trypanosome infection
Transcript of trypanosome infection
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Trypanosome infections in cattle usually result in a chronic disease, characterized by anaemia,
leukocytopaenia, immunosuppression and cachexia. The parasites live in the bloodstream andevade immunological control by continuously varying their surface glycoprotein coat. Antibody
to the surface-exposed epitopes of the variable surface glycoprotein coat (VSG) mediates the
removal of a particular variant, but new variants expressing antigenically different VSGs appear
throughout the infection. In cattle, following tsetse-fly transmitted infections, a fluctuatingparasitaemia is observed, with-several variants expressed at any one time. Some breeds of cattle
such as the N'Dama (Bos taurus) have evolved the ability to control both parasitaemia and
anaemia. The control of these two traits does not appear to be linked. Trypanotolerant cattleremain more productive in trypanosomiasis-endemic areas thanB. indicus breeds (such as the
Boran), but can succumb to the disease when exposed to very high challenge or stress.
ILRAD's immunopathology program aims to elucidate the mechanisms of trypano-tolerance and
to identify trypanosome antigens which cause either protective or pathological responses in
infected cattle. These antigens will be used in the design of novel vaccines which elicit protectiveanti-parasite immune responses or to block pathogenic responses.
Results from studies on immune responses following T. congolense infections have shown thatN'Dama cattle produce higher levels of IgG1 antibodies to invariant antigens (specifically, a
trypanosome cysteine protease and a heat shock protein) and variant antigens; they have earlier
and higher T-cell proliferative responses and their monocytes secrete higher levels ofcostimulatory cytokines (IL1/IL6) early in infection compared to trypanosusceptible cattle. A
population of IgM antibodies which bind to non-trypanosome antigens has been identified in
Borans but not in N'Damas. This population of antibodies also binds to trypanosome VSG,
suggesting that they are polyspecific in origin. Studies are currently under way into differentialepitope recognition and antibody avidity between the two breeds. There appear to be no
differences between breeds in the titre or isotype of antibody specific for the surface-exposed
epitopes of VSG. Both N'Dama and Boran cattle experience a profound macrophage-mediatedimmunosuppression in the lymph nodes early in infection. The antigen which induces this
suppressor activity has been purified and is being characterized. Also a massive increase in the
numbers of CD5+
B cells and in serum IgM levels has been described in all infected cattle. It has
been suggested that the CD5+
B-cell response is the result of a T-independent response to VSG.
The primary pathological feature of bovine trypanosomiasis is anaemia. The drop in red cells isdue to the removal of both mature and immature erythrocytes by cells of the mononuclear
phagocytic system (MPS) in the spleen and bone marrow. Results from in vivo and in vitroexperiments suggest that erythrophagocytosis is higher in Borans than N'Damas. Data have also
been obtained which indicate that in addition to the massive removal of erythrocytes by the MPS,there is insufficient red blood ceil replacement into the circulation. Analysis of erythroid
progenitor colonies in bone marrow from trypanosusceptible cattle has shown that there is a
suppression of both early (BFU-E) and late (CFU-E) progenitor cells early in infection. As the
infection progresses the CFU-E progenitors recover, but the numbers of BFU-E's remaindepressed.
In summary, we have accumulated a database in which differences in the pathologicalconsequences of trypanosome infection have been quantified in trypanotolerant and
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trypanosusceptible cattle. Other factors which affect the outcome of disease are being addressed,
such as the role of specific cytokines or the direct pathogenic effects on host molecules, cells orprocesses, of certain trypanosome antigens. Can we use the data we have to develop models to
test the effect of altering variables such as aberrant cytokine production or the failure to develop
T-dependent antibody responses? Can we test how these different immunological and
haematological processes interact to affect the outcome of disease? Is it possible to model theinteractions between innate host factors versus specific immune responses in control of parasite
growth and the role of the immune and erythroid responses in the control of pathogenesis?