The Working Cell Chapter 5 - · PDF fileChapter 5 The Working Cell-Hand up outlines-Grab a...
Transcript of The Working Cell Chapter 5 - · PDF fileChapter 5 The Working Cell-Hand up outlines-Grab a...
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Copyright © 2009 Pearson Education, Inc.
PowerPoint Lectures forBiology: Concepts & Connections, Sixth EditionCampbell, Reece, Taylor, Simon, and Dickey
Chapter 5 The Working Cell
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- Hand up outlines
- Grab a worksheet from my front
desk
- turn on a chromebook with
your lab partner, but stay at the
desks for now!
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▪ Energy is the capacity to do work and cause change
– Work is accomplished when an object is moved against an opposing force, such as friction
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Introduction: Turning on the Lights to Be Invisible
▪ Some organisms use energy-converting reactions to produce light
– Examples are organisms that live in the ocean and use light to hide themselves from predators
▪ Energy conversion involves not only energy but also membranes and enzymes
▪ So, production of light involves all of the topics covered in this chapter
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MEMBRANE STRUCTURE AND FUNCTION
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5.1 Membranes are a fluid mosaic of phospholipids and proteins
Membranes are composed of → phospholipids and proteins
– Membranes are commonly described as a fluid mosaic
– This means that the surface appears mosaic because of the proteins embedded in the phospholipids and fluid because the proteins can drift about in the phospholipids
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Phospholipidbilayer
Hydrophobic regionsof protein
Hydrophilicregions of protein
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Water
Water
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▪ Many phospholipids are made from unsaturated fatty acids that have kinks in their tails
– This prevents them from packing tightly together, which keeps them liquid
– This is aided by cholesterol wedged into the bilayer to help keep it liquid at lower temperatures
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Hydrophilichead
WATER
Hydrophobictail
WATER
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▪ Membranes contain integrins, which give the membrane a stronger framework
Integrins are proteins that attach to the extracellular matrix on the outside of the cell as well as span the membrane to attach to the cytoskeleton.
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Cholesterol
Glycoprotein
Glycolipid
Carbohydrate ofglycoprotein
Phospholipid
Microfilamentsof cytoskeleton
Integrin
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Some glycoproteins in the membrane serve as identification tags that are specifically recognized by membrane proteins of other cells
Carbohydrates that are part of the extracellular matrix are also involved in cell-cell recognition
Example: cell-cell recognition enables cells of the immune
system to recognize and reject foreign cells, such as infectious
bacteria!
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Many membrane proteins function as:
– enzymes– signal transduction– transport
Since membranes allow some substances to cross or be transported more easily than others, they exhibit
selective permeability– Nonpolar molecules (carbon dioxide and oxygen) cross easily
– Polar molecules (glucose and other sugars) do not cross easily
.
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Enzymes
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Messenger molecule
Activatedmolecule
Receptor
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5.2 EVOLUTION CONNECTION: Membranes form spontaneously, a critical step in the origin of life
Phospholipids, the key component of biological membranes, spontaneously assemble into simple membranes
– Formation of a membrane that encloses collections of molecules necessary for life was a critical step in evolution
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Water-filledbubble made ofphospholipids
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5.3 Passive transport is diffusion across a membrane with no energy investment
Diffusion is a process in which particles spread out evenly in an available space
– Particles move from an area of more concentrated particles to an area where they are less concentrated
– This means that particles diffuse down their concentration gradient
– Eventually, the particles reach equilibrium where the concentration of particles is the same throughout
*Diffusion Demo*
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▪ Diffusion across a cell membrane does not require energy, so it is called passive transport
– The concentration gradient itself represents potential energy for diffusion
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Molecules of dye Membrane Equilibrium
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Two differentsubstances Membrane Equilibrium
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5.4 Osmosis is the diffusion of water across a membrane
