The USC San Pedro Ocean Time-series and Microbial …Str 492 Din 568 Alt 555 Hap/Alv 179 S86/Pla 537...
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The USC San Pedro Ocean Time-series and Microbial Observatory Caron Lab: Victoria Campbell, Peter Countway, Alle Lie, *Participants (past and present, alphabetical) and Acknowledgments 0 50 100 150 200 250 300 350 400 450 500 5 8 11 14 17 20 Temperature (ºC) Jan Apr Jul Oct 0 50 100 150 200 250 300 350 400 450 500 0 2 4 6 8 Oxygen (mL L -1 ) Jan Apr Jul Oct 0 25 50 75 100 125 150 0 2 4 6 8 10 Chlorophyll (μg L -1 ) Jan Apr Jul Oct Countway et al. (2010), L&O 4) MICROBIAL DIVERSITY (MO) bacteria, archaea, eukarya and viruses 1) STATION LOCATION and DEPTHS - monthly samples Caron et al. (in prep.) 3) BIOMASS and COUNTS (MO) University of Southern California and the USC Wrigley Institute for Environmental Studies, Los Angeles, CA 90089 Coastal temperate system Oligotrophic to mesotrophic San Pedro Basin (sill depth ~740 m) Maximum depth ~890 m 1998 - current : SPOT program (physical, chemical): 0, 10, 20, 30, 40, 50, 60, 100, 250, 500, 750, 885 m 2000 - current : MO program (diversity, biomass, rates): 5 m, DCM, 150 m, 500 m, 885 m 2) PHYSICAL and CHEMICAL PARAMETERS (SPOT) 5) RATES and FLUXES (MO and ancillary) Collins et al. (2011), DSR-I Continuous profiles of temperature, oxygen and fluorescence from 4 seasons in 2001 (SBE CTD and sensors) Nutrient concentrations from discrete water samples (12 SPOT depths) Monthly averages of MLD, depth of the DCM and chlorophyll-a from 5 m and the DCM (2000-2003) Seasonal variability in temperature and oxygen at MO depths Monthly averages of bacteria and viruses (2000-2011) (SYBR counts) Seasonal myoviral and microbial assemblages: Assemblages from ‘opposite’ seasons (e.g., 6 and 18 months apart) resulted in the lowest average similarity; Annually returning high similarities between assemblages from similar seasons (e.g., 12 and 24 months apart) Chow et al. (in review), ISME Bacteria at 5 m (2000-2010) (ARISA, ITS) Lag (months) Average Bray-Curtis (%) Microbial Association Network Included: Bacteria (circles), Protista (diamonds), and Archaea, (triangles) based on Local Similarity Analysis (rank correlation analysis that allows for time-lagged relationships). Connections show when organisms tend to increase or decrease together over time (solid lines), or conversely when their abundance changes in opposite ways (dashed lines); arrows indicate a correlation lagged by one month. Size of a symbol is proportional to the average relative abundance of that taxon. Sediment trap data & Upwelling Index Participating PIs and labs*: Will Berelson, Doug Capone, David Caron, Jed Fuhrman, Doug Hammond, John Heidelberg, Karla Heidelberg, Fengzhu Sun Poster: Diane Y. Kim ([email protected]) https://dornsife.usc.edu/labs/usc-microbial-observatory http://dornsife.usc.edu/spot/ Kim et al. (in review), ISME Chow and Fuhrman (2012), EM Myoviruses at 5 m (2000-2010) (T-RFLP) Lag (months) Average Bray-Curtis (%) Ecological dynamics of nitrification at 150 m: ammonia oxidizing archaea (AOA) and bacteria (AOB ) (qPCR) Monthly averages of bacterial production at 5 m and the DCM (2000-2011) (Leucine and Thymidine) Chow et al. (in review), ISME Kim et al. (in review), ISME Caron et al. (in prep.) Seasonal biomass of plankton at 5 m (3 year averages, 2000-2003) Haskell et al.