The Effect of Electrical Stimulation on Adipose Stem Cells Cultured in Conductive Stereolithographic...
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The Effect of Electrical Stimulation on Adipose Stem Cells Cultured in
Conductive Stereolithographic Scaffold Structures
Suvi Haimi, PhDAdult Stem Cell Group, BioMediTech,
University of Tampere, Finland &
Department of Biomaterials Science and Technology, University of Twente, The Netherlands
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BioMediTech
• Tissue engineering and stem cell
technology
• Biomaterials
• Sensor and actuator technologies
• Measurement and imaging technologies
• Biotechnology
• Immunology
• Cancer and mitochondrial research
• Systems biology
• Bioinformatics
• Computational methods in biomedicine
BioMediTech is a joint institute of
University of Tampere & Tampere University of Technology
Over 250 scientists in world-class basic and translational research
Tampere, Finland
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Adult Stem Cell Group: world leader in stem cell based reconstructions of cranial bones
Example of Research Excellence
Combining stem cells with
biomaterial
Isolation of stem cells
GMP culture for 2-6 weeksTransplantation
back into same patient
Combination of biomaterials and stem cells differentiated from subcutaneous fat
Harvesting of adipose
tissue
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Department of Biomaterials Science and Technology
University of Twente
Enschede, The Netherlands
Stereolithography
• Polymer synthesis
• Polymer engineering
• State-of-the-art scaffold processing techniques
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Mechanical stress
CompressionStretch
Fluidflow
+-
Electrical stimulation (ES) in skeletal tissue engineering
Wound healing
Embryonic development
Action potential
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• Used in numerous biomedical applications
• Bioactive – can be easily incorporated with negatively charged biomolecules
Electrically conductive polypyrrole (PPy)
J Biomed Mater Res A, 2009
Langmuir, 2013
Tissue Eng Part A, 2013
Ann Biomed Eng, 2014
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Biocompatibility of PPy in vitro
Our team was the first to show
• Biocompatibility with ASCs
• Attachment and spreading can be enhanced by charging
J Biomed Mater Res A, 2009
Langmuir, 2013
Charged PPy (+)
Non-Charged PPy (0)
100 µm
100 µm
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Biocompatibility of PPy in vitro
My team was the first to show
• Biocompatibility with ASCs
• Attachment and spreading can be enhanced by charging
J Biomed Mater Res A, 2009
Langmuir, 2013
Charged PPy (+)
Uncharged PPy (0)
100 µm
100 µm
• Charging increased cell surface area of ASCs at 3h
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PPy promotes smooth muscle differentiation
Björninen et al. submitted
Calponin/DAPI MHC/DAPI SMA/DAPI
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Aim
Differentiate hASCs towards skeletal tissues applying ES via designed poly (trimethylene
carbonate) (PTMC) scaffolds coated with PPy
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Study design
Electroactive polypyrrole coating
3D electrical stimulation
Designed 3D scaffolds
Adipose stem cells
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Control ES 7d - rest 7d
ES 4h/d ES 8h/d0
1
2
3
4
5
6
7
α-SMA
Calponin
MHC
Re
lati
ve
ex
pre
ss
ion
at
14
d
/Electrical stimulation in 2D- Effects on smooth muscle differentiation
*
*
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control ES 4h/d ES 8h/d0.00
0.50
1.00
1.50
2.00
2.50
α-SMASM22αMHC
Re
lati
ve
ex
pre
ss
ion
at
14
d
Electrical stimulation in 3D-Effects on smooth muscle differentiation
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Electrical stimulation in 3D enhanced ASC osteogenic differentiation
*
*
14
d
ControlES
14 d
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Summary
• PPy-coated scaffolds are potential for skeletal tissue engineering
• ES is potential method to engineer muscle and bone tissue
• Screening of more effective ES parameters in 3D environment needed
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11.12.2008
Acknowledgements
TAMPERE UNIVERSITY OF TECHNOLOGY
Funding• Academy of Finland
• TEKES, Finnish Funding Agency for Technology and Innovation
• Pirkanmaa Hospital District Competitive research funding, EVO
• The City of Tampere
Adult Stem Cell GroupDepartment of Biomaterials Science and Technology
Key collaborators•
• Tampere University of Technology, Finland• VTT Technical Research Center,
Finland
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Kiitos / Thank You!