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SPICA 2004

International Symposium on Preparative andIndustrial Chromatography and Allied Techniques

October 17-20, 2004Aachen/Germany

CONTENTPage

2Exhibitor Seminars

Exhibitor List 2

Programme at a Glance 3

Workshops 4

Lecture Programme

Generic solutions in Preparative Chromatography

Stationary and Mobile phase screening models 5

Supercritical Fluid Chromatography 6

Simulated Moving Beds: ModellingOptimisation and Operation of Industrial Multi Column ChromatographySystemsTechnologies for Preparative Chromatographic Applications 6

Allied Techniques: Part 1 - Membrane Technology 7

Allied Techniques: Part 2 - Liquid - Liquid Distribution - Hybrid Processes 8

Joint Conference Day with ISPPP 2004Stationary Phases and Column Characteristics 8Value Generation in Biopharmaceutical Industry by Efficiency, Economy,Ecology, Safety 10

Poster Programme 11

Last-Minute-Poster 19

(as of September 12, 2004)

The abstracts have been supplied by the authors and are reproduced without corrections.TIB/UB Hannover 89127 530 886

Workshops

Sunday, October 17, 2004

Page

14:00 Workshop 1Preparative HPLC: Where chemists and engineers meet 20Chair: Jochen Kinkel

Workshop 3Toolbox to understand Simulated Moving Beds 21Chair: Marco Mazzotti

15:30 Coffee Break

16:00 Workshop 2From leads to production - how chromatography is scaled-up 20Chair: Jochen Strube

Workshop 4Supercritical Fluid Chromatography 22Chair: Olivier Ludemann-Hombourger

18:00 Welcome Reception- 20:00

ProceedingsWe thank ELSEVIER for publishing the proceedings of SPICA 2004 in theJournal of Chromatography A and for their kind support of the event.

Lecture ProgrammeMonday, October 18, 2004

8:30 Opening Page

Generic solutions in Preparative Chromatography - Stationary andMobile phase screening modelsChairs: Jochen Kinkel, Fachhochschule Nurnberg/Dand Domingo Sanchez, Eka Chemicals, Bohus/S

8:40 Keynote Lecture 23Preparative chiral stationary phases for the separation ofstereoisomeres: What do we need more?E.R. Francotte, Novartis Pharma AG, Basel/CH

9:20 Solvent versatility and preparative potential of new immobilized 24polysaccharide-derived chiral stationary phases for HPLCT. Zhang, M. Schaeffer, P. Franco. CHIRAL TECHNOLOGIESEUROPE, Illkirch/F

9:45 Fast and automated determination of chromatographic parameters 25for the purification of proteins with the PPS-SystemH. Schluter, J. Thiemann, J. Jankowski, S. Kurzawski, Charite, Berlin/D

10:10 Rapid parameter estimation for a directed process development 26using high-throughput screeningP. Schulze Wierling. J. Hubbuch, Forschungszentrum Julich GmbH/D

10:35 Coffee Break with Exhibition and Posters

11:00 New generic method for phase screening in biochromatography 27M. Lohrmann. University of Dortmund/D; M. Schulte, Merck KGaA,Darmstadt/D; J. Strube, Bayer Technology Services GmbH,Leverkusen/D

11:25 Adsorbed solution models for prediction of normal-phase chroma- 28tography process with varying composition of the mobile phaseW. Piatkowski, Rzeszow University of Technology/PL; I. Petrushka,National University "Lvivska Politechnika", Lviv/UA; D. Antos. RzeszowUniversity of Technology/PL

11:50 Theoretical definition of the partition coefficient for the description 29of experimental inverse size exclusion chromatography:Applications with parallel pore and pore network modelsB.A. Grimes. C. du Fresne von Hohenesche, M. Quaglia, JohannesGutenberg-Universitat Mainz/D; D. Lubda, Merck KGaA, Darmstadt/D;K.K. Unger, Johannes Gutenberg-Universitat Mainz/D

12:15 Lunch Break and Exhibitor Seminars

13:45 Poster Afternoon

15:25 Coffee Break with Exhibition and Posters

Lecture ProgrammeMonday, October 18, 2004

Supercritical Fluid Chromatography PageChairs: Olivier Ludemann-Hombourger, NOVASEP S.A.S., Pompey/Fand Gerald Terfloth, GlaxoSmithKline, King of Prussia, PA/USA

16:00 Keynote Lecture 30Supercritical fluid chromatography: Meeting the needs of drugdiscoveryG.D. Jonas, GlaxoSmithKline Pharmaceuticals, Harlow/UK

