Truncated Protein on SDS

7/30/2019 Truncated Protein on SDS

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Truncated Protein on SDS-PAGE_________

#608763sarim - 08/22/11 21:34

http://www.iovs.org/content/41/9/2466/F1.large.jpg

ust wanted to share this.

e.g. due to the "Non -sense Mutation(STOP Codon)", note that "Truncated Protein" Band appear at theBottom on SDS-PAGE due to it's smaller size.

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* Re:Truncated Protein on SDS-PAGE_________

#2455460technodoctor - 08/22/11 21:43

thankx :)Report Abuse


#2455463sharif0812 - 08/22/11 21:45

hey thanks sarim for this---do u kno why the truncated one is on the bottom??Report Abuse


#2455470technodoctor - 08/22/11 21:58

@sharif0812....the truncated one is smaller in size and therefore would travel farther. The normalprotein is heavier and therefore remains closer to original position. I hope you know that theseproteins travel towards the opposite charge in electrical feild...they being -vely charged travel towards+ electrode... the lighter the protein, the farther it will go

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#2455591step1saga - 08/23/11 02:51

Thanks sarim!!

I think you should get be the only person to get a 300 ...and I m sure you'll come pretty darn close :)(also saying this based on your nbme explanations--thanks again)

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#2455593step1saga - 08/23/11 03:00

300/99*

Im gonna add something about sickle cell on sds page

HbA = Is valine( which is a hydrophobic AA)-- so it goes towards the positive charge in the electrodeeHbS = neutralHbC= is lysine (positively charged AA)- moves towards the negative electrode

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#2455595step1saga - 08/23/11 03:03

does SDS page involve charged electrodes? or just argose gel?Report Abuse

* Re:Truncated Protein on SDS-PAGE_________ #2455598sarim - 08/23/11 04:03

@step1saga thanks for ur nice words.wish u all the best.

SDS-PAGE:-is an Electrophoresis as the name states(SDS-polyacrylamide gel Electrophoresis), so it does involve+ and - poles.......BUT separate the protein strands by their "SIZE" (and not by their Intrinsic Charge)
http://www.iovs.org/content/41/9/2466/F1.large.jpghttp://reportmessage%28608763%29/http://reportmessage%28608763%29/http://reportreply%282455460%29/http://reportreply%282455460%29/http://reportreply%282455463%29/http://reportreply%282455463%29/http://reportreply%282455470%29/http://reportreply%282455470%29/http://reportreply%282455591%29/http://reportreply%282455591%29/http://reportreply%282455593%29/http://reportreply%282455593%29/http://reportreply%282455595%29/http://reportreply%282455595%29/http://reportreply%282455595%29/http://reportreply%282455593%29/http://reportreply%282455591%29/http://reportreply%282455470%29/http://reportreply%282455463%29/http://reportreply%282455460%29/http://reportmessage%28608763%29/http://www.iovs.org/content/41/9/2466/F1.large.jpg

7/30/2019 Truncated Protein on SDS

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-the gel is prepared with wide range of average Pore sizes

-u can read about SDS-PAGE on net, it will help u solve Qs that otherwise seem weird.-Here are some Diagrams in the following links ......if u would like further discussion, i'll be glad to join.

https://reader009.{domain}/reader009/html5/0424/5ade71209c998/5ade7125c9daa.jpg

http://ww2.chemistry.gatech.edu/~lw26/bCourse_Information/4581/techniques/gel...ect/gel.jpg

http://talon.niagara.edu/~391s08/giacomini/images/sds_page.png

https://reader009.{domain}/reader009/html5/0424/5ade71209c998/5ade71264201e.png

Note that the standard molecular wt (on the Lt side) are given as reference and they are spaced outfrom Top(larger fragments) towards Bottom(Smaller Fragments)

https://reader009.{domain}/reader009/html5/0424/5ade71209c998/5ade712672eb2.jpg

good luck.

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#2456028odin - 08/23/11 19:49

Thanks, sarim! Very helpful.Report Abuse


#2456050step1saga - 08/23/11 20:52

thanks for the links sarim :)Report Abuse


#2456051sarim - 08/23/11 21:09

:) thanks

Note how SDS give out Negative charge to all protein molecules (Masking the protein Intrinsic charge)https://reader009.{domain}/reader009/html5/0424/5ade71209c998/5ade71286a4e3.jpg

http://www.gibthai.com/userfiles/image/technote/Western%20Blot/Western...-Blot-2.jpg

*they can also put it sidewayshttp://www2.montana.edu/cftr/images/sds_page5.gif

step1saga - 08/23/11 21:19

yeah that makes charge to be completely inconsiderable or negligible to have any significant effect sothey would definitely not show sickle cell anemia on a SDS. Since defiinitely determined by size.

I almost turned my laptop 90 degrees to see the one sideways..Report Abuse


#2456109psychmledr - 08/23/11 22:57

Thank you so much :)
http://www.molecularstation.com/images/protein-gel-electrophoresis.jpghttp://ww2.chemistry.gatech.edu/~lw26/bCourse_Information/4581/techniques/gel_elect/gel.jpghttp://talon.niagara.edu/~391s08/giacomini/images/sds_page.pnghttp://biochem.co/wp-content/uploads/2008/08/sds-page-gel-and-graph.pnghttp://www.personal.psu.edu/jms5704/blogs/simmons/1476-7961-4-12-1.jpghttp://reportreply%282455598%29/http://reportreply%282455598%29/http://reportreply%282456028%29/http://reportreply%282456028%29/http://reportreply%282456050%29/http://reportreply%282456050%29/http://www.ebioe.com/uploadfile/2010/0409/20100409022030595.jpghttp://www.gibthai.com/userfiles/image/technote/Western%20Blot/Western-Blot-2.jpghttp://www2.montana.edu/cftr/images/sds_page5.gifhttp://reportreply%282456055%29/http://reportreply%282456055%29/http://reportreply%282456055%29/http://www2.montana.edu/cftr/images/sds_page5.gifhttp://www.gibthai.com/userfiles/image/technote/Western%20Blot/Western-Blot-2.jpghttp://www.ebioe.com/uploadfile/2010/0409/20100409022030595.jpghttp://reportreply%282456050%29/http://reportreply%282456028%29/http://reportreply%282455598%29/http://www.personal.psu.edu/jms5704/blogs/simmons/1476-7961-4-12-1.jpghttp://biochem.co/wp-content/uploads/2008/08/sds-page-gel-and-graph.pnghttp://talon.niagara.edu/~391s08/giacomini/images/sds_page.pnghttp://ww2.chemistry.gatech.edu/~lw26/bCourse_Information/4581/techniques/gel_elect/gel.jpghttp://www.molecularstation.com/images/protein-gel-electrophoresis.jpg

Truncated Protein on SDS

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