Three Fates of Pyruvate Pyruvate acetyl-CoA Occurs in mitochondria Produce CO 2 and NADH + H +...

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Three Fates of Pyruvate Pyruvate acetyl-CoA Occurs in mitochondria Produce CO 2 and NADH + H + Pyruvate Dehydrogenase Aerobic **Acetyl-CoA used in the TCA cycle

Transcript of Three Fates of Pyruvate Pyruvate acetyl-CoA Occurs in mitochondria Produce CO 2 and NADH + H +...

Page 1: Three Fates of Pyruvate Pyruvate acetyl-CoA Occurs in mitochondria Produce CO 2 and NADH + H + Pyruvate Dehydrogenase Aerobic **Acetyl-CoA used in the.

Three Fates of Pyruvate Pyruvate acetyl-CoA

Occurs in mitochondria Produce CO2 and NADH + H+

Pyruvate Dehydrogenase Aerobic **Acetyl-CoA used in the TCA cycle

Page 2: Three Fates of Pyruvate Pyruvate acetyl-CoA Occurs in mitochondria Produce CO 2 and NADH + H + Pyruvate Dehydrogenase Aerobic **Acetyl-CoA used in the.

Three Fates of Pyruvate Pyruvate Lactate

Produce CO2 and NAD+

Homolactic fermentation Anaerobic

Pyruvate Ethanol Produce CO2 and NAD+

Alcoholic fermentation Anaerobic

Page 3: Three Fates of Pyruvate Pyruvate acetyl-CoA Occurs in mitochondria Produce CO 2 and NADH + H + Pyruvate Dehydrogenase Aerobic **Acetyl-CoA used in the.

Citric Acid Cycle 8 reactions Oxidize acetyl groups

Acetyl groups come from many sources (not just carbohydrates)

“Hub” of cellular metabolism Intermediates are precursors for the biosynthesis

of other compounds 3 NAD++FAD+GDP+Pi+acetyl-CoA

3 NADH+FADH2+GTP+CoA+2 CO2

Page 4: Three Fates of Pyruvate Pyruvate acetyl-CoA Occurs in mitochondria Produce CO 2 and NADH + H + Pyruvate Dehydrogenase Aerobic **Acetyl-CoA used in the.

Citric Acid Cycle Reaction 1

Oxaloacetate (OAA) + acetyl-CoA + H2O Citrate + CoASH

Citrate synthase Condensation

Reaction 2 Citrate Isocitrate Aconitase Isomerization

Page 5: Three Fates of Pyruvate Pyruvate acetyl-CoA Occurs in mitochondria Produce CO 2 and NADH + H + Pyruvate Dehydrogenase Aerobic **Acetyl-CoA used in the.

Citric Acid Cycle Reaction 3

Isocitrate + NAD+ Ketoglutarate (KG) + NADH + H+ + CO2

Isocitrate dehydrogenase Dehydrogenation and oxidative decarboxylation

Reaction 4 Ketoglutarate + CoASH + NAD+

Succinyl-CoA + NADH + H+ + CO2

Ketoglutarate dehydrogenase Dehydrogenation and oxidative decarboxylation

Page 6: Three Fates of Pyruvate Pyruvate acetyl-CoA Occurs in mitochondria Produce CO 2 and NADH + H + Pyruvate Dehydrogenase Aerobic **Acetyl-CoA used in the.

Citric Acid Cycle Reaction 5

Succinyl-CoA + GDP + Pi Succinate +GTP + CoASH

Succinyl-CoA synthetase Substrate level phosphorylation via coupling

reactions Reaction 6

Succinate + FAD Fumarate + FADH2

succinate dehydrogenase Dehydrogenation

Page 7: Three Fates of Pyruvate Pyruvate acetyl-CoA Occurs in mitochondria Produce CO 2 and NADH + H + Pyruvate Dehydrogenase Aerobic **Acetyl-CoA used in the.

Citric Acid Cycle Reaction 7

Fumarate + H2O Malate Fumarase Hydration

Reaction 8 Malate Oxaloacetate Malate dehydrogenase Dehydrogenation

Page 8: Three Fates of Pyruvate Pyruvate acetyl-CoA Occurs in mitochondria Produce CO 2 and NADH + H + Pyruvate Dehydrogenase Aerobic **Acetyl-CoA used in the.

ATP Production NADH : ATP 1 : 3

FADH2 : ATP 1 : 2

GTP : ATP 1 : 1

Page 9: Three Fates of Pyruvate Pyruvate acetyl-CoA Occurs in mitochondria Produce CO 2 and NADH + H + Pyruvate Dehydrogenase Aerobic **Acetyl-CoA used in the.

