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Technology Leader in automatedFront-end Sample Handling

Spark Company ProfileSymbiosis XLC system with the new Reliance autosampler

Application examplesSymbiosis SPE-LC-MS and ion-suppression

May 2003

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Spark Holland

Company Profile

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Spark Holland B.V.

• Located in EMMEN• Premises 2150 m2

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EMMEN

AMSTERDAM

General information

• Founded: 1982• Activities:

♣ Development, marketing, sales and production of laboratory instruments.

• Market:♣ Automatic sampling and sample

preparation for LC and LC /MS.

• Revenues (2002): 15,2 mln Euro• Employees (2002): 79 f.t.e.• Ownership: Private• Quality system:

♣ ISO 9002 1993♣ ISO 9001 1996

1 9 9 7 1 9 9 8 1 9 9 9 2 0 0 0 2 0 0 1 2 0 0 2

Rev

enu

e

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Employees (December 2002)

F.T.EAssembly 33Product Development & Research 22Sales, Marketing and Service Group 16Quality Assurance 2Administration 5

Total employees: 79

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More than 20 OEM customers• Waters – cap-LC• Varian – Prostar• Amersham Pharmacia – Akta, Ettan• LC Packings/Dionex – Ultimate• Micromass – cap-LC• Beckman Coulter – System Gold• Polymer Labs – PSDA• Biochrom• ESA• Metrohm

Spark LC Autosamplers

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Prospekt –2 references (partial list)

Pharmaceutical companies:• Parke Davis/Pfizer• Glaxo Wellcome• Amgen• Biovitrum• Genentech• Novartis• Searle / Pharmacia Upjohn• Organon• Astra Zeneca• J&W Johnson• Schering• Schwarz Pharma• Eli LillyContract labs:• MDS Pharma Services• TNO Pharma• Xendo• Analytico• AAI• Alcontrol• Medeval

Major users• Pharmaceutical companies• Contract labs• Clinical labs• Environmental labs• Food & Beverage labs

• More than 600 Prospekt (1 & 2)systems installed around the world

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What’s in the sample?

The daily routine “bottleneck”:

How do I get my answer fast ?

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SamplePrep

The broken chain

Information flowInformation flow

SampleList

MSsoftware

Report Result

Physical sample flowPhysical sample flow

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Prospekt-2: the missing link

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SampleList

MSsoftware

Report Result

SparkLink™Software

1. Insert raw sample1. Insert raw sample

2. Prospekt2. Prospekt processingprocessing

3. Collect MS results3. Collect MS results

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Symbiosis System

Reliance autosampler – LC pumps – Prospekt-2 system

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Protect your investment:Symbiosis – XLC system

• Universal sample prep system: XLC

• Different techniques inside the system, no hardware changes

• Use existing sample prep with LC function

• Develop new methods with the XLC system

LC

Off-line SPE

Liq/Liq

Pro Precip.

On-line SPE

Fast SPE

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Symbiosis Pre-Fixed Tubing Setup

Features of XLC:

No hardware changes needed

Fast switching between:

1. Direct injection (LC mode)

2. On-line SPE (XLC mode)

3. Fast SPE (XLC mode)

4. SPE-LC focusing (XLC mode)

5. SPE method development

Universal sample prep system

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Cooling stacker and Reliance

Deep – shallow well, 96 – 384 wells, 48 vial adaptors / plate

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Cooled stacker - Reliance autosampler

True 4° C coolingin the sample well duringsample storage andsample processing!!

Supportcooling

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Cooling of 96-well Titerplate

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Reliance Injection System

Features:

• Injection cycle, including washes,less than 60 seconds

• PEEK – fused silica – stainless steelsample tubing / needle

• Low carry-over spec: < 0.01 %(measured below 0.005 %)

• Multiple wash solvents

• Large wash volumes (700 µL to 10 mL)

• Active inside – outside wash of sampletubing / needle

• Variable puncturing depths

• Injection volumes:1 µL and higher with steps of 0.1 µL

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Stacker: “cooling” hotel

Features:• Thermo stating from 4 °C - 20 °C

• Circulating forced air

• Accurate thermo stating of the sample well

• No condensation effects, reliable mechanics

• ∆ T measured: 29 °C (Peltier cooling)

Capacity:• 96 or 384 well plates

• 12 deep well plates or

• 24 shallow well plates or

• 6 deep well and 12 shallow well plates combined

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Symbiosis - XLC beta-test site:

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Symbiosis – XLC system: The Integrated Solution

HelpdeskApplication

supportInstallation& training Userforum

Single package

Cartridges

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Symbiosis – XLC System

Pharmaceutical Lab on 4Pharmaceutical Lab on 4feet of bench spacefeet of bench space

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Symbiosis – XLC System

Sample Preparation at the power of 2 !