(in prep.) Thorium flux was dominated by runoff, as opposed to being attached to organic carbon produced by phytoplankton Fuhrman Lab: J Michael Beman, Cheryl Chow, Jacob Cram, Sun Lab: Li Xia Capone Lab: Troy Gunderson, Robert Hammersley Hammond Lab: William Haskell, Rick Schwartz, Christa Wolfe Berelson Lab: Lisa Collins Wrigley Institute for Environmental Studies: Linda Duguay (WIES Director of research), Troy Gunderson, Ximena Hernandez, We would like to thank the numerous personnel and PIs who have contributed to the success of the SPOT and MO programs throughout the years. We are grateful to the Wrigley Institute for Environmental Studies for providing ship time and techincal expertise. We would also like to thank the captain and crew of the R/V Seawatch and Yellowfin from the Southern California Marine Institute for assisting with sample collection. The MO program was funded by NSF grants MCB-0703159, MCB-0084231 and OCE-1136818. Hammersley et al. (2011), AME Nitrogen fixation rates -highest in surface (mean 5.8 +/- 2.6 umol N m-3 d-1) -dominated by nanoplankton -correlated with SST (r=0.54, p=0.03), with exception of Dec 2005 and Feb 2006 -nitrogen fixation also detected in hypoxic water column at 500 m and 885 m J. Heidelberg Lab: Bill Nelson, Rohan Sachdeva K. Heidelberg Lab: Amy Koid Caron Lab: Steffi Moorthi, Julie Rose, Rebecca Schaffner, Astrid Schnetzer Fuhrman Lab: Ian Hewson, David Needham, Alma Parada, Anand Patel, Catherine Roney, Rohan Sachdeva, Michael Schwalbach, Microbial eukaryotes at 5 m and 150 m (18S rDNA T-RFLP) Kim et al. (in review), ISME Lag (months) Average Bray-Curtis (%) 5 m (2008-2010) 150 m (2000-2003) Jones Lab: Zhihong Zhen Fuhrman Lab: Joshua Steele Wrigley Institute for Environmental Studies: Roberta Marinelli (WIES Director), Michael Neumann, Reni Schimmoeller Los Angeles Los Angeles SPOT/MO 33 33’N, 118 24’W Santa Monica Basin Santa Barbara Basin Catalina Island -Settling velocities 83-125 m/d -POC, PON, bSiO2 correlated with SeaWIFS chl-a (r=0.25-0.28, p<0.01) -highest flux during the spring, but timing varied -upwelling index not lined up with export Beman et al. (2010), EM 2003 2004 2005 2006 Chow et al. (in review), ISME bacteria month month Collins et al. (2011), DSR-I Haskell et al. (in prep.) 2004 2005 2006 2007 0.54 −0.51 −0.49 0.64 0.66 0.52 −0.5 0.55 0.51 0.55 −0.5 0.62 0.55 0.55 0.59 0.49 −0.53 0.5 0.57 −0.56 0.57 0.5 0.49 0.48 0.53 0.55 0.53 −0.52 0.55 0.72 −0.48 0.76 −0.57 0.56 0.59 0.49 0.51 0.51 0.54 −0.51 0.53 0.61 0.54 0.56 0.56 0.73 0.48 0.5 0.51 0.52 −0.47 0.5 −0.5 0.59 0.53 0.55 0.51 0.48 0.5 0.57 0.6 0.54 0.52 0.57 −0.53 Den Alp 678 Str 490 S86 528 Din 126 OTU 60 6 Str 23 8 Alv 272 Str/Art 276 OTU 630 EOTU 344 EOTU 536 EOTU 79 Cil/Cfl 485 Alt 642 OTU 964 EOTU 592 Din 342 EOTU 192 Dia 488 OTU 531 Str 492 Din 568 Alt 555 Hap/Alv 179 S86/Pla 537 EOTU 302 EOTU 482 S11 699 Str 227 Ost 322 Cer/Din 265 S92 759 Syn 1051 EOTU 73 Str/Crc 281 Eury Lin/Alv/Cnd 337 EOTU 319 Ro 949 Str 346 EOTU 599 OTU 474 EOTU 131 Alp/Gam 764 Aca/Tel/Cen 325 Temp S11 719 Bac 594 Fla 854 EOTU 135 Chlor a Chl/Alv 269 S11 675 OTU 513 S92 754 OTU 516 Art 500 Gam 784 Str 279 Rho/Hap/Alv 273 Bac 769 Bac 609 Steele et al. (2011), ISME Google Maps r 2 =0.56 r 2 =0.61 Presented at the International Ocean Carbon Coordination Project (IOCCP) and the Ocean Carbon and Biochemistry Program (OCB) International Time-series Workshop (http://www.whoi.edu/website/TS-workshop/home), November 28-30, 2012 SPOT/MO 33 33’N, 118 24’W
Transcript of The USC San Pedro Ocean Time-series and Microbial …Str 492 Din 568 Alt 555 Hap/Alv 179 S86/Pla 537...
