8/10/2019 Training curriculum and Registration form - BIOT.doc

http://slidepdf.com/reader/full/training-curriculum-and-registration-form-biotdoc 1/2

Training Curriculum

Techniques in Molecular Biology

Theory and practical in

Macro molecules – DNA, RNA and protein - their properties and function – isolation from plants

and microbes. Restriction digestion. Spectrophotometry Agarose and Polyacrylamide gel

electrophoresis for nucleic acids. Protein purification. SDS page. lotting techni!ues. "estern

lot. Southern lot. Recombinant DNA technology – cloning #ectors and cloning. $igation,

transformation and recombinant selection. Polymerase chain reaction. Molecular mar%ers and

genome mapping. Analysis of data using soft&ares. Se!uence analysis using ioinformatics

tools. 'M technology and biosafety regulations.

Plant Tissue Culture and Applications

  Theory and practical in

Plant tissue culture – principles and concepts – techni!ues in P() – essential re!uirements – 

different media used – culture conditions – different routes – organogenesis, embryogenesis – 

direct and indirect methods. Different stages – establishment, multiplication, proliferation, rooting

and hardening. Applications – mass multiplication, crop impro#ement, conser#ation, secondarymetabolite production, transgenics. *alidation methods – clonal fidelity, genetic stability. (rouble

shooting – concerns and issues. )ost benefit analaysis.

Hands on Training in techniques in Molecular Biology and Biotechnology

+ands on eperience &ill be pro#ided to #arious techni!ues li%e plant tissue culture, isolation of 

nucleic acids, P)R techni!ues, agarose gel electrophoresis and gel documentation, primer 

designing, purification of P)R products, preparation of competent cells, cloning, restriction

analysis, se!uencing and se!uence analysis, molecular mar%ers RAPD, SSR, SSR/, genetic

engineering, blotting and hybridi0ation, molecular diagnostics, protein etraction and analysis by

PA'1 and SDS page, chromatography techni!ues, 1$SA en0yme lin%ed immune sorbent

assay/, production of biofertilisers etc

8/10/2019 Training curriculum and Registration form - BIOT.doc

http://slidepdf.com/reader/full/training-curriculum-and-registration-form-biotdoc 2/2

REGISTRATI! "RMCE!TRE "R BITECH!#G$ A!% M#EC&#AR BI#G$ 'CPBMB()

IT*BT CMP#E+) ,ERA#A AGRIC&#T&RA# &!I-ERSIT$

-E##A!I,,ARA .P/0) THRISS&R 1 234 252

Tel6 4738*97:35881-mail2 cpbmb%au3gmail.com, %aubioinfo3gmail.com, cpbmb3%au.in4. Name 2

5. Designation 2

6. Speciali0ation 2

7. Post graduation 8)ompleted9 pursuing: 2

;. nstitute9)ollege9<ni#ersity 2

=. (elephone No. 2

>. Address for )orrespondence 2

?. 1-mail Address 2

@. (raining programme applied for 2

Preference

  4. (echni!ues in Molecular iology ..

  5. Plant tissue culture B Applications ..

 6. +ands on training on (echni!ues in Molecular iology and iotechnology .......

 

Mar% a tic% √/ legibly. Applicants can apply for more than one programme in a single registration form. n that

case preference may be indicated in Roman numeral against the Sl. No. of the programme. (he follo&ing

information may please be gi#en2-

4C. Mode of payment2 i/ n cash at )entral (raining nstitute or ii/ As DD in fa#our of Professor of 

1tension, )(, Mannuthy payable at S(, llu%%ara and to be sent to2 Professor B +ead, )PM,

(-( )omple, Eerala Agricultural <ni#ersity, *ellani%%ara, 8P.:, (hrissur – =?C =;=

DD no.... Dated.. Amount..

44. Four in#ol#ement in Plant iotechnology B Molecular iology2

45. ProGect title f applicable/ 2

46. Details of similar training9&or%shop attended 2

47. Hustification for considering for the training Programme selected 2

 

Place2

Date2 Signature