16:40 Supercritical Fluid Chromatography (SFC): From analysis to 31purification in new drugs discoveryD. Berger, Mettler-Toledo AutoChem, St-Cergue/CH

17:05 Preparative separation of tocochromanols with supercritical fluid 32chromatographyS. Peper. M. Johannsen, G. Brunner, TU Hamburg-Harburg/D

17:30 - Supercritical Fluid Simulated Moving Bed (SF-SMB) 3417:55 enantioseparation of 1 -phenyl-1 -propanol

A. Raiendran. Swiss Federal Institute of Technology, Ziirich/CH;S. Peper, M. Johannsen, TU Hamburg-Harburg/D; M. Mazzotti,M. Morbidelli, Swiss Federal Institute of Technology, Zurich/CH

19:00 Conference DinnerLocation: The Coronation Hall, Rathaus/MarktMeeting Point: 18:50 in front of the city hall

Lecture ProgrammeTuesday, October 19, 2004

Simulated Moving Beds: Modelling - Optimisation and Operation ofIndustrial Multi Column Chromatography Systems - Technologiesfor Preparative Chromatographic ApplicationsChairs: Massimo Morbidelli, ETZ Zurichand Eric R. Francotte, Novartis Pharma AG, Basel/CH

8:40 Keynote Lecture 35Current and future trails in production scale by multicolumncontinuous chromatographyM. Hamende, UCB Pharma S.A., Braine-I' Alleud/B

9:20 Optimal operation of simulated moving bed units by on-line 36optimization based feedback controlG. Erdem, S. Abel, M. Amanullah, M. Morari, M. Mazzotti, M. Morbidelli,Swiss Federal Institute of Technology, Zurich/CH

9:45 An industrial view on the unusual adsorption behavior of some 38racemates - separating enantiomers when simulations failM. Juza, CarboGen AG, Aarau/CH

Lecture ProgrammeTuesday, October 19, 2004

Page

10:10 Application potentials of electrochemical SMB-reactors 39M. Michel. H. Schmidt-Traub, Universitat Dortmund/D; R. Ditz,M. Schulte, Merck KGaA, Darmstadt/D; J. Kinkel, W. Stark, Georg-Simon-Ohm Fachhochschule Nurnberg/D; M. Kupper, Institut fur Mikro-technik Mainz/D; M. Vorbrodt, Chemisches Laboratorium Dr. Vorbrodt,Gatersleben/D

10:35 Coffee Break with Exhibition and Posters

11:00 Efficient optimization of simulated moving bed processes 41A. Toumi. S. Engell, Universitat Dortmund/D; M. Diehl, H.G. Bock,J. Schloder, Universitat Heidelberg/D

11:25 A new multicolumn continuous chromatographic process 43integrating a concentration step in the recycling loopO. Ludemann-Hombourger, NOVASEP SAS, Pompey/F; M. Bailly,S. Abdelmoumen, Laboratoire des Sciences du Genie Chimique,Nancy/F; R.M. Nicoud, NOVASEP SAS, Pompey/F; L. Muhr, Laboratoiredes Sciences du Genie Chimique, Nancy/F

11:50 The process scale separation of enantiomers exhibiting non- 44Langmuirian adsorption: A comparison of the Simulated MovingBed (SMB) and Steady State Recycling (SSR) techniquesCM. Grill. M. Zeng, Pfizer Inc., Groton, CT/USA; M. Juza, CarboGenLaboratories AG, Aarau/CH

12:15 Lunch Break and Exhibitor Seminars

Allied Techniques: Part 1 - Membrane TechnologyChairs: Andreas Seidel-Morgenstern, Universitat Magdeburg/Dand Colin Strawson, AstraZeneca, Macclesfield/UK

13:45 New chiral polysulfones for enantiomer separation 45B. Gutierrez. C. Minguillon, University of Barcelona/E

14:10 MAAS: An adsorptive technology for the agri-food industry 47K. Merck, M. Litjens, Agrotechnology and Food Innovations B.V.,Wageningen/NL; J.-H. Hanemaaijer, TNO Milieu Energie enProcesinnovatie, Apeldoom/NL

14:35 Virus purification using adsorptive membranes 48R. Wickramasinghe, Colorado State University, Fort Collins, CO/USA,MPI fur Dynamik komplexer technischer Systeme, Magdeburg/D;B. Han, Colorado State University, Fort Collins, CO/USA; U. Reichl,MPI fur Dynamik komplexer technischer Systeme, Magdeburg/D