The USC San Pedro Ocean Time-series and Microbial Observatory
Caron Lab: Victoria Campbell, Peter Countway, Alle Lie, *Participants (past and present, alphabetical) and Acknowledgments
0
50
100
150
200
250
300
350
400
450
500
5 8 11 14 17 20
Temperature (ºC)
JanAprJulOct
0
50
100
150
200
250
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500
0 2 4 6 8
Oxygen (mL L-1)
JanAprJulOct
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0 2 4 6 8 10
Chlorophyll (μg L-1)
JanAprJulOct
Countway et al. (2010), L&O
4) MICROBIAL DIVERSITY (MO) bacteria, archaea, eukarya and viruses
1) STATION LOCATION and DEPTHS - monthly samples
Caron et al. (in prep.)
3) BIOMASS and COUNTS (MO)
University of Southern California and the USC Wrigley Institute for Environmental Studies, Los Angeles, CA 90089
Coastal temperate systemOligotrophic to mesotrophic
San Pedro Basin (sill depth ~740 m)Maximum depth ~890 m
1998 - current : SPOT program (physical, chemical): 0, 10, 20, 30, 40, 50, 60, 100, 250, 500, 750, 885 m 2000 - current : MO program (diversity, biomass, rates): 5 m, DCM, 150 m, 500 m, 885 m
2) PHYSICAL and CHEMICAL PARAMETERS (SPOT)
5) RATES and FLUXES (MO and ancillary)
Collins et al. (2011), DSR-I
Continuous profiles of temperature, oxygen and fluorescence from 4 seasons in 2001 (SBE CTD and sensors)
Nutrient concentrations from discrete water samples (12 SPOT depths)Monthly averages of MLD, depth of
the DCM and chlorophyll-a from 5 m and the DCM (2000-2003)
Seasonal variability in temperature and oxygen at MO depths
Monthly averages of bacteria and viruses (2000-2011) (SYBR counts)
Seasonal myoviral and microbial assemblages:Assemblages from ‘opposite’ seasons (e.g., 6 and 18 months apart) resulted in the lowest average similarity;
Annually returning high similarities between assemblages from similar seasons (e.g., 12 and 24 months apart)
Chow et al. (in review), ISME
Bacteria at 5 m (2000-2010) (ARISA, ITS)
Lag (months)
Ave
rag
e B
ray-
Cu
rtis
(%)
Microbial Association Network
Included: Bacteria (circles), Protista (diamonds), and Archaea, (triangles) based on Local Similarity Analysis (rank correlation analysis that allows for time-lagged
relationships). Connections show when organisms tend to increase or decrease together over time (solid lines), or conversely when their abundance changes in
opposite ways (dashed lines); arrows indicate a correlation lagged by one month. Size of a symbol is proportional to the average relative abundance of that taxon.
