Topic 8: DNA Exercise I: Isolation Analysis Plasmid DNA

Topic 7: Cellular Genetics.Exercise II Mitosis Cell Cycle

Exp: Cell cycle onion tips

Structure of Escherichia coli.

Two strains of E.coli: pBR322 and pUC18.

Purification Plasmid DNA 1:

Purification Plasmid DNA 1:

Spin and remove most contaminants:

Purification Plasmid DNA 2:

Addition of very small volumes:

DNAse and RNAse treatment:

Preparing the Agarose gel:

Agarose Gel.

Electrophoresis:

Electrophoresis:

Electrophoretic Mobility of Nucleic Acids:

pBR322 pUC18

ET D R ET D R

pUC18 pBR322 ET D R ET R D

MITOSIS: Onion root tip.

Onion root tip.

Onion root tip.