Unrivaled Life Sciences - Fisher Scientific...Corning Spheroid Microplate, 384 Well, Black with...

24
Cell Culture Protein Biology Molecular Biology Unrivaled Life Sciences

Transcript of Unrivaled Life Sciences - Fisher Scientific...Corning Spheroid Microplate, 384 Well, Black with...

Page 1: Unrivaled Life Sciences - Fisher Scientific...Corning Spheroid Microplate, 384 Well, Black with Clear Round-Bottomed Wells, Ultra-Low Attachment (ULA) Coating, with Lid, Sterile 5/Case

Cell Culture Protein Biology Molecular Biology

Unrivaled

Life Sciences

Page 2: Unrivaled Life Sciences - Fisher Scientific...Corning Spheroid Microplate, 384 Well, Black with Clear Round-Bottomed Wells, Ultra-Low Attachment (ULA) Coating, with Lid, Sterile 5/Case

Visit fishersci.com/UnrivaledLifeSciences or fishersci.ca/UnrivaledLifeSciences to see all featured products.

The Fisher Scientific channel gives you access to a range of new life sciences products, exclusive new savings, and a variety of life sciences product essentials. Find best-in-class cell culture, protein biology, and molecular biology solutions from the most trusted brands in the industry. Plus, save money on your most crucial and trustedlife sciences products.

Find exclusive savings on new, leading-edge products!

Page 3: Unrivaled Life Sciences - Fisher Scientific...Corning Spheroid Microplate, 384 Well, Black with Clear Round-Bottomed Wells, Ultra-Low Attachment (ULA) Coating, with Lid, Sterile 5/Case

Applied Biosystems, B-Per, BCA, Bolt, CloneWell, Countess, E-Gel, FASTRun, Fisher BioReagents, Halt, High-Select, iBind, iBlot, iBright, Invitrogen, M-PER, NE-PER, Pierce, Quant-iT, Qubit, SeeBlue, Smart Exposure, SuperSignal, T-PER, Thermo Scientific and TMT10plex are trademarks of Thermo Fisher Scientific Inc. or its subsidiaries. BioCoat, CellBIND, CoolBox, CoolRack, Corning, Costar, Falcon, Fluoroblok, HYPERFlask, Matrigel and Transwell are trademarks of Corning Inc.

Page 5: Unrivaled Life Sciences - Fisher Scientific...Corning Spheroid Microplate, 384 Well, Black with Clear Round-Bottomed Wells, Ultra-Low Attachment (ULA) Coating, with Lid, Sterile 5/Case

4

Cell Culture

fishersci.com/LifeSciences | fishersci.ca/LifeSciences

New

Description Quantity Cat. No. Transwell Permeable Supports, 6.5mm PET Membranes, 0.4µm, Sterile 48/Case 07-200-154

Transwell Permeable Supports, 6.5mm PC Membranes, 8µm, Sterile 48/Case 07-200-150

Transwell Permeable Supports, 12mm PET Membranes, 0.4µm, Sterile 48/Case 07-200-156

HTS Transwell-24 Permeable Supports, PET Membranes, 0.4µm, Sterile 12/Case 07-200-539

HTS Transwell-96 Permeable Supports, PET Membranes, 0.4µm, Sterile 5/Case 12-456-723

HTS Transwell-96 Permeable Supports, PET Membranes, 1µm, Sterile 5/Case 07-200-742

Corning BioCoat Collagen I Inserts, PET Membranes, in 24-Well Falcon Companion Plates, 0.4μm, Sterile 24/Case 08-774-19

Falcon Permeable Supports, for 6-Well Plates, Transparent PET Membranes, 3µm, Sterile, Individually Wrapped 48/Case 08-771-3

Corning FluoroBlok Cell Culture Inserts, for 24-Well Plates, FluoroBlok PET Membranes, 8μm, Sterile 48/Case 08-772-142

Corning BioCoat Tumor Invasion 24-Well Plates, Coated FluoroBlok PET Membranes, 8μm, Sterile 5/Case 08-774-381

Corning Transwell and Falcon Permeable SupportsTranswell and Falcon permeable supports are widely used for complex 2D and 3D cell culture models, such as multi-layered tissues, migration/invasion assays, and co-culture applications. Corning permeable supports are available in a variety of formats, pore sizes and membrane types.

Features

• Produce a more in vivo-like cell culture environment suitable for sophisticated 3D models

• Allow polarized cells to conduct metabolic activities more naturally via both apical and basolateral feeding

• Co-culture cells with or without cell-to-cell contact

Page 6: Unrivaled Life Sciences - Fisher Scientific...Corning Spheroid Microplate, 384 Well, Black with Clear Round-Bottomed Wells, Ultra-Low Attachment (ULA) Coating, with Lid, Sterile 5/Case

5 Unrivaled Life Sciences fishersci.com/UnrivaledLifeSciences | fishersci.ca/UnrivaledLifeSciences

New

Description Quantity Cat. No.

