquid or powder,
homogeneous solid food and
small solid food
Detection Salmonella spp ISO/DIS 6579:2002 Reg2073/2005
636 14
Food except foods in form of
liquid or powder,
homogeneous solid food and
small solid food
Enumeration of Escherichia coli ISO/DIS 16649-2:2001 Reg2073/2005
637 15 Homogeneous solid food Detection Salmonella spp ISO/DIS 6579:2002 Reg2073/2005
638 16 Homogeneous solid food Enumeration of Escherichia coli ISO/DIS 16649-2:2001 Reg2073/2005
639 17 Foodsuffs of animal origin Enumeration of c.p. Staphylococci ISO 6888-2:1999 Reg2073/2005
640 18 Bivalve molluscs Enumeration of Escherichia coli ISO 16649-3:2005 Reg2073/2005
64119
Blood serum of small
ruminantsDetection of antibodies against B. melitensis
Rose Bengal Test (R.B.) according to
Directive 64/432/EEC (and its updates)Dir64/432
642
Σελίδα 29 από 50
643
644 A.12 DVCA/DEPARTMENT: VETERINARY LABORATORY OF RHODES645
646 4th Km Kallithea Avenue, P.C. 85100, Rhodes, Greece647 tel: +30 22410 65444, fax: +30 22410 67193648 [email protected]
650 OFFICIAL LABORATORY/LABORATORY DESIGNATED TO CARRY OUT ANALYSES WITHIN THE FIELD OF:651 #652 #653 #654 #655 #656 #657
658 ACCREDITED TESTS PERFORMED659
660 Tested materials/ products Types of test/ Properties to be measured Applied Standards/ Techniques to be used Documentation Certificate number Valid until
661 1Food and Animal feeding
stuffs (including carcasses of
cattle, sheep, goats, pigs)
Detection of Salmonella spp ISO 6579:2002 / Cor.1:2004 Reg2073/2005 ESYD 475-3 16/11/2020
662 2Food and Animal feeding
stuffs
Enumeration of coagulase positive
staphylococcusISO 6888-2:1999/ Amd1: 2003 Reg2073/2005 ESYD 475-3 16/11/2020
663 3
Food and Animal feeding
stuffs (including carcasses of
cattle, sheep, goats, pigs)
Enumeration of the total aerobic
microorganisms at 30 oC by the pour plate
technique
ISO 4833-1:2013 Reg2073/2005 ESYD 475-3 16/11/2020
664 4Food and Animal feeding
stuffs
Enumeration of b- glucuronidase- positive
Escherichia coli in agarISO 16649-2:2001 Reg2073/2005 ESYD 475-3 16/11/2020
665 5Food and Animal feeding
stuffs (including carcasses of
cattle, sheep, goats, pigs)
Detection and enumeration of
EnterobacteriacaeISO 21528-2: 2004 Reg2073/2005 ESYD 475-3 16/11/2020
666 6Food and Animal feeding
stuffsDetection of Listeria monocytogenes ISO 11290-1: 1996 / Amd 1:2004 Reg2073/2005 ESYD 475-3 16/11/2020
667 7Food and Animal feeding
stuffsEnumeration of Listeria monocytogenes ISO 11290-2: 1998 / Amd 1:2004 Reg2073/2005 ESYD 475-3 16/11/2020
668 8Food and Animal feeding
stuffs
Enumeration of the total aerobic
microorganisms at 30 oC by the surface plating
technique
ISO 4833-2:2013 Reg2073/2005 ESYD 475-3 16/11/2020
669 9Animal faeces &
environmental samples from
the primary production stage
Detection of Salmonella spp ISO 6579: 2002 /Αmd.1: 2007 Reg2160/2003 ESYD 475-3 17/11/2020
670 10 Animal tissues and milk
Detection of antimicrobial agents (quinolones,
cephalosporins, penicillins, macrolides,
tetracyclines, aminoglycosides, sulfonamides)
Method OE 504A-12 based on the method
‘Screening Test for Antibiotic Residues-STAR
Protocol’ of the NRL-Residues
Reg37/2010 ESYD 475-3 17/11/2020
671 11 Pork meat Detection of parasite Τrichinella spp.
Magnetic stirrer method for pooled - sample
digestion (CRL Protocol according to
European Union Commission Regulation No.
1375/2015) ISO 18743:2015
Reg1375/2015 ESYD 475-3 17/11/2020
672 12 Blood serum of ruminantsDetection of antibodies against B. abortus/ B.
melitensis/ B. suis/ B. ovis
Rose Bengal Test (R.B.) according to
Directive 64/432/EEC (and its updates)Dir64/432 ESYD 475-3 17/11/2020
673
Residues of substance group B1 of Annex I of Directive 96/23/EC
Legal basis: Directives 64/432/EEC & 91/68/EEC, Ministerial Decisions 4224/120089/04.11.2015(B'2464) & 3339/117339/20.10.2016(B'3589)
Legal basis: Directive 64/432/ΕEC, Ministerial Desicion 286861/31.12.2003, Circular document no 218465/17.03.2004
Legal basis: Ministerial Decision 3865/128713/22.10.2013
Legal basis: Reguation (EC)2073/2005, Presidential Decree 97/2017 (Α'138)
Legal basis: Regulation (EC) 1069/2011,Regulation (ΕC) 142/2011, Circular document no 226/10890/26.01.2016
Legal basis: Directive 96/23/EC, Presidential Decree 259/1998 (A'191), Approved National Residue Monitoring Plan (2017)
Zoonoses & zoonotic agents (Brucelloses)
Bovine leucosis
Parasites (Trichinella, Echinococcus, Anisakis)
Microbiological criteria for foodstuffs
Animal by-products
Σελίδα 30 από 50
674 NON-ACCREDITED TESTS PERFORMED
675
676 Tested materials/ products Types of test/ Properties to be measured Applied Standards/ Techniques to be used Documentation Comments
677 13 Blood serum of ruminantsDetection of antibodies against B. abortus/ B.
melitensis/ B. suis/ B. ovis Complement Fixation Test (CFT) Dir64/432
678 14 Milk and milk products Count of yeast and moulds at 25 οC ISO 6611:2004 Reg2073/2005
679 15 Milk and udder secretions Mastitis test Whiteside test/ CMT
680 16 Milk and udder secretions Bacterial isolation, AntibiogrammaAntimicrobial Susceptibility–Agar Disk
Diffusion Test according to the valid editions
of CLSI:Μ2, CLSI:M100, Eucast
CLSI M2 M100 EUCAST
681 17 Internal organs Bacterial detection (Gram+, Gram-) Staining, microscopy, culture, isolation
682 18Faecal smears of all animals
and birds
Detection of parasites (eggs, larvae, protozoan
oocysts or cysts etc)Microscopy/Sendimentation/ Flotation
683 19 Bovine blood serum Enzootic leucosis antibodies detection ELISAOIE Terrestrial Manual
2009 Chapter 2.04.10
684 20 Bovine blood serum Enzootic leucosis antibodies detectionAgar gel immunodiffusion (AGID)
OIE Terrestrial Manual
2009 Chapter 2.04.10
685 21 Bovine blood serum Detection of antibodies against Brucella sppELISA (Βrucellosis Antibody Test Kit) for
bovines OIE Terrestrial Manual,2009
Chapter3.2.1
OIE Terrestrial Manual,
2009 Chapter3.2.1
686
Σελίδα 31 από 50
687
688
689
690
691 B.1 DVCTh/DEPARTMENT OF VIRAL, RICKETTSIAL DISEASES AND TRANSMISSIBLE SPONGIFORM ENCEPHALOPATHIES692
693 80, 26th Oktovriou Street, 546 27 Thessaloniki694 tel:+30 210 566066, fax:+30 210 552023695 [email protected]
697 OFFICIAL LABORATORY/LABORATORY DESIGNATED TO CARRY OUT ANALYSES WITHIN THE FIELD OF:698 #699 #700
701 ACCREDITED TESTS PERFORMED702
703 Tested materials/ products Types of test/ Properties to be measured Applied Standards/ Techniques to be used Documentation Certificate number Valid until
704 1
Nervous tissue from the obex
area of brainstem of bovines
and small ruminants (sheep
and goats)
Immuno-enzymatic Elisa test (sandwich
format) for the detection of the abnormal,
resistant to proteinase prion protein in
samples from the Central Nervous System of
infected animals
Rapid test TeSeE (BIORAD) Note:
Purification, concentration and solubilisation
of the PrPres in the samples must precede
Reg.999/2001 &
Reg.270/2002ESYD 844-2 21/11/2020
705 2 Bovine blood serumDetection of antibodies against Enzootic
Bovine Leucosis VirusELISA immunosorbent assay (indirect)
Council Directive
64/432/EEC, Ministerial
Decision
286861/31.12.2003, OIE
Manual of Diagnostic Tests
and Vaccines for Terrestrial
Animals 2012
ESYD 844-2 21/11/2020
706
707 NON-ACCREDITED TESTS PERFORMED708
709 Tested materials/ products Types of test/ Properties to be measured Applied Standards/ Techniques to be used Documentation Comments
710 3 Bovine milkDetection of antibodies against Enzootic
Bovine Leucosis VirusELISA immunosorbent assay (indirect)
Council Directive
64/432/EEC, Ministerial
Decision
286861/31.12.2003, OIE
Manual of Diagnostic Tests
and Vaccines for Terrestrial
Animals 2012
711 4Bovine blood serum & bovine
milk
Detection of antibodies against Enzootic
Bovine Leucosis VirusAgar gel immunodiffusion test
Council Directive
64/432/EEC, Ministerial
Decision
286861/31.12.2003, OIE
Manual of Diagnostic Tests
and Vaccines for Terrestrial
Animals 2012
712 5Bovine blood serum, plasma &
milk
Detection of antibodies against IBR-IPV-IPB
virus ELISA O.I.E. / 4th ed.,2000
713 6Bovine blood serum, plasma &
milkDetection of antibodies against BVD-MD virus ELISA (indirect) O.I.E. / 4th ed.,2000
714 7Bovine blood serum, plasma
or whole blood Detection of BVD-MD virus antigen ELISA O.I.E. / 4th ed.,2000
715 8 Bovine blood serumDetection of antibodies against Bovine
Repiratory Syncytial Virus ELISA
716 9 Sheep & goat blood serumDetection of antibodies against Maedi Visna –
CAE viruses ELISA O.I.E. / 4th ed.,2000
717 10 Blood serum from ruminantsDetection of antibodies against Chlamydia
abortus (chlamydial abortions in sheep & goat) ELISA (indirect) O.I.E. / 4th ed.,2000
718 11Sheep & goat placental
cotyledonsDetection of Chlamydia trachomatis antigen Clearview Chlamydia MF
719 12Blood serum, plasma & milk
from ruminants
Detection of antibodies against Coxiella
burnetii (Q Fever) ELISA O.I.E. / 4th ed.,2000
720
DIRECTORATE OF VETERINARY CENTER OF THESSALONIKI (DVCTh)
Bovine leucosis Legal basis: Directive 64/432/ΕEC, Ministerial Decision 286861/31.12.2003, Circular document no 218465/17.03.2004
Bovine Spongiform Encephalopathy & Small ruminants Transmissible Legal basis: Ministerial Decisions 729/23133/01.03.2017 (B'837) & 730/23143/01.03.2017 (B'952)
Σελίδα 32 από 50
721
722 B.2 DVCTh/DEPARTMENT OF FOOD MICROBIOLOGY, BIOCHEMICAL & PHYSICOCHEMICAL FOOD CONTROL, RESIDUES AND MARINE BIOTOXINS & TOXINS IN OTHER WATERS723
724 80, 26th Oktovriou Street, & 3Α, LIMNOY street, 546 27 Thessaloniki725 τηλ:2310 552928, 2310 566056, 2310 544925726 Fax: 2310 566581, 2310 544927, 2310 566055727 [email protected] ,[email protected] ,[email protected]