– Water moves across membranes in response to solute concentration inside and outside of the cell by a process called osmosis
– Osmosis will move water across a membrane down its concentration gradient until the concentration of solute is equal on both sides of the membrane
It is crucial for cells that water moves across their membrane…
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DO NOW:
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Selectivelypermeablemembrane
Solutemolecule
Lowerconcentration
of solute
H2O
Solute molecule withcluster of water molecules
Net flow of water
Watermolecule
Equalconcentration
of solute
Higherconcentration
of solute
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5.5 Water balance between cells and their surroundings is crucial to organisms
▪ Tonicity is a term that describes the ability of a solution to cause a cell to gain or lose water
Isotonic: indicates that the concentration of a solute is the same on both sides so it is balanced both inside and out
Hypertonic: indicates that the concentration of solute is higher outside the cell so it SHRINKS
Hypotonic: indicates a higher concentration of solute inside the cell so it EXPANDS / SWELLS
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Think hippo for “hypotonic”
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▪ Many organisms are able to maintain water balance within their cells by a process called osmoregulation
– This process prevents excessive uptake or excessive loss of water
– Plant, prokaryotic, and fungal cells have different issues with osmoregulation because of their cell walls
Video: Paramecium Vacuole
Video: Chlamydomonas
Video: Turgid Elodea
Video: Plasmolysis
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Isotonic solution
(B) Lysed (C) Shriveled
(D) Flaccid (E) Turgid (F) Shriveled
Hypertonic solutionHypotonic solution
Plantcell
Animalcell
(A) Normal
Plasmamembrane
(plasmolyzed)
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5.6 Transport proteins may facilitate diffusion across membranes
▪ Many substances that are necessary for viability of the cell do not freely diffuse across the membrane
– They require the help of specific transport proteins called aquaporins
– These proteins assist in facilitated diffusion, a type of passive transport that does not require energy
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▪ Some proteins function by becoming a hydrophilic tunnel for passage
– Other proteins bind their passenger, change shape, and release their passenger on the other side
– In both of these situations, the protein is specific for the substrate, which can be sugars, amino acids, ions, and even water
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Solutemolecule
Transportprotein
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5.7 TALKING ABOUT SCIENCE: Peter Agre talks about aquaporins, water-channel proteins found in some cells
▪ The cell membrane contains hourglass-shaped proteins that are responsible for entry and exit of water through the membrane
– Dr. Peter Agre, a physician at the Johns Hopkins University School of Medicine, discovered these transport proteins and called them aquaporins
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5.8 Cells expend energy in the active transport of a solute against its concentration gradient
▪ Cells have a mechanism for moving a solute against its concentration gradient
– It requires the expenditure of energy in the form of ATP
– The mechanism alters the shape of the membrane protein through phosphorylation using ATP
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Transportprotein
Solute
Solute binding1
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Transportprotein
Solute
Solute binding1 Phosphorylation2
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Transportprotein
Solute
Solute binding1 Phosphorylation2 Transport3
Proteinchanges shape
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Transportprotein
Solute
Solute binding1 Phosphorylation2 Transport3
Proteinchanges shape
Protein reversion
4
Phosphatedetaches
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1. Hand up outlines2. Take out dialysis labs3. Take out notebooks
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5.9 Exocytosis and endocytosis transport large molecules across membranes
▪ A cell uses two mechanisms for moving large molecules across membranes
– Exocytosis is used to export bulky molecules, such as proteins or polysaccharides
– Endocytosis is used to import substances useful to the livelihood of the cell
▪ In both cases, material to be transported is packaged within a vesicle that fuses with the membrane
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▪ There are three kinds of endocytosis– Phagocytosis is engulfment of a particle by wrapping
cell membrane around it, forming a vacuole
– Pinocytosis is the same thing except that fluids are taken into small vesicles
– Receptor-mediated endocytosis is where receptors in a receptor-coated pit interact with a specific protein, initiating formation of a vesicle
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Phagocytosis
EXTRACELLULARFLUID
Pseudopodium
CYTOPLASM
Foodvacuole
“Food” orother particle
Pinocytosis
Plasmamembrane
Vesicle
Coatedvesicle
Coatedpit
Specificmolecule