15:00 Continuous product removal from enzymatic aldol reactions 50S. Makart, M. Bechtold, S. Panke, Swiss Federal Institute of Technology,Zurich/CH

15:25 Coffee Break with Exhibition and Posters

Lecture ProgrammeTuesday, October 19, 2004

Allied Techniques: Part 2 - Liquid - Liquid Distribution - PageHybrid Processes

Chairs: Marco Mazzotti, ETH Zurich/CHand Reinhard Ditz, Merck KGaA, Darmstadt/D

16:00 Keynote Lecture 51A new concept of centrifugal partition chromatography for highperformance continuous purificationA. Foucault. J. Legrand, Nantes University, Saint Nazaire/F;L. Marchal, Universite de Technologie de Compiegne/F; F. Couillard,Armen Instrument, Saint Ave/F

16:40 Dynamic analysis of hybrid separation processes: Flowsheet- 53integration of continuous chromatography and enantioselectivecrystallisationM. Kaspereit. MPI fur Dynamik komplexer technischer Systeme,Magdeburg/D; A.W. Mahoney, University of Sheffield/UK; K. Gedicke,Otto-von-Guericke Universitat, Magdeburg/D; A. Seidel-Morgenstern,MPI fur Dynamik komplexer technischer Systeme, Magdeburg/D andOtto-von-Guericke Universitat, Magdeburg/D

17:05 Process integration: Design method for optimal coupling of 55chromatography and crystallization units for enantioseparationsJ. Strube. Bayer Technology Services, Leverkusen/D; G. Strohlein,TU Hamburg-Harburg/D; M. Schulte, Merck KGaA, Darmstadt/D

17:30 - Optimization of a simulated moving bed chromatography and 5617:55 crystallization hybrid process for Troger's base separation

M. Amanullah. S. Abel, M. Mazzotti, Swiss Federal Institute ofTechnology, Zurich/CH

18:00 Common Evening of SPICA and ISPPP - Poster Party- 21:00 by invitation of Merck KGaA, Darmstadt/D

Lecture ProgrammeWednesday, October 20, 2004 - Joint Conference Day with ISPPP 2004

7:15 Meeting the Youngsters - Breakfast for young scientistsStudents who registered for the conference will receive further informa-tion from the organisers four weeks before the conference.

8:30 Introduction

Stationary Phases and Column CharacteristicsChairs: Alois Jungbauer, Universitat fur Bodenkultur, Wien/Aand Jules Dingenen, Johnson & Johnson, Beerse/B

8:40 Keynote Lecture ISPPP 57Protein mass transfer kinetics in ion exchange media- measurements and interpretationsG. Carta, University of Virginia, Charlottesville, VA/USA

Lecture ProgrammeWednesday, October 20, 2004 - Joint Conference Day with ISPPP 2004

Page

9:20 Highly ordered mesoporous silicas in liquid phase separation 58processes - does the high order pay off?K.K. Unger. B.A. Grimes, Z. Bayram-Hahn, A.M. Lind, JohannesGutenberg-Universitat, Mainz/D; F. Fajula, A. Galarneau, J. lapichella,Ecole Nationale Superieure de Chimie de Montpelljer/F; R. Ditz,M. Schulte, Merck KGaA, Darmstadt/D

9:45 Molecular dynamics simulation studies of the transport and 59adsorption of a charged macromolecule onto a charged adsorbentsolid surface immersed in an electrolytic solutionX. Zhang, J.-C. Wang, A.I. Liapis, University of Missouri-Rolla,MO/USA; K.M. Lacki, Amersham Biosciences, Uppsala/S

10:10 Quantitative confocal analysis of protein transport phenomena. 61Influence of fluorescence labelling, fluid phase parameters andsignal attenuation in common industrial stationary phasesM. Schroder, T. Rosenbaum, T. Hermann, C. Teske, J. Hubbuch.Forschungszentrum Julich GmbH/D

10:35 Coffee Break with Exhibition and Posters

11:00 Computer toolbox for protein separation engineering 62B. Nilsson, Lund University/S

11:25 CFT types I and II, 40 urn ceramic fluoroapatite, comparison of 63dynamic binding of acidic proteins and the potential for pHgradient elutionL. Cummings, Bio-Rad Laboratories, Hercules, CA/USA

11:50 Prediction of affinity chromatography from uptake curves 64determined by shallow bed analysisR. Hahn, A. Tscheliessnig, A. Jungbauer, Austrian Center ofBiopharmaceutical Technology, Vienna/A and University of NaturalResources and Applied Life Sciences, Vienna/A

