The Role of Histamine in Radiation’ Toxic Effects .
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Dmitri Popov. PhD, Radiobiology. MD (Russia) Advanced Medical Technology and Systems Inc. Canada. The Role of Histamine in Radiation’ Toxic Effects . Part 1.
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Transcript of The Role of Histamine in Radiation’ Toxic Effects .
Dmitri Popov PhD Radiobiology MD (Russia)
Advanced Medical Technology and Systems Inc Canada
The Role of Histamine in Radiationrsquo
Toxic Effects
Part 1
The Role of Histamine in Radiationrsquo Toxic Effects
A biogenic amine is a biogenic substance with one or more amine groups They are basic nitrogenous compounds formed mainly by decarboxylation of amino acids or byamination and transamination of aldehydes andketones Biogenic amines are organic bases with low molecular weight and are synthesized by microbial vegetable and animal metabolisms In food and beverages they are formed by the enzymes of raw material or are generated by microbial decarboxylation of aminoacids httpenwikipediaorgwikiBiogenic_amine
The Role of Histamine in Radiationrsquo Toxic Effects
Classical monoaminesHistamine - a substance
derived from the amino acid histidine that acts as
a neurotransmitter mediating arousal and attention as
well as a pro-inflammatory signal released from mast
cells in response to allergic reactions or tissue
damage Histamine is also an important stimulant of
HCl secretion by the stomach through histamine
H2 receptors
Serotonin - a central nervous system neurotransmitter
derived from the amino acid tryptophan involved in
regulating mood sleep appetite and sexuality
httpenwikipediaorgwikiBiogenic_amine
The Role of Histamine in Radiationrsquo Toxic Effects
Histamine is an organic nitrogenous compound involved in local immune responses as well as regulating physiological function in the gut and acting as a neurotransmitter Histamine is involved in the inflammatory response As part of an immune response to foreign pathogens or physical agents such as radiation histamine is produced by basophils and by mast cells found in nearby connective tissues Histamine increases the permeability of the capillaries to white blood cells and some proteins to allow them to engage pathogens in the infected tissues httpenwikipediaorgwikiBiogenic_amine
The Role of Histamine in Radiationrsquo Toxic Effects
Histamine is derived from the decarboxylation of
the amino acid histidine a reaction catalyzed by
the enzyme L-histidine decarboxylase It is
a hydrophilic vasoactive amine
httpenwikipediaorgwikiHistamine
The Role of Histamine in Radiationrsquo Toxic Effects
Most histamine in the body is generated in granules
in mast cells and in white blood cells
called basophils and eosinophils Mast cells are
especially numerous at sites of potential injury mdash
the nose mouth and feet internal body surfaces
and blood vessels Non-mast cell histamine is
found in several tissues including the brain
where it functions as a neurotransmitter Another
important site of histamine storage and release is
the enterochromaffin-like (ECL) cell of
the stomach
The Role of Histamine in Radiationrsquo Toxic Effects
The most important pathophysiologic mechanism of mast cell and basophil histamine release is immunologic These cells if sensitized by IgE antibodies attached to their membranes degranulate when exposed to the appropriate antigen Certain amines and alkaloids including such drugs as morphine and curare alkaloids can displace histamine in granules and cause its release Antibiotics likepolymyxin are also found to stimulate histamine release RADIATION can also stimulate histamine release
The Role of Histamine in Radiationrsquo Toxic Effects
Histamine release occurs when allergens bind to
mast-cell-bound IgE antibodies Reduction of IgE
overproduction may lower the likelihood of
allergens finding sufficient free IgE to trigger a
mast-cell-release of histamine
How radiation can play a role of allergens and
trigger a mast-cell-release of histamine
The Role of Histamine in Radiationrsquo Toxic Effects
Histamine H1receptor
CNS Produced in the tuberomamillary nucleus
projecting to thedorsal raphe locus coeruleus
and either to andor through thehippocampal
formation amygdala basal
ganglia thalamus superior colliculus
and cerebellum
PNS Smooth muscle and endothelium
The Role of Histamine in Radiationrsquo Toxic Effects
CNS sleep-wake cycle body
temperature nociception endocrine
homeostasisappetite mood learning
and memory
PNS Causes bronchoconstriction
bronchial smooth muscle contractionvasodilation
separation of endothelial cells (responsible
for hives) and pain and itching due to insect
stings the primary receptors involved in allergic
rhinitis symptoms and motion sickness
The Role of Histamine in Radiationrsquo Toxic Effects
Histamine H2receptor
Located on parietal cells and vascular smooth
muscle cells Primarily involved in vasodilation
Also stimulate gastric acid secretion
Histamine H3receptor Found on central nervous
system and to a lesser extent peripheral nervous
system tissue
Decreased neurotransmitter release
histamine acetylcholine norepinephrine
serotonin
The Role of Histamine in Radiationrsquo Toxic Effects
Histamine H4receptor Found primarily in
the basophils and in the bone marrow
It is also found on thymus small
intestine spleen and colon
Plays a role in mast cell chemotaxis
The Role of Histamine in Radiationrsquo Toxic Effects
Tissue mast cells which have been traditionally
studied in the context of post-radiation allergic
inflammation are not well studied and remain
controversial to play a role in activation both innate
and acquired immunity after irradiation
The Role of Histamine in Radiationrsquo Toxic Effects
A qualitative risk assessment of biogenic amines (BA) after irradiation in blood lymph and tissues was conducted using data from the scientific literature as well as original research studies conducted
Histamine and serotonin are considered as the potentially toxic substances after irradiation
Concern due to associated intensive irradiation and enzyme activity and potential for immune system toxic and autoimmune response with auto antibodies and Biogenic Amines formation
The Role of Histamine in Radiationrsquos
Toxic Effects
Scombroid fish poisoning (often called histamine poisoning) is caused by ingestion of certain species of marine fish that contain high levels of histamine and possibly other biogenic amines These fish species contain high levels of free histidine in their tissue and when such fish are subjected to temperature abuse after harvest bacteria decarboxylate histidine to produce histamine A number of bacterial species can produce histamine but Morganellamorganii and Proteus spp are particularly strong histamine producers
The Role of Histamine in Radiationrsquo Toxic Effects
Histamine playing important role in food toxicity
ldquoHistamine is an organic nitrogen compound that may trigger an allergic response in human and mammalian systems Other biogenic amines (such as cadaverine and putrescine) are thought to potentiate the toxic effect of histamine Although fatal cases are rare severity of the symptoms can vary depending on the amount of histamine and other biogenic amines ingested and the individualrsquos sensitivity to specific biogenic aminesrdquo WHO httpwwwwhointfoodsafetyhistamineen
The Role of Histamine in Radiationrsquo Toxic Effects
Scombroid fish poisoning (often called histamine
poisoning) is caused by ingestion of certain species
of marine fish that contain high levels of histamine
and possibly other biogenic amines These fish
species contain high levels of free histidine in their
tissue and when such fish are subjected to
temperature abuse after harvest bacteria
decarboxylate histidine to produce histamine A
number of bacterial species can produce histamine
but Morganella morganii and Proteus spp are
particularly strong histamine producers
httpwwwwhointfoodsafetyhistamineen
The Role of Histamine in Radiationrsquo Toxic Effects
Eating food (fish particularly) with high levels of
histamine can result in Histamine Fish Poisoning
HFP
The symptoms of HFP can be very similar to
severe allergic reactions which can happens
within minutes to hours after ingestion of toxic fish
with high levels of Histamine in tissue
Symptoms of Histamine poisoning include
A burningtingling sensation around of mouth
Rush and or swelling over the chest andor neck
The Role of Histamine in Radiationrsquo Toxic Effects
Lowering of blood pressure
Headache
Itchy skin vomiting diarrhea
Symptoms of irradiation
The prodrome (early symptoms) of Acute Radiation Syndrome typically includes nausea and vomiting headaches fatigue fever and short period of skin reddening These symptoms may occur at radiation doses as low as 35 rad (035 Gy) These symptoms are common to many illnesses (Include HFP) and may not by themselves indicate acute radiation sickness
The Role of Histamine in Radiationrsquo s Toxic Effects The role of Histamine in development of Radiationrsquo s toxic
effects remain very controversial and need to be reconsidered
1 Scientists reported that histamine and biogenic amines playing extremely active role in development patho-physiological consequences after irradiation httpwwwintechopencombookscurrent-topics-in-ionizing-radiation-researchradiation-toxins-molecular-mechanisms-of-toxicity-and-radiomimetic-properties-
Radiation Toxicity A Practical Medical Guide
edited by William Small Gayle E Woloschak
2 Scientists reported that histamine and biogenic amines donrsquot play any role in development patho-physiological consequences and clinical signs after irradiation
The Role of Histamine in Radiationrsquo Toxic Effects 3 Scientists reported that histamine and biogenic amines
playing extremely active role in development Radioprotective mechanisms after irradiation
ldquoHistamine prevents radiation-induced toxicity by increasing proliferation of damaged intestinal mucosa and suppressing apoptosis that was associated with an increase in SOD and Catalase levels This effect might be of clinical value in patients undergoing radiotherapyrdquo Int J Radiat Biol 2007 Oct83(10)653-63
Mechanisms underlying the radioprotective effect of histamine on small intestine
Medina VA1 Croci M Mohamad NA Massari N GarbarinoG Cricco GP Nuacutentildeez MA Martiacuten GA Crescenti EJ BergocRM Rivera ES
The Role of Histamine in Radiationrsquo Toxic Effects Recommended Histamine ELISA Test Kit
Neogenrsquos Histamine ELISA test kit (Life Science Format) is a competitive direct ELISA (Enzyme-Linked ImmunosorbentAssay) in a microwell format that allows users to obtain exact concentrations of histamine in nanograms per milliliter
This kit is designed for in vitro quantification of histamine in various biological fluids by competitive direct enzyme-linked immunosorbent assay (CD-ELISA) This kit is intended for use in investigative research only and not for human clinical diagnostic use
This Histamine ELISA kit (Life Science Format) should not be used for determining histamine levels in scombroid fish Neogenoffers a separate kit for determination of histamine in fish and other foods
Intended User Researchers in biomedical fields
For Research Use Only
The Role of Histamine in Radiationrsquo Toxic Effects
Kit Contents
Histamine Antibody-Coated Microplate (96 well)
Histamine Standards (Ready-to-use)
Histamine Enzyme Conjugate (HRP)
K-Blue Substrate (TMB)
Wash Buffer (25x)
PBS Sample Diluent
Instruction Booklet
The Role of Histamine in Radiationrsquo Toxic Effects
Cross-reactivity
Histamine 10000
Histidine 0008
Cadaverine 0003
Tyramine lt001
Spermine lt001
Putrescine lt001
Trimethylamine lt001
The Role of Histamine in Radiationrsquo Toxic Effects
Specifications
Sensitivity70 BB0 25 ngmL30 BB0 10 ngmL
Assay Range 25 ndash 500 ngmLInter-Assay CV le10 Intra-Assay CV le10 Size 96 well microplateAssay Sample Size 50 μL Total Assay Incubation Time 15 hours Wavelength 650 nm 450 nm with acid stop Storage Conditions 4degC Antibody Monoclonal
The Role of Histamine in Radiationrsquo Toxic Effects
Recommended
For more information please contact us at
Neogen Corporation
Life Science
944 Nandino Boulevard
Lexington KY 40511 USA
800477-8201 (USACanada)
859254-1221
Fax 859255-5532
informneogencom
The Role of Histamine in Radiationrsquo Toxic Effects
A biogenic amine is a biogenic substance with one or more amine groups They are basic nitrogenous compounds formed mainly by decarboxylation of amino acids or byamination and transamination of aldehydes andketones Biogenic amines are organic bases with low molecular weight and are synthesized by microbial vegetable and animal metabolisms In food and beverages they are formed by the enzymes of raw material or are generated by microbial decarboxylation of aminoacids httpenwikipediaorgwikiBiogenic_amine
The Role of Histamine in Radiationrsquo Toxic Effects
Classical monoaminesHistamine - a substance
derived from the amino acid histidine that acts as
a neurotransmitter mediating arousal and attention as
well as a pro-inflammatory signal released from mast
cells in response to allergic reactions or tissue
damage Histamine is also an important stimulant of
HCl secretion by the stomach through histamine
H2 receptors
Serotonin - a central nervous system neurotransmitter
derived from the amino acid tryptophan involved in
regulating mood sleep appetite and sexuality
httpenwikipediaorgwikiBiogenic_amine
The Role of Histamine in Radiationrsquo Toxic Effects
Histamine is an organic nitrogenous compound involved in local immune responses as well as regulating physiological function in the gut and acting as a neurotransmitter Histamine is involved in the inflammatory response As part of an immune response to foreign pathogens or physical agents such as radiation histamine is produced by basophils and by mast cells found in nearby connective tissues Histamine increases the permeability of the capillaries to white blood cells and some proteins to allow them to engage pathogens in the infected tissues httpenwikipediaorgwikiBiogenic_amine
The Role of Histamine in Radiationrsquo Toxic Effects
Histamine is derived from the decarboxylation of
the amino acid histidine a reaction catalyzed by
the enzyme L-histidine decarboxylase It is
a hydrophilic vasoactive amine
httpenwikipediaorgwikiHistamine
The Role of Histamine in Radiationrsquo Toxic Effects
Most histamine in the body is generated in granules
in mast cells and in white blood cells
called basophils and eosinophils Mast cells are
especially numerous at sites of potential injury mdash
the nose mouth and feet internal body surfaces
and blood vessels Non-mast cell histamine is
found in several tissues including the brain
where it functions as a neurotransmitter Another
important site of histamine storage and release is
the enterochromaffin-like (ECL) cell of
the stomach
The Role of Histamine in Radiationrsquo Toxic Effects
The most important pathophysiologic mechanism of mast cell and basophil histamine release is immunologic These cells if sensitized by IgE antibodies attached to their membranes degranulate when exposed to the appropriate antigen Certain amines and alkaloids including such drugs as morphine and curare alkaloids can displace histamine in granules and cause its release Antibiotics likepolymyxin are also found to stimulate histamine release RADIATION can also stimulate histamine release
The Role of Histamine in Radiationrsquo Toxic Effects
Histamine release occurs when allergens bind to
mast-cell-bound IgE antibodies Reduction of IgE
overproduction may lower the likelihood of
allergens finding sufficient free IgE to trigger a
mast-cell-release of histamine
How radiation can play a role of allergens and
trigger a mast-cell-release of histamine
The Role of Histamine in Radiationrsquo Toxic Effects
Histamine H1receptor
CNS Produced in the tuberomamillary nucleus
projecting to thedorsal raphe locus coeruleus
and either to andor through thehippocampal
formation amygdala basal
ganglia thalamus superior colliculus
and cerebellum
PNS Smooth muscle and endothelium
The Role of Histamine in Radiationrsquo Toxic Effects
CNS sleep-wake cycle body
temperature nociception endocrine
homeostasisappetite mood learning
and memory
PNS Causes bronchoconstriction
bronchial smooth muscle contractionvasodilation
separation of endothelial cells (responsible
for hives) and pain and itching due to insect
stings the primary receptors involved in allergic
rhinitis symptoms and motion sickness
The Role of Histamine in Radiationrsquo Toxic Effects
Histamine H2receptor
Located on parietal cells and vascular smooth
muscle cells Primarily involved in vasodilation
Also stimulate gastric acid secretion
Histamine H3receptor Found on central nervous
system and to a lesser extent peripheral nervous
system tissue
Decreased neurotransmitter release
histamine acetylcholine norepinephrine
serotonin
The Role of Histamine in Radiationrsquo Toxic Effects
Histamine H4receptor Found primarily in
the basophils and in the bone marrow
It is also found on thymus small
intestine spleen and colon
Plays a role in mast cell chemotaxis
The Role of Histamine in Radiationrsquo Toxic Effects
Tissue mast cells which have been traditionally
studied in the context of post-radiation allergic
inflammation are not well studied and remain
controversial to play a role in activation both innate
and acquired immunity after irradiation
The Role of Histamine in Radiationrsquo Toxic Effects
A qualitative risk assessment of biogenic amines (BA) after irradiation in blood lymph and tissues was conducted using data from the scientific literature as well as original research studies conducted
Histamine and serotonin are considered as the potentially toxic substances after irradiation
Concern due to associated intensive irradiation and enzyme activity and potential for immune system toxic and autoimmune response with auto antibodies and Biogenic Amines formation
The Role of Histamine in Radiationrsquos
Toxic Effects
Scombroid fish poisoning (often called histamine poisoning) is caused by ingestion of certain species of marine fish that contain high levels of histamine and possibly other biogenic amines These fish species contain high levels of free histidine in their tissue and when such fish are subjected to temperature abuse after harvest bacteria decarboxylate histidine to produce histamine A number of bacterial species can produce histamine but Morganellamorganii and Proteus spp are particularly strong histamine producers
The Role of Histamine in Radiationrsquo Toxic Effects
Histamine playing important role in food toxicity
ldquoHistamine is an organic nitrogen compound that may trigger an allergic response in human and mammalian systems Other biogenic amines (such as cadaverine and putrescine) are thought to potentiate the toxic effect of histamine Although fatal cases are rare severity of the symptoms can vary depending on the amount of histamine and other biogenic amines ingested and the individualrsquos sensitivity to specific biogenic aminesrdquo WHO httpwwwwhointfoodsafetyhistamineen
The Role of Histamine in Radiationrsquo Toxic Effects
Scombroid fish poisoning (often called histamine
poisoning) is caused by ingestion of certain species
of marine fish that contain high levels of histamine
and possibly other biogenic amines These fish
species contain high levels of free histidine in their
tissue and when such fish are subjected to
temperature abuse after harvest bacteria
decarboxylate histidine to produce histamine A
number of bacterial species can produce histamine
but Morganella morganii and Proteus spp are
particularly strong histamine producers
httpwwwwhointfoodsafetyhistamineen
The Role of Histamine in Radiationrsquo Toxic Effects
Eating food (fish particularly) with high levels of
histamine can result in Histamine Fish Poisoning
HFP
The symptoms of HFP can be very similar to
severe allergic reactions which can happens
within minutes to hours after ingestion of toxic fish
with high levels of Histamine in tissue
Symptoms of Histamine poisoning include
A burningtingling sensation around of mouth
Rush and or swelling over the chest andor neck
The Role of Histamine in Radiationrsquo Toxic Effects
Lowering of blood pressure
Headache
Itchy skin vomiting diarrhea
Symptoms of irradiation