Receptor-mediated endocytosisCoat protein
Receptor
Coatedpit
Material boundto receptor proteins
Plasma membrane
Foodbeingingested
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Phagocytosis
EXTRACELLULARFLUID
Pseudopodium
CYTOPLASM
Foodvacuole
“Food” orother particle
Foodbeingingested
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Pinocytosis
Plasmamembrane
Vesicle
Plasma membrane
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Coatedvesicle
Coatedpit
Specificmolecule
Receptor-mediated endocytosisCoat protein
Receptor
Coatedpit
Material boundto receptor proteins
Plasma membrane
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ENERGY AND THE CELL
DO NOW:
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5.10 Cells transform energy as they perform work
▪ Cells are small units, a chemical factory, housing thousands of chemical reactions
– The result of reactions is maintenance of the cell, manufacture of cellular parts, and replication
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Energy is the capacity to do work and cause change
Work is accomplished when an object is moved against an opposing force, such as friction
There are two kinds of energy:
1. Kinetic energy is the energy of motion
2. Potential energy is energy that an object possesses as a result of its location
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Kinetic energy performs work by transferring motion to other matter
– EX: water moving through a turbine generates electricity
– Heat, or thermal energy, is kinetic energy associated with the random movement of atoms
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▪ An example of potential energy is water behind a dam
– Chemical energy is potential energy because of its energy available for release in a chemical reaction
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5.11 Two laws govern energy transformations
▪ Energy transformations within matter are studied by individuals in the field of thermodynamics
– Biologists study thermodynamics because an organism exchanges both energy and matter with its surroundings
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▪ It is important to understand two laws that govern energy transformations in organisms
First law of thermodynamics:
energy in the universe is constant
Second law of thermodynamics:
energy conversions increase the disorder of the universe
(Entropy is the measure of disorder, or randomness)
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Fuel
Gasoline
Energy conversion in a cell
Energy for cellular work
Cellular respiration
Waste productsEnergy conversion
Combustion
Energy conversion in a car
Oxygen
Heat
Glucose
Oxygen Water
Carbon dioxide
Water
Carbon dioxide
Kinetic energyof movement
Heatenergy
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Fuel
Gasoline
Waste products
Energy conversion
Combustion
Energy conversion in a car
Oxygen Water
Carbon dioxide
Kinetic energyof movement
Heatenergy
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Energy conversion in a cell
Energy for cellular work
Cellular respiration
Heat
Glucose
Oxygen Water
Carbon dioxide
Fuel Energy conversion Waste products
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5.12 Chemical reactions either release or store energy
▪ An exergonic reaction is a chemical reaction that releases energy
– This reaction releases the energy in covalent bonds of the reactants
– Burning wood releases the energy in glucose, producing heat, light, carbon dioxide, and water
– Cellular respiration also releases energy and heat and produces products but is able to use the released energy to perform work
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Reactants
Amount ofenergy
released
Pote
ntia
l ene
rgy
of m
olec
ules
Energy released
Products
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5.12 Chemical reactions either release or store energy
▪ An endergonic reaction requires an input of energy and yields products rich in potential energy
– The reactants contain little energy in the beginning, but energy is absorbed from the surroundings and stored in covalent bonds of the products
– Photosynthesis makes energy-rich sugar molecules using energy in sunlight
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Reactants
Pote
ntia
l ene
rgy
of m
olec
ules
Energy required
Products
Amount of
energyrequired
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A living organism produces thousands of endergonic and exergonic chemical reactions
● All of these combined is called metabolism
● A metabolic pathway is a series of chemical reactions that either break down a complex molecule or build up a complex molecule
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▪ A cell does three main types of cellular work
– Chemical work—driving endergonic reactions
– Transport work—pumping substances across membranes
– Mechanical work— cilia movement
▪ To accomplish work, a cell must manage its energy resources, and it does so by energy coupling—the use of exergonic processes to drive an endergonic one
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▪ ATP, adenosine triphosphate, is the energy currency of cells.
– ATP is the immediate source of energy that powers most forms of cellular work.