Corning Spheroid Microplate, 96 Well, Black with Clear Round-Bottomed Wells, Ultra-Low Attachment (ULA) Coating, with Lid, Sterile

5/Case 12-456-721

50/Case 15-100-173

Corning Spheroid Microplate, 384 Well, Black with Clear Round-Bottomed Wells, Ultra-Low Attachment (ULA) Coating, with Lid, Sterile

5/Case 12-456-722

50/Case 15-100-172

Corning Spheroid Microplates Corning Spheroid Microplates are an ideal tool for generating, culturing, and assaying single 3D multicellular spheroids in the same microplate. The combination of the Corning Ultra-Low Attachment (ULA) Surface on the well bottom and an innovative well geometry enables highly reproducible growth of 3D cell spheroid cultures. Automation-friendly formats (96- and 384-wells) make it easier to generate spheroid models in a format suitable for high-throughput screening (HTS) platforms.

Features

• Clear, round well bottoms with black opaque microplate well bodies

• Covalently attached ULA surface to reduce cellular adhesion

• Novel and proprietary well geometry — aids spheroid formation in the well center

• Unique design minimizes well-to-well crosstalk

Description Quantity Cat. No. Corning TC-Treated Culture Dish, 100mm 500/Case 08-772-22

Costar 6-Well TC-Treated Cell Culture Plate, Clear, Sterile 50/Case 07-200-83

Falcon 6-Well TC-Treated Cell Culture Plate, Clear, Sterile 50/Case 08-772-1B

Falcon 12-Well TC-Treated Cell Culture Plate, Clear, Sterile 50/Case 08-772-29

Corning Cell Culture Dishes and PlatesCorning’s innovative cell culture dishes and plates provide superior cell attachment, growth and visibility. Visit fishersci.com/CorningCellCultureSolutions or fishersci.ca/CorningCellCultureSolutions to view them all.

Page 7: Unrivaled Life Sciences - Fisher Scientific...Corning Spheroid Microplate, 384 Well, Black with Clear Round-Bottomed Wells, Ultra-Low Attachment (ULA) Coating, with Lid, Sterile 5/Case

6

Cell Culture

fishersci.com/LifeSciences | fishersci.ca/LifeSciences

New

Description Quantity Cat. No. Corning Matrigel GFR* Basement Membrane Matrix, Phenol Red Free, LDEV± Free 10mL CB-40230C

Corning Matrigel GFR* Basement Membrane Matrix, LDEV± Free 10mL CB-40230

Corning Matrigel hESC-Qualified Matrix, LDEV± Free 10mL 08-774-552

Corning Matrigel Basement Membrane Matrix, LDEV± Free 5mL CB-40234

Corning Matrigel Matrix HC**, Phenol Red Free, LDEV± Free 10mL 08-774-391

Corning Matrigel Matrix HC**, GFR*, LDEV± Free 10mL 08-774-392

Corning Matrigel Basement Membrane Matrix HC**, LDEV± Free 10mL CB354248

Corning Collagen I, Rat Tail 10mL CB-40236

Corning Collagen I, HC**, Rat Tail 100mg CB354249

Corning Laminin/Entactin, HC* 100mg 08-774-555

Corning Matrigel, GFR* Basement Membrane Matrix, Phenol Red Free, LDEV± Free 10.5mg CB-40230C

Corning Matrigel for 3D Cell Culture Corning Matrigel Matrix, a hydrogel based on an extracellular matrix (ECM), is suited to 3D and organoid cell culture and to other cell types and functions. Matrigel is a reconstituted basement membrane extract from Engelbreth-Holm-Swarm (EHS) mouse tumors, and it contains the prominent cb-40230cECM molecules found in basement membrane. These components promote cellular viability, proliferation, function, and development of many cell types, as well as subsequent cellular responses that are more physiologically relevant (compared to cells grown in 2D environments). Corning also offers other natural ECMs, including collagen, laminin and fibronectin.

Features

• Well-suited for culturing polarized cells (such as epithelial cells)• Promotes the differentiation of hepatocytes, neurons,

mammary epithelial, endothelial, smooth muscle cells, and many other cell types

• May be used to assess the in vivo angiogenic activity of different compounds

• Has promoted the successful transplantation of prostatic, breast, small cell lung and colon tumors; adrenal carcinomas; melanomas; lymphoblastic leukemia cells; and many other human cancer cells

GFR* = Growth Factor Reduced HC** = High Concentration LDEV ± = Lactose Dehydrogenase Elevating Virus

Page 8: Unrivaled Life Sciences - Fisher Scientific...Corning Spheroid Microplate, 384 Well, Black with Clear Round-Bottomed Wells, Ultra-Low Attachment (ULA) Coating, with Lid, Sterile 5/Case

