sigma-aldrich.com

Sigma and Sigma-Aldrich are registered trademarks belonging to Sigma-Aldrich Co. and its affiliate Sigma-Aldrich Biotechnology, L.P.

CompoZr is a trademark of Sigma-Aldrich Co. and its affiliate Sigma-Aldrich Biotechnology, L.P.

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Targeted genome editing

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GeneSieve™, StellARray™, and Global Pattern Recognition™ are trademarks of Bar Harbor BioTechnology, Inc.

The StellARray™Gene Expression SystemWhen using prede�ned normalizers, one often ignores small

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GE Healthcare

Whatman is now partof GE Healthcare.

The considerable expertise of Whatman in the fields of sample preparation and filtration technologies has earnedthem an outstanding reputation world wide. Whatman has key enabling technologies used in the understanding ofgene and protein functions. Combining the skills of the two companies will enable GE Healthcare to create strongadded value for customers giving unparalleled support to a wide range of research and lab applications.

For more information visit www.gelifesciences.com/whatman

Creating a centre of excellence in separation technology.

QIAGEN Sample & Assay Technologies

ProteinSample & AssayTechnologies

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MolecularDiagnostics*Sample & AssayTechnologies

Explore sample and assay technologies today at www.qiagen.com !

* artus PCR assays are not cleared for clinical use in USA and Canada.Corp

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The incorporation of newly synthetisized proteins into the plasma membrane can be tracked in

the Leica AM TIRF MC microscope – living up to new insights in life.

Prof. Dr. Ralf Jacob, University Marburg, Department of Clinical Cytobiology and Cytopathology,

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Living up to Life

To find your local sales office, visitwww.bio-rad.com/contact/In the U.S., call toll free at 1-800-4BIORAD (1-800-424-6723)

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Opening New Doors in Primary Cell ResearchThe Gene Pulser MXcell™ electroporation system delivers molecules efficiently into mammalian cells — especially

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■ Primary cell electroporation protocols online at www.bio-rad.com/genetransferprotocols/

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Vary conditions rapidly to improve your results, following our guidelines.

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Gene Transfer | Electroporation

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NEW

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Introducing Crimson Taq™

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For more information and international distribution network, please visit www.neb.comNew England Biolabs Inc. 240 County Road, Ipswich, MA 01938 USA 1-800-NEB-LABS Tel. (978) 927-5054 Fax (978) 921-1350 info@neb.com

Canada Tel. (800) 387-1095 info@ca.neb.com • China Tel. 010-82378266 beijing@neb-china.com • Germany Tel. 0800/246 5227 info@de.neb.com

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Looking for the right solution for your high yield PCR? Choose from an expanded selection of

recombinant Taq DNA Polymerase based products from New England Biolabs. And for even greater

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Performance – Convenience – Results.

Taq DNA Polymerase from New England BiolabsHIGH YIELD, ROBUST AND RELIABLE PCR REACTIONS IN CONVENIENT FORMATS

Choose Crimson Taq™ DNA Polymerase for robust reactions in a convenient

format. Amplicon sizes indicated below gel. Marker M is the 1 kb DNA Ladder

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0.6 1.2 1.5 2.8 4.0 5.0 M

kb

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Amplification of human genomic DNA with Crimson Taq™

n NEW Crimson Taq™ DNA Polymerase r . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . M0324S/L

n LongAmp™ Taq 2X Master Mix r . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . M0287S/L

n LongAmp™ Taq DNA Polymerase r . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . M0323S/L

n LongAmp™ Taq PCR Kit r . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . E5200S

n Quick-Load ™ Taq 2X Master Mix r . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . M0271S/L

n Taq 2X Master Mix r . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . M0270S/L

n Taq 5X Master Mix r . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . M0285S/L

n Taq PCR Kits r . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . E5000S/E5100S

n Taq with Standard Taq Buffer r . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . M0273S/L/X

n Taq with Standard Taq (Mg-free) Buffer r . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . M0320S/L

n Taq with ThermoPol Buffer r . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . M0267S/L/X

n Taq with ThermoPol II (Mg-free) Buffer r . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . M0321S/L

= Recombinantr

■ Obtain sequencing read lengths of 400 to 500 bases.

