Thioglycollate Broth
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Aerobic and anaerobic bacteria can be identified by
growing them in test tubes of thioglycollate broth:
1: Obligate aerobes need oxygen because they
cannot ferment or respire anaerobically. They
gather at the top of the tube where the oxygen
concentration is highest.
2: Obligate anaerobes are poisoned by oxygen, so
they gather at the bottom of the tube where the
oxygen concentration is lowest.
3: Facultative anaerobes can grow with or without
oxygen because they can metabolise energy
aerobically or anaerobically. They gather mostly at
the top because aerobic respiration generates more
ATP than either fermentation or anaerobic
respiration.
4: Microaerophiles need oxygen because they
cannot ferment or respire anaerobically. However,
they are poisoned by high concentrations of oxygen.They gather in the upper part of the test tube but not
the very top.
5: Aerotolerant organisms do not require oxygen as
they metabolise energy anaerobically. Unlike
obligate anaerobes however, they are not poisoned
by oxygen. They can be found evenly spread
throughout the test tube.
Thioglycollate broth
medium is recommended
to isolate strict anaerobes
should an anaerobic
infection be suspected.[2]
Thioglycollate brothFrom Wikipedia, the free encyclopedia
Thioglycollate broth is a multi-purpose, enriched differential
medium used primarily to determine the oxygen requirements of
microorganisms. Sodium thioglycolate in the medium consumes
oxygen and permits the growth of obligate anaerobes.
[1]
This,combined with the diffusion of oxygen from the top of the broth
produces a range of oxygen concentrations in the media along its
depth. The oxygen concentration at a given level is indicated by a
redox sensitive dye like resazurine that turns pink in the presence of
oxygen.
This allows the differentiation of
obligate aerobes, obligate anaerobes,
facultative anaerobes,
microaerophiles, and aerotolerant
organisms. For example, obligately
anaerobic Clostridium species will be
seen growing only in the bottom ofthe test tube.
Thioglycollate broth is also used to
recruit macrophages to the peritoneal
cavity of mice when injected
intraperitoneally.[3] It recruits
numerous macrophages, but does not
activate them.[3] The thioglycollate-
induced peritonitis in mice is used as a
model to study for potential anti-
inflammatory action of investigated
test compounds.[4]
References
1. ^ Cain, Donna. "Fluid Thioglycollate
Broth" (http://iws2.collin.edu/dcain/CCCCD%
20Micro/thioglycollatebroth.htm). Collin College.
2. ^ Cheesbrough, Monica (2006). District Laboratory Practice in
Tropical Countries, Part 2 (http://books.google.es/books?id=Yjv-n5u6S7gC). Cambridge University Press. p. 126.
ISBN 0-521-67631-2. Retrieved 2009-09-14.
3. ^ a b Leijh PC; van Zwet TL; ter Kuile MN; van Furth R (November 1984). "Effect of thioglycolate on phagocytic and
microbicidal activities of peritoneal macrophages" (https://www.ncbi.nlm.nih.gov/pmc/articles/PMC261553). Infection and
Immunity 46 (2): 448–452. PMC 261553 (https://www.ncbi.nlm.nih.gov/pmc/articles/PMC261553). PMID 6500699
(https://www.ncbi.nlm.nih.gov/pubmed/6500699).
4. ^ Fakhrudin N, Waltenberger B, Cabaravdic M, Atanasov AG, Malainer C, Schachner D, Heiss EH, Liu R, Noha SM,
Grzywacz AM, Mihaly-Bison J, Awad EM, Schuster D, Breuss JM, Rollinger JM, Bochkov V, Stuppner H, Dirsch VM.
Identification of plumericin as a potent new scaffold inhibitor of the NF-kB pathway with anti-inflammatory activity in vitro
and in vivo.
(http://onlinelibrary.wiley.com/doi/10.1111/bph.12558/abstract;jsessionid=8BFCA3DB1F259C4002C794232721151B.f01t04)
Br J Pharmacol. 2013 Dec 16. doi: 10.1111/bph.12558. PubMed PMID: 24329519.
(http://www.ncbi.nlm.nih.gov/pubmed/24329519)
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