R-Biopharm AG

Tracking down hidden allergensbioavid Lateral Flow Rapid Tests for allergen management

Applications to produce safer food:

• hygiene swabbing tests• rinse water analysis• food product control

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R-Biopharm – for the safety of your analysis.

Milk

Peanut

Egg

Mustard

Hazelnut

Almond

Pistachio

Cashew

Brazil Nut

Coconut

Macadamia Nut

Sesame

Crustacean

Soy

Walnut

Simple&

Rapid

Detect food allergens with less effort and greater reliability for allergen free products and safer food.

Allergen management in food companies is becoming increasingly important. Health risks due to contaminated food must be detected and prevented in time. The HACCP concept (Hazard Analysis and Critical Control Points) requires checking harmful residues in food. It is possible to carry out residue and hygiene controls by means of immunochemical tests. R-Biopharm offers lateral flow allergen tests in its product range for R-Biopharm allergen management systems. With these rapid tests it is possible to detect food allergens rapidly and reliably even while the production pro-cess is underway as per your HACCP plan. Cross contamination is detectable and

preventable. Allergen free products and safer food can be checked by using these tests to protect sensitive consumers from adverse reactions. The sensitivity of the specific lateral flow tests is comparable to that of commercial ELISAs (Enzyme-linked Immunosorbent Assay) for the matrices under investigation. Lateral flow allergen tests contain specific antibodies which are conjugated to tracers and which are delivered in a dried and stable format. After activation and run of the specific antigen antibody reaction colored lines appear on the test strip.

Test information

• Lateral Flow device• 3 pack sizes available (10/25/50 determinations)• analytical sensitivity: 1 - 5 mg/kg (ppm)• detection limit for swabbing tests: 10 µg of the allergen• visual yes/no results• qualitative result• result after 10 min plus max. 10 min sample preparation• no lab equipment required• on-site application• Kit reagents ready to use• high sensitivity and specificity• versatile application: hygiene swabbing test or rinse water

analysis (production accessories, machines, working place,warehouse environment, transportation, etc.),raw material and end-product control

The bioavid lateral flow allergen tests are tools for efficient detection of allergen contamination on production lines, and in food. In particular the ease of use and the speed of analysis make them valuable for the implementation and monitoring of HACCP concepts in the food industry.

no equipment required

reliable yes/no results

results in 10 min

Sample preparation

The swab sample or food extract is pipetted into the reaction vial which contains specific antibody ready to use. If the antigen is included in the sample the antigen antibody complex is formed.

The complex is detected on the test strip. The existence of 1 line indicates a valid negative result. The existence of 2 lines indicates a valid positive result.

Test procedure

Total assay time: 10 minutes

Allergen Detection / Test Kits

All reagents are provided with the kit, offer superior stability and storage atroom temperature

Add 0.2 ml or 7 dropsRunning Buffer into a Reaction Vial

Add 0.1 ml or 2 drops (with pasteur pipette) prepared sample into the Reaction Vial*Incubate for 5 min

Insert an Allergen Test Strip into the Reaction Vial

Read the result after 5 min

1 line indicates a valid negative result2 lines indicate a valid positive result

Sampling time: less than 2 min

Sample preparation Swab-Test (Kit)

Add 1 ml distilled water into a Sample Vial

Add 0.1 ml or 3 drops Buffer Concentrate

Insert a Swab into the Sample Vial and moisten it

Swab the area of interest

Insert the Swab into the Sample Vial again, mix and press to the side of the vial to release the material from the Swab

Further steps see test procedure

Sample Preparation Food Products (except chocolate)

For e.g. nuts, nut mixtures, bakery

Weigh 50 g of the sampleWeigh 4 g salt

Total time for sample preparation: less than 10 min

Pour into a mixer and add 450 ml dist. waterHomogenize for 5 min

Filter, centrifuge or allow homogenate to settle

Further steps see test procedure

* Exception: Milk, Hazelnut Kits (add 0.2 ml or 4 drops with pasteur pipette)