729 NATIONAL REFERENCE LABORATORY730 #731
732 OFFICIAL LABORATORY/LABORATORY DESIGNATED TO CARRY OUT ANALYSES WITHIN THE FIELD OF:733 #734 #735 #736 #737 #738
739 ACCREDITED TESTS PERFORMED740
741 Tested materials/ products Types of test/ Properties to be measured Applied Standards/ Techniques to be used Documentation Certificate number Valid until
742 1 Shellfish (bivalve molluscs)
Analysis of lipophilic toxins in shellfish by
mouse bioassay. Qualitative determination of
okadaic acid, dinophysistoxins, pectenotoxins,
yessotoxins and azaspiracids. Detectable
lipophlilic toxins concentration of at least 160
µg okadaic acid equivalents/kg and upwards
Test method (ΔΜΤ.01) for the analysis of
lipophilic toxicity in shellfish by mouse
bioassay, based on the standard method of
Yasumoto et al. (1978) as recommended by
the European Union Reference Laboratory of
Marine Biotoxins (AESAN, Campus
Universitário de Vigo, As Lagoas-Marcosende,
Vigo, Spain)
Reg.2074/2005
Reg.15/2011ESYD 493-3 22/12/2020
743 2 Shellfish (bivalve molluscs)
Analysis of PSP toxicity in shellfish by mouse
bioassay. Range: at least 400 µg STX diHCl
equivalents/kg and upwards
Test method (ΔΜΤ.02) for the analysis of PSP
toxicity in shellfish by mouse bioassay, based
on the AOAC official method 959.08
Reg.2074/2005 ESYD 493-3 22/12/2020
744 3 Shellfish and finfish
Determination of domoic acid concentration
(ASP toxins). Range:≥1.0 mg of domoic acid
equivalents/kg and upwards.
Internal method (ΔΜΤ.03) validated
according to the requirements of the
Decision 2002/657/EC by the European
Union Reference Laboratory of Marine
Biotoxins (AESAN, Campus Universitário de
Vigo, As Lagoas-Marcosende, Vigo, Spain) by
use of High Performance Liquid
Chromatography with Ultraviolet Detection
by Diode Array Detector (HPLC-UV-DAD)
Reg.2074/2005 ESYD 493-3 22/12/2020
745 4 Animal origin foodstuffs Determination of cadmium (Cd) content OE 504 A-1 In-house method using Graphite
Furnace Atomic Absorption Spectroscopy
Reg.333/2007, 836/2011,
582/2016, 1881/2006,
629/2008, 420/2011,
488/2014, 1005/2015
ESYD 492-3 22/12/2020
Viral and bacteriological contamination of bivalve molluscs
Animal by-products
Antimicrobial resistance
Residues of substance group B3c of Annex I of Directive 96/23/EC
Legal basis: Reguation (EC)2073/2005, Presidential Decree 97/2017 (Α'138)
Legal basis: Regulation (EC) 1069/2011,Regulation (ΕC) 142/2011, Circular document no 226/10890/26.01.2016
Legal basis: Desicion 2013/652/ΕU, Joint Ministerial Desicion 1920/88493/22.08.2017 (Β'3225)
Legal basis: Directive 96/23/EC, Presidential Decree 259/1998 (A'191), Approved National Residue Monitoring Plan (2017)
Monitoring of marine biotoxins Legal basis: Regulation (EC) 2074/2005, Ministerial Decision 3865/12873/22.10.2013
Microbiological criteria for foodstuffs
Σελίδα 33 από 50
746 5 Animal origin foodstuffs Determination of lead (Pb) contentOE 504 A-2 In-house method using Graphite
Furnace Atomic Absorption Spectroscopy
Reg.333/2007, 836/2011,
582/2016, 1881/2006,
629/2008, 420/2011,
488/2014, 1005/2015
ESYD 492-3 22/12/2020
747 6 Fish and fishery products Determination of mercury (Hg) content
OE 504 A-4 In-house method using Atomic
Absorption Spectroscopy FI-MH-AAS
technique
Reg.333/2007, 836/2011,
582/2016, 1881/2006,
629/2008, 420/2011,
488/2014, 1005/2015
ESYD 492-3 22/12/2020
748 7 Fish and fishery products Determination of HistamineOE 504 A-3 In-house method based on the
Reference method of Regulation (EC)
2073/2005 by HPLC-DAD
Reg.2073/2005,
1441/2007, 1019/2013 ESYD 492-3 22/12/2020
749 8
Fish and fish products. Milk
and milk products. Meat and
meat products, animal feeding
stuff, eggs and egg products
Detection of Salmonella spp.
(Salmonella typhi and paratyphi - not included)ISO 6579:2017
Reg.882/2004, 1441/2007,
854/2004, 2073/2005ESYD 844-2 21/11/2020
750 9 Molluscan bivalve shellfish Enumeration of beta-glucuronidase positive
Escherichia ColiISO 16649-3:2015 Reg.882/2004, 1441/2007,
854/2004, 2073/2005ESYD 844-2 21/11/2020
751
752 NON-ACCREDITED TESTS PERFORMED753
754 Tested materials/ products Types of test/ Properties to be measured Applied Standards/ Techniques to be used Documentation Comments
755 10. Shellfish Determination of lipophylic biotoxinsUltra High Performance Liquid
Chromatography -Mass Spectrometry (UPLC
MS/MS)
Reg.2074/2005
756
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757
758 B.3 DVCTh/DEPARTMENT OF AVIAN & BEE DISEASES AND AQUATIC ORGANISMS' PATHOLOGY759
760 80, 26th Oktovriou Street, 546 27 Thessaloniki761 tel:+30 210 566051, fax:+30 210 552023762 [email protected]
764 NATIONAL REFERENCE LABORATORY765 # Avian influenza766 #767
768OFFICIAL LABORATORY/LABORATORY DESIGNATED TO CARRY OUT ANALYSES WITHIN THE FIELD OF:
769 #770 #771 #772 #773 #774 #775
776 ACCREDITED TESTS PERFORMED777
778 Tested materials/ products Types of test/ Properties to be measured Applied Standards/ Techniques to be used Documentation Certificate number Valid until
779 1Sera samples from domestic
and wild birdsDetection of Avian Influenza antibodies ELISA immunosorbent assay
Kit IDEXX (Avian Influenza)
for chicken and turkeys. Kit
C-ELISA IDEXX (Avian
Influenza) for domestic and
wild birds
ESYD 844-2 21/11/2020
780 2Sera samples from domestic
and wild birds
Detection of antibodies to different subtypes of
Avian Influenza virusHaemagglutination Inhibition test
OIE Terrestrial Manual
2017 Chapter 2.3.4ESYD 844-2 21/11/2020
781 3Chicks, (live and dead), swabs,
domestic and wild birds
Avian Influenza matrix, H5, H7 and N1 genes
detection Real-Time RT-PCR
Manual VLA (2005/94/ΕC
and 2006/437/ΕC)ESYD 844-2 21/11/2020
782 4Allantoic fluid from chicken
embryonated eggs
Detection and characterization of Avian
Influenza virus
Haemagglutination test for the detection of
haemagglutinating factor and
haemagglutination inhibition test for
characterization of Avian Influenza virus
OIE Terrestrial Manual
2017 Chapter 2.3.4ESYD 844-2 21/11/2020
783 5Live chicks, avian blood and
blood serum samplesDetection of Newcastle Disease antibodies ELISA immunosorbent assay
Kit IDEXX (Newcastle
Disease) for chickenESYD 844-2 21/11/2020
784 6
Animal Faeces and
environmental samples from
the primary production stage
Detection of Salmonella spp. Microbiological assayISO 6579:2002/Amd1:
2007 Annex DESYD 844-2 21/11/2020
785 7Bees, brood and beehive
debris
Detection of Chronic Bee Paralysis Virus
(CBPV) by real time RT-PCR Real-Time RT-PCR
ANSES : SOP Detection and
Quantification of the
chronic bee paralysis virus
(CBPV) by real-time RT-
PCR ANA-1.MOA.1300
ESYD 844-2 21/11/2020
786 8
Bivalve molluscs (mussels,
oysters, clams, cockles,
Bearded horse mussels)
Cytological and Histological detection of
Marteilia sp parasite
Cytological and histological analysis for
bivalve molluscs according to Chapter 2.4.4
of the Manual of Diagnostic Tests for Aquatic
Animals (2016) of the World Organisation
for Animal Health (OIE)
OIE Manual of diagnostic
test for aquatic animals
IFREMER CRL for mollusk
diseases
ESYD 844-2 21/11/2020
787
Bivalve moluscs diseases
Legal basis: Directive 2005/94/EC, Presidential Decree 33/2008 (Α'59), Ministerial Desicion 4411/138539/22.12.2017 (Β'4669)
To be designated soon
Zoonoses & zoonotic agents (Samonella)
Newcastle disease
West Nile fever
Honeybee health
Fish diseases
Diseases of crustaceans
Legal basis: Regulation (EC) 2160/2003, Ministerial Decisions 349699/31.12.2009(B'2599), 133362/23.12.2011 (B' 3172), 1209/30007/13.03.2012(B'930) &
Legal basis: Presidential Decree 97/2017 (Α'138), Ministerial Decision 279719/19.11.2004(Β'1760)
Legal basis: Ministerial Decision 27/3259/14.01.2015(Β'142)
Legal basis: Ministerial Decision 4424/137557/11.11.2013(Β'2923)Legal basis: Presidential Decree 97/2017 (Α'138), Ministerial Desicion 4619/124229/07.12.2012 (Β'3434)
Legal basis: Presidential Decree 97/2017 (Α'138), Ministerial Desicion 4619/124229/07.12.2012 (Β'3434)
Σελίδα 35 από 50
788 NON-ACCREDITED TESTS PERFORMED789
790 Tested materials/ products Types of test/ Properties to be measured Applied Standards/ Techniques to be used Documentation Comments
791 9 Embryonated chicken eggs Isolation of avian influenza virus Inoculation in embryonated chicken eggsOIE Terrestrial Manual
2017 Chapter 2.3.4
792 10 Embryonated chicken eggs Isolation of Newcastle disease virus Inoculation in embryonated chicken eggsOIE Terrestrial Manual
2017 Chapter 2.3.14
793 11Allantoic fluid from chicken
embryonated eggs
Detection and characterization of Newcastle
disease virus
Haemagglutination test for the detection of
haemagglutinating factor and
haemagglutination inhibition test for
characterization of Newcastle disease virus
OIE Terrestrial Manual
2017 Chapter 2.3.14
794 12Sera samples from domestic
and wild birds
Detection of antibodies to Newcastle disease
virusHaemagglutination Inhibition test
OIE Terrestrial Manual
2017 Chapter 2.3.14
795 13Sera samples from domestic
and wild birds
Detection of antibodies to Mycoplasma
gallisepticumELISA immunosorbent assay
Kit IDEXX (Mycoplasma
gallisepticum ) for chickens
and turkeys
796 14 Chicks, (live and dead), swabs Detection of West Nile fever virus genome Real-Time RT-PCRKit LSI - TaqVet
West Nile Virus
797 15Sera samples from domestic
and wild birdsDetection of antibodies to West Nile fever virus ELISA immunosorbent assay
Kit ID-Vet, OIE Terrestrial
Manual, Ch. 2.1.20, West
Nile Fever
798 16 Apis mellifera honey bees Average Nosema sp. spore count per sample Spore counts in a haemacytometer
O.I.E. Terrestrial Manual
(May 2013) Nosemosis of
honey bees (Chapter 2.2.4.)