7 Unrivaled Life Sciences fishersci.com/UnrivaledLifeSciences | fishersci.ca/UnrivaledLifeSciences

Description Quantity Cat. No. Falcon 5-Layer Multi-Flask, 875cm2, Rectangular, Straight Neck, Vented Cap 8/Case 14-826-96

Falcon 3-Layer Multi-Flask, 525cm2, Rectangular, Straight Neck, Vented Cap 12/Case 14-826-95

Description Quantity Cat. No. HYPERFlask M, CellBIND-Treated, for Manual Use, 1720cm2 Growth Area 4/Case 09-761-22

HYPERFlask M, CellBIND-Treated, for Manual Use, 1720cm2 Growth Area 4/Pack; 24/Case 13-700-420

HYPERFlask M, CellBIND-Treated, for Manual Use, 1720cm2 Growth Area 1/Pack; 4/Case 09-761-23

HYPERFlask, CellBIND-Treated, for Use with The Automation Partnership (TAP) SelecT™ and CompacT SelecT™ Automated Cell Culture Systems

4/Pack; 24/Case 13-700-407

Falcon Multi-FlasksFalcon Multi-Flasks have the same footprint and seeding densities as single-layer T-175 flasks, but feature three- and five-layer designs that provide greater surface area and cell yield so you can easily scale-up existing protocols.

Features

• Even media distribution across all layers for homogeneous cell growth

• Mix cells and reagents to save time and reduce contamination risk

• Pour or recover cells using a pipet• Consistent surface treatments for predictable scale-up• Compliant with cGMP manufacturing standards

Corning HYPERFlask The Corning HYPERFlask Cell Culture Vessel (high-yield performance flask) offers a 1720cm2 growth area in the footprint of a traditional 175cm2 flask. This flask has a multilayered, gas-permeable growing surface for efficient gas exchange.

Features

• Ten interconnected polystyrene growth surfaces• Superior cell attachment and growth with the gas-

permeable polystyrene Corning CellBIND surface • Bar coded — choose from manual or automation-

friendly versions

Page 9: Unrivaled Life Sciences - Fisher Scientific...Corning Spheroid Microplate, 384 Well, Black with Clear Round-Bottomed Wells, Ultra-Low Attachment (ULA) Coating, with Lid, Sterile 5/Case

8

Cell Culture

fishersci.com/LifeSciences | fishersci.ca/LifeSciences

Description Quantity Cat. No. Corning CoolBox

CoolBox XT, Holder with One Cooling Core, Orange 1/Case 07-210-023

CoolBox 2XT, Holder with Two Cooling Cores, Orange 1/Case 07-210-027

Corning CoolRack

CoolRack 15mL, Holds 9 x 15mL Centrifuge Tubes (Tall) 1/Case 07-210-061

CoolRack 50mL, Holds 4 x 50mL Centrifuge Tubes (Tall) 1/Case 07-210-062

CoolRack M30, Holds 30 x 1.5/2mL Microtubes 1/Case 07-210-041

CoolRack XT PCR96, Holds 96-Well Plate, 12 Strip Wells, or 96-200µL Tubes 1/Case 07-210-053

CoolRack CFT24, Holds 24 Cryogenic Vials or FACS Tubes 1/Case 07-210-050

CoolRack CF45, Holds 45 Cryogenic Vials or FACS Tubes 1/Case 07-210-051

Corning CoolRack and CoolBoxCorning CoolRack and CoolBox solutions support consistent, reproducible, and standardized sample handling. They address the pitfalls of temperature regulation and reduce contamination risk, keeping your samples cool and stable while you work. Automation-compatible options are also available.

Use CoolRack and CoolBox products together to:

• Work completely ice free• Reduce temperature variability • Realize consistent, reproducible, and standardized

temperature control for sample handling

Invitrogen Automated Cell CountersThermo Fisher Scientific offers two high-performance automated cell counters to meet any lab’s needs. Choose simple brightfield counting with the Invitrogen Countess II Automated Cell Counter, or the flexibility and upgradability of fluorescence detection with the Invitrogen Countess II FL Automated Cell Counter.

Features

• Accuracy — eliminate the subjectivity and user-to-user ariability of manual cell counting• Speed — count living and dead cells, report on viability, and measure average cell size in as little as 10 seconds• Convenience — the touchscreen and simple user interface provide quick startup and require minimal training

Description Cat. No. Invitrogen Countess II Automated Cell Counter AMQAX1000

Invitrogen Countess II FL Automated Cell Counter AMQAF1000

Countess II FL Reusable Slide A25750

Countess Cell Counting Chamber Slides, Case of 500 C10312

EVOS Light Cube, RFP (Red Fluorescent Protein) AMEP4652

EVOS Light Cube, GFP (Green Fluorescent Protein) AMEP4651

Page 10: Unrivaled Life Sciences - Fisher Scientific...Corning Spheroid Microplate, 384 Well, Black with Clear Round-Bottomed Wells, Ultra-Low Attachment (ULA) Coating, with Lid, Sterile 5/Case

9 Unrivaled Life Sciences fishersci.com/UnrivaledLifeSciences | fishersci.ca/UnrivaledLifeSciences

Protein BiologyOur newest Western blotting solutions, buffers and kits improve the quality of your data, increase lab productivity and make detecting, measuring and analyzing protein expression, identity and function quick and easy.