The prodrome (early symptoms) of Acute Radiation Syndrome typically includes nausea and vomiting headaches fatigue fever and short period of skin reddening These symptoms may occur at radiation doses as low as 35 rad (035 Gy) These symptoms are common to many illnesses (Include HFP) and may not by themselves indicate acute radiation sickness
The Role of Histamine in Radiationrsquo s Toxic Effects The role of Histamine in development of Radiationrsquo s toxic
effects remain very controversial and need to be reconsidered
1 Scientists reported that histamine and biogenic amines playing extremely active role in development patho-physiological consequences after irradiation httpwwwintechopencombookscurrent-topics-in-ionizing-radiation-researchradiation-toxins-molecular-mechanisms-of-toxicity-and-radiomimetic-properties-
Radiation Toxicity A Practical Medical Guide
edited by William Small Gayle E Woloschak
2 Scientists reported that histamine and biogenic amines donrsquot play any role in development patho-physiological consequences and clinical signs after irradiation
The Role of Histamine in Radiationrsquo Toxic Effects 3 Scientists reported that histamine and biogenic amines
playing extremely active role in development Radioprotective mechanisms after irradiation
ldquoHistamine prevents radiation-induced toxicity by increasing proliferation of damaged intestinal mucosa and suppressing apoptosis that was associated with an increase in SOD and Catalase levels This effect might be of clinical value in patients undergoing radiotherapyrdquo Int J Radiat Biol 2007 Oct83(10)653-63
Mechanisms underlying the radioprotective effect of histamine on small intestine
Medina VA1 Croci M Mohamad NA Massari N GarbarinoG Cricco GP Nuacutentildeez MA Martiacuten GA Crescenti EJ BergocRM Rivera ES
The Role of Histamine in Radiationrsquo Toxic Effects Recommended Histamine ELISA Test Kit
Neogenrsquos Histamine ELISA test kit (Life Science Format) is a competitive direct ELISA (Enzyme-Linked ImmunosorbentAssay) in a microwell format that allows users to obtain exact concentrations of histamine in nanograms per milliliter
This kit is designed for in vitro quantification of histamine in various biological fluids by competitive direct enzyme-linked immunosorbent assay (CD-ELISA) This kit is intended for use in investigative research only and not for human clinical diagnostic use
This Histamine ELISA kit (Life Science Format) should not be used for determining histamine levels in scombroid fish Neogenoffers a separate kit for determination of histamine in fish and other foods
Intended User Researchers in biomedical fields
For Research Use Only
The Role of Histamine in Radiationrsquo Toxic Effects
Kit Contents
Histamine Antibody-Coated Microplate (96 well)
Histamine Standards (Ready-to-use)
Histamine Enzyme Conjugate (HRP)
K-Blue Substrate (TMB)
Wash Buffer (25x)
PBS Sample Diluent
Instruction Booklet
The Role of Histamine in Radiationrsquo Toxic Effects
Cross-reactivity
Histamine 10000
Histidine 0008
Cadaverine 0003
Tyramine lt001
Spermine lt001
Putrescine lt001
Trimethylamine lt001
The Role of Histamine in Radiationrsquo Toxic Effects
Specifications
Sensitivity70 BB0 25 ngmL30 BB0 10 ngmL
Assay Range 25 ndash 500 ngmLInter-Assay CV le10 Intra-Assay CV le10 Size 96 well microplateAssay Sample Size 50 μL Total Assay Incubation Time 15 hours Wavelength 650 nm 450 nm with acid stop Storage Conditions 4degC Antibody Monoclonal
The Role of Histamine in Radiationrsquo Toxic Effects
Recommended
For more information please contact us at
Neogen Corporation
Life Science
944 Nandino Boulevard
Lexington KY 40511 USA
800477-8201 (USACanada)
859254-1221
Fax 859255-5532
informneogencom
The Role of Histamine in Radiationrsquo Toxic Effects
Classical monoaminesHistamine - a substance
derived from the amino acid histidine that acts as
a neurotransmitter mediating arousal and attention as
well as a pro-inflammatory signal released from mast
cells in response to allergic reactions or tissue
damage Histamine is also an important stimulant of
HCl secretion by the stomach through histamine
H2 receptors
Serotonin - a central nervous system neurotransmitter
derived from the amino acid tryptophan involved in
regulating mood sleep appetite and sexuality
httpenwikipediaorgwikiBiogenic_amine
The Role of Histamine in Radiationrsquo Toxic Effects
Histamine is an organic nitrogenous compound involved in local immune responses as well as regulating physiological function in the gut and acting as a neurotransmitter Histamine is involved in the inflammatory response As part of an immune response to foreign pathogens or physical agents such as radiation histamine is produced by basophils and by mast cells found in nearby connective tissues Histamine increases the permeability of the capillaries to white blood cells and some proteins to allow them to engage pathogens in the infected tissues httpenwikipediaorgwikiBiogenic_amine
The Role of Histamine in Radiationrsquo Toxic Effects
Histamine is derived from the decarboxylation of
the amino acid histidine a reaction catalyzed by
the enzyme L-histidine decarboxylase It is
a hydrophilic vasoactive amine
httpenwikipediaorgwikiHistamine
The Role of Histamine in Radiationrsquo Toxic Effects
Most histamine in the body is generated in granules
in mast cells and in white blood cells
called basophils and eosinophils Mast cells are
especially numerous at sites of potential injury mdash
the nose mouth and feet internal body surfaces
and blood vessels Non-mast cell histamine is
found in several tissues including the brain
where it functions as a neurotransmitter Another
important site of histamine storage and release is
the enterochromaffin-like (ECL) cell of
the stomach
The Role of Histamine in Radiationrsquo Toxic Effects
The most important pathophysiologic mechanism of mast cell and basophil histamine release is immunologic These cells if sensitized by IgE antibodies attached to their membranes degranulate when exposed to the appropriate antigen Certain amines and alkaloids including such drugs as morphine and curare alkaloids can displace histamine in granules and cause its release Antibiotics likepolymyxin are also found to stimulate histamine release RADIATION can also stimulate histamine release
The Role of Histamine in Radiationrsquo Toxic Effects
Histamine release occurs when allergens bind to
mast-cell-bound IgE antibodies Reduction of IgE
overproduction may lower the likelihood of
allergens finding sufficient free IgE to trigger a
mast-cell-release of histamine
How radiation can play a role of allergens and
trigger a mast-cell-release of histamine
The Role of Histamine in Radiationrsquo Toxic Effects
Histamine H1receptor
CNS Produced in the tuberomamillary nucleus
projecting to thedorsal raphe locus coeruleus
and either to andor through thehippocampal
formation amygdala basal
ganglia thalamus superior colliculus
and cerebellum
PNS Smooth muscle and endothelium
The Role of Histamine in Radiationrsquo Toxic Effects
CNS sleep-wake cycle body
temperature nociception endocrine
homeostasisappetite mood learning
and memory
PNS Causes bronchoconstriction
bronchial smooth muscle contractionvasodilation
separation of endothelial cells (responsible
for hives) and pain and itching due to insect
stings the primary receptors involved in allergic
rhinitis symptoms and motion sickness
The Role of Histamine in Radiationrsquo Toxic Effects
Histamine H2receptor
Located on parietal cells and vascular smooth
muscle cells Primarily involved in vasodilation
Also stimulate gastric acid secretion
Histamine H3receptor Found on central nervous
system and to a lesser extent peripheral nervous
system tissue
Decreased neurotransmitter release
histamine acetylcholine norepinephrine
serotonin
The Role of Histamine in Radiationrsquo Toxic Effects
Histamine H4receptor Found primarily in
the basophils and in the bone marrow
It is also found on thymus small
intestine spleen and colon
Plays a role in mast cell chemotaxis
The Role of Histamine in Radiationrsquo Toxic Effects
Tissue mast cells which have been traditionally
studied in the context of post-radiation allergic
inflammation are not well studied and remain
controversial to play a role in activation both innate
and acquired immunity after irradiation
The Role of Histamine in Radiationrsquo Toxic Effects
A qualitative risk assessment of biogenic amines (BA) after irradiation in blood lymph and tissues was conducted using data from the scientific literature as well as original research studies conducted
Histamine and serotonin are considered as the potentially toxic substances after irradiation
Concern due to associated intensive irradiation and enzyme activity and potential for immune system toxic and autoimmune response with auto antibodies and Biogenic Amines formation
The Role of Histamine in Radiationrsquos
Toxic Effects
Scombroid fish poisoning (often called histamine poisoning) is caused by ingestion of certain species of marine fish that contain high levels of histamine and possibly other biogenic amines These fish species contain high levels of free histidine in their tissue and when such fish are subjected to temperature abuse after harvest bacteria decarboxylate histidine to produce histamine A number of bacterial species can produce histamine but Morganellamorganii and Proteus spp are particularly strong histamine producers
The Role of Histamine in Radiationrsquo Toxic Effects
Histamine playing important role in food toxicity
ldquoHistamine is an organic nitrogen compound that may trigger an allergic response in human and mammalian systems Other biogenic amines (such as cadaverine and putrescine) are thought to potentiate the toxic effect of histamine Although fatal cases are rare severity of the symptoms can vary depending on the amount of histamine and other biogenic amines ingested and the individualrsquos sensitivity to specific biogenic aminesrdquo WHO httpwwwwhointfoodsafetyhistamineen
The Role of Histamine in Radiationrsquo Toxic Effects
Scombroid fish poisoning (often called histamine
poisoning) is caused by ingestion of certain species
of marine fish that contain high levels of histamine
and possibly other biogenic amines These fish
species contain high levels of free histidine in their
tissue and when such fish are subjected to
temperature abuse after harvest bacteria
decarboxylate histidine to produce histamine A
number of bacterial species can produce histamine
but Morganella morganii and Proteus spp are
particularly strong histamine producers
httpwwwwhointfoodsafetyhistamineen
The Role of Histamine in Radiationrsquo Toxic Effects
Eating food (fish particularly) with high levels of
histamine can result in Histamine Fish Poisoning
HFP
The symptoms of HFP can be very similar to
severe allergic reactions which can happens
within minutes to hours after ingestion of toxic fish
with high levels of Histamine in tissue
Symptoms of Histamine poisoning include
A burningtingling sensation around of mouth
Rush and or swelling over the chest andor neck
The Role of Histamine in Radiationrsquo Toxic Effects
Lowering of blood pressure
Headache
Itchy skin vomiting diarrhea
Symptoms of irradiation
The prodrome (early symptoms) of Acute Radiation Syndrome typically includes nausea and vomiting headaches fatigue fever and short period of skin reddening These symptoms may occur at radiation doses as low as 35 rad (035 Gy) These symptoms are common to many illnesses (Include HFP) and may not by themselves indicate acute radiation sickness
The Role of Histamine in Radiationrsquo s Toxic Effects The role of Histamine in development of Radiationrsquo s toxic
effects remain very controversial and need to be reconsidered
1 Scientists reported that histamine and biogenic amines playing extremely active role in development patho-physiological consequences after irradiation httpwwwintechopencombookscurrent-topics-in-ionizing-radiation-researchradiation-toxins-molecular-mechanisms-of-toxicity-and-radiomimetic-properties-
Radiation Toxicity A Practical Medical Guide
edited by William Small Gayle E Woloschak
2 Scientists reported that histamine and biogenic amines donrsquot play any role in development patho-physiological consequences and clinical signs after irradiation
The Role of Histamine in Radiationrsquo Toxic Effects 3 Scientists reported that histamine and biogenic amines
playing extremely active role in development Radioprotective mechanisms after irradiation
ldquoHistamine prevents radiation-induced toxicity by increasing proliferation of damaged intestinal mucosa and suppressing apoptosis that was associated with an increase in SOD and Catalase levels This effect might be of clinical value in patients undergoing radiotherapyrdquo Int J Radiat Biol 2007 Oct83(10)653-63
Mechanisms underlying the radioprotective effect of histamine on small intestine
Medina VA1 Croci M Mohamad NA Massari N GarbarinoG Cricco GP Nuacutentildeez MA Martiacuten GA Crescenti EJ BergocRM Rivera ES
The Role of Histamine in Radiationrsquo Toxic Effects Recommended Histamine ELISA Test Kit
Neogenrsquos Histamine ELISA test kit (Life Science Format) is a competitive direct ELISA (Enzyme-Linked ImmunosorbentAssay) in a microwell format that allows users to obtain exact concentrations of histamine in nanograms per milliliter
This kit is designed for in vitro quantification of histamine in various biological fluids by competitive direct enzyme-linked immunosorbent assay (CD-ELISA) This kit is intended for use in investigative research only and not for human clinical diagnostic use
This Histamine ELISA kit (Life Science Format) should not be used for determining histamine levels in scombroid fish Neogenoffers a separate kit for determination of histamine in fish and other foods
Intended User Researchers in biomedical fields
For Research Use Only
The Role of Histamine in Radiationrsquo Toxic Effects
Kit Contents
Histamine Antibody-Coated Microplate (96 well)
Histamine Standards (Ready-to-use)
Histamine Enzyme Conjugate (HRP)
K-Blue Substrate (TMB)
Wash Buffer (25x)
PBS Sample Diluent
Instruction Booklet
The Role of Histamine in Radiationrsquo Toxic Effects
Cross-reactivity
Histamine 10000
Histidine 0008
Cadaverine 0003
Tyramine lt001
Spermine lt001
Putrescine lt001
Trimethylamine lt001
The Role of Histamine in Radiationrsquo Toxic Effects
Specifications
Sensitivity70 BB0 25 ngmL30 BB0 10 ngmL
Assay Range 25 ndash 500 ngmLInter-Assay CV le10 Intra-Assay CV le10 Size 96 well microplateAssay Sample Size 50 μL Total Assay Incubation Time 15 hours Wavelength 650 nm 450 nm with acid stop Storage Conditions 4degC Antibody Monoclonal
The Role of Histamine in Radiationrsquo Toxic Effects
Recommended
For more information please contact us at
Neogen Corporation
Life Science
944 Nandino Boulevard
Lexington KY 40511 USA
800477-8201 (USACanada)
859254-1221
Fax 859255-5532
informneogencom
The Role of Histamine in Radiationrsquo Toxic Effects
Histamine is an organic nitrogenous compound involved in local immune responses as well as regulating physiological function in the gut and acting as a neurotransmitter Histamine is involved in the inflammatory response As part of an immune response to foreign pathogens or physical agents such as radiation histamine is produced by basophils and by mast cells found in nearby connective tissues Histamine increases the permeability of the capillaries to white blood cells and some proteins to allow them to engage pathogens in the infected tissues httpenwikipediaorgwikiBiogenic_amine
The Role of Histamine in Radiationrsquo Toxic Effects
Histamine is derived from the decarboxylation of
the amino acid histidine a reaction catalyzed by
the enzyme L-histidine decarboxylase It is
a hydrophilic vasoactive amine
httpenwikipediaorgwikiHistamine
The Role of Histamine in Radiationrsquo Toxic Effects
Most histamine in the body is generated in granules
in mast cells and in white blood cells
called basophils and eosinophils Mast cells are
especially numerous at sites of potential injury mdash
the nose mouth and feet internal body surfaces
and blood vessels Non-mast cell histamine is
found in several tissues including the brain
where it functions as a neurotransmitter Another
important site of histamine storage and release is
the enterochromaffin-like (ECL) cell of
the stomach
The Role of Histamine in Radiationrsquo Toxic Effects
The most important pathophysiologic mechanism of mast cell and basophil histamine release is immunologic These cells if sensitized by IgE antibodies attached to their membranes degranulate when exposed to the appropriate antigen Certain amines and alkaloids including such drugs as morphine and curare alkaloids can displace histamine in granules and cause its release Antibiotics likepolymyxin are also found to stimulate histamine release RADIATION can also stimulate histamine release
The Role of Histamine in Radiationrsquo Toxic Effects
Histamine release occurs when allergens bind to
mast-cell-bound IgE antibodies Reduction of IgE
overproduction may lower the likelihood of
allergens finding sufficient free IgE to trigger a
mast-cell-release of histamine
How radiation can play a role of allergens and
trigger a mast-cell-release of histamine
The Role of Histamine in Radiationrsquo Toxic Effects
Histamine H1receptor
CNS Produced in the tuberomamillary nucleus
projecting to thedorsal raphe locus coeruleus
and either to andor through thehippocampal
formation amygdala basal
ganglia thalamus superior colliculus
and cerebellum
PNS Smooth muscle and endothelium
The Role of Histamine in Radiationrsquo Toxic Effects
CNS sleep-wake cycle body
temperature nociception endocrine
homeostasisappetite mood learning
and memory
PNS Causes bronchoconstriction
bronchial smooth muscle contractionvasodilation
separation of endothelial cells (responsible
for hives) and pain and itching due to insect
stings the primary receptors involved in allergic
rhinitis symptoms and motion sickness
The Role of Histamine in Radiationrsquo Toxic Effects
Histamine H2receptor
Located on parietal cells and vascular smooth
muscle cells Primarily involved in vasodilation
Also stimulate gastric acid secretion
Histamine H3receptor Found on central nervous
system and to a lesser extent peripheral nervous
system tissue
Decreased neurotransmitter release
histamine acetylcholine norepinephrine
serotonin
The Role of Histamine in Radiationrsquo Toxic Effects
Histamine H4receptor Found primarily in
the basophils and in the bone marrow
It is also found on thymus small
intestine spleen and colon
Plays a role in mast cell chemotaxis
The Role of Histamine in Radiationrsquo Toxic Effects
Tissue mast cells which have been traditionally
studied in the context of post-radiation allergic
inflammation are not well studied and remain
controversial to play a role in activation both innate
and acquired immunity after irradiation
The Role of Histamine in Radiationrsquo Toxic Effects
A qualitative risk assessment of biogenic amines (BA) after irradiation in blood lymph and tissues was conducted using data from the scientific literature as well as original research studies conducted
Histamine and serotonin are considered as the potentially toxic substances after irradiation
Concern due to associated intensive irradiation and enzyme activity and potential for immune system toxic and autoimmune response with auto antibodies and Biogenic Amines formation
The Role of Histamine in Radiationrsquos
Toxic Effects
Scombroid fish poisoning (often called histamine poisoning) is caused by ingestion of certain species of marine fish that contain high levels of histamine and possibly other biogenic amines These fish species contain high levels of free histidine in their tissue and when such fish are subjected to temperature abuse after harvest bacteria decarboxylate histidine to produce histamine A number of bacterial species can produce histamine but Morganellamorganii and Proteus spp are particularly strong histamine producers
The Role of Histamine in Radiationrsquo Toxic Effects
Histamine playing important role in food toxicity
ldquoHistamine is an organic nitrogen compound that may trigger an allergic response in human and mammalian systems Other biogenic amines (such as cadaverine and putrescine) are thought to potentiate the toxic effect of histamine Although fatal cases are rare severity of the symptoms can vary depending on the amount of histamine and other biogenic amines ingested and the individualrsquos sensitivity to specific biogenic aminesrdquo WHO httpwwwwhointfoodsafetyhistamineen