For detailed remarks see „Detailed Instructions“ under www.bioavid.com

bioavid Diagnostics – Lateral Flow Tests

Test format Lateral Flow test strips (10, 25, 50 determinations)

Detection limit (Sensitivity)

1 - 10 mg/kg (ppm) in food or 10 µg of the allergen on surfaces

Application Swabbing test, rinse water analysis, food product control

Interpretation Visual (qualitative result)

Total assay time 10 min

Lab equipment Not required (Kit contains ready to use reagents)

Ref. Product Description No. of Test / Amount Art. No.

Lateral Flow Tests

Coconut Qualitative detection of coconutDetection limit: 1 mg/kg (ppm)

10 or 25 Test stripsIncubation time: 10 min

BL600-10BL600-25

Almond Qualitative detection of almondDetection limit: 1 mg/kg (ppm)

10 or 25 Test stripsIncubation time: 10 min

BL601-10BL601-25

Brazil Nut Qualitative detection of brazil nutDetection limit: 1 mg/kg (ppm)

10 or 25 Test stripsIncubation time: 10 min

BL602-10BL602-25

Mustard Qualitative detection of mustardDetection limit: 1 mg/kg (ppm)

10 or 25 Test stripsIncubation time: 10 min

BL603-10BL603-25

Hazelnut Qualitative detection of hazelnutDetection limit: 5 mg/kg (ppm)

10 or 25 Test stripsIncubation time: 10 min

BL604-10BL604-25

Macadamia Nut Qualitative detection of macadamia nutDetection limit: 1 mg/kg (ppm)

10 or 25 Test stripsIncubation time: 10 min

BL605-10BL605-25

Peanut Qualitative detection of peanutDetection limit: 5 mg/kg (ppm)

10 or 25 Test stripsIncubation time: 10 min

BL606-10BL606-25

Walnut Qualitative detection of walnutDetection limit: 10 mg/kg (ppm)

10 or 25 Test stripsIncubation time: 15 min

BL607-10BL607-25

Egg Qualitative detection of eggDetection limit: 1 mg/kg (ppm)

10 or 25 Test stripsIncubation time: 10 min

BL608-10BL608-25

Sesame Qualitative detection of sesameDetection limit: 1 mg/kg (ppm)

10 or 25 Test stripsIncubation time: 10 min

BL609-10BL609-25

Cashew Kernel Qualitative detection of cashew kernelDetection limit: 1 mg/kg (ppm)

10 or 25 Test stripsIncubation time: 10 min

BL610-10BL610-25

Pistachio Qualitative detection of pistachioDetection limit: 1 mg/kg (ppm)

10 or 25 Test stripsIncubation time: 10 min

BL611-10BL611-25

Milk Qualitative detection of milkDetection limit: 1 mg/kg (ppm)

10 or 25 Test stripsIncubation time: 10 min

BL613-10BL613-25

Soy Qualitative detection of soyDetection limit: 1 mg/kg (ppm)

10 or 25 Test stripsIncubation time: 10 min

BL615-10BL615-25

Crustacean Qualitative detection of crustaceanDetection limit: 10 mg/kg (ppm)

10 or 25 Test stripsIncubation time: 10 min

BL616-10BL616-25

Combi Kit Free 10 x combinations of above-named Allergen tests

50 Test stripsIncubation time: 10 min

BLC6-50

Accessories

Swabbing Kit Kit for sampling of allergen residueson environmental surfaces(e.g. on food production lines)

25 Swabs, vials, pipettes, 10 ml buffer concentrate

BS800-25

Absorption Buffer Buffer for preparation of polyphenol containing samples (coffee, red wine)

25 Vials (9 ml buffer each)

BS810-25

Lab Service Sample validation, application recommendation

Processing timeapprox. 1 week

Specifi cations