Visit fishersci.com/UnrivaledLifeSciences or fishersci.ca/UnrivaledLifeSciences to learn more.

Page 11: Unrivaled Life Sciences - Fisher Scientific...Corning Spheroid Microplate, 384 Well, Black with Clear Round-Bottomed Wells, Ultra-Low Attachment (ULA) Coating, with Lid, Sterile 5/Case

10

Protein Biology

fishersci.com/LifeSciences | fishersci.ca/LifeSciences

Fisher BioReagents FASTRun Tris SDS PAGE Running Buffer,10xIncrease your lab productivity, save money and cut your time to results by up to 70% with FASTRun Tris SDS PAGE Running Buffer, 10x.

Features

• Comparable or better resolution and a broader MW separation range (compared to traditional Tris-Glycine-SDS buffer); fewer TG gel compositions to fully resolve proteins

• Compatible with standard polyacrylamide gels (premade or pour your own)

• Works with all commercially available protein electrophoresis tanks

See the Difference

We tested FASTRun Buffers against traditional buffers, and the results were undeniable. FASTRun Buffers delivered clear separation of protein bands in as little as 20 minutes, while traditional buffers took 90 minutes to finish running. Plus, FASTRun Buffers showed gradient-like separation on a single concentrated gel, with impressive clarity even at a wide range of molecular weights.

Visit fishersci.com/FastRunSDSBuffer or fishersci.ca/FastRunSDSBuffer to watch the video and read our white paper.

Description Quantity Cat. No. Tris SDS PAGE Running Buffer, 10X 500mL BP881-500

Tris SDS PAGE Running Buffer, 10X 1L BP8811

Page 12: Unrivaled Life Sciences - Fisher Scientific...Corning Spheroid Microplate, 384 Well, Black with Clear Round-Bottomed Wells, Ultra-Low Attachment (ULA) Coating, with Lid, Sterile 5/Case

11 Unrivaled Life Sciences fishersci.com/UnrivaledLifeSciences | fishersci.ca/UnrivaledLifeSciences

Description Cat. No. Protein Extraction Essentials Bundles

Mammalian Cell Protein Extraction Bundle

• M-PER Mammalian Protein Extraction Reagent, 250mL (Cat. No. PI78501)• Pierce Protease Inhibitor Mini Tablets, 30 Tablets (Cat. No. A32953)• Pierce Rapid Gold BCA Protein Assay Kit, 250mL (Cat. No. A53226)

PIB90001

Tissue Protein Extraction Bundle

• T-PER Tissue Protein Extraction Reagent, 500mL (Cat. No. PI78510)• Pierce Protease Inhibitor Mini Tablets, 30 Tablets (Cat. No. A32953)• Pierce Rapid Gold BCA Protein Assay Kit, 250mL (Cat. No. A53226)

PIB90002

Nuclear and Cytoplasmic Protein Extraction Bundle

• NE-PER Nuclear and Cytoplasmic Extraction Reagents, 15mL (Cat. No. PI78833)• Halt Protease Inhibitor Cocktail (100X), 1mL (Cat. No. PI87786)• Micro BCA Protein Assay Kit, 500mL (Cat. No. PI23235)

PIB90003

RIPA Protein Extraction Bundle

• RIPA Lysis and Extraction Buffer, 100mL (Cat. No. PI89900)• Halt Protease Inhibitor Cocktail (100X), 1mL (Cat. No. PI87786)• Pierce Rapid Gold BCA Protein Assay Kit, 250mL (Cat. No. A53226)

PIB90004

Protein Purification Essentials Bundles

Antibody Purification Bundle

• Pierce Protein A/G Magnetic Agarose Beads, 1mL (Cat. No. PI78609)• Pierce IgG Elution buffer, pH 2.0, 1L (Cat. No. PI21028)• Pierce Protein A/G IgG Binding Buffer, 240mL (Cat. No. PI54200)

PIB90005

DYKD4K Magnetic Agarose Purification Bundle

• Pierce Anti-DYKDDDDK Magnetic Agarose, 1mL (Cat. No. A36797)• Halt Protease Inhibitor Cocktail (100X), 1mL (Cat. No. PI87786)• Pierce IP Lysis Buffer, 100mL (Cat. No. PI87787)• Pierce IgG Elution Buffer, 1L (Cat. No. PI21004)

