The Endomembrane System Text and image sources are included using the notes function of the.ppt...
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The Endomembrane System
Text and image sources are included using the notes function of the .ppt file
Cellular MembranesEndomembrane Structure % total membrane in
Hepatocyte% total in Pancreatic Exocrine Cell
Endoplasmic Reticulum (smooth)
16 <1
Endoplasmic Reticulum (rough)
35 60
Golgi 7 10
Plasma membrane 2 5
Lysosomes 0.4 N.D.
Peroxisomes 0.4 N.D.
Endosomes 0.4 N.D.
Mitochondria 39 21
Nuclear envelope (inner only)
0.2 0.7
Pulse-Label Autoradiography
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3 minutes 7 minutes
Pulse-Label Autoradiography
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37 minutes 117 minutes
The Endoplasmic Reticulum
DiOC6(3)
ER dynamics
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ER / Mitochondria
Video
“Smooth”ER
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Abnormal Pathology
sER from a chondrocyte of a patient with pseudoachondroplasia
Transition zones
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SER
RER
The rER
Supplying the NE
Movin’ on
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Note points of vesicle formation
Vesicular Traffic
donor membrane
recipient membrane
vesicle type
cell surface endosomes clathrin-coated
rER Golgi coatomerII-coated
Golgi Golgi coatomerI-coated
Golgi lysosomes clathrin-coated
Golgi cell surface coatomerI-coated
There are at least several (probably many more) distinct classes of vesicle coats/sorting mechanisms that function at different
places in the cell
coatomer-,smooth-coated vesicles
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Biochemical isolation of clathrin- coated vesicles
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Clathrin QuickTime™ and aTIFF (Uncompressed) decompressor
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Animation
Dynamin
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200 nm 100 nm
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shits wt
Breakin’ up is hard to do
Fusion
Just as there are specific mechanisms for sorting and vesicle formation, there are equally specific mechanisms for vesicle delivery
VSVGts::GFP transport
Vesicular stomatitis virus glycoprotein, temp-sensitive folding mutant
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Video
The Golgi
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Perinuclear Golgi
Biochemical stratification
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Post-Golgi transport tubules
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Video
Coatamer I Dynamics at the Golgi
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Fluorescence Recovery After Photobleaching (FRAP) of COPI::GFP
Lysosomes
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Exocytosis/Endocytosis
Peroxisomes
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