The optimization of isolating and identifying of Fusobacterium necrophorum species involved into...
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Abstracts / Journal of Biotechnology 185S (2014) S37–S125 S93 III – left ear = 3.61, wave V – left ear = 3.15; dog 3: wave V – left ear = 3.57, right ear = 4.45). Conclusion: In congenital hydrocephalic dogs from this study, increased amplitudes of BAER waves III and V were observed. These changes are rare in BAER evaluations and can be explained by the inhibition of cortical auditory neurons or of descending auditory pathways. http://dx.doi.org/10.1016/j.jbiotec.2014.07.316 The optimization of isolating and identifying of Fusobacterium necrophorum species involved into sheep’s and cattle’s affections Laura Aneta Tudor 1,∗ , Paul Grigorescu 1 , Elena Mitranescu 2 , Laurentiu Tudor 2 1 Faculty of Veterinary Medicine “Spiru-Haret” Bucharest, Romania 2 Faculty of Veterinary Medicine, Bucharest, Romania E-mail address: [email protected] (L.A. Tudor). There have been effectuated comparatively investigations for iso- lating and identifying of Fusobacterium necrophorum species from many samples of necrotic-pus material harvested from sheep and cattle with different affections generated by this bacteria. For this scope, from the samples has been simultaneous inseminated on three different selective mediums: agar VL with horse blood, green brilliant and sodium azide; agar heart–brain with violet crys- tal, phenethyl-alcohol and yolk emulsion; agar heart–brain with horse blood, kanamycin and vancomycin. Following the effectu- ated researches, on agar VL with horse blood, green brilliant and sodium azide it has been isolated 24 F. necrophorum stems, on agar hearth-brain with violet crystal, phenethyl-alcohol and yolk emulsion it has been isolated 36 stems and 11 stems on agar heart–brain with horse blood, kanamycin and vancomycin. Based on the obtained results, we consider that the agar VL with horse blood, green brilliant and sodium azide is the most efficient for isolating and identifying the F. necrophorum species, presenting the advantage of faster growing up of colonies (48 h maximum) comparatively of agar heart–brain with violet crystal, phenethyl- alcohol and yolk emulsion that make the colonies to be visible just after 72 h of incubation. http://dx.doi.org/10.1016/j.jbiotec.2014.07.317 Detection of mechanism and anticancer activity of the new quinoline compounds MC20 and MC21* Tu˘ gba Kul Köprülü 1,∗ ,S ¸ aban Tekin 2 , Salih Ökten 3 , Merve C ¸ ınar 4 , Seda Duman 5 , Osman C ¸ akmak 4 1 Department of Biology, Gaziosmanpasa University, Faculty of Science & Art, Tokat, Turkey 2 Department of Molecular Biology & Genetics, Gaziosmanpasa University, Faculty of Science & Art, Tokat, Turkey 3 Primary Education, Division of Science Education, Faculty of Education, Kirikkale University, Kirikkale, Turkey 4 Department of Chemistry, Faculty of Art and Science, Yildiz Technical University, Davutpasa, ˙ Istanbul, Turkey 5 Departments of Chemistry, Gaziosmanpasa University, Faculty of Science & Art, Tokat, Turkey E-mail address: tugbakul [email protected] (T.K. Köprülü). Various quinoline compounds have received great attention due to their potent biological activities. The aim of this study was to investigate the anticancer properties and mechanism of action of the novel quinoline derivatives, MC20 and MC21. Antiproliferative and cytotoxic activities of MC20 and MC21 were tested on HeLa (human cervix carcinoma), HT29 (human colon carcinoma), and C6 (rat brain carcinoma) cell lines in vitro using BrdU cell prolifer- ation ELISA, sulphorhodamine B (SRB), and lactate dehydrogenase (LDH) assay. The mechanism of anticancer activity of MC20 and 21 were determined using DNA laddering assay which indicates apoptosis. Results indicated that the new quinoline derivatives, MC20 and MC21 were more antiproliferative and cytotoxic against tumor cells than anticancer drug, 5-flourourasil (5-FU). MC20 and MC21 caused DNA laddering in tumor cells in DNA laddering assays, indicating that they prevent proliferation of tumor cells by inducing apoptosis. The results showed that the MC20 and MC21 are potential anticancer drug candidate with high antiproliferative activity on tumor cells in vitro. *This study is supported by a grant (TBAG 112T394) from TUBITAK. http://dx.doi.org/10.1016/j.jbiotec.2014.07.318 Diagnostic techniques performed before and after the ablation of a tumored prostate and urinary bladder in a dog Vulpe Vasile 1,∗ , Pasca Aurelian Sorin 1 , Vulpe Cristina Alice 2 , Papuc Ionel 2 1 Department of Clinics, Veterinary Faculty, Agricultural Sciences and Veterinary Medicine, Iasi, Romania 2 Department of Doctoral Schools, Veterinary Faculty, Agricultural Sciences and Veterinary Medicine, Cluj-Napoca, Romania E-mail address: vasile [email protected] (V. Vasile). In dogs, the prostate can suffer various processes, such as hyper- plasia or malignancy. The purpose of this paper is to underline the connection between the clinical and imaging diagnostic techniques and the surgical ones, while also preserving and ensuring the life of the patient. One Pekinese 14 year old dog was examined radio-
Transcript of The optimization of isolating and identifying of Fusobacterium necrophorum species involved into...