PD
4424/137557/11.11.2013
799 17Nosema sp. spores in Apis
mellifera honey beesNosema apis & Nosema ceranae differentiation conventional PCR
O.I.E. Terrestrial Manual
(May 2013) Nosemosis of
honey bees (Chapter 2.2.4.)
PD
4424/137557/11.11.2013
800 18 Apis mellifera honey bees Detection of Malpighamoeba mellificae cysts Microscopic examination
O.I.E. Terrestrial Manual
(May 2013) Nosemosis of
honey bees (Chapter 2.2.4.)
PD
4424/137557/11.11.2013
801 19
Apis mellifera honey bees and
brood, broodcomb, hive
debris
Varroa destructor infestationMacroscopic & microscopic examination and
characterization
O.I.E. Terrestrial Manual
(May 2008) Varroosis of
honey bees (Chapter 2.2.7.)
802 20Apis mellifera honey bees,
broodcomb, hive debrisTropilaelaps sp. infestation
Macroscopic & microscopic examination and
characterization
O.I.E. Terrestrial Manual
(May 2017) Infestation of
honey bees with
Tropilaelaps spp (Chapter
2.2.6.), Terrestrial Animal
Health Code, Article 9.5.
803 21 Apis mellifera honey bees Acarapisosis of honey beesMacroscopic & microscopic examination and
characterization
O.I.E. Terrestrial Manual
(May 2008) Acarapisosis of
honey bees (Infestation of
honey bees with Acarapis
woodi) (Chapter 2.2.1.)
804 22Honey bees, larva/pupa,
brood comb
Detection of Paenibacillus larvae (American
Foulbrood, AFB)
Culture methods, microscopic examination of
staining smears
O.I.E. Terrestrial Manual
(May 2016) American
Foolbrood of honey bees
(Infection of honey bees
with Paenibacillus larvae)
(Chapter 2.2.2.), Terrestrial
Animal Health Code, Article
9.2.
Σελίδα 36 από 50
805 23 Honey bees, broodDetection of Melissococcus plutonius (European
Foulbrood, EFB)
Culture methods, microscopic examination of
staining smears
O.I.E. Terrestrial Manual
(May 2016) European
Foolbrood of honey bees
(Infection of honey bees
with Melissococcus
plutonius) (Chapter 2.2.3.),
Terrestrial Animal Health
Code, Article 9.3.
806 24 Honey bees Honeybee virus CBPV conventional PCR
ANSES, PI: Screening for
several honeybee viruses
using PCR (ABPV, IAPV,
KBV, DWV,SBV, BQCV,
CBPV) ANA-I1.MOA.2900
807 25 Brood comb, hive debris Detection of Ascosphaera apis ( Chalkbrood) Macroscopic & microscopic examinationJournal of Apicultural
Research 52(1): (2013),
(Beebook, Coloss)
808 26 Brood comb Detection of Aspergillus flavus (Stonebrood) Macroscopic & microscopic examinationJournal of Apicultural
Research 52(1): (2013),
(Beebook, Coloss)
809 27Apis mellifera honey bees,
brood comb, beehivesSmall Hive Beetle infestation
Macroscopic & stereoscopic examination and
characterization
O.I.E. Terrestrial Manual
(July 2017) Infestation with
Aethina tumida (small hive
beetle) (Chapter
2.2.5.),Terrestrial Animal
Health Code, Article 9.4.
810 28 Hornets and waspsDetection of Vespa velutina (Asian hornet and
others Vespidae sp)
Macroscopic & microscopic examination and
characterization(EURL, method)
811 29 Adults, larvaeDetection of Galleria mellonella (Greater wax
moth)Macroscopic & stereoscopic examination
Journal of Apicultural
Research 52(1): (2013),
(Beebook, Coloss)
812 30 Adults, larvaeDetection of Achroia grisella (Lesser wax
moth)Macroscopic & stereoscopic examination
Journal of Apicultural
Research 52(1): (2013),
(Beebook, Coloss)
813 31 Tissues from bivalve molluscs
Detection of Marteillia spp genome and other
pathogens as well (Vibrio spp, ΟsHV, Bonamia
spp)
PCR / Agarose gel electrophoresis and
obsevation under a bank of UV lamps
OIE Manual of diagnostic
test for aquatic animals
IFREMER CRL for mollusk
diseases
814 32Tissues from fish, bivalve
moluscs and crustaceans Detection of Vibrio spp and Aeromonas spp
Innoculation in TSA marine matrix & blood
agar, Characterization of colonies with api
20Ε system
OIE Manual of diagnostic
test for aquatic animals
IFREMER CRL for mollusk
diseases
815 33Tissues from fish and
crustaceansParasites infestation
Microscopic examination of methylene blue
stained fresh specimens
OIE Manual of diagnostic
test for aquatic animals
IFREMER CRL for mollusk
diseases816
Σελίδα 37 από 50
817
818 B.4 DVCTh/DEPARTMENT OF MICROBIOLOGY, INFECTIOUS DISEASES & BRUCELLOSIS819
820 80, 26th Oktovriou Street, 546 27 Thessaloniki821 tel:+30 210 566038, fax:+30 210 552023822 [email protected]
824 OFFICIAL LABORATORY/LABORATORY DESIGNATED TO CARRY OUT ANALYSES WITHIN THE FIELD OF:825 #826
827 ACCREDITED TESTS PERFORMED828
829 Tested materials/ products Types of test/ Properties to be measured Applied Standards/ Techniques to be used Documentation Certificate number Valid until
830 1Blood serum samples from
ruminants
ELISA immunosorbent assay for the detection
of paratuberculosis disease antibodies
Kit Paratuberculosis LSIVet screening for
ruminants
OIE Terrestrial Manual
2017 Chapter 2.1.15.ESYD 844-2 21/11/2020
831 2 Blood serum samples of
domestic or wild ruminants,
Detection of antibodies against Βrucella
abortus, B. melitensis, B. suis
Rose Bengal Test (R.B.) according to the
Commission Regulation 535/2002/EC Reg.535/2002 ESYD 844-2 21/11/2020
832
833 NON-ACCREDITED TESTS PERFORMED834
835 Tested materials/ products Types of test/ Properties to be measured Applied Standards/ Techniques to be used Documentation Comments
836 3 Blood serum from ruminantsDetection of antibodies against Brucella
abortus, B. melitensisComplement Fixation Test
Reg.535/2002, MD
4224/120089/OGG2464Β/
2015, MD
3339/117339/OGG3589Β/
2016 OIE Terrestrial
Manual,2016 Chapter2.1.4
837 4 Bovine blood serumDetection of antibodies against Brucella
abortus, B. melitensis
ELISA / Kit IDEXX (Βrucellosis Antibody Test
Kit) for bovines /Kit PrioCHECK for bovines
Reg.535/2002, MD
4224/120089/OGG2464Β/
2015 OIE Terrestrial
Manual,2016 Chapter2.1.4
838 5 Bovine milk serumDetection of antibodies against Brucella
abortus
ELISA / Kit IDEXX (Βrucellosis Antibody Test
Kit) for bovines / Kit PrioCHECK for bovines
Reg.535/2002 MD
4224/120089/OGG2464Β/
2015 OIE Terrestrial
Manual,2016 Chapter2.1.4
839 6 Spleen sections/blood Detection of Bacillus anthracis Culture, microscopyOIE Terrestrial