The Role of Histamine in Radiationrsquo Toxic Effects
Scombroid fish poisoning (often called histamine
poisoning) is caused by ingestion of certain species
of marine fish that contain high levels of histamine
and possibly other biogenic amines These fish
species contain high levels of free histidine in their
tissue and when such fish are subjected to
temperature abuse after harvest bacteria
decarboxylate histidine to produce histamine A
number of bacterial species can produce histamine
but Morganella morganii and Proteus spp are
particularly strong histamine producers
httpwwwwhointfoodsafetyhistamineen
The Role of Histamine in Radiationrsquo Toxic Effects
Eating food (fish particularly) with high levels of
histamine can result in Histamine Fish Poisoning
HFP
The symptoms of HFP can be very similar to
severe allergic reactions which can happens
within minutes to hours after ingestion of toxic fish
with high levels of Histamine in tissue
Symptoms of Histamine poisoning include
A burningtingling sensation around of mouth
Rush and or swelling over the chest andor neck
The Role of Histamine in Radiationrsquo Toxic Effects
Lowering of blood pressure
Headache
Itchy skin vomiting diarrhea
Symptoms of irradiation
The prodrome (early symptoms) of Acute Radiation Syndrome typically includes nausea and vomiting headaches fatigue fever and short period of skin reddening These symptoms may occur at radiation doses as low as 35 rad (035 Gy) These symptoms are common to many illnesses (Include HFP) and may not by themselves indicate acute radiation sickness
The Role of Histamine in Radiationrsquo s Toxic Effects The role of Histamine in development of Radiationrsquo s toxic
effects remain very controversial and need to be reconsidered
1 Scientists reported that histamine and biogenic amines playing extremely active role in development patho-physiological consequences after irradiation httpwwwintechopencombookscurrent-topics-in-ionizing-radiation-researchradiation-toxins-molecular-mechanisms-of-toxicity-and-radiomimetic-properties-
Radiation Toxicity A Practical Medical Guide
edited by William Small Gayle E Woloschak
2 Scientists reported that histamine and biogenic amines donrsquot play any role in development patho-physiological consequences and clinical signs after irradiation
The Role of Histamine in Radiationrsquo Toxic Effects 3 Scientists reported that histamine and biogenic amines
playing extremely active role in development Radioprotective mechanisms after irradiation
ldquoHistamine prevents radiation-induced toxicity by increasing proliferation of damaged intestinal mucosa and suppressing apoptosis that was associated with an increase in SOD and Catalase levels This effect might be of clinical value in patients undergoing radiotherapyrdquo Int J Radiat Biol 2007 Oct83(10)653-63
Mechanisms underlying the radioprotective effect of histamine on small intestine
Medina VA1 Croci M Mohamad NA Massari N GarbarinoG Cricco GP Nuacutentildeez MA Martiacuten GA Crescenti EJ BergocRM Rivera ES
The Role of Histamine in Radiationrsquo Toxic Effects Recommended Histamine ELISA Test Kit
Neogenrsquos Histamine ELISA test kit (Life Science Format) is a competitive direct ELISA (Enzyme-Linked ImmunosorbentAssay) in a microwell format that allows users to obtain exact concentrations of histamine in nanograms per milliliter
This kit is designed for in vitro quantification of histamine in various biological fluids by competitive direct enzyme-linked immunosorbent assay (CD-ELISA) This kit is intended for use in investigative research only and not for human clinical diagnostic use
This Histamine ELISA kit (Life Science Format) should not be used for determining histamine levels in scombroid fish Neogenoffers a separate kit for determination of histamine in fish and other foods
Intended User Researchers in biomedical fields
For Research Use Only
The Role of Histamine in Radiationrsquo Toxic Effects
Kit Contents
Histamine Antibody-Coated Microplate (96 well)
Histamine Standards (Ready-to-use)
Histamine Enzyme Conjugate (HRP)
K-Blue Substrate (TMB)
Wash Buffer (25x)
PBS Sample Diluent
Instruction Booklet
The Role of Histamine in Radiationrsquo Toxic Effects
Cross-reactivity
Histamine 10000
Histidine 0008
Cadaverine 0003
Tyramine lt001
Spermine lt001
Putrescine lt001
Trimethylamine lt001
The Role of Histamine in Radiationrsquo Toxic Effects
Specifications
Sensitivity70 BB0 25 ngmL30 BB0 10 ngmL
Assay Range 25 ndash 500 ngmLInter-Assay CV le10 Intra-Assay CV le10 Size 96 well microplateAssay Sample Size 50 μL Total Assay Incubation Time 15 hours Wavelength 650 nm 450 nm with acid stop Storage Conditions 4degC Antibody Monoclonal
The Role of Histamine in Radiationrsquo Toxic Effects
Recommended
For more information please contact us at
Neogen Corporation
Life Science
944 Nandino Boulevard
Lexington KY 40511 USA
800477-8201 (USACanada)
859254-1221
Fax 859255-5532
informneogencom
The Role of Histamine in Radiationrsquo Toxic Effects
Histamine is derived from the decarboxylation of
the amino acid histidine a reaction catalyzed by
the enzyme L-histidine decarboxylase It is
a hydrophilic vasoactive amine
httpenwikipediaorgwikiHistamine
The Role of Histamine in Radiationrsquo Toxic Effects
Most histamine in the body is generated in granules
in mast cells and in white blood cells
called basophils and eosinophils Mast cells are
especially numerous at sites of potential injury mdash
the nose mouth and feet internal body surfaces
and blood vessels Non-mast cell histamine is
found in several tissues including the brain
where it functions as a neurotransmitter Another
important site of histamine storage and release is
the enterochromaffin-like (ECL) cell of
the stomach
The Role of Histamine in Radiationrsquo Toxic Effects
The most important pathophysiologic mechanism of mast cell and basophil histamine release is immunologic These cells if sensitized by IgE antibodies attached to their membranes degranulate when exposed to the appropriate antigen Certain amines and alkaloids including such drugs as morphine and curare alkaloids can displace histamine in granules and cause its release Antibiotics likepolymyxin are also found to stimulate histamine release RADIATION can also stimulate histamine release
The Role of Histamine in Radiationrsquo Toxic Effects
Histamine release occurs when allergens bind to
mast-cell-bound IgE antibodies Reduction of IgE
overproduction may lower the likelihood of
allergens finding sufficient free IgE to trigger a
mast-cell-release of histamine
How radiation can play a role of allergens and
trigger a mast-cell-release of histamine
The Role of Histamine in Radiationrsquo Toxic Effects
Histamine H1receptor
CNS Produced in the tuberomamillary nucleus
projecting to thedorsal raphe locus coeruleus
and either to andor through thehippocampal
formation amygdala basal
ganglia thalamus superior colliculus
and cerebellum
PNS Smooth muscle and endothelium
The Role of Histamine in Radiationrsquo Toxic Effects
CNS sleep-wake cycle body
temperature nociception endocrine
homeostasisappetite mood learning
and memory
PNS Causes bronchoconstriction
bronchial smooth muscle contractionvasodilation
separation of endothelial cells (responsible
for hives) and pain and itching due to insect
stings the primary receptors involved in allergic
rhinitis symptoms and motion sickness
The Role of Histamine in Radiationrsquo Toxic Effects
Histamine H2receptor
Located on parietal cells and vascular smooth
muscle cells Primarily involved in vasodilation
Also stimulate gastric acid secretion
Histamine H3receptor Found on central nervous
system and to a lesser extent peripheral nervous
system tissue
Decreased neurotransmitter release
histamine acetylcholine norepinephrine
serotonin
The Role of Histamine in Radiationrsquo Toxic Effects
Histamine H4receptor Found primarily in
the basophils and in the bone marrow
It is also found on thymus small
intestine spleen and colon
Plays a role in mast cell chemotaxis
The Role of Histamine in Radiationrsquo Toxic Effects
Tissue mast cells which have been traditionally
studied in the context of post-radiation allergic
inflammation are not well studied and remain
controversial to play a role in activation both innate
and acquired immunity after irradiation
The Role of Histamine in Radiationrsquo Toxic Effects
A qualitative risk assessment of biogenic amines (BA) after irradiation in blood lymph and tissues was conducted using data from the scientific literature as well as original research studies conducted
Histamine and serotonin are considered as the potentially toxic substances after irradiation
Concern due to associated intensive irradiation and enzyme activity and potential for immune system toxic and autoimmune response with auto antibodies and Biogenic Amines formation
The Role of Histamine in Radiationrsquos
Toxic Effects
Scombroid fish poisoning (often called histamine poisoning) is caused by ingestion of certain species of marine fish that contain high levels of histamine and possibly other biogenic amines These fish species contain high levels of free histidine in their tissue and when such fish are subjected to temperature abuse after harvest bacteria decarboxylate histidine to produce histamine A number of bacterial species can produce histamine but Morganellamorganii and Proteus spp are particularly strong histamine producers
The Role of Histamine in Radiationrsquo Toxic Effects
Histamine playing important role in food toxicity
ldquoHistamine is an organic nitrogen compound that may trigger an allergic response in human and mammalian systems Other biogenic amines (such as cadaverine and putrescine) are thought to potentiate the toxic effect of histamine Although fatal cases are rare severity of the symptoms can vary depending on the amount of histamine and other biogenic amines ingested and the individualrsquos sensitivity to specific biogenic aminesrdquo WHO httpwwwwhointfoodsafetyhistamineen
The Role of Histamine in Radiationrsquo Toxic Effects
Scombroid fish poisoning (often called histamine
poisoning) is caused by ingestion of certain species
of marine fish that contain high levels of histamine
and possibly other biogenic amines These fish
species contain high levels of free histidine in their
tissue and when such fish are subjected to
temperature abuse after harvest bacteria
decarboxylate histidine to produce histamine A
number of bacterial species can produce histamine
but Morganella morganii and Proteus spp are
particularly strong histamine producers
httpwwwwhointfoodsafetyhistamineen
The Role of Histamine in Radiationrsquo Toxic Effects
Eating food (fish particularly) with high levels of
histamine can result in Histamine Fish Poisoning
HFP
The symptoms of HFP can be very similar to
severe allergic reactions which can happens
within minutes to hours after ingestion of toxic fish
with high levels of Histamine in tissue
Symptoms of Histamine poisoning include
A burningtingling sensation around of mouth
Rush and or swelling over the chest andor neck
The Role of Histamine in Radiationrsquo Toxic Effects
Lowering of blood pressure
Headache
Itchy skin vomiting diarrhea
Symptoms of irradiation
The prodrome (early symptoms) of Acute Radiation Syndrome typically includes nausea and vomiting headaches fatigue fever and short period of skin reddening These symptoms may occur at radiation doses as low as 35 rad (035 Gy) These symptoms are common to many illnesses (Include HFP) and may not by themselves indicate acute radiation sickness
The Role of Histamine in Radiationrsquo s Toxic Effects The role of Histamine in development of Radiationrsquo s toxic
effects remain very controversial and need to be reconsidered
1 Scientists reported that histamine and biogenic amines playing extremely active role in development patho-physiological consequences after irradiation httpwwwintechopencombookscurrent-topics-in-ionizing-radiation-researchradiation-toxins-molecular-mechanisms-of-toxicity-and-radiomimetic-properties-
Radiation Toxicity A Practical Medical Guide
edited by William Small Gayle E Woloschak
2 Scientists reported that histamine and biogenic amines donrsquot play any role in development patho-physiological consequences and clinical signs after irradiation
The Role of Histamine in Radiationrsquo Toxic Effects 3 Scientists reported that histamine and biogenic amines
playing extremely active role in development Radioprotective mechanisms after irradiation
ldquoHistamine prevents radiation-induced toxicity by increasing proliferation of damaged intestinal mucosa and suppressing apoptosis that was associated with an increase in SOD and Catalase levels This effect might be of clinical value in patients undergoing radiotherapyrdquo Int J Radiat Biol 2007 Oct83(10)653-63
Mechanisms underlying the radioprotective effect of histamine on small intestine
Medina VA1 Croci M Mohamad NA Massari N GarbarinoG Cricco GP Nuacutentildeez MA Martiacuten GA Crescenti EJ BergocRM Rivera ES
The Role of Histamine in Radiationrsquo Toxic Effects Recommended Histamine ELISA Test Kit
Neogenrsquos Histamine ELISA test kit (Life Science Format) is a competitive direct ELISA (Enzyme-Linked ImmunosorbentAssay) in a microwell format that allows users to obtain exact concentrations of histamine in nanograms per milliliter
This kit is designed for in vitro quantification of histamine in various biological fluids by competitive direct enzyme-linked immunosorbent assay (CD-ELISA) This kit is intended for use in investigative research only and not for human clinical diagnostic use
This Histamine ELISA kit (Life Science Format) should not be used for determining histamine levels in scombroid fish Neogenoffers a separate kit for determination of histamine in fish and other foods
Intended User Researchers in biomedical fields
For Research Use Only
The Role of Histamine in Radiationrsquo Toxic Effects
Kit Contents
Histamine Antibody-Coated Microplate (96 well)
Histamine Standards (Ready-to-use)
Histamine Enzyme Conjugate (HRP)
K-Blue Substrate (TMB)
Wash Buffer (25x)
PBS Sample Diluent
Instruction Booklet
The Role of Histamine in Radiationrsquo Toxic Effects
Cross-reactivity
Histamine 10000
Histidine 0008
Cadaverine 0003
Tyramine lt001
Spermine lt001
Putrescine lt001
Trimethylamine lt001
The Role of Histamine in Radiationrsquo Toxic Effects
Specifications
Sensitivity70 BB0 25 ngmL30 BB0 10 ngmL
Assay Range 25 ndash 500 ngmLInter-Assay CV le10 Intra-Assay CV le10 Size 96 well microplateAssay Sample Size 50 μL Total Assay Incubation Time 15 hours Wavelength 650 nm 450 nm with acid stop Storage Conditions 4degC Antibody Monoclonal
The Role of Histamine in Radiationrsquo Toxic Effects
Recommended
For more information please contact us at
Neogen Corporation
Life Science
944 Nandino Boulevard
Lexington KY 40511 USA
800477-8201 (USACanada)
859254-1221
Fax 859255-5532
informneogencom
The Role of Histamine in Radiationrsquo Toxic Effects
Most histamine in the body is generated in granules
in mast cells and in white blood cells
called basophils and eosinophils Mast cells are
especially numerous at sites of potential injury mdash
the nose mouth and feet internal body surfaces
and blood vessels Non-mast cell histamine is
found in several tissues including the brain
where it functions as a neurotransmitter Another
important site of histamine storage and release is
the enterochromaffin-like (ECL) cell of
the stomach
The Role of Histamine in Radiationrsquo Toxic Effects
The most important pathophysiologic mechanism of mast cell and basophil histamine release is immunologic These cells if sensitized by IgE antibodies attached to their membranes degranulate when exposed to the appropriate antigen Certain amines and alkaloids including such drugs as morphine and curare alkaloids can displace histamine in granules and cause its release Antibiotics likepolymyxin are also found to stimulate histamine release RADIATION can also stimulate histamine release
The Role of Histamine in Radiationrsquo Toxic Effects
Histamine release occurs when allergens bind to
mast-cell-bound IgE antibodies Reduction of IgE
overproduction may lower the likelihood of
allergens finding sufficient free IgE to trigger a
mast-cell-release of histamine
How radiation can play a role of allergens and
trigger a mast-cell-release of histamine
The Role of Histamine in Radiationrsquo Toxic Effects
Histamine H1receptor
CNS Produced in the tuberomamillary nucleus
projecting to thedorsal raphe locus coeruleus
and either to andor through thehippocampal
formation amygdala basal
ganglia thalamus superior colliculus
and cerebellum
PNS Smooth muscle and endothelium
The Role of Histamine in Radiationrsquo Toxic Effects
CNS sleep-wake cycle body
temperature nociception endocrine
homeostasisappetite mood learning
and memory
PNS Causes bronchoconstriction
bronchial smooth muscle contractionvasodilation
separation of endothelial cells (responsible
for hives) and pain and itching due to insect
stings the primary receptors involved in allergic
rhinitis symptoms and motion sickness
The Role of Histamine in Radiationrsquo Toxic Effects
Histamine H2receptor
Located on parietal cells and vascular smooth
muscle cells Primarily involved in vasodilation
Also stimulate gastric acid secretion
Histamine H3receptor Found on central nervous
system and to a lesser extent peripheral nervous
system tissue
Decreased neurotransmitter release
histamine acetylcholine norepinephrine
serotonin
The Role of Histamine in Radiationrsquo Toxic Effects
Histamine H4receptor Found primarily in
the basophils and in the bone marrow
It is also found on thymus small
intestine spleen and colon
Plays a role in mast cell chemotaxis
The Role of Histamine in Radiationrsquo Toxic Effects
Tissue mast cells which have been traditionally
studied in the context of post-radiation allergic
inflammation are not well studied and remain
controversial to play a role in activation both innate
and acquired immunity after irradiation
The Role of Histamine in Radiationrsquo Toxic Effects
A qualitative risk assessment of biogenic amines (BA) after irradiation in blood lymph and tissues was conducted using data from the scientific literature as well as original research studies conducted
Histamine and serotonin are considered as the potentially toxic substances after irradiation
Concern due to associated intensive irradiation and enzyme activity and potential for immune system toxic and autoimmune response with auto antibodies and Biogenic Amines formation
The Role of Histamine in Radiationrsquos
Toxic Effects
Scombroid fish poisoning (often called histamine poisoning) is caused by ingestion of certain species of marine fish that contain high levels of histamine and possibly other biogenic amines These fish species contain high levels of free histidine in their tissue and when such fish are subjected to temperature abuse after harvest bacteria decarboxylate histidine to produce histamine A number of bacterial species can produce histamine but Morganellamorganii and Proteus spp are particularly strong histamine producers
The Role of Histamine in Radiationrsquo Toxic Effects
Histamine playing important role in food toxicity
ldquoHistamine is an organic nitrogen compound that may trigger an allergic response in human and mammalian systems Other biogenic amines (such as cadaverine and putrescine) are thought to potentiate the toxic effect of histamine Although fatal cases are rare severity of the symptoms can vary depending on the amount of histamine and other biogenic amines ingested and the individualrsquos sensitivity to specific biogenic aminesrdquo WHO httpwwwwhointfoodsafetyhistamineen
The Role of Histamine in Radiationrsquo Toxic Effects
Scombroid fish poisoning (often called histamine
poisoning) is caused by ingestion of certain species
of marine fish that contain high levels of histamine
and possibly other biogenic amines These fish
species contain high levels of free histidine in their
tissue and when such fish are subjected to
temperature abuse after harvest bacteria
decarboxylate histidine to produce histamine A
number of bacterial species can produce histamine
