The future of paper chromatography in the wine industry due to alternative methods

Carien Coetzee

SAWLA Workshop26 May 2017

Paper chromatography

Accuvin™Vinmetrica SC-

50

Enzymatic Spectrometric

Analysis

FTIRWinescan

HPLC

LOQ

Time for analyses

Hazards

Cost per sample

Instrument cost

Ease of use

Availability

Simpler methods Complex methods

Kit contains• Wide mouth jar• Capillary pipettes• Chromatography papers• Chromatography solvent

• Tartaric acid standard• Malic acid standard• Lactic acid standard

Paper

chromatography

Paper

chromatography

• Small sample volume• Limited training required• Readily available• Low cost• Kit only

• 24 hours• Hazardous chemicals• Not quantitative• Interpretation subjective

Malic acid

Lactic acid

Paper

chromatography

Kit contains• Sampler• Test strip• Colour chart

Accuvin™

Accuvin™

• Small sample volume• Limited training required• NOT Readily available ?• Low-medium cost

• Quantitative• 4 minutes• No Hazardous chemicals• Interpretation subjective• Kit only

• Vinmetrica SC-55 Manometer • Malic Reagent set• Biopressure Agent Reaction Vials• 1 ‘check’ vial • 10 ml serological pipette• 15 ml conical tube• Plastic bulb pipette• 100 mL polypropylene beaker• Plastic Scoop

• Distilled water• Microwave oven

Vinmetrica

SC-50

Vinmetrica

SC-50

• Small sample volume• Limited training required (more than previous)• Not Readily available ?• Low-medium cost

• Quantitative• 30-60 minutes• No Hazardous chemicals• Need to acquire instrumentation + kit

• Spectrophotometer • Cuvettes • Micropipettes • Enzyme test kit (includes buffer solution, NAD,

enzymes, standards)

Enzymatic

Spectrometric

Analysis

Enzymatic

Spectrometric

Analysis

• Small sample volume• Laboratory training required• Readily available• Low-medium cost per sample

• Quantitative• 30 minutes• No Hazardous chemicals• Need to acquire instrumentation + kit

Measure absorbance using a spectrophometer

Enzymatic

Spectrometric

Analysis

• Auto analyzers are convenient, but costly to purchase✓ Eliminates analyst variation✓ Less labour intensive ✓ Small volume needed✓ Capacity for increased sample numbers ✓ Decrease in cost per sample

• Skilled personnel • Periodic maintenance

• Foss Winescan / OenoFoss

FTIR

Winescan

FTIR

Winescan

• Winescan/OenoFoss is convenient, but costly to purchase✓ Eliminates analyst variation✓ Less labour intensive – very easy to use✓ Small volume needed✓ Capacity for increased sample numbers ✓ Decrease in cost per sample✓ Automization available

• Skilled personnel • Periodic maintenance• Constant checks due to matrix effects

• Small sample volume• Laboratory training required• Low-medium cost per sample

• Quantitative• 1 minute• No chemicals• Need to acquire instrumentation

• HPLC, column and assorted equipment • Sample vials and caps • Filtration membranes and syringes • Acetonitrile, phosphoric acid, and distilled water • Filtration unit for reagents

HPLC

HPLC

• Skilled personnel • Periodic maintenance

• Small sample volume• Laboratory training required• High cost per sample

• Quantitative• 40 minutes• Potentially hazardous chemicals• Need to acquire expensive

instrumentation

Paper chromatography

Accuvin™Vinmetrica SC-

50

Enzymatic Spectrometric

Analysis

FTIRWinescan/OenoFoss

HPLC

Qualitative Semi-Quantitative Quantitative Quantitative Quantitative Quantitative

LOQ n/a 0.03 g/L 0.1 g/L 0.05 g/L 0.001-0.05 g/L

Time for analyses

24 hours 4 min 30-60 min 30 min 1 min 40 min

Hazardschemicals noxious,

flamablenone irritant mildly corrosive none

chemicals flamable, corrosive, irritant

Cost per sample

R7 R40-R50 R40-R50R320 (after instrument)

R650 (after instrument)

Instrument cost

kit kit R2 000 R45 000 R1 200 000 R1 200 000

Ease of use * * ** *** *** ****

Availability **** * * *** *** ***

Paper

chromatographyAccuvin™

Vinmetrica

SC-50

Enzymatic

Spectrometric

Analysis

FTIR

WinescanHPLC

The future of paper chromatography in the wine industry due to alternative methods

✓ Easy to perform✓ Readily available✓ Low cost

• Useful for wineries: in-house checks• Can then confirm with accredited laboratory the completion of MLF• Not feasible in routine laboratories

Not Quantitative Does it need to be?

Questions?carien@vinlab.com

SAWLA Workshop26 May 2017