PIB90006

GST Magnetic Agarose Purification Bundle

• Pierce Glutathione Magnetic Agarose Beads, 1mL (Cat. No. PI78601)• Halt Protease Inhibitor Cocktail (100X), 1mL (Cat. No. PI87786)• B-PER Complete Bacterial Protein Extraction Reagent, 250mL (Cat. No. PI89821)

PIB90007

His-Tag Magnetic Agarose Purification Bundle

• Pierce Ni-NTA Magnetic Agarose Beads, 1mL (Cat. No. PI78605)• Halt Protease Inhibitor Cocktail, EDTA-free (100X), 1mL (Cat. No. PI87785)• B-PER Complete Bacterial Protein Extraction Reagent, 250mL (PI89821)

PIB90008

Mass Spectrometry Sample Preparation Essentials Bundles

Accessing the Phosphoproteome Bundle

• High-Select TiO2 Phosphopeptide Enrichment Kit, 24 Reactions (Cat. No. A32993)• High-Select Fe-NTA Phosphopeptide Enrichment Kit, 30 Reactions (Cat. No. A32992)

PIB90009

Accessing the Proteome Bundle

• TMT10plex Isobaric Label Reagent Set, 3 x 0.8mg, 30 Reactions (Cat. No. PI90111)• Pierce™ High pH Reversed-Phase Peptide Fractionation Kit, 12 Reactions (Cat. No. PI84868)

PIB90010

Accessing the Plasma Proteome Bundle

• TMT10plex Isobaric Label Reagent Set, 3 x 0.8mg, 30 Reactions (Cat. No. PI90111)• High Select Top14 Abundant Protein Depletion Mini Spin Columns, 24 Columns (Cat. No. A36370)• Pierce Peptide Desalting Columns, 25 Columns (Cat. No. PI89852)

PIB90011

Mass Spectrometry Sample Preparation Bundle

• Pierce Trypsin Protease, MS Grade, 5 x 20µg (Cat. No. PI90057)• Pierce Lys-C Protease, MS Grade, 100µg (Cat. No. PI90307)• Pierce Peptide Desalting Spin Columns, 25 Columns (Cat. No. PI89852)• Pierce Quantitative Colorimetric Peptide Assay, 500 Assays (Cat. No. PI23275)

PIB90012

Save 20% with Thermo Scientific Protein Lab Essentials BundlesGet your protein biology workflows off to a great start with these protein lab essentials bundles, each of which contains validated, optimized reagents. By purchasing the bundle, you save 20% compared to purchasing the components individually.

New

Page 13: Unrivaled Life Sciences - Fisher Scientific...Corning Spheroid Microplate, 384 Well, Black with Clear Round-Bottomed Wells, Ultra-Low Attachment (ULA) Coating, with Lid, Sterile 5/Case

12

Protein Biology

fishersci.com/LifeSciences | fishersci.ca/LifeSciences

Thermo Scientific Chemiluminescent HRP Substrates

Description Quantity Cat. No. Pierce ECL Substrate 250mL Kit PI32209

Pierce ECL Substrate 500mL Kit PI32106

Pierce ECL 2 Western Blotting Substrate 100mL Kit PI80196

SuperSignal West Pico PLUS Chemiluminescent Substrate 200mL Kit PI34577

SuperSignal West Pico PLUS Chemiluminescent Substrate 500mL Kit PI34580

SuperSignal West Dura Extended Duration Substrate 100mL Kit PI34075

SuperSignal West Dura Extended Duration Substrate 200mL Kit PI34076

SuperSignal West Femto Maximum Sensitivity Substrate 100mL Kit PI34095

SuperSignal West Femto Maximum Sensitivity Substrate 200mL Kit PI34096

Thermo Scientific Pierce ECL and SuperSignal Chemiluminescent Substrates enable high sensitivity, long signal duration, strong signal intensity, and low background to bring excellent performance to your Western blotting applications. Choose the chemiluminescent HRP substrate that best suits your Western blot sensitivity needs from the Thermo Scientific Pierce ECL, Pierce ECL 2 and SuperSignal product families. Visit thermofisher.com/chemisubstrates to view the complete Western Blot Chemiluminescent Substrate Selection Guide.

Page 15: Unrivaled Life Sciences - Fisher Scientific...Corning Spheroid Microplate, 384 Well, Black with Clear Round-Bottomed Wells, Ultra-Low Attachment (ULA) Coating, with Lid, Sterile 5/Case

14

Protein Biology

fishersci.com/LifeSciences | fishersci.ca/LifeSciences

Description Quantity Cat. No. iBright FL1000 Imaging System (Fluorescent and Chemiluminescent) 1 Each A32752

iBright CL1000 Imaging System (Chemiluminescent) 1 Each A32749

Related Products

Invitrogen iBright Prestained Protein Ladder Compatible with chemiluminescent, chromogenic, and fluorescent detection.