Manual,2017 Chapter 2.1.1
840 7Sections of small/large
intestine & mesenteric lymph
nodes - Faeces
Detection of Mycobacterium paratuberculosis Microscopy OIE Terrestrial
Manual,2017 Chapter
2.1.15
Zoonoses & zoonotic agents (Brucelloses) Legal basis: Directives 64/432/EEC & 91/68/EEC, Ministerial Decisions 4224/120089/04.11.2015(B'2464) & 3339/117339/20.10.2016(B'3589)
Σελίδα 38 από 50
841 8Sections of myocardium,
muscles, bones, internal
organs
Detection of Clostridium chauvoei, Clostridium
perfringensCulture, microscopy, biochemical testing
Clinical Veterinary
Microbiology 2nd ed Mosby
2013
842 9 Brain and liver sections Detection of Listeria monocytogenesCulture, microscopy, biochemical testing (ISO
11290-1:2017)
OIE Terrestrial
Manual,2017 Chapter 2.9.6
843 10Intestines, faeces, mesenteric
lymph nodes from pigsDetection of Salmonella spp
Culture, microscopy, biochemical testing (ΙSO
6579-1:2017)
OIE Terrestrial
Manual,2017 Chapter 2.9.8
844 11Lung tissue, synovial fluid,
milk secretionDetection of Mycoplasma spp Culture, stereoscopy
Clinical Veterinary
Microbiology 2nd ed Mosby
2013
845 12 Sheep and goat blood serumDetection of antibodies against Mycoplasma
agalactiae
ELISA (Pourquier ELISA Mycoplasma
agalactiae verification ΙDEXX)
OIE Terrestrial Manual
2017, Chapter 2.7.4
846 13 Lung tissueDetection of Pasteurella
multocida/Mannheimia haemolyticaCulture, microscopy, biochemical testing
Clinical Veterinary
Microbiology 2nd ed Mosby
2013
847 14Aborted fetuses, preputial
washing
Detection of Campylobacter spp &
Tritrichomonas spp
Culture, microscopy, biochemical testing (ISO
10272-1:2017)
OIE Terrestrial
Manual,2017 Chapter 2.4.4
& 2.4.16
848 15 Internal organs
Detection of Escherichia coli , Corynebacterium
spp, Staphylococcus aureus,Staphylococcus spp,
Streptococcus spp
Culture, microscopy, biochemical testing
Clinical Veterinary
Microbiology 2nd ed Mosby
2013
849 16Internal organs, joints, lungs
from pigs, rabbits and
ruminants
Detection of Haemophilus spp, Βordetella spp,
Erysipelothrix sppCulture, microscopy, biochemical testing
Clinical Veterinary
Microbiology 2nd ed Mosby
2013
850 17 Internal organs Detection of Mycobacterium spp MicroscopyClinical Veterinary
Microbiology 2nd ed Mosby
2013
851 18 Cultures Antimicrobial resistance Antimicrobial Susceptibility–Agar Disk
Diffusion Test according to the valid editions
of CLSI:Μ2, CLSI:M100, Eucast
Clinical Veterinary
Microbiology 2nd ed Mosby
2013
852 19 Milk and udder secretionsDetection of Escherichia coli, Klebsiella spp,
Enterobacter spp, Proteus spp and other
enterobacteriaceae
Culture, microscopy, biochemical testingClinical Veterinary
Microbiology 2nd ed Mosby
2013
853 20 Milk and udder secretions
Detection of Staphylococcus aureus, coagulase
negative staphylococci Streptococcus
agalactiae , Streptococcus uberis,
Streptococcus dysgalactiae , Streptococcus spp,
Enterococcus spp
Culture, microscopy, biochemical testing
Clinical Veterinary
Microbiology 2nd ed Mosby
2013
854 21 Milk and udder secretionsDetection of Pasteurella spp, Pseudomonas
spp, Corynebacterium spp, Trueperella
pyogenes
Culture, microscopy, biochemical testingClinical Veterinary
Microbiology 2nd ed Mosby
2013
855 22Milk and udder secretions,
internal organsDetection of yeasts - moulds Culture, microscopy
Clinical Veterinary
Microbiology 2nd ed Mosby
2013856
Σελίδα 39 από 50
857
858 Β.5 DVCTh/DEPARTMENT: VETERINARY LABORATORY OF IOANNINA859
860 Chr. Katsari 2, PC 454 44, Ioannina861 tel: +30 26510 27396, fax: +30 26510 33541862 [email protected] 863
864 OFFICIAL LABORATORY/LABORATORY DESIGNATED TO CARRY OUT ANALYSES WITHIN THE FIELD OF:865 #
866 #
867 #868 #
869 #
870 #
871 #
872 #
873 #
874
875 ACCREDITED TESTS PERFORMED876
877 Tested materials/ products Types of test/ Properties to be measured Applied Standards/ Techniques to be used Documentation Certificate number Valid until
878 1 Food and animal feeding stuffDetection of Salmonella spp ( except S. typhi , S.
paratyphi)ISO 6579-1:2017
Reg2073/2005,
Reg1441/2007,
Reg365/2010,
Reg1086/2011,
Reg209/2013
ESYD488-3 16/12/2020
879 2 Food and animal feeding stuff Detection of Listeria monocytogenes ISO 11290-1:2017
Reg2073/2005,
Reg1441/2007,
Reg365/2010,
Reg1086/2011,
Reg209/2013
ESYD488-3 16/12/2020
880 3 Food and animal feeding stuff Enumeration of Listeria monocytogenes ISO 11290-2:2017
Reg2073/2005,
Reg1441/2007,
Reg365/2010,
Reg1086/2011,
Reg209/2013
ESYD488-3 16/12/2020
881 4 Food and animal feeding stuffEnumeration of coagulase positive
staphylococciISO 6888-2:1999
Reg2073/2005,
Reg1441/2007,
Reg365/2010,
Reg1086/2011,
Reg209/2013
ESYD488-3 16/12/2020
882 5 Food and animal feeding stuffEnumeration of β-glucoronidase positive
Escherichia coli ISO 16649-2:2001
Reg2073/2005,
Reg1441/2007,
Reg365/2010,
Reg1086/2011,
Reg209/2013
ESYD488-3 16/12/2020
883 6 Food and animal feeding stuffEnumeration of the total aerobic
microorganisms at 30 oCISO 4833-1 :2013
Reg2073/2005,
Reg1441/2007,
Reg365/2010,
Reg1086/2011,
Reg209/2013
ESYD488-3 16/12/2020
884 7 Food and animal feeding stuffDetection and enumeration of
enterobacteriaceaeISO 21528-2:2017
Reg2073/2005,
Reg1441/2007,
Reg365/2010,
Reg1086/2011,
Reg209/2013
ESYD488-3 16/12/2020
885 8
Animal faeces and samples
from the primary production
stage
Detection of Salmonella spp ( except S. typhi , S.
paratyphi)ISO 6579-1:2017
Reg2073/2005,
Reg1441/2007,
Reg365/2010,
Reg1086/2011,
Reg209/2013
ESYD488-3 16/12/2020
886 9 Animal and poultry tissues
Detection of antimicrobial agents (quinolones,
cephalosporins, penicillins, macrolides,
tetracyclines, aminoglycosides, sulfonamides)
Method OE504A-28 based on the method
“Screening Test for Antibiotic Residues
–STAR Protocol” of the NRL-Residues
Reg657/2002,
Reg37/2010, GUIDELINES
FOR THE VALIDATION OF
SCREENING METHODS (20-
1-2010)
ESYD488-3 16/12/2020
887 10
Digestion residues from
biogas plants – compost from
composting plants
Detection of Salmonella spp ( except S. typhi , S.
