t 004115
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sigma-aldrich.com/supelco Application Report 115 Analysis of Tranquilizer Fentanyl Citrate Using Ascentis TM C18 This application demonstrates the suitability of Ascentis C18 for the efficient elution of the tranquilizer fentanyl by HPLC. Key Words Ascentis C18, fentanyl, 990-73-8, F5886, 581324-U, tranquilizer Conditions column: Ascentis C18, 15 cm x 4.6 mm I.D., 5 μm particles (581324-U) mobile phase: 67:33, 10 mM monobasic potassium phosphate (pH 3.0 with phosphoric acid):acetonitrile flow rate: 1 mL/min. temp.: 35 °C det.: UV at 215 nm injection: 10 μL sample: 20 μg/mL in mobile phase Structures Fentanyl - G002436 Author: Hugh Cramer Acquisition System: Waters Alliance 2690 ID 9371 Notebook Reference: 1495-09 Peak IDs 1. Fentanyl G002435 ©2004 Sigma-Aldrich Co. T004115 Ascentis is a trademark of Sigma-Aldrich Co. 1 N N O 0 2 4 6 8 10 Min
description
t 004115
Transcript of t 004115
sigma-aldrich.com/supelco
Application Report 115
Analysis of Tranquilizer Fentanyl Citrate Using AscentisTM C18This application demonstrates the suitability of Ascentis C18 for the efficient elution of the tranquilizer fentanyl by
HPLC.
Key WordsAscentis C18, fentanyl, 990-73-8, F5886, 581324-U, tranquilizer
Conditionscolumn: Ascentis C18, 15 cm x 4.6 mm I.D., 5 µm particles (581324-U)
mobile phase: 67:33, 10 mM monobasic potassium phosphate (pH 3.0 withphosphoric acid):acetonitrile
flow rate: 1 mL/min.temp.: 35 °C
det.: UV at 215 nminjection: 10 µL
sample: 20 µg/mL in mobile phase
StructuresFentanyl - G002436
Author: Hugh Cramer
Acquisition System: Waters Alliance2690 ID 9371
Notebook Reference: 1495-09
Peak IDs
1. Fentanyl
G002435
©2004 Sigma-Aldrich Co. T004115
Ascentis is a trademark of Sigma-Aldrich Co.
1
N
N
O
0 2 4 6 8 10Min