2 x 250μL LC5615

Thermo Scientific SuperSignal West Pico PLUS Chemiluminescent Substrate Excellent sensitivity (low picogram to high femtogram), intensity, and signal duration (up to 24 hours) compared to other ECL substrates in its class. Compatible with different membranes, blocking reagents, and a wide range of antibody dilutions, making it an ideal choice for most Western blotting applications.

500mL PI34580

Invitrogen Secondary Antibodies Choose from our sensitive and verified HRP or fluorescent secondary antibodies, including our new Alexa Fluor Plus antibodies, designed to provide brighter signal and enhanced sensitivity.

Visit thermofisher.com/antibodies to find the right antibody for your research needs.

Invitrogen iBright Imaging SystemsThe new Invitrogen iBright CL1000 and FL1000 Imaging Systems deliver powerful image capture and analysis in a package that’s easy for users of all experience levels to operate. Whether you’re imaging chemiluminescent Western blots, fluorescent blots, or stained protein or nucleic acid gels, there’s an iBright imaging solution for you.

Features

• Powerful imaging — a robust 9.1-megapixel charge-coupled device (CCD) camera combined with proprietary Smart Exposure technology provides rapid determination of optimal exposure times

• Load and go — logical user interface, workflows, and advanced automated features streamline the imaging experience

• Bring your blots to life — capture up to four proteins in a single blot with the five fluorescence channels of the iBright FL1000 system

Visit thermofisher.com/iBright to learn more about iBright Imaging Systems.

New

Nucleic acid gels Protein gels Chemiluminescent blots Flourescent blots

Page 16: Unrivaled Life Sciences - Fisher Scientific...Corning Spheroid Microplate, 384 Well, Black with Clear Round-Bottomed Wells, Ultra-Low Attachment (ULA) Coating, with Lid, Sterile 5/Case

15 Unrivaled Life Sciences fishersci.com/UnrivaledLifeSciences | fishersci.ca/UnrivaledLifeSciences

Invitrogen iBright Imaging System and iWestern Benchtop BundleImprove your Western blot data quality while increasing your lab productivity when you pair the Invitrogen iBright Imaging System and iWestern Benchtop Bundle. These high-performance tools and innovative technologies modernize and simplify western blotting — and, for a limited time, you’ll save 20% on the iWestern Benchtop Bundle when you purchase them together.

Description Quantity Cat. No.

iWestern Benchtop Bundle Includes: Mini Gel Tank, 10-Well Bolt 4-12% Bis-Tris Plus Gel, Bolt MES SDS Running Buffer, Bolt LDS Sample Buffer, Bolt Sample Reducing Agent, SeeBlue Plus2 Pre-Stained Standard, iBlot 2 Gel Transfer Device, iBlot 2 Transfer Stacks, Regular Nitrocelluose, iBind Western Device, iBind Cards, iBind Solution Kit

Bundle IW3000S

New

Page 17: Unrivaled Life Sciences - Fisher Scientific...Corning Spheroid Microplate, 384 Well, Black with Clear Round-Bottomed Wells, Ultra-Low Attachment (ULA) Coating, with Lid, Sterile 5/Case

16

Protein Biology

fishersci.com/LifeSciences | fishersci.ca/LifeSciences

Life Sciences Samples

• Protein Assays & Analysis• Protein Purification & Isolation• Protein Gel Electrophoresis• Restriction Enzyme Cloning• PCR & qPCR Reagents• And more!

Visit fishersci.com/lifesamples or fishersci.ca/lifesamples to request your complimentary samples today.

Life Sciences Demos

Request your demo today for Thermo Scientific, Invitrogen and Applied Biosystems instruments to perform:

• PCR & qPCR• Cell Analysis & Imaging• Molecular and Protein Electrophoresis• Western Blotting

Visit fishersci.com/lifescidemos or fishersci.ca/lifescidemos to request a free demo today.

Samples and Demos

Samples and DemosPut these products to the test.

See the quality for yourself — test instruments and get samples from the Thermo Scientific, Invitrogen, Applied Biosystems, and Gibco brands.

Page 18: Unrivaled Life Sciences - Fisher Scientific...Corning Spheroid Microplate, 384 Well, Black with Clear Round-Bottomed Wells, Ultra-Low Attachment (ULA) Coating, with Lid, Sterile 5/Case

17 fishersci.com/UnrivaledLifeSciences | fishersci.ca/UnrivaledLifeSciencesUnrivaled Life Sciences

Our collection of molecular biology products helps you streamline your research with the latest technologies from the industry’s most trusted brands — including time-saving assays, ultrapure qPCR water, and ethanol solutions.

Visit fishersci.com/UnrivaledLifeSciences or fishersci.ca/UnrivaledLifeSciences to learn more.

