Team Chem Biological Water Filtration. Purification Process Add cells to possibly contaminated water...
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Transcript of Team Chem Biological Water Filtration. Purification Process Add cells to possibly contaminated water...
![Page 1: Team Chem Biological Water Filtration. Purification Process Add cells to possibly contaminated water Cells take in arsenic and store it Cells provide.](https://reader035.fdocuments.in/reader035/viewer/2022070414/5697c0251a28abf838cd5377/html5/thumbnails/1.jpg)
Team ChemBiological Water Filtration
![Page 2: Team Chem Biological Water Filtration. Purification Process Add cells to possibly contaminated water Cells take in arsenic and store it Cells provide.](https://reader035.fdocuments.in/reader035/viewer/2022070414/5697c0251a28abf838cd5377/html5/thumbnails/2.jpg)
Purification Process
• Add cells to possibly contaminated water
• Cells take in arsenic and store it
• Cells provide an output (color) signaling the water is clean or needs further processing
• Separate of cells from water
• Possibility for further processing
![Page 3: Team Chem Biological Water Filtration. Purification Process Add cells to possibly contaminated water Cells take in arsenic and store it Cells provide.](https://reader035.fdocuments.in/reader035/viewer/2022070414/5697c0251a28abf838cd5377/html5/thumbnails/3.jpg)
Devices
• 2 Arsenic Sensors– Sensitive: activated by any arsenic – Non-sensitive: activated by maximum
tolerable arsenic level
• 2 Color Generators
• Arsenic Transport and Storage Device
![Page 4: Team Chem Biological Water Filtration. Purification Process Add cells to possibly contaminated water Cells take in arsenic and store it Cells provide.](https://reader035.fdocuments.in/reader035/viewer/2022070414/5697c0251a28abf838cd5377/html5/thumbnails/4.jpg)
Arsenic Transport and Storage
Remove the gene that encodes this transport protein
Tripathi, R.D., Srivastava, S., Mishra, S., Singh, N., Tuli, R., Gupta, D.K. and Maathuis, F. Arsenic Hazards: Strategies for Tolerance and Remediation by Plants. Trends in Biotechnology. 2007, 25.4, 158-165.
Arsenic Measurement
![Page 5: Team Chem Biological Water Filtration. Purification Process Add cells to possibly contaminated water Cells take in arsenic and store it Cells provide.](https://reader035.fdocuments.in/reader035/viewer/2022070414/5697c0251a28abf838cd5377/html5/thumbnails/5.jpg)
Device Level Diagram
Color Generator 1
(constitutively active)
Sensitive Arsenic Sensor
Non-Sensitive Arsenic Sensor Color Generator 2
Arsenic Transport and Storage Device
(constitutively active)
Where is Concentration
Read?
Outside Vacuole
Inside Vacuole or
with a Timer
![Page 6: Team Chem Biological Water Filtration. Purification Process Add cells to possibly contaminated water Cells take in arsenic and store it Cells provide.](https://reader035.fdocuments.in/reader035/viewer/2022070414/5697c0251a28abf838cd5377/html5/thumbnails/6.jpg)
Simulation: Pure Water
Green Color Generator
(constitutively active)
Sensitive Arsenic Sensor
Non-Sensitive Arsenic Sensor Red Color Generator
Arsenic Transport and Storage Device
(constitutively active)
Result: Cells Stay Green → Separation
![Page 7: Team Chem Biological Water Filtration. Purification Process Add cells to possibly contaminated water Cells take in arsenic and store it Cells provide.](https://reader035.fdocuments.in/reader035/viewer/2022070414/5697c0251a28abf838cd5377/html5/thumbnails/7.jpg)
Simulation: Maximum Arsenic
Result: Cells Turn Red → Separate and Process Again
Green Color Generator
(constitutively active)
Sensitive Arsenic Sensor
Non-Sensitive Arsenic Sensor Red Color Generator
Arsenic Transport and Storage Device
(constitutively active)
As As MAXIMUM
ARSENIC
![Page 8: Team Chem Biological Water Filtration. Purification Process Add cells to possibly contaminated water Cells take in arsenic and store it Cells provide.](https://reader035.fdocuments.in/reader035/viewer/2022070414/5697c0251a28abf838cd5377/html5/thumbnails/8.jpg)
Timing Diagram: Maximum Arsenic
0
1
0