■ Generate more than 1 million sequencing reads per

10-hour instrument run.

■ Improve performance by using GS FLX Titanium reagents —

without instrument upgrades.

■ Perform more applications with longer sequencing reads.

Learn more at www.genome-sequencing.com

Length Matters

Introducing the GS FLX Titanium Reagents

www.roche-applied-science.com

Genome Sequencer FLX System

For life science research only. Not for use in diagnostic procedures.

454, 454 LIFE SCIENCES, and 454 SEQUENCING are trademarks of 454 Life Sciences

Corporation, Branford, CT, USA, a Roche company. GS FLX TITANIUM is a trademark of Roche.

Other brands or product names are trademarks of their respective holders.

© 2008 Roche Diagnostics GmbH. All rights reserved.

Roche Diagnostics GmbH

Roche Applied Science

68298 Mannheim, Germany

DNA Sequencing Flowgram: Each bar within the flow-

gram represents a discrete nucleotide (A, T, C, or G) and

the height of the bar corresponds to the number of

nucleotides detected. The flowgram above represents a

458-base-pair sequencing read from E. coli K-12.

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Your local distributor www.eppendorf.com/worldwide · Application Support E-mail: support@eppendorf.com

Eppendorf AG · Hamburg · Germany · Tel: +49 40 538 01-0 · Eppendorf North America, Inc. · USA · Tel: +1 800 645 3050

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Each single of your valuable samples deserves

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Sample loss can be time consuming and expensive.

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Eppendorf consumables are designed to cover all of the

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Make the best of it!Top quality for your sample.

Eppendorf Consumables – it’s your sample

Experience the power of dynamic, real-time, label-free cellularanalysis with the xCELLigence System from Roche Applied Science.Acquire data that end-point analysis could never realize, throughoutyour entire experiment. Work label-free to ensure physiologicallyrelevant data, and choose from flexible throughput options to meetyour needs: 24, 96, or 576 (6 x 96) wells simultaneously.

■ Capture data throughout the entire time course of your

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■ Obtain physiologically relevant data and eliminate the need

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The xCELLigence System, providing Greater Insight for

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For more information, visit www.xcelligence.roche.com or

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Figure 1: Real-time monitoring of cytotoxicity

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Greater Insight, True Understanding

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xCELLigence Real-TimeCell Analyzer System

Control

12.5 µM Etoposide

25 µM Etoposide

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Roche Diagnostics GmbH

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68298 Mannheim, GermanyXCELLIGENCE is a trademark of Roche. © 2008 Roche Diagnostics GmbH. All rights reserved.

RTCA SP Instrument

New PureYield™ Plasmid Miniprep yields transfectable

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centrifugation or vacuum protocols. Like all Promega

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realizing valuable time savings for your lab?

Prove it to yourself. To learn more and

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visit: www.promega.com/plasmid

New PureYield™ Plasmid Miniprep yields transfectable

plasmid DNA in as little as ten minutes using either

New System Answers Needfor Speed, Purity and Yield

©2008 Promega Corporation, 16405-AD-GN

PureYield™

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Done in10 min.

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TMT

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Work in tandem for absolute total protein quantitation.

Accurately identify and quantify thousands of proteins in a given

sample – in a single experiment. Use the Thermo Scientific

Tandem Mass Tag (TMT®) Kits with your mass spectrometry

instrument for high-throughput protein biomarker discovery.

• ID and quantitate multiple samples from cells, tissues

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• Quantitatively analyze proteins for which no antibodies

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To learn more, visit www.thermo.com/TMT or call 815-968-0747

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Moving science forward

A total solution for quantitative protein

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TMT Protein Labeling Chemistry complements

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instruments and software.