but Morganella morganii and Proteus spp are
particularly strong histamine producers
httpwwwwhointfoodsafetyhistamineen
The Role of Histamine in Radiationrsquo Toxic Effects
Eating food (fish particularly) with high levels of
histamine can result in Histamine Fish Poisoning
HFP
The symptoms of HFP can be very similar to
severe allergic reactions which can happens
within minutes to hours after ingestion of toxic fish
with high levels of Histamine in tissue
Symptoms of Histamine poisoning include
A burningtingling sensation around of mouth
Rush and or swelling over the chest andor neck
The Role of Histamine in Radiationrsquo Toxic Effects
Lowering of blood pressure
Headache
Itchy skin vomiting diarrhea
Symptoms of irradiation
The prodrome (early symptoms) of Acute Radiation Syndrome typically includes nausea and vomiting headaches fatigue fever and short period of skin reddening These symptoms may occur at radiation doses as low as 35 rad (035 Gy) These symptoms are common to many illnesses (Include HFP) and may not by themselves indicate acute radiation sickness
The Role of Histamine in Radiationrsquo s Toxic Effects The role of Histamine in development of Radiationrsquo s toxic
effects remain very controversial and need to be reconsidered
1 Scientists reported that histamine and biogenic amines playing extremely active role in development patho-physiological consequences after irradiation httpwwwintechopencombookscurrent-topics-in-ionizing-radiation-researchradiation-toxins-molecular-mechanisms-of-toxicity-and-radiomimetic-properties-
Radiation Toxicity A Practical Medical Guide
edited by William Small Gayle E Woloschak
2 Scientists reported that histamine and biogenic amines donrsquot play any role in development patho-physiological consequences and clinical signs after irradiation
The Role of Histamine in Radiationrsquo Toxic Effects 3 Scientists reported that histamine and biogenic amines
playing extremely active role in development Radioprotective mechanisms after irradiation
ldquoHistamine prevents radiation-induced toxicity by increasing proliferation of damaged intestinal mucosa and suppressing apoptosis that was associated with an increase in SOD and Catalase levels This effect might be of clinical value in patients undergoing radiotherapyrdquo Int J Radiat Biol 2007 Oct83(10)653-63
Mechanisms underlying the radioprotective effect of histamine on small intestine
Medina VA1 Croci M Mohamad NA Massari N GarbarinoG Cricco GP Nuacutentildeez MA Martiacuten GA Crescenti EJ BergocRM Rivera ES
The Role of Histamine in Radiationrsquo Toxic Effects Recommended Histamine ELISA Test Kit
Neogenrsquos Histamine ELISA test kit (Life Science Format) is a competitive direct ELISA (Enzyme-Linked ImmunosorbentAssay) in a microwell format that allows users to obtain exact concentrations of histamine in nanograms per milliliter
This kit is designed for in vitro quantification of histamine in various biological fluids by competitive direct enzyme-linked immunosorbent assay (CD-ELISA) This kit is intended for use in investigative research only and not for human clinical diagnostic use
This Histamine ELISA kit (Life Science Format) should not be used for determining histamine levels in scombroid fish Neogenoffers a separate kit for determination of histamine in fish and other foods
Intended User Researchers in biomedical fields
For Research Use Only
The Role of Histamine in Radiationrsquo Toxic Effects
Kit Contents
Histamine Antibody-Coated Microplate (96 well)
Histamine Standards (Ready-to-use)
Histamine Enzyme Conjugate (HRP)
K-Blue Substrate (TMB)
Wash Buffer (25x)
PBS Sample Diluent
Instruction Booklet
The Role of Histamine in Radiationrsquo Toxic Effects
Cross-reactivity
Histamine 10000
Histidine 0008
Cadaverine 0003
Tyramine lt001
Spermine lt001
Putrescine lt001
Trimethylamine lt001
The Role of Histamine in Radiationrsquo Toxic Effects
Specifications
Sensitivity70 BB0 25 ngmL30 BB0 10 ngmL
Assay Range 25 ndash 500 ngmLInter-Assay CV le10 Intra-Assay CV le10 Size 96 well microplateAssay Sample Size 50 μL Total Assay Incubation Time 15 hours Wavelength 650 nm 450 nm with acid stop Storage Conditions 4degC Antibody Monoclonal
The Role of Histamine in Radiationrsquo Toxic Effects
Recommended
For more information please contact us at
Neogen Corporation
Life Science
944 Nandino Boulevard
Lexington KY 40511 USA
800477-8201 (USACanada)
859254-1221
Fax 859255-5532
informneogencom
The Role of Histamine in Radiationrsquo Toxic Effects
The most important pathophysiologic mechanism of mast cell and basophil histamine release is immunologic These cells if sensitized by IgE antibodies attached to their membranes degranulate when exposed to the appropriate antigen Certain amines and alkaloids including such drugs as morphine and curare alkaloids can displace histamine in granules and cause its release Antibiotics likepolymyxin are also found to stimulate histamine release RADIATION can also stimulate histamine release
The Role of Histamine in Radiationrsquo Toxic Effects
Histamine release occurs when allergens bind to
mast-cell-bound IgE antibodies Reduction of IgE
overproduction may lower the likelihood of
allergens finding sufficient free IgE to trigger a
mast-cell-release of histamine
How radiation can play a role of allergens and
trigger a mast-cell-release of histamine
The Role of Histamine in Radiationrsquo Toxic Effects
Histamine H1receptor
CNS Produced in the tuberomamillary nucleus
projecting to thedorsal raphe locus coeruleus
and either to andor through thehippocampal
formation amygdala basal
ganglia thalamus superior colliculus
and cerebellum
PNS Smooth muscle and endothelium
The Role of Histamine in Radiationrsquo Toxic Effects
CNS sleep-wake cycle body
temperature nociception endocrine
homeostasisappetite mood learning
and memory
PNS Causes bronchoconstriction
bronchial smooth muscle contractionvasodilation
separation of endothelial cells (responsible
for hives) and pain and itching due to insect
stings the primary receptors involved in allergic
rhinitis symptoms and motion sickness
The Role of Histamine in Radiationrsquo Toxic Effects
Histamine H2receptor
Located on parietal cells and vascular smooth
muscle cells Primarily involved in vasodilation
Also stimulate gastric acid secretion
Histamine H3receptor Found on central nervous
system and to a lesser extent peripheral nervous
system tissue
Decreased neurotransmitter release
histamine acetylcholine norepinephrine
serotonin
The Role of Histamine in Radiationrsquo Toxic Effects
Histamine H4receptor Found primarily in
the basophils and in the bone marrow
It is also found on thymus small
intestine spleen and colon
Plays a role in mast cell chemotaxis
The Role of Histamine in Radiationrsquo Toxic Effects
Tissue mast cells which have been traditionally
studied in the context of post-radiation allergic
inflammation are not well studied and remain
controversial to play a role in activation both innate
and acquired immunity after irradiation
The Role of Histamine in Radiationrsquo Toxic Effects
A qualitative risk assessment of biogenic amines (BA) after irradiation in blood lymph and tissues was conducted using data from the scientific literature as well as original research studies conducted
Histamine and serotonin are considered as the potentially toxic substances after irradiation
Concern due to associated intensive irradiation and enzyme activity and potential for immune system toxic and autoimmune response with auto antibodies and Biogenic Amines formation
The Role of Histamine in Radiationrsquos
Toxic Effects
Scombroid fish poisoning (often called histamine poisoning) is caused by ingestion of certain species of marine fish that contain high levels of histamine and possibly other biogenic amines These fish species contain high levels of free histidine in their tissue and when such fish are subjected to temperature abuse after harvest bacteria decarboxylate histidine to produce histamine A number of bacterial species can produce histamine but Morganellamorganii and Proteus spp are particularly strong histamine producers
The Role of Histamine in Radiationrsquo Toxic Effects
Histamine playing important role in food toxicity
ldquoHistamine is an organic nitrogen compound that may trigger an allergic response in human and mammalian systems Other biogenic amines (such as cadaverine and putrescine) are thought to potentiate the toxic effect of histamine Although fatal cases are rare severity of the symptoms can vary depending on the amount of histamine and other biogenic amines ingested and the individualrsquos sensitivity to specific biogenic aminesrdquo WHO httpwwwwhointfoodsafetyhistamineen
The Role of Histamine in Radiationrsquo Toxic Effects
Scombroid fish poisoning (often called histamine
poisoning) is caused by ingestion of certain species
of marine fish that contain high levels of histamine
and possibly other biogenic amines These fish
species contain high levels of free histidine in their
tissue and when such fish are subjected to
temperature abuse after harvest bacteria
decarboxylate histidine to produce histamine A
number of bacterial species can produce histamine
but Morganella morganii and Proteus spp are
particularly strong histamine producers
httpwwwwhointfoodsafetyhistamineen
The Role of Histamine in Radiationrsquo Toxic Effects
Eating food (fish particularly) with high levels of
histamine can result in Histamine Fish Poisoning
HFP
The symptoms of HFP can be very similar to
severe allergic reactions which can happens
within minutes to hours after ingestion of toxic fish
with high levels of Histamine in tissue
Symptoms of Histamine poisoning include
A burningtingling sensation around of mouth
Rush and or swelling over the chest andor neck
The Role of Histamine in Radiationrsquo Toxic Effects
Lowering of blood pressure
Headache
Itchy skin vomiting diarrhea
Symptoms of irradiation
The prodrome (early symptoms) of Acute Radiation Syndrome typically includes nausea and vomiting headaches fatigue fever and short period of skin reddening These symptoms may occur at radiation doses as low as 35 rad (035 Gy) These symptoms are common to many illnesses (Include HFP) and may not by themselves indicate acute radiation sickness
The Role of Histamine in Radiationrsquo s Toxic Effects The role of Histamine in development of Radiationrsquo s toxic
effects remain very controversial and need to be reconsidered
1 Scientists reported that histamine and biogenic amines playing extremely active role in development patho-physiological consequences after irradiation httpwwwintechopencombookscurrent-topics-in-ionizing-radiation-researchradiation-toxins-molecular-mechanisms-of-toxicity-and-radiomimetic-properties-
Radiation Toxicity A Practical Medical Guide
edited by William Small Gayle E Woloschak
2 Scientists reported that histamine and biogenic amines donrsquot play any role in development patho-physiological consequences and clinical signs after irradiation
The Role of Histamine in Radiationrsquo Toxic Effects 3 Scientists reported that histamine and biogenic amines
playing extremely active role in development Radioprotective mechanisms after irradiation
ldquoHistamine prevents radiation-induced toxicity by increasing proliferation of damaged intestinal mucosa and suppressing apoptosis that was associated with an increase in SOD and Catalase levels This effect might be of clinical value in patients undergoing radiotherapyrdquo Int J Radiat Biol 2007 Oct83(10)653-63
Mechanisms underlying the radioprotective effect of histamine on small intestine
Medina VA1 Croci M Mohamad NA Massari N GarbarinoG Cricco GP Nuacutentildeez MA Martiacuten GA Crescenti EJ BergocRM Rivera ES
The Role of Histamine in Radiationrsquo Toxic Effects Recommended Histamine ELISA Test Kit
Neogenrsquos Histamine ELISA test kit (Life Science Format) is a competitive direct ELISA (Enzyme-Linked ImmunosorbentAssay) in a microwell format that allows users to obtain exact concentrations of histamine in nanograms per milliliter
This kit is designed for in vitro quantification of histamine in various biological fluids by competitive direct enzyme-linked immunosorbent assay (CD-ELISA) This kit is intended for use in investigative research only and not for human clinical diagnostic use
This Histamine ELISA kit (Life Science Format) should not be used for determining histamine levels in scombroid fish Neogenoffers a separate kit for determination of histamine in fish and other foods
Intended User Researchers in biomedical fields
For Research Use Only
The Role of Histamine in Radiationrsquo Toxic Effects
Kit Contents
Histamine Antibody-Coated Microplate (96 well)
Histamine Standards (Ready-to-use)
Histamine Enzyme Conjugate (HRP)
K-Blue Substrate (TMB)
Wash Buffer (25x)
PBS Sample Diluent
Instruction Booklet
The Role of Histamine in Radiationrsquo Toxic Effects
Cross-reactivity
Histamine 10000
Histidine 0008
Cadaverine 0003
Tyramine lt001
Spermine lt001
Putrescine lt001
Trimethylamine lt001
The Role of Histamine in Radiationrsquo Toxic Effects
Specifications
Sensitivity70 BB0 25 ngmL30 BB0 10 ngmL
Assay Range 25 ndash 500 ngmLInter-Assay CV le10 Intra-Assay CV le10 Size 96 well microplateAssay Sample Size 50 μL Total Assay Incubation Time 15 hours Wavelength 650 nm 450 nm with acid stop Storage Conditions 4degC Antibody Monoclonal
The Role of Histamine in Radiationrsquo Toxic Effects
Recommended
For more information please contact us at
Neogen Corporation
Life Science
944 Nandino Boulevard
Lexington KY 40511 USA
800477-8201 (USACanada)
859254-1221
Fax 859255-5532
informneogencom
The Role of Histamine in Radiationrsquo Toxic Effects
Histamine release occurs when allergens bind to
mast-cell-bound IgE antibodies Reduction of IgE
overproduction may lower the likelihood of
allergens finding sufficient free IgE to trigger a
mast-cell-release of histamine
How radiation can play a role of allergens and
trigger a mast-cell-release of histamine
The Role of Histamine in Radiationrsquo Toxic Effects
Histamine H1receptor
CNS Produced in the tuberomamillary nucleus
projecting to thedorsal raphe locus coeruleus
and either to andor through thehippocampal
formation amygdala basal
ganglia thalamus superior colliculus
and cerebellum
PNS Smooth muscle and endothelium
The Role of Histamine in Radiationrsquo Toxic Effects
CNS sleep-wake cycle body
temperature nociception endocrine
homeostasisappetite mood learning
and memory
PNS Causes bronchoconstriction
bronchial smooth muscle contractionvasodilation
separation of endothelial cells (responsible
for hives) and pain and itching due to insect
stings the primary receptors involved in allergic
rhinitis symptoms and motion sickness
The Role of Histamine in Radiationrsquo Toxic Effects
Histamine H2receptor
Located on parietal cells and vascular smooth
muscle cells Primarily involved in vasodilation
Also stimulate gastric acid secretion
Histamine H3receptor Found on central nervous
system and to a lesser extent peripheral nervous
system tissue
Decreased neurotransmitter release
histamine acetylcholine norepinephrine
serotonin
The Role of Histamine in Radiationrsquo Toxic Effects
Histamine H4receptor Found primarily in
the basophils and in the bone marrow
It is also found on thymus small
intestine spleen and colon
Plays a role in mast cell chemotaxis
The Role of Histamine in Radiationrsquo Toxic Effects
Tissue mast cells which have been traditionally
studied in the context of post-radiation allergic
inflammation are not well studied and remain
controversial to play a role in activation both innate
and acquired immunity after irradiation
The Role of Histamine in Radiationrsquo Toxic Effects
A qualitative risk assessment of biogenic amines (BA) after irradiation in blood lymph and tissues was conducted using data from the scientific literature as well as original research studies conducted
Histamine and serotonin are considered as the potentially toxic substances after irradiation
Concern due to associated intensive irradiation and enzyme activity and potential for immune system toxic and autoimmune response with auto antibodies and Biogenic Amines formation
The Role of Histamine in Radiationrsquos
Toxic Effects
Scombroid fish poisoning (often called histamine poisoning) is caused by ingestion of certain species of marine fish that contain high levels of histamine and possibly other biogenic amines These fish species contain high levels of free histidine in their tissue and when such fish are subjected to temperature abuse after harvest bacteria decarboxylate histidine to produce histamine A number of bacterial species can produce histamine but Morganellamorganii and Proteus spp are particularly strong histamine producers
The Role of Histamine in Radiationrsquo Toxic Effects
Histamine playing important role in food toxicity
ldquoHistamine is an organic nitrogen compound that may trigger an allergic response in human and mammalian systems Other biogenic amines (such as cadaverine and putrescine) are thought to potentiate the toxic effect of histamine Although fatal cases are rare severity of the symptoms can vary depending on the amount of histamine and other biogenic amines ingested and the individualrsquos sensitivity to specific biogenic aminesrdquo WHO httpwwwwhointfoodsafetyhistamineen
The Role of Histamine in Radiationrsquo Toxic Effects
Scombroid fish poisoning (often called histamine
poisoning) is caused by ingestion of certain species
of marine fish that contain high levels of histamine
and possibly other biogenic amines These fish
species contain high levels of free histidine in their
tissue and when such fish are subjected to
temperature abuse after harvest bacteria
decarboxylate histidine to produce histamine A
number of bacterial species can produce histamine
but Morganella morganii and Proteus spp are
particularly strong histamine producers
httpwwwwhointfoodsafetyhistamineen
The Role of Histamine in Radiationrsquo Toxic Effects
Eating food (fish particularly) with high levels of
histamine can result in Histamine Fish Poisoning
HFP
The symptoms of HFP can be very similar to
severe allergic reactions which can happens
within minutes to hours after ingestion of toxic fish
with high levels of Histamine in tissue
Symptoms of Histamine poisoning include
A burningtingling sensation around of mouth
Rush and or swelling over the chest andor neck
The Role of Histamine in Radiationrsquo Toxic Effects
Lowering of blood pressure
Headache
Itchy skin vomiting diarrhea
Symptoms of irradiation
The prodrome (early symptoms) of Acute Radiation Syndrome typically includes nausea and vomiting headaches fatigue fever and short period of skin reddening These symptoms may occur at radiation doses as low as 35 rad (035 Gy) These symptoms are common to many illnesses (Include HFP) and may not by themselves indicate acute radiation sickness
The Role of Histamine in Radiationrsquo s Toxic Effects The role of Histamine in development of Radiationrsquo s toxic
effects remain very controversial and need to be reconsidered
1 Scientists reported that histamine and biogenic amines playing extremely active role in development patho-physiological consequences after irradiation httpwwwintechopencombookscurrent-topics-in-ionizing-radiation-researchradiation-toxins-molecular-mechanisms-of-toxicity-and-radiomimetic-properties-
Radiation Toxicity A Practical Medical Guide
edited by William Small Gayle E Woloschak
2 Scientists reported that histamine and biogenic amines donrsquot play any role in development patho-physiological consequences and clinical signs after irradiation
The Role of Histamine in Radiationrsquo Toxic Effects 3 Scientists reported that histamine and biogenic amines
playing extremely active role in development Radioprotective mechanisms after irradiation
ldquoHistamine prevents radiation-induced toxicity by increasing proliferation of damaged intestinal mucosa and suppressing apoptosis that was associated with an increase in SOD and Catalase levels This effect might be of clinical value in patients undergoing radiotherapyrdquo Int J Radiat Biol 2007 Oct83(10)653-63
Mechanisms underlying the radioprotective effect of histamine on small intestine
Medina VA1 Croci M Mohamad NA Massari N GarbarinoG Cricco GP Nuacutentildeez MA Martiacuten GA Crescenti EJ BergocRM Rivera ES
The Role of Histamine in Radiationrsquo Toxic Effects Recommended Histamine ELISA Test Kit
