TOWARDS SUSTAINABLE, WIRELESS, AUTONOMOUS … · -0.32-0.30-0.28 measurement sensor 1 measurement...
Transcript of TOWARDS SUSTAINABLE, WIRELESS, AUTONOMOUS … · -0.32-0.30-0.28 measurement sensor 1 measurement...
TOWARDS SUSTAINABLE, WIRELESS, AUTONOMOUSNANOCELLULOSE-BASED QUANTITATIVE DRUG OFABUSE BIOSENSING PLATFORM
Inkjet printing of GO-MB composite
The inkjet parameters of drop spacing, temperature, waveform and number of layers
have been tailored, and the final settings are shown in the table.
Graphene Oxide / Methylene Blue Ink (GO-MB)
For the ink formulation, we explored in detail the GO-MB interaction
mechanism based on π-π-stacking and ionic interactions as well as the
specific antibody attachment.
For preparation of antibody (AB) GO-MB inks, the native MB is partly
exchanged with antibodies conjugated with MB.
GO-MB ink formulation and characterization (no antibody)
Eva Melnik1// [email protected] // Christoph Steininger1, Steffen Kurzhals1, Giorgio Mutinati1
1 AIT Austrian Institute of Technology GmbH, Center for Health and Bioresources, Molecular Diagnostics, Giefinggasse 4, 1210 Vienna, Austria.
Parameters selected
Drop spacing 5
Plate temperature
40°C
Number of layers
2
Drug of Abuse Electrochemical Sensor
In the project GREENSENSE we merge molecular
detection and printed electronics in the form of a fully-
integrated biosensing platform using nanocellulose (NC).
The biosensing platform with the newly developed printed
(DoA) biosensors will integrate different NC-based printed
electronic components (supercapacitor and/or a primary
battery, display and NFC antenna) and a single microchip
to enable wireless communication and electrochemical
readout.
Assay: We target label-free electrochemical assays [1-3]
employing a redox-active layer system modified with
capture molecules. In this type of assay, specific binding
events reduce the electron transport through the layer
system to the electrode.
Our approach is the
development of an inkjet
printable composite
based on graphene oxide
(GO) modified with
antibody-methylene blue
conjugates (MB-AB).
[1] Li, Yan; et al. (2011) Anal. Sci. 27(7), 727.
[2] Du, Yan; et al. (2010) Biosens. & Bioelect. 25(8), 1902.
[3] Kong, Fen-Ying; et al. (2013) Chem. Comm. 49(11),1052.
Drug of Abuse Detection
Commercially available detection devices:
The drug of drug of abuse (DoA) is highly interesting for many fields of
applications e.g. employment setting (safety-sensitive positions in transport,
construction or sports) or the criminal justice system. The existing devices use
either urine or saliva as sample liquid and employ lateral flow based tests
systems. The appearance of a colored line indicates if a certain drug (e.g.
cocaine, morphine, tetrahydrocannabinol, amphetamine, secobarbital) was
consumed.
New challenges:
For the improvement of the existing systems, the project Greensense aims at:
• quantitative electrochemical detection of drugs of abuse in urine and saliva
• measurement time <10 min
• detection in the ng/mL range
This project has received funding from the European Unions Horizon 2020 research and innoviation
programme under grant agreement No 761000. This publication only reflects the authors views and the
European Union is not liable for any use that may be made of the information contained therein.
HTTP://GREENSENSE-PROJECT.EU/
0,0 0,5 1,0 1,5 2,0 2,5 3,0 3,5
0,0
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1,2
Peak c
urr
ent
diffe
rence (D
I p) [µ
A/m
m2]
anti-IgG HRP (L) used for immobilization [µM]
ΔIP = ΔIP(𝑚𝑎𝑥)[𝐿]
𝐾𝑑+[𝐿]
2.) activity
and sensing
properties
1.) GO-MB
ink formulation and
characterization
Effect of NaCl concentration Effect of pH values (in 50mM
NaCL)
Effect of glucose (in 50mM
NaCL)
-0.5 -0.4 -0.3 -0.2 -0.1 0.0
0
200
400
600
800
1000
1200
I no
rma
lize
d [nA
/mm
2]
Potential [V]
0mM 1min
0mM 10min
5mM 1min
5mM 10min
9mM 1min
9mM 10min
13mM 1min
13mM 10min
17mM 1min
17mM 10min
20mM 1min
20mM 10min
Differential Pulse Voltammetry (DPV) of MB-GO ink (no antibody)
Inkjet printed MB-GO on
working electrode of screen
printed three-electrode sensor
Differential pulse voltammetry
(scan rate:0.5V/s, step: 0.005V,
Epuls: 0.05V, tpuls=0.005s)
AgCl reference electrode
Carbon paste counter electrode
Carbon paste working electrode
-0.5 -0.4 -0.3 -0.2 -0.1 0.0
0
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1000
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1400
1600
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2000 pH5
pH6
pH7
pH8
pH9
I no
rma
lize
d [nA
/mm
2]
Potential [V]
0 2 4 6 8 10
800
850
900
950
1000
1050
1100
1150
1200
pH 5
pH 6
pH 7
pH 8
I norm
aliz
ed (
nA
/mm
2)
Time [min]
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0
200
400
600
800
1000
1200
1400
1600
I no
rmaliz
ed [
nA
/mm
2]
Potential [V]
10mM NaCl
31mM NaCl
51mM NaCl
69mM NaCl
85mM NaCl
100mM NaCl
114mM NaCl
127mM NaCl
0 20 40 60 80 100 120 140 160 180
-0.42
-0.40
-0.38
-0.36
-0.34
-0.32
-0.30
-0.28
measurement sensor 1
measurement sensor 2
measurement sensor 3
measurement sensor 4
Pote
ntial [V
]
Conc. NaCl [mM]
0 20 40 60 80 100 120 140 160 180
400
600
800
1000
1200
1400
measurement sensor 1
measurement sensor 2
measurement sensor 3
measurement sensor 4
I norm
aliz
ed [n
A/m
m2]
Conc. NaCl [mM]
5 6 7 8 9
1100
1200
1300
1400
1500
1600
1700
I norm
aliz
ed (
nA
/mm
2)
first measurement sensor 1
first measurement sensor 2
first measurement sensor 3
first measurement sensor 4
pH
0 10 20 30 40 50 60
650
700
750
800
850
900
950
1000
1050
I no
rma
lize
d [
nA
/mm
2]
Time [min]
Glucose addition
0
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Glu
cose
con
c [m
M]