Tumor-Infiltrating Immune Cell Marker Guide for IHC (Mouse ......Th1 T-Bet Th2 GATA-3+ Th17 RORγt+...
Transcript of Tumor-Infiltrating Immune Cell Marker Guide for IHC (Mouse ......Th1 T-Bet Th2 GATA-3+ Th17 RORγt+...
18-IM
M-1
8284
-FLY
2-E1
For R
esea
rch
Use
Only
. Not
For
Use
In D
iagn
ostic
Pro
cedu
res.
For pathway key and background, please visit:
Tumor-Infiltrating Immune Cell Marker Guide for IHC (Mouse)Tumor-Infiltrating Immune Cell Marker Guide (Mouse)
rev. 11/28/18
Granulocytes Monocytes
CD14+andF4/80–
Plasma Cells
BCMA+orCD138+
Macrophages
F4/80 high
M1-likeCD86+or CD80+or iNOS+
M2-likeCD163+orCD206+or Arginase+
B Cells
CD19+
Naïve
IgD+andCD27–
SwitchedMemory
IgD–andCD27+
Unswitched
IgD+andCD27+
PlasmacytoidDendritic Cells
Siglec-H+andCD317+
Activated
CD83+NeutrophilsF4/80 med andLy-6G+
EosinophilsCD193+andF4/80 med andSiglec-F+
BasophilsFcεR1α+andCD117–
Mast cellsFcεR1α+andCD117+andCD23
Cel
l Typ
eC
lass
NKT Cells
CD3+andNK1.1+
Type I NKT (iNKT) TCR Vα14-Jα18+
Type II NKT
Th1T-Bet+
Th2GATA-3+
Th17RORγt+
TregFoxP3+andCD25+
T Cells
CD3+
HelperT Cells (Th)CD4+
CytotoxicT CellsCD8+
Activated
CD69+orCD25+
Naïve
CD44–andCD62L+
Eector Memory
IL7Rα+andCD44+and CD62L–
Cytotoxic
Granzyme+
or Perforin+
Exhausted
PD-1+and TIM-3+
and LAG3+
Eector
CD44+andCD62L–
Central Memory
IL7Rα+andCD44+and CD62L+
NK Cells
NK1.1+orNKp46+orNKG2D+andCD3–
CD69+
Activated
Cytotoxic
Granzyme+
or Perforin+
CD11b+
DCsCD11b+and CD8α–andCD103–orXCR1–or CLEC9A–
CD8α+
ResidentDCsCD8α+
CD103+
MigratoryDCsCD103+
Conventional Dendritic Cells
CD11c+andMHCII+
Activated
CD83+
XCR1/CLEC9A+ DCs (excel at cross-presentation)XCR1+or CLEC9A+
Sub
typ
es
Monocytic (M) MDSCsLy6C+andLy6G–and Arginase+
Polymorphonuclear (PMN) MDSCs Ly6C lowandLy6G+and Arginase+
Myeloid-Derived Supressor Cells (MDSCs)
Myeloid Cells
CD11b+
Lymphoid Cells
CD45+
Leukocytes
CD45+
+ positive /medium/high expression– negative/ low expression
Functional State Markers
Key
cst-science.com/mousemarkers
18-IM
M-1
8284
-FLY
2-E1
For R
esea
rch
Use
Only
. Not
For
Use
In D
iagn
ostic
Pro
cedu
res.
APPLICATIONS & REACTIVITY KEY: WB Western Blotting / IP Immunoprecipitation / IHC Immunohistochemistry / IF Immunofluorescence / F Flow Cytometry / ChIP Chromatin Immunoprecipitation / -IC Immunocytochemistry / -P Paraffin / -F Frozen /E-P Peptide ELISA / H human / M mouse / R rat / Hm hamster Mk monkey / C chicken / Mi mink / Dm D. melanogaster / X Xenopus / Z zebrafish / B bovine / Dg dog / Pg pig / Sc S. cerevisiae / All all species expected / ( ) 100% sequence homology
For pathway key and background, please visit:
© 2019 Cell Signaling Technology, Inc. Cell Signaling Technology, CST, and XP are trademarks of Cell Signaling Technology, Inc. U.S. Patent No. 5,675,063. All other trademarks are the property of their respective owners.
Key Products for Mouse Tumor-Infiltrating Immune Cell Markers for IHCVisit www.cellsignal.com for full listing of antibodies, related kits, and reagents.
cst-science.com/mousemarkers
ANTIBODIES APPLICATIONS REACTIVITY
#93668 Arginase-1 (D4E3M™) XP® Rabbit mAb WB, IHC-P, IF-F, IF-IC, F H, M, R
#99940 CD3ε (D4V8L) Rabbit mAb WB, IHC-P M
#25229 CD4 (D7D2Z) Rabbit mAb WB, IHC-P M, R
#98941 CD8α (D4W2Z) XP® Rabbit mAb (Mouse Specific)
WB, IHC-P M
#97585 CD11c (D1V9Y) Rabbit mAb WB, IHC-P, IF-F, IF-IC M
#90176 CD19 (D4V4B) XP® Rabbit mAb WB, IP, IHC-P H, M, (B)
#37259 CD44 (E7K2Y) XP® Rabbit mAb WB, IHC-P H, M, R
#70257 CD45 (D3F8Q) Rabbit mAb IP, IHC-P, IF-F, IF-IC M
#70076 F4/80 (D2S9R) XP® Rabbit mAb WB, IP, IHC-P M
#12653 FoxP3 (D6O8R) Rabbit mAb IHC-P, IF-F, F M
#5852 GATA-3 (D13C9) XP® Rabbit mAb WB, IHC-P, IF-IC, F, ChIP H, M, (Mk)
#46890 Granzyme B (D6E9W) Rabbit mAb WB, IHC-P H, M
#84651 PD-1 (D7D5W) XP® Rabbit mAb (Mouse Specific)
WB, IP, IHC-P, IF-F, IF-IC, F
M, (R, Hm)
#4744 SSEA1 (MC480) Mouse mAb IHC-P, IF-IC, F M
#83882 TIM-3 (D3M9R) XP® Rabbit mAb Mouse Specific)
WB, IP, IHC-P M
CD3ε (D4V8L) Rabbit mAb #99940: IHC analysis of paraffin-embedded mouse small intestine using #99940 performed on the Leica® BOND™ Rx.
CD4 (D7D2Z) Rabbit mAb #25229: IHC analy-sis of paraffin-embedded 4T1 metastatic tumor in mouse lung using #25229 performed on the Leica® BOND™ Rx.
CD19 (D4V4B) XP® Rabbit mAb #90176: IHC analysis of paraffin-embedded mouse spleen using #90176.
F4/80 (D2S9R) XP® Rabbit mAb #70076: IHC analysis of paraffin-embedded mouse small intestine using #70076.
CD8α (D4W2Z) XP® Rabbit mAb (Mouse Specific) #98941: IHC analysis of paraffin-embedded mouse spleen using #98941.
Granzyme B (D6E9W) Rabbit mAb #46890: IHC analysis of paraffin-embedded mouse 4T1 mammary tumor using #46890.
FoxP3 (D6O8R) Rabbit mAb #12653: IHC analysis of paraffin-embedded mouse spleen using #12653.
PD-1 (D7D5W) XP® Rabbit mAb (Mouse Specific) #84651: IHC analysis of paraffin-embedded mouse LL/2 syngeneic tumor using #84651 performed on the Leica® Bond™ Rx.