– It is composed of adenine (a nitrogenous base), ribose (a five-carbon sugar), and three phosphate groups.
5.13 ATP shuttles chemical energy and drives cellular work
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▪ Hydrolysis of ATP releases energy by transferring its third phosphate from ATP to some other molecule
– The transfer is called phosphorylation
– In the process, ATP energizes molecules
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Ribose
Adenine
Triphosphate (ATP)
Adenosine
Phosphategroup
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Ribose
Adenine
Triphosphate (ATP)
Adenosine
Phosphategroup
Hydrolysis
Diphosphate (ADP)Adenosine
+
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Chemical work
Solute transported
Molecule formed
Product
Reactants
Motorprotein
Membraneprotein
SoluteTransport workMechanical work
Protein moved
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▪ ATP is a renewable source of energy for the cell
– When energy is released in an exergonic reaction, such as breakdown of glucose, the energy is used in an endergonic reaction to generate ATP
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Energy fromexergonicreactions
Energy forendergonicreactions
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Sodium Potassium PumpSodium (Na+) and Potassium (K+) move against their concentration gradient
by means of active transport. This creates an electrical current.
The outflow of more positive sodium ions than the inflow of positive potassium ions results in a relatively negatively charged cytoplasm (inside of cell is positive and
outside is negative). This is used in neurons and muscles to create the action potentials responsible for nervous system function and muscular contraction.
Going further:- These gradients are used to
propagate electrical signals that travel along nerves.
- Poisons that disable the pump prevent proper functioning of the nervous system.
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action potential - a brief electrical charge that travels down a neuron’s axon. The change in electrical potential associated with the passage of an impulse along the membrane of a muscle cell or nerve cell- Sodium gates open completely during this
Resting potential - an electrical charge difference between the inside and outside of the cell- Sodium channels closed but potassium channels open- Differences actually keep the neuron at “rest”
https://www.youtube.com/watch?v=p0lmlWoEEKY
http://www.slideshare.net/neurosciust/the-resting-potential-and-the-action-potential
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Steps of the Sodium Potassium Pump
1. ATP binds to an active site on the protein forming the Na-K pump, thus providing energy for it.
2. 3 sodium ions (Na+) from the cytoplasm bind to lock and key sites on the Na-K pump.
3. The energized protein of the Na-K pump changes shape, releasing the 3 sodium ions to the extracellular environment.
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4. Two potassium ions (K+) from the extracellular environment, bind to lock and key sites on the protein of the Na-K pump.
5. The protein of the Na-K pump changes shape as the phosphate group leaves the protein's active site.
6. Potassium ions are released to the cytoplasm.
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7. Another ATP molecule binds to the active site on the protein and energizes it.
8. 3 more Na+ ions bind to the membrane protein of the Na-K pump to start the process over.
9. When this process repeats many times, an imbalance of charge forms across the membrane. There will be more positive charged ions outside the membrane than inside. This creates a chemical potential energy which can be used by the cell to later generate lots more ATP, for generating electrical impulses, or for muscle contractions.
