The+ Zebrafish+ Project
-
Upload
guest3b77a0 -
Category
Business
-
view
735 -
download
0
Transcript of The+ Zebrafish+ Project
The Zebrafish ProjectThe Zebrafish Project
Katie HengenKatie Hengen
Lesley SullivanLesley Sullivan
ZebrafishZebrafish
Danio rerio, Danio rerio, freshwater freshwater fishfish
Originally from Ganges Originally from Ganges River in East India and River in East India and BurmaBurma
Found in slow streams Found in slow streams and rice paddiesand rice paddies
Excellent model for Excellent model for studying vertebrate studying vertebrate development and development and geneticsgenetics
Used in ResearchUsed in Research
Dr. George Streisinger Dr. George Streisinger (1970’s) determined (1970’s) determined usefulnessusefulnessSimilar embryonic Similar embryonic development to humansdevelopment to humansHuman genome known, Human genome known, easy to work with similar easy to work with similar genesgenesSome mutants known to Some mutants known to develop and duplicate develop and duplicate certain diseases common certain diseases common to humansto humans
Ideal ModelsIdeal Models
Vertebrates Vertebrates – Similar biological traits to Similar biological traits to
humanshumansGenesGenes
Developmental processesDevelopmental processes
AnatomyAnatomy
PhysiologyPhysiology
BehaviorsBehaviors
– Better genetic models than Better genetic models than commonly used commonly used invertebrates (fruit flies, invertebrates (fruit flies, worms)worms)
Ideal ModelsIdeal Models
Certain features make Certain features make easy to maintain, easy to maintain, manipulate, and observemanipulate, and observe– Do well in many Do well in many
environmentsenvironments– Small size Small size – Ability to be kept in large Ability to be kept in large
numbersnumbers– Easy breedingEasy breeding
Eggs externally fertilizedEggs externally fertilizedProduced regularly in Produced regularly in large numberslarge numbersNon-adhesiveNon-adhesive
– Easy to trace development Easy to trace development of individual cellsof individual cells
Ideal ModelsIdeal ModelsLarge quantities of eggsLarge quantities of eggsDevelop outside mother’s Develop outside mother’s bodybody– Easily accessible, Easily accessible,
contrasting micecontrasting mice
Transparent embryosTransparent embryos– Observe very first cell Observe very first cell
divisiondivision– Watch development of Watch development of
internal organsinternal organs– Monitor behavior of single Monitor behavior of single
cellscells
Embryos develop quicklyEmbryos develop quickly– From single cell to fish-like From single cell to fish-like
in 24 hoursin 24 hours
Zebrafish eggs 2 hours after fertilization.
Ideal ModelsIdeal Models
Can physically Can physically manipulate embryosmanipulate embryos– Transplant cells/groups of Transplant cells/groups of
cells into host embryocells into host embryo– Fertilization manipulated so Fertilization manipulated so
embryo contains only embryo contains only mother’s genesmother’s genes
Large community of Large community of researchers work with researchers work with zebrafishzebrafish
Zebrafish in High Schools Zebrafish in High Schools
InexpensiveInexpensiveTolerant of reasonable Tolerant of reasonable amount of stressamount of stressProduce large amounts of Produce large amounts of eggs for observationeggs for observationClear embryo visual with Clear embryo visual with standard high school standard high school microscopesmicroscopesFish embryo development Fish embryo development similar to other speciessimilar to other speciesZebrafish are Zebrafish are entertaining; students entertaining; students enjoy working with themenjoy working with them
Research Using ZebrafishResearch Using Zebrafish
EmbryologyEmbryology
Collecting and analyzing mutations, Collecting and analyzing mutations, comparing to other vertebratescomparing to other vertebrates
Genetic techniques and staining reagents Genetic techniques and staining reagents developed developed
Zebrafish genomeZebrafish genome
Transgenic fishTransgenic fish
Reaching OutReaching Out
Plans unfoldingPlans unfolding
Biology students at St. Joe are VERY Biology students at St. Joe are VERY LUCKY!LUCKY!
Clay High SchoolClay High School
QuarkNet QuarkNet
NDercNDerc