paratyphi)
Based on ISO 6579-1:2017 for the purpose of
Commission Regulation (EC) No 1069/2009
and implementing Regulation (EU) No
142/2011
Reg1069/2011,
Reg142/2011ESYD488-3 16/12/2020
Microbiological criteria for foodstuffs
Milk and milk products (criteria for raw milk)
Legal basis: Directives 64/432/EEC & 91/68/EEC, Ministerial Decisions 4224/120089/04.11.2015(B'2464) & 3339/117339/20.10.2016(B'3589)
Legal basis: Regulation (EC) 2160/2003, Ministerial Decisions 349699/31.12.2009(B'2599), 133362/23.12.2011 (B' 3172), 1209/30007/13.03.2012(B'930) &
1751/51822/24.04.2013(B'1141)Legal basis: Directive 64/432/ΕEC, Ministerial Desicion 286861/31.12.2003, Circular document no 218465/17.03.2004Legal basis: Ministerial Decisions 729/23133/01.03.2017 (B'837) & 730/23143/01.03.2017 (B'952)
Legal basis: Regulation (EC) 1069/2011,Regulation (ΕC) 142/2011, Circular document no 226/10890/26.01.2016
Legal basis: Desicion 2013/652/ΕU, Joint Ministerial Desicion 1920/88493/22.08.2017 (Β'3225)
Legal basis: Directive 96/23/EC, Presidential Decree 259/1998 (A'191), Approved National Residue Monitoring Plan (2017)
Legal basis: Reguation (EC)2073/2005, Presidential Decree 97/2017 (Α'138)
Legal basis: Ministerial Desicion 3908/139386/14.12.2015 (Β'2833)
Zoonoses & zoonotic agents (Brucelloses)
Zoonoses & zoonotic agents (Samonella)
Bovine leucosis
Bovine Spongiform Encephalopathy & Small ruminants Transmissible
Animal by-products
Antimicrobial resistance
Residues of substance group B1 of Annex I of Directive 96/23/EC
Σελίδα 40 από 50
888 11
Digestion residues from
biogas plants – compost from
composting plants
Enumeration of β-glucoronidase positive
Escherichia coli
Based on ISO 16649-2:2001 for the purpose
of Commission Regulation (EC) No
1069/2009 and implementing Regulation
(EU) No 142/2011
Reg1069/2011,
Reg142/2011ESYD488-3 16/12/2020
889 12Nervous tissue (obex) of
bovine, ovine and caprine
originDetection of the protein PrPRes Immuno-enzymatic technique (TeSeE BIO-
RAD rapid test) according to the Commission Reg999/2001 ESYD488-3 16/12/2020
890 13
Blood serum samples of
domestic or wild ruminants,
equine, swine, camelids,
carnivores and human origin
Detection of antibodies against Βrucella
abortus/ melitensis/ suis
Rose Bengal Test (R.B.) according to the
Commission Regulation 535/2002/EC
amending Annex C of the Directive
64/432/EEC and the Decision 2000/330/EC
Dir64/432/EEC,
Reg535/2002ESYD488-3 16/12/2020
891 14
Blood serum samples of
domestic or wild ruminants,
equine, swine, camelids,
carnivores and human origin
Detection of antibodies against Βrucella
abortus/ melitensis/ suis
Complement Fixation Test (C.F.T.) according
to the Commission Regulation 535/2002/EC
amending Annex C of the Directive
64/432/EEC and the Decision 2000/330/EC
Dir64/432/ΕEC,
Reg535/2002ESYD488-3 16/12/2020
892 15Blood serum samples of
bovine
Detection of antibodies against Bovine Leucosis
Virus
ELISA Test according to the Commission
Directive 64/432/EC, the Decision
2009/976/ΕΕ and the OIE Manual of
Diagnostic Tests and Vaccines for Terrestrial
Animals 2012
Dir64/432/ΕEC,
Dec2009/976/ΕUESYD488-3 16/12/2020
893 16Milk samples of domestic or
wild ruminants
Detection of antibodies against Βrucella
abortus/ melitensis/ suis
ELISA Test according to the Commission
Regulation 535/2002/EC amending Annex C
of the Directive 64/432/EEC and the
Decision 2000/330/EC
Dir64/432/ΕEC,
Reg535/2002ESYD488-3 16/12/2020
894
895 NON-ACCREDITED TESTS PERFORMED
896
897 Tested materials/ products Types of test/ Properties to be measured Applied Standards/ Techniques to be used Documentation Comments
898 17 Bovine blood serum sampleDetection of antibodies against Bovine Leucosis
VirusAgar gel immunodiffusion test
Dir64/432/ΕΟΚ,
Dec2009/976/ΕU, OIE
Manual of Diagnostic Tests
and Vaccines for Terrestrial
Animals 2012
899 18 Avian caeca Detection of C. jejuni ISO 10272-1 modifiedDec2013/653,
JMD1571/72152/2014
900 19 caecal samplesDetection of ESBL-, AmpC- and carbapenemase-
producing E. coli EURL-AR DTU November 2017 5th edition
Desicion 2013/652/ΕU,
Joint Ministerial Desicion
1920/88493/22.08.2017
(Β'3225)
901 20 fresh meatDetection of ESBL-, AmpC- and carbapenemase-
producing E. coli EURL-AR DTU October 2015 3rd edition
Desicion 2013/652/ΕU,
Joint Ministerial Desicion
1920/88493/22.08.2017
(Β'3225)
902 21 Bovine milk sampleDetection of antibodies against Bovine Leucosis
VirusELISA Test
Dir64/432/ΕΟΚ,
Dec2009/976/ΕU, OIE
Manual of Diagnostic Tests
and Vaccines for Terrestrial
Animals 2012903
Σελίδα 41 από 50
904
905 Β.6 DVCTh/DEPARTMENT: VETERINARY LABORATORY OF KAVALA906
907 Stavros Amygdaleona, PC: 640 12, Kavala908 tel: +30 2510 391865, fax: +30 2510 392301 909 [email protected] 910
911 NATIONAL REFERENCE LABORATORY912 #913
914 OFFICIAL LABORATORY/LABORATORY DESIGNATED TO CARRY OUT ANALYSES WITHIN THE FIELD OF:915 #
916 #
917 #
918 #
919 #
920
921 ACCREDITED TESTS PERFORMED922
923 Tested materials/ products Types of test/ Properties to be measured Applied Standards/ Techniques to be used Documentation Certificate number Valid until
924 1 Bivalve molluscsEnumeration of β-glucuronidase positive E.
Coli
ISO/TS 16649-3:2015 Most probable
number, using 5-bromo-4-chloro-3-indolyl-β-
D-glucuronide
Reg2073/2005,
Reg1441/2007ESYD529-3 29/3/2021
925 2Food except small solid
foodstuff
Detection of Salmonella spp. Horizontal
method for the detection of non-typhi, non-
paratyphι Salmonella spp.
ISO 6579:1:2017Reg2073/2005,
Reg1441/2007ESYD529-3 29/3/2021
926 3Food and Animal Feeding
stuffsDetection of Listeria monocytogenes ISO 11290-1:2017
Reg2073/2005,
Reg1441/2007ESYD529-3 29/3/2021
927 4 Animal tissues and milk
Detection of antimicrobial agents (quinolones,
cephalosporins, penicillins, macrolides,
tetracyclines, aminoglycosides, sulfonamides)
Method MD-07 based on “Screening Test for
Antibiotic Residues (STAR Protocol” of the
LVM/RMV/BIO/PTC/ 15.AN/17-10-2006
Protocol of Community Reference
Laboratory of AFFSA-FOUGERES 6th Edition)
Reg657/2002,
Reg37/2010, GUIDELINES
FOR THE VALIDATION OF
SCREENING METHODS (20-
1-2010
ESYD529-3 29/3/2021
928 5
Blood serum samples of
domestic or wild ruminants,
equine, swine, camelids,
carnivores and human origin
Detection of antibodies against Βrucella
abortus, B. melitensis, B. suis
Rose Bengal Test (R.B.) according to
the Commission Regulation 535/2002/EC
amending Annex C of the Directive
64/432/EEC and the Decision 2000/330/EC
Dir64/432/EEC ESYD529-3 29/3/2021
929
930 NON-ACCREDITED TESTS PERFORMED
931
932 Tested materials/ products Types of test/ Properties to be measured Applied Standards/ Techniques to be used Documentation Comments
933 6
Blood serum samples of
domestic or wild ruminants,
equine, swine, camelids,
carnivores and human origin
Detection of antibodies against Βrucella
abortus, B. melitensis, B. suis
Complement Fixation Test (C.F.T.) according
to the Commission Regulation 535/2002/EC
amending Annex C of the Directive
64/432/EEC and the Decision 2000/330/EC
Dir64/432/EEC
934 7 Bovine milk sample Detection of antibodies against Βrucella
abortus, B. melitensis, B. suis
ELISA Test according to the Commission
Regulation 535/2002/EC amending Annex C
of the Directive 64/432/EEC and the
Decision 2000/330/EC
Dir64/432/EEC
935 8 Bovine blood serumDetection of antibodies against Βrucella
abortus, B. melitensis, B. suis
ELISA Test according to the Commission
Regulation 535/2002/EC amending Annex C
of the Directive 64/432/EEC and the
Dir64/432/EEC
936 9 Bovine milk sample Detection of antibodies against Bovine Leucosis
VirusELISA Test
Dir64/432/ΕEC,
Dec2009/976/ΕΕ
937 10Blood serum samples of
bovine
Detection of antibodies against Bovine Leucosis
VirusELISA
Dir64/432/ΕEC,
Dec2009/976/ΕU
938 11Apis mellifera honey bees and
brood
Detection of Varroa destructor (Varroosis)
mites and their counting
Macroscopic & microscopic examination and
identification / OIE Terrestrial Manual 2008
2.2.7
OIE Terrestrial Animal
Health Code , Article 9.6
Residues of substance group B1 of Annex I of Directive 96/23/EC Legal basis: Directive 96/23/EC, Presidential Decree 259/1998 (A'191), Approved National Residue Monitoring Plan (2017)
Honeybee health Legal basis: Ministerial Decision 4424/137557/11.11.2013(Β'2923)
Zoonoses & zoonotic agents (Brucelloses) Legal basis: Directives 64/432/EEC & 91/68/EEC, Ministerial Decisions 4224/120089/04.11.2015(B'2464) & 3339/117339/20.10.2016(B'3589)
Bovine leucosis Legal basis: Directive 64/432/ΕEC, Ministerial Desicion 286861/31.12.2003, Circular document no 218465/17.03.2004
Microbiological criteria for foodstuffs Legal basis: Reguation (EC)2073/2005, Presidential Decree 97/2017 (Α'138)
Viral and bacteriological contamination of bivalve molluscs
Σελίδα 42 από 50
939 12 Apis mellifera honey beesDetection of Tropilaelaps spp. (tropipelalps
infestination of bees)
Macroscopic & microscopic examination and
identification/ OIE Terrestrial Manual 2017
2.2.6
OIE Terrestrial Animal
Health Code, Article 9.5.
940 13 Forager bees Apis mellifera
detection of Nosema spp. (spores) (qualitative
determination) and their count
(quantification)
Microscopic examination, spore count on
hemocytometer and determination of mean
spore number per bee / OIE Terrestrial
Manual 2013 2.2.4
MD
4424/137557/11.11.2013
941 14 Apis mellifera honey bees Detection of Malpighamoeba mellificaeMicroscopic examination of malpighian
tubules/OIE Terrestrial Manual 2013 2.2.4
942 15 Honey bees, broodDetection of Melissococcus plutonius (European
Foul Brood, EFB)
Culture-microscopy / OIE Terrestrial Manual
2016 Chapter 2.2.3
OIE Terrestrial Animal
Health Code , Article 9.3.
943 16 Honey bees, broodDetection of Paenibacillus larvae (American
Foul Brood, AFB)
Culture-microscopy / OIE Terrestrial Manual
2016 Chapter 2.2.2
OIE Terrestrial Animal
Health Code , Article 9.2.
944 17 Brood Detection of Ascosphaera apis Macroscopic observation & microscopy
(Beebook Vollume II, coloss)
945 18 Brood Detection of Aspergillus flavusMacroscopic observation & microscopy
(Beebook Vollume II, coloss)
946 19 Honey bees, brood, beehives Small Hive Beetle infestationMacroscopic observation & stereoscopy - OIE
Terrestrial Manual 2017 2.2.5
OIE Terrestrial Animal
Health Code , Article 9.4.
947 20 Adult waspsDetection of Vespa velutina and other Vespidae
sp.
Macroscopic observation & stereoscopy
(EURL, method)
948 21 Adults, larvaeDetection of Galleria mellonella and Achroia
grisella ( Greater and Lesser max moths )
Macroscopic observation & stereoscopy
(BeeBook Vollume II, Coloss)
949 22 Bees of Apis melliferaDetection of Acarapis woodi mites
(Acarapisosis)
Mιcroscopic observation & identification OIE
Terrestrial Manual 2008 2.2.1.
OIE Terrestrial Animal
Health Code, Article 9.1.
