The new UV/Vis generation – 886/887 Metrohm Ion Chromatography Dr. Katinka Ruth Product Specialist...

UV/Vis generation 886/887 1

The new UV/Vis generation – 886/887

Metrohm Ion Chromatography

Dr. Katinka Ruth

Product Specialist Competence Center ICMetrohm International Headquarters


Outline

The new UV/Vis generation – 886/887

• New UV/Vis detector• New heated reactor

• For post-column reactions• As column department

• Applications


887 Professional UV/Vis Detector

Hardware

• Diode array detector• UV lamp (deuterium) + Vis lamp (halogen)

Built in as in 844• 8 data channels + 1 reference channel• CH1/CH2 quotient channel omitted• 887 = standalone detector

Combination possible with 850, 881, 882

(≠ conductivity detector)• Connection to PC via USB• Analog out: up to 4 channels



Features

• Same functionality as 844• MagIC Net• T int, T out: New names for better understanding

Tint = integration time

Tout = measuring time• Measuring time = multiple of integration time• Records spectra at any point of the chromatogram


2 ppm nitrite, 4 ppm bromide, 50 ppm nitrate

in artificial sea water (28 g/L NaCl)

A Supp 10 - 250

5 mmol/L Na2CO3, 5 mmol/l NaHCO3

1.0 mL/min

MSM MCS (step after 13.5 min)

218 ± 5 nmSample volume 5 µLAccording to AW CH6-1027-012010

0.0

0.1

0.2

0.3

0.4

0.5

200 225 250 275 300 325 350 375

SpectrumRecord started 18.11.2009, 15:24

Inten

sity

Wavelength / nm

Retention time 9 min (nitrite) Retention time 19 min (bromide)

0.0

0.1

0.2

0.3

0.4

0.5

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0.7

200 225 250 275 300 325 350 375


Inten

sity

Wavelength / nm

0.0

0.1

0.2

0.3

0.4

0.5

0.6

0.7

200 225 250 275 300 325 350 375


Inten

sity

Wavelength / nm

Retention time 23 min (nitrate)

Feature: Spectrum at any time point


Evaluate absorbance maxima

0.0

0.1

0.2

0.3

0.4

0.5

0.6

0.7

200 225 250 275 300 325 350 375


Inten

sity

Wavelength / nm

Features

• Define start and end wave length• Automatic evaluation of absorbance maxima• Up to 10 automatic evaluations


Direct and indirect UV detection

Features

• Change polarity via MagIC Net


New assembly of lamps

Features

• Installation from the top• Easy mounting

6.2804.050 Deuterium lamp (UV)

6.2804.060 Halogen lamp (Vis)


887 Professional UV/Vis detector

Technical specifications

• Array with 512 diodes

• Channel λ 190 – 900 nm (increment 1)

• Bunching ± 0 – 100 nm (increment 1)

• Integration time 10 – 254 ms

• Measuring time 50 – 2000 ms

• Noise (expected) 0.1 – 0.2 mAU (similar to 844)

• Resolution 1.4 nm

• Metal free

• Leak sensor on tray

• PEEK detector cellOptical path



Possible combinations

• MagIC Net 2.2 = must• Stand alone UV detector• Controlled by MagIC Net Multi, Professional, and Compact





• 1 detector: MagIC Net Compact • 2 detectors: MagIC Net

Professional


881or

882

CompactIC

Connector

2.881.00X0 Compact IC pro

2.882.00X0 Compact IC plus

6.2061.120 System Connector ProfIC/Compact IC

2.887.0010 Professional UV/Vis Detector




• 1 detector: UV

850

ProfessionalIC


2.850.XXXX Professional IC

2.887.0010 Professional UV/Vis Detector σ σ

• 2 detectors: UV + conductivity

• 3 detectors: UV + 2 x conductivity

in series or parallel detection• MagIC Net Professional


New heated reactor – general info

• Heating up to 150°C, increment 0.1°C• Direct heat transfer, faster heating than 850, 881