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HOW ENZYMES FUNCTION
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Catalase Lab
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5.14 Enzymes speed up the cell’s chemical reactions by lowering energy barriers
▪ Although there is a lot of potential energy in biological molecules, such as carbohydrates and others, it is not released spontaneously
– Energy must be available to break bonds and form new ones
– This energy is called energy of activation (EA)
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The cell uses catalysis to drive (speed up) biological reactions
– Catalysis is accomplished by enzymes, which are proteins that function as biological catalysts
– Enzymes speed up the rate of the reaction by lowering the EA , and they are not used up in the process
– Each enzyme has a particular target molecule called the substrate
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Reactionwithoutenzyme
EA with enzyme
Ener
gy Reactants
Reaction withenzyme
EA withoutenzyme
Netchangein energy(the same)
ProductsProgress of the reaction
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5.15 A specific enzyme catalyzes each cellular reaction
▪ Enzymes have unique three-dimensional shapes
– The shape is critical to their role as biological catalysts
– As a result of its shape, the enzyme has an active site where the enzyme interacts with the enzyme’s substrate
– Consequently, the substrate’s chemistry is altered to form the product of the enzyme reaction
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Enzyme availablewith empty activesite
Active site
1
Enzyme(sucrase)
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Enzyme availablewith empty activesite
Active site
1
Enzyme(sucrase)
Substrate bindsto enzyme withinduced fit
2
Substrate(sucrose)
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Enzyme availablewith empty activesite
Active site
1
Enzyme(sucrase)
Substrate bindsto enzyme withinduced fit
2
Substrate(sucrose)
Substrate isconverted toproducts
3
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Enzyme availablewith empty activesite
Active site
1
Enzyme(sucrase)
Substrate bindsto enzyme withinduced fit
2
Substrate(sucrose)
Substrate isconverted toproducts
3Products arereleased
4
Fructose
Glucose
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▪ For optimum activity, enzymes require certain environmental conditions
– Temperature is very important, and optimally, human enzymes function best at 37ºC, or body temperature
– High temperature will denature human enzymes
– Enzymes also require a pH around neutrality for best results
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▪ Some enzymes require nonprotein helpers
– Cofactors are inorganic, such as zinc, iron, or copper
– Coenzymes are organic molecules and are often vitamins
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▪ Inhibitors are chemicals that inhibit an enzyme’s activity
– One group inhibits because they compete for the enzyme’s active site and thus block substrates from entering the active site
– These are called competitive inhibitors
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Substrate
Enzyme
Active site
Normal binding of substrate
Competitiveinhibitor
Enzyme inhibition
Noncompetitiveinhibitor
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▪ Other inhibitors do not act directly with the active site
– These bind somewhere else and change the shape of the enzyme so that the substrate will no longer fit the active site
– These are called noncompetitive inhibitors
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▪ Enzyme inhibitors are important in regulating cell metabolism
– Often the product of a metabolic pathway can serve as an inhibitor of one enzyme in the pathway, a mechanism called feedback inhibition
– The more product formed, the greater the inhibition, and in this way, regulation of the pathway is accomplished
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Diffusion
Requires no energy
Passive transport
Higher solute concentration
Facilitateddiffusion
OsmosisHigher water
concentration
Higher soluteconcentration
Requires energyActive transport
Solute
Water
Lower soluteconcentration
Lower waterconcentration
Lower soluteconcentration
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ATP cycle
Energy fromexergonicreactions
Energy forendergonicreactions
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Molecules cross
cell membranes
passivetransport
by by
may be movingdown
movingagainst
requires
uses
diffusion
of
polar moleculesand ions
uses
of
(a)
(c)
(d)
(b)
(e)
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a.
b.c.
d.
e.
f.
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Rat
e of
reac
tion
pH109876543210
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1. Describe the cell membrane within the context of the fluid mosaic model
2. Explain how spontaneous formation of a membrane could have been important in the origin of life
3. Describe the passage of materials across a membrane with no energy expenditure
4. Explain how osmosis plays a role in maintenance of a cell
You should now be able to
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5. Explain how an imbalance in water between the cell and its environment affects the cell
6. Describe membrane proteins that facilitate transport of materials across the cell membrane without expenditure of energy
7. Discuss how energy-requiring transport proteins move substances across the cell membrane
8. Distinguish between exocytosis and endocytosis and list similarities between the two
Copyright © 2009 Pearson Education, Inc.
You should now be able to
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9. Explain how energy is transformed during life processes
10. Define the two laws of thermodynamics and explain how they relate to biological systems
11. Explain how a chemical reaction can either release energy or store energy
12. Describe ATP and explain why it is considered to be the energy currency of a cell
Copyright © 2009 Pearson Education, Inc.
You should now be able to
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You should now be able to
13. Define enzyme and explain how enzymes cause a chemical reaction to speed up
14. Discuss the specificity of enzymes
15. Distinguish between competitive inhibitors and noncompetitive inhibitors
Copyright © 2009 Pearson Education, Inc.