950 23Food and Animal Feeding
stuffsEnumeration of Listeria monocytogenes ISO 11290-2:2017 Reg 2073/2005
951 24Food and Animal Feeding
stuffs
Enumeration of coagulase positive
staphylococciISO 6888-2:1999 Reg 2073/2005
952 25Food and Animal Feeding
stuffs
Enumeration of β-glucoronidase positive
Escherichia coliISO 16649-2:2001 Reg 2073/2005
953 26Food and Animal Feeding
stuffs
Detection and Enumeration of
EnterobacteriaceaeISO 21528-1:2017 & ISO 21528-2:2017 Reg 2073/2005
954 27 Food and Animal Feeding
stuffs
Enumeration of the total aerobic
microorganisms at 30 oCISO 4833-1:2013 Reg 2073/2005
955 28 Dead animals and tissuesNecrotomic, parasitic, microbiological
examinations
Necropsy and sampling for further
microscopic or microbiological examination
956
Σελίδα 43 από 50
957
958 Β.7 DVCTh/DEPARTMENT: VETERINARY LABORATORY OF SERRES959
960 Terma Omonoias, PC 621 10, Serres 961 tel: +30 23210 35411, fax: +30 23210 35410 962 [email protected] 963
964NATIONAL REFERENCE LABORATORY
965 #
966
967 ACCREDITED TESTS PERFORMED968
969 Tested materials/ products Types of test/ Properties to be measured Applied Standards/ Techniques to be used Documentation Certificate number Valid until
970 1 Bovine urine Determination of anabolic steroids: 17α-
trenbolone, methyltestosterone
Internal method OMT.001 by liquid
chromatography-tandem mass spectrometry.
(LC-MS/MS) based on the method of the Eur.
Community Refence lab (RIVM, Bilthoven,
The Netherlands)
Dec657/2002 ESYD 482 19/11/2020
971 2 Bovine urine
Determination of anabolic steroids:
diethylstilbestrol, dienestrol, hexestrol, β-
estradiol, ethynylestradiol, α-zearalanol, β-
zearalanol, stanozolol, 16β-hydroxystanozolol,
α-nortestosterone
Internal method OMT.003 by liquid
chromatography-tandem mass spectrometry.
(LC-MS/MS) based on the method of the Eur.
Community Refence lab (RIVM, Bilthoven,
The Netherlands)
Dec657/2002 ESYD 482 19/11/2020
972 3 Βovine muscle tissueDetermination of anabolic steroids: 17β-
trenbolone, methyltestosterone
Internal method OMT 002 by liquid
chromatography-tandem mass spectrometry.
(LC-MS/MS) based on the method of the Eur.
Community Refence lab (RIVM, Bilthoven,
The Netherlands)
Dec657/2002 ESYD 482 19/11/2020
973 4 FatDetermination of organochlorine pesticides:
cis-chlordane, trans-chlordane, aldrin, dieldrin
Internal method OMT 004 by gas
chromatography-tandem mass spectrometry.
(GC-MS/MS) based on the EPA methods of
pesticides
Dec657/2002 ESYD 482 19/11/2020
974
975 NON-ACCREDITED TESTS PERFORMED
976
977 Tested materials/ products Types of test/ Properties to be measured Applied Standards/ Techniques to be used Documentation Comments
978 5Caprine,Ovine and porcine
urine
Determination of 17α-trenbolone,
methyltestosterone
Liquid chromatography-tandem mass
spectrometry (LC-MS/MS)Dec657/2002 Validated
979 6Caprine,Ovine and porcine
urine
Determination of hormones
(stilbenes)diethylstilbestrol, dienestrol,
hexestrol
Liquid chromatography-tandem mass
spectrometry (LC-MS/MS)Dec657/2002 Validated
980 7 Bovine urineDetermination of α-zearalenol, β-zearalenol,
zearalenone, α-boldenone, β-boldenone, β-
testosterone
Liquid chromatography-tandem mass
spectrometry (LC-MS/MS)Dec657/2002 Validated
981 8Muscle tissue of
caprines,sheep,fisheries,
poultry, rabbits, game animals
Determination of 17β-trenbolone,
methyltestosterone
Liquid chromatography-tandem mass
spectrometry (LC-MS/MS)Dec657/2002 Validated
Residues of substance groups A1, Α3, Α4, Α6 (dapsone), Β2f (carbadox,
olaquindox), Β3a of Annex I of Directive 96/23/ECLegal basis: Desicion 98/563/ΕC
Σελίδα 44 από 50
982 9
Muscle tissue of fisheries,
bovines, ovines, caprines,
poultry, rabbits, wild and
game animals
Determination of diethylstilbestrolLiquid chromatography-tandem mass
spectrometry (LC-MS/MS)Dec657/2002 Validated
983 10Blood samples from bovines,
ovines, caprines, avians,
porcines
Determination of lactones: α-zearalanol, β-
zearalanol
Liquid chromatography-tandem mass
spectrometry (LC-MS/MS)Dec657/2002 Validated
984 11
Muscle tissue of fisheries,
bovines, ovines, caprines,
poultry, rabbits, wild and
game animals
Determination of lactones:zearalenone, α-
zearalanol (zeranol), α- zearalenol, β-
zearalanol (taleranol), β- zearalenol
Liquid chromatography-tandem mass
spectrometry (LC-MS/MS)Dec657/2002 Validated
985 12 Ovine, caprine and porcine
urine
Determination of hormones:β-estradiol,
ethynylestradiol, stanozolol, 16β-OH-
hydroxystanozolol, α-nortestosterone, α-
boldenone, β-boldenone, β- testosterone
Liquid chromatography-tandem mass
spectrometry (LC-MS/MS)Dec657/2002 Validated
986 13
Liver and muscle tissue
(fisheries, bovines, ovines,
caprines, poultry, rabbits, wild
and game animals)
Determination of corticosteroids:
dexamethasone, flumethasone, triamcinolone
acetonide
Liquid chromatography-tandem mass
spectrometry (LC-MS/MS)Dec657/2002 Validated
987 14 Fat
Determination of gestagens:
medroxyprogesterone acetate, megestrol
acetate, melengestrol acetate, chlormadinone
acetate
Liquid chromatography-tandem mass
spectrometry (LC-MS/MS)Dec657/2002 Validated
988 15Blood samples from bovines,
ovines, caprines, avians,
porcines
Determination of ethynylestradiolLiquid chromatography-tandem mass
spectrometry (LC-MS/MS)Dec657/2002 Validated
989 16
Muscle tissue of fisheries,
bovines, ovines, caprines,
poultry, rabbits, wild and
game animals
Determination of ethynylestradiolLiquid chromatography-tandem mass
spectrometry (LC-MS/MS)Dec657/2002 Validated
990 17Blood samples from bovines,
ovines, caprines, avians,
porcines
Determination of methyltestosterone, α-
trenbolone, β-trenbolone, α-boldenone, β-
boldenone, α-nortestosterone, β-
nortestosterone
Liquid chromatography-tandem mass
spectrometry (LC-MS/MS)Dec657/2002 Validated
991 18
Muscle tissue of fisheries,
bovines, ovines, caprines,
poultry, rabbits, wild and
game animals
Determination of α-boldenone, β-boldenone, α-
nortestosterone, β-nortestosterone
Liquid chromatography-tandem mass
spectrometry (LC-MS/MS)Dec657/2002 Validated
992 19Bovine, ovine and porcine
urine
Determination of lactones:zearalenone, α-
zearalanol (zeranol), α- zearalenol, β-
zearalanol (taleranol), β- zearalenol
Liquid chromatography-tandem mass
spectrometry (LC-MS/MS)Dec657/2002 Validated
993 20Blood samples from bovines,
ovines, caprines, avians,
porcines
Determination of α-estradiol, β-estradiolLiquid chromatography-tandem mass
spectrometry (LC-MS/MS)Dec657/2002 Validated
994 21
Muscle tissue of fisheries,
bovines, ovines, caprines,
poultry, rabbits, wild and
game animals
Determination of β-estradiolLiquid chromatography-tandem mass
spectrometry (LC-MS/MS)Dec657/2002 Validated
995 22 FatDetermination of HCB, Heptachlor, Endosulfan,
Endrin, p,p`DDE, p,p`DDD- o,p`DDT
Gas chromatography-tandem mass
spectrometry (GC-MS/MS)Dec657/2002 Validated
996 23 FatDetermination of PCB_28, PCB_52, PCB_101,
PCB_118, PCB_180
Gas chromatography-tandem mass
spectrometry (GC-MS/MS)Dec657/2002 Validated
997 24
Muscle tissue of bovines,
ovines, caprines, poultry,
rabbits, wild and game
animals
Determination of carbadox and metabolites
CBX carbadox, QCA quinoxaline-2-carboxylic
acid, mQCA methyl-3-quinoxaline-2-carboxylic
acid
Liquid chromatography-tandem mass
spectrometry (LC-MS/MS)Dec657/2002 Validated
998 25
Muscle tissue of bovines,
ovines, caprines, poultry,
rabbits, wild and game
animals
Determination of dapsoneLiquid chromatography-tandem mass
spectrometry (LC-MS/MS)Dec657/2002 Validated
999 26Milk from bovines, ovines,
caprinesDetermination of dapsone
Liquid chromatography-tandem mass
spectrometry (LC-MS/MS)Dec657/2002 Validated
1000 27
Muscle tissue of fisheries,
bovines, ovines, caprines,
poultry, rabbits, wild and
game animals
Determination of hexestrol, dienestrolLiquid chromatography-tandem mass
spectrometry (LC-MS/MS)
Σελίδα 45 από 50
1001 28 Milk
Determination of α-HCH, HCB, Lindane,
Heptachlor, Heptachlor epoxide, aldrin, α-
Endosulfan, β-Endosulfan, cis-Chlordane, trans-
Chlordane, p,p’-DDE, o,p’-DDT, p,p’-DDD, p,p’-
DDT
Gas chromatography-tandem mass
spectrometry (GC-MS/MS)SANTE/11813/2017 Validated
1002 29 Live bivalve molluscs (mussels) Determination of polychlorionated biphenylsGas chromatography-tandem mass
spectrometry (GC-MS/MS)SANTE/11813/2017 Validated
1003 30 FatDetermination of: α-HCH, β-HCH, Lindane,
Heptachlor epoxide, β-Endosulfan, p,p’-DDT
Gas chromatography-tandem mass
spectrometry (GC-MS/MS)
1004 31 FatDetermination of Polychlorinated biphenyls:
PCB138, PCB153
Gas chromatography-tandem mass
spectrometry (GC-MS/MS)
1005 32 Honey,eggs, fisheriesDetermination of Polychlorinated biphenyls:
PCB28, PCB52, PCB101, PCB118, PCB138,
PCB153, PCB180
Gas chromatography-tandem mass
spectrometry (GC-MS/MS)
1006 33 Honey,eggs, fisheries
Determination of: α-HCH, HCB, Lindane, β-HCH,
Heptachlor, Heptachlor epoxide, Aldrin,
Dieldrin, Endrin, α-Endosulfan, β-Endosulfan,
cis-Chlordane, trans-Chlordane, p,p’-DDE, o,p’-
DDT, p,p’-DDD, p,p’-DDT
Gas chromatography-tandem mass
spectrometry (GC-MS/MS)
1007 34 MilkDetermination of: PCB28, PCB52, PCB101,
PCB118, PCB138, PCB153, PCB180
Gas chromatography-tandem mass
spectrometry (GC-MS/MS)
1008 35 Blood smears of livestockDetection of blood parasites Babesia spp.