Neogenrsquos Histamine ELISA test kit (Life Science Format) is a competitive direct ELISA (Enzyme-Linked ImmunosorbentAssay) in a microwell format that allows users to obtain exact concentrations of histamine in nanograms per milliliter
This kit is designed for in vitro quantification of histamine in various biological fluids by competitive direct enzyme-linked immunosorbent assay (CD-ELISA) This kit is intended for use in investigative research only and not for human clinical diagnostic use
This Histamine ELISA kit (Life Science Format) should not be used for determining histamine levels in scombroid fish Neogenoffers a separate kit for determination of histamine in fish and other foods
Intended User Researchers in biomedical fields
For Research Use Only
The Role of Histamine in Radiationrsquo Toxic Effects
Kit Contents
Histamine Antibody-Coated Microplate (96 well)
Histamine Standards (Ready-to-use)
Histamine Enzyme Conjugate (HRP)
K-Blue Substrate (TMB)
Wash Buffer (25x)
PBS Sample Diluent
Instruction Booklet
The Role of Histamine in Radiationrsquo Toxic Effects
Cross-reactivity
Histamine 10000
Histidine 0008
Cadaverine 0003
Tyramine lt001
Spermine lt001
Putrescine lt001
Trimethylamine lt001
The Role of Histamine in Radiationrsquo Toxic Effects
Specifications
Sensitivity70 BB0 25 ngmL30 BB0 10 ngmL
Assay Range 25 ndash 500 ngmLInter-Assay CV le10 Intra-Assay CV le10 Size 96 well microplateAssay Sample Size 50 μL Total Assay Incubation Time 15 hours Wavelength 650 nm 450 nm with acid stop Storage Conditions 4degC Antibody Monoclonal
The Role of Histamine in Radiationrsquo Toxic Effects
Recommended
For more information please contact us at
Neogen Corporation
Life Science
944 Nandino Boulevard
Lexington KY 40511 USA
800477-8201 (USACanada)
859254-1221
Fax 859255-5532
informneogencom
The Role of Histamine in Radiationrsquo Toxic Effects
Histamine H1receptor
CNS Produced in the tuberomamillary nucleus
projecting to thedorsal raphe locus coeruleus
and either to andor through thehippocampal
formation amygdala basal
ganglia thalamus superior colliculus
and cerebellum
PNS Smooth muscle and endothelium
The Role of Histamine in Radiationrsquo Toxic Effects
CNS sleep-wake cycle body
temperature nociception endocrine
homeostasisappetite mood learning
and memory
PNS Causes bronchoconstriction
bronchial smooth muscle contractionvasodilation
separation of endothelial cells (responsible
for hives) and pain and itching due to insect
stings the primary receptors involved in allergic
rhinitis symptoms and motion sickness
The Role of Histamine in Radiationrsquo Toxic Effects
Histamine H2receptor
Located on parietal cells and vascular smooth
muscle cells Primarily involved in vasodilation
Also stimulate gastric acid secretion
Histamine H3receptor Found on central nervous
system and to a lesser extent peripheral nervous
system tissue
Decreased neurotransmitter release
histamine acetylcholine norepinephrine
serotonin
The Role of Histamine in Radiationrsquo Toxic Effects
Histamine H4receptor Found primarily in
the basophils and in the bone marrow
It is also found on thymus small
intestine spleen and colon
Plays a role in mast cell chemotaxis
The Role of Histamine in Radiationrsquo Toxic Effects
Tissue mast cells which have been traditionally
studied in the context of post-radiation allergic
inflammation are not well studied and remain
controversial to play a role in activation both innate
and acquired immunity after irradiation
The Role of Histamine in Radiationrsquo Toxic Effects
A qualitative risk assessment of biogenic amines (BA) after irradiation in blood lymph and tissues was conducted using data from the scientific literature as well as original research studies conducted
Histamine and serotonin are considered as the potentially toxic substances after irradiation
Concern due to associated intensive irradiation and enzyme activity and potential for immune system toxic and autoimmune response with auto antibodies and Biogenic Amines formation
The Role of Histamine in Radiationrsquos
Toxic Effects
Scombroid fish poisoning (often called histamine poisoning) is caused by ingestion of certain species of marine fish that contain high levels of histamine and possibly other biogenic amines These fish species contain high levels of free histidine in their tissue and when such fish are subjected to temperature abuse after harvest bacteria decarboxylate histidine to produce histamine A number of bacterial species can produce histamine but Morganellamorganii and Proteus spp are particularly strong histamine producers
The Role of Histamine in Radiationrsquo Toxic Effects
Histamine playing important role in food toxicity
ldquoHistamine is an organic nitrogen compound that may trigger an allergic response in human and mammalian systems Other biogenic amines (such as cadaverine and putrescine) are thought to potentiate the toxic effect of histamine Although fatal cases are rare severity of the symptoms can vary depending on the amount of histamine and other biogenic amines ingested and the individualrsquos sensitivity to specific biogenic aminesrdquo WHO httpwwwwhointfoodsafetyhistamineen
The Role of Histamine in Radiationrsquo Toxic Effects
Scombroid fish poisoning (often called histamine
poisoning) is caused by ingestion of certain species
of marine fish that contain high levels of histamine
and possibly other biogenic amines These fish
species contain high levels of free histidine in their
tissue and when such fish are subjected to
temperature abuse after harvest bacteria
decarboxylate histidine to produce histamine A
number of bacterial species can produce histamine
but Morganella morganii and Proteus spp are
particularly strong histamine producers
httpwwwwhointfoodsafetyhistamineen
The Role of Histamine in Radiationrsquo Toxic Effects
Eating food (fish particularly) with high levels of
histamine can result in Histamine Fish Poisoning
HFP
The symptoms of HFP can be very similar to
severe allergic reactions which can happens
within minutes to hours after ingestion of toxic fish
with high levels of Histamine in tissue
Symptoms of Histamine poisoning include
A burningtingling sensation around of mouth
Rush and or swelling over the chest andor neck
The Role of Histamine in Radiationrsquo Toxic Effects
Lowering of blood pressure
Headache
Itchy skin vomiting diarrhea
Symptoms of irradiation
The prodrome (early symptoms) of Acute Radiation Syndrome typically includes nausea and vomiting headaches fatigue fever and short period of skin reddening These symptoms may occur at radiation doses as low as 35 rad (035 Gy) These symptoms are common to many illnesses (Include HFP) and may not by themselves indicate acute radiation sickness
The Role of Histamine in Radiationrsquo s Toxic Effects The role of Histamine in development of Radiationrsquo s toxic
effects remain very controversial and need to be reconsidered
1 Scientists reported that histamine and biogenic amines playing extremely active role in development patho-physiological consequences after irradiation httpwwwintechopencombookscurrent-topics-in-ionizing-radiation-researchradiation-toxins-molecular-mechanisms-of-toxicity-and-radiomimetic-properties-
Radiation Toxicity A Practical Medical Guide
edited by William Small Gayle E Woloschak
2 Scientists reported that histamine and biogenic amines donrsquot play any role in development patho-physiological consequences and clinical signs after irradiation
The Role of Histamine in Radiationrsquo Toxic Effects 3 Scientists reported that histamine and biogenic amines
playing extremely active role in development Radioprotective mechanisms after irradiation
ldquoHistamine prevents radiation-induced toxicity by increasing proliferation of damaged intestinal mucosa and suppressing apoptosis that was associated with an increase in SOD and Catalase levels This effect might be of clinical value in patients undergoing radiotherapyrdquo Int J Radiat Biol 2007 Oct83(10)653-63
Mechanisms underlying the radioprotective effect of histamine on small intestine
Medina VA1 Croci M Mohamad NA Massari N GarbarinoG Cricco GP Nuacutentildeez MA Martiacuten GA Crescenti EJ BergocRM Rivera ES
The Role of Histamine in Radiationrsquo Toxic Effects Recommended Histamine ELISA Test Kit
Neogenrsquos Histamine ELISA test kit (Life Science Format) is a competitive direct ELISA (Enzyme-Linked ImmunosorbentAssay) in a microwell format that allows users to obtain exact concentrations of histamine in nanograms per milliliter
This kit is designed for in vitro quantification of histamine in various biological fluids by competitive direct enzyme-linked immunosorbent assay (CD-ELISA) This kit is intended for use in investigative research only and not for human clinical diagnostic use
This Histamine ELISA kit (Life Science Format) should not be used for determining histamine levels in scombroid fish Neogenoffers a separate kit for determination of histamine in fish and other foods
Intended User Researchers in biomedical fields
For Research Use Only
The Role of Histamine in Radiationrsquo Toxic Effects
Kit Contents
Histamine Antibody-Coated Microplate (96 well)
Histamine Standards (Ready-to-use)
Histamine Enzyme Conjugate (HRP)
K-Blue Substrate (TMB)
Wash Buffer (25x)
PBS Sample Diluent
Instruction Booklet
The Role of Histamine in Radiationrsquo Toxic Effects
Cross-reactivity
Histamine 10000
Histidine 0008
Cadaverine 0003
Tyramine lt001
Spermine lt001
Putrescine lt001
Trimethylamine lt001
The Role of Histamine in Radiationrsquo Toxic Effects
Specifications
Sensitivity70 BB0 25 ngmL30 BB0 10 ngmL
Assay Range 25 ndash 500 ngmLInter-Assay CV le10 Intra-Assay CV le10 Size 96 well microplateAssay Sample Size 50 μL Total Assay Incubation Time 15 hours Wavelength 650 nm 450 nm with acid stop Storage Conditions 4degC Antibody Monoclonal
The Role of Histamine in Radiationrsquo Toxic Effects
Recommended
For more information please contact us at
Neogen Corporation
Life Science
944 Nandino Boulevard
Lexington KY 40511 USA
800477-8201 (USACanada)
859254-1221
Fax 859255-5532
informneogencom
The Role of Histamine in Radiationrsquo Toxic Effects
CNS sleep-wake cycle body
temperature nociception endocrine
homeostasisappetite mood learning
and memory
PNS Causes bronchoconstriction
bronchial smooth muscle contractionvasodilation
separation of endothelial cells (responsible
for hives) and pain and itching due to insect
stings the primary receptors involved in allergic
rhinitis symptoms and motion sickness
The Role of Histamine in Radiationrsquo Toxic Effects
Histamine H2receptor
Located on parietal cells and vascular smooth
muscle cells Primarily involved in vasodilation
Also stimulate gastric acid secretion
Histamine H3receptor Found on central nervous
system and to a lesser extent peripheral nervous
system tissue
Decreased neurotransmitter release
histamine acetylcholine norepinephrine
serotonin
The Role of Histamine in Radiationrsquo Toxic Effects
Histamine H4receptor Found primarily in
the basophils and in the bone marrow
It is also found on thymus small
intestine spleen and colon
Plays a role in mast cell chemotaxis
The Role of Histamine in Radiationrsquo Toxic Effects
Tissue mast cells which have been traditionally
studied in the context of post-radiation allergic
inflammation are not well studied and remain
controversial to play a role in activation both innate
and acquired immunity after irradiation
The Role of Histamine in Radiationrsquo Toxic Effects
A qualitative risk assessment of biogenic amines (BA) after irradiation in blood lymph and tissues was conducted using data from the scientific literature as well as original research studies conducted
Histamine and serotonin are considered as the potentially toxic substances after irradiation
Concern due to associated intensive irradiation and enzyme activity and potential for immune system toxic and autoimmune response with auto antibodies and Biogenic Amines formation
The Role of Histamine in Radiationrsquos
Toxic Effects
Scombroid fish poisoning (often called histamine poisoning) is caused by ingestion of certain species of marine fish that contain high levels of histamine and possibly other biogenic amines These fish species contain high levels of free histidine in their tissue and when such fish are subjected to temperature abuse after harvest bacteria decarboxylate histidine to produce histamine A number of bacterial species can produce histamine but Morganellamorganii and Proteus spp are particularly strong histamine producers
The Role of Histamine in Radiationrsquo Toxic Effects
Histamine playing important role in food toxicity
ldquoHistamine is an organic nitrogen compound that may trigger an allergic response in human and mammalian systems Other biogenic amines (such as cadaverine and putrescine) are thought to potentiate the toxic effect of histamine Although fatal cases are rare severity of the symptoms can vary depending on the amount of histamine and other biogenic amines ingested and the individualrsquos sensitivity to specific biogenic aminesrdquo WHO httpwwwwhointfoodsafetyhistamineen
The Role of Histamine in Radiationrsquo Toxic Effects
Scombroid fish poisoning (often called histamine
poisoning) is caused by ingestion of certain species
of marine fish that contain high levels of histamine
and possibly other biogenic amines These fish
species contain high levels of free histidine in their
tissue and when such fish are subjected to
temperature abuse after harvest bacteria
decarboxylate histidine to produce histamine A
number of bacterial species can produce histamine
but Morganella morganii and Proteus spp are
particularly strong histamine producers
httpwwwwhointfoodsafetyhistamineen
The Role of Histamine in Radiationrsquo Toxic Effects
Eating food (fish particularly) with high levels of
histamine can result in Histamine Fish Poisoning
HFP
The symptoms of HFP can be very similar to
severe allergic reactions which can happens
within minutes to hours after ingestion of toxic fish
with high levels of Histamine in tissue
Symptoms of Histamine poisoning include
A burningtingling sensation around of mouth
Rush and or swelling over the chest andor neck
The Role of Histamine in Radiationrsquo Toxic Effects
Lowering of blood pressure
Headache
Itchy skin vomiting diarrhea
Symptoms of irradiation
The prodrome (early symptoms) of Acute Radiation Syndrome typically includes nausea and vomiting headaches fatigue fever and short period of skin reddening These symptoms may occur at radiation doses as low as 35 rad (035 Gy) These symptoms are common to many illnesses (Include HFP) and may not by themselves indicate acute radiation sickness
The Role of Histamine in Radiationrsquo s Toxic Effects The role of Histamine in development of Radiationrsquo s toxic
effects remain very controversial and need to be reconsidered
1 Scientists reported that histamine and biogenic amines playing extremely active role in development patho-physiological consequences after irradiation httpwwwintechopencombookscurrent-topics-in-ionizing-radiation-researchradiation-toxins-molecular-mechanisms-of-toxicity-and-radiomimetic-properties-
Radiation Toxicity A Practical Medical Guide
edited by William Small Gayle E Woloschak
2 Scientists reported that histamine and biogenic amines donrsquot play any role in development patho-physiological consequences and clinical signs after irradiation
The Role of Histamine in Radiationrsquo Toxic Effects 3 Scientists reported that histamine and biogenic amines
playing extremely active role in development Radioprotective mechanisms after irradiation
ldquoHistamine prevents radiation-induced toxicity by increasing proliferation of damaged intestinal mucosa and suppressing apoptosis that was associated with an increase in SOD and Catalase levels This effect might be of clinical value in patients undergoing radiotherapyrdquo Int J Radiat Biol 2007 Oct83(10)653-63
Mechanisms underlying the radioprotective effect of histamine on small intestine
Medina VA1 Croci M Mohamad NA Massari N GarbarinoG Cricco GP Nuacutentildeez MA Martiacuten GA Crescenti EJ BergocRM Rivera ES
The Role of Histamine in Radiationrsquo Toxic Effects Recommended Histamine ELISA Test Kit
Neogenrsquos Histamine ELISA test kit (Life Science Format) is a competitive direct ELISA (Enzyme-Linked ImmunosorbentAssay) in a microwell format that allows users to obtain exact concentrations of histamine in nanograms per milliliter
This kit is designed for in vitro quantification of histamine in various biological fluids by competitive direct enzyme-linked immunosorbent assay (CD-ELISA) This kit is intended for use in investigative research only and not for human clinical diagnostic use
This Histamine ELISA kit (Life Science Format) should not be used for determining histamine levels in scombroid fish Neogenoffers a separate kit for determination of histamine in fish and other foods
Intended User Researchers in biomedical fields
For Research Use Only
The Role of Histamine in Radiationrsquo Toxic Effects
Kit Contents
Histamine Antibody-Coated Microplate (96 well)
Histamine Standards (Ready-to-use)
Histamine Enzyme Conjugate (HRP)
K-Blue Substrate (TMB)
Wash Buffer (25x)
PBS Sample Diluent
Instruction Booklet
The Role of Histamine in Radiationrsquo Toxic Effects
Cross-reactivity
Histamine 10000
Histidine 0008
Cadaverine 0003
Tyramine lt001
Spermine lt001
Putrescine lt001
Trimethylamine lt001
The Role of Histamine in Radiationrsquo Toxic Effects
Specifications
Sensitivity70 BB0 25 ngmL30 BB0 10 ngmL
Assay Range 25 ndash 500 ngmLInter-Assay CV le10 Intra-Assay CV le10 Size 96 well microplateAssay Sample Size 50 μL Total Assay Incubation Time 15 hours Wavelength 650 nm 450 nm with acid stop Storage Conditions 4degC Antibody Monoclonal
The Role of Histamine in Radiationrsquo Toxic Effects
Recommended
For more information please contact us at
Neogen Corporation
Life Science
944 Nandino Boulevard
Lexington KY 40511 USA
800477-8201 (USACanada)
859254-1221
Fax 859255-5532
informneogencom
The Role of Histamine in Radiationrsquo Toxic Effects
Histamine H2receptor
Located on parietal cells and vascular smooth
muscle cells Primarily involved in vasodilation
Also stimulate gastric acid secretion
Histamine H3receptor Found on central nervous
system and to a lesser extent peripheral nervous
system tissue
Decreased neurotransmitter release
histamine acetylcholine norepinephrine
serotonin
The Role of Histamine in Radiationrsquo Toxic Effects
Histamine H4receptor Found primarily in
the basophils and in the bone marrow
It is also found on thymus small
intestine spleen and colon
Plays a role in mast cell chemotaxis
The Role of Histamine in Radiationrsquo Toxic Effects
Tissue mast cells which have been traditionally
studied in the context of post-radiation allergic
inflammation are not well studied and remain
controversial to play a role in activation both innate
and acquired immunity after irradiation
The Role of Histamine in Radiationrsquo Toxic Effects
A qualitative risk assessment of biogenic amines (BA) after irradiation in blood lymph and tissues was conducted using data from the scientific literature as well as original research studies conducted
Histamine and serotonin are considered as the potentially toxic substances after irradiation
Concern due to associated intensive irradiation and enzyme activity and potential for immune system toxic and autoimmune response with auto antibodies and Biogenic Amines formation
The Role of Histamine in Radiationrsquos
Toxic Effects
Scombroid fish poisoning (often called histamine poisoning) is caused by ingestion of certain species of marine fish that contain high levels of histamine and possibly other biogenic amines These fish species contain high levels of free histidine in their tissue and when such fish are subjected to temperature abuse after harvest bacteria decarboxylate histidine to produce histamine A number of bacterial species can produce histamine but Morganellamorganii and Proteus spp are particularly strong histamine producers
The Role of Histamine in Radiationrsquo Toxic Effects
Histamine playing important role in food toxicity
