Telomeres in aging Universal STELA – a method to measure telomeres
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Telomeres in agingTelomeres in agingUniversal STELA – a method to measure Universal STELA – a method to measure
telomerestelomeres
Why do we age so differently?
Laila Bendix, MD
VELUX FONDEN
Funded by:
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Telomeres - structureTelomeres - structure
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Telomeres - function Telomeres - function Differentiating from DSB Shorten with cell aging
Harley et al 1990, Nature
End replication problem
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Telomeres relate to human Telomeres relate to human aging aging
Kimura et al 2008
Halaschek-Wiener et al 2008
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Do telomeres also shorten Do telomeres also shorten abruptlyabruptly
Signal free ends
Graakjaer et al, Aging Cell 2004
12 kb
8 kb
4 kb
1.6 kb
0.6 kb
0.1 kb
Very short telomeres
Baird et al, Nat Genet 2003
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Abrupt shortening - Abrupt shortening - mechanismsmechanisms
Passos et al 2007, NAR
Ferenac et al, 2005; J Gerontol A Biol Sci Med Sci
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OverskriftOverskrift
Tekst Punkt 1 Punkt 2
Underpunkt 1
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Universal STELA – assay Universal STELA – assay principlesprinciples
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Telomeres – Project part ITelomeres – Project part I
Short telomeres in organismal aging Does the number of short telomeres predict
mortality even better?
Number of short telomeres and DNA repair DNA repair functional studies Mitochondrial function Snp´s
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Telomeres and social classTelomeres and social class
What do we think telomere length is an age-related parameter that remains
aberrant after many years, and therefore is a suitable marker for social stress during life.
What do we know Individuals of lower socio-economic status have shorter
telomeres than their peers of a higher socio-economic status. Cherkas et al, 2006
No association between socio-economic status and telomere length. Adams et al, 2007
What do we have CAMB, MONICA and DARC Newer and better (?) methods
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Telomeres – Project part IITelomeres – Project part II
Establish a high throughput method to measure telomeres
Measure telomere lengths on samples collected in the CAMB study
… and later Examine other aging related molecular
biomarkers on same samples Short telomeres and social class