1
0
1
0
1
0
1
Green Color Generator
Sensitive [As] Sensor
Transport and Storage
Non- Sensitive
[As] Sensor
Red Color Generator
t=0No Arsenic
Contaminated Water Added
Midlevel [As] Maximum [As]
![Page 9: Team Chem Biological Water Filtration. Purification Process Add cells to possibly contaminated water Cells take in arsenic and store it Cells provide.](https://reader035.fdocuments.in/reader035/viewer/2022070414/5697c0251a28abf838cd5377/html5/thumbnails/9.jpg)
Timing Diagram: Midlevel Arsenic
0
1
0
1
0
1
0
1
0
1
Green Color Generator
Sensitive [As] Sensor
Transport and Storage
Non- Sensitive
[As] Sensor
Red Color Generator
t=0No Arsenic
Contaminated Water Added
Midlevel [As] Maximum [As]
![Page 10: Team Chem Biological Water Filtration. Purification Process Add cells to possibly contaminated water Cells take in arsenic and store it Cells provide.](https://reader035.fdocuments.in/reader035/viewer/2022070414/5697c0251a28abf838cd5377/html5/thumbnails/10.jpg)
Biological PartsBackground System
ARR2ARR1
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Biological PartsControl System
YCF1YCF1 GSH1GSH1
RCGD:BBa_K274100
RCGD:BBa_K274100
GCGD:BBa_K274004
GCGD:BBa_K274004
X-RepressorX-Repressor
[As]
P(x)
P(y)
P(z)Y-RepressorY-Repressor
[As]max
![Page 12: Team Chem Biological Water Filtration. Purification Process Add cells to possibly contaminated water Cells take in arsenic and store it Cells provide.](https://reader035.fdocuments.in/reader035/viewer/2022070414/5697c0251a28abf838cd5377/html5/thumbnails/12.jpg)
Cellular Chassis
[Source:http://www.botany.hawaii.edu/nlc_biology/1406/lab/r2/slide6.jpg]
[Source: http://www.ppws.vt.edu/scott/weed_id/eldde.htm]
![Page 13: Team Chem Biological Water Filtration. Purification Process Add cells to possibly contaminated water Cells take in arsenic and store it Cells provide.](https://reader035.fdocuments.in/reader035/viewer/2022070414/5697c0251a28abf838cd5377/html5/thumbnails/13.jpg)
Testing/Debugging• Cell Death
– Effectiveness of ARR1, ARR1, YCF1, GSH1
• Need to determine Vacuole limits
• Testing Color Generators
• Promoter Design
[Source: http://www.sciencephoto.com/images/]
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Global Impacts
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Impacts
If this process succeeds:
• Solves one of the most problematic issues of the developing world, saving millions of lives. – 70 million people are affected in
Bangladesh alone - arsenic in ground water is the cause of 23% of all the deaths there!
![Page 16: Team Chem Biological Water Filtration. Purification Process Add cells to possibly contaminated water Cells take in arsenic and store it Cells provide.](https://reader035.fdocuments.in/reader035/viewer/2022070414/5697c0251a28abf838cd5377/html5/thumbnails/16.jpg)
Why Biological Water Filtration by Team Chemistry?
• No energy requirement (operates independent of electricity)– Accessibility to effective water filtration
technology even in rural parts of the developing world
• Very low cost of sustaining system
• The end product is not a waste– Possibility of selling to pharmaceutical companies
and turning a profit
![Page 17: Team Chem Biological Water Filtration. Purification Process Add cells to possibly contaminated water Cells take in arsenic and store it Cells provide.](https://reader035.fdocuments.in/reader035/viewer/2022070414/5697c0251a28abf838cd5377/html5/thumbnails/17.jpg)
Open Issues
• Elodea is the current choice of organism– Common water weed: robust, packaged for
surviving in water• The cost of entire project is unknown.• Legal issues attached to introducing
genetically modified organisms into the environment?
• Length of effectiveness is unknown – (How long until the organism reaches max arsenic
uptake capacity?)• The process of genetically modifying plants is
very slow…
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Go/ No Go?
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Citations
• Saccharomyces Genome Database www.yeastgenome.org
• Standard Registry of Biological Parts www.partregistry.org