The Importance Of Isotype Controls In Flow Cytometry
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Transcript of The Importance Of Isotype Controls In Flow Cytometry
When To Use (And Not Use) Flow Cytometry Isotype Controls
W E P R O V I D E F L O W C Y T O M E T R Y T R A I N I N G T O A C A D E M I C S A N D
I N D U S T R Y P R O F E S S I O N A L S
When To Use (And Not Use) Flow Cytometry Isotype Controls
W E P R O V I D E F L O W C Y T O M E T R Y T R A I N I N G T O A C A D E M I C S A N D
I N D U S T R Y P R O F E S S I O N A L S
The world of science moves quickly these days. Things that were commonplace just a few
years ago can be made obsolete, leaving you scrambling to catch up
with the latest ideas.
When To Use (And Not Use) Flow Cytometry Isotype Controls
W E P R O V I D E F L O W C Y T O M E T R Y T R A I N I N G T O A C A D E M I C S A N D
I N D U S T R Y P R O F E S S I O N A L S
Case in point: flow cytometry isotype controls. Once considered
the gold standard as far as negative controls go, some
consider these to be antiquated technology.
When To Use (And Not Use) Flow Cytometry Isotype Controls
W E P R O V I D E F L O W C Y T O M E T R Y T R A I N I N G T O A C A D E M I C S A N D
I N D U S T R Y P R O F E S S I O N A L S
But are they right when they say to leave isotype controls out of just
about all your experiments?
When To Use (And Not Use) Flow Cytometry Isotype Controls
W E P R O V I D E F L O W C Y T O M E T R Y T R A I N I N G T O A C A D E M I C S A N D
I N D U S T R Y P R O F E S S I O N A L S
Find out more about these isotype matched controls (◄ read the full article here), and how you should (or shouldn’t!) use them in
your experiments from ExCyte, the leading authority on flow cytometry!
R E A D T H E F U L L A R T I C L E
When To Use (And Not Use) Flow Cytometry Isotype Controls
W E P R O V I D E F L O W C Y T O M E T R Y T R A I N I N G T O A C A D E M I C S A N D
I N D U S T R Y P R O F E S S I O N A L S
Of course, knowing when to use isotype controls is only one of the many
elements you need to know about clinical flow cytometry!
Another is knowing when to use the
proper flow cytometry analysis software!
R E A D T H E F U L L A R T I C L E
W E P R O V I D E F L O W C Y T O M E N T R Y T R A I N I N G T O A C A D E M I C S A N D
I N D U S T R Y P R O F E S S I O N A L S
A B O U T E X C Y T E C Y T O M E T R Y
Expert Cytometry, or ExCyte, was born out of the desire to make flow cytometry accessible to anyone interested in learning it. Flow Cytometry used to have an almost cultish existence, using battered notebooks to pass down knowledge. The problem was that this information generally never wnet beyond a particular institution. Learning was dependent on who you knew (or trial and error over the years), which slowed down progress. We formed ExCyte with the purpose of gathering people who are as passionate about learning successful flow cytometry as we are. Our goal is to make a lifetime of learning flow cytometry available to everyone. Our team has decades of experience teaching and managing flow labs and we collaborate closely with others like us to present a systematic, structured, and enjoyable program.
D I S C OV E R M O R E A B O U T E XC Y T E H E R E !
C O N N E C T W I T H E X C Y T E