Abstracts / Journal of Biotechnology 185S (2014) S37–S125 S93
III – left ear = 3.61, wave V – left ear = 3.15; dog 3: wave V – leftear = 3.57, right ear = 4.45). Conclusion: In congenital hydrocephalicdogs from this study, increased amplitudes of BAER waves III andV were observed. These changes are rare in BAER evaluations andcan be explained by the inhibition of cortical auditory neurons orof descending auditory pathways.
http://dx.doi.org/10.1016/j.jbiotec.2014.07.316
The optimization of isolating and identifying ofFusobacterium necrophorum species involvedinto sheep’s and cattle’s affections
Laura Aneta Tudor 1,∗, Paul Grigorescu 1, ElenaMitranescu 2, Laurentiu Tudor 2
1 Faculty of Veterinary Medicine “Spiru-Haret”Bucharest, Romania2 Faculty of Veterinary Medicine, Bucharest, Romania
E-mail address: [email protected] (L.A. Tudor).
There have been effectuated comparatively investigations for iso-lating and identifying of Fusobacterium necrophorum species frommany samples of necrotic-pus material harvested from sheep andcattle with different affections generated by this bacteria. For thisscope, from the samples has been simultaneous inseminated onthree different selective mediums: agar VL with horse blood, greenbrilliant and sodium azide; agar heart–brain with violet crys-tal, phenethyl-alcohol and yolk emulsion; agar heart–brain withhorse blood, kanamycin and vancomycin. Following the effectu-ated researches, on agar VL with horse blood, green brilliant andsodium azide it has been isolated 24 F. necrophorum stems, onagar hearth-brain with violet crystal, phenethyl-alcohol and yolkemulsion it has been isolated 36 stems and 11 stems on agarheart–brain with horse blood, kanamycin and vancomycin. Basedon the obtained results, we consider that the agar VL with horseblood, green brilliant and sodium azide is the most efficient forisolating and identifying the F. necrophorum species, presentingthe advantage of faster growing up of colonies (48 h maximum)comparatively of agar heart–brain with violet crystal, phenethyl-alcohol and yolk emulsion that make the colonies to be visible justafter 72 h of incubation.
http://dx.doi.org/10.1016/j.jbiotec.2014.07.317
Detection of mechanism and anticancer activityof the new quinoline compounds MC20 andMC21*
Tugba Kul Köprülü 1,∗, Saban Tekin 2, SalihÖkten 3, Merve Cınar 4, Seda Duman 5, OsmanCakmak 4
1 Department of Biology, Gaziosmanpasa University,Faculty of Science & Art, Tokat, Turkey2 Department of Molecular Biology & Genetics,Gaziosmanpasa University, Faculty of Science & Art,Tokat, Turkey3 Primary Education, Division of Science Education,Faculty of Education, Kirikkale University, Kirikkale,Turkey4 Department of Chemistry, Faculty of Art andScience, Yildiz Technical University, Davutpasa,Istanbul, Turkey5 Departments of Chemistry, GaziosmanpasaUniversity, Faculty of Science & Art, Tokat, Turkey
E-mail address: tugbakul [email protected] (T.K. Köprülü).
Various quinoline compounds have received great attention dueto their potent biological activities. The aim of this study was toinvestigate the anticancer properties and mechanism of action ofthe novel quinoline derivatives, MC20 and MC21. Antiproliferativeand cytotoxic activities of MC20 and MC21 were tested on HeLa(human cervix carcinoma), HT29 (human colon carcinoma), andC6 (rat brain carcinoma) cell lines in vitro using BrdU cell prolifer-ation ELISA, sulphorhodamine B (SRB), and lactate dehydrogenase(LDH) assay. The mechanism of anticancer activity of MC20 and21 were determined using DNA laddering assay which indicatesapoptosis. Results indicated that the new quinoline derivatives,MC20 and MC21 were more antiproliferative and cytotoxic againsttumor cells than anticancer drug, 5-flourourasil (5-FU). MC20and MC21 caused DNA laddering in tumor cells in DNA ladderingassays, indicating that they prevent proliferation of tumor cells byinducing apoptosis. The results showed that the MC20 and MC21are potential anticancer drug candidate with high antiproliferativeactivity on tumor cells in vitro.
*This study is supported by a grant (TBAG 112T394) fromTUBITAK.
http://dx.doi.org/10.1016/j.jbiotec.2014.07.318
Diagnostic techniques performed before andafter the ablation of a tumored prostate andurinary bladder in a dog
Vulpe Vasile 1,∗, Pasca Aurelian Sorin 1, VulpeCristina Alice 2, Papuc Ionel 2
1 Department of Clinics, Veterinary Faculty,Agricultural Sciences and Veterinary Medicine, Iasi,Romania2 Department of Doctoral Schools, VeterinaryFaculty, Agricultural Sciences and VeterinaryMedicine, Cluj-Napoca, Romania
E-mail address: vasile [email protected] (V. Vasile).
In dogs, the prostate can suffer various processes, such as hyper-plasia or malignancy. The purpose of this paper is to underline theconnection between the clinical and imaging diagnostic techniquesand the surgical ones, while also preserving and ensuring the lifeof the patient. One Pekinese 14 year old dog was examined radio-
