TABLE S1. Primers used in mutagenesis of Q motif of FANCJ and BLM

2
Primers Sequence (5’-3’) FANCJ-Q25AF GCTTACCCGTCAGCGCTTGCTATGATG FANCJ-Q25AR CATCATAGCAAGCGCTGACGGGTAAGC FANCJ-QF GTGGGGTGAAGATTTACCTTGCTATGATGAATTC FANCJ-QR GAATTCATCATAGCAAGGTAAATCTTCACCCCAC BLM-Q672RF CATAATTTTAGAACTAATCGGCTAGAGGCGATCAATGC BLM-Q672RR GCATTGATCGCCTCTAGCCGATTAGTTCTAAAATTATG BLM-Q672AF CATAATTTTAGAACTAATGCGCTAGAGGCGATCAATGC BLM-Q672AR GCATTGATCGCCTCTAGCGCATTAGTTCTAAAATTATG TABLE S1. Primers used in mutagenesis of Q motif of FANCJ and BLM

description

TABLE S1. Primers used in mutagenesis of Q motif of FANCJ and BLM. TABLE S2. DNA substrates or oligonucleotides used in this study. 5’. 26. DC26:TTTTTTTTTTTTTTTTTTTTTTCCCAGTAAAACGACGGCCAGTGC T STEM 25:GCGGTCCCAAAAGGGTCAGTGCTGGCATTTTGCTGCCGGTCACG. I I I I I I I I I I I I I I I I I I I. - PowerPoint PPT Presentation

Transcript of TABLE S1. Primers used in mutagenesis of Q motif of FANCJ and BLM

Page 1: TABLE S1. Primers used in mutagenesis of Q motif of FANCJ and BLM

Primers Sequence (5’-3’)

FANCJ-Q25AF GCTTACCCGTCAGCGCTTGCTATGATG

FANCJ-Q25AR CATCATAGCAAGCGCTGACGGGTAAGC

FANCJ-QF GTGGGGTGAAGATTTACCTTGCTATGATGAATTC

FANCJ-QR GAATTCATCATAGCAAGGTAAATCTTCACCCCAC

BLM-Q672RF CATAATTTTAGAACTAATCGGCTAGAGGCGATCAATGC

BLM-Q672RR GCATTGATCGCCTCTAGCCGATTAGTTCTAAAATTATG

BLM-Q672AF CATAATTTTAGAACTAATGCGCTAGAGGCGATCAATGC

BLM-Q672AR GCATTGATCGCCTCTAGCGCATTAGTTCTAAAATTATG

TABLE S1. Primers used in mutagenesis of Q motif of FANCJ and BLM

Page 2: TABLE S1. Primers used in mutagenesis of Q motif of FANCJ and BLM

DNA substrate/Oligonucleotides

Sequence (5’-3’)

Forked duplex

TP-G4 TP: TGGACCAGACCTAGCAGCTATGGGGGAGCTGGGGAAGGTGGGAATGTGA

X12-1-52-BIOT13 TTTTTTTTTTTTTTTTGACGCTGCCGAATTCTGGCTTGCTAGGACATCTTTG-BiodT-CCACGTTGACCCG

45-mer (DC26) TTTTTTTTTTTTTTTTTTTTTTCCCAGTAAAACGACGGCCAGTGC

DC26: TTTTTTTTTTTTTTTTTTTTTTCCCAGTAAAACGACGGCCAGTGC

TSTEM25: GCGGTCCCAAAAGGGTCAGTGCTGGCATTTTGCTGCCGGTCACGI I I I I I I I I I I I I I I I I I I

5’

3’

TABLE S2. DNA substrates or oligonucleotides used in this study

BiodT represents an internal biotin conjugated dT.

