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Synopsis Presentation

Presented By

Dr Vini Sharma

First Year Postgraduate studentGuide : Head of Department :

Dr. Suparna Ganguly Saha Dr. Suparna Ganguly Saha

Professor & HOD Professor & HOD

DEPARTMENT OF CONSERVATIVE DENTISTRY & ENDODONTICSCOLLEGE OF DENTAL SCIENCES AND HOSPITAL

RAU (INDORE)

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Title of Research

Evaluation of tubular penetration depth of a sealer after

irrigation with four root canal irrigants of different origin-

6% Morinda Citrifolia juice, 0.2% Chitosan, 17% EDTA,

3% NaOCl-an in vitro study

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Aim of study

• The aim of this study is to examine in-vitro

penetration of a sealer into dentinal tubules after using

four different irrigating solutions (6% Morinda citrifolia

juice , 0.2% chitosan , 17% EDTA and 3% NaOCl)

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Objectives of studyA ) To evaluate the penetration depth of a sealer into the dentinal

tubules of the samples after irrigation with 6% Morinda citrifolia juice.

B ) To evaluate the penetration depth of a sealer into the dentinal tubules of the samples after irrigation with 0.2% Chitosan.

C ) To evaluate the penetration depth of a sealer into the dentinal tubules of the samples after irrigation with 17% EDTA.

D ) To evaluate the penetration depth of a sealer into the dentinal tubules of the samples after irrigation with 3% NaOCl.

E ) To Compare the penetration depth of a Root canal sealer into the dentinal tubules after irrigation with four root canal irrigants of different origin.

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Materials

1. CUTTING DIAMOND IMPREGNATED DISC( for decoronation at the level of CEJ)

2. HARD TISSUE MICROTOME3.  NUMBER 10 /15 K FILE ( for working length

establishment)4.  STRAIGHT HANDPIECE5.  MICROMOTOR6.  ROTARY FILE SYSTEM7.  ENDOMOTOR8.  SYRINGE with side vented needle

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10.  IRRIGATING SOLUTIONa) 6 % Morinda citrifolia juiceb)  0.2% Chitosanc)  17 % EDTAd) 3 % NaOCl

11.  ENDOACTIVATOR12. PAPER POINTS13.  ROOT CANAL SEALER14. NORMAL SALINE15. GUTTA-PERCHA16. SCANNING ELECTRON MICROSCOPE17. INTERMEDIATE RESTORATIVE MATERIAL

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Sample size

Sixty freshly extracted premolars with mature apices will be

collected from the Department of Oral and Maxillofacial

Surgery, College of Dental Sciences and Hospital Rau,

Indore.

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Inclusion Criteria

• Single rooted teeth with single canal.

• Teeth with intact crown.

• Teeth with fully mature apices.

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Exclusion Criteria

• Roots with aberrant morphology –

Teeth with curved

roots.

Teeth with open apices.

• Teeth with caries.

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Method of study: Sixty freshly extracted human premolars will be collected (permanent, non-carious, single rooted

with fully developed apices) after taking preoperative radiograph

 Stored in 10%ethyl alcohol solution and four days

prior to experiment samples will be stored in 1% NaOCl

 Decoronation will be done at the level of CEJ

(diamond disc) 

 

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Root canal will then be accessed 

Working length will be determined (using 10/15 K file)

Cleaning and shaping will be done with the help of rotary file system following

manufacturer’s instructions

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Each canal will be irrigated with 2ml of 3% NaOCl using a syringe with a closed end side vented needle with insertion depth 1mm short of working length

Samples will then be randomly divided into four groups  

Group A Group B Group C Group D (n=15) (n=15) (n=15) (n=15) Irrigated with Irrigated with Irrigated with Irrigated with 5ml of 6% 5ml of 0.2% 5ml of 17% 5ml of 3% morinda

Chitosan for EDTA for NaOCl forcitrifolia for 2min/canal 2min/canal 2min/canal

2min/canal

  

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Each group will be irrigated finally by 2ml of Normal Saline for 2min

 

Root Canals will then be dried using paper points.

Obturation will be done using gutta-percha cones with the sealer placed according to

manufacturer’s instructions

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Orifices will be sealed with intermediate restorative material

 Radiographs will be taken to check for voids in

obturation 

All samples will be stored at 37°C and 100% humidity to allow sealers to set for a specified period of time.

 Then all samples will be mounted on acrylic blocks in

order to stabilize the samples .

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Transverse Sectioning will be done at a distance of 2mm, 5mm and 8 mm from the root apex using a hard tissue

microtome. 

Gold sputtering will be done (for making the samples conducting)

Scanning electron microscopic evaluation will be done to see the maximum sealer penetration

 Statistical analysis will be done

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For preparation of 0.2% Chitosan solution, 0.2g of Chitosan +100ml of acetic acid

stirred for 2 hrs using magnetic stirrer

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Statistical analysis

• Statistical analysis will be carried out using

comparison of mean parametric values using One-

Way ANOVA along with Post-hoc Tukey test.

• Non-parametric values will be compared using

Pearson's Chi-square test or Z test.

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References• Koppolu M, Eragamreddy S, Anumula L et al. Comparison of Chitosan and Morinda

citrifolia on smear layer removal: An in-vitro study. Sch J Dent Sci. 2015;2(2A):132-6

• Shenoy A, Ahmaduddin, Nagesh B et al. Effect of final irrigating solution on smear

layer removal and penetrability of the root canal sealer. J Conserv Dent 2014;17(1):40-

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• Saghiri MA, Garcia-Godoy F, Asgar K, Lofti M. The effect of Morinda citrifolia juice as

an endodontic irrigant on smear layer and microhardness of root canal dentin. Oral Sci

Int 2013;10:53-7.  

• Koch K, Brave D, Nasseh AA. A review of bioceramic technology in endodontics. CE

Article. 2012;4:6-12.

• Chadha R, Taneja S, Kumar M et al. An in vitro comparative evaluation of depth of

tubular penetration of three resin-based root canal sealer. J Conserv Dent 2012;15(1):18-

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• Kossev D, Stefanov V. Ceramics-based sealers as new alternative to currently used

endodontic sealers. Roots 2009;1:42-8

• Murray PE, Farber RM, Namerow KM, et al. Evaluation of Morinda citrifolia as an

endodontic irrigant. J Endod. 2008;34:66–70

• Teixeira CS, Felippe MC, Felippe WT. The effect of application time of EDTA and

NaOCl on intracanal smear layer removal: an SEM analysis. Int Endod J.

2005;38(5):285-90. 

• Aktener BO, Bilkay U. Smear layer removal with different concentrations of EDTA-

ethylenediamine mixtures. J Endod. 1993 May 31;19(5):228-31.

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THANK YOU