T. Trimpe 2005 http://sciencespot.net/

Microscope HistoryAntony Van Leewenhoek is known as the ___________________. His first microscopes were in use in the 17th century.

Robert Hooke is noted as the first person to realize that all living things contained ___________.

Zachariah Janssen created the first___________________, which is what we use in class.

Father of Microscopy

cells

compound microscope

Microscope Safety

Always carry the microscope by holding the following parts: 1. 2.

Never touch the ___________. This will smudge them allowing you to not see clearly.

After the microscope has been on a while, the ___________ will be hot. Be careful as you are putting the microscope away.

ArmBase

lenses

light/lamp

4X to 40X

Microscope LensesEyepieceThe eyepiece is called the _________________ lens.

Magnification of the eyepiece = _____________

Objective Lenses

ocular

10x

Lens Magnification Coordinates with the color

Total Magnification when looking through the eyepiece

Low Power

Medium Power

High Power

10x

4x

40x

Red

Yellow

Blue

10 X 4 = 40XEyepiece Low Power

10 X 10 = 100XEyepiece Med. Power

10 X 40 = 400XEyepiece High Power

Visual Field/ Field of ViewWhat is it?It is the circle of light you see when you look into the microscope.

We can see better details with higher the powers of magnification, but we cannot see as much of the image.

Which of these images would be

viewed at a higher power of

magnification?

Visual Field Comparison

Low PowerMedium Power High Power

Use the words below to fill in the blanks

• Objecs that you will study, such as cells, are much smaller than one millimeter. A millimeter can be divided into 1,000 parts. Each part is called a __________.

• 1 mm = __________• If the visual field measures 3.5 mm, how many

microns is that? ____________________• As you increase your magnification, the amount of

what you see in the visual field gets __________. The detail of what you see in the visual field ____________.

micron

1,000 microns

3.5x 1,000= 3,500 microns

smaller

increases

Smaller 1,000 microns increasesmicron 3,500 microns

Let’s give it a try ...1 – Turn on the microscope and then rotate the nosepiece to click the red-banded objective into place.

2 – Place a slide on the stage and secure it using the stage clips. Use the coarse adjustment knob (large knob) to get it the image into view and then use the fine adjustment knob (small knob) to make it clearer.

4 – When you are done, turn off the microscope and put up the slides you used.

3 – Once you have the image in view, rotate the nosepiece to view it under different powers. Draw what you see on your worksheet!

Be careful with the largest objective! Sometimes there is not enough room and you will not be able to use it!