<10 min (20°C to 40°C)• Temperature stability: better than 0.05°C• Temperature reproducibility: better than ± 0.2°C• Temperature accuracy: <1°C• MagIC Net 2.2 (Professional and Multi)• Combinations possible with 850, 887, 881, 882• Host for further 872 Extension modules• Robust design

• Two versions:

a) 2.886.0110 Professional Reactor

b) 2.886.0210 Professional Thermostat



For post-column-derivatisation (PCR)

• Intelligent reactor = iReactor

(with chip on reactor plate)• Temperatures up to 120°C• Space to have 4 reactors• Heated mixing-T

Accessories (included)

6.2744.330 Y-connector 3 x UNF 10/32

6.2845.100 Reactor plate complete to PCR Module

6.2845.200 Reactor complete to 6.2845.100

6.1015.100 Metrosep BP 1 Guard/2.0



Setup

• Base plate (iReactor)• + 1 reactor

(0.5 x 2000 mm PEEK-capillary)

dimensions as previous PCR-Box• + 2 reactor e.g. as preheating capillary

for eluent or post column reagent• Up to 4 reactors• Setup depends on application/desired

temperature


b) 2.886.0210 Professional Thermostat

Heated column department

• Compatible with intelligent columns• Column length up to 150 mm• Temperature up to 80°C

Accessories (included)

6.1836.020 Preheating capillary with core, 1.44 m

6.2845.600 Column holder complete to PCR Module


2.886.0210 Professional Thermostat

Setup

Up to 2 columns in the thermostat• e.g. column + pre-column• or 1 x 150 mm + 1 x 100 mm• 1 column is monitored

• Additional pre-heating capillary must be installed


Name reactor plate

Set to work

Last change

Comment

Reactor 1 mounted

Reactor 1 order number

Reactor 1 material

Reactor 1 length

Reactor 1 inner diameter

Reactor 1 comment

Intelligent Thermostat / Reactor

• Chip for reactors as at intelligent columns

• Monitoring / Traceability• 1 Chip per reactor plate

(iReactor)• Data field for up to 4 reactors

Reactor 2 mounted

Reactor 2 order number

Reactor 2 material

Reactor 2 length

Reactor 2 inner diameter

Reactor 1 comment

etc.


2.886.0110 Professional Reactor



886 ProfessionalReactor

872 IC Pump

881or

882

CompactIC

Connector2.881.00X0 Compact IC pro

2.882.00X0 Compact IC plus




2.872.0010 Extension Module IC Pump


• Transport of PCR-reagent:

High pressure iPump of 872 Extension module

• Use of BP 1 Guard






881or

882

CompactIC

Connector

2.881.00(2/3)0 Compact IC pro

2.882.00(2/3)0 Compact IC plus





881 peristaltic pump +

backpressure capillary• Regeneration MSM:

• 800 Dosino• Peristaltic pump of 872.0050

(for non-suppressed systems)

800




2.850.XXXX Professional IC





850 high pressure iPump

(alternative : peristaltic pump)

• Use back pressure column

together with iPump• Flexible detector setup

850

ProfessionalIC


886 Professional Reactor

σ σ






872 IC Module

872 IC Pump



2.872.0030 Extension Module IC Module

2.872.0010 Extension Module IC Pump

6.2061.100 Bottle holder (ProfIC)

6.2061.110 Tray with sensor



872 Extension Module IC Pump or

872 Sample Prep (peristaltic pump)

• Transport of eluent + injection:

872 Extension Module IC Module

• Bottle holder + tray





886 ProfessionalThermostat

872 IC Module



2.872.0030 Extension Module IC Module

6.2061.100 Bottle holder (ProfIC)

6.2061.110 Tray with sensor

• Simple stand-alone UV-system• 886 as column department• Bottle holder + tray


UV detection – suppressed or non-suppressed

+MSM +MCS +MSM-MCS –MSM-MCS

Noise 0.05 – 0.075 mAU Noise 0.05 – 0.075 mAU Noise 0.15 – 0.2 mAU500 ppb NO2

-, Br-, NO3- Mean (n = 8)