ldquoHistamine is an organic nitrogen compound that may trigger an allergic response in human and mammalian systems Other biogenic amines (such as cadaverine and putrescine) are thought to potentiate the toxic effect of histamine Although fatal cases are rare severity of the symptoms can vary depending on the amount of histamine and other biogenic amines ingested and the individualrsquos sensitivity to specific biogenic aminesrdquo WHO httpwwwwhointfoodsafetyhistamineen
The Role of Histamine in Radiationrsquo Toxic Effects
Scombroid fish poisoning (often called histamine
poisoning) is caused by ingestion of certain species
of marine fish that contain high levels of histamine
and possibly other biogenic amines These fish
species contain high levels of free histidine in their
tissue and when such fish are subjected to
temperature abuse after harvest bacteria
decarboxylate histidine to produce histamine A
number of bacterial species can produce histamine
but Morganella morganii and Proteus spp are
particularly strong histamine producers
httpwwwwhointfoodsafetyhistamineen
The Role of Histamine in Radiationrsquo Toxic Effects
Eating food (fish particularly) with high levels of
histamine can result in Histamine Fish Poisoning
HFP
The symptoms of HFP can be very similar to
severe allergic reactions which can happens
within minutes to hours after ingestion of toxic fish
with high levels of Histamine in tissue
Symptoms of Histamine poisoning include
A burningtingling sensation around of mouth
Rush and or swelling over the chest andor neck
The Role of Histamine in Radiationrsquo Toxic Effects
Lowering of blood pressure
Headache
Itchy skin vomiting diarrhea
Symptoms of irradiation
The prodrome (early symptoms) of Acute Radiation Syndrome typically includes nausea and vomiting headaches fatigue fever and short period of skin reddening These symptoms may occur at radiation doses as low as 35 rad (035 Gy) These symptoms are common to many illnesses (Include HFP) and may not by themselves indicate acute radiation sickness
The Role of Histamine in Radiationrsquo s Toxic Effects The role of Histamine in development of Radiationrsquo s toxic
effects remain very controversial and need to be reconsidered
1 Scientists reported that histamine and biogenic amines playing extremely active role in development patho-physiological consequences after irradiation httpwwwintechopencombookscurrent-topics-in-ionizing-radiation-researchradiation-toxins-molecular-mechanisms-of-toxicity-and-radiomimetic-properties-
Radiation Toxicity A Practical Medical Guide
edited by William Small Gayle E Woloschak
2 Scientists reported that histamine and biogenic amines donrsquot play any role in development patho-physiological consequences and clinical signs after irradiation
The Role of Histamine in Radiationrsquo Toxic Effects 3 Scientists reported that histamine and biogenic amines
playing extremely active role in development Radioprotective mechanisms after irradiation
ldquoHistamine prevents radiation-induced toxicity by increasing proliferation of damaged intestinal mucosa and suppressing apoptosis that was associated with an increase in SOD and Catalase levels This effect might be of clinical value in patients undergoing radiotherapyrdquo Int J Radiat Biol 2007 Oct83(10)653-63
Mechanisms underlying the radioprotective effect of histamine on small intestine
Medina VA1 Croci M Mohamad NA Massari N GarbarinoG Cricco GP Nuacutentildeez MA Martiacuten GA Crescenti EJ BergocRM Rivera ES
The Role of Histamine in Radiationrsquo Toxic Effects Recommended Histamine ELISA Test Kit
Neogenrsquos Histamine ELISA test kit (Life Science Format) is a competitive direct ELISA (Enzyme-Linked ImmunosorbentAssay) in a microwell format that allows users to obtain exact concentrations of histamine in nanograms per milliliter
This kit is designed for in vitro quantification of histamine in various biological fluids by competitive direct enzyme-linked immunosorbent assay (CD-ELISA) This kit is intended for use in investigative research only and not for human clinical diagnostic use
This Histamine ELISA kit (Life Science Format) should not be used for determining histamine levels in scombroid fish Neogenoffers a separate kit for determination of histamine in fish and other foods
Intended User Researchers in biomedical fields
For Research Use Only
The Role of Histamine in Radiationrsquo Toxic Effects
Kit Contents
Histamine Antibody-Coated Microplate (96 well)
Histamine Standards (Ready-to-use)
Histamine Enzyme Conjugate (HRP)
K-Blue Substrate (TMB)
Wash Buffer (25x)
PBS Sample Diluent
Instruction Booklet
The Role of Histamine in Radiationrsquo Toxic Effects
Cross-reactivity
Histamine 10000
Histidine 0008
Cadaverine 0003
Tyramine lt001
Spermine lt001
Putrescine lt001
Trimethylamine lt001
The Role of Histamine in Radiationrsquo Toxic Effects
Specifications
Sensitivity70 BB0 25 ngmL30 BB0 10 ngmL
Assay Range 25 ndash 500 ngmLInter-Assay CV le10 Intra-Assay CV le10 Size 96 well microplateAssay Sample Size 50 μL Total Assay Incubation Time 15 hours Wavelength 650 nm 450 nm with acid stop Storage Conditions 4degC Antibody Monoclonal
The Role of Histamine in Radiationrsquo Toxic Effects
Recommended
For more information please contact us at
Neogen Corporation
Life Science
944 Nandino Boulevard
Lexington KY 40511 USA
800477-8201 (USACanada)
859254-1221
Fax 859255-5532
informneogencom
The Role of Histamine in Radiationrsquo Toxic Effects
Histamine H4receptor Found primarily in
the basophils and in the bone marrow
It is also found on thymus small
intestine spleen and colon
Plays a role in mast cell chemotaxis
The Role of Histamine in Radiationrsquo Toxic Effects
Tissue mast cells which have been traditionally
studied in the context of post-radiation allergic
inflammation are not well studied and remain
controversial to play a role in activation both innate
and acquired immunity after irradiation
The Role of Histamine in Radiationrsquo Toxic Effects
A qualitative risk assessment of biogenic amines (BA) after irradiation in blood lymph and tissues was conducted using data from the scientific literature as well as original research studies conducted
Histamine and serotonin are considered as the potentially toxic substances after irradiation
Concern due to associated intensive irradiation and enzyme activity and potential for immune system toxic and autoimmune response with auto antibodies and Biogenic Amines formation
The Role of Histamine in Radiationrsquos
Toxic Effects
Scombroid fish poisoning (often called histamine poisoning) is caused by ingestion of certain species of marine fish that contain high levels of histamine and possibly other biogenic amines These fish species contain high levels of free histidine in their tissue and when such fish are subjected to temperature abuse after harvest bacteria decarboxylate histidine to produce histamine A number of bacterial species can produce histamine but Morganellamorganii and Proteus spp are particularly strong histamine producers
The Role of Histamine in Radiationrsquo Toxic Effects
Histamine playing important role in food toxicity
ldquoHistamine is an organic nitrogen compound that may trigger an allergic response in human and mammalian systems Other biogenic amines (such as cadaverine and putrescine) are thought to potentiate the toxic effect of histamine Although fatal cases are rare severity of the symptoms can vary depending on the amount of histamine and other biogenic amines ingested and the individualrsquos sensitivity to specific biogenic aminesrdquo WHO httpwwwwhointfoodsafetyhistamineen
The Role of Histamine in Radiationrsquo Toxic Effects
Scombroid fish poisoning (often called histamine
poisoning) is caused by ingestion of certain species
of marine fish that contain high levels of histamine
and possibly other biogenic amines These fish
species contain high levels of free histidine in their
tissue and when such fish are subjected to
temperature abuse after harvest bacteria
decarboxylate histidine to produce histamine A
number of bacterial species can produce histamine
but Morganella morganii and Proteus spp are
particularly strong histamine producers
httpwwwwhointfoodsafetyhistamineen
The Role of Histamine in Radiationrsquo Toxic Effects
Eating food (fish particularly) with high levels of
histamine can result in Histamine Fish Poisoning
HFP
The symptoms of HFP can be very similar to
severe allergic reactions which can happens
within minutes to hours after ingestion of toxic fish
with high levels of Histamine in tissue
Symptoms of Histamine poisoning include
A burningtingling sensation around of mouth
Rush and or swelling over the chest andor neck
The Role of Histamine in Radiationrsquo Toxic Effects
Lowering of blood pressure
Headache
Itchy skin vomiting diarrhea
Symptoms of irradiation
The prodrome (early symptoms) of Acute Radiation Syndrome typically includes nausea and vomiting headaches fatigue fever and short period of skin reddening These symptoms may occur at radiation doses as low as 35 rad (035 Gy) These symptoms are common to many illnesses (Include HFP) and may not by themselves indicate acute radiation sickness
The Role of Histamine in Radiationrsquo s Toxic Effects The role of Histamine in development of Radiationrsquo s toxic
effects remain very controversial and need to be reconsidered
1 Scientists reported that histamine and biogenic amines playing extremely active role in development patho-physiological consequences after irradiation httpwwwintechopencombookscurrent-topics-in-ionizing-radiation-researchradiation-toxins-molecular-mechanisms-of-toxicity-and-radiomimetic-properties-
Radiation Toxicity A Practical Medical Guide
edited by William Small Gayle E Woloschak
2 Scientists reported that histamine and biogenic amines donrsquot play any role in development patho-physiological consequences and clinical signs after irradiation
The Role of Histamine in Radiationrsquo Toxic Effects 3 Scientists reported that histamine and biogenic amines
playing extremely active role in development Radioprotective mechanisms after irradiation
ldquoHistamine prevents radiation-induced toxicity by increasing proliferation of damaged intestinal mucosa and suppressing apoptosis that was associated with an increase in SOD and Catalase levels This effect might be of clinical value in patients undergoing radiotherapyrdquo Int J Radiat Biol 2007 Oct83(10)653-63
Mechanisms underlying the radioprotective effect of histamine on small intestine
Medina VA1 Croci M Mohamad NA Massari N GarbarinoG Cricco GP Nuacutentildeez MA Martiacuten GA Crescenti EJ BergocRM Rivera ES
The Role of Histamine in Radiationrsquo Toxic Effects Recommended Histamine ELISA Test Kit
Neogenrsquos Histamine ELISA test kit (Life Science Format) is a competitive direct ELISA (Enzyme-Linked ImmunosorbentAssay) in a microwell format that allows users to obtain exact concentrations of histamine in nanograms per milliliter
This kit is designed for in vitro quantification of histamine in various biological fluids by competitive direct enzyme-linked immunosorbent assay (CD-ELISA) This kit is intended for use in investigative research only and not for human clinical diagnostic use
This Histamine ELISA kit (Life Science Format) should not be used for determining histamine levels in scombroid fish Neogenoffers a separate kit for determination of histamine in fish and other foods
Intended User Researchers in biomedical fields
For Research Use Only
The Role of Histamine in Radiationrsquo Toxic Effects
Kit Contents
Histamine Antibody-Coated Microplate (96 well)
Histamine Standards (Ready-to-use)
Histamine Enzyme Conjugate (HRP)
K-Blue Substrate (TMB)
Wash Buffer (25x)
PBS Sample Diluent
Instruction Booklet
The Role of Histamine in Radiationrsquo Toxic Effects
Cross-reactivity
Histamine 10000
Histidine 0008
Cadaverine 0003
Tyramine lt001
Spermine lt001
Putrescine lt001
Trimethylamine lt001
The Role of Histamine in Radiationrsquo Toxic Effects
Specifications
Sensitivity70 BB0 25 ngmL30 BB0 10 ngmL
Assay Range 25 ndash 500 ngmLInter-Assay CV le10 Intra-Assay CV le10 Size 96 well microplateAssay Sample Size 50 μL Total Assay Incubation Time 15 hours Wavelength 650 nm 450 nm with acid stop Storage Conditions 4degC Antibody Monoclonal
The Role of Histamine in Radiationrsquo Toxic Effects
Recommended
For more information please contact us at
Neogen Corporation
Life Science
944 Nandino Boulevard
Lexington KY 40511 USA
800477-8201 (USACanada)
859254-1221
Fax 859255-5532
informneogencom
The Role of Histamine in Radiationrsquo Toxic Effects
Tissue mast cells which have been traditionally
studied in the context of post-radiation allergic
inflammation are not well studied and remain
controversial to play a role in activation both innate
and acquired immunity after irradiation
The Role of Histamine in Radiationrsquo Toxic Effects
A qualitative risk assessment of biogenic amines (BA) after irradiation in blood lymph and tissues was conducted using data from the scientific literature as well as original research studies conducted
Histamine and serotonin are considered as the potentially toxic substances after irradiation
Concern due to associated intensive irradiation and enzyme activity and potential for immune system toxic and autoimmune response with auto antibodies and Biogenic Amines formation
The Role of Histamine in Radiationrsquos
Toxic Effects
Scombroid fish poisoning (often called histamine poisoning) is caused by ingestion of certain species of marine fish that contain high levels of histamine and possibly other biogenic amines These fish species contain high levels of free histidine in their tissue and when such fish are subjected to temperature abuse after harvest bacteria decarboxylate histidine to produce histamine A number of bacterial species can produce histamine but Morganellamorganii and Proteus spp are particularly strong histamine producers
The Role of Histamine in Radiationrsquo Toxic Effects
Histamine playing important role in food toxicity
ldquoHistamine is an organic nitrogen compound that may trigger an allergic response in human and mammalian systems Other biogenic amines (such as cadaverine and putrescine) are thought to potentiate the toxic effect of histamine Although fatal cases are rare severity of the symptoms can vary depending on the amount of histamine and other biogenic amines ingested and the individualrsquos sensitivity to specific biogenic aminesrdquo WHO httpwwwwhointfoodsafetyhistamineen
The Role of Histamine in Radiationrsquo Toxic Effects
Scombroid fish poisoning (often called histamine
poisoning) is caused by ingestion of certain species
of marine fish that contain high levels of histamine
and possibly other biogenic amines These fish
species contain high levels of free histidine in their
tissue and when such fish are subjected to
temperature abuse after harvest bacteria
decarboxylate histidine to produce histamine A
number of bacterial species can produce histamine
but Morganella morganii and Proteus spp are
particularly strong histamine producers
httpwwwwhointfoodsafetyhistamineen
The Role of Histamine in Radiationrsquo Toxic Effects
Eating food (fish particularly) with high levels of
histamine can result in Histamine Fish Poisoning
HFP
The symptoms of HFP can be very similar to
severe allergic reactions which can happens
within minutes to hours after ingestion of toxic fish
with high levels of Histamine in tissue
Symptoms of Histamine poisoning include
A burningtingling sensation around of mouth
Rush and or swelling over the chest andor neck
The Role of Histamine in Radiationrsquo Toxic Effects
Lowering of blood pressure
Headache
Itchy skin vomiting diarrhea
Symptoms of irradiation
The prodrome (early symptoms) of Acute Radiation Syndrome typically includes nausea and vomiting headaches fatigue fever and short period of skin reddening These symptoms may occur at radiation doses as low as 35 rad (035 Gy) These symptoms are common to many illnesses (Include HFP) and may not by themselves indicate acute radiation sickness
The Role of Histamine in Radiationrsquo s Toxic Effects The role of Histamine in development of Radiationrsquo s toxic
effects remain very controversial and need to be reconsidered
1 Scientists reported that histamine and biogenic amines playing extremely active role in development patho-physiological consequences after irradiation httpwwwintechopencombookscurrent-topics-in-ionizing-radiation-researchradiation-toxins-molecular-mechanisms-of-toxicity-and-radiomimetic-properties-
Radiation Toxicity A Practical Medical Guide
edited by William Small Gayle E Woloschak
2 Scientists reported that histamine and biogenic amines donrsquot play any role in development patho-physiological consequences and clinical signs after irradiation
The Role of Histamine in Radiationrsquo Toxic Effects 3 Scientists reported that histamine and biogenic amines
playing extremely active role in development Radioprotective mechanisms after irradiation
ldquoHistamine prevents radiation-induced toxicity by increasing proliferation of damaged intestinal mucosa and suppressing apoptosis that was associated with an increase in SOD and Catalase levels This effect might be of clinical value in patients undergoing radiotherapyrdquo Int J Radiat Biol 2007 Oct83(10)653-63
Mechanisms underlying the radioprotective effect of histamine on small intestine
Medina VA1 Croci M Mohamad NA Massari N GarbarinoG Cricco GP Nuacutentildeez MA Martiacuten GA Crescenti EJ BergocRM Rivera ES
The Role of Histamine in Radiationrsquo Toxic Effects Recommended Histamine ELISA Test Kit
Neogenrsquos Histamine ELISA test kit (Life Science Format) is a competitive direct ELISA (Enzyme-Linked ImmunosorbentAssay) in a microwell format that allows users to obtain exact concentrations of histamine in nanograms per milliliter
This kit is designed for in vitro quantification of histamine in various biological fluids by competitive direct enzyme-linked immunosorbent assay (CD-ELISA) This kit is intended for use in investigative research only and not for human clinical diagnostic use
This Histamine ELISA kit (Life Science Format) should not be used for determining histamine levels in scombroid fish Neogenoffers a separate kit for determination of histamine in fish and other foods
Intended User Researchers in biomedical fields
For Research Use Only
The Role of Histamine in Radiationrsquo Toxic Effects
Kit Contents
Histamine Antibody-Coated Microplate (96 well)
Histamine Standards (Ready-to-use)
Histamine Enzyme Conjugate (HRP)
K-Blue Substrate (TMB)
Wash Buffer (25x)
PBS Sample Diluent
Instruction Booklet
The Role of Histamine in Radiationrsquo Toxic Effects
Cross-reactivity
Histamine 10000
Histidine 0008
Cadaverine 0003
Tyramine lt001
Spermine lt001
Putrescine lt001
Trimethylamine lt001
The Role of Histamine in Radiationrsquo Toxic Effects
Specifications
Sensitivity70 BB0 25 ngmL30 BB0 10 ngmL
Assay Range 25 ndash 500 ngmLInter-Assay CV le10 Intra-Assay CV le10 Size 96 well microplateAssay Sample Size 50 μL Total Assay Incubation Time 15 hours Wavelength 650 nm 450 nm with acid stop Storage Conditions 4degC Antibody Monoclonal
The Role of Histamine in Radiationrsquo Toxic Effects
Recommended
For more information please contact us at
Neogen Corporation
Life Science
944 Nandino Boulevard
Lexington KY 40511 USA
800477-8201 (USACanada)
859254-1221
Fax 859255-5532
informneogencom
The Role of Histamine in Radiationrsquo Toxic Effects
A qualitative risk assessment of biogenic amines (BA) after irradiation in blood lymph and tissues was conducted using data from the scientific literature as well as original research studies conducted
Histamine and serotonin are considered as the potentially toxic substances after irradiation
Concern due to associated intensive irradiation and enzyme activity and potential for immune system toxic and autoimmune response with auto antibodies and Biogenic Amines formation
The Role of Histamine in Radiationrsquos
Toxic Effects
Scombroid fish poisoning (often called histamine poisoning) is caused by ingestion of certain species of marine fish that contain high levels of histamine and possibly other biogenic amines These fish species contain high levels of free histidine in their tissue and when such fish are subjected to temperature abuse after harvest bacteria decarboxylate histidine to produce histamine A number of bacterial species can produce histamine but Morganellamorganii and Proteus spp are particularly strong histamine producers