12:15 Lunch Break and Exhibitor Seminar ISPPP

13:45 Reactive gelation: A new process for the production of 65macro-porous materialsN. Marti, F. Quattrini, A. Butte. M. Morbidelli, Swiss Federal Institute ofTechnology, Zurich/CH

14:10 Process development for small and large molecules - differences 67- commonalities - can we learn from each other?R. Ditz, Merck KGaA, Darmstadt/D

Lecture ProgrammeWednesday, October 20, 2004 - Joint Conference Day with ISPPP 2004

Value Generation in Biopharmaceutical Industry by Efficiency, PageEconomy, Ecology, SafetyChair: Djuro Josic, Brown University, Providence, RI/USA

14:35 Application of flow field-flow fractionation as a versatile tool for 68the evaluation of protein drug stability in complex pharmaceuticalformulationsK. Mathis. G. Winter, LMU Munchen/D; W. Fraunhofer, Abbott GmbH& Co. KG, Ludwigshafen/D

15:00 High throughput analysis of protein samples by automating 69microfluidic chip technologyT. Wulff, M. Kratzmeier, A. Rufer, M. Kuschel, M. Greiner, AgilentTechnologies GmbH & Co. KG, Waldbronn/D

15:25 Coffee Break with Exhibition and Posters

16:00 Virus purification on CIM® monolithic columns 70P. Kramberger, BIA Separation, Ljubljana/SLO; M. Ravnikar, J. Boben,N. Petrovic, National Institute of Biology, Ljubljana/SLO; M. Peterka.A. Strancar, BIA Separation, Ljubljana/SLO

16:25 Solid-phase refolding of biopharmaceutical proteins from 71inclusion bodyE.K. Lee. Hanyang University, Ansan/ROK; J.D. Lee, KITECH,Incheon/ROK

16:50 Adsorption and rate phenomena in hydrophobic interaction 72chromatography of proteinsB.C.-S. To, A.M. Lenhoff. University of Delaware, Newark, DE/USA

17:15 Keynote Lecture ISPPP 73Mesoscale simulation of peptide adsorption on reversed phasechromatography media with a lattice dipole approachK. Makrodimitris, University of Virginia, Charlottesville, VA/USA andJohn Hopkins University, Baltimore, MD/USA; Y. Xiao, J.P. O'Connell,University of Virginia, Charlottesville, VA/USA; T. Woolf, John HopkinsUniversity, Baltimore, MD/USA; E.J. Fernandez. University of Virginia,Charlottesville, VA/USA

10

Poster Programme

Poster Authors PageNo.101 Automated 2D HPLC using trap columns for the fractionation, 74

isolation and screening of natural productsC. Delmotte, Gilson International B.V., Den Haag/NL; J. Stevens,A. Hamstra, T. Hegeman, N. Popp, L. Roennegurg, Gilson Inc.,Middleton, WI/USA

102 Development of PrepHPLC within Solvay Pharmaceuticals 75J. Hoogendoom, Solvay Pharmaceuticals B.V., Weesp/NL

103 Automated fraction re-analysis - net gain nothing! 76U. Huber, Agilent Technologies Deutschland GmbH & Co. KG,Waldbronn/D

104 Isolation and separation of ginkgolides and bilobalide from Ginkgo 78biloba leavesS.-M. Lai. I.-W. Chen, C.-Y. Wu, National Yunlin University of Science andTechnology/RC

105 Optimisation of L-proline derivatives and applicability as chiral 79selectors in centrifugal partition chromatographyB. Delgado, E. Perez, C. Minguillon, University of Barcelona/E

106 The influence of the polyfunctional interactions between organic 81zwitter-ion eremomycin and carboxylic cationits on formingconcentration frontI.V. Polyakova, O.A. Pisarev, Russian Academy of Sciences,St. Petersburg/RUS

107 Highly sensitive and rapid normal-phase chiral screen using high- 83performance liquid chromatography - atmospheric pressure ioniza-tion tandem mass spectrometry (HPLC/MS). Enantiomeric excessdetermination and semipreparative/preparative HPLC purificationprocessesM.L. de la Puente, A. Gonzalez, P. Lopez, L. Cano, European AnalyticalTechnologies, Lilly S.A., Alcobendas/E

108 Extraction and purification of a thermostable a-amylase from 84Bacillus coagulans found in Iranian soil samplesF. Najafi, R. Sariri. Gilan University, Rasht/IR

109 "New Paths" in preparative RP-HPLC 85M, Schiell, H. Stump-Neuhaus, Aventis Pharma Germany,Frankfurt am Main/D

