Drug Development Solutions

Tissue Bioanalysis

Our bioanalysis team has over 10 years’ experience and a proven track record of analysing a wide variety of drugs (including small molecules to proteins and endogenous molecules) from a variety of tissues and species supporting both regulated and non-regulated studies.

We understand the complexities of tissue extraction and have a suite of techniques for effective tissue homogenisation. Our tiered approach to method establishment ensures our methods meet your project needs from exploratory studies to regulated, EMA validated status.

Modern drug discovery increasingly employs quantitative PK/PD modelling and simulation with a key focus on the quantitative measurement of drug or biomarkers in tissue compartments. Generating data to improve the knowledge and understanding of drug targets starts with the development of appropriate bioanalytical methods.

Homogenisation of tissue samples is the first step prior to sample extraction. We have an established suite of techniques to perform this. For instance, multi-tube bead and high energy ultrasonication homogenisation are often employed individually, together, or in conjunction with traditional processing

Typically we can achieve quantitation in the pg/mL to ng/mL range in our tissue LC-MS/MS assays. Matrix matching as close as possible to matrix and species is preferred but in some instances surrogate matrices made be used for tissues with limited supply.

LGC tissue types and species experience

Tissue types

• Brain• Eyes (whole

and sections) • Faeces• Fat• Heart• Intestines

• Kidney• Liver• Lung• Muscle• Nasal tissue• Nerve ganglia

• Seminal vesticles • Skin• Spinal cord• Sputum• Testes• Tumour

Species types

• Cat• Dog• Ferret• Gerbil

• Guinea pig• Hamster • Human• Monkey

• Mouse• Pig & mini pig• Rabbit• Rat

Science for a safer world

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For further information on how LGC can assist with bioanalysis, please contact us:

lgcgroup.com/enquiries

+44(0)1638 720500

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Tissue homogenisation techniquesVarious tissue types and species require different considerations and approaches to tissue homogenisation. We have developed a series of protocols utilising a whole suite of instrumentation for each tissue type and species to ensure a homogenous sample. Combining rapid and temperature controlled

homogenisation techniques, we minimise potential degradation of the analyte for the best possible analysis. As part of our capabilities, we can employ the ULTRA-TURRAX®, sonic probe disruption and the FastPrep-24TM and we are continually evaluating new instrumentation.

Our capabilitiesOperating from state-of-the-art facilities in Fordham, UK, we are recognised for leadership in science and technology, with comprehensive capabilities for PK, immunogenicity and biomarkers that span LC-MS/MS through to immunoassay, cell and molecular biology.

We have an absolute commitment to the highest quality in everything we do. We are proud of our 100% success record in regulatory GLP and GCP inspections and follow the same rigorous principles and standards for all experimental work, so whether you require non-regulated bioanalysis or full regulatory studies, we are able to support your project. To find out more about our specific MS instrumentation and regulatory accreditation visit our website or contact us.

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Target engagementtissue distribution

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Exploratory Qualified Validated

Assay Type

Xue, Gao, Ji et al., Bioanalysis (2012) 4(21)

Tiered approach to method establishmentIf your project has specific requirements, we can provide a bespoke approach to method establishment to meet your needs. Depending on the regulatory application of data generated, we are able to provide tiered methods from exploratory fit for purpose methods to full method development and validation to EMA guidelines.