Tiny Little Helper for Textile Wastewater Treatment

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Tiny Little Helper for Textile Wastewater Treatment Cultivation of Special Microbes for Enhancing Wastewater Treatment Performance HKPC TechDive – Green Living 8-12-2020

Transcript of Tiny Little Helper for Textile Wastewater Treatment

Page 1: Tiny Little Helper for Textile Wastewater Treatment

Tiny Little Helper for Textile Wastewater Treatment

Cultivation of Special Microbes forEnhancing Wastewater Treatment Performance

HKPC TechDive – Green Living

8-12-2020

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HKPC Wastewater Projects in PRC

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Current Situation in Textile Industry

GB4287-2012

“Discharge Standards of Water Pollutants for

Dyeing and Finishing of Textile industry”

• PRC has implemented new stringent discharge standard that

aniline compounds concentration down to below detection limit (i.e.

0.03 mg/L) in 2015.

• Most textile dyeing wastewater treatment facilities cannot remove

aniline compounds effectively to meet the limit.

• Even though some textile dyeing factories have eliminated all

aniline compounds discharge in the production processes, aniline

compounds were still found in the effluent discharge.

DischargeConcentration

Detection Limit (i.e. 0.03 mg/L)

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Existing Wastewater Treatment Method

in Industries

DecolorisationHydrolysis AerobicTextile Dyeing Wastewater

EffluentDischarge

Chemical coagulation

Contact Aeration 接觸好氧

PAC聚硅鋁鐵

Bleaching 漂水

Decolorizing agent脫色劑

Coagulation tank 物化 Anaerobic 厭氧

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Existing Wastewater Treatment Method

in Industries

High chemical cost in

Physiochemical Treatment

High operation cost in

frequent replacement of

carbon filter

High energy cost in aeration

in biological process with

typical biomass composition

Biomass is not selective for

target pollutants removal

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Conventional Bioreactor Design

and Cultivation Process

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Know-how of DNA identification, Cultivation, Enrichment and

Immobilisation of specialised microbes developed.

Isolation

CultivationIdentification ImmobilisationEnrichment

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Our Approach

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Our Approach

Light Microscope(Colony: ~2.5mm diameter)

Electron Microscope (Bacteria: 2.5 µm long)

Next Generation DNA Sequencing

(whole genome)

Traditional Sequencing(DNA: 2.5 nm diameter)

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Identification

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Lab-scaleCultivation & Enrichment

Taxonomic identification of isolated microbial

strains

Studies on aniline-degrading activity and decolorising activity of

the specialised microbial strains

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Isolation and Cultivation

Our Approach

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Selection Process:

By providing favourable driving force

Removal of undesirable species

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Enrichment

Our Approach

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Immobilisation

Immobilisation at controlled conditions

Biofilm of specialised microbes can be formed in around 10 days

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Our Approach

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MBBRSedimentation

Aerobic

Anoxic

Influent

TankBy-pass

Bioreactor with Specialised microbes

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Application in Existing Wastewater Treatment

Our Approach

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On-site BulkCultivation & Enrichment

Major Factors:

Specific nutrient utilisation ability and

specific metabolic properties

Driving force

Selective intensification

Immobilisation as biofilm on carriers

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Cultivation and Enrichment of Specialised Microbes

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Aniline compounds in textile dyeing wastewater can be treated solely by biological process

Acclimisation of bacteria can be enhanced by not only engineering approach but also DNA approach

Treatment process can be enhanced by improving the microbial population in the wastewater treatment units

Cultivation and Enrichment of Specialised Microbes

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Currently, no similar application of DNA identification and

offline cultivation system to provide specialised microbes;

Conventional cultivation approach is time-consuming

which leads to long start-up period of new establishment

and long system stabilisation period of existing factory in

plant upgrading;

Skilled and experienced workers are required to be

deployed to the location of new factories for testing &

commissioning.

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Cultivation and Enrichment of Specialised Microbes

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• Shorten start-up time

• Less labour intensive• Less disturbance to plant operation

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Cultivation and Enrichment of Specialised Microbes - Potential Applications

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Acknowledgement

This work was supported by ITF project grant from ITC via

Hong Kong Research Institute of Textiles and Apparel

(HKRITA) (2017 – 2019).

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Hong Kong Productivity Council香港生產力促進局

HKPC Building, 78 Tat Chee Avenue, Kowloon, Hong Kong

香港九龍達之路78號生產力大樓

+852 2788 5678 www.hkpc.org