110 Parallel HPLC approach for solving the purification bottleneck of 86synthesized oligonucleotidesM. Schwierskott. MWG AG, Ebersberg/D; H. Gumm, Sepiatec GmbH,Berlin/D

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Poster Programme

Poster Authors PageNo.111 Purification of fish's (Abramis ballerus) blood serum cholinesterase 87

V. Tonkopii, Institute of Limnology, St. Petersburg/RUS

112 Preparation and evaluation of PEGylated Con A for solid-phase 88extractionZ. Wen, H. Helmholz, GKSS Research Center, Geesthacht/D;B. Niemeyer, GKSS Research Center, Geesthacht/D and Helmut-Schmidt-University/University of Federal Armed Forces, Hamburg/D

113 Automated method development and purification of chiral 89compoundsG.W. Yanik, R.J. Bopp, PDR-Chiral Inc., Lake Park, FL/USA

114 Isolation and purification of icariin with molecular imprinting 90technologyM. Zhang. D. Lu, Z. Liu, Tsinghua University, Beijing/PRC

115 Effect of adsorption of organic solvents on the band profiles in 91reversed-phase non-linear chromatographyI. Poplewska, D. Antos, Rzeszow University of Technology/PL

116 Determination of the dispersive surface energy of zeolites by inverse 93gas chromatography at infinite dilutionC. Bilgic. A. A§kin, Osmangazi University, Eski§ehir/TR

117 Isotherm determination with minimum sample and solvent use 94A. Felinger, University of Veszprem/H

118 Kinetic studies for sorption of amino acids using a strong anion 95exchange resin. Effect of ionic strengthM.J. Moreira, J.A. Castro, L.M. Ferreira. Universidade de Coimbra/P

119 Computer simulation to assist the robustness analysis of an ion 96exchange separation stepN. Jakobsson. Lund University/S; J.P. Axelsson, Pfizer Health AB,Strangnas/S; B. Nilsson, Lund University/S

120 Process innovations in preparative frontal displacement 97chromatography of biomoleculesO.A. Pisarev, Russian Academy of Sciences, St. Petersburg/RUS

121 Evaluation of the potential of a combination of batch and continuous 98countercurrent operation in preparative chromatographyY. Shan. G. Ziomek, A. Seidel-Morgenstern, MPI fur Dynamik komplexertechnischer Systeme, Magdeburg/D

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Poster Programme

Poster Authors PageNo.122 Analysis of modifier-solute interactions in biochromatography using 99

equilibrium theoryG. Strohlein, L. Aumann, L. Melter, M. Mazzotti, M. Morbidelli, SwissFederal Institute of Technology, Zurich/CH

123 A description of mass transfer mechanism of antitumor antracycline 101antibiotic rubomycin within heterogeneous carboxylic sorbentsA.Y. Toshchevikova. V.P. Toshchevikov, O.A. Pisarev, Russian Academyof Sciences, St. Petersburg/RUS

124 Development of separation strategies in multistage chemical 102synthesis of fine chemicalsW. Wewers. H. Schmidt-Traub, University of Dortmund/D

125 Innovation in preparative column design to achieve stable, reliable, 103and high-load RPLC columnsF. Xia. J.Y. Cavanaugh, D.M. Diehl, G. Izzo, D. Shave, M. Savaria,T. Grady, M. Wanninger, D. Ziniti, B. Francis, R. Fisk, J. Belanger,D. Morrison, Waters Corporation, Milford, MA/USA

126 Optimizing gradient elution chromatography for the purification of 104polypeptidesZ. Zhang, M. Mazzotti, M. Morbidelli, Swiss Federal Institute ofTechnology, Zurich/CH

127 The influence of inhomogeneities in the packing on the performance 105of chromatographic columns by computer tomographyD.-U. Astrath. W. Arlt, TU Berlin/D

128 Enantioseparation of amino acids on a macrocyclic glycopeptide 106column employing eluents compatible with enzyme catalysisM. Bechtold, S. Makart, S. Panke, Swiss Federal Institute of Technology,Zurich/CH

129 Affinity, metal chelate and ion exchange chromatography on 108Sartobind membrane adsorbersW. Demmer, Sartorius AG, Gottingen/D

130 Amino acids separation using micellar chromatography and 109selective complex formationL. Garcia Diez, H.-J. Bart, University of Kaiserslautern/D

131 Exchange adsorption model for multicomponent systems 112N.A. Eltekova. Y.A. Eltekov, Russian Academy of Sciences, Moscow/RUS

