The Project PICTA-KILL - Novel Strategies for Biological ... · The project PICTA-KILL - Novel...
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The project PICTA-KILL - Novel strategies for biological psyllid pest control Linda Muskat 1 , Pascal Humbert 1 , Jürgen Gross 2 , Louisa Maria Görg 2 , Elisa Beitzen-Heineke 3 , Wilhelm Beitzen-Heineke 3 , Michael Przyklenk 3 , Cornelia Dippel 4 , Annette H. Jensen 5 , Annette B. Jensen 5 , Jørgen Eilenberg 5 , Anant V. Patel 1 1 University of Applied Sciences, Department of Engineering and Mathematics, Fermentation and Formulation of Biologicals and Chemicals, Bielefeld, Germany; 2 Julius Kühn-Institut Dossenheim, Germany; 3 BIOCARE Gesellschaft für biologische Schutzmittel mbH, Einbeck, Germany, 4 IS Insect Services GmbH, Berlin, Germany; 5 University of Copenhagen, Denmark TROPENTAG 2017, Bonn, Germany Contact Linda Muskat FH Bielefeld; Interaktion 1, 33619 Bielefeld WG Fermentation and Formulation of biologicals and chemicals [email protected] Acknowledgements The project is supported by funds of the Federal Ministry of Food, Agriculture and Consumer Protection (BMELV) based on a decision of the Parliament of the Federal Republic of Germany via the Federal Office for Agriculture and Food (BLE) under the innovation support program. Candidatus Phytoplasma mali https://de.wikipedia.org/wiki/A pfeltriebsucht#/media/File:Apfel triebsucht.jpg https://idw-online.de/de/image?id=96770&size=screen http://www.frontiersin.org/files/Articles/61362/fmicb-04- 00230-HTML/image_m/fmicb-04-00230-g001.jpg Apple proliferation Cacopsylla picta Psyllid pests are distributed all over the world and cause damage in crop plants. Novel defense strategies against these insect pests are of international interest. Being the vector of Candidatus Phytoplasma mali, the infectious agent of apple proliferation, the psyllid Cacopsylla picta (Hemiptera; Psylloidea) is responsible for an annual economic loss of a three-digit-million range in Europe. Because there are no direct measures to combat apple proliferation, the vector itself has to be controlled in order to protect the plants. DISEASE PATHOGEN VECTOR The overall aim of this recently granted project is to develop semiochemical- based co-formulations, which can be applied for “Attract-and-Kill”-strategies against C. picta. Furthermore, it will be examined if a combination with repellent agents supports the effect (“Push-Pull-Kill”- strategy). INTRODUCTION • An entomopathogenic fungus of the genus Pandora sp. (Entomophthorales) was isolated from the target insect Cacopsylla sp. • The pathogenicity of the isolated fungus was demonstrated for different Cacopsylla species • The fungus can be formulated within Ca-alginate beads and nutrients. • An computer-assisted evaluation method was developed for the quantification of discharged conidia PROJECT STRUCTURE Entomopathogenic fungi • Pandora sp. (Entomophthorales) (UC) • Metarhizium sp. • Beauveria sp. Insecticides • natural • synthetic REPEL/PUSH ATTRACT/PULL KILL FORMULATION Efficacy tests under laboratory conditions (JKI, FHB, UC) Cultivation (FHB) Screening for formulation materials and methods (FHB) Polymers and adjuvants Methods for the production of (micro-)capsules applicable within a spray suspension or in combination with a trap Potential Repellents • benzylalcohol • bornylacetate • mixtures Potential Attractants • β-caryophyllene • ethylbenzoate • mixtures Screening of attracting, repelling and arresting semiochemicals under laboratory conditions(JKI, IS) Collection and rearing of insects (JKI, IS) Analysis of emission rate of semiochemicals from formulations (FHB, JKI) Efficacy evaluation under laboratory conditions (IS, JKI) Scale-up (BIO, FHB) Efficacy evaluation under field conditions (JKI) Repel/Push formulations Collection and rearing of insects (IS, JKI) Mass production (FHB, BIO) Formulation (FHB) Collection and rearing of insects (IS, JKI) Compounds efficiently killing C. picta under laboratory conditions Attract/Pull formulations Attract-and-kill formulations Kill formulations Identified repellents Identified attractants Optimized formulations that with attract-and-kill or push-pull-kill strategies show high efficacy in the field FIRST RESULTS 1 cm 2 UC: University of Copenhagen JKI: Julius Kühn-Institut BIO: BIOCARE IS: Insect Services FHB: FH Bielefeld REPEL ATTRACT KILL
Transcript of The Project PICTA-KILL - Novel Strategies for Biological ... · The project PICTA-KILL - Novel...
