Ultraviolet/Visible (UV-Vis) Ultraviolet/Visible (UV-Vis) Spectroscopy of Potassium Spectroscopy of Potassium

PermanganatePermanganateBy Robert BohmanBy Robert Bohman

November 4November 4thth 2006 2006

CH EN 4903CH EN 4903

Importance to industryImportance to industry

• Potassium Permanganate is used to kill bacteria in reclaimed water

• Use UV-Vis to ensure that the concentration of Potassium Permanganate is at acceptable limit

OverviewOverview

• Theory

• Light Absorption Spectrum

• Experimental Procedure

• Results

• Conclusion

• Q & A

THEORYTHEORY

Properties of LightProperties of Light11

• c = λν c = speed of light in vacuum (2.998 x 108 m/s)

λ = wavelength (m)

v = frequency (Hz) • E = hc/ λ = hcv`

h = Planck’s constant (6.626 x 10-34 J•s)

v` = wavenumber (m-1)

Understanding Beer’s LawUnderstanding Beer’s Law22

• Transmittance T = P/P0

Schematic of Single-Beam Spectrophotometer, P0 is the irradiance entering sample, P is the irradiance leaving sample, and b is pathlength2

P = irradiance (energy per unit area of light beam)

Understanding Beer’s LawUnderstanding Beer’s Law33

• Absorbance A = log (P/P0) = -log (T)

• Beer’s Law A = εbc

ε = molar absorptivity (M-1 cm-1)

b = pathlength (cm)

c = concentration (M)

LIGHT ABSORPTION LIGHT ABSORPTION SPECTRUMSPECTRUM

Absorption Spectrum of LightAbsorption Spectrum of Light44

Wavelength of maximum absorption (nm)

Color Absorbed Color Observed

380 – 420 Violet Green-Yellow

420 - 440 Violet-Blue Yellow

440 – 470 Blue Orange

470 – 500 Blue-Green Red

500 – 520 Green Purple

520 – 550 Yellow-Green Violet

550 – 580 Yellow Violet-Blue

580 – 620 Orange Blue

620 – 680 Red Blue-Green

680 - 780 Purple Green

EXPERIMENTAL EXPERIMENTAL PROCEDUREPROCEDURE

Detecting Potassium Detecting Potassium PermanganatePermanganate

• Potassium permanganate (KMn04) in solution is purple / violet color meaning maximum absorption should be at 500 – 550 nm

• Prepared 5 known concentrations of KMnO4: 1ppm, 20ppm, 40ppm, 60ppm, 80ppm

Detecting Potassium Detecting Potassium PermanganatePermanganate

• Calibration Standards measured first on a Perkins-Elmer Lambda 35 over entire UV-Vis region to determine max absorption

• KMnO4 absorbed best at ≈ 520 nm

• A Bausch & Lomb Spectronic 21 was used to make all measurements

RESULTSRESULTS

UV-Vis Absorbance Readings for UV-Vis Absorbance Readings for Potassium Permanganate at 520 nmPotassium Permanganate at 520 nm

Average %A (after 3 runs)

Standard Deviation (%A)

1 ppm 0.015 0.004

20 ppm 0.256 0.001

40 ppm 0.520 0.004

60 ppm 0.753 0.002

80 ppm 1.046 0.001

Unknown #4 0.462 0.001

Calibration Curve for KMnOCalibration Curve for KMnO44 using UV-Vis using UV-Vis

Spectroscopy, Absorption vs. ConcentrationSpectroscopy, Absorption vs. Concentration

y = 0.0129x

R2 = 0.9990

0.2

0.4

0.6

0.8

1

1.2

0 20 40 60 80 100

Concentration (ppm)

%A

bso

rban

ce

Determination of Unknown Determination of Unknown Concentration of KMnOConcentration of KMnO44

• Used cuvette of 1cm length

• ε = slope of line = 0.029 ppm-1 cm-1

• Unknown #4 concentration found using

c = A/0.029

• 36 ppm = 0.462 %A / 0.029 ppm-1

Error AnalysisError Analysis

• Used 10.00 ± 0.05mL volumetric pipette to make all solutions

• Measured density of water with:

= (999.8392 + 16.945176t – 7.9870401*10-3t2 – 46.170461*10-6t3 + 105.56302*10-9t4 – 280.54253*10-12t5)/(1 + 16.879850*10-3t)5

H2O = 0.997883 g/mL at 21.5°C

• Measured accuracy of scale to be 0.0005g

Error in UnknownError in Unknown

• Errors determined graphically from calibration curve

• A = ± 0.01%

• Concentration = ± 1.00 ppm

• Final concentration of Unknown #4 was

36 ± 1.00 ppm

CONCLUSIONCONCLUSION

ConclusionConclusion

• How accurate are results?

Can be determined by R2 value for slope of calibration curve.

For this example R2 = 0.999

ConclusionConclusion

• Use Beer’s law to determine concentration of unknown concentration

• Find the molar absorptivity through the slope of calibration curve

• Determined ε = 0.029 ppm-1 cm-1

• Determined Unknown #4 concentration to be 36 ± 1.00 ppm

Q & AQ & A

Special ThanksSpecial Thanks

To Triston Thorpe and Jason To Triston Thorpe and Jason Judkins in helping with the Judkins in helping with the

experimentexperiment

ReferencesReferences

1. Harris, Daniel C. Sixth Edition Quantitative Chemical Analysis. Pg. 408-409. New York: W.H. Freeman and Company, 2003.

2. Harris, Daniel C. Sixth Edition Quantitative Chemical Analysis. Pg. 410. New York: W.H. Freeman and Company, 2003.

3. Harris, Daniel C. Sixth Edition Quantitative Chemical Analysis. Pg. 411-412. New York: W.H. Freeman and Company, 2003.

4. Harris, Daniel C. Sixth Edition Quantitative Chemical Analysis. Pg. 413. New York: W.H. Freeman and Company, 2003.

5. CRC Handbook of Chemistry and Physics. Pg. F-6. Cleveland, Ohio: The Chemical Rubber Co., 1968.

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