Higher Speci�city: Nomore non-speci�c ampli�cation

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amount of template.

Wide Range of Detection: accurate quanti�cation

over 8 log of magnitude.

Simple Protocol: Ready to use premix type. Short reaction

time due to the antibody mediated hot start technology.

Fast Reaction Times: Shorter reaction times due to opti-

mized bu�er. Applicable for use with Fast PCR instruments.

Versatility: Use on any qPCR instrument.

The Perfect Real Time series introduces a new addition, the

SYBR® Premix Ex TaqTM 2 reagent for Real Time PCR. This

reagent has improved reaction speci�city and performance

and is compatible with a variety of di�erent Real Time

machines. The SYBR® Premix Ex TaqTM 2 e�ectively limits

primer dimerization and non-speci�c ampli�cation and

accurately measures a wide range of concentrations from

very small amounts of template. SYBR® Premix Ex TaqTM 2 ,

the latest PCR premix from Takara, is unsurpassed for high-

speed, high-speci�city application results.

Ampli�cation of cDNA from theMouse geneYWHAZ

using Takara’s Thermal Cycler Dice® Real Time System*.

6.4 pg-100 ng of total RNA frommouse liver was used to tran-

scribe cDNA for ampli�cation. (*Not available in Europe)

SYBR® Premix Ex Taq™ II

High Specificity with any Template Amount

PCR Products

For more information

www.takara-bio.com

Japan:

Takara Bio Inc.+81 77 543 7247www.takara-bio.com

USA:

Takara Bio USAA division of Clontech Laboratories, Inc

888-251-6618www.takarabiousa.com

Europe:

Takara Bio Europe S.A.S.+33 1 3904 6880www.takara-bio.eu

China:

Takara Biotechnology(Dalian) Co., Ltd.+86 411 8764 1681www.takara.com.cn

Korea:

Takara KoreaBiomedical Inc.+82 2 2081 2525www.takara.co.kr

TaKaRa Ex Taq™ is a trademark of Takara Bio Inc. SYBR® is a registered trademark of Molecular Probes,

Inc. Purchase of this product includes an immunity from suit under patents speci�ed in the product

insert to use only the amount purchased for the purchaser’s own internal research. No other patent

rights are conveyed expressly, by implication, or by estoppel. Further information on purchasing

licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln

Centre Drive, Foster City, California 94404, USA. Takara Bio’s Hot-Start PCR-Related products are

licensed under U.S. Patent 5,338,671 and 5,587,287 and corresponding patents in other countries.

MyCube by Antarus

Biotech is a powerful

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Huntsville, Alabama

genomic research • economic development • educational outreach • hudsonalpha.org

Antarus is one of the companies located with HudsonAlpha on the150-acre CRP Biotech Campus. For information about the campusand site availability, bizdev@hudsonalpha.org.

(actual size)

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Personal

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1µl samples • increased throughput

The power of small

NanoDrop 8000Spectrophotometer

x8

Revolutionary technology. 8 readings in under 30 seconds.The Thermo Scientific NanoDrop™ 8000 Spectrophotometer ispowerful — eight 1µl samples at once — now with the newSample Position Illuminator.

Full spectrum UV/Vis analysis of 1µl samples for quantitation,purity assessments and more: nucleic acids, microarrays,proteins and general spectrophotometry.

Measurement is quick and easy — pipette up to eight samplesand measure. Each sample is read using two path lengths toachieve an extensive dynamic range (e.g., 2-3700 ng/µl dsDNA),virtually eliminating the need for dilutions. Then just a quickwipe clean and you’re ready for your next samples. What couldbe easier — or more powerful?

And for the power of small in single-sample absorbance orfluorescent measurements, check out the NanoDrop 1000Spectrophotometer or the NanoDrop 3300 Fluorospectrometer(ultra low fluorescent detection limit of sample mass — e.g.,2 pg dsDNA).