Summary of ATP ProducedProducts Net ATP

Glycolysis

-2 ATP -2 ATP

+4 ATP +4 ATP

+2 NADH +6 ATP

PVA DH +2 NADH +6 ATP

TCA cycle

+6 NADH +18 ATP

+2 FADH2 +4 ATP

+2 GTP +2 ATP

Total 38 ATP


ENERGY METABOLISM · protein synthesis, b. they are with the resulting carbon skeletons being degraded by the liver pyruvate, acetyl CoA, or TCA cycle intermediates, these metabolites

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Inhibition of pyruvate dehydrogenase kinase 2 protects ... · TCA cycle to generate ATP to meet hepatic energy demands (4). Whether acetyl-CoA produced by β-oxidation forms ketone

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Table of contents - ULTRA KULTUREGlycerol acids acids Glucose ADP Pyruvate O,toso/ Mitochondrion ADP ADP Fatty acids Acetyl COA Amino acids CITRIC ACID CYCLE ELECTRON TRANSPORT SYSTEM

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Kinetic Flux Profiling Elucidates Two Independent Acetyl-CoA ...

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ANSC 619 PHYSIOLOGICAL CHEMISTRY OF LIVESTOCK SPECIES ...€¦ · A. Conversion of pyruvate to acetyl CoA 1. Pyruvate is decarboxylated by pyruvate dehydrogenase at the inner mitochondrial

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Gut MucosalenzymeactivityMucosalenzymeactivityforbutyrate oxidation Butyric acid CH3.CH2.CH2.COOH ATP AMP Butyryl CoA Crotonyl CoA Hydroxybutyryl Cl NA NAI Acetoacetyl CoA Acetyl CoA

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3.6 Krebs Cycle 3.6a Pyruvate Oxidation 3.6b Krebs Cycle ...dalton.med.miami.edu/lab/teaching/BMB401/pdfs/3.6-KrebsCycle.pdf · Synopsis 3.6a - A major source of acetyl group of acetyl-CoA

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bjc alcohol 27-1-2017 VITALpcbjcampbl/Alcohol_handout1.pdf · ALCOHOL METABOLISM Lactate dehydrogenase Lactate Pyruvate Acetyl CoA CO2 + H2O i.e. Citric acid cycle GTP GMP The first

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Cellular Respiration Part IV: Oxidative Phosphorylation · for use in biological processes. g. ... oxidation Acetyl CoA Glycolysis Glucose Pyruvate Oxidative phosphorylation: electron

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CHAPTER 5...Citric acid cycle Pyruvate oxidation Acetyl CoA Glycolysis Glucose Pyruvate Oxidative phosphorylation: ...

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Acetyl‐CoA Carboxylase Inhibition Reverses NAFLD and ...natap.org/2018/AASLD/Goedeke_et_al-2018-Hepatology.pdf · 2197 Hepatology, Vol. 68, No. 6, 2018 Acetyl-CoA Carboxylase Inhibition

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Chapter 16.1: Production of Acetyl-CoA

Chapter 16.1: Production of Acetyl-CoA

Pyruvate Kinase M2: Multiple Faces for Conferring Bene on ... · Pyruvate can be converted to lactate or to acetyl-CoA, and such direction is determined by the enzymatic activ-ity

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Lipid Biosynthesis - Western Oregon Universityguralnl/451Lipid BiosynthesisI.pdfTransfer of Acetyl CoA • Acetyl CoA from catabolism of carbohydrates and amino acids is exported from

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Course: Nutrition and Metabolism of carbon skeleton.pdf · Pyruvate Acetyl-CoA a-Ketoglutarate Succinyl-CoA Fumarate Oxaloacetate Acetoacetate Glucose Ile, Met, Val Gln, Glu, His,

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Regulation of Carbohydrate Metabolism · Regulation of Carbohydrate Metabolism Acetyl-CoA/Pyruvate Pyruvate/PEP F6P/FBP: Fru 2,6P 2 Glc/Glc6P Glycogen Anaplerotic reactions Regulation

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Review Article Roles of Pyruvate, NADH, and Mitochondrial ...downloads.hindawi.com/journals/jdr/2015/512618.pdfPyruvate TCA cycle Glycolysis Acetyl-CoA NADH ANT ADP ATP Insulin Insulin

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The role of acetyl-CoA carboxylase activity in normal ...

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Cloning and of acetyl-CoA synthesis Clostridium CO and

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