26

19 bp

5’

3’ 25


PickMutant® Site -directed Mutagenesis Kit€¦ · PickMutant Site -directed Mutagenesis kit is a based -PCR mutagenesis method so the design of primers is essential to obtain the

PickMutant® Site -directed Mutagenesis Kit€¦ · PickMutant Site -directed Mutagenesis kit is a based -PCR mutagenesis method so the design of primers is essential to obtain the

Tnt1 Retrotransposon Mutagenesis: A Tool for Soybeanparrottlab.uga.edu/parrottlab/Mutagenesis/tnt/Cuietal2011.pdf · Tnt1 Retrotransposon Mutagenesis: A Tool for Soybean Functional

Tnt1 Retrotransposon Mutagenesis: A Tool for Soybeanparrottlab.uga.edu/parrottlab/Mutagenesis/tnt/Cuietal2011.pdf · Tnt1 Retrotransposon Mutagenesis: A Tool for Soybean Functional

QTL analysis / Mutagenesis

QTL analysis / Mutagenesis

Recombinant engineering of gene function: mutagenesis I. Why mutagenize? II. Random mutagenesis, mutant selection schemes III. Site-directed mutagenesis,

Recombinant engineering of gene function: mutagenesis I. Why mutagenize? II. Random mutagenesis, mutant selection schemes III. Site-directed mutagenesis,

FANCJ suppresses microsatellite instability and ...genesdev.cshlp.org/content/29/24/2532.full.pdf · FANCJ suppresses microsatellite instability and lymphomagenesis independent of

FANCJ suppresses microsatellite instability and ...genesdev.cshlp.org/content/29/24/2532.full.pdf · FANCJ suppresses microsatellite instability and lymphomagenesis independent of

FANCJ regulates the stability of FANCD2/FANCI proteins and ...Map of FANCJ functional domains, protein interaction sites and mutations. This figure illustrates the various domains

FANCJ regulates the stability of FANCD2/FANCI proteins and ...Map of FANCJ functional domains, protein interaction sites and mutations. This figure illustrates the various domains

ARDEX Primers ARDEX PRIMERS...ARDEX Primers AF 133 11/14 Key Features ARDEX PRIMERS • Solvent-free • Concentrated primer - dilutes with water • Seals the substrate • Interior

ARDEX Primers ARDEX PRIMERS...ARDEX Primers AF 133 11/14 Key Features ARDEX PRIMERS • Solvent-free • Concentrated primer - dilutes with water • Seals the substrate • Interior

Primers dies

Primers dies

Transposons Mediated Mutagenesis

Transposons Mediated Mutagenesis

Primers dies

Primers dies

GeneArt Site-Directed Mutagenesis PLUS Kit...Primer and Construct Design Tool is an intuitive, web-based tool to guide you when you are designing your oligonucleotide primers for multisite

GeneArt Site-Directed Mutagenesis PLUS Kit...Primer and Construct Design Tool is an intuitive, web-based tool to guide you when you are designing your oligonucleotide primers for multisite

Mutagenesis Techniques (PDF)

Mutagenesis Techniques (PDF)

Nondegenerate Saturation Mutagenesis · 2020. 3. 9. · SATURATION MUTAGENESIS METHODS 3.1 Core techniques in saturation mutagenesis Whether saturation mutagenesis is degenerate,

Nondegenerate Saturation Mutagenesis · 2020. 3. 9. · SATURATION MUTAGENESIS METHODS 3.1 Core techniques in saturation mutagenesis Whether saturation mutagenesis is degenerate,

Genome-Scale Mutagenesis

Genome-Scale Mutagenesis

Supporting Online Material for · The initiation codon ATG in the comK-96aa-cfp construct was mutated to GTG by PCR mutagenesis using Primers 10 and 11, and the plasmid pED1022 as

Supporting Online Material for · The initiation codon ATG in the comK-96aa-cfp construct was mutated to GTG by PCR mutagenesis using Primers 10 and 11, and the plasmid pED1022 as

Mouse mutagenesis

Mouse mutagenesis

Vmm Primers

Vmm Primers

transposon mediated mutagenesis

transposon mediated mutagenesis

Site-directed Mutagenesis

Site-directed Mutagenesis

1 Directed Mutagenesis and Protein Engineering. 2 Mutagenesis Mutagenesis -> change in DNA sequence -> Point mutations or large modifications Point mutations.

1 Directed Mutagenesis and Protein Engineering. 2 Mutagenesis Mutagenesis -> change in DNA sequence -> Point mutations or large modifications Point mutations.