Szeged - Timisoara axis for the safe food and feed Acronym: SZETISA 1
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Szeged - Timisoara axis for the safe food and feed
Acronym: SZETISA 1
HURO / 0901/147/2.2.2
Szeged University
Banat University of Agricultural Sciences and Veterinary Medicine, Timisoara
January, 26th - 27th, 2012
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Determination of microelements as affected by microbial toxins in wheat
2 wheat cultivars artificially infected with 2 Fusarium strains of low and high activities, respectively, in Szeged (Cereal Research Institute).
Wheat specimen spontaneously and naturally infected - collected in Romania
The analyzed material
Artificially infected wheat cultivars – moment 0 – Fusarium strain identification
the samples were negatives
The Fusarium attack was evaluated
in different organs:
parts1 grains2 chaf3 culm4 flag leaf
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GMO presence
Fusarium detection
Toxin genes identification
Corn + Soy bean
Fusarium graminearumFusarium culmorumFusarium proliferatumFusarium verticiloides
TRI 7 DONTRI 13 DONTRI 7 NIVPQF5 - fumonisin
8 samples of commercial feed samples
Wheat specimen spontaneously and naturally infected - collected in Romania
Fusarium detectionToxin genes identification
Collected from 2010 harvest
7 wheat samples, collected from three Romanian counties
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WHEAT SAMPLES
18 wheat samples, collected from two Romanian counties
Fusarium detectionToxin genes identificationToxin quantification
Flag leaf
Culm
Ear Chaf
Grain Fusarium quantification
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The analyzed material
DETERMINATION OF MICROELEMENTS AS AFFECTED BY MICROBIAL TOXINS IN MAIZE.
2 corn cultivars - genetically modified (Bt) and conventional from different locations
5 maize samples from last year’s harvest 4 conventional varieties YG – genetically modified maize
GMO presence
Fusarium detection
Toxin genes identification
Maize samples from this year harvestgenetically modified maize – 14 from different farms
GMO presence
Fusarium detection
Toxin genes identification
Toxin quantification
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SIGMA
SATELIT
Two month treatment
The plants were grown in the green house
ALFALFA metal treatment
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Untreated - control
Pb 50 Cd 1 Cu 100 Fe 100
100 3 250 250
250 10 500 500
500 20
Untreated - control
Pb 50 Cd 1 Cu 5 Fe 5100 3 10 10250 10 50 50
Dried materials
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INCREASE OF INFRASTRUCTURE
COOLING CENTRIFUGE - SIGMA
Multi-Sample Micro-Volume UV-Vis Spectrophotometer
The tender was organizedThe contract was signedThe equipment was installed
The tender was organizedThe contract was signedThe equipment was installed
BIOANALYZER
The tender was organized
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Ioja-Boldura Oana-Maria, Popescu Sorina
A molecular approach for screening of toxigenic Fusarium graminearum in feedstuff
Bulletin of University of Agricultural Sciences And Veterinary Medicine, Volume 68 (1-2), pag. 422, Cluj-Napoca 2011, ISSN 1843-5262
COMUNICATION ACTIVITIES
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THANK YOU FOR YOUR ATTENTIONS!