20 µL loop 218 ± 15 nm45°C Integration time 13 msA Supp 15 – 150/4.0 Measuring time 1 s0.7 mL/min 5 mM Na2CO3 + 0.3 mM NaOH


NO2

+MSM

+MCS+MSM-MCS

–MSM-MCS

Full width at half maximum 0.40 0.40 0.35Height 1.8 1.8 1.8Area 0.79 0.80 0.70Noise 0.09 0.09 0.18S/N 21 21 10Theoretical plates 1619 1362 2041

Br

+MSM

+MCS+MSM-MCS

–MSM-MCS


NO3

+MSM

+MCS+MSM-MCS

–MSM-MCS


Conclusion Area, height, noise, S/N = suppressed better

Theor. Plates = non-suppressed better



Recommendation• Chemical suppression (MSM)

Improvement for• Noise → S/N• System peak• Injection peak

• No MCS

Improvement for• Peak broadening• costs

+ MSM + MCS

+ MSM- MCS

- MSM- MCS



Applications

All existing applications published with 844 or Lambda 1010(reactor dimensions identical to previous PCR-Box)

• Direct UV/Vis detection• Indirect UV/Vis detection• Pre column derivatisation + UV/Vis detection• Post column derivatisation + UV/Vis detection


Applications

• Transition metals• Chromate• Bromate• Silicate• Cyanide, cyanogen chloride• Aluminium• ...

Potential new applications for PCR

• Amino acids• Ammonium next to high amounts of sodium• Au, Ag, Hg, Pt, Pd

Examples for Post column reactions (PCR)


Applications

887 Professional UV/Vis detector

500 ppb NO2-, NO3

- in artificial sea water (28 g/L NaCl)

A Supp 10 – 250/4.05 mmol/L Na2CO3, 5 mmol/l NaHCO30.7 mL/min5 µLMSM MCS218 nm ± 15 nmT int 13 ms, T out 1 s

AW CH6-1027-012010

NO2-

NO3-

Cl-


UV detection of nitrite and nitrate

Calibration 50 ppb – 20 ppmIon Relative

standard deviation

Correlation coefficient

Nitrite 1.163 0.999986

Nitrate 1.113924 0.999987


0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 min

0

100

200

300

400

mAU

Abs.1

Cu

Ni

Zn

Co

Pb

Mn

Cd

Applications

Transition metals

10ppm Cu, Ni, Zn, Co, Mn,20ppm Cd, 50ppm Pb

Metrosep C 5 – 150/4.615 mmol/L oxalic acid pH 3.5 (NH3)1.0 mL/min (eluent)0.5 mL/min (PCR)10 µL510 nm ± 11 nm

PCR-reagent0.15 mmol/L PAR0.4 mol/L NH3

80 mmol/L HNO3


Applications

Amino acids

USP 27 norm for amino acids• Requires 120°C• Possible with new PCR reactor

0.5 mM amino acids(20 compounds)Metrosep Amino Acids 1 – 100/4.0USP eluent570 nm

PCR-reagentNinhydrin, hydrindatindimethylsulfoxid,lithium acetate, acetic acid(N2 purged)


Applications

Bromate

• According to draft norms

ISO/DIS 11206 + EPA 326• PCR, tri-iodide method• UV absorbance at 352 nm

• Metrosep A Supp 16 – 100/4.0• 0.5 – 5 ppb Bromate

Bromate 4.8 min


Summary

UV/VIS generation – 886/887

• Professional UV/Vis detector

MagIC Net

8 channels (wavelength + bunching)

spectra at any time point

functionality as 844

• Professional Thermostat / Reactor

Faster heating + higher temperatures (direct heat transfer)

iReactor

2 MPa backpressure for smooth adding of reagent

• Available in summer 2010 (depending on MagIC Net 2.2)

The new UV/Vis generation – 886/887 Metrohm Ion Chromatography Dr. Katinka Ruth Product Specialist...

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