Αnaplasma spp.
Microscopic examination of blood stained
with Giemsa-May Grunwald stainO.I.E. Terrestrial Manual
1009 36Faecal smears of livestock,
poultry, game birds and
companion animals
Detection of Cryptosporidium spp oocysts Acid-fast Ziehl-Neelsen stain
O.I.E. Terrestrial Manual
(May 2016)
Cryptosporidiosis (Chapter
2.9.4.)
1010 37Sections of small/large
intestine & mesenteric lymph
nodes - Faeces
Detection of Mycobacterium paratuberculosis Ziehl-Neelsen stain, microscopy OIE Terrestrial
Manual,2017 Chapter
2.1.15
1011 38affected tissues, internal
organs,milk,
Microbiological searching for bacterial
pathogens Pasteurella multocida/Mannheimia
haemolytica, Staphylococcus spp.,
Streptococcus spp., Pseudomonas spp.,
Escherichia coli, Klebsiella spp., Enterobacter
spp.
Culture, isolation, identification
P.J.Quin, M.E. Carter,B.K.
Markey, G.R. Carter, Clinical
Veterinary Microbiology,
WOLFE, 1994.
1012 39 small ruminans milkContagious agalactia in small ruminants
(detection of Mycobplasma agalactiae)Culture, stereoscopy, isolation, identification
OIE Terrestrial Manual
2013 Chapter 2.7.4.1013 40 Faeces Detection of oocysts in parasitic infections Teleman sedimentation method
1014 41 Lung tissue,milk Detection of Mycoplasma spp Culture, microscopyOIE Terrestrial Manual
2013 Chapter 2.7.4.
1015 42 Cultures AntibiogramsAntimicrobial Susceptibility–Agar Disk
Diffusion Test according to the valid editions
of CLSI:Μ2, CLSI:M100, Eucast
CLSI M2 M100 EUCAST
1016 43Apis mellifera honey bees and
broodVarroa destructor infestation
Macroscopic & microscopic examination and
characterization / OIE Terrestrial Manual
2014 2.2.7
OIE Terrestrial Manual
2008 Chapter 2.2.7.
1017 44 Apis mellifera honey beesDetection of Nosema spp. (spores) (qualitative
determination)
Microscopic examination and detection of
spores
OIE Terrestrial Manual
2013 Chapter 2.2.4.
1018 45 Apis mellifera honey bees AcarapisosisMacroscopic & microscopic examination and
characterization
OIE Terrestrial Manual
2014 Chapter 2.2.1.
1019 46 Honey bees, brood Detection of Melissococcus plutonius (EFB) Culture-microscopy OIE Terrestrial Manual
2016 Chapter 2.2.3.
1020 47 Honey bees, brood Detection of Paenibacillus larvae (AFB) Culture-microscopy / OIE Terrestrial O.I.E. Terrestrial Manual
2014 Chapter 2.2.21021 48 Brood Detection of Ascosphaera apis Macroscopic observation & microscopy Beebook, Coloss
1022 49 Brood Detection of Aspergillus flavus Macroscopic observation & microscopy Beebook, Coloss
1023 50 Adults, larvae Detection of Galleria mellonella ,Achroia grisella Macroscopic observation & stereoscopy Beebook, Coloss
1024
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1025
1026 Β.8 DVCTh/DEPARTMENT: VETERINARY LABORATORY OF LARISSA1027
1028 6th km of Larissa-Trikala highway, PC 411 10, Larissa1029 tel:+30 2410 617980, fax: +30 2410 6179821030 [email protected]
1032 NATIONAL REFERENCE LABORATORY
1033 #
1034 #1035 #1036 #
1037 #
1038
1039 OFFICIAL LABORATORY/LABORATORY DESIGNATED TO CARRY OUT ANALYSES WITHIN THE FIELD OF:1040 #
1041 #
1042 # Bovine leucosis Legal basis: Directive 64/432/ΕEC, Ministerial Desicion 286861/31.12.2003, Circular document no 218465/17.03.20041043
1044 ACCREDITED TESTS PERFORMED1045
1046 Tested materials/ products Types of test/ Properties to be measured Applied Standards/ Techniques to be used Documentation Certificate number Valid until
1047 1
Nervous tissue (obex) of
bovine, ovine and caprine
originDetection of the protein PrPRes
Immuno-enzymatic technique ΕLΙSΑ (TeSeE
BIO- RAD) according to the Commission
Regulation 999/2001/EC
Reg999/2001 ESYD 834-2 17/9/2020
1048 2
Nervous tissue (obex) of
bovine, ovine and caprine
originDetection of the protein PrPRes
Immunoblot test (Confirmatory Western blot
BIO-RAD) according to the Commission
Regulation 999/2001/EC
Reg999/2001 ESYD 834-2 17/9/2020
1049 3Nervous tissue (obex) of ovine
and caprine origin
Discriminatory test for strain typing of
transmissible spongiform encefalopathies in
small ruminants
Discriminatory test (Discriminatory CEA
METHOD BIO-RAD) according to the
Commission Regulation 36/2005/EC and the
«Discriminatory Testing Handbook of AHVLA
EU community reference laboratory for TSE
as amended
Reg36/2005 ESYD 834-2 17/9/2020
1050 4
Blood serum samples of
domestic or wild ruminants,
equine, swine, camelids,
carnivores and human origin
Detection of antibodies against Βrucella
abortus/ melitensis/ suis
Rose Bengal Test (R.B.) according to the
Commission Regulation 535/2002/EC
amending Annex C of the Directive
64/432/EEC and the Decision 2000/330/EC
Reg535/2002 ESYD 834-2 17/9/2020
1051 5
Blood serum samples of
domestic or wild ruminants,
equine, swine, camelids,
carnivores and human origin
Detection of antibodies against Βrucella
abortus/ melitensis/ suis
Complement Fixation Test (C.F.T.) according
to the Commission Regulation 535/2002/EC
amending Annex C of the Directive
64/432/EEC and the Decision 2000/330/EC
Reg535/2002 ESYD 834-2 17/9/2020
1052 6 Milk Detection of Campylobacter spp. ΕLΟΤ ΕΝ ISO 10272-1:2006 Reg854/2004 ESYD 641-2 1/12/2017
1053 7Milk and milk products, meat
and meat productsDetection of Salmonella spp. ΕLΟΤ ΕΝ ISO 6579:2002 Reg2073/2005 ESYD 641-2 1/12/2017
1054 8Milk and milk products, meat
and meat productsDetection of Listeria monocytogenes ΕLΟΤ ΕΝ ISO 11290-1:1996/Amd 1:2004 Reg2073/2005 ESYD 641-2 1/12/2017
1055 9 Raw cow milk Enumeration of somatic cellsMicroscopic method (Reference method) ISO
13366-1/IDF 148-1:2008Reg853/2004 ESYD 641-2 1/12/2017
1056
Zoonoses & zoonotic agents (Brucelloses)
Bovine Spongiform Encephalopathy & Small ruminants Transmissible
Cambylobacter
Milk and milk products (criteria for raw milk)
Total water content of poultry meat
Legal basis: Directives 64/432/EEC & 91/68/EEC, Ministerial Decisions 4224/120089/04.11.2015(B'2464) & 3339/117339/20.10.2016(B'3589)
Legal basis: Regulation (ΕC) 999/2001, Regulation (ΕU) 1148/2014
Legal basis: Ministerial Desicion 3865/128713/22.10.2013
Legal basis: Ministerial Desicion 3908/139386/14.12.2015 (Β'2833)
Legal basis: Regulation (EC) 543/2008, Ministerial Decision 327688/12.12.2005
Microbiological criteria for foodstuffs
Honeybee health
Legal basis: Reguation (EC)2073/2005, Presidential Decree 97/2017 (Α'138)
Legal basis: Ministerial Decision 4424/137557/11.11.2013(Β'2923)
Σελίδα 47 από 50
1057 NON-ACCREDITED TESTS PERFORMED
1058
1059 Tested materials/ products Types of test/ Properties to be measured Applied Standards/ Techniques to be used Documentation Comments
1060 10 MilkEnumeration of the total aerobic
microorganisms at 30oC ISO 4833-1:2013 Reg853/2004
1061 11Ovine nervous tissue
Ovine blood samples
Genotyping analysis of polymorphisms in the
PrP protein gene codons 136, 141, 154 and 171
to determine resistance to Scrapie Disease.
Multiplex RT-PCR Melting Curve Genotyping
using specific fluorescent
probesReg999/2001
1062 12 Bovine milk samples Antibody detection against Brucella spp.
ELISA Test according to the Commission
Regulation 535/2002/EC amending Annex C
of the Directive 64/432/EEC and the
Decision 2000/330/EC
Καν535/2003
1063 13 Bovine blood serum samples Antibody detection against Brucella spp. Indirect ELISA Καν535/2003
1064 14 Bovine blood serum, milk Antibody detection against Enzootic Bovine
Leucosis
BLV gp51 Ab Test, Screening format, Kit
Svanova & BLV gp51 Ab Test, Confirmation
format, Kit Svanova.
Direct.64/432/ΕΟΚ, Dis.
2009/976/ΕΕ
1065 15 Apis mellifera honey bees and
brood
Varroa destructor infestation. Macroscopic-
microscopic examination and characterization.
