Testing for Lipids

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Te sting for Lipids (Fats) in a Food Sample Process  Add the food sample to two cubic centimetres of et hanol Shake well  Allow to settle in a test tube rack for two minutes Empty any clear liquid into a test tube containing two cubic centimetres of distilled water If the mixture remains clear there are no fats present in the sample However, if the mixture becomes cloudy there are fats present in the sample Explanation his test is called the emulsion test for lipids! "ipids are insoluble #will not dissolve$ in water and soluble #will dissolve$ in ethanol #an alcohol$!  After lipids have been dissolved in ethanol and then added to w ater, they w ill form tiny dispersed droplets i n the water ! his is called an emulsion! hese droplets are formed from a single layer of lipids arranged with their hydrophobic #water hating$ tails pointing inwards! hese droplets scatter light as it passes through the water so it appears white and cloudy!

description

A food test lab

Transcript of Testing for Lipids

7/16/2019 Testing for Lipids

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Testing for Lipids (Fats) in a Food Sample

Process

 Add the food sample to two cubic centimetres of ethanol

Shake well

 Allow to settle in a test tube rack for two minutes

Empty any clear liquid into a test tube containing two cubic centimetres ofdistilled water 

If the mixture remains clear there are no fats present in the sample

However, if the mixture becomes cloudy there are fats present in the sample

Explanation

his test is called the emulsion test for lipids!

"ipids are insoluble #will not dissolve$ in water and soluble #will dissolve$ inethanol #an alcohol$!

 After lipids have been dissolved in ethanol and then added to water, they willform tiny dispersed droplets in the water! his is called an emulsion!

hese droplets are formed from a single layer of lipids arranged with theirhydrophobic #water hating$ tails pointing inwards!

hese droplets scatter light as it passes through the water so it appears whiteand cloudy!