The Effects of Pollution on Bioluminescent Bacteria By: Tobi Simon.
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Transcript of The Effects of Pollution on Bioluminescent Bacteria By: Tobi Simon.
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The Effects of Pollution The Effects of Pollution on Bioluminescent on Bioluminescent
BacteriaBacteriaBy: Tobi SimonBy: Tobi Simon
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NeedNeed
Long-term exposureLong-term exposure Plants and Animals Plants and Animals
http://www.peternjenga.com/blogs/greenkenya/wp-content/uploads/2009/03/natural-and-manufactured-air-pollution.png
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Knowledge BaseKnowledge Base
http://nptel.iitm.ac.in/courses/Webcourse-contents/IIT-Delhi/Environmental%20Air%20Pollution/air%20pollution%20(Civil)/Module-1/images1/35.gif
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Knowledge BaseKnowledge Base
http://www.mcbs.unh.edu/faculty/Whistler/images/image3.jpg
Bioluminescent Bacteria
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Literature ReviewLiterature Review
Bolster, Carl H. "Comparison of E. coli and C. jejuni Transport Bolster, Carl H. "Comparison of E. coli and C. jejuni Transport in Saturated Porous Media." in Saturated Porous Media." Journal of Environmental QualityJournal of Environmental Quality (2006).(2006).
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Literature Review
Alvarez, David A. "Chemical and Toxicologic Assessment of Alvarez, David A. "Chemical and Toxicologic Assessment of Organic Contaminants in Surface Water Using Passive Organic Contaminants in Surface Water Using Passive Samples." Samples." Journal of Environmental QualityJournal of Environmental Quality (2008). (2008).
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Literature Review
Vanhaecke, Erwin. "Bioluminescence Assay for Measuring Vanhaecke, Erwin. "Bioluminescence Assay for Measuring the Number of Bacteria Adhering to the Hydrocarbon Phase the Number of Bacteria Adhering to the Hydrocarbon Phase in the BATH Test." in the BATH Test." Applied and Environmental MicrobiologyApplied and Environmental Microbiology (1988).(1988).
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Literature Review
Frischer, M. E. "Bioluminescent Bacteria as Indicators of Frischer, M. E. "Bioluminescent Bacteria as Indicators of Chemical Contamination of Coastal Waters." Chemical Contamination of Coastal Waters." Journal of Journal of Environmental QualityEnvironmental Quality (2005). (2005).
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PurposePurpose
The effects of pollution on the amount The effects of pollution on the amount of light emitted from bioluminescent of light emitted from bioluminescent bacteria.bacteria.
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HypothesisHypothesis
Alternate: Water with higher amounts Alternate: Water with higher amounts of hydrocarbons will cause a of hydrocarbons will cause a decrease in the amount of light decrease in the amount of light emitted from the bacteriaemitted from the bacteria
Null: Water with hydrocarbons in it will Null: Water with hydrocarbons in it will have no effect on the amount of light have no effect on the amount of light emitted from the bacteria. emitted from the bacteria.
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MethodologyMethodology
Water is collected from different locations and a sample of each is tested for the amount of hydrocarbons present.Bioluminescent bacteria are placed into different samples of water
After 24 hours the BLR will be calculated for each sample of water.
ControlNo hydrocarbon in water
Variable 1Lowest amount of hydrocarbon in water
Variable 2Medium amount of hydrocarbon in water
Variable 3Highest amount of hydrocarbon in water
Statistical Analysis: ANOVA followed by a Sheffe post hoc test
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Do AbilityDo Ability
OrganismsOrganisms ProceduresProcedures
http://www.abdn.ac.uk/staffpages/uploads/bch052/aglover-bac.jpg
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BudgetBudget
Vendor Order # Quantity Description PriceTotal Price
Sigma F7911-100EA 1 Millipore glass-fiber filters 86.7 86.7
Sigma A7002-250G 1 Agar 63 63
Sigma Peptone
Sigma S9148-1L 10 Seawater 20.7 207
Sigma Y1626-250G 1 Yeast Extract 115 115
Incubator
Spectrophotometer
Sigma P5481-500EA 1 Petri dishes 83.1 83.1
Carolina Science NP-15-5723 3 Vibrio fischeri 10.95 32.85
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BibliographyBibliography
American College of Gastroenterology. “Air Pollution May Increase Risk Of Appendicitis.” Science Daily 7 American College of Gastroenterology. “Air Pollution May Increase Risk Of Appendicitis.” Science Daily 7 October 2008. 23 April 2009October 2008. 23 April 2009
<http://www.sciencedaily.com- /releases/2008/10/081006102537.htm><http://www.sciencedaily.com- /releases/2008/10/081006102537.htm> Alvarez, David A. "Chemical and Toxicologic Assessment of Organic Contaminants in Surface Water Using Alvarez, David A. "Chemical and Toxicologic Assessment of Organic Contaminants in Surface Water Using
Passive Samples." Journal of Environmental Quality (2008).Passive Samples." Journal of Environmental Quality (2008). Bernstein, Leonard. Bernstein, Leonard. Environmental Science Ecology and Human Impact.Environmental Science Ecology and Human Impact. 2 2ndnd ed. Menlo Park, California: ed. Menlo Park, California:
Addison-Wesley Company, 1996.Addison-Wesley Company, 1996. "Bioluminescence." McGraw-Hill Encyclopedia of Science and Technology. 10th ed. 2007. "Bioluminescence." McGraw-Hill Encyclopedia of Science and Technology. 10th ed. 2007. Bolster, Carl H. "Comparison of E. coli and C. jejuni Transport in Saturated Porous Media." Journal of Bolster, Carl H. "Comparison of E. coli and C. jejuni Transport in Saturated Porous Media." Journal of
Environmental Quality (2006).Environmental Quality (2006). Bullock, Carole. “Air pollution may cause and speed up artery disease.” Bullock, Carole. “Air pollution may cause and speed up artery disease.” EurekAlert!EurekAlert! Frischer, M. E. "Bioluminescent Bacteria as Indicators of Chemical Contamination of Coastal Waters." Journal Frischer, M. E. "Bioluminescent Bacteria as Indicators of Chemical Contamination of Coastal Waters." Journal
of Environmental Quality (2005).of Environmental Quality (2005). Gulifoy, Christine. “Inhaling a heart attack: How air pollution can cause heart disease.” Gulifoy, Christine. “Inhaling a heart attack: How air pollution can cause heart disease.” EurekAlert!EurekAlert! International Union of Forest Research Organizations. “Damage To Forests From Climate Change Could Cost International Union of Forest Research Organizations. “Damage To Forests From Climate Change Could Cost
The Planet Its Major Keeper Of Greenhouse Gases, Study Warnes.” ScienceDaily. 21 April 2009. The Planet Its Major Keeper Of Greenhouse Gases, Study Warnes.” ScienceDaily. 21 April 2009. Safra, Jacob E. "Bioluminescence." The New Encyclopedia Britannica. 15th ed.Safra, Jacob E. "Bioluminescence." The New Encyclopedia Britannica. 15th ed. University of Arizona. “Fire Influences Global Warming More Than Previously Thought.” University of Arizona. “Fire Influences Global Warming More Than Previously Thought.” ScienceDailyScienceDaily 29 April 29 April
2009. 2009. http://www.sciencedaily.com/releases/2009/04/090423142332.htm.. University of Oxford. “Ice Sheet Behavior Much More Volatile And Dynamic Than Previously Thought, Tahiti University of Oxford. “Ice Sheet Behavior Much More Volatile And Dynamic Than Previously Thought, Tahiti
Corals Show.” Corals Show.” ScienceDailyScienceDaily 30 April 2009. 29 April 2009 30 April 2009. 29 April 2009 <http://www.sciencedaily.com/releases/2009/04/090423205104.htm><http://www.sciencedaily.com/releases/2009/04/090423205104.htm> Vanhaecke, Erwin. "Bioluminescence Assay for Measuring the Number of Bacteria Adhering to the Vanhaecke, Erwin. "Bioluminescence Assay for Measuring the Number of Bacteria Adhering to the
Hydrocarbon Phase in the BATH Test." Applied and Environmental Microbiology (1988).Hydrocarbon Phase in the BATH Test." Applied and Environmental Microbiology (1988).