132 Carbohydrates LC separation on silica beds 113A.Y. Eltekov, Russian Academy of Sciences, Moscow/RUS

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Poster Programme

Poster Authors PageNo.133 Synthesis of the new type of the chromatographic polymer carriers 114

"fitted" to the target objectI.S. Garkushina, N.M. Ezhova, O.A. Pisarev, Russian Academy ofSciences, St. Petersburg/RUS

134 Molecular recognition in chromatography: A novel design for 115poly-valent stationary phasesK. Gottschall, S. Evsyukov, L. Lotzbeyer, D. Muller, M. Welter, L. Ziser,instrAction GmbH, Ludwigshafen/D

135 Potential of Microsorb for preparative applications 116R. Guilliet, Varian Consumable Products, Middelburg/NL

136 Optimization of hydrophobic interaction chromatographic resin for 117antibody purificationY. Hashimoto. E. Sakima, Y. Mitoma, Y. Yasuda, Separation Center,Tosoh Corp., Yamaguchi/J

137 New insights into hydrophobic interaction chromatography 118employing Confocal Laser Scanning Microscopy (CLSM)T. Herrmann. S. Pezold, M.-R. Kula, J. Hubbuch, ForschungszentrumJulich GmbH/D

138 Viral clearance by BioSAFE (Bioseparations using surfactant aided 119size exclusion chromatography for viral clearance)D.A. Horneman. M. Ottens, Delft University of Technology/NL;J.T.F. Keurentjes, Eindhoven University of Technology/NL;L.A.M. van der Wielen, Delft University of Technology/NL

139 Comparison of the purification profile of a peptide produced by 121biosynthesis and solid phase synthesisT. Budde Hansen, C. Kornbeck, Novo Nordisk A/S, Gentofte/DK

140 New bonding technology for superior HPLC packing materials: 122Excellent peak symmetry for basic compoundsT. Urano, O. Nakajima, Daiso Co., Ltd., Hyogo/J; G. Krautz,Daiso Co., Ltd., Diisseldorf/D

142 Downstream processing of equine influenza virus 123P.P. Navak. S. Lehmann, U. Reichl, MPI fur Dynamik komplexertechnischer Systeme, Magdeburg/D

143 Minimizing inner dispersion by homogeneous packings 124W. Pfeiffer, Hamburg/D

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Poster Programme

PosterNo.

144

145

146

147

148

149

150

151

152

153

Authors Page

Analysis of different adsorbent materials and their application in 125affinity separation processesH. Rosenfeld, GKSS Research Centre, Geesthacht/D; B. Niemeyer,GKSS Research Centre, Geesthacht/D and Helmut-Schmidt-University/University of Federal Armed Forces, Hamburg/D

Flash Chromatography - from the self packed glass column to the 126pre packed flash cartridgeG. Schonenberger, Buchi Labortechnik GmbH, Essen/D

Sorbent properties and their impact on economy of preparative 128separationsM. Schulte. M. Johnck, R. Ditz, Merck KGaA, Darmstadt/D; K.K. Unger,University of Mainz/D

Functionalized cellulose membranes for affinity separation of 129biomoleculesM. Sorci. C. Bol, F. Cattoli, G.C. Sarti, University of Bologna/I

Rapid removal of DNA and host cell proteins in the purification of 130monoclonal antibodiesA. Stein, A. Kiesewetter, Merck KGaA, Darmstadt/D

Modelling chromatographic protein separation by general rate model 131and confocal laser scanning microscopyA. Susanto, K. Wekenborg, University of Dortmund/D; J. Hubbuch,Forschungszentrum Julich GmbH/D; H. Schmidt-Traub, University ofDortmund/D

Confocal laser scanning microscopy studies of protein elution in 133ion-exchange chromatographyC.A. Teske. M.-R. Kula, J. Hubbuch, Forschungszentrum Julich GmbH/D

New poly-valent molecular recognition phases for preparative 134purification in pharmaceutical development and productionK. Gottschall, S. Evsyukov, L. Lotzbeyer, D. Muller, M. Welter. L. Ziser,instrAction GmbH, Ludwigshafen/D

The characteristics of novel semi-preparative ion-exchange columns 135K. Muranaka. Y. Mitoma, Tosoh Corporation, Yamaguchi/J; Y. Hashimoto,T. Kamitika, H. Moriyama, Y. Yasuda, Tosoh Corporation, Kanagawa/J

Stochastic optimization of simulated moving bed process. 136Sensitivity analysis for isocratic and gradient operationG. Ziomek, MPI fur Dynamik komplexer technischer Systeme,Magdeburg/D; P. Antos, Rzeszow University of Technology/PL

