The Clinical Significance of Nuclear YAP Expression in Hepatocellular Carcinoma
description
Transcript of The Clinical Significance of Nuclear YAP Expression in Hepatocellular Carcinoma
![Page 1: The Clinical Significance of Nuclear YAP Expression in Hepatocellular Carcinoma](https://reader036.fdocuments.in/reader036/viewer/2022062323/56815d9d550346895dcbc369/html5/thumbnails/1.jpg)
1
The Clinical Significance of Nuclear YAP Expression
in Hepatocellular Carcinoma
徐 智南京医科大学附属南京第一医院暨
南京市第一医院 肿瘤内科
![Page 2: The Clinical Significance of Nuclear YAP Expression in Hepatocellular Carcinoma](https://reader036.fdocuments.in/reader036/viewer/2022062323/56815d9d550346895dcbc369/html5/thumbnails/2.jpg)
Worldwide incidence of HCC
Blum HE, Spangenberg HC. Arch Iran Med. 2007 Jul;10(3):361-71.
![Page 3: The Clinical Significance of Nuclear YAP Expression in Hepatocellular Carcinoma](https://reader036.fdocuments.in/reader036/viewer/2022062323/56815d9d550346895dcbc369/html5/thumbnails/3.jpg)
Identification of Yes-associated Protein (YAP)
Zender, L. et al. Cell 125, 1253-67
E
F
G
H
![Page 4: The Clinical Significance of Nuclear YAP Expression in Hepatocellular Carcinoma](https://reader036.fdocuments.in/reader036/viewer/2022062323/56815d9d550346895dcbc369/html5/thumbnails/4.jpg)
YAP improves cell proliferation
Time (Days)
* : p<0.05 。
![Page 5: The Clinical Significance of Nuclear YAP Expression in Hepatocellular Carcinoma](https://reader036.fdocuments.in/reader036/viewer/2022062323/56815d9d550346895dcbc369/html5/thumbnails/5.jpg)
YAP induces anchorage-independent growth
MIHA-YAP cells but not MIHA-Vec could form colonies in soft-agar.
![Page 6: The Clinical Significance of Nuclear YAP Expression in Hepatocellular Carcinoma](https://reader036.fdocuments.in/reader036/viewer/2022062323/56815d9d550346895dcbc369/html5/thumbnails/6.jpg)
MIHA-YAP cells gained advantages in cell migration and invasion.
YAP enhances cellular mobility and invasiveness
![Page 7: The Clinical Significance of Nuclear YAP Expression in Hepatocellular Carcinoma](https://reader036.fdocuments.in/reader036/viewer/2022062323/56815d9d550346895dcbc369/html5/thumbnails/7.jpg)
IHC results of YAP in HCC
53.7% HCC samples showed nuclear YAP positive.
![Page 8: The Clinical Significance of Nuclear YAP Expression in Hepatocellular Carcinoma](https://reader036.fdocuments.in/reader036/viewer/2022062323/56815d9d550346895dcbc369/html5/thumbnails/8.jpg)
Clinical association of nuclear YAP in HCC
![Page 9: The Clinical Significance of Nuclear YAP Expression in Hepatocellular Carcinoma](https://reader036.fdocuments.in/reader036/viewer/2022062323/56815d9d550346895dcbc369/html5/thumbnails/9.jpg)
Clinical association of nuclear YAP and HCC-specific postsurgical recurrence
Kaplan-Meier
Median RFT :Nuclear YAP negative: 25.8 months vs. Nuclear YAP positive : 12.9 months
![Page 10: The Clinical Significance of Nuclear YAP Expression in Hepatocellular Carcinoma](https://reader036.fdocuments.in/reader036/viewer/2022062323/56815d9d550346895dcbc369/html5/thumbnails/10.jpg)
Nuclear YAP is an independent prognostic marker for HCC recurrence
Cox Regression
![Page 11: The Clinical Significance of Nuclear YAP Expression in Hepatocellular Carcinoma](https://reader036.fdocuments.in/reader036/viewer/2022062323/56815d9d550346895dcbc369/html5/thumbnails/11.jpg)
Summary• YAP confers tumorigenic and metastatic potentials in
hepatocytes, as evidenced by enhanced aptitudes in cell viability, anchorage-independent growth, migration and invasion.
• YAP nuclear accumulation was found in the 53.7% of HCC, correlated with poorer tumor differentiation and higher serum alpha-fetoprotein (AFP) levels.
• Nuclear YAP is an independent predictor for HCC-specific recurrence-free survival.
• YAP plays an important role in HCC development and progression.
![Page 12: The Clinical Significance of Nuclear YAP Expression in Hepatocellular Carcinoma](https://reader036.fdocuments.in/reader036/viewer/2022062323/56815d9d550346895dcbc369/html5/thumbnails/12.jpg)
Acknowledgement
新加坡国立大学外科系和药学系 Asso. Prof. John M. Luk
香港大学外科系 Prof. Fan S. T. , Prof. Poon R. T. and
Surgical Tissue Bank
南京市第一医院肿瘤内科
![Page 13: The Clinical Significance of Nuclear YAP Expression in Hepatocellular Carcinoma](https://reader036.fdocuments.in/reader036/viewer/2022062323/56815d9d550346895dcbc369/html5/thumbnails/13.jpg)