Sediment trap data& Upwelling Index
Participating PIs and labs*: Will Berelson, Doug Capone, David Caron, Jed Fuhrman, Doug Hammond, John Heidelberg, Karla Heidelberg, Fengzhu SunPoster: Diane Y. Kim ([email protected]) https://dornsife.usc.edu/labs/usc-microbial-observatory http://dornsife.usc.edu/spot/
Kim et al. (in review), ISME
Chow and Fuhrman (2012), EM
Myoviruses at 5 m (2000-2010) (T-RFLP)
Lag (months)
Ave
rag
e B
ray-
Cu
rtis
(%)
Ecological dynamics of nitrification at 150 m: ammonia oxidizing
archaea (AOA) and bacteria (AOB ) (qPCR)
Monthly averages of bacterial production at 5 m and the DCM (2000-2011) (Leucine and Thymidine)
Chow et al. (in review), ISME
Kim et al. (in review), ISME
Caron et al. (in prep.)
Seasonal biomass of plankton at 5 m (3 year averages,
2000-2003)
Haskell et al.(in prep.)
Thorium fluxwas dominated by
runoff, as opposed to being attached to organic carbon produced by phytoplankton
Fuhrman Lab: J Michael Beman, Cheryl Chow, Jacob Cram,
Sun Lab: Li Xia
Capone Lab: Troy Gunderson, Robert Hammersley
Hammond Lab: William Haskell, Rick Schwartz, Christa Wolfe
Berelson Lab: Lisa Collins
Wrigley Institute for Environmental Studies: Linda Duguay (WIES Director of research), Troy Gunderson, Ximena Hernandez,
We would like to thank the numerous personnel and PIs who have contributed to the success of the SPOT and MO programs throughout the years. We are grateful to the Wrigley Institute for Environmental Studies for providing ship time and techincal expertise. We would also like to thank the captain and crew of the R/V Seawatch and Yellowfin from the Southern California Marine Institute for assisting with sample collection. The MO program was funded by NSF grants MCB-0703159, MCB-0084231 and OCE-1136818.
Hammersley et al. (2011), AME
Nitrogen fixation rates
-highest in surface (mean 5.8 +/- 2.6 umol N m-3 d-1)
-dominated by nanoplankton -correlated with SST
(r=0.54, p=0.03), with exception of Dec 2005 and Feb 2006
-nitrogen fixation also detected in hypoxic water column at
500 m and 885 m
J. Heidelberg Lab: Bill Nelson, Rohan Sachdeva K. Heidelberg Lab: Amy Koid
Caron Lab: Steffi Moorthi, Julie Rose, Rebecca Schaffner, Astrid SchnetzerFuhrman Lab: Ian Hewson, David Needham, Alma Parada, Anand Patel, Catherine Roney, Rohan Sachdeva, Michael Schwalbach,
Microbial eukaryotes at 5 m and 150 m (18S rDNA T-RFLP)
Kim et al. (in review), ISMELag (months)
Ave
rag
e B
ray-
Cu
rtis
(%)
5 m (2008-2010) 150 m (2000-2003)
Jones Lab: Zhihong ZhenFuhrman Lab: Joshua Steele
Wrigley Institute for Environmental Studies: Roberta Marinelli (WIES Director), Michael Neumann, Reni Schimmoeller
Los AngelesLos Angeles
SPOT/MO33 33’N, 118 24’W
Santa Monica Basin
Santa Barbara Basin
Catalina Island
-Settling velocities 83-125 m/d
-POC, PON, bSiO2 correlated withSeaWIFS chl-a
(r=0.25-0.28, p<0.01)-highest flux during
the spring, but timing varied
-upwelling index not lined up with export
Beman et al. (2010), EM2003 2004 2005 2006
Chow et al. (in review), ISME
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OTU 60 6
Str 23 8
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Str/Art 276
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EOTU 536
EOTU 79
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Str 227
Ost 322
Cer/Din 265
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Syn 1051
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Str/Crc 281
Eury
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EOTU 319
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EOTU 599
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Alp/Gam 764
Aca/Tel/Cen 325
Temp S11 719
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Google Maps
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