The project PICTA-KILL - Novel strategies for biological psyllid pest control
Linda Muskat1, Pascal Humbert1, Jürgen Gross2, Louisa Maria Görg2, Elisa Beitzen-Heineke3, Wilhelm Beitzen-Heineke3, Michael Przyklenk3, Cornelia Dippel4, Annette H. Jensen5, Annette B. Jensen5, Jørgen Eilenberg5, Anant V. Patel1
1 University of Applied Sciences, Department of Engineering and Mathematics, Fermentation and Formulation of Biologicals and Chemicals, Bielefeld, Germany;2 Julius Kühn-Institut Dossenheim, Germany; 3 BIOCARE Gesellschaft für biologische Schutzmittel mbH, Einbeck, Germany, 4 IS Insect Services GmbH, Berlin, Germany; 5 University of Copenhagen, Denmark
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Contact
Linda Muskat
FH Bielefeld; Interaktion 1, 33619 Bielefeld
WG Fermentation and Formulation of biologicals and chemicals
AcknowledgementsThe project is supported by funds of the Federal Ministry of Food, Agriculture and Consumer Protection (BMELV) based on a decision of the Parliament of the Federal Republic of Germany via the Federal Office for Agriculture and Food (BLE) under the innovation support program.
Candidatus Phytoplasma mali
https://de.wikipedia.org/wiki/Apfeltriebsucht#/media/File:Apfeltriebsucht.jpg
https://idw-online.de/de/image?id=96770&size=screen
http://www.frontiersin.org/files/Articles/61362/fmicb-04-00230-HTML/image_m/fmicb-04-00230-g001.jpg
Apple proliferation Cacopsylla picta
Psyllid pests are distributed all over the world and cause damage in crop plants. Novel defense strategies against these insect pests are of international interest.Being the vector of Candidatus Phytoplasma mali, the infectious agent of apple proliferation, the psyllid Cacopsylla picta (Hemiptera; Psylloidea) is responsible for an annual economic loss of a three-digit-million range in Europe. Because there are no direct measures to combat apple proliferation, the vector itself has to be controlled in order to protect the plants.
DISEASE PATHOGEN VECTOR
The overall aim of this recently granted project is to develop semiochemical-based co-formulations, which can be applied for “Attract-and-Kill”-strategies against C. picta. Furthermore, it will be examined if a combination with repellent agents supports the effect (“Push-Pull-Kill”- strategy).
INTRODUCTION
• An entomopathogenic fungus of the genus Pandora sp. (Entomophthorales) was isolatedfrom the target insect Cacopsylla sp.
• The pathogenicity of the isolated fungus was demonstrated for different Cacopsylla species• The fungus can be formulated within Ca-alginate beads and nutrients.• An computer-assisted evaluation method was developed for the quantification of discharged conidia
PROJECT STRUCTURE
Entomopathogenic fungi• Pandora sp. (Entomophthorales) (UC)• Metarhizium sp.• Beauveria sp.Insecticides• natural• synthetic
REPEL/PUSH ATTRACT/PULL KILL FORMULATION
Efficacy tests under laboratory conditions(JKI, FHB, UC)
Cultivation (FHB)
Screening for formulation materials andmethods (FHB)
Polymers and adjuvants
Methods for the production of(micro-)capsules applicable within a spray suspension or in combination
with a trap
Potential Repellents• benzylalcohol• bornylacetate• mixtures
Potential Attractants• β-caryophyllene• ethylbenzoate• mixtures
Screening of attracting, repelling and arresting semiochemicals underlaboratory conditions(JKI, IS)
Collection and rearing of insects (JKI, IS)
Analysis of emission rate of semiochemicals from formulations (FHB, JKI)
Efficacy evaluation under laboratory conditions (IS, JKI)
Scale-up (BIO, FHB)
Efficacy evaluation under field conditions (JKI)
Repel/Push formulations
Collection and rearing of insects (IS, JKI)
Mass production (FHB, BIO)
Formulation (FHB)
Collection and rearing of insects (IS, JKI)
Compounds efficiently killing C. pictaunder laboratory conditions
Attract/Pull formulations
Attract-and-kill formulations
Kill formulations
Identifiedrepellents
Identifiedattractants
Optimized formulations that with attract-and-kill or push-pull-kill strategies show high efficacy in the field
FIRST RESULTS
1 cm2
UC: University of CopenhagenJKI: Julius Kühn-InstitutBIO: BIOCAREIS: Insect ServicesFHB: FH Bielefeld
REPEL
ATTRACT
KILL