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Poster Programme

Poster Authors PageNo.154 The Hashimoto process - a partially deintegrated chromatographic 137

reactorT. Borren. H. Schmidt-Traub, University of Dortmund/D

155 Separation of lactose from human milk oligosaccharides with 139simulated moving bed chromatographyA. Geisser. T. Hendrich, G. Boehm, B. Stahl, Numico Research Germany,Milupa GmbH, Friedrichsdorf/D

156 Parameter identification from batch experiments for use in SMB 142modelsV. Grosfils, Universite Libre de Bruxelles/B; C. Levrie, FacultePolytechnigue de Mons/B; M. Kinnaert, Universite Libre de Bruxelles/B;A. Vande Wouwer, Faculte Polytechnique de Mons/B

157 On simplified modelling approaches to SMB processes 144V. Grosfils. Universite Libre de Bruxelles/B; C. Levrie, FacultePolytechnique de Mons/B; M. Kinnaert, Universite Libre de Bruxelles/B;A. Vande Wouwer, Faculte Polytechnique de Mons/B

158 Identification based predictive control of a simulated moving bed 146process with bi-Langmuir isothermI. Song. S. Lee, H. Rhee, Seoul National University/ROK; M. Mazzotti,Swiss Federal Institute of Technology, Zurich/CH

159 Design of a simulated-moving-bed reactor using batch experiments 148and equilibrium theoryG. Strohlein. Swiss Federal Institute of Technology, Zurich/CH;R. Proplesch, DSM Nutritional Products, Chemical Process Technology,Sisseln/CH; D. Prokic, University of Belgrade/YU; M. Mazzotti,M. Morbidelli, Swiss Federal Institute of Technology, Zurich/CH

160 A novel design of SMB (Simulated Moving Bed) chromatography for 150separation of ketoprofen racemateT.H. Yoon. I.H. Kim, Chungnam National University/ROK;H. Schmidt-Traub, University of Dortmund/D

161 Supercritical Fluid Chromatography (SFC): Analysis and purification 151of chiral compounds in new drugs discoveryD. Berger, Mettler-Toledo AutoChem, Inc., St-Cergue/CH

162 Preparative unified chromatography: The use of HPLC and SFC to 152purify gram to kilogram batches of pharmaceutical ingredients withone multi-purpose instrumentT. Palcic, H. Sidhu, Thar Technologies, Inc., Pittsburgh, PA/USA

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Poster Programme

PosterNo.

163

164

165

166

167

168

169

170

171

Authors Page

Enantionseparation under non-linear conditions in supercritical fluid 153chromatographyA. Rajendran, J. Kluge, S. Ottiger, M. Mazzotti, M. Morbidelli, SwissFederal Institute of Technology, Zurich/CH

Recycling of CO2 on a supercritical fluid chromatography (SCF) pilot 154unitW. Maiewski. O. Ludemann-Hombourger, NOVASEP SAS, Pompey/F

Polar mobile phase design and bonding chemistries for the efficient 155analytical or preparative separation of drug enantiomersP.M. Wallworth, T. Beesley, Advanced Separation Technologies,Congleton/UK and Whippany, NJ/USA

Experimental and theoretical analysis of potential and limits of 156applying different solvents for the mobile phase and for sampleinjectionK. Gedicke, Otto-von-Guericke-Universitat Magdeburg/P; M. Tomusiak,P. Antos, Rzeszow University of Technology/PL; A. Seidel-Morgenstem.Otto-von-Guericke-Universitat Magdeburg/P und MPI fur Dynamikkomplexer technischer Systeme, Magdeburg/D

Partitron 25 a multi-purpose industrial centrifugal partition 157chromatograph: Rotor design and preliminary results on efficiencyand stationary phase retentionR. Maroraff. O. Intes, PARTUS Technologies SA, Reims/F; J.-H. Renault,University of Reims Champagne Ardenne/F; P. Garret, Couturier SAS,Clairegoutte/F

Thermal effect on flavor retention in microencapsulated acerola pulp 158H.S. Ribeiro. University Karlsruhe (TH)/D; C.A. Gasparetto, F. Carraro,A. Sartoratto, State University of Campinas/BR

Modeling of temperature profiles in capillary electrochromatography 160as a feasibility study for semipreparative applicationsT. Schneider. A. Boehme, TU Berlin/D; W. Arlt, Friedrich-Alexander-University Erlangen-Nuremberg/D

Selection of column chemistry to improve the loading of 161harmaceutical compounds on RPLC columnsF. Xia, J.Y. Cavanaugh, U.D. Neue, D.M. Diehl, Waters Corporation,Milford, MA/USA

