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![Page 1: Takafumi Hamaoka, MD, PhD Faculty of Sport and Health Science, Ritsumeikan University, Kyoto, JAPAN MUSCLE RESEARCH WORK WITH BRITTON CHANCE FROM IN VIVO.](https://reader036.fdocuments.in/reader036/viewer/2022062321/56649eb55503460f94bbe32c/html5/thumbnails/1.jpg)
Takafumi Hamaoka, MD, PhDFaculty of Sport and Health Science,Ritsumeikan University,Kyoto, JAPAN
MUSCLE RESEARCH WORK WITH BRITTON CHANCE FROM IN VIVO MAGNETIC RESONANCE SPECTROSCOPY TO NEAR-INFRARED SPECTROSCOPY
Takafumi Hamaoka, MD, PhD
Kevin K. McCully, PhDUniversity of Georgia
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The first point of contact between BC
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The first point of contact between BC
• The first point of contact between BC and our research group was in late 1980’s in Hawaii Triathlon Race (Hawaii is a good place to visit as a vacation, but not as a field research, HOT!!).
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Prof. Iwane
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The first point of contact between BC
• I have heard that Prof. Iwane, my former supervisor in cardiology, had met one (presumably Dr. Pamela Douglas) of the cardiologists travelled from U of Penn to Hawaii, who had told BC a phenomenon of myoglobinemia (>103 fold post-race) after triathlon race lasting over 10 hours.
• Then, BC invited Iwane, who did not conducted basic science, but rather inclined to clinical science, to his lab and had him talk about long-distance race and myoglobinemia. Inviting Iwane was puzzling to us (and also himself) at that time.
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• But, I have understood why BC had contacted Iwane, later at a table over a dinner with BC. We have already chased and tested exhaustive triathletes immediately after the race for changes in near-infrared myoglobin and hemoglobin signals using off-line mini-RunMan with a battery for a field study which Chris Albani had built. (I have even heard that BC wanted to ship a magnet from Japan to Philadelphia and temporarily install the magnet in Hawaii on the way for testing exhaustive muscle of triathletes!!)
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• BC was trying to calculate on a napkin how much intramuscular myoglobin was released to the blood stream and urine over the race based on Iwane’s research data. The amount of Mb, which has been released to extramuscular space, would correspond to the decrease in muscle NIR signal post-race. In a melting human muscle model, BC wanted to differentiate Mb signal from overall NIR signals which, I believe, still remains to be solved.
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Conventional Muscle Study
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Muscle biopsy
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Happy with muscle biopsy?
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METHODOLOGIES
Tc
MRS
NIRS
MRI
US
φ
L
Mus
cle V
O2 (f
old of
resti
ng)
・
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Muscle Cell
Oxidative Metabolism
ADP, Pi PCr
NAD/NADHMotor Nerve
Ach.
Muscle Pump
Symp. Nerve
Metabolites
O2
Cardiac Output
N.A.
NOActive Muscle
Contraction
Viscera
Brain ?
?
NOHbO2
Non-active Limbs
Temperature
Noninvasive Measures of Muscle Metabolism and Circulation
ATP
N.A.
VO2.Mit.
Blood FlowVO2.
Blood Flow
VO2.
+
-
+ ++
+
+
-
-
-
Less Active Organs
NIRS
Doppler
MRS
Respr. Gas Analysis
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Control of Oxidative Metabolism 1. Kinetic Control by ADP (Chance : Proc.Natl.Acad.Sci., 1985)
[ADP]=([ATP]/[PCr])[Creatine] / (Kck
[H+])
Steady-State condition proposed by B. Chance
ATP, [H +]=Constant, Creatine=Pi
then, [Pi] / [PCr] = [ADP] Kck
[H+] / [ATP]
[Pi] / [PCr]= K'[ADP]
2. Thermodynamic Control (Meyer : Am. J. Physiol., 1988)
Δ GATP
=Δ G0ATP
- RTlog([ATP]=([ADP]/[Pi])
Δ GATP
=K- k[PCr] 20%of Tc <PCr < 75% of Tc
What stimulates mitochondrial oxygen consumption?
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Time (sec)
84072060048036024012000
26
27
28
29
30
31
32
0
20
40
60
80
100
PC
r (m
M)
m-O
2 (
% o
f re
sti
ng
)
Arterial Occlusion
Changes in Muscle PCr and HbO2 during Resting Arterial Occlusion
BMR NMR
VO2 REST NIR
Hamaoka et al., JAP, 1996
0
20
40
60
80
100PvO2
PintO2
Time (min)
PO
2 (T
orr)
242220181614121086420
Arterial Occlusion
Fig. 3. Changes in muscle interstitial PO2 (PintO2) and venous PO2 (PvO2)
during resting arterial occlusion.
Hamaoka et al., J. Biomed. Opt., 2000
There is no oxygen gradient betweenvenous and interstitial compartments
Functional anoxic condition
Basal metabolic rate measurement
= 8.2 M ATP/sec
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m-O
2 (%
of
res
tin
g)
141210864200
20406080
100
GripArterial Occlusion
Arterial Occlusion
S1 S2
Time (min)
VO2 REST NIR VO2 EX
NIR
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1211109876543210
95
100
Time (sec)
m-O
2 (%
of
rest
ing) R^2 = 0.971
75
40
45
50
55
60
65
70
R^2 = 0.980
Post-Exercise
Resting
Arterial Occlusion
S1
S2 End of Exercise
= 8.2 M ATP/sec
= 82 M ATP/sec
x 10
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Four different intensities of exercisefor changes in both muscle oxygen consumptionand phosphorus metabolites
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50454035302520150
2
4
6
8
10
12
ADP ( µM)
O2 C
onsu
mpt
ion
(µM
O2/s
ec)
y = - 6.6752 + 0.43421x R^2 = 0.989
Relation between VO2 measured by NIRS and ADP measured by MRS
J. Appl. Physiol. Hamaoka et al.1996
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3230282624222020
0
2
4
6
8
10
12
PCr (mM)
y = 30.016 - 0.90554x R^2 = 0.993
O2
Con
sum
ptio
n (µ
MO
2/se
c)
Relation between VO2 measured by NIRS and PCr measured by MRS
J. Appl. Physiol. Hamaoka et al.
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Display and self-powered sensor
NIRS imager with display
Energy harvesting
sensor
flexible display
energy harvesting module,data logging module
sunlight or LED
Flexible display
3) What is the major challenge to achieving this?
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Summary
BC has conducted MRS and NIRS research on elite athletes and a number of chronic health conditions, including patients with chronic heart failure, peripheral vascular disease, and neuromuscular myopathies.
As MRS and NIRS technologies are practical and useful for measuring human muscle metabolism, we will strive to continue Chance’s legacy by advancing muscle MRS and NIRS studies.
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BC,
thank you for your time for us!!
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Triathlete100km Runner
TennisPlayer
Middle Aged
Faculty of Sport and Health Science,Ritsumeikan University,Kyoto, JAPAN