Molecular Biology

Page 19: Unrivaled Life Sciences - Fisher Scientific...Corning Spheroid Microplate, 384 Well, Black with Clear Round-Bottomed Wells, Ultra-Low Attachment (ULA) Coating, with Lid, Sterile 5/Case

18

Molecular Biology

fishersci.com/LifeSciences | fishersci.ca/LifeSciences

Tested Contaminants ResultsDNase, RNase, Protease, Nickase Not Detected

Human Genomic DNA ≤ 0.3pg

E. coli DNA ≤ 0.03pg

Total Aerobic Microbial Count ≤ 1CFU/mL

Total Yeast and Mold Count ≤ 1CFU/mL

Endotoxins < 0.1EU/mL

Fisher BioReagents Ultrapure qPCR and RT-qPCR WaterNew Fisher BioReagents Ultrapure qPCR-Grade Water was developed specifically for quantitative PCR (qPCR) and quantitative reverse transcription PCR (RT-qPCR). It’s free of hydrolytic enzymes that can degrade the target nucleic acid and interfere with amplification, and it’s tested to ensure the absence of human genomic and E. coli DNA that can lead to false-positive signals, so you can be confident in your qPCR and RT-qPCR results.

The unique specifications of Fisher BioReagents Ultrapure qPCR-Grade Water will help you consistently produce high-quality, reliable results in your applications:

• RNAi or microarray validation • Genetic testing • Pathogen detection • Disease research • Gene expression analysis • Genotyping

Convenient sizes make this product suitable for everything from small-volume lab work to large-volume reagent preparation. Batch-to-batch consistency makes this water suitable for manufacturing master mixes and other water-based reagent and kit components. Choose from convenient sizes for everything from small-volume lab work to large-volume reagent preparation.

Features

• Uniquely tested for nucleic acid contamination (E. coli DNA, human genomic DNA)

• DNase, RNase, protease and nickase free• Sub-micron filtered (0.03μm)• Low metal content• Low endotoxin content

Description Quantity Cat. No. Ultrapure Water, qPCR Grade 1L BP2825-1

Ultrapure Water, qPCR Grade 10L BP2825-10

New

Page 20: Unrivaled Life Sciences - Fisher Scientific...Corning Spheroid Microplate, 384 Well, Black with Clear Round-Bottomed Wells, Ultra-Low Attachment (ULA) Coating, with Lid, Sterile 5/Case

19 Unrivaled Life Sciences fishersci.com/UnrivaledLifeSciences | fishersci.ca/UnrivaledLifeSciences

Fisher BioReagents 70% and 96% Ethanol SolutionsFisher BioReagents Molecular Biology Grade 70% and 96% Ethanol Solutions are ideal for concentrating nucleic acid samples by precipitation, and they work well in the presence of monovalent sodium, ammonium or other cations. These alcohol solutions are specifically tested for DNase, RNase and protease to verify the absence of hydrolytic enzymes that can degrade target biomolecules during nucleic acid and protein isolation and purification. Use them in combination with commercial mini-prep kits or for traditional extractions with organic solvents.

Features

• Reliable, ready-to-use alcohol solutions — no need to make your own

• Tested for DNase, RNase and protease • All federal taxes paid• Free of particulates (0.2μm filtered)• Multiple product packaging options to meet your specific needs

Laboratory Markets

• Academic labs (cell and molecular biology)• Biotech industry• Pharmaceutical industry• Government research labs

Test 70% Ethanol Solution 96% Ethanol SolutionAppearance Clear, Colorless Liquid Clear, Colorless Liquid

Color (APHA) <10 <10

Residue After Evaporation <0.001% <0.001%

Substances Darkened by Sulfuric Acid Pass Test Pass Test

Substances Reducing Permanganate Pass Test Pass Test

Assay (GC-TCD) Ethanol Water Methanol IPA

68 – 72% 28 – 32% NA NA

94 – 98% 2 – 6% NA NA

DNase Not Detected Not Detected

RNase Not Detected Not Detected

Protease Not Detected Not Detected

Product Description Quantity Cat. No.70% Ethanol Solution, Molecular Biology Grade 500mL BP8201-500

70% Ethanol Solution, Molecular Biology Grade 1L BP8201-1

70% Ethanol Solution, Molecular Biology Grade 4L BP8201-4

96% Ethanol Solution, Molecular Biology Grade* 500mL BP8202-500

96% Ethanol Solution, Molecular Biology Grade* 1L BP8202-1

96% Ethanol Solution, Molecular Biology Grade* 4L BP8202-4

*Only available in the U.S.

Page 21: Unrivaled Life Sciences - Fisher Scientific...Corning Spheroid Microplate, 384 Well, Black with Clear Round-Bottomed Wells, Ultra-Low Attachment (ULA) Coating, with Lid, Sterile 5/Case

20

Molecular Biology

fishersci.com/LifeSciences | fishersci.ca/LifeSciences

Invitrogen Qubit 4 FluorometerThe Qubit 4 Fluorometer is the latest edition of the popular Qubit fluorometer. It accurately measures DNA, RNA and protein quantity, and now it can measure RNA integrity and quality as well. The Qubit 4 Fluorometer and RNA IQ Assay Kit work together to accurately distinguish intact from degraded RNA in just two easy steps.