Separation of proteins from potato fruit juice using ion exchange 162membrane chromatographyV. Alt, F. Menzel, C. Kasper, University of Hannover/D; M. Lotz,Emsland-Starke GmbH, Emlichheim/D; R. Ulber, T. Scheper, Universityof Hannover/D

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Poster Programme

Poster Authors PageNo.172 Applying mass balances to evaluate the potential of hybrid 163

eparation processesK. Gedicke, Otto-von-Guericke-Universitat, Magdeburg/D; M. Kaspereit,MPI fur Dynamik komplexer technischer Systeme, Magdeburg/D;A. Seidel-Morgenstern, Otto-von-Guericke-Universitat, Magdeburg/D undMPI fur Dynamik komplexer technischer Systeme, Magdeburg/D

173 Negatively cooperative binding of melittin to neutral phospholipid 164vesiclesF. Torrens, Universitat de Valencia/E

174 Macro-prep ceramic hydroxyapatite 40 urn optimized packing 165method for GelTec columnsL Cummings, Bio-Rad Laboratories, Hercules, CA/USA; P. Vidalinc,Bio-Rad Laboratories, Riom/F

175 Modeling of the HPLC chiral-separation of benzodiazepines on a new 166poly-brush type chiral stationary phaseA. Cavazzini. F. Dondi, University of Ferrara/I; A. Felinger, University ofVeszprem/H; C. Villani, R. Rompietti, F. Gasparrini, University of Rome"La Sapienza'VI

176 Analysis of ester hydrolysis reactions in a chromatographic reactor 167using equilibrium theoryA. Kienle, Otto-von-Guericke-Universitat, Magdeburg/D und MPI furDynamik komplexer technischer Systeme, Magdeburg/D; S. Gruner,Universitat Stuttgart/D; P.T. Mai, T.D. Vu, Otto-von-Guericke-Universitat,Magdeburg/D; A. Seidel-Morgenstern, Otto-von-Guericke-Universitat,Magdeburg/D und MPI fur Dynamik komplexer technischer Systeme,Magdeburg/D

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Last-Minute-Poster

Poster Authors PageNo.LMP 177 Design of a nonlinear distributed parameter observer for an SMB 169

processC. Levrie, A. Vande Wouwer, Faculte Polytechnique de Mons/B;V. Grosfils, M. Kinnaert, Universite Libre de Bruxelles, Brussels/B

LMP 178 Continuous chromatographic separation of n-Boc-Baclofen-Lactam 170in simulated moving bed using polysaccharide carbamate as chiralstationary phaseV. de Veredas, M.J.S. Carpes, C.R.D. Correia, C.C. Santana,Universidade Estadual de Campinas/BR

LMP 179 Fully automated high throughput quantitation of compound 171libraries using LC-MS-CLNDW. Verhaegen. A. Woodhouse, Agilent TechnologiesSales & Services GmbH & Co. KG, Waldbronn/D

LMP 180 Chromatographic study of adsorption of n-alkanes on moleculer 172sieve MgYC. Bilgic, Osmangazi University, Eski§ehir/TR

LMP 181 Agilent Prep silica and C18 - optimized phases for preparative 173chromatographyF. te Welscher. U. Jegle. M. Joseph, Q. Wang, Agilent TechnologiesSales & Services GmbH & Co. KG, Waldbronn/D

LMP 182 The use of large scale chromatography to accelerate 174pharmaceutical R&DL. Miller. Amgen, Cambridge, MA/USA; CM. Grill, Pfizer Inc,Groton, CT/USA

LMP 183 Single-column simulated-moving-bed process with recycle lag 175J.P.B. Mota, J.M.M. Arauio. Universidade Nova de Lisboa, Caparica/P

LMP 184 Mass-directed purification using multi-mode ionisation 176R. Clearv. P. Lefebvre, R. Plumb, W.B. Potts III, Waters Corporation,Milford, MA/USA

LMP 185 Isolation and purification by preparative chromatography of 177reaction products of two antineoplastic drugs with ozone inwater mediumS. Padron. A. Ramos, A. Visozo, V Montero, B. Lopez, M. Torres,Center for Research and Development of Drugs, Havana/C

LMP 186 Semipreparative and preparative chromatography in the stability 178studies of drugs. A case study: Isolation and purification ofdegradation products of two antiviral drugsS. Padron. A. Londaitsbehere, B. Lopez, M. Torres, Center for Researchand Development of Drugs, Havana/C

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