The Role of Histamine in Radiationrsquo Toxic Effects
Histamine playing important role in food toxicity
ldquoHistamine is an organic nitrogen compound that may trigger an allergic response in human and mammalian systems Other biogenic amines (such as cadaverine and putrescine) are thought to potentiate the toxic effect of histamine Although fatal cases are rare severity of the symptoms can vary depending on the amount of histamine and other biogenic amines ingested and the individualrsquos sensitivity to specific biogenic aminesrdquo WHO httpwwwwhointfoodsafetyhistamineen
The Role of Histamine in Radiationrsquo Toxic Effects
Scombroid fish poisoning (often called histamine
poisoning) is caused by ingestion of certain species
of marine fish that contain high levels of histamine
and possibly other biogenic amines These fish
species contain high levels of free histidine in their
tissue and when such fish are subjected to
temperature abuse after harvest bacteria
decarboxylate histidine to produce histamine A
number of bacterial species can produce histamine
but Morganella morganii and Proteus spp are
particularly strong histamine producers
httpwwwwhointfoodsafetyhistamineen
The Role of Histamine in Radiationrsquo Toxic Effects
Eating food (fish particularly) with high levels of
histamine can result in Histamine Fish Poisoning
HFP
The symptoms of HFP can be very similar to
severe allergic reactions which can happens
within minutes to hours after ingestion of toxic fish
with high levels of Histamine in tissue
Symptoms of Histamine poisoning include
A burningtingling sensation around of mouth
Rush and or swelling over the chest andor neck
The Role of Histamine in Radiationrsquo Toxic Effects
Lowering of blood pressure
Headache
Itchy skin vomiting diarrhea
Symptoms of irradiation
The prodrome (early symptoms) of Acute Radiation Syndrome typically includes nausea and vomiting headaches fatigue fever and short period of skin reddening These symptoms may occur at radiation doses as low as 35 rad (035 Gy) These symptoms are common to many illnesses (Include HFP) and may not by themselves indicate acute radiation sickness
The Role of Histamine in Radiationrsquo s Toxic Effects The role of Histamine in development of Radiationrsquo s toxic
effects remain very controversial and need to be reconsidered
1 Scientists reported that histamine and biogenic amines playing extremely active role in development patho-physiological consequences after irradiation httpwwwintechopencombookscurrent-topics-in-ionizing-radiation-researchradiation-toxins-molecular-mechanisms-of-toxicity-and-radiomimetic-properties-
Radiation Toxicity A Practical Medical Guide
edited by William Small Gayle E Woloschak
2 Scientists reported that histamine and biogenic amines donrsquot play any role in development patho-physiological consequences and clinical signs after irradiation
The Role of Histamine in Radiationrsquo Toxic Effects 3 Scientists reported that histamine and biogenic amines
playing extremely active role in development Radioprotective mechanisms after irradiation
ldquoHistamine prevents radiation-induced toxicity by increasing proliferation of damaged intestinal mucosa and suppressing apoptosis that was associated with an increase in SOD and Catalase levels This effect might be of clinical value in patients undergoing radiotherapyrdquo Int J Radiat Biol 2007 Oct83(10)653-63
Mechanisms underlying the radioprotective effect of histamine on small intestine
Medina VA1 Croci M Mohamad NA Massari N GarbarinoG Cricco GP Nuacutentildeez MA Martiacuten GA Crescenti EJ BergocRM Rivera ES
The Role of Histamine in Radiationrsquo Toxic Effects Recommended Histamine ELISA Test Kit
Neogenrsquos Histamine ELISA test kit (Life Science Format) is a competitive direct ELISA (Enzyme-Linked ImmunosorbentAssay) in a microwell format that allows users to obtain exact concentrations of histamine in nanograms per milliliter
This kit is designed for in vitro quantification of histamine in various biological fluids by competitive direct enzyme-linked immunosorbent assay (CD-ELISA) This kit is intended for use in investigative research only and not for human clinical diagnostic use
This Histamine ELISA kit (Life Science Format) should not be used for determining histamine levels in scombroid fish Neogenoffers a separate kit for determination of histamine in fish and other foods
Intended User Researchers in biomedical fields
For Research Use Only
The Role of Histamine in Radiationrsquo Toxic Effects
Kit Contents
Histamine Antibody-Coated Microplate (96 well)
Histamine Standards (Ready-to-use)
Histamine Enzyme Conjugate (HRP)
K-Blue Substrate (TMB)
Wash Buffer (25x)
PBS Sample Diluent
Instruction Booklet
The Role of Histamine in Radiationrsquo Toxic Effects
Cross-reactivity
Histamine 10000
Histidine 0008
Cadaverine 0003
Tyramine lt001
Spermine lt001
Putrescine lt001
Trimethylamine lt001
The Role of Histamine in Radiationrsquo Toxic Effects
Specifications
Sensitivity70 BB0 25 ngmL30 BB0 10 ngmL
Assay Range 25 ndash 500 ngmLInter-Assay CV le10 Intra-Assay CV le10 Size 96 well microplateAssay Sample Size 50 μL Total Assay Incubation Time 15 hours Wavelength 650 nm 450 nm with acid stop Storage Conditions 4degC Antibody Monoclonal
The Role of Histamine in Radiationrsquo Toxic Effects
Recommended
For more information please contact us at
Neogen Corporation
Life Science
944 Nandino Boulevard
Lexington KY 40511 USA
800477-8201 (USACanada)
859254-1221
Fax 859255-5532
informneogencom
The Role of Histamine in Radiationrsquos
Toxic Effects
Scombroid fish poisoning (often called histamine poisoning) is caused by ingestion of certain species of marine fish that contain high levels of histamine and possibly other biogenic amines These fish species contain high levels of free histidine in their tissue and when such fish are subjected to temperature abuse after harvest bacteria decarboxylate histidine to produce histamine A number of bacterial species can produce histamine but Morganellamorganii and Proteus spp are particularly strong histamine producers
The Role of Histamine in Radiationrsquo Toxic Effects
Histamine playing important role in food toxicity
ldquoHistamine is an organic nitrogen compound that may trigger an allergic response in human and mammalian systems Other biogenic amines (such as cadaverine and putrescine) are thought to potentiate the toxic effect of histamine Although fatal cases are rare severity of the symptoms can vary depending on the amount of histamine and other biogenic amines ingested and the individualrsquos sensitivity to specific biogenic aminesrdquo WHO httpwwwwhointfoodsafetyhistamineen
The Role of Histamine in Radiationrsquo Toxic Effects
Scombroid fish poisoning (often called histamine
poisoning) is caused by ingestion of certain species
of marine fish that contain high levels of histamine
and possibly other biogenic amines These fish
species contain high levels of free histidine in their
tissue and when such fish are subjected to
temperature abuse after harvest bacteria
decarboxylate histidine to produce histamine A
number of bacterial species can produce histamine
but Morganella morganii and Proteus spp are
particularly strong histamine producers
httpwwwwhointfoodsafetyhistamineen
The Role of Histamine in Radiationrsquo Toxic Effects
Eating food (fish particularly) with high levels of
histamine can result in Histamine Fish Poisoning
HFP
The symptoms of HFP can be very similar to
severe allergic reactions which can happens
within minutes to hours after ingestion of toxic fish
with high levels of Histamine in tissue
Symptoms of Histamine poisoning include
A burningtingling sensation around of mouth
Rush and or swelling over the chest andor neck
The Role of Histamine in Radiationrsquo Toxic Effects
Lowering of blood pressure
Headache
Itchy skin vomiting diarrhea
Symptoms of irradiation
The prodrome (early symptoms) of Acute Radiation Syndrome typically includes nausea and vomiting headaches fatigue fever and short period of skin reddening These symptoms may occur at radiation doses as low as 35 rad (035 Gy) These symptoms are common to many illnesses (Include HFP) and may not by themselves indicate acute radiation sickness
The Role of Histamine in Radiationrsquo s Toxic Effects The role of Histamine in development of Radiationrsquo s toxic
effects remain very controversial and need to be reconsidered
1 Scientists reported that histamine and biogenic amines playing extremely active role in development patho-physiological consequences after irradiation httpwwwintechopencombookscurrent-topics-in-ionizing-radiation-researchradiation-toxins-molecular-mechanisms-of-toxicity-and-radiomimetic-properties-
Radiation Toxicity A Practical Medical Guide
edited by William Small Gayle E Woloschak
2 Scientists reported that histamine and biogenic amines donrsquot play any role in development patho-physiological consequences and clinical signs after irradiation
The Role of Histamine in Radiationrsquo Toxic Effects 3 Scientists reported that histamine and biogenic amines
playing extremely active role in development Radioprotective mechanisms after irradiation
ldquoHistamine prevents radiation-induced toxicity by increasing proliferation of damaged intestinal mucosa and suppressing apoptosis that was associated with an increase in SOD and Catalase levels This effect might be of clinical value in patients undergoing radiotherapyrdquo Int J Radiat Biol 2007 Oct83(10)653-63
Mechanisms underlying the radioprotective effect of histamine on small intestine
Medina VA1 Croci M Mohamad NA Massari N GarbarinoG Cricco GP Nuacutentildeez MA Martiacuten GA Crescenti EJ BergocRM Rivera ES
The Role of Histamine in Radiationrsquo Toxic Effects Recommended Histamine ELISA Test Kit
Neogenrsquos Histamine ELISA test kit (Life Science Format) is a competitive direct ELISA (Enzyme-Linked ImmunosorbentAssay) in a microwell format that allows users to obtain exact concentrations of histamine in nanograms per milliliter
This kit is designed for in vitro quantification of histamine in various biological fluids by competitive direct enzyme-linked immunosorbent assay (CD-ELISA) This kit is intended for use in investigative research only and not for human clinical diagnostic use
This Histamine ELISA kit (Life Science Format) should not be used for determining histamine levels in scombroid fish Neogenoffers a separate kit for determination of histamine in fish and other foods
Intended User Researchers in biomedical fields
For Research Use Only
The Role of Histamine in Radiationrsquo Toxic Effects
Kit Contents
Histamine Antibody-Coated Microplate (96 well)
Histamine Standards (Ready-to-use)
Histamine Enzyme Conjugate (HRP)
K-Blue Substrate (TMB)
Wash Buffer (25x)
PBS Sample Diluent
Instruction Booklet
The Role of Histamine in Radiationrsquo Toxic Effects
Cross-reactivity
Histamine 10000
Histidine 0008
Cadaverine 0003
Tyramine lt001
Spermine lt001
Putrescine lt001
Trimethylamine lt001
The Role of Histamine in Radiationrsquo Toxic Effects
Specifications
Sensitivity70 BB0 25 ngmL30 BB0 10 ngmL
Assay Range 25 ndash 500 ngmLInter-Assay CV le10 Intra-Assay CV le10 Size 96 well microplateAssay Sample Size 50 μL Total Assay Incubation Time 15 hours Wavelength 650 nm 450 nm with acid stop Storage Conditions 4degC Antibody Monoclonal
The Role of Histamine in Radiationrsquo Toxic Effects
Recommended
For more information please contact us at
Neogen Corporation
Life Science
944 Nandino Boulevard
Lexington KY 40511 USA
800477-8201 (USACanada)
859254-1221
Fax 859255-5532
informneogencom
The Role of Histamine in Radiationrsquo Toxic Effects
Histamine playing important role in food toxicity
ldquoHistamine is an organic nitrogen compound that may trigger an allergic response in human and mammalian systems Other biogenic amines (such as cadaverine and putrescine) are thought to potentiate the toxic effect of histamine Although fatal cases are rare severity of the symptoms can vary depending on the amount of histamine and other biogenic amines ingested and the individualrsquos sensitivity to specific biogenic aminesrdquo WHO httpwwwwhointfoodsafetyhistamineen
The Role of Histamine in Radiationrsquo Toxic Effects
Scombroid fish poisoning (often called histamine
poisoning) is caused by ingestion of certain species
of marine fish that contain high levels of histamine
and possibly other biogenic amines These fish
species contain high levels of free histidine in their
tissue and when such fish are subjected to
temperature abuse after harvest bacteria
decarboxylate histidine to produce histamine A
number of bacterial species can produce histamine
but Morganella morganii and Proteus spp are
particularly strong histamine producers
httpwwwwhointfoodsafetyhistamineen
The Role of Histamine in Radiationrsquo Toxic Effects
Eating food (fish particularly) with high levels of
histamine can result in Histamine Fish Poisoning
HFP
The symptoms of HFP can be very similar to
severe allergic reactions which can happens
within minutes to hours after ingestion of toxic fish
with high levels of Histamine in tissue
Symptoms of Histamine poisoning include
A burningtingling sensation around of mouth
Rush and or swelling over the chest andor neck
The Role of Histamine in Radiationrsquo Toxic Effects
Lowering of blood pressure
Headache
Itchy skin vomiting diarrhea
Symptoms of irradiation
The prodrome (early symptoms) of Acute Radiation Syndrome typically includes nausea and vomiting headaches fatigue fever and short period of skin reddening These symptoms may occur at radiation doses as low as 35 rad (035 Gy) These symptoms are common to many illnesses (Include HFP) and may not by themselves indicate acute radiation sickness
The Role of Histamine in Radiationrsquo s Toxic Effects The role of Histamine in development of Radiationrsquo s toxic
effects remain very controversial and need to be reconsidered
1 Scientists reported that histamine and biogenic amines playing extremely active role in development patho-physiological consequences after irradiation httpwwwintechopencombookscurrent-topics-in-ionizing-radiation-researchradiation-toxins-molecular-mechanisms-of-toxicity-and-radiomimetic-properties-
Radiation Toxicity A Practical Medical Guide
edited by William Small Gayle E Woloschak
2 Scientists reported that histamine and biogenic amines donrsquot play any role in development patho-physiological consequences and clinical signs after irradiation
The Role of Histamine in Radiationrsquo Toxic Effects 3 Scientists reported that histamine and biogenic amines
playing extremely active role in development Radioprotective mechanisms after irradiation
ldquoHistamine prevents radiation-induced toxicity by increasing proliferation of damaged intestinal mucosa and suppressing apoptosis that was associated with an increase in SOD and Catalase levels This effect might be of clinical value in patients undergoing radiotherapyrdquo Int J Radiat Biol 2007 Oct83(10)653-63
Mechanisms underlying the radioprotective effect of histamine on small intestine
Medina VA1 Croci M Mohamad NA Massari N GarbarinoG Cricco GP Nuacutentildeez MA Martiacuten GA Crescenti EJ BergocRM Rivera ES
The Role of Histamine in Radiationrsquo Toxic Effects Recommended Histamine ELISA Test Kit
Neogenrsquos Histamine ELISA test kit (Life Science Format) is a competitive direct ELISA (Enzyme-Linked ImmunosorbentAssay) in a microwell format that allows users to obtain exact concentrations of histamine in nanograms per milliliter
This kit is designed for in vitro quantification of histamine in various biological fluids by competitive direct enzyme-linked immunosorbent assay (CD-ELISA) This kit is intended for use in investigative research only and not for human clinical diagnostic use
This Histamine ELISA kit (Life Science Format) should not be used for determining histamine levels in scombroid fish Neogenoffers a separate kit for determination of histamine in fish and other foods
Intended User Researchers in biomedical fields
For Research Use Only
The Role of Histamine in Radiationrsquo Toxic Effects
Kit Contents
Histamine Antibody-Coated Microplate (96 well)
Histamine Standards (Ready-to-use)
Histamine Enzyme Conjugate (HRP)
K-Blue Substrate (TMB)
Wash Buffer (25x)
PBS Sample Diluent
Instruction Booklet
The Role of Histamine in Radiationrsquo Toxic Effects
Cross-reactivity
Histamine 10000
Histidine 0008
Cadaverine 0003
Tyramine lt001
Spermine lt001
Putrescine lt001
Trimethylamine lt001
The Role of Histamine in Radiationrsquo Toxic Effects
Specifications
Sensitivity70 BB0 25 ngmL30 BB0 10 ngmL
Assay Range 25 ndash 500 ngmLInter-Assay CV le10 Intra-Assay CV le10 Size 96 well microplateAssay Sample Size 50 μL Total Assay Incubation Time 15 hours Wavelength 650 nm 450 nm with acid stop Storage Conditions 4degC Antibody Monoclonal
The Role of Histamine in Radiationrsquo Toxic Effects
Recommended
For more information please contact us at
Neogen Corporation
Life Science
944 Nandino Boulevard
Lexington KY 40511 USA
800477-8201 (USACanada)
859254-1221
Fax 859255-5532
informneogencom
The Role of Histamine in Radiationrsquo Toxic Effects
Scombroid fish poisoning (often called histamine
poisoning) is caused by ingestion of certain species
of marine fish that contain high levels of histamine
and possibly other biogenic amines These fish
species contain high levels of free histidine in their
tissue and when such fish are subjected to
temperature abuse after harvest bacteria
decarboxylate histidine to produce histamine A
number of bacterial species can produce histamine
but Morganella morganii and Proteus spp are
particularly strong histamine producers
httpwwwwhointfoodsafetyhistamineen
The Role of Histamine in Radiationrsquo Toxic Effects
Eating food (fish particularly) with high levels of
histamine can result in Histamine Fish Poisoning
HFP
The symptoms of HFP can be very similar to
severe allergic reactions which can happens
within minutes to hours after ingestion of toxic fish
with high levels of Histamine in tissue
Symptoms of Histamine poisoning include
A burningtingling sensation around of mouth
Rush and or swelling over the chest andor neck
The Role of Histamine in Radiationrsquo Toxic Effects
Lowering of blood pressure
Headache
Itchy skin vomiting diarrhea
Symptoms of irradiation
The prodrome (early symptoms) of Acute Radiation Syndrome typically includes nausea and vomiting headaches fatigue fever and short period of skin reddening These symptoms may occur at radiation doses as low as 35 rad (035 Gy) These symptoms are common to many illnesses (Include HFP) and may not by themselves indicate acute radiation sickness
The Role of Histamine in Radiationrsquo s Toxic Effects The role of Histamine in development of Radiationrsquo s toxic
effects remain very controversial and need to be reconsidered
1 Scientists reported that histamine and biogenic amines playing extremely active role in development patho-physiological consequences after irradiation httpwwwintechopencombookscurrent-topics-in-ionizing-radiation-researchradiation-toxins-molecular-mechanisms-of-toxicity-and-radiomimetic-properties-
Radiation Toxicity A Practical Medical Guide
edited by William Small Gayle E Woloschak
2 Scientists reported that histamine and biogenic amines donrsquot play any role in development patho-physiological consequences and clinical signs after irradiation
The Role of Histamine in Radiationrsquo Toxic Effects 3 Scientists reported that histamine and biogenic amines
playing extremely active role in development Radioprotective mechanisms after irradiation
ldquoHistamine prevents radiation-induced toxicity by increasing proliferation of damaged intestinal mucosa and suppressing apoptosis that was associated with an increase in SOD and Catalase levels This effect might be of clinical value in patients undergoing radiotherapyrdquo Int J Radiat Biol 2007 Oct83(10)653-63
Mechanisms underlying the radioprotective effect of histamine on small intestine
Medina VA1 Croci M Mohamad NA Massari N GarbarinoG Cricco GP Nuacutentildeez MA Martiacuten GA Crescenti EJ BergocRM Rivera ES
The Role of Histamine in Radiationrsquo Toxic Effects Recommended Histamine ELISA Test Kit
Neogenrsquos Histamine ELISA test kit (Life Science Format) is a competitive direct ELISA (Enzyme-Linked ImmunosorbentAssay) in a microwell format that allows users to obtain exact concentrations of histamine in nanograms per milliliter