Features

• Convenient — requires as little as 1µL of sample• Easy to use — simply add your RNA sample to the RNA IQ working solution and then

measure• Fast — RNA degradation measured in under five seconds per sample

Visit fishersci.com/Qubit4Starter or fishersci.ca/Qubit4Starter to learn more.

New

Description Cat. No.Qubit Fluorometer and Starter Kits

Qubit 4 Fluorometer Q33226

Qubit 4 Quantitation Starter Kit Q33227

Qubit 4 NGS Starter Kit Q33228

Qubit 4 RNA IQ Starter Kit Q33229

RNA Assay Kits

Qubit RNA IQ (Integrity and Quality) Assay Kit, 75 Assays Q33221

Qubit RNA XR (Extended Range) Assay Kit, 100 Assays Q33223

Quant-iT RNA XR Assay Kit, 1,000 Assays Q33225

DNA Assay Kits

Qubit 1X dsDNA HS High-Sensitivity Assay Kit, 100 Assays Q33230

Quant-iT 1X dsDNA High-Sensitivity Assay Kit, 1,000 Assays Q33232

Visit thermofisher.com/qubit for a full list of Qubit Assays.

Page 22: Unrivaled Life Sciences - Fisher Scientific...Corning Spheroid Microplate, 384 Well, Black with Clear Round-Bottomed Wells, Ultra-Low Attachment (ULA) Coating, with Lid, Sterile 5/Case

21 Unrivaled Life Sciences fishersci.com/UnrivaledLifeSciences | fishersci.ca/UnrivaledLifeSciences

Invitrogen E-Gel Power Snap Electrophoresis SystemThe E-Gel Power Snap Electrophoresis System combines the devices and supplies that you need to start performing DNA electrophoresis and imaging — all in a small, lightweight benchtop system.

Features

• Faster analysis — go from sample loading to image capture in as little as 15 minutes • Simple operation — large touchscreen display and intuitive operating system• Safer and smarter — Power Snap and E-Gel precast gel cassettes eliminate the

need to handle hazardous chemicals

Product Description Quantity Cat. No. E-Gel Power Snap Electrophoresis Device 1 Device G8100

E-Gel Power Snap Electrophoresis System 1 System G8300

E-Gel Power Snap Electrophoresis System Starter Kit, EX 1% Includes: 10 E-Gel EX Agarose Gels (1%), E-Gel Power Snap Electrophoresis System, E-Gel 1kb Plus Express DNA Ladder with E-Gel Sample Loading Buffer (1X), Gel Opening Knife

1 Kit G8341ST

E-Gel Power Snap Electrophoresis Device Starter Kit, CloneWell II 0.8% Includes: 10 E-Gel CloneWell II Agarose Gels with SYBR Safe (0.8%), E-Gel Power Snap Electrophoresis Device, E-Gel 1kb Plus Express DNA Ladder with E-Gel Sample Loading Buffer (1X)

1 Kit G8168ST

Visit fishersci.com/PowerSnap or fishersci.ca/PowerSnap to learn more about all available devices and kits.

Page 23: Unrivaled Life Sciences - Fisher Scientific...Corning Spheroid Microplate, 384 Well, Black with Clear Round-Bottomed Wells, Ultra-Low Attachment (ULA) Coating, with Lid, Sterile 5/Case

22

Molecular Biology

fishersci.com/LifeSciences | fishersci.ca/LifeSciences

Browse our Unrivaled Life Sciences web page to view thousands of products for these major applications:

- Cell Culture

- Protein Biology

- Molecular Biology

From there, you can also download this brochure to share and view money-saving promotions on a variety of products.

Visit fishersci.com/UnrivaledLifeSciences or fishersci.ca/UnrivaledLifeSciences to explore our products you use every day.

at Your FingertipsTechnical Resources

Page 24: Unrivaled Life Sciences - Fisher Scientific...Corning Spheroid Microplate, 384 Well, Black with Clear Round-Bottomed Wells, Ultra-Low Attachment (ULA) Coating, with Lid, Sterile 5/Case

17-073-3003 KP 12/17 BN20172310

© 2018 Thermo Fisher Scientific Inc. All rights reserved. Trademarks used are owned as indicated at fishersci.com/trademarks.

In the United States:For customer service, call 1-800-766-7000To fax an order, use 1-800-926-1166To order online: fishersci.com

In Canada:For customer service, call 1-800-234-7437To fax an order, use 1-800-463-2996To order online: fishersci.ca

Unrivaled

Life SciencesUnrivaled

E S S E N T I A L S

Life Sciences