The Beauty and Magic of Polarized Light
Transcript of The Beauty and Magic of Polarized Light
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The Beauty and Magic !of Polarized Light
• Polarized light is all around us• Demonstrations• How we use polarized light
Rudolf Oldenbourg Marine Biological Laboratory, Woods Hole, Massachusetts
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Polarization sensitive eyes
Cuttlefish
Monarch butterfly
but, alas, not the human eye
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Julien with camera and���polarizing filter
Julien de Groot
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reflections, water horizontal
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reflections, water vertical
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sky 0° vertical
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sky 0° horizontal
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sky 90° vertical polarization
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sky 90° horizontal
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Demos
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Stretched plastic film
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Stretched plastic film
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MBL photo contest 2009
• Winner, Chris Rieken, Megalops larval crab
Megalops larval crab, Chris Rieken
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MBL photo contest 2011
• Winner, Michael Shribak and Irina Arkhipova, Bdelloid rotifer (fresh water invertebrate)
Bdelloid rotifer, Michael Shribak and Irina Arkhipova
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Olympus photo contest 2005
• 4th prize, Rudolf Oldenbourg and James LaFountain, Crane fly spermatocyte
Spermatocyte, Rudolf Oldenbourg and James LaFountain
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Orientation dependent contrast��� in traditional polarizing microscope
P
A
optical section through aster
A
P
aster
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optical section through aster
Orientation dependent contrast��� in traditional polarizing microscope
C
P
A
A
P
C
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optical section through aster
Orientation dependent contrast��� in traditional polarizing microscope
C
P
A
A
P C
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Birefringence Imaging with the LC-PolScope
< 1 sec
< 0.5 sec
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Aster, retardance and slow axes color encoded
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Calcite film
calcite film by Dirk Volkmer, University of Ulm, Germany
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Meiosis I
cell preparation by James R. LaFountain, University at Buffalo
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Needle-shaped Crystals of Green Fluorescent Protein
crystal preparation by Naoki Noda, with Osamu Shimomura
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Fluorescence LC-PolScope
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Septin rearrangement in yeast bud neck
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Septin rearrangement in yeast bud neck
by Bradley DeMay and Amy Gladfelter
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Polarized light mural 4
YouTube video of polarized rainbow by Phil Plait, BadAstronomy.com
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Is the rainbow polarized?
Wikipedia
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Bibliography
• Optical Microscopy Primer, SFU, Michael Davidson���http://micro.magnet.fsu.edu/primer/lightandcolor/polarizedlighthome.html
• Hecht, E. (2002) Optics, San Francisco, CA: Pearson/Addison-Wesley
• Inoué, S. (2008) Collected Works of Shinya Inoué: microscopes, living cells, and dynamic molecules, Singapore: World Scientific.
• Website OpenPolScope.org