This kit is designed for in vitro quantification of histamine in various biological fluids by competitive direct enzyme-linked immunosorbent assay (CD-ELISA) This kit is intended for use in investigative research only and not for human clinical diagnostic use
This Histamine ELISA kit (Life Science Format) should not be used for determining histamine levels in scombroid fish Neogenoffers a separate kit for determination of histamine in fish and other foods
Intended User Researchers in biomedical fields
For Research Use Only
The Role of Histamine in Radiationrsquo Toxic Effects
Kit Contents
Histamine Antibody-Coated Microplate (96 well)
Histamine Standards (Ready-to-use)
Histamine Enzyme Conjugate (HRP)
K-Blue Substrate (TMB)
Wash Buffer (25x)
PBS Sample Diluent
Instruction Booklet
The Role of Histamine in Radiationrsquo Toxic Effects
Cross-reactivity
Histamine 10000
Histidine 0008
Cadaverine 0003
Tyramine lt001
Spermine lt001
Putrescine lt001
Trimethylamine lt001
The Role of Histamine in Radiationrsquo Toxic Effects
Specifications
Sensitivity70 BB0 25 ngmL30 BB0 10 ngmL
Assay Range 25 ndash 500 ngmLInter-Assay CV le10 Intra-Assay CV le10 Size 96 well microplateAssay Sample Size 50 μL Total Assay Incubation Time 15 hours Wavelength 650 nm 450 nm with acid stop Storage Conditions 4degC Antibody Monoclonal
The Role of Histamine in Radiationrsquo Toxic Effects
Recommended
For more information please contact us at
Neogen Corporation
Life Science
944 Nandino Boulevard
Lexington KY 40511 USA
800477-8201 (USACanada)
859254-1221
Fax 859255-5532
informneogencom
The Role of Histamine in Radiationrsquo Toxic Effects
Eating food (fish particularly) with high levels of
histamine can result in Histamine Fish Poisoning
HFP
The symptoms of HFP can be very similar to
severe allergic reactions which can happens
within minutes to hours after ingestion of toxic fish
with high levels of Histamine in tissue
Symptoms of Histamine poisoning include
A burningtingling sensation around of mouth
Rush and or swelling over the chest andor neck
The Role of Histamine in Radiationrsquo Toxic Effects
Lowering of blood pressure
Headache
Itchy skin vomiting diarrhea
Symptoms of irradiation
The prodrome (early symptoms) of Acute Radiation Syndrome typically includes nausea and vomiting headaches fatigue fever and short period of skin reddening These symptoms may occur at radiation doses as low as 35 rad (035 Gy) These symptoms are common to many illnesses (Include HFP) and may not by themselves indicate acute radiation sickness
The Role of Histamine in Radiationrsquo s Toxic Effects The role of Histamine in development of Radiationrsquo s toxic
effects remain very controversial and need to be reconsidered
1 Scientists reported that histamine and biogenic amines playing extremely active role in development patho-physiological consequences after irradiation httpwwwintechopencombookscurrent-topics-in-ionizing-radiation-researchradiation-toxins-molecular-mechanisms-of-toxicity-and-radiomimetic-properties-
Radiation Toxicity A Practical Medical Guide
edited by William Small Gayle E Woloschak
2 Scientists reported that histamine and biogenic amines donrsquot play any role in development patho-physiological consequences and clinical signs after irradiation
The Role of Histamine in Radiationrsquo Toxic Effects 3 Scientists reported that histamine and biogenic amines
playing extremely active role in development Radioprotective mechanisms after irradiation
ldquoHistamine prevents radiation-induced toxicity by increasing proliferation of damaged intestinal mucosa and suppressing apoptosis that was associated with an increase in SOD and Catalase levels This effect might be of clinical value in patients undergoing radiotherapyrdquo Int J Radiat Biol 2007 Oct83(10)653-63
Mechanisms underlying the radioprotective effect of histamine on small intestine
Medina VA1 Croci M Mohamad NA Massari N GarbarinoG Cricco GP Nuacutentildeez MA Martiacuten GA Crescenti EJ BergocRM Rivera ES
The Role of Histamine in Radiationrsquo Toxic Effects Recommended Histamine ELISA Test Kit
Neogenrsquos Histamine ELISA test kit (Life Science Format) is a competitive direct ELISA (Enzyme-Linked ImmunosorbentAssay) in a microwell format that allows users to obtain exact concentrations of histamine in nanograms per milliliter
This kit is designed for in vitro quantification of histamine in various biological fluids by competitive direct enzyme-linked immunosorbent assay (CD-ELISA) This kit is intended for use in investigative research only and not for human clinical diagnostic use
This Histamine ELISA kit (Life Science Format) should not be used for determining histamine levels in scombroid fish Neogenoffers a separate kit for determination of histamine in fish and other foods
Intended User Researchers in biomedical fields
For Research Use Only
The Role of Histamine in Radiationrsquo Toxic Effects
Kit Contents
Histamine Antibody-Coated Microplate (96 well)
Histamine Standards (Ready-to-use)
Histamine Enzyme Conjugate (HRP)
K-Blue Substrate (TMB)
Wash Buffer (25x)
PBS Sample Diluent
Instruction Booklet
The Role of Histamine in Radiationrsquo Toxic Effects
Cross-reactivity
Histamine 10000
Histidine 0008
Cadaverine 0003
Tyramine lt001
Spermine lt001
Putrescine lt001
Trimethylamine lt001
The Role of Histamine in Radiationrsquo Toxic Effects
Specifications
Sensitivity70 BB0 25 ngmL30 BB0 10 ngmL
Assay Range 25 ndash 500 ngmLInter-Assay CV le10 Intra-Assay CV le10 Size 96 well microplateAssay Sample Size 50 μL Total Assay Incubation Time 15 hours Wavelength 650 nm 450 nm with acid stop Storage Conditions 4degC Antibody Monoclonal
The Role of Histamine in Radiationrsquo Toxic Effects
Recommended
For more information please contact us at
Neogen Corporation
Life Science
944 Nandino Boulevard
Lexington KY 40511 USA
800477-8201 (USACanada)
859254-1221
Fax 859255-5532
informneogencom
The Role of Histamine in Radiationrsquo Toxic Effects
Lowering of blood pressure
Headache
Itchy skin vomiting diarrhea
Symptoms of irradiation
The prodrome (early symptoms) of Acute Radiation Syndrome typically includes nausea and vomiting headaches fatigue fever and short period of skin reddening These symptoms may occur at radiation doses as low as 35 rad (035 Gy) These symptoms are common to many illnesses (Include HFP) and may not by themselves indicate acute radiation sickness
The Role of Histamine in Radiationrsquo s Toxic Effects The role of Histamine in development of Radiationrsquo s toxic
effects remain very controversial and need to be reconsidered
1 Scientists reported that histamine and biogenic amines playing extremely active role in development patho-physiological consequences after irradiation httpwwwintechopencombookscurrent-topics-in-ionizing-radiation-researchradiation-toxins-molecular-mechanisms-of-toxicity-and-radiomimetic-properties-
Radiation Toxicity A Practical Medical Guide
edited by William Small Gayle E Woloschak
2 Scientists reported that histamine and biogenic amines donrsquot play any role in development patho-physiological consequences and clinical signs after irradiation
The Role of Histamine in Radiationrsquo Toxic Effects 3 Scientists reported that histamine and biogenic amines
playing extremely active role in development Radioprotective mechanisms after irradiation
ldquoHistamine prevents radiation-induced toxicity by increasing proliferation of damaged intestinal mucosa and suppressing apoptosis that was associated with an increase in SOD and Catalase levels This effect might be of clinical value in patients undergoing radiotherapyrdquo Int J Radiat Biol 2007 Oct83(10)653-63
Mechanisms underlying the radioprotective effect of histamine on small intestine
Medina VA1 Croci M Mohamad NA Massari N GarbarinoG Cricco GP Nuacutentildeez MA Martiacuten GA Crescenti EJ BergocRM Rivera ES
The Role of Histamine in Radiationrsquo Toxic Effects Recommended Histamine ELISA Test Kit
Neogenrsquos Histamine ELISA test kit (Life Science Format) is a competitive direct ELISA (Enzyme-Linked ImmunosorbentAssay) in a microwell format that allows users to obtain exact concentrations of histamine in nanograms per milliliter
This kit is designed for in vitro quantification of histamine in various biological fluids by competitive direct enzyme-linked immunosorbent assay (CD-ELISA) This kit is intended for use in investigative research only and not for human clinical diagnostic use
This Histamine ELISA kit (Life Science Format) should not be used for determining histamine levels in scombroid fish Neogenoffers a separate kit for determination of histamine in fish and other foods
Intended User Researchers in biomedical fields
For Research Use Only
The Role of Histamine in Radiationrsquo Toxic Effects
Kit Contents
Histamine Antibody-Coated Microplate (96 well)
Histamine Standards (Ready-to-use)
Histamine Enzyme Conjugate (HRP)
K-Blue Substrate (TMB)
Wash Buffer (25x)
PBS Sample Diluent
Instruction Booklet
The Role of Histamine in Radiationrsquo Toxic Effects
Cross-reactivity
Histamine 10000
Histidine 0008
Cadaverine 0003
Tyramine lt001
Spermine lt001
Putrescine lt001
Trimethylamine lt001
The Role of Histamine in Radiationrsquo Toxic Effects
Specifications
Sensitivity70 BB0 25 ngmL30 BB0 10 ngmL
Assay Range 25 ndash 500 ngmLInter-Assay CV le10 Intra-Assay CV le10 Size 96 well microplateAssay Sample Size 50 μL Total Assay Incubation Time 15 hours Wavelength 650 nm 450 nm with acid stop Storage Conditions 4degC Antibody Monoclonal
The Role of Histamine in Radiationrsquo Toxic Effects
Recommended
For more information please contact us at
Neogen Corporation
Life Science
944 Nandino Boulevard
Lexington KY 40511 USA
800477-8201 (USACanada)
859254-1221
Fax 859255-5532
informneogencom
The Role of Histamine in Radiationrsquo s Toxic Effects The role of Histamine in development of Radiationrsquo s toxic
effects remain very controversial and need to be reconsidered
1 Scientists reported that histamine and biogenic amines playing extremely active role in development patho-physiological consequences after irradiation httpwwwintechopencombookscurrent-topics-in-ionizing-radiation-researchradiation-toxins-molecular-mechanisms-of-toxicity-and-radiomimetic-properties-
Radiation Toxicity A Practical Medical Guide
edited by William Small Gayle E Woloschak
2 Scientists reported that histamine and biogenic amines donrsquot play any role in development patho-physiological consequences and clinical signs after irradiation
The Role of Histamine in Radiationrsquo Toxic Effects 3 Scientists reported that histamine and biogenic amines
playing extremely active role in development Radioprotective mechanisms after irradiation
ldquoHistamine prevents radiation-induced toxicity by increasing proliferation of damaged intestinal mucosa and suppressing apoptosis that was associated with an increase in SOD and Catalase levels This effect might be of clinical value in patients undergoing radiotherapyrdquo Int J Radiat Biol 2007 Oct83(10)653-63
Mechanisms underlying the radioprotective effect of histamine on small intestine
Medina VA1 Croci M Mohamad NA Massari N GarbarinoG Cricco GP Nuacutentildeez MA Martiacuten GA Crescenti EJ BergocRM Rivera ES
The Role of Histamine in Radiationrsquo Toxic Effects Recommended Histamine ELISA Test Kit
Neogenrsquos Histamine ELISA test kit (Life Science Format) is a competitive direct ELISA (Enzyme-Linked ImmunosorbentAssay) in a microwell format that allows users to obtain exact concentrations of histamine in nanograms per milliliter
This kit is designed for in vitro quantification of histamine in various biological fluids by competitive direct enzyme-linked immunosorbent assay (CD-ELISA) This kit is intended for use in investigative research only and not for human clinical diagnostic use
This Histamine ELISA kit (Life Science Format) should not be used for determining histamine levels in scombroid fish Neogenoffers a separate kit for determination of histamine in fish and other foods
Intended User Researchers in biomedical fields
For Research Use Only
The Role of Histamine in Radiationrsquo Toxic Effects
Kit Contents
Histamine Antibody-Coated Microplate (96 well)
Histamine Standards (Ready-to-use)
Histamine Enzyme Conjugate (HRP)
K-Blue Substrate (TMB)
Wash Buffer (25x)
PBS Sample Diluent
Instruction Booklet
The Role of Histamine in Radiationrsquo Toxic Effects
Cross-reactivity
Histamine 10000
Histidine 0008
Cadaverine 0003
Tyramine lt001
Spermine lt001
Putrescine lt001
Trimethylamine lt001
The Role of Histamine in Radiationrsquo Toxic Effects
Specifications
Sensitivity70 BB0 25 ngmL30 BB0 10 ngmL
Assay Range 25 ndash 500 ngmLInter-Assay CV le10 Intra-Assay CV le10 Size 96 well microplateAssay Sample Size 50 μL Total Assay Incubation Time 15 hours Wavelength 650 nm 450 nm with acid stop Storage Conditions 4degC Antibody Monoclonal
The Role of Histamine in Radiationrsquo Toxic Effects
Recommended
For more information please contact us at
Neogen Corporation
Life Science
944 Nandino Boulevard
Lexington KY 40511 USA
800477-8201 (USACanada)
859254-1221
Fax 859255-5532
informneogencom
The Role of Histamine in Radiationrsquo Toxic Effects 3 Scientists reported that histamine and biogenic amines
playing extremely active role in development Radioprotective mechanisms after irradiation
ldquoHistamine prevents radiation-induced toxicity by increasing proliferation of damaged intestinal mucosa and suppressing apoptosis that was associated with an increase in SOD and Catalase levels This effect might be of clinical value in patients undergoing radiotherapyrdquo Int J Radiat Biol 2007 Oct83(10)653-63
Mechanisms underlying the radioprotective effect of histamine on small intestine
Medina VA1 Croci M Mohamad NA Massari N GarbarinoG Cricco GP Nuacutentildeez MA Martiacuten GA Crescenti EJ BergocRM Rivera ES
The Role of Histamine in Radiationrsquo Toxic Effects Recommended Histamine ELISA Test Kit
Neogenrsquos Histamine ELISA test kit (Life Science Format) is a competitive direct ELISA (Enzyme-Linked ImmunosorbentAssay) in a microwell format that allows users to obtain exact concentrations of histamine in nanograms per milliliter
This kit is designed for in vitro quantification of histamine in various biological fluids by competitive direct enzyme-linked immunosorbent assay (CD-ELISA) This kit is intended for use in investigative research only and not for human clinical diagnostic use
This Histamine ELISA kit (Life Science Format) should not be used for determining histamine levels in scombroid fish Neogenoffers a separate kit for determination of histamine in fish and other foods
Intended User Researchers in biomedical fields
For Research Use Only
The Role of Histamine in Radiationrsquo Toxic Effects
Kit Contents
Histamine Antibody-Coated Microplate (96 well)
Histamine Standards (Ready-to-use)
Histamine Enzyme Conjugate (HRP)
K-Blue Substrate (TMB)
Wash Buffer (25x)
PBS Sample Diluent
Instruction Booklet
The Role of Histamine in Radiationrsquo Toxic Effects
Cross-reactivity
Histamine 10000
Histidine 0008
Cadaverine 0003
Tyramine lt001
Spermine lt001
Putrescine lt001
Trimethylamine lt001
The Role of Histamine in Radiationrsquo Toxic Effects
Specifications
Sensitivity70 BB0 25 ngmL30 BB0 10 ngmL
Assay Range 25 ndash 500 ngmLInter-Assay CV le10 Intra-Assay CV le10 Size 96 well microplateAssay Sample Size 50 μL Total Assay Incubation Time 15 hours Wavelength 650 nm 450 nm with acid stop Storage Conditions 4degC Antibody Monoclonal
The Role of Histamine in Radiationrsquo Toxic Effects
Recommended
For more information please contact us at
Neogen Corporation
Life Science
944 Nandino Boulevard
Lexington KY 40511 USA
800477-8201 (USACanada)
859254-1221
Fax 859255-5532
informneogencom
The Role of Histamine in Radiationrsquo Toxic Effects Recommended Histamine ELISA Test Kit
Neogenrsquos Histamine ELISA test kit (Life Science Format) is a competitive direct ELISA (Enzyme-Linked ImmunosorbentAssay) in a microwell format that allows users to obtain exact concentrations of histamine in nanograms per milliliter
This kit is designed for in vitro quantification of histamine in various biological fluids by competitive direct enzyme-linked immunosorbent assay (CD-ELISA) This kit is intended for use in investigative research only and not for human clinical diagnostic use
This Histamine ELISA kit (Life Science Format) should not be used for determining histamine levels in scombroid fish Neogenoffers a separate kit for determination of histamine in fish and other foods
Intended User Researchers in biomedical fields
For Research Use Only
The Role of Histamine in Radiationrsquo Toxic Effects
Kit Contents
Histamine Antibody-Coated Microplate (96 well)
Histamine Standards (Ready-to-use)
Histamine Enzyme Conjugate (HRP)
K-Blue Substrate (TMB)
Wash Buffer (25x)
PBS Sample Diluent
Instruction Booklet
The Role of Histamine in Radiationrsquo Toxic Effects
Cross-reactivity
Histamine 10000
Histidine 0008
Cadaverine 0003
Tyramine lt001
Spermine lt001
Putrescine lt001
Trimethylamine lt001
The Role of Histamine in Radiationrsquo Toxic Effects
Specifications
Sensitivity70 BB0 25 ngmL30 BB0 10 ngmL
Assay Range 25 ndash 500 ngmLInter-Assay CV le10 Intra-Assay CV le10 Size 96 well microplateAssay Sample Size 50 μL Total Assay Incubation Time 15 hours Wavelength 650 nm 450 nm with acid stop Storage Conditions 4degC Antibody Monoclonal
The Role of Histamine in Radiationrsquo Toxic Effects
Recommended
For more information please contact us at
Neogen Corporation
Life Science
944 Nandino Boulevard
Lexington KY 40511 USA
800477-8201 (USACanada)
859254-1221
Fax 859255-5532
informneogencom
The Role of Histamine in Radiationrsquo Toxic Effects
Kit Contents
Histamine Antibody-Coated Microplate (96 well)
Histamine Standards (Ready-to-use)
Histamine Enzyme Conjugate (HRP)
K-Blue Substrate (TMB)
Wash Buffer (25x)
PBS Sample Diluent
Instruction Booklet
The Role of Histamine in Radiationrsquo Toxic Effects
Cross-reactivity
Histamine 10000
Histidine 0008
Cadaverine 0003
Tyramine lt001
Spermine lt001
Putrescine lt001
Trimethylamine lt001
The Role of Histamine in Radiationrsquo Toxic Effects
Specifications
Sensitivity70 BB0 25 ngmL30 BB0 10 ngmL
Assay Range 25 ndash 500 ngmLInter-Assay CV le10 Intra-Assay CV le10 Size 96 well microplateAssay Sample Size 50 μL Total Assay Incubation Time 15 hours Wavelength 650 nm 450 nm with acid stop Storage Conditions 4degC Antibody Monoclonal
The Role of Histamine in Radiationrsquo Toxic Effects
Recommended
For more information please contact us at
Neogen Corporation
Life Science
944 Nandino Boulevard
Lexington KY 40511 USA
800477-8201 (USACanada)
859254-1221
Fax 859255-5532
informneogencom
The Role of Histamine in Radiationrsquo Toxic Effects
Cross-reactivity
Histamine 10000
Histidine 0008
Cadaverine 0003
Tyramine lt001
Spermine lt001
Putrescine lt001
Trimethylamine lt001
The Role of Histamine in Radiationrsquo Toxic Effects
Specifications
Sensitivity70 BB0 25 ngmL30 BB0 10 ngmL
Assay Range 25 ndash 500 ngmLInter-Assay CV le10 Intra-Assay CV le10 Size 96 well microplateAssay Sample Size 50 μL Total Assay Incubation Time 15 hours Wavelength 650 nm 450 nm with acid stop Storage Conditions 4degC Antibody Monoclonal
The Role of Histamine in Radiationrsquo Toxic Effects
Recommended
For more information please contact us at
Neogen Corporation
Life Science
944 Nandino Boulevard
Lexington KY 40511 USA
800477-8201 (USACanada)
859254-1221
Fax 859255-5532
informneogencom
The Role of Histamine in Radiationrsquo Toxic Effects
Specifications
Sensitivity70 BB0 25 ngmL30 BB0 10 ngmL
Assay Range 25 ndash 500 ngmLInter-Assay CV le10 Intra-Assay CV le10 Size 96 well microplateAssay Sample Size 50 μL Total Assay Incubation Time 15 hours Wavelength 650 nm 450 nm with acid stop Storage Conditions 4degC Antibody Monoclonal
The Role of Histamine in Radiationrsquo Toxic Effects
Recommended
For more information please contact us at
Neogen Corporation
Life Science
944 Nandino Boulevard
Lexington KY 40511 USA
800477-8201 (USACanada)
859254-1221
Fax 859255-5532
informneogencom
The Role of Histamine in Radiationrsquo Toxic Effects
Recommended
For more information please contact us at
Neogen Corporation
Life Science
944 Nandino Boulevard
Lexington KY 40511 USA
800477-8201 (USACanada)
859254-1221
Fax 859255-5532
informneogencom
