Synergetic mechanism of the effects of anthrax and dental microbial plaque

fragments at the design (fashion) of bio-weapons and/or cancer treatment

By Antonio Schütz

“Is not necessary to contaminate all an island to proof this mechanism!”

(TN2/2482533)

“The game is equalized”

Synergetic effect of Anthrax toxin and dental microbial plaque fragments (general mechanism)

• Increases of the synthesis and

secretion of cytokines production by

macrophages

• Increases the effects of LF

• Increases the effects of EF

• Increases spores germination to

become vegetative cells within

macrophages

• Impairs phagocytosis in macrophages

• Increase of macrophages phagocytose

and lyse endospores

• Cancer Treatments

– Oncogenic proteins (Ras) activate MAPKs

– Expression of matrix metalloproteases

– Apoptosis induction

• Improves the formulation of anthrax (aerosol and particularly paste powder – dry paste)

• Increases the secretion and synthesisof free radicals

Hypothension

Septicemia“Trojan horse”

Monocytes

• Chemokynes (MIP, IP-10, MCP-1)

[IL-8]IL-6

•Decreased lymphocytes function [RANTES]

[cytokines and growth factors vase-actives]

Enzime NADPH oxidase iNOS, MPO

Free Radicals: NO∙, O2

-

HOCL∙ RHNCL∙

Necrosis Apoptosis

Mustard gas Dental plaque chemoatracts macrophages

LPS Mustard gas

LPS Mustard gas

The anthrax bacteria produce the anthrax toxin components—protective antigen, edema factor, and lethal factor. Protective antigen (PA) binds to the anthrax toxin receptor (ATR) on cell surfaces and forms a protein-receptor complex that binds lethal factor (LF) and edema factor (EF). The toxins enter the cell and kill it. Most anthrax fatalities are caused by the irreversible effects of the anthrax toxins on cells and tissues.

Synergistic effect of Anthrax toxin and dental microbial plaque fragments

• Acidogenic dental

bacteria Increase the

effects of PA

Septic

Apoptosis: LF (anthrax) and LPS (dental plaque) induce apoptosis via activation TLRShock

Endotoxin of Gram-negatives of dental plaque

cytopl. mem.

peptidoglycan

outer mem.

Gram-negative

cell

Lipid A Core polysaccharide

O sidechain

The toxic partHelps solubilise Lipid A Somatic antigen

Lipopolysaccharide(LPS)

Endotoxins of Gran-negatives from dental microbial plaque increase the synthesis and secretion of tumour necrosis factor IL-1, NO, [PGs] as well as their effects (death, apoptosis, edema) induced by anthrax toxin.

TNF-α, IL-β [PGs], ON increasing death,

apoptosis and edema]

Synergetic activation of NOS

Actives EF

cellsFree radicals

Immunesuppression

Oxidation of thiois

Lipid peroxidation

Indomethacin [PGs] Interation with β-glucan

[TNF-α, IL-1]

Anthrax Dental plaque

SepticemiaDeath

LPS

Methotrexate [folate] Mustard gas

Apoptosis

Ulceration of gastroinstestinal mucosa Nephrotoxicity

Increase the effect of EF

[ET-1]

Edema

Synergistic activation of NOS increase the production of GMPc

Death

Indomethacin [PGs]LPS [PGs]

ABthrax specifically recognizes and neutralizes protective antigen (PA). By neutralizing PA, ABthrax effectively blocks the binding of PA to cell surfaces and prevents the anthrax toxins from entering and killing the

cell.

• Evasion of complement and immune response by structural components ( polysaccharides, sialic acid, hyaluronic, LPS) and products of synthesis and secretion of microorganisms of the dental plaque (factor H, proteases, coagulases, A and M proteins, soluble antingen), such as stafilococcus, streptococcus able to block the production of ABthrax antibodies (vaccination)

Chemical components that increase immune-suppressor and/or citotoxic effects

• Chemical substances with structure similar to Indomethacin and Methotrexate.

• Triazole pesticides - Inhibition of biosynthesis of steroids - Low toxicity - Ineffective against spores - Peak in 14 days (at plants) - Variable product formulation: dry and liquid flowables,

wettable powders and emulsifiable concentrates

• Mustard Gas

“The smart bomb”• Nanocells:

- A new nanoparticle drug-delivery system that makes it possible to combine drugs (anthrax, dental plaque and heterocyclic chemical) with different mechanisms of action in the same particle, and to design their temporal release with objective of obtaining the synergetic effect. - Is possible to rise the effects of anthrax toxins with simple addiction of cytokines (IL-1, TNF…), chemocynes (MIP, IP-10,

MCP-1...) and growth factors ( ERGF…), LPS at concentration greater than 100 ng/mL, ON, PGs etc… - In my experience with dental plaque (100 mG/mL) has its toxicity increased at the short time by indomethacin and methotrexate, possibly the same occur with chemical with similar structure.

Conclusion• As at the construction of a dress, the first phase is the

design. The second is the selection of the color and texture of the tissue (laboratorial and clinical tests) that will be used in its construction; the third, the construction by competent dressmakers. So, the idea of the design is presented. Now, desire that the colleagues test it and elaborate the final product. If a dress originally draw for a fest is modified and used at a married, is secondary. For the designer what really import is the design of the idea. Desire this fashion may be useful for you and particularly to that (TN2/2482533)

incited me the idea of this design. Thank you very much.