Ready to experience the power of small x8? Test a NanoDrop8000 Spectrophotometer in your own lab. Contact us tolearn about our FREE one-week evaluation and limited-timetrade up offer.*

www.nanodrop.com | 302.479.7707

*Available in US and Canada only.

©2008Therm

oFisherScientificInc.

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Part of Thermo Fisher Scientific

Same people. Same support. New possibilities.

©2

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If you work with proteins, you’ve relied on Pierce reagents. For gene silencing,

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screening, you’ve trusted Cellomics and BioImage.

Now these top names in life science are sold under the Thermo Scientific brand.

You’ll still find the same technical support and application expertise that you’ve

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So yes, there’s a new brand name – Thermo Scientific. But you can keep your

trusted relationships and high level of service as we unite to help you

pursue new possibilities.

Moving science forward

Learn more at www.thermo.com/LSR.

www.sciencemag.org/products SCIENCE VOL 321 12 SEPTEMBER 2008 1511

LIFE SCIENCE TECHNOLOGIESAAAS/Science Business Office

Fluorescence StereomicroscopesThe Leica M205 FA and M165 FC stereomicroscopes are fordemanding fluorescence applications in developmental, molecular,and cellular biology. The microscopes unite top performancezoom optics, resolution, and contrast to produce fluorescenceimages with outstanding richness of detail. The instrumentsfeature patent-pending FusionOptics technology, which takesadvantage of a neurological phenomenon: The left beam pathproduces great depth of field, while the right beam path providesa high-resolution image. The human brain then combines the bestinformation from both channels, using it to compose an imagewhose resolution and depth of field have never been achieved in astereomicroscope before. With its fully apochromatic optics, largezoom range, and resolution performance, the Leica M205 FA canshow the viewer details that used to be invisible. The instrumentsalso feature the TripleBeam principle: A patented third beam pathreserved exclusively for fluorescence illumination delivers evenlyilluminated, reflex-free fields of view at all zoom settings.LeicaFor information +49-(0)-6441-29-2550www.leica-microsystems.com

3D Cell Proliferation AssayAlthough more scientists are growing cells in a three-dimensional(3D) culture that mimics in vivo conditions and allows the cells toorganize into complex structures, they have lacked a practical wayto evaluate cell proliferation in these cultures. Four new 3D cellproliferation kits include a core kit and three others that containbasement membrane extract, laminin I, and collagen I. Designedfor assessing the effects of compounds or genetic alteration on cellproliferation in 3D culture, the high throughput 96 stripwell formatsallow one-step quantitative analysis and colorimetric detection.Protocols are provided for tumorgenicity and cytotoxicity.TrevigenFor information 800-873-8443www.trevigen.com

Palm-Size ThermocyclerThe Palm PCR is a palm-size portable thermocycler with a widedynamic range capable of delivering polymerase chain reaction(PCR) amplified product in as little as 15 minutes. Palm PCR is abattery-powered, stand-alone device that delivers efficient andfast amplification for nearly all kinds of DNA samples, includingthe human genome. Even a single copy of DNA can be amplified in

as little as 25 minutes to an amount sufficient for typical agarose-gel detection. It can achieve uniformity of better than ±0.1˚Cand can run for more than four hours on a single charge of alithium-ion battery.Ahram BiosystemsFor information 650-224-8575www.ahrambio.com

Human Stem Cell KitsNew Human Stem Cell Nucleofactor Kits enable researchersto efficiently transfect various types of stem cells such asmesenchymal, hematopoietic, or neural stem cells withoutcompromising their potential for differentiation. With these kits,for single reactions and up to 96-well transfections, transfectionefficiencies of 60 percent to 80 percent with high viability can beachieved for stem cells. The kits’ optimized transfection conditionsallow the use of DNA, small interfering-RNA, or peptides.AmaxaFor information +49-221-99199-464www.amaxa.com

