The Effect of Ultra-High Molecular Weight Polyethylene Wear Debris on MG63 Osteosarcoma Cells in...
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![Page 1: The Effect of Ultra-High Molecular Weight Polyethylene Wear Debris on MG63 Osteosarcoma Cells in Vitro* by D. D. DEAN, Z. SCHWARTZ, Y. LIU, C. R. BLANCHARD,](https://reader035.fdocuments.in/reader035/viewer/2022062905/5a4d1ad77f8b9ab059973895/html5/thumbnails/1.jpg)
The Effect of Ultra-High Molecular Weight Polyethylene Wear Debris on MG63 Osteosarcoma Cells in Vitro*
by D. D. DEAN, Z. SCHWARTZ, Y. LIU, C. R. BLANCHARD, C. M. AGRAWAL, J. D. MABREY, V. L. SYLVIA, C. H. LOHMANN, and B. D. BOYAN
J Bone Joint Surg AmVolume 81(4):452-61
April 1, 1999
©1999 by The Journal of Bone and Joint Surgery, Inc.
![Page 2: The Effect of Ultra-High Molecular Weight Polyethylene Wear Debris on MG63 Osteosarcoma Cells in Vitro* by D. D. DEAN, Z. SCHWARTZ, Y. LIU, C. R. BLANCHARD,](https://reader035.fdocuments.in/reader035/viewer/2022062905/5a4d1ad77f8b9ab059973895/html5/thumbnails/2.jpg)
Figs. 1-A and 1-B: Scanning electron micrographs of ultra-high molecular weight polyethylene particles isolated from tissue obtained at the revision procedures (original magnification x 1000
and bar = 10.0 micrometers for Fig. 1-A; original magnification x ...
D. D. DEAN et al. J Bone Joint Surg Am 1999;81:452-61
©1999 by The Journal of Bone and Joint Surgery, Inc.
![Page 3: The Effect of Ultra-High Molecular Weight Polyethylene Wear Debris on MG63 Osteosarcoma Cells in Vitro* by D. D. DEAN, Z. SCHWARTZ, Y. LIU, C. R. BLANCHARD,](https://reader035.fdocuments.in/reader035/viewer/2022062905/5a4d1ad77f8b9ab059973895/html5/thumbnails/3.jpg)
Figs. 1-A and 1-B: Scanning electron micrographs of ultra-high molecular weight polyethylene particles isolated from tissue obtained at the revision procedures (original magnification x 1000
and bar = 10.0 micrometers for Fig. 1-A; original magnification x ...
D. D. DEAN et al. J Bone Joint Surg Am 1999;81:452-61
©1999 by The Journal of Bone and Joint Surgery, Inc.
![Page 4: The Effect of Ultra-High Molecular Weight Polyethylene Wear Debris on MG63 Osteosarcoma Cells in Vitro* by D. D. DEAN, Z. SCHWARTZ, Y. LIU, C. R. BLANCHARD,](https://reader035.fdocuments.in/reader035/viewer/2022062905/5a4d1ad77f8b9ab059973895/html5/thumbnails/4.jpg)
Fig. 2 Distribution of the sizes, expressed as equivalent circle diameters in micrometers, of the particles of wear debris isolated from tissue obtained at the revision procedures.
D. D. DEAN et al. J Bone Joint Surg Am 1999;81:452-61
©1999 by The Journal of Bone and Joint Surgery, Inc.
![Page 5: The Effect of Ultra-High Molecular Weight Polyethylene Wear Debris on MG63 Osteosarcoma Cells in Vitro* by D. D. DEAN, Z. SCHWARTZ, Y. LIU, C. R. BLANCHARD,](https://reader035.fdocuments.in/reader035/viewer/2022062905/5a4d1ad77f8b9ab059973895/html5/thumbnails/5.jpg)
Fig. 3 Effect of ultra-high molecular weight polyethylene particles on the number of MG63 cells.
D. D. DEAN et al. J Bone Joint Surg Am 1999;81:452-61
©1999 by The Journal of Bone and Joint Surgery, Inc.
![Page 6: The Effect of Ultra-High Molecular Weight Polyethylene Wear Debris on MG63 Osteosarcoma Cells in Vitro* by D. D. DEAN, Z. SCHWARTZ, Y. LIU, C. R. BLANCHARD,](https://reader035.fdocuments.in/reader035/viewer/2022062905/5a4d1ad77f8b9ab059973895/html5/thumbnails/6.jpg)
Fig. 4 Comparison of the effects of ultra-high molecular weight polyethylene particles from granulomatous tissue and the insoluble residue from control tissue on the number of cells (A)
and the alkaline-phosphatase-specific activity (B).