Macroscopic-microscopic examination and
characterization of Varroa destructor.
OIE Terrestrial Manual
2008 Chapter 2.2.7.
1066 16 Apis mellifera honey bees Nosema diagnosisAverage Nosema sp spore count per sample
in a haemacytometer.
OIE Terrestrial Manual
2013 Chapter 2.2.4.
1067 17Control of REV-1 & RB-51
Vaccines
Detection of Βrucella melitensis REV-1strain
& Brucella Abortus RB-51 strainCulture Isolation Identification Enumeration.
Manual of diagnostic Test
and Vaccines for
TERRESTRIAL Animals
1068 18Aborted tissue of domestic
animals
Detection of Βrucella spp, Cambylobacter spp,
Salmonella spp, Culture Isolation Identification
Manual of diagnostic Test
and Vaccines for
TERRESTRIAL Animals
2013 Chapter 2.4.3
and2.7.2 Techniques for
Brucellosis laboratory G.G.
ALTON/L.M.JONES
R.D.ANGUS/J.M.VERGER.ΕΝ
ΙSO 10272-1:2017 EN ISO
6579:2002
1069 19Blood smears of domestic
animalsDetection of all parasites
Microscopic examination - Giemsa stain-May
Grunwald stain
O.I.E. Terrestrial Manual
(Protocol and reagents
depending on the parasite)
1070 20Faecal smears of domestic
animals Detection Cryptosporidium spp oocysts
Microscopic examination - Modified Ziehl-
Neelsen
OIE Terrestrial Manual
2016 Chapter 2.9.4.
1071 21Lymphnotes and intestinal
smaears od domestic animals
Detection of Mycobacterium Avium subsp.
Paratuberculosis
Microscopic examination - Modified Ziehl-
Neelsen
OIE Terrestrial Manual
2014 Chapter 2.1.15.
1072 22Animal Tissue and milk from
domestic animals
Isolation of Pasteurella
multocida/Mannheimia haemolytica,
Staphylococcus spp., Streptococcus spp.,
Pseudomonas spp., Escherichia coli, Klebsiella
spp., Enterobacter spp. Salmonella spp,
Clostridium Spp
Culture, Isolation Identification
P.J.Quin, M.E. Carter,B.K.
Markey, G.R. Carter, Clinical
Veterinary Microbiology,
WOLFE, 1994.
1073 23 Milk, Lung tissue Detection of Mycoplasma spp. Culture, Stereoscopy, IdentificationOIE Terrestrial Manual
2013 Chapter 2.7.4.
1074 24 Culture of isolated bacteria Disk-Antibiogram (CLSI M2 M100 EUCAST). CLSI M2 M100 EUCAST
1075 25 FoodEnumeration of the total aerobic
microorganisms at 30oCISO4883-1:2013 Καν2073/2005
1076 26 FoodEnumeration of coagulase positive
staphylococciISO 6888-1:1999 Καν2073/2005
1077 27 FoodEnumeration of β-glucoronidase positive
Escherichia coliISO 16649-2:2001 Καν2073/2005
1078 28 Poultry meat Water content
Determination of nitrogen content (ISO
937:1978) Determination of water content
(ISO 1442:1997)
Reg1538/1991
1079 29 Raw milk Determination of albendazole sulfoxideInternal method (Journal of AOAC
International, Vol. 79, No. 6, 1996)Reg37/2010, Reg470/2009
1080
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1081
1082 Β.9 DVCTh/BIOLOGICAL PRODUCTS, DIAGNOSTIC PATHOLOGY & PARASITOLOGY OFFICE1083
1084 80, 26th Oktovriou Street, 546 27 Thessaloniki1085 tel:+30 210 566063, fax:+30 210 5520231086 [email protected]
1088 OFFICIAL LABORATORY/LABORATORY DESIGNATED TO CARRY OUT ANALYSES WITHIN THE FIELD OF:1089 #
1090
1091 ACCREDITED TESTS PERFORMED1092
1093
Tested materials/ products Types of test/ Properties to be measured Applied Standards/ Techniques to be used
Documentation Certificate number Valid until
1094 1
Bovine tissues (Lymph nodes:
retropharyngeal, bronchial,
mediastinal and portal. Lung,
liver, spleen and the surfaces
of body cavities-diaphragm)
Histological method for tubercle's detection Haematoxyline - Eosin staining for tubercle's
detection
Council Directive
64/432/EEC, PD 308/2000,
MD 30/3430/14.01.15
ESYD 844-2 21/11/2020
1095
1096 NON-ACCREDITED TESTS PERFORMED1097
1098 Tested materials/ products Types of test/ Properties to be measured Applied Standards/ Techniques to be used Documentation Comments
1099 2Tissues of goats ,sheep and
petsHistological method for tubercle's detection
Haematoxyline - Eosin staining for tubercle's
detection
Council Directive
64/432/EEC, PD 308/2000,
MD 30/3430/14.01.15,
Wangoo et al (2005)
J.Comp.Path.,133,223-234
Validated
1100 3 Lungs of goats and sheepHistological method for detection of maedi
pneumonia Haematoxyline - Eosin staining
Pathology of Domestic
Animals, Jubb, Kennedy &
Palmer's, Elsevier
Saunders, 2007
1101 4 Lungs of goats and sheep Histological method for detection of parasitesHaematoxyline - Eosin staining for detection
of parasitic bronchopneumonia
Pathology of Domestic
Animals, Jubb, Kennedy &
Palmer's, Elsevier
Saunders, 2007
1102 5 Lungs of goats and sheepHistological method for detection of
Mannhemia spp pneumonia Haematoxyline - Eosin staining
Pathology of Domestic
Animals, Jubb, Kennedy &
Palmer's, Elsevier
Saunders, 2007
1103 6 Lungs and pleura of goats Histological method for detection of infectious
pleuropneumoniaHaematoxyline - Eosin staining
Pathology of Domestic
Animals, Jubb, Kennedy &
Palmer's, Elsevier
Saunders, 2007
1104 7 Brain of goats and sheep Histological method for visna -maedi detection Haematoxyline - Eosin staining
Pathology of Domestic
Animals, Jubb, Kennedy &
Palmer's, Elsevier
Saunders, 2007
1105 8 Brain of goats and sheep Histological method for Listeria detection Haematoxyline - Eosin staining
Pathology of Domestic
Animals, Jubb, Kennedy &
Palmer's, Elsevier
Saunders, 2007
1106 9 Brain of goats and sheepHistological method for the detection of
embolic granulomatous meningoencephalitisHaematoxyline - Eosin staining
Pathology of Domestic
Animals, Jubb, Kennedy &
Palmer's, Elsevier
Saunders, 2007
1107 10 Brain of goats and sheepHistological method for the detection of
parasitic encephalitisHaematoxyline - Eosin staining
Pathology of Domestic
Animals, Jubb, Kennedy &
Palmer's, Elsevier
Saunders, 2007
1108 11 Brain of goats and sheepHistological method for the detection of VitA
deficiencyHaematoxyline - Eosin staining
Pathology of Domestic
Animals, Jubb, Kennedy &
Palmer's, Elsevier
Saunders, 2007
Zoonoses & zoonotic agents (Bovine Tuberculosis) Legal basis: Ministerial Decision 30/3430/14.01.2015(B'171)
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1109 12Mucosa of nose cavities of
sheep
Histological method for the detection of
endemic intramucosal neoplasia of the noseHaematoxyline - Eosin staining
Pathology of Domestic
Animals, Jubb, Kennedy &
Palmer's, Elsevier
Saunders, 2007
1110 13 Lips skin of the sheepHistological method for the detection of
contagious ecthyma Haematoxyline - Eosin staining
Pathology of Domestic
Animals, Jubb, Kennedy &
Palmer's, Elsevier
Saunders, 2007
1111 14 Skin of goats and sheepHistological method for the detection of
sarcoptic,psoroptic and chorioptic scabiesHaematoxyline - Eosin staining
Pathology of Domestic
Animals, Jubb, Kennedy &
Palmer's, Elsevier
Saunders, 2007
1112 15 Mammary gland of sheepHistological method for the detection of mixed
infection by Maedi-Corynebacterium sppHaematoxyline - Eosin staining
Pathology of Domestic
Animals, Jubb, Kennedy &
Palmer's, Elsevier
Saunders, 2007
1113 16 Mammary gland of cowHistological method for the detection of
gangrenous mastitisHaematoxyline - Eosin staining
Pathology of Domestic
Animals, Jubb, Kennedy &
Palmer's, Elsevier
Saunders, 2007
1114 17 Tissues of productive animalsHistological method for the detection of
neoplasiasHaematoxyline - Eosin staining
Tumors in Domestic
Animals 4th ed.Meuten
Editor Blackwell ,2002
1115 18 Tissues of rabbit Histological method for the detection of
myxomatosisHaematoxyline - Eosin staining
Pathology of Domestic
Animals, Jubb, Kennedy &
Palmer's, Elsevier
Saunders, 2007
1116 19 Meat of slaughetered bovineHistological method for the detection of
eosinophilic myiositisHaematoxyline - Eosin staining
Pathology of Domestic
Animals, Jubb, Kennedy &
Palmer's, Elsevier
Saunders, 2007
1117 20 Poultry LiverHistological method for the detection of
hepatitis with inclusion bodies (adenovirus)
and mycotoxicosis
Haematoxyline - Eosin stainingDiseases of Poultry 10th
Ed.B.W.Calnek,Mosby-
Wolfe,1997
1118 21 Tissues of laying chickens Histological method for the detection of Marek
diseaseHaematoxyline - Eosin staining
Diseases of Poultry 10th
Ed.B.W.Calnek,Mosby-
Wolfe,1997
1119 22Tissues of laboratory animals
(white mice)
Istological examination within the framework
of the European and Greek legislationHaematoxyline - Eosin staining
PD56/2013, Reg15/2011,
Digestive System,
Monographs In Pathology
of Laboratory Animals,Ed.
T.C. Jones ,U.Mohr,
R.D.Hunt, Springer
Verlag,1985
1120 23Tissues of laboratory animals
(white mice)Istological examination for neoplasias Haematoxyline - Eosin staining
Pathology of Neoplasia and
Preneoplasia in Rodents
EULEP Color Atlas, Bannash
and
Gossner,Schattauer,1994
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