Immunodetection SystemThe Tropix Western-SuperStar Immunodetection System candetect proteins on protein immunoblots for up to 4.5 days afterthe substrate is added, compared with competitors’ kits in whichthe signal is gone after 4 hours. The kits make use of light-emission kinetics that rivals horseradish peroxidase-baseddetection chemistry.Applied BiosystemsFor information 800-327-3002www.appliedbiosystems.com/westernsuperstar

Pipetting FiltersBiohit Safe-Cone filters for the Biohit line of precision pipettorsoffer safe pipetting and prevent contamination of the pipettor andsample. The filters can prolong the life of the pipettor by protectingthe internal mechanisms. Pipettor parts are also protected fromvapors and liquids that could contaminate or damage internalcomponents. The filters are available in standard form for generalwork and as Safe-Cone Plus format for radioactive, cell culture,and molecular biology applications.BiohitFor information 800-922-0784www.biohit.com

Newly offered instrumentation, apparatus, and laboratory materials of interest to researchers in all disciplines in academic, industrial, and governmental organiza-tions are featured in this space. Emphasis is given to purpose, chief characteristics, and availabilty of products and materials. Endorsement by Science or AAAS ofany products or materials mentioned is not implied. Additional information may be obtained from the manufacturer or supplier.

Electronically submit your new product description or product literature information! Go to www.sciencemag.org/products/newproducts.dtl for more information.

New Products

Protein Immunoblot Imaging and AnalysisThe FluorChem Q Imaging System provides the sensitivity of chemiluminescence and the quantitativepower of fluorescence in one easy-to-use instrument. Designed with fast-lens technology, a peltier-cooled camera captures high-resolution images with a linear dynamic range that outperforms film, andwith speeds 10 times faster than a laser scanner. Equipped with three integrated excitation sources formulticolor blots, the FluorChem Q is compatible with Cy dyes, Alexa dyes, Q dots, and chemiluminescentprotein immunoblotting kits. This versatile system can also image fluorescent DNA and protein gels. Thesystem comes with intuitive software that automatically stores all experimental imaging protocols as wellas channel and filter settings.

Alpha InnotechFor information 510-483-962

www.alphainnotech.com

Our Innovation, Your Research — Shaping the Future of Life Science

Your Favorite Gene is a search tool that matches your gene of

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R&D SystemsYour Source for Stem Cell Reagents.Embryonic | Neural | Mesenchymal | Hematopoietic

For more information visit our website at www.RnDSystems.com/go/StemCells

Human ES cells were expanded in culture with orwithout MEF-Conditioned Medium (Catalog #AR005) and supplemented with FGF-basic. Cells inboth panels were stained with markers for undif-ferentiated cells including anti-human/mouseSSEA-4 (Catalog # MAB1435; red) and anti-humanOct-3/4 (Catalog # AF1759; green). Cells were coun-terstained with DAPI (blue). Experiments courtesy ofDr. Frank Soldner, NIH.

Expansion of ES Cells

NEW! The Mouse Mesenchymal Stem Cell FunctionalIdenti�cation Kit (Catalog # SC010) was used to pro-mote MSC di�erentiation into the adipogenic line-age and assess the phenotype using the includedanti-FABP4 antibody (red). The nuclei were counter-stained with DAPI (blue).

Identi�cation of Adipogenic Maturation

Di�erentiating Rat Cortical Stem Cells (Catalog #NSC001) were assessed with the Neural Stem CellFunctional Identi�cation Kit (Catalog # SC013) usingthe included anti-Nestin (green) and anti-Neuron-speci�c β-III Tubulin (red) antibodies. The nucleiwere counterstained with DAPI (blue).

Di�erentiation of NSCs

MEF-ConditionedMediumFGF-basic

UnconditionedMediumFGF-basic

EXPANSION Serum-free media & supplement kits specially designed for each stem cell type.

DIFFERENTIATIONGrowth factors & supplement kits formulated to direct stem cells along speci�c lineages.

IDENTIFICATION Antibody & primer panels to monitor stem cell di�erentiation.