The Next Generation of DNA Assembly and Cloning · * While supplies last. Offer valid in the US...

9
The Next Generation of DNA Assembly and Cloning NEW ENGLAND BIOLABS ® , NEB ® and NEBUILDER ® are registered trademarks of New England Biolabs, Inc. GIBSON ASSEMBLY ® is a registered trademark of Synthetic Genomics, Inc. * While supplies last. Offer valid in the US only. Limit one sample per customer. NEBuilder ® HiFi DNA Assembly The next generation of DNA assembly and cloning has arrived. With NEBuilder HiFi DNA Assembly, you’ll enjoy virtually error-free joining of DNA fragments. More efficient assembly is now possible, even with larger fragments, low inputs, or 5´- and 3´-end mismatches. Additionally, use NEBuilder HiFi to bridge two dsDNA fragments with a ssDNA oligo. Save time with less screening or re-sequencing, and benefit from no licensing fee requirements from NEB when choosing NEBuilder products. Request a free sample* at www.NEBuilderHiFi.com Total fragments assembled 0 20 40 Fragment size Vector size Total size (kb) 60 80 100 NEBuilder HiFi DNA Assembly Master Mix (NEB #E2621) Gibson Assembly Master Mix (NEB #E2611) Percent Correct 2 A 3 kb 5.4 kb 8.4 10/10 10/10 2 B 210 bp 7.7 kb 7.9 3/3 5/5 6 F 5 x 1 kb frags. 3.3 kb 8.3 11/12 1/10 3 E 450 bp + 250 bp 7 kb 7.7 12/12 5/8 2 D 3 kb 2.1 kb 5.5 10/10 7/10 2 C 210 bp 7.7 kb 7.9 5/5 1/4 NEBuilder HiFi DNA Master Mix offers improved fidelity over Gibson Assembly Master Mix Fidelity of assembled products was compared between NEBuilder HiFI DNA Assembly Master Mix (NEB #E2621) and Gibson Assembly Master Mix (NEB #E2611). Experiments were performed using the various fragment and vector sizes, following suggested protocols. Experiments B and C vary because sequences of fragments are different. Experiments D and F were performed with fragments containing 3´-end mismatches.

Transcript of The Next Generation of DNA Assembly and Cloning · * While supplies last. Offer valid in the US...

Page 1: The Next Generation of DNA Assembly and Cloning · * While supplies last. Offer valid in the US only. Limit one sample per customer. NEBuilder ® HiFi DNA Assembly The next generation

The Next Generation of

DNA Assembly and Cloning

NEW ENGLAND BIOLABS®, NEB® and NEBUILDER® are registered trademarks of New England Biolabs, Inc.

GIBSON ASSEMBLY® is a registered trademark of Synthetic Genomics, Inc.

* While supplies last. Offer valid in the US only. Limit one sample per customer.

NEBuilder® HiFi

DNA AssemblyThe next generation of DNA assembly andcloning has arrived. With NEBuilder HiFi DNAAssembly, you’ll enjoy virtually error-free joiningof DNA fragments. More efficient assemblyis now possible, even with larger fragments,low inputs, or 5´- and 3´-end mismatches.Additionally, use NEBuilder HiFi to bridge twodsDNA fragments with a ssDNA oligo. Save timewith less screening or re-sequencing, and benefitfrom no licensing fee requirements from NEBwhen choosing NEBuilder products.

Request a free sample*

at www.NEBuilderHiFi.com

Total fragmentsassembled

0

20

40

Fragment size

Vector size

Total size (kb)

60

80

100

NEBuilder HiFi DNA Assembly Master Mix (NEB #E2621)

Gibson Assembly Master Mix (NEB #E2611)

PercentCorrect

2

A

3 kb

5.4 kb

8.4

10/10 10/10

2

B

210 bp

7.7 kb

7.9

3/35/5

6

F

5 x 1 kb frags.

3.3 kb

8.3

11/12

1/10

3

E

450 bp + 250 bp

7 kb

7.7

12/12

5/8

2

D

3 kb

2.1 kb

5.5

10/10

7/10

2

C

210 bp

7.7 kb

7.9

5/5

1/4

NEBuilder HiFi DNA Master Mix offers improved fidelity

over Gibson Assembly Master Mix

Fidelity of assembled products was compared between NEBuilder HiFI DNA Assembly Master Mix

(NEB #E2621) and Gibson Assembly Master Mix (NEB #E2611). Experiments were performed

using the various fragment and vector sizes, following suggested protocols. Experiments B and C vary

because sequences of fragments are different. Experiments D and F were performed with fragments

containing 3´-end mismatches.

Page 2: The Next Generation of DNA Assembly and Cloning · * While supplies last. Offer valid in the US only. Limit one sample per customer. NEBuilder ® HiFi DNA Assembly The next generation

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Page 3: The Next Generation of DNA Assembly and Cloning · * While supplies last. Offer valid in the US only. Limit one sample per customer. NEBuilder ® HiFi DNA Assembly The next generation
Page 4: The Next Generation of DNA Assembly and Cloning · * While supplies last. Offer valid in the US only. Limit one sample per customer. NEBuilder ® HiFi DNA Assembly The next generation
Page 5: The Next Generation of DNA Assembly and Cloning · * While supplies last. Offer valid in the US only. Limit one sample per customer. NEBuilder ® HiFi DNA Assembly The next generation

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