D. D. DEAN et al. J Bone Joint Surg Am 1999;81:452-61
©1999 by The Journal of Bone and Joint Surgery, Inc.
![Page 7: The Effect of Ultra-High Molecular Weight Polyethylene Wear Debris on MG63 Osteosarcoma Cells in Vitro* by D. D. DEAN, Z. SCHWARTZ, Y. LIU, C. R. BLANCHARD,](https://reader035.fdocuments.in/reader035/viewer/2022062905/5a4d1ad77f8b9ab059973895/html5/thumbnails/7.jpg)
Fig. 5 Effect of ultra-high molecular weight polyethylene particles on [3H]-thymidine incorporation by MG63 cells.
D. D. DEAN et al. J Bone Joint Surg Am 1999;81:452-61
©1999 by The Journal of Bone and Joint Surgery, Inc.
![Page 8: The Effect of Ultra-High Molecular Weight Polyethylene Wear Debris on MG63 Osteosarcoma Cells in Vitro* by D. D. DEAN, Z. SCHWARTZ, Y. LIU, C. R. BLANCHARD,](https://reader035.fdocuments.in/reader035/viewer/2022062905/5a4d1ad77f8b9ab059973895/html5/thumbnails/8.jpg)
Fig. 6 Effect of ultra-high molecular weight polyethylene particles on alkaline-phosphatase-specific activity of MG63 cells.
D. D. DEAN et al. J Bone Joint Surg Am 1999;81:452-61
©1999 by The Journal of Bone and Joint Surgery, Inc.
![Page 9: The Effect of Ultra-High Molecular Weight Polyethylene Wear Debris on MG63 Osteosarcoma Cells in Vitro* by D. D. DEAN, Z. SCHWARTZ, Y. LIU, C. R. BLANCHARD,](https://reader035.fdocuments.in/reader035/viewer/2022062905/5a4d1ad77f8b9ab059973895/html5/thumbnails/9.jpg)
Fig. 7 Effect of ultra-high molecular weight polyethylene particles on osteocalcin production by MG63 cells.
D. D. DEAN et al. J Bone Joint Surg Am 1999;81:452-61
©1999 by The Journal of Bone and Joint Surgery, Inc.
![Page 10: The Effect of Ultra-High Molecular Weight Polyethylene Wear Debris on MG63 Osteosarcoma Cells in Vitro* by D. D. DEAN, Z. SCHWARTZ, Y. LIU, C. R. BLANCHARD,](https://reader035.fdocuments.in/reader035/viewer/2022062905/5a4d1ad77f8b9ab059973895/html5/thumbnails/10.jpg)
Fig. 8 Effect of ultra-high molecular weight polyethylene particles on percent collagen production by MG63 cells.
D. D. DEAN et al. J Bone Joint Surg Am 1999;81:452-61
©1999 by The Journal of Bone and Joint Surgery, Inc.
![Page 11: The Effect of Ultra-High Molecular Weight Polyethylene Wear Debris on MG63 Osteosarcoma Cells in Vitro* by D. D. DEAN, Z. SCHWARTZ, Y. LIU, C. R. BLANCHARD,](https://reader035.fdocuments.in/reader035/viewer/2022062905/5a4d1ad77f8b9ab059973895/html5/thumbnails/11.jpg)
Fig. 9 Effect of ultra-high molecular weight polyethylene particles on [35S]-sulfate incorporation by MG63 cells.
D. D. DEAN et al. J Bone Joint Surg Am 1999;81:452-61
©1999 by The Journal of Bone and Joint Surgery, Inc.
![Page 12: The Effect of Ultra-High Molecular Weight Polyethylene Wear Debris on MG63 Osteosarcoma Cells in Vitro* by D. D. DEAN, Z. SCHWARTZ, Y. LIU, C. R. BLANCHARD,](https://reader035.fdocuments.in/reader035/viewer/2022062905/5a4d1ad77f8b9ab059973895/html5/thumbnails/12.jpg)
Fig. 10 Effect of ultra-high molecular weight polyethylene particles on prostaglandin-E2 production by MG63 cells.
D. D. DEAN et al. J Bone Joint Surg Am 1999;81:452-61
©1